RESUMEN Como medida de control contra las malezas, el uso de herbicidas es una alternativa efectiva y asequible. Sin embargo, no hay productos recomendados para el cultivo de frijol caupí en Brasil, por lo que es necesario buscar soluciones alternativas. El objetivo de este estudio fue evaluar el efecto de herbicidas aplicados en pre- y post-emergencia sobre la nodulación y productividad del frijol caupí bajo las condiciones de la sabana amazónica. Se realizaron dos experimentos en bloques al azar con cuatro repeticiones, utilizando el cultivar de frijol caupí BRS Aracê sometido a los herbicidas pre-emergentes: Metribuzina, Sulfentrazona, S-metolaclor, Pendimetalina, Oxadiazón, Alaclor, Metribuzina + Pendimetalina, Metribuzina + Alaclor y Quizalofop-p-etil, Bentazona, Fomesafen, Imazetapir, Imazamox + Bentazona, Quizalofop-p-etil + Imazetapir, Quizalofop-p-etil + Imazamox y Quizalofop-p-etil + Bentazona, y herbicidas post-emergentes: Quizalofop-p-etil, Bentazona, Fomesafen, Imazetapir, Imazamox + Bentazona, Quizalofop-p-etil + Imazetapir, Quizalofop-p-etil + Imazamox, Quizalofop-p-etil + Bentazona. Se evaluaron el número de nódulos por planta, la masa seca de nódulos, la masa seca de raíces y el rendimiento de grano. Según los resultados obtenidos en este estudio, el manejo de malezas en pre o post-emer-gencia de acuerdo con el herbicida utilizado afecta la nodulación y el rendimiento de grano de frijol caupí bajo condiciones de la sabana amazónica. Los herbicidas Metribuzina en pre-emergen-cia, y Fomesafen y la mezcla de Quizalofop-p-etil + Imazetapir en post-emergencia no se recomiendan para el control de malezas en frijol caupí. La aplicación de Oxadiazón, Alaclor y Pendimetalina en la pre-emergencia puede considerarse interesante porque no inhiben el desarrollo del sistema radicular o la nodulación de frijol caupí lo que proporciona un mayor rendimiento de grano. En lo relacionado con las estrategias de control de malezas posteriores a la emergencia, la aplicación del herbicida Imazetapir y la combinación de los herbicidas Quizalofop-p-etil + Imazamox, Quizalofop-p-etil + Bentazona e Imazamox + Bentazona permiten niveles satisfactorios de rendimiento de grano, desarrollo del sistema de raíces y nodulación de frijol caupí.

Abstract As a control measure against weeds, the use of herbicides is an effective and inexpensive alternative. However, there are no products recommended for the cultivation of cowpea in Brazil, making it necessary to search for alternative solutions. The objective of this study was to evaluate the effect of herbicides applied in the pre- and post-emergence on cowpea nodulation and production under conditions of the Amazonian savannah. Two experiments were carried out in a randomized block design with four replicates, using the cowpea cultivar BRS Aracê subjected to the pre-emergence herbicides: Metribuzin, Sulfentrazone, S-metolachlor, Pendimethalin, Oxadiazon, Alachlor, Metribuzin + Pendimethalin, Metribuzin + Alachlor and Quizalofop-p-ethyl, Bentazon, Fomesafen, Imazethapyr, Imazamox + Bentazon, Quizalofop-p-ethyl + Imazethapyr, Quizalofop-p-ethyl + Ima-zamox and Quizalofop-p-ethyl + Bentazon, and post-emergence herbicides: Quizalofop-p-ethyl, Bentazon, Fomesafen, Imazetha-pyr, Imazamox + Bentazon, Quizalofop-p-ethyl + Imazethapyr, Quizalofop-p-ethyl + Imazamox, and Quizalofop-p-ethyl + Bentazon. The number of nodules in each plant, the dry matter of nodules, dry matter of roots and the grain yield were evaluated. According to the results obtained, the management of weeds in pre- or post-emergence according to the herbicide used affects the nodulation and productivity of cowpea under the conditions of the Amazonian savannah. The herbicides Metribuzin in pre-emergence, and Fomesafen and the mixture of Quizalofop-p-ethyl + Imazethapyr in post-emergence are not recommended for weed control in cowpea. The application of Oxadiazon, Alachlor, and Pendimethalin in pre-emergence can be considered interesting because they do not inhibit the development of the root system or the nodulation of cowpea which provides a greater grain yield. Regarding weed control strategies at post-emergence, the application of the herbicide Imazethapyr and the combination of the herbicides quizalofop-p-ethyl + imazamox, Quizalofop-p-ethyl + Bentazon and Imazamox + Bentazon allow satisfactory levels of grain yield, root system development and nodulation of cowpea.

ABSTRACT Although there are already available pineapple cultivars resistant to fusariosis, Brazilian production is based on the cultivar Pérola, susceptible to fusariosis. Studies with these resistant cultivars should prioritize the fulfillment of nutritional requirements, so that they express their productive potential and become viable options for producers. The objective of this study was to study the effects of foliar fertilizer compositions on the growth and yield of five micropropagated pineapple cultivars. The study was started in a greenhouse and at 210 days after transplanting, the seedlings were transferred to field conditions. The experimental design was in randomized blocks, in subdivided plots with three factors and four replications. The combination of the five pineapple cultivars (BRS Ajubá, IAC Fantástico, BRS Imperial, Pérola and BRS Vitória) and three leaf fertilizer compositions (NK, NPK and NPK + micronutrients) were randomized in plots and six evaluation periods consisted of subplots. Agronomic attributes were analyzed at the time of evaluation. The results obtained were subjected to analysis of variance by the F test. The Scott-Knott test was used for comparisons between cultivars and fertilization, and regression for the seasons, using the SISVAR program. Regardless of fertilization, cv. IAC Fantástico stood out in the majority of the evaluated characters, being superior to cv. Pérola, considered standard. The NK formula shows to be the best option for use as leaf fertilizer in the pineapple crop. The cultivar IAC Fantástico has good vegetative and productive characteristics, having the potential to be a viable option for cultivation in the state of Roraima.

RESUMO Embora já se disponha de cultivares de abacaxizeiro resistentes à fusariose, a produção brasileira é baseada na cultivar Pérola, susceptível à fusariose. Estudos com essas cultivares resistentes devem priorizar o atendimento das exigências nutricionais, para que elas expressem seu potencial produtivo e se tornem opções viáveis para os produtores. Objetivou-se estudar os efeitos de composições de adubo foliar no crescimento e produção de cinco cultivares de abacaxizeiro micropropagadas. O estudo foi iniciado em casa de vegetação e aos 210 dias após o transplantio, as mudas foram transferidas para o campo. O delineamento experimental foi em blocos casualizados, em parcelas subdivididas com três fatores e quatro repetições. A combinação das cinco cultivares de abacaxizeiro (BRS Ajubá, IAC Fantástico, BRS Imperial, Pérola e BRS Vitória) e três composições de adubo foliar (NK, NPK e NPK + micronutrientes) foram aleatorizadas nas parcelas e seis épocas de avaliação, nas subparcelas. Atributos agronômicos foram analisados nas épocas de avaliação. Os resultados obtidos foram submetidos à análise de variância pelo teste F. Foi empregado o teste de Scott-Knott para as comparações entre cultivares e adubação, e de regressão para as épocas, empregando-se o programa SISVAR. Independente da adubação, a cv. IAC Fantástico se destacou na maioria dos caracteres avaliados, sendo superior a cv. Pérola, considerada padrão. A fórmula NK mostra ser a melhor opção para utilização como adubo foliar na cultura do abacaxizeiro. A cultivar IAC Fantástico apresenta boas características vegetativas e produtivas, tendo potencial para ser uma opção viável para cultivo no estado de Roraima.

The essential oil of the leaves of Eugenia sulcata, in the Myrtaceae family, has a demonstrated antihypertensive effect, but its effects on heart muscle and its toxicity have not yet been elucidated. Little chemical or biological data are available for E. sulcata, whether emphasizing the beneficial effects or the pharmacological security of this species. This study aims to evaluate myocardial contractility and to analyze angiotensin converting enzyme (ACE) and myosin ATPase activities associated with use of this essential oil. In addition, we evaluated the immunotoxicity of E. sulcata essential oil. Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) were treated daily for 30 days (10 mg/kg of oil) to evaluate the isometric force of the papillary muscle, ACE measured by fluorimetry, and myosin ATPase activities by inorganic phosphate. Lymphocyte cultures were used to evaluate cytotoxicity, DNA damage, and mutagenicity of the essential oil. The results demonstrate that the treatment did not change the cardiac contraction force and did not alter the functioning of the sarcoplasmic reticulum, extrusion of the membrane calcium, or modify the membrane calcium channels or β-adrenergic receptor activity. Tetanic contractions were potentiated in the SHR animals. Myosin ATPase activity was also increased in the SHR animals. Cardiac ACE activity was reduced in both animal strains, and the serum ACE was reduced only in the SHR animals. The essential oil did not cause cytotoxicity or mutagenicity and presented low DNA damage. Our results demonstrated that the essential oil does not change myocardial contractility and does not present relevant immunotoxicity.

ABSTRACT: Currently, the Brazilian savanna (Cerrado) represents the main agricultural area of the country, comprising a great variety of landscapes and soils, geological formations and vegetation patterns, as well as the major watershed. We studied the hydropedology and morphometry of a representative catchment (Frutal river), on a high tableland (Chapada) in the Triângulo Mineiro region, Brazil, describing the soil-water-landscape relationships to understand land use and water resources. To this end, we applied physical, chemical, micromorphological, and morphometric methods. When dry, compaction was observed in well-structured Ferralsols (Latossolos) with medium texture under intensive agriculture, reducing the water recharge capacity. The soil carbon stock was highest in hydromorphic savannas (veredas), reaching an organic matter content of 316.8 g kg-1 in the studied Umbric Gleysols, representing poorly drained lowlands. Physical and micromorphological properties were relevant parameters to understand the water recharge in soil; in agricultural fields, bulk density tended to increase and hydraulic conductivity to decrease, particularly under long-term sugarcane; morphometric parameters in the Frutal catchment indicated a low flooding risk and high flow capacity. This reinforces the need for soil conservation strategies to enhance water infiltration and groundwater recharge, with a view to maintain the water longer in the catchment. For surface water dynamics, slope morphology is an important property, affecting soil erosion, water retention and crop productivity.

ABSTRACT The objective of this study was to evaluate the effects of toxic levels of aluminum (Al) on the growth and external morphology of root tips in two maize genotypes with differential Al tolerance. The maize genotypes UFVM-100 (Al-sensitive) and UFVM-200 (Al-tolerant) were treated with 0 and 50 mM Al in a 0.5 mM CaCl2 solution at pH 4.5; root elongation, Al content and the external morphology of the root tips were evaluated. Chemical analysis, hematoxylin staining and energy dispersive X-ray spectrometry (EDS) showed greater Al accumulation in the root tips of the UFVM-200 genotype. The inhibition of root elongation in the UFVM-100 genotype, however, was much stronger than in the UFVM-200 genotype. Both maize genotypes exhibited visible and intense alterations in external micromorphology of the root tip, especially the Al-sensitive UFVM-100 genotype. Scanning electron micrographs showed intense cell disorganization and transverse ruptures of the protodermic and outer cortex layers of the cells in both genotypes. The ruptures were deeper and wider and reached the inner cortex layers in the UFVM-100 genotype. The EDS analysis showed that, in addition to Al accumulation, there was a proportional increase in the P concentration in the root tips of the UFVM-200 genotype. This is indicative of possible precipitation and/or immobilization of Al in the root tip apoplast of this genotype, which contributes to symplastic detoxification.

O objetivo neste estudo foi avaliar os efeitos de doses de nitrogênio associadas às doses do redutor de crescimento trinexapac-etil na cultura do trigo. O experimento, conduzido em Viçosa-MG, seguiu um esquema fatorial 5×4, no delineamento experimental de blocos casualizados, com quatro repetições. Os tratamentos foram 30, 60, 90, 120 e 150kg ha-1 de N, combinados com 0, 62,5, 125 e 187,5g ha-1 de trinexapac-etil. Trinexapac-etil promove redução da massa seca da parte aérea e do rendimento de grãos nas menores doses de N, mas sob doses elevadas de N o trinexapac-etil promove aumento dessas características. A combinação entre doses de N e trinexapac-etil que promove maior massa seca da parte aérea é de 150kg ha-1 e 187g ha-1, mas a que promove maior produção de grãos é de 100kg ha-1 e 120g ha-1, respectivamente.

The objective in this study was to evaluate the effects of nitrogen rates in association with rates of the growth retardant trinexapac-ethyl on wheat. The experiment was conducted in Viçosa, MG and arranged in a 5×4 factorial, randomized block design, with four repetitions. A combination of five nitrogen rates (30, 60, 90, 120 and 150kg ha-1) with four rates of trinexapac-ethyl (0, 62.5, 125 and 187.5g ha-1) were tested. Trinexapac-ethyl promotes reduction of soot dry mass and grain yield at the lowest N rates, but at the highest N rates there is increase in these characteristics. The combination between N and trinexapac-ethyl rates that promotes higher shoot dry mass is 150kg ha-1 and 187g ha-1 but that promotes higher grain yield is 100kg ha-1 and 120g ha-1, respectively.

Objetivou-se avaliar o efeito de doses e formas de aplicação de nitrogênio sobre o desempenho e produção de duas cultivares de trigo. O experimento, conduzido em Viçosa-MG, de maio a setembro de 2005, foi instalado segundo um esquema fatorial 2x2x5 no delineamento de blocos casualizados, com quatro repetições. Os tratamentos avaliados consistiram da combinação das cultivares de trigo, BRS 210 e Pioneiro, duas formas de aplicação de adubo nitrogenado (dose total aplicada por ocasião da semeadura ou 20 kg ha-1 na semeadura e o restante em cobertura no início da fase de perfilhamento) e cinco doses de nitrogênio (40, 60, 80, 100 e 120 kg ha-1) tendo como fonte o sulfato de amônio. A cultivar Pioneiro apresentou maior potencial produtivo que a cultivar BRS 210, mas foi menos resistente ao acamamento. O parcelamento da adubação nitrogenada proporcionou maior acamamento e não trouxe benefícios à produtividade dos grãos. As doses de N promoveram resposta quadrática da produtividade dos grãos, com ponto de máxima estimado na dose de 96,8 kg ha-1 e, aumento linear do acamamento de plantas da cultivar Pioneiro.

The objective of this work was to evaluate the effect of doses and methods of nitrogen application on the development and yield of two wheat cultivars. The experiment was conducted in Viçosa-MG, from May to September 2005, and it was arranged in a 2x2x5 factorial, randomized block design with four repetitions. The treatments consisted of the combination of the two wheat cultivars, BRS 210 and Pioneiro, two nitrogen application methods (total dose applied at seed sowing or 20 kg ha-1 at sowing and the remaining as topdressing in the beginning of tillage) and five N doses (40, 60, 80, 100 and 120 kg ha-1) with ammonium sulfate as N source. Cultivar Pioneiro showed higher yield potential than cultivar BRS 210, but it was less resistance to lodging. Splitting nitrogen fertilization increased lodging and brought no benefit to grain yield. Grain yield response to N doses was quadratic, reaching maximum at 96.8 kg ha-1, and linear increase in lodging of cultivar Pioneiro plants.

O acamamento de plantas na cultura do trigo tem sido controlado com o uso de redutores de crescimento. No entanto, há carência de informações sobre o efeito desses produtos na qualidade fisiológica de sementes. O objetivo deste estudo foi avaliar a qualidade fisiológica das sementes de trigo obtidas sob efeito de doses e épocas de aplicação de três redutores de crescimento. O experimento, conduzido em Viçosa, Minas Gerais, entre maio e setembro de 2005, foi instalado com tratamentos em esquema fatorial e hierárquico com uma testemunha, no delineamento em blocos casualizados, com quatro repetições. Os tratamentos foram 500, 1000 e 1500g ha-1 de clormequat; 40, 80 e 120g ha-1 de paclobutrazol e 62,5, 125 e 187,5g ha-1 de trinexapac-etil aplicados no estádio 6 ou 8 da escala Feeks e Large, mais uma testemunha sem aplicação de redutor. A aplicação foliar dos redutores de crescimento clormequat, paclobutrazol e trinexapac-etil não afeta a germinação e o vigor das sementes de trigo.

Plant lodging in wheat has been controlled through growth retardants. However, there is lack of information on the effect of these products on the physiological quality of seeds. The objective of this study was to evaluate the physiological quality of wheat seeds obtained under different rates and application times of three growth retardants. The trial was carried out in Viçosa-MG, from May to September 2005, in a factorial and hierarchical scheme, in randomized block design with four replications and a control treatment. The treatments consisted of 500, 1000 and 1500g ha-1 of chlormequat; 40, 80 and 120g ha-1 of paclobutrazol and 62.5, 125 and 187.5g ha-1 of trinexapac-ethyl applied at the 6 or 8 growth stage based on the scale of Feeks and Large, and a control treatment without growth retardant application. Leaf application of growth retardants as chlormequat, paclobutrazol and trinexapac-ethyl did not affect the germination and vigor of wheat seeds.

Objetivou-se com este trabalho avaliar o efeito de doses e épocas de aplicação de três reguladores de crescimento na elongação do colmo de plantas de trigo. O experimento, conduzido em Viçosa, Estado de Minas Gerais, de maio a setembro de 2005, foi instalado com tratamentos em esquema fatorial e hierárquico com uma testemunha, no delineamento em blocos casualizados com quatro repetições. Os tratamentos foram 500; 1.000 e 1.500 g ha-1 de clormequat; 62,5; 125,0 e 187,5 g ha-1 de trinexapac-etil e 40; 80 e 120 g ha-1 de paclobutrazol, aplicados no estádio 6 ou 8 da escala Feeks e Large, e uma testemunha. O comprimento do colmo com trinexapac-etil foi menor do que com o clormequat, que, por sua vez, foi menor do que com o paclobutrazol. A aplicação de reguladores na época 1 promoveu maior redução dos entrenós basais, enquanto a aplicação na época 2 proporcionou maior influência no pedúnculo. O aumento das doses de clormequat e trinexapac-etil promoveu redução do comprimento do colmo e das partes que o formam. O aumento das doses de paclobutrazol promoveu respostas pouco expressivas no colmo e seus componentes.

The objective of this work was to evaluate the effect of doses and times of application of three growth regulators on wheat stem elongation. The experiments were conducted in Viçosa, Minas Gerais State, from May to September 2005, in a factorial and hierarchical randomized block design with four repetitions and a control treatment. Treatments consisted of 500, 1000 and 1500 g ha-1 of chlormequat; 62.5, 125.0 and 187.5 g ha-1 of trinexapac-ethyl and 40, 80 and 120 g ha-1 of paclobutrazol, applied either at the 6 or 8 stage of the Feeks and Large scale, with a control. Stem length with trinexapac-ethyl was smaller than with chlormequat, which was in its turn smaller than with paclobutrazol. Application of growth regulators at stage 1 produced shorter basal internodes, whereas the stage 2 application caused greater reduction in the peduncle. The increase in chlormequat and trinexapac-ethyl doses reduced length of stem and its parts. The increase in paclobutrazol doses had very small effects on wheat growth and its parts.

The tetrazolium test is a promising alternative for rapid evaluation of the seed viability. Two described methodologies are available for coffee shrub. Thus, this study was conducted to verify what methodology is more efficient for fast determination of both viability and energy. So, seeds from four lots were subjected to procedures as follows. 1) Soaking for 24 hours at 30 ºC; cutting the portion of the endosperm containing the embryo; exposure of this portion to 0.1% tetrazolium solution for 16 hours at 35 ºC; then, the embryo is removed with the aid of a bistoury for subsequent evaluation. 2) Soaking for 24 hours at 30 ºC; removal of the embryo and its exposure to 0.1% tetrazolium solution for 16 hours at 35 ºC for subsequent evaluation. To comparing the results, the germination test accomplished and the final percent germination and the vigor (1st germination counting) were evaluated. The methodology 2 showed to be more promising for the evaluation of both viability and vigor of coffee shrub seeds, since the obtained values were equal to those of the germination test. The methodology 1 was not efficient, as showing problems concerning to coloration of the embryos, therefore generating doubts during the evaluation of the seed viability.

O teste de tetrazólio apresenta-se como uma alternativa promissora para a avaliação rápida da viabilidade das sementes, sendo que para cafeeiro há duas metodologias descritas. O presente trabalho teve como objetivo verificar, entre estas metodologias existentes, qual a mais eficiente para a determinação rápida da viabilidade e do vigor. Para tal, sementes de 4 lotes foram submetidas as seguintes metodologias: 1) embebição por 24 horas a 30 ºC; corte da porção do endosperma que contém o embrião; exposição desta porção à solução de tetrazólio a 0,1%, durante 16 horas a 35 ºC, retirando em seguida o embrião com ajuda de um bisturi para posterior avaliação; 2) embebição por 24 horas a 30 ºC; retirada do embrião e exposição à solução de tetrazólio a 0,1%, durante 16 horas a 35 ºC, para posterior avaliação. Para comparação dos resultados obtidos foi montado o teste de germinação, avaliando-se a porcentagem final de germinação e o vigor (1ª contagem de germinação). Os resultados indicaram que a metodologia 2 mostrouse mais promissora para a avaliação da viabilidade e do vigor de sementes de cafeeiro, pois os valores obtidos se equivaleram aos valores do teste de germinação. A metodologia 1 não foi eficiente pois apresentou problemas quanto à coloração dos embriões, gerando assim dúvida durante a avaliação da viabilidade das sementes.

Este estudo foi realizado com os objetivos de analisar a histologia da pele e avaliar a influência da técnica de curtimento e da posição da retirada do corpo-de-prova sobre a qualidade da pele de carpa prateada (Hypophtalmichthys molitrix). Vinte e três peixes foram insensibilizados para retirada das peles para curtimento. Amostras referentes a três peixes foram fixadas em formol 10%. Após a inclusão em parafina, foram cortadas com aproximadamente 5 mm de espessura e coradas pela técnica de hematoxilina-eosina (HE). Após o curtimento das peles, foram retirados os corpos-de-prova para os testes de resistência. Os testes foram realizados com dinamômetro EMIC, com velocidade de afastamento entre cargas de 100 ± 20 mm/mm, em ambiente climatizado a 23ºC e umidade relativa do ar de 50% por um período de 24 horas. As peles curtidas com sais de cromo apresentaram maior resistência à tração (16,96 N/mm²), ao alongamento (51,49%) e ao rasgamento progressivo (16,75 N/mm) quando comparadas àquelas curtidas sem sais de cromo (tração = 9,12 N/mm², alongamento = 17,48% e rasgamento = 11,36 N/mm). O sentido da retirada dos corpos-de-prova não interferiu nas características de tração, elongação, carga de ruptura, rasgo, força máxima e carga de força. A técnica de curtimento (utilizando ou não sais de cromo) influencia a resistência das peles de carpa prateada. A técnica empregando os sais de cromo proporciona maior resistência ao couro.

The objectives of this trial were to analyze the skin histology and to evaluate the influence of tanning technique and the direction removal of samples on skin quality of silver-plated carp (Hypophtalmichthys molitrix). Twenty-three fishes were sacrificed and the skin was removed for tanning. Samples of three fishes were settled in formol 10% and, after inclusion in paraffin, the samples were cut with approximately 5 mm of thickness and colored by the hematoxylin-eosin technique (HE). After skin tanning, the samples were removed for resistance tests. The tests were done with EMIC dynamometer, at removal speed of 100 ± 20 mm/mm, in a climatized environment (23ºC) and 50% air relative humidity for 24-h period. The skins tanned with chromium salts showed greater resistance to tensile strenght (16.96 N/mm²), elongation (51.49%) and progressive tearing (16.75 N/mm) than those tanned without chromium salts (tensile strenght = 9.12 N/mm², elongation = 17.48% and 11.36 progressive tearing = N/mm). No effect of direction removal of samples on tensile strenght, elongation, rupture load, progressive tearing, maximum force, and force load was observed. The tanning technique (with or without chromium salts) affect the resistance of the silver-plated carp skin. The technique with chromium salts results in bigger resistance to leather.