Catalysts based on Ni-Me (Me = Co, Nb) supported on γ-Al2O3 were prepared by the coprecipitation-chemical deposition method via reflux, and hydrothermal method for oxidative dehydrogenation of ethane. For coprecipitation method, ammonia and urea were used as precipitating agents. In both methods, the Ni content was 30 wt.%, whereas that of Me (Co, Ni) was 5 wt.%. The activity and selectivity towards ethylene appeared to depend strongly on the composition of the catalysts and, interestingly, on the preparation conditions. In the present article, it is shown that mixed hydrothermal samples using urea showed the best performance. Specifically, the hydrothermally prepared NiNb/γ-Al2O3 sample was the one that showed the highest values. The introduction of Nb to Ni framework allowed to enrich with nucleophilic oxygen sites displayed via X-ray photoelectronic spectra (XPS) which lead to the formation of low reducible and selective sites. NiMe Co γAl2O3 γAlO γ Al2O3 Al O γ-Al2O coprecipitationchemical chemical reflux ethane agents methods 3 wt wt.% (Co interestingly conditions article performance Specifically NiNb/γAl2O3 NiNbγAl2O3 NiNbγAlO NiNb/γ NiNb NiNb/γ-Al2O values Xray X ray XPS (XPS γAl γAl2O AlO Al2O wt. NiNb/γAl2O NiNbγAl NiNbγAl2O NiNbγ

Abstract Objective To evaluate the risk factors for postpartum hemorrhage (PPH) according to the Robson Classification in a low-risk maternity hospital. Methods We conducted retrospective cohort study by analyzing the medical records of pregnant women attended in a low-risk maternity hospital, during from November 2019 to November 2021. Variables analyzed were: maternal age, type of delivery, birth weight, parity, Robson Classification, and causes of PPH. We compared the occurrence of PPH between pregnant women with spontaneous (Groups 1 and 3) and with induction of labor (2a and 4a). Chi-square and Student t-tests were performed. Variables were compared using binary logistic regression. Results There were 11,935 deliveries during the study period. According to Robson’s Classification, 48.2% were classified as 1 and 3 (Group I: 5,750/11,935) and 26.1% as 2a and 4a (Group II: 3,124/11,935). Group II had higher prevalence of PPH than Group I (3.5 vs. 2.7%, p=0.028). Labor induction increased the occurrence of PPH by 18.8% (RR: 1.188, 95% CI: 1.02-1.36, p=0.030). Model including forceps delivery [x2(3)=10.6, OR: 7.26, 95%CI: 3.32-15.84, R2 Nagelkerke: 0.011, p<0.001] and birth weight [x2(4)=59.0, OR: 1.001, 95%CI:1.001-1.001, R2 Nagelkerke: 0.033, p<0.001] was the best for predicting PPH in patients classified as Robson 1, 3, 2a, and 4a. Birth weight was poor predictor of PPH (area under ROC curve: 0.612, p<0.001, 95%CI: 0.572-0.653). Conclusion Robson Classification 2a and 4a showed the highest rates of postpartum hemorrhage. The model including forceps delivery and birth weight was the best predictor for postpartum hemorrhage in Robson Classification 1, 3, 2a, and 4a. (PPH lowrisk low hospital 201 2021 age parity Groups . 4a) Chisquare Chi square ttests t tests performed regression 11935 11 935 11,93 period Robsons s 482 48 2 48.2 5,750/11,935 575011935 5 750 261 26 26.1 3,124/11,935. 312411935 3,124/11,935 124 3,124/11,935) 3.5 35 (3. vs 27 7 2.7% p=0.028. p0028 p p=0.028 0 028 p=0.028) 188 18 8 18.8 RR (RR 1188 1.188 95 CI 1.021.36, 102136 1.02 1.36, 02 36 1.02-1.36 p=0.030. p0030 p=0.030 030 p=0.030) x23=10.6, x23106 x x2 =10.6, 10 6 [x2(3)=10.6 OR 726 7.26 95%CI 95CI 3.3215.84, 3321584 3.32 15.84, 32 15 84 3.32-15.84 R Nagelkerke 0011 011 0.011 p<0.001 p0001 001 x24=59.0, x24590 4 =59.0, 59 [x2(4)=59.0 1001 1.001 95%CI1.0011.001, 95CI10011001 95%CI:1.001-1.001 0033 033 0.033 area curve 0612 612 0.612 0.5720.653. 05720653 0.572 0.653 572 653 0.572-0.653) 20 202 1193 93 11,9 48. 5,750/11,93 57501193 75 26. 31241193 3,124/11,93 12 3. (3 2.7 p002 p=0.02 18. 118 1.18 9 021 1.021.36 10213 102 1.0 136 1.36 1.02-1.3 p003 p=0.03 03 x23 x23=10.6 x2310 106 =10.6 [x2(3)=10. 72 7.2 3215 3.3215.84 332158 332 3.3 1584 15.84 3.32-15.8 01 0.01 p<0.00 p000 00 x24 x24=59.0 x2459 590 =59.0 [x2(4)=59. 100 1.00 CI1 95%CI1.0011.001 95CI1001100 95%CI:1.001-1.00 003 0.03 061 61 0.61 5720 0.5720.653 0572065 0572 0.57 0653 0.65 57 65 0.572-0.653 119 11, 5,750/11,9 5750119 3124119 3,124/11,9 ( 2. p00 p=0.0 1.1 1.021.3 1021 1. 13 1.3 1.02-1. x23=10. x231 =10. [x2(3)=10 7. 321 3.3215.8 33215 33 158 15.8 3.32-15. 0.0 p<0.0 x24=59. x245 =59. [x2(4)=59 95%CI1.0011.00 95CI100110 95%CI:1.001-1.0 06 0.6 0.5720.65 057206 057 0.5 065 0.572-0.65 5,750/11, 575011 312411 3,124/11, p0 p=0. 1.021. 1.02-1 x23=10 =10 [x2(3)=1 3.3215. 3321 15. 3.32-15 0. p<0. x24=59 =59 [x2(4)=5 95%CI1.0011.0 95CI10011 95%CI:1.001-1. 0.5720.6 05720 05 0.572-0.6 5,750/11 57501 31241 3,124/11 p=0 1.021 1.02- x23=1 =1 [x2(3)= 3.3215 3.32-1 p<0 x24=5 =5 [x2(4)= 95%CI1.0011. 95CI1001 95%CI:1.001-1 0.5720. 0.572-0. 5,750/1 5750 3124 3,124/1 p= x23= = [x2(3) 3.321 3.32- p< x24= [x2(4) 95%CI1.0011 95CI100 95%CI:1.001- 0.5720 0.572-0 5,750/ 575 312 3,124/ [x2(3 [x2(4 95%CI1.001 95CI10 95%CI:1.001 0.572- 5,750 31 3,124 [x2( 95%CI1.00 95CI1 95%CI:1.00 5,75 3,12 [x2 95%CI1.0 95%CI:1.0 5,7 3,1 [x 95%CI1. 95%CI:1. 5, 95%CI1 95%CI:1

Abstract Objective This study aims to explore the regulatory mechanism of long noncoding RNA X inactive specific transcript (lncRNA XIST) in the odontogenic differentiation of human dental pulp stem cells (hDPSCs). hDPSCs were obtained from freshly extracted third molars and identified by flow cytometry. Methodology Odontogenic differentiation was induced in mineralized culture medium, and hDPSCs were infected with shRNA lentivirus targeting XIST or fused in sarcoma (FUS), followed by detection of alkaline phoshpatase (ALP) activity, alizarin red staining of mineralized nodules, Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) quantification of XIST expression, and Western blot analysis of FUS, ZBTB16, and odontogenic differentiation markers (DSPP and DMP1). IF-FISH was performed to detect the cellular localization of XIST and FUS. RIP assay validated the XIST and FUS binding. ZBTB16 mRNA stability was tested after actinomycin D treatment. hDPSCs were infected with oe-ZBTB16 lentivirus and further treated with sh-XIST for a combined experiment. Results LncRNA XIST was highly expressed in hDPSCs with odontogenic differentiation. Downregulation of XIST or FUS weakened the ALP activity of hDPSCs, reduced mineralized nodules, diminished DSPP and DMP1 expressions. XIST binds to FUS to stabilize ZBTB16 mRNA and promote ZBTB16 expression. ZBTB16 overexpression partially reversed the inhibitory effect of XIST silencing on odontogenic differentiation of hDPSCs. Conclusion In conclusion, XIST stabilizes ZBTB16 mRNA and promotes ZBTB16 expression by binding to FUS, thereby facilitating the odontogenic differentiation of hDPSCs. lncRNA . (hDPSCs) cytometry medium , (FUS) (ALP nodules RealTime Real Time qRTPCR qRT (qRT-PCR ZBTB DMP1. DMP DMP1) IFFISH IF FISH ZBTB1 treatment oeZBTB16 oeZBTB oe oe-ZBTB1 shXIST sh experiment expressions conclusion (hDPSCs (FUS oeZBTB1 oe-ZBTB

SUMMARY OBJECTIVE: The aim of this study was to evaluate the success rate and predictors of labor induction using vaginal misoprostol in a low-risk pregnant women population. METHODS: A prospective cohort study was carried out with 196 pregnant women. Groups 2 and 4 of the Robson Classification admitted for induction of labor with vaginal misoprostol (25 μg tablets every 6 h, up to 4 tablets, for a maximum of 24 h). The success of labor induction was considered the achievement of vaginal delivery. Binary logistic regression was used to determine the best predictors of successful induction of labor with vaginal misoprostol. RESULTS: Of all the pregnant women analyzed, 140 (71.4%) were successful and 56 (28.6%) were unsuccessful. Pregnant women who achieved successful induction had a higher number of pregnancies (1.69 vs. 1.36, p=0.023), a higher number of deliveries (0.57 vs. 0.19, p<0.001), a higher Bishop score (2.0 vs. 1.38, p=0.002), and lower misoprostol 25 μg tablets (2.18 vs. 2.57, p=0.031). No previous deliveries [x2(1)=3.14, odds ratio (OR): 0.24, 95% confidence interval (CI): 0.10–0.57, R2 Nagelkerke: 0.91, p=0.001] and the presence of one previous delivery [x2(1)=6.0, OR: 3.40, 95% CI: 1.13–10.16, R2 Nagelkerke: 0.043, p=0.029] were significant predictors of successful induction of labor with vaginal misoprostol. CONCLUSION: A high rate of labor induction success using vaginal misoprostol in a low-risk population was observed, mainly in multiparous and with gestational age>41 weeks. No previous delivery decreased the success of labor induction, while one previous delivery increased the success of labor induction. OBJECTIVE lowrisk low risk METHODS 19 (2 h h. . h) RESULTS analyzed 14 71.4% 714 71 (71.4% 5 28.6% 286 28 (28.6% unsuccessful 1.69 169 1 69 (1.6 vs 136 36 1.36 p=0.023, p0023 p p=0.023 , 0 023 p=0.023) 0.57 057 57 (0.5 019 0.19 p<0.001, p0001 p<0.001 001 p<0.001) 2.0 20 (2. 138 38 1.38 p=0.002, p0002 p=0.002 002 p=0.002) 2.18 218 18 (2.1 257 2.57 p=0.031. p0031 p=0.031 031 p=0.031) x21=3.14, x21314 x x2 =3.14, 3 [x2(1)=3.14 OR (OR) 024 0.24 95 CI (CI) 010057 10 0.10–0.57 R Nagelkerke 091 91 0.91 p=0.001 x21=6.0, x2160 =6.0, [x2(1)=6.0 340 40 3.40 1131016 13 16 1.13–10.16 0043 043 0.043 p=0.029 p0029 029 CONCLUSION observed age41 age 41 age>4 weeks ( 71.4 7 (71.4 28.6 (28.6 1.6 (1. 1.3 p002 p=0.02 02 0.5 05 (0. 01 0.1 p000 p<0.00 00 2. p=0.00 2.1 21 2.5 p003 p=0.03 03 x21 x21=3.14 x2131 314 =3.14 [x2(1)=3.1 (OR 0.2 9 (CI 01005 0.10–0.5 09 0.9 x21=6.0 x216 60 =6.0 [x2(1)=6. 34 3.4 113101 1.13–10.1 004 04 0.04 age4 age> 71. (71. 28. (28. 1. (1 p00 p=0.0 0. (0 p<0.0 x21=3.1 x213 31 =3.1 [x2(1)=3. 0100 0.10–0. x21=6. =6. [x2(1)=6 3. 11310 1.13–10. 0.0 (71 (28 p0 p=0. p<0. x21=3. =3. [x2(1)=3 010 0.10–0 x21=6 =6 [x2(1)= 1131 1.13–10 (7 p=0 p<0 x21=3 =3 0.10– x21= = [x2(1) 113 1.13–1 p= p< 0.10 [x2(1 11 1.13– [x2( 1.13 [x2 1.1 [x

ABSTRACT Considering the importance of backyard broiler production in Zambia, this study aimed to characterize this practice in the Serenje district (Zambia). A structured questionnaire was administered to 55 households. Results revealed a male predominance (56.36%) in broiler ownership, with most (69.09%) falling within the 30 to 50 years age group. Most (72.73%) had received at least secondary education. Notably, broiler producers primarily depended on farming (41.82%) and formal employment (32.73%) for income. Marketing channels predominantly involved direct sales to consumers (78.18%), minimizing intermediary involvement. The study highlighted the seasonal demand for broilers. Broiler consumption was common (90.91%) among producers, with most (85.45%) consuming broiler meat up to twice a week. Biosecurity practices varied, and mortality management predominantly involved burning or burial (67.27%). While most (96.36%) farmers practiced vaccinations, disease outbreaks persisted (92.73%), with limited consultation of veterinarians (67.27%). Access to information on broiler production primarily relied on agricultural agencies and organizations (49.09%). Common challenges included feed costs (94.55%), diseases (92.73%), and price fluctuations (87.27%). This study provides valuable insights into the dynamics of backyard broiler production in an urban setting, shedding light on its potential impacts on public health and the overall poultry industry. Zambia Zambia. . (Zambia) 5 households 56.36% 5636 56 36 (56.36% ownership 69.09% 6909 69 09 (69.09% 3 group 72.73% 7273 72 73 (72.73% education Notably 41.82% 4182 41 82 (41.82% 32.73% 3273 32 (32.73% income 78.18%, 7818 78.18% , 78 18 (78.18%) involvement broilers 90.91% 9091 90 91 (90.91% 85.45% 8545 85 45 (85.45% week varied 67.27%. 6727 67.27% 67 27 (67.27%) 96.36% 9636 96 (96.36% vaccinations 92.73%, 9273 92.73% 92 (92.73%) 49.09%. 4909 49.09% 49 (49.09%) 94.55%, 9455 94.55% 94 (94.55%) 87.27%. 8727 87.27% 87 (87.27%) setting industry (Zambia 56.36 563 (56.36 69.09 690 6 0 (69.09 72.73 727 7 (72.73 41.82 418 4 8 (41.82 32.73 327 (32.73 781 78.18 1 (78.18% 90.91 909 9 (90.91 85.45 854 (85.45 672 67.27 2 (67.27% 96.36 963 (96.36 927 92.73 (92.73% 490 49.09 (49.09% 945 94.55 (94.55% 872 87.27 (87.27% 56.3 (56.3 69.0 (69.0 72.7 (72.7 41.8 (41.8 32.7 (32.7 78.1 (78.18 90.9 (90.9 85.4 (85.4 67.2 (67.27 96.3 (96.3 92.7 (92.73 49.0 (49.09 94.5 (94.55 87.2 (87.27 56. (56. 69. (69. 72. (72. 41. (41. 32. (32. 78. (78.1 90. (90. 85. (85. 67. (67.2 96. (96. 92. (92.7 49. (49.0 94. (94.5 87. (87.2 (56 (69 (72 (41 (32 (78. (90 (85 (67. (96 (92. (49. (94. (87. (5 (6 (7 (4 (3 (78 (9 (8 (67 (92 (49 (94 (87 (

Abstract High entropy alloy is a multi-component alloy material with equal or near atomic number, which has excellent wear resistance and corrosion resistance. In this paper, AlxFeCoCrNiMn (x = 0,0.5,1.0,1.5) high entropy alloy cladding layer was prepared by laser cladding on Q345 steel plate. The phase structure, microstructure, hardness and wear resistance of the cladding layer were studied. The results show that the cladding layer of AlxFeCoCrNiMn alloy has good metallurgical bonding ability with Q345 steel. The cladding area is mainly columnar and equiaxed. The cladding layer of AlxFeCoCrNiMn alloy is mainly composed of BCC + FCC solid solution phase, which is caused by the addition of Al element under the high entropy effect. Al element promotes the formation of BCC phase structure. With the increase of Al element, the hardness of cladding layer increases. The hardness of A1.0 cladding layer is 670HV, which is close to three times of Q345 steel. When Al (x = 1.0), the hardness of the cladding layer is the highest, and it also shows better wear resistance with lower friction and wear loss. multicomponent multi component number paper x 0,0.5,1.0,1.5 0051015 0 5 1 Q Q34 plate structure microstructure studied equiaxed effect increases A10 A A1 A1. 670HV HV 1.0, 10 1.0 , 1.0) highest loss 0,0.5,1.0,1. 005101 Q3 1. 0,0.5,1.0,1 00510 0,0.5,1.0, 0051 0,0.5,1.0 005 0,0.5,1. 00 0,0.5,1 0,0.5, 0,0.5 0,0. 0,0 0,

Abstract In this study, we incorporated gallic acid (GA) and salicylic acid (SA) onto chitosan (CS) using free radical grafting initiated by hydrogen peroxide/Vitamin C (H2O2/Vc) redox system. We characterized the structural properties of the GA (CS-GA) and SA (CS-SA) derivatives using UV–vis absorption, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), nuclear magnetic resonance (NMR), and thermal stability analysis. We proved that these phenolic acids were successfully grafted onto the molecular skeleton of CS. We investigated the antioxidant and antibacterial properties of CS-GA and CS-SA and found that they had significantly higher antioxidant and antibacterial properties than native CS. Furthermore, the antioxidant ability increased with the increase in grafting ratios. Finally, in vivo tests showed that CS-GA could maintain the firmness and the content of soluble solids and titratable acids in Saimaiti apricots at cool storage. The storage period was prolonged by inhibiting the respiratory intensity of apricots. Our results suggest that CS-GA can be potentially used as an edible coating material to preserve apricots. study (GA (SA CS (CS peroxideVitamin peroxide Vitamin H2O2/Vc H2O2Vc HOVc H2O2 Vc H O (H2O2/Vc system CSGA (CS-GA CSSA (CS-SA UVvis UV vis absorption FTIR, FTIR , (FTIR) Xray X ray XRD, XRD (XRD) NMR, NMR (NMR) analysis Furthermore ratios Finally HO H2O (FTIR (XRD (NMR

Abstract Introduction: The objective of this study is to investigate the protective effect of kaempferol against ischemia/reperfusion (IR) injury and the underlying molecular mechanisms. Methods: H9C2 cells were pretreated with kaempferol for 24 hours and further insulted with IR injury. Cell vitality, reactive oxygen species (ROS) level, glutathione (GSH) level, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, and sirtuin-3 (SIRT3), B-cell lymphoma 2 (Bcl2), and Bcl2-associated X protein (Bax) expressions were evaluated. Moreover, short interfering ribonucleic acid targeting SIRT3 was used to investigate the role of SIRT3 against IR mediated by kaempferol in vitro. IR mice models were also established to confirm the protective effects of kaempferol on IR in vivo. Results: After IR injury, H9C2 cells vitality was reduced, ROS levels, NADPH oxidase activity, and Bax expressions were increased, and GSH levels and Bcl2 expressions were decreased. After kaempferol pretreatment, the vitality of H9C2 cells was increased. The levels of ROS, NADPH oxidase activity, and Bax expression were decreased. In addition, levels of GSH and Bcl2 expression were enhanced. Furthermore, silencing SIRT3 attenuated the protective effect mediated by kaempferol, with increased ROS levels, NADPH oxidase activity, and Bax expression, along with reduced GSH level and Bcl2 expression. In vivo IR model showed that kaempferol could preserve IR-damaged cardiac function. Conclusion: Kaempferol has the capability of attenuating H9C2 cells IR injury through activating SIRT3 to inhibit oxidative stress.

SUMMARY: To evaluate the skeletal, dento-alveolar and soft tissue morphology changes after maxillary molar distalization by clear aligner therapy and identify the significant efficacy of molar distalization,18 patients in conformity with the inclusion criteria were selected. Pre- and post-treatment Cone Beam Computed Tomography (CBCT) were examined to measure the angular and linear parameters. All subjects were completed non-extraction clear aligner treatment by distalizing molars. A paired-t test and independent-samples t-test were performed to observe the difference between before and after treatment and the difference between the first molar and second molar respectively. P-values <0.05 were considered statistically significant. Predicted movement rate was calculated by the formula: (actual movement(mm)/planned movement(mm)) x100%. Most variables of pre- and post-treatment showed no statistical difference(P<0.05), excepting SNA angle (P<0.05) and Upper lip/E-line linear (P<0.01) due to incisor retraction. The first and second molar revealed a translation movement without significant tipping and vertical movement. Clear aligners provided a high predictability (83.44 %) of distalization the maxillary first molar, and 85.14 % of the maxillary second molar. Clear aligners can effectively achieve distal displacement of molars.

RESUMEN: Se seleccionaron 18 pacientes, de acuerdo con los criterios de inclusión, para evaluar los cambios en la morfología esquelética, dentoalveolar y de los tejidos blandos después de la distalización de los molares maxilares, mediante la terapia con alineadores transparentes e así identificar la significativa eficacia de la distalización de los molares. Se examinó a través de tomografía computarizada de haz cónico (CBCT) antes y después del tratamiento para medir los parámetros angulares y lineales. Todos los sujetos completaron el tratamiento con alineadores transparentes sin extracción mediante la distalización de los molares. Se realizó una prueba t pareada y una prueba t de muestras independientes para observar la diferencia entre antes y después del tratamiento y la diferencia entre el primer molar y el segundo molar, respectivamente. Los valores de p<0,05 se consideraron estadísticamente significativos. La tasa de movimiento prevista se calculó mediante la fórmula: (movimiento real (mm)/movimiento planificado (mm)) x 100 %. La mayoría de las variables de pre y postratamiento no mostraron diferencia estadística (P<0,05), excepto el ángulo SNA (P<0,05) y el labio superior/línea E lineal (P<0,01) debido a la retracción del incisivo. El primer y segundo molar revelaron un movimiento de traslación sin inclinación significativa y movimiento vertical. Los alineadores transparentes proporcionaron una alta previsibilidad (83,44 %) de la distalización del primer molar superior y del 85,14 % del segundo molar superior. Los alineadores transparentes pueden lograr efectivamente el desplazamiento distal de los molares.

ABSTRACT Abdominal cocoon syndrome is a rare cause of intestinal obstruction, which is difficult to diag- nose preoperatively. We here report a case of abdominal cocoon. A 47-year-old male patient was referred to the general surgery department with complaints of abdominal pain, distension, nausea and vomiting for 1 day. An abdominal computed tomography examination detected the dilated small intestinal loops clustered in the abdomen and surrounded by a sac-like membrane. During the exploratory surgery, a capsular structure was identified in the lower quadrant with a regular surface that was solid fibrous in nature. The combination of physical examination, imaging signs and medical history may be helpful in the diagnosis.

SUMMARY: This study aimed to investigate the sagittal spinal-pelvic morphological changes, as well as the relationship between pelvic anatomical changes and the spinal-pelvic plane in patients with adolescent idiopathic scoliosis (AIS), in order to provide guidelines for orthopedic surgery in AIS. X-ray data were collected for retrospective analysis from 30 patients diagnosed as AIS in the Departments of Radiology at the Second Affiliated Hospital of Inner Mongolia Medical University and the Inner Mongolia International Mongolian Medical Hospital from April 2014 to November 2018, along with 30 normal adolescents as control. Pelvic parameters, including pelvic incidence (PI), pelvic tilt (PT), and sacral slope (SS), a spinal parameter, lumbar lordosis (LL), and anatomical parameters, including sacral width (SW) and femoral head- sacrum distance (FH-S), were measured. The spinal-pelvic parameters were compared between AIS patients and normal controls and also between male and female AIS patients. Pearson correlation was performed to analyze correlation between spinal-pelvic parameters and between spinal-pelvic parameters and anatomical parameters in both AIS patients and normal controls. PT was significantly lower in AIS patients than in normal controls (P < 0.05), whereas no significant difference was found in the other spinal-pelvic parameters, i. e. , LL, PI, and SS. There was a significant difference in PT between sexes in AIS patients. SS was significantly correlated with LL in EIA patients (P < 0.05, r > 0.5). SS was significantly correlated with LL and PI, and PT with LL, PI, and SS in normal controls (all P < 0.05), and there was no significant correlation between the other sagittal spinal-pelvic parameters (P > 0.05). FH-S was significantly correlated with LL, PI, SS, and PT in AIS patients (all P < 0.05). AIS affects some of the sagittal spinalpelvic parameters and anatomical parameters. In AIS, there is a significant correlation between the spinal-pelvic parameters, and the anatomical parameter is significantly correlated with multiple spinal-pelvic parameters.

RESUMEN: Este estudio tuvo como objetivo investigar los cambios morfológicos sagitales de la columna vertebral-pélvica, así como la relación entre los cambios anatómicos pélvicos y el plano espinal-pélvico en pacientes con escoliosis idiopática adolescente (EIA), con el fin de proporcionar pautas para la cirugía ortopédica en AIS. Se obtuvieron los datos de rayos X para el análisis retrospectivo de 30 pacientes diagnosticados como EIA en los Departamentos de Radiología del hospital Second Affiliated Hospital of Inner Mongolia Medical University y el hospital Inner Mongolia International Mongolian Medical Hospital, desde abril de 2014 hasta noviembre del 2018, junto con 30 adolescentes normales como control. Se midieron los parámetros pélvicos, que incluyeron incidencia pélvica (IP), inclinación pélvica (P) y pendiente sacra (PS), un parámetro espinal, lordosis lumbar (LL) y parámetros anatómicos, que incluyeron el ancho sacro (AS) y la distancia del sacro cabeza femoral (FH-S). Los parámetros espinalpélvicos se compararon entre los pacientes con EIA y los controles normales, como también entre pacientes con EIA masculinos y femeninos. La correlación de Pearson se realizó para analizar la correlación entre los parámetros espinal-pélvicos y entre los parámetros espinal-pélvicos y los parámetros anatómicos tanto en pacientes con EIA como en controles normales. PT fue significativamente menor en pacientes con EIA que en los controles normales (P <0,05), mientras que no se encontraron diferencias significativas en los otros parámetros espinal-pélvicos, i. mi. , LL, PI y SS. Hubo una diferencia significativa en PT en pacientes de ambos sexos con EIA. SS se correlacionó significativamente con LL en pacientes con EIA (P <0,05, r> 0,5). SS se correlacionó significativamente con LL y PI, y PT con LL, PI y SS en controles normales (todos P <0,05), y no hubo correlación significativa entre los otros parámetros sagitales de la columna vertebral-pélvica (P> 0,05) FH-S se correlacionó significativamente con LL, PI, SS y PT en pacientes con EIA (todos P <0,05). EIA afecta algunos de los parámetros sagitales de la columna vertebral-pélvica y los parámetros anatómicos. En EIA, existe una correlación significativa entre los parámetros espinal-pélvicos, y el parámetro anatómico se correlaciona significativamente con múltiples parámetros espinales-pélvicos.

ABSTRACT Expression of housekeeping genes is relatively constant in different tissues and cells by RT-qPCR analysis. Housekeeping genes (HGs) are usually utilized as the reference to evaluate and compare mRNA expression abundances of target genes in different cells or tissues sampled. However, the expression stabilities of different HGs in diverse samples may appear divergence. Currently, there is no exact reference data of HGs in hen ovarian follicular tissues during egg-laying period available yet. In this study, we detected the expression of 18SrRNA, ACTB, HOXC8, GAPDH, alpha-A, and alpha-D mRNA in the varied-size ovarian follicles (1-8 mm in diameter and F5), hearts, livers, spleens, lungs, and breast muscles of the laying hens by RT-qPCR, to analyze the results via Ct value, geNorm, Normfinder, and Bestkeeper. The data showed that the expression levels of 18SrRNA, alpha-A, and alpha-D transcripts were more significantly stable than the other three genes for normalizing mRNA expression in the hen ovarian follicles examination. Moreover, alpha-D, 18SrRNA, and alpha-A were also most suitable for the expression normalization in the tissues of the heart, liver, spleen, lung and breast muscle. In contrast, 18SrRNA has the most stable mRNA expression levels in all tissues sampled, so it can serve as an excellent inner control for the evaluation of the transcription levels in chickens. It is a remarkable fact that HOXC8 as a candidate reference should be avoided. Our study establishes a set of stably expressed candidate inner references in the hen ovarian follicles and several tissues, it firstly provided an exact data for validation of the inner references in normalizing transcription levels of a target gene in chickens.

Abstract This study investigated the protective effect of crocetin from Crocus sativus L. on myocardial ischemia-reperfusion injury (MIRI) in rats. Sixty SD rats were randomly divided into sham-operated, model, and low-, medium- and high-dose crocetin groups. Later 3 groups were intragastrically administrated with 10, 20 and 40 mg/kg crocetin from Crocus sativus L., respectively, for 1 week. On the 8th day, the MIRI model was established in the later 4 groups. The blood biochemical indexes, hemodynamic indexes, myocardial infarct size, myocardial antioxidant indexes and myocardial expressions of B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), serine/threonine kinase (Akt) and phosphorylated Akt (p-Akt) protein were determined. Results showed that, compared with model group, in high-dose crocetin group the left ventricular systolic pressure, +dp/dtmax and -dp/dtmax, myocardial superoxide dismutase and glutathione peroxidase levels, and myocardial Bcl-2/Bax ratio and p-Akt/Akt ratio were significantly increased, and the left ventricular end diastolic pressure, myocardial infarct size, serum lactate dehydrogenase, creatine kinase-MB, cardiac troponin I, tumor necrosis factor-α, interleukin-1β, interleukin-6 and malondialdehyde levels were significantly decreased (all P < 0.05). In conclusion, crocetin from Crocus sativus L. has protective effects on MIRI in rats.

RESUMO OBJETIVO O câncer de pulmão é a principal causa de mortes relacionadas ao câncer. Para reduzir a mortalidade por câncer de pulmão e encontrar câncer de pulmão em um estágio inicial, é necessária uma triagem por tomografia de baixa dose. Uma meta-análise foi emitida para testemunhar se a triagem poderia reduzir a mortalidade por câncer de pulmão e investigar o melhor programa de triagem. MÉTODOS Pesquisamos PubMed, Web of Science, biblioteca Cochrane, ScienceDirect e relevante banco de dados chinês. Ensaios clínicos controlados aleatórios, em que os participantes eram fumantes com mais de 49 anos (tabagismo >15 anos ou parar de fumar 10 ou 15 anos atrás) foram incluídos. RESULTADOS Nove estudos RCT preencheram os critérios. O rastreamento de LDCT pôde encontrar mais cânceres de pulmão (RR=1,58, IC 95%=1,25-1,99, P<0,001) e mais cânceres de estágio I do pulmão (RR=3,45, IC 95%=2,08-5,72, P<0,001) em comparação com raio X do tórax ou nenhum grupo de triagem. Ele indicou uma redução estatisticamente significativa na mortalidade específica do câncer de pulmão (RR=0,84, IC 95%=0,75-0,95, P=0,004), mas sem uma redução estatisticamente significativa na mortalidade por todas as causas (RR=1,26, IC 95%=0,89-1,78, P=0,193). Anualmente, o rastreamento de LDCT foi sensível em encontrar mais cânceres de pulmão. CONCLUSÕES A triagem de TC de baixa dose é eficaz para encontrar mais cânceres de pulmão e diminuir as mortes por câncer de pulmão. Para encontrar mais cânceres de pulmão em estágio inicial, a triagem anual de tomografia de baixa dose pode ser melhor do que a triagem bianual.

SUMMARY OBJECTIVE Lung cancer is the leading cause of cancer-related death. To reduce lung cancer mortality and detect lung cancer in early stages, low dose CT screening is required. A meta-analysis was conducted to verify whether screening could reduce lung cancer mortality and to determine the optimal screening program. METHODS We searched PubMed, Web of Science, Cochrane library, ScienceDirect, and relevant Chinese databases. Randomized controlled trial studies with participants that were smokers older than 49 years (smoking >15 years or quit smoking 10 or 15 years ago) were included. RESULTS Nine RCT studies met the criteria. LDCT screening could find more lung cancer cases (RR=1.58, 95%CI=1.25-1.99, P<0.001) and more stage I lung cancers (RR=3.45, 95%CI=2.08-5.72, P<0.001) compared to chest-X ray or the no screening group. This indicated a statistically significant reduction in lung-cancer-specific mortality (RR=0.84, 95%CI=0.75-0.95, P=0.004), but without a statistically reduction in mortality due to all causes (RR=1.26, 95%CI=0.89-1.78, P=0.193). Annually, LDCT screening was sensitive in finding more lung cancers. CONCLUSIONS Low-dose CT screening is effective in finding more lung cancer cases and decreasing the deaths from lung cancer. Annual low-dose CT screening may be better than a biennial screening to detect more early-stage lung cancer cases.

RESUMO OBJETIVOS: A perfusão por TC objetiva (CTP) é uma técnica de imagem que pode ser usada para avaliar as alterações na microcirculação de tecidos tumorais. Nosso estudo teve como objetivo investigar o papel da CTP na predição de metástases em linfonodos mediastinais. MÉTODOS: Dados clínicos de 58 pacientes que receberam ressecção cirúrgica de câncer de pulmão e dissecção de linfonodos em nosso hospital de junho de 2012 a dezembro de 2014 foram coletados. Os pacientes foram divididos em grupo positivo para metástase linfonodal e grupo negativo para metástase linfonodal. Parâmetros de CTP incluindo pico de intensidade de realce (PEI) e valor de perfusão (PV), bem como volume de sangue (BV), foram comparados entre os dois grupos. A curva característica de operação do receptor (ROC) foi usada para predizer metástase linfonodal mediastinal. RESULTADOS: PV do grupo de linfonodos metastáticos positivos foi significativamente maior do que o grupo negativo de linfonodos metastáticos (p<0,001). A análise da curva ROC mostrou que a PV pode ser usada como um índice para predizer a metástase linfonodal mediastinal do câncer de pulmão. A sensibilidade e a especificidade da VP maior que 7,5ml · min-1 · ml-1 na predição de metástase linfonodal de câncer de pulmão foram de 78,3% e 91,4%, respectivamente. CONCLUSÃO: A VP de perfusão por TC de baixa dose pode ser usada como um índice para a predição de metástase linfonodal mediastinal de câncer de pulmão.

SUMMARY OBJECTIVE: CT perfusion (CTP) is an imaging technique that can be used to evaluate the changes in the microcirculation of tumor tissues. Our study aimed to investigate the role of CTP in predicting mediastinal lymph node metastasis. METHODS: Clinical data of 58 patients who received surgical resection of lung cancer and lymph node dissection in our hospital from June 2012 to December 2014 were collected. Patients were divided into a positive lymph node metastasis group and a negative lymph node metastasis group. Parameters of CTP, including peak enhancement intensity (PEI), perfusion value (PV), as well as blood volume (BV), were compared between the two groups. The receiver-operating characteristic (ROC) curve was used to predict mediastinal lymph node metastasis. RESULTS: The PV of the positive lymph node metastasis group was significantly higher than that of the negative group (p < 0.001). The ROC curve analysis showed that PV can be used as an index to predict mediastinal lymph node metastasis of lung cancer. The sensitivity and specificity of a PV greater than 7.5ml·min-1·ml-1 in predicting lymph node metastasis of lung cancer were 78.3 % and 91.4 %, respectively. CONCLUSION: The PV of low dose CT perfusion can be used as an index for predicting mediastinal lymph node metastasis of lung cancer.