Bisphenol A (BPA), an endocrine disruptor used in different commercial polymers, is a persistent pollutant commonly found in effluents. Conventional wastewater treatment processes have low chemical removal efficiency and are expensive. This work evaluated BPA removal using laccase enzyme produced from white rot fungus Pleurotus ostreatus. For the enzymatic production, residues of acai berry and pineapple were used as laccase inducers. The use of natural inducers led to a high enzyme production (1139 and 1031 U mL−1 to acai and pineapple, respectively). Bisphenol A was removed to a concentration lower than LOD (limit of detection) after 4 h. The degradation mechanism of BPA occurred by oxidation of methyl to hydroxymethyl group in a propane portion, with breakdown of the aromatic ring. The developed technology brought to the scene a new green, viable methodology, using vegetable waste, adding value to these residues and bringing an alternative to the BPA treatment. BPA, , (BPA) polymers effluents expensive ostreatus 1139 (113 103 mL1 mL 1 mL− respectively. respectively . respectively) limit detection h portion ring green methodology waste (BPA 113 (11 10 11 (1 (

Abstract The aim was to estimate the minimum number of plants required per plot to assess the anthracnose severity by artificial inoculation of V2 stage plants. Seventy-eight carioca common bean cultivars were inoculated in 13 experiments to assess its reaction to races 65, 73, 81, and 89 of Colletotrichum lindemuthianum. Sample sizes ranged from one to nine plants were adopted to setting the number of plants per plot. A total of 1000 samplings were performed for each sample size. The following parameters were estimated in each sampling: accuracy, coefficient of variation and Pearson's correlation. The mean values of each parameter in the 1000 samplings for each sample size were subjected to quadratic regression with a plateau as a function of sample size for each experiment. Six plants per plot were needed to assess the severity of anthracnose by artificially inoculating the pathogen on common bean plants at the V2 stage. V Seventyeight Seventy eight 1 65 73 81 8 lindemuthianum 100 sampling accuracy Pearsons Pearson s correlation experiment 6 7 10

Abstract Introduction The maternal mortality rate in developing countries, such as Brazil, has significantly increased since 2020. Obstetric Emergencies (OE) account for 72.5% of these deaths. A national survey was conducted in Brazil to evaluate how gynecologists and obstetricians deal with OE and identify the main difficulties regarding theoretical/practical knowledge and structural resources. Methods An electronic questionnaire assessing resource availability, health teams, institutional protocols, and provision of OE training courses was completed by Brazilian obstetricians. Results More than 90 % of the questionnaire respondents reported treating a pregnant and/or puerperal patient with severe morbidity and that their health network has human resources, trained professionals, and structural resources required for this type of care. However, few respondents participate in continuing education programs (36 %) or specific training for the medical team (61.41 %). The implementation rates of obstetric risk identification protocols (33.09 %), a rapid response team (46.54 %), and boxes and emergency cart assembly teams (71.68 %) were determined. Conclusion A high Maternal Mortality Ratio (MMR) may be related to disorganized healthcare systems, low implementation of risk classification protocols for the care of severe maternal and fetal conditions, and lack of access to continued/specific training programs. The Brazilian MMR is multifactorial. According to obstetricians, Brazilian health services include care teams, essential medications, obstetric centers, and clinical analysis laboratories, though they lack systematized processes and permanent professional training for qualified care of OE. countries 2020 (OE 725 72 5 72.5 deaths theoreticalpractical theoretical practical availability 9 andor professionals However 36 (3 61.41 6141 61 41 (61.4 %. . 33.09 3309 33 09 (33.0 %, , 46.54 4654 46 54 (46.5 71.68 7168 71 68 (71.6 determined (MMR systems conditions continuedspecific continued multifactorial medications centers laboratories 202 7 72. 3 ( 61.4 614 6 4 (61. 33.0 330 0 (33. 46.5 465 (46. 71.6 716 (71. 20 61. (61 33. (33 46. (46 71. (71 2 (6 (4 (7

RESUMO Objetivo traduzir e validar para o português brasileiro o instrumento Childhood Hearing Loss Question Prompt List for Parents, visando apoiar a comunicação entre profissionais de saúde e famílias de crianças com perda auditiva no Brasil. Métodos abordagem metodológica compreendendo a tradução e adaptação transcultural do instrumento, seguida de validação por meio de um processo iterativo que envolveu especialistas e o público-alvo. A tradução foi realizada por tradutores bilíngues, com subsequente retrotradução e revisão por um comitê de especialistas, para assegurar a equivalência conceitual e cultural. A validação envolveu a coleta e análise de feedback de pais de crianças com perda auditiva e profissionais fonoaudiólogos, utilizando o Índice de Validade de Conteúdo para avaliar a adequação do instrumento. Resultados a análise indicou aceitação positiva do instrumento traduzido, com a maioria dos itens atingindo Índice de Validade de Conteúdo acima do limiar de 0,78, refletindo a relevância e compreensibilidade do instrumento no contexto brasileiro. Um item específico não atingiu o índice desejado, destacando a necessidade de revisão adicional para otimizar a clareza e pertinência. Conclusão o Childhood Hearing Loss Question Prompt List for Parents traduzido e validado demonstrou ser uma ferramenta eficaz para o contexto brasileiro, facilitando a comunicação centrada na família e o envolvimento em cuidados auditivos pediátricos. Este estudo reforça a importância de adaptar e validar instrumentos de avaliação para refletir as especificidades culturais e linguísticas, assegurando a aplicabilidade e eficácia no atendimento às famílias de crianças com perda auditiva. Brasil públicoalvo. públicoalvo público alvo. alvo público-alvo bilíngues cultural fonoaudiólogos 078 0 78 0,78 desejado pertinência pediátricos linguísticas 07 7 0,7 0,

ABSTRACT Purpose This study aimed to translate and validate the Childhood Hearing Loss Question Prompt List (CHLQPL) for Parents into Brazilian Portuguese, supporting communication between healthcare professionals and families of children with hearing loss in Brazil. Methods A methodological approach was employed, comprising the translation and cross-cultural adaptation of the instrument, followed by validation through an iterative process involving experts and the target audience. The translation was conducted by bilingual translators, with subsequent back-translation and review by an expert committee to ensure conceptual and cultural equivalence. Validation involved collecting and analyzing feedback from parents of children with hearing loss and audiologists, using the Content Validity Index (CVI) to assess the instrument’s appropriateness. Results The analysis indicated positive acceptance of the translated instrument, with most items achieving a CVI above the threshold of 0.78, reflecting the instrument’s relevance and comprehensibility in the Brazilian context. One specific item did not reach the desired CVI, highlighting the need for further revision to optimize clarity and relevance. Conclusion The translated and validated CHLQPL proved to be an effective tool for the Brazilian context, facilitating family-centered communication and involvement in pediatric auditory care. This study reinforces the importance of adapting and validating assessment instruments to reflect cultural and linguistic specificities, ensuring applicability and effectiveness in assistingfamilies of children with hearing loss. (CHLQPL Portuguese Brazil employed crosscultural cross instrument audience translators backtranslation back equivalence audiologists (CVI s appropriateness 078 0 78 0.78 context familycentered family centered care specificities 07 7 0.7 0.

Resumo Realizou-se este estudo com o objetivo de caracterizar a produção e a digestão anaeróbia de dejetos de suínos em terminação submetidos a restrição alimentar ou suplementados com ractopamina ou cromo. Os dejetos foram provenientes de 50 suínos machos castrados, em fase de terminação, com ± 154 dias de idade, com peso inicial de 99,0 ± 4,4 kg e final de 117,2 ± 5,8 kg. As dietas experimentais foram: controle (dieta convencional); restrição qualitativa (redução de 7,5% de energia líquida em relação à dieta controle); restrição quantitativa (redução de 15% no fornecimento de ração); cromo (0,8 mg); e ractopamina (10 ppm). Os dados foram submetidos à análise de variância por meio do delineamento em blocos ao acaso, no qual as semanas de análises foram consideradas como blocos (cofator). Não foram observadas diferenças entre as dietas nas produções de dejetos na matéria natural (MN), matéria seca (MS), matéria mineral (MM) e matéria orgânica (MO). Animais que receberam a dieta controle apresentaram o maior coeficiente de resíduo e não houve diferença entre as demais dietas. Não foram observadas diferenças entre as dietas para sólidos totais, pH e nitrogênio total dos afluentes e efluentes. O maior rendimento de biogás (574 mL g-1 de SV adicionados) foi obtido com os digestores abastecidos com dejetos de animais alimentados com dieta qualitativamente restrita. Conclui-se que a dieta com restrição qualitativa resulta em maior produção de dejetos, porém com menores excreções de nitrogênio e fósforo e maior rendimento de biogás. Realizouse Realizou se 5 castrados 15 idade 990 99 0 99, 44 4 4, 1172 117 2 117, 58 8 5, convencional convencional) redução 75 7 7,5 controle) ração ração) 0,8 08 (0, mg mg) 10 (1 ppm. ppm . ppm) acaso cofator. cofator (cofator) MN, MN , (MN) MS, MS (MS) MM (MM MO. MO (MO) totais efluentes 574 (57 g1 g 1 g- adicionados restrita Concluise Conclui 9 11 7, 0, (0 ( (cofator (MN (MS (MO 57 (5

Abstract The aim of this study was to characterize the production and anaerobic digestion of manure from finishing pigs subjected to feed restriction or supplemented with ractopamine or chromium (Cr). The waste came from 50 barrows in the finishing phase, aged ± 154 days, with a starting weight of 99.0 ± 4.4 kg and a final weight of 117.2 ± 5.8 kg. The experimental diets were as follows: control (conventional diet), qualitative restriction (7.5% reduction in net energy compared to the control diet), quantitative restriction (15% reduction in feed supply), Cr (0.8 mg), and ractopamine (10 ppm). The data were subjected to an analysis of variance using a randomized block design, in which the weeks of analysis were considered blocks (cofactors). There were no differences in manure production between the diets regarding natural matter (NM), dry matter (DM), mineral matter (MM), or organic matter (OM). Animals fed the control diet had the highest residue coefficient, and there was no difference among the other diets. No differences were observed among the diets regarding total solids, pH, or total nitrogen in the tributaries or effluents. The highest biogas yield (574 mL g-1) of added volatile solids (VS) was obtained in the digesters supplied with manure from animals fed a qualitatively restricted diet. It can be concluded that a qualitatively restricted diet results in higher manure production but with lower nitrogen and phosphorus excretion and higher biogas yields. Cr. . (Cr) 5 phase 15 days 990 99 0 99. 44 4 4. 1172 117 2 117. 58 8 5. follows conventional diet, , diet) 7.5% 75 7 (7.5 15% (15 supply, supply supply) 0.8 08 (0. mg, mg mg) 10 (1 ppm. ppm ppm) design cofactors. cofactors (cofactors) NM, NM (NM) DM, DM (DM) MM, MM (MM) OM. OM (OM) coefficient pH effluents 574 (57 g1 g 1 g-1 VS (VS yields (Cr 9 11 7.5 (7. 0. (0 ( (cofactors (NM (DM (MM (OM 57 (5 g- 7. (7

Resumo Introdução: As doenças respiratórias agudas apresentam difícil mensuração e contribuem para o grande número de causas de óbito mal definidas, o que afeta a confiabilidade das informações de mortalidade. Objetivo: Verificar a acurácia da definição da causa básica de óbito para doenças respiratórias agudas dos óbitos registrados no Sistema de Informações sobre Mortalidade do Distrito Federal em 2018. Método: Foi realizado um estudo de acurácia, com amostra mínima de 331 declarações de óbito com as causas de óbito do capítulo 10 (doenças do aparelho respiratório — J00-J99 — conforme subcategorias) da Classificação Internacional de Doenças na versão 10, considerando a definição de causa básica de óbito pela equipe de investigação como o padrão-ouro. Foram calculadas a sensibilidade, especificidade, valores preditivos positivo e negativo e as razões de verossimilhanças positivas e negativas com os respectivos intervalos de confiança a 95%. Resultados: Total de 396 declarações de óbito foram incluídas. Foi constatada alta sensibilidade para identificar doenças pulmonares agudas nas causas básicas de óbito (82,8%. IC95% 78,6–86,5) e alta especificidade (93,1%. IC95% 77,2–99,2). A probabilidade de a declaração apresentar resultado falso negativo foi baixa (0,2. IC95% 0,1–0,2). Conclusão: As declarações de óbito por doenças respiratórias agudas apresentaram bons resultados para acurácia e incitam a realização de estudos semelhantes para outras condições. Introdução definidas mortalidade Objetivo 2018 Método 33 1 J00J99 JJ J00 J99 J J00-J9 subcategorias padrãoouro. padrãoouro padrão ouro. ouro padrão-ouro 95 95% Resultados 39 incluídas 82,8%. 828 82 8 (82,8% IC95 IC 78,6–86,5 786865 78 6 86 5 93,1%. 931 93 (93,1% 77,2–99,2. 772992 77,2–99,2 . 77 2 99 77,2–99,2) 0,2. 02 0 (0,2 0,1–0,2. 0102 0,1–0,2 0,1–0,2) Conclusão condições 201 3 J00J9 J0 J9 J00-J 9 82,8% (82,8 IC9 78,6–86, 78686 7 93,1% (93,1 77299 77,2–99, 0,2 (0, 010 0,1–0, 20 J00J 82,8 (82, 78,6–86 7868 93,1 (93, 7729 77,2–99 0, (0 01 0,1–0 82, (82 78,6–8 786 93, (93 772 77,2–9 ( 0,1– (8 78,6– (9 77,2– 0,1 78,6 77,2 78, 77,

Abstract Background: Acute respiratory diseases are difficult to measure and contribute to a large number of ill-defined causes of death, which affects the reliability of mortality information. Objective: To verify the accuracy of the definition of the underlying cause of death for acute respiratory diseases registered in the Mortality Information System of the Federal District in 2018. Method: An accuracy study was conducted, with a minimum sample of 331 death certificates with the causes of death according to Chapter 10 (diseases of the respiratory system — J00-J99 — according to subcategories) of the International Classification of Diseases, version 10, considering the definition of the underlying cause of death by the research team as the gold standard. Sensitivity, specificity, positive and negative predictive values, and positive and negative likelihood ratios were calculated with their respective 95% confidence intervals (CIs). Results: Overall, 396 death certificates were included. High sensitivity was identified to determine acute lung diseases in the underlying cause of death (82.8%, 95%CI 78.6–86.5) and high specificity (93.1%, 95%CI 77.2–99.2). The probability of the declaration shows that negative false value was low (0.2, 95%CI 0.1–0.2). Conclusion: Death certificates due to acute respiratory diseases showed good results for accuracy and this study encourages similar studies for other conditions. Background illdefined ill defined information Objective 2018 Method conducted 33 1 J00J99 JJ J00 J99 J J00-J9 subcategories Diseases standard Sensitivity values 95 CIs. CIs . (CIs) Results Overall 39 included 82.8%, 828 82 8 (82.8% 95CI CI 78.6–86.5 786865 78 6 86 5 93.1%, 931 93 (93.1% 77.2–99.2. 772992 77.2–99.2 77 2 99 77.2–99.2) 0.2, 02 0 (0.2 0.1–0.2. 0102 0.1–0.2 0.1–0.2) Conclusion conditions 201 3 J00J9 J0 J9 J00-J 9 (CIs 82.8% (82.8 78.6–86. 78686 7 93.1% (93.1 77299 77.2–99. 0.2 (0. 010 0.1–0. 20 J00J 82.8 (82. 78.6–86 7868 93.1 (93. 7729 77.2–99 0. (0 01 0.1–0 82. (82 78.6–8 786 93. (93 772 77.2–9 ( 0.1– (8 78.6– (9 77.2– 0.1 78.6 77.2 78. 77.

RESUMO A banana é um fruto climatérico de vida pós-colheita curta e que sofre mudanças acentuadas durante seu amadurecimento. O armazenamento refrigerado é considerado a forma mais eficaz de preservar a qualidade dos frutos, diminuindo seu metabolismo. Assim, o trabalho teve como objetivo avaliar as características físicoquímicas de bananas 'BRS-Princesa' armazenadas sob refrigeração, visando determinar o período de vida útil pós-colheita. Os frutos foram armazenados em câmara fria, em temperatura de 14±1 ºC e 75±1% UR por períodos de 0, 7,14, 21 ou 28 dias. Após cada período, os frutos foram retirados da câmara fria e mantidos em temperatura ambiente (25±1 ºC) até apresentarem coloração da casca completamente amarela (estádio 6 de maturação). As avaliações foram realizadas logo após a retirada dos frutos da refrigeração e quando os frutos atingiram o estádio 6. A refrigeração afetou todas as variáveis de qualidade físico-química dos frutos avaliados após a retirada da câmara fria. Porém, quando os frutos atingiram o completo amadurecimento, as variáveis rendimento da polpa, sólidos solúveis e açúcares solúveis totais não apresentaram diferença significativa, indicando um amadurecimento semelhante ao controle. Frutos armazenados por 21 e 28 dias apresentaram índices de injúrias por frio. As bananas 'BRS-Princesa' podem ser armazenadas a 14 °C por um período de até 14 dias, sem prejuízo à boa aparência e qualidade dos frutos, com incremento de 8 dias na possibilidade de consumo dos frutos, quando comparados ao controle. póscolheita pós colheita metabolismo Assim BRSPrincesa BRS Princesa 'BRS-Princesa póscolheita. colheita. 141 1 14± 751 75 75±1 0 714 7 7,14 2 25±1 251 25 (25± maturação. maturação . maturação) físicoquímica físico química Porém polpa significativa controle frio C 75± 71 7,1 25± (25 7, (2 (

ABSTRACT Banana is a climacteric fruit with short post-harvest shelf-life, undergoing significant changes during ripening. Refrigerated storage is considered the most efficient method to preserve the quality of fruit by slowing down their metabolism. Thus, the objective of this work was to evaluate physical and chemical characteristics of 'BRS-Princesa' bananas stored under refrigeration, focusing on determining their post-harvest shelf-life. The fruit were stored in a cold chamber at temperature of 14±1 °C and relative air humidity of 75±1% for 0, 7, 14, 21, and 28 days. They were removed from the cold chamber after each period and kept at 25±1 °C in a climate-controlled environment (CCE) until they presented a fully yellow peel (ripening stage 6). The fruit were evaluated immediately after removal from the cold chamber and when they reached the ripening stage 6. Refrigeration affected all physical and chemical characteristics of the fruit evaluated after removal from the cold chamber. However, when the fruit were fully ripe, pulp yield, soluble solids content, and total soluble sugars presented no significant difference, indicating a similar ripening to the control group. Fruit stored for 21 and 28 days showed chilling injury. 'BRS-Princesa' bananas can be stored at 14 °C for up to 14 days, without losses in fruit appearance and quality, thus extending the time for fruit consumption by 8 days when compared to fruit stored in the CCE (25±1 °C). postharvest post harvest shelflife, shelflife shelf life, life shelf-life metabolism Thus BRSPrincesa BRS Princesa 'BRS-Princesa refrigeration shelflife. life. 141 1 14± C 751 75 75±1 0 7 2 251 25 25± climatecontrolled climate controlled (CCE 6 . 6) However ripe yield content difference group injury (25± °C. °C) 75± (25 (2 (

ABSTRACT Objective Currently programmed cell death protein 1 (PD-1) inhibitors in combination with other therapies are being evaluated to determine their efficacy in cancer treatment. However, the effect of PD-ligand (L) 1 expression on disease outcomes in stage III (EC III) non-small cell lung cancer is not completely understood. Therefore, this study aimed to assess the influence of PD-L1 expression on the outcomes of EC III non-small cell lung cancer. Methods This study was conducted on patients diagnosed with EC III non-small cell lung cancer who underwent treatment at a tertiary care hospital. PD-L1 expression was determined using immunohistochemical staining, all patients expressed PD-L1. Survival was estimated using the Kaplan-Meier method. Relationships between variables were assessed using Cox proportional regression models. Results A total of 49 patients (median age=69 years) with EC III non-small cell lung cancer and PD-L1 expression were evaluated. More than half of the patients were men, and most were regular smokers. The patients were treated with neoadjuvant chemotherapy, surgery, or sequential or combined chemotherapy and radiotherapy. The median progression-free survival of the entire cohort was 14.2 months, and the median overall survival was 20 months. There was no significant association between PD-L1 expression and disease progression, clinical characteristics, or overall survival. Conclusions PD-L1 expression was not correlated with EC III non-small cell lung cancer outcomes. Whether these findings differ from the association with immune checkpoint inhibitors remains to be addressed in future studies. PD1 PD (PD-1 However PDligand ligand L (L nonsmall non small understood Therefore PDL1 PDL L1 PD-L hospital staining PDL1. L1. KaplanMeier Kaplan Meier method models 4 age69 age 69 age=6 years men smokers surgery radiotherapy progressionfree progression free 142 14 2 14. months characteristics studies (PD- age6 6 age= (PD

Resumo As plantas tropicais exibem quase todos os tipos de respostas fenológicas conhecidas, variando de eventos quase contínuos aos explosivos breves, e de total sincronia à completa assincronia. Essa ampla variedade de padrões fenológicos está relacionada à alta biodiversidade e às diferentes interações com fatores abióticos e bióticos, como as interações planta-polinizador. Portanto, mudanças nesses fatores influenciam a fenologia das plantas e diferenças nas respostas fenológicas podem impactar o sucesso reprodutivo da espécie a aptidão das plantas. Analisou-se a variação interespecífica na ecologia reprodutiva (fenologia da floração, briologia floral, sistemas reprodutivos e polinização) de 10 espécies de Myrtaceae em floresta ciliar, na Chapada Diamantina, Brasil. Avaliaram-se os padrões e estratégias de floração, considerando a frequência e duração da fenofase a partir de observações mensais e de acordo com a escala semiquantitativa de Fournier; também analisaram-se a biologia floral, visitantes florais e conduziram-se experimentos de polinização (autopolinização autônoma, autopolinização manual, polinização cruzada manual e polinização natural/aberta) e calcularam-se os índices de autoincompatibilidade (SII) e autogamia (AI). A maioria das espécies apresentou floração anual, com duração de 1 a 5 meses, alta sincronia e estratégia do tipo pulsed-bang (i.e., floração massiva concentrada em poucos dias intercalados por um intervalo de tempo). Os padrões se repetiram entre espécies congêneres, exceto em Myrcia spp., em que metade das espécies foi classificada como “pulsed bang” e as demais apresentaram estratégia “big bang”. A estratégia de floração parece estar relacionada com o padrão de desenvolvimento das inflorescências, produção dos botões, duração e sincronia da floração dos indivíduos. Abelhas foram consideradas os polinizadores. A maioria das espécies foram auto-incompatíveis e alogâmicas. Em geral, as espécies ofertaram grandes quantidades de flores em pequenos intervalos temporais, favorecendo cruzamentos entre indivíduos com sincronia precisa. conhecidas breves assincronia bióticos plantapolinizador. plantapolinizador planta polinizador. polinizador planta-polinizador Portanto Analisouse Analisou floral ciliar Diamantina Brasil Avaliaramse Avaliaram Fournier analisaramse analisaram conduziramse conduziram autônoma natural/aberta naturalaberta natural aberta calcularamse calcularam SII (SII AI. AI . (AI) anual meses pulsedbang pulsed bang i.e., ie i (i.e. tempo. tempo tempo) congêneres spp spp. big bang. inflorescências botões polinizadores autoincompatíveis auto incompatíveis alogâmicas geral temporais precisa (AI i.e. (i.e i.e

Abstract Tropical plants exhibit virtually all known phenological responses, which can range from near-continuous to brief events, and from complete synchrony to complete asynchrony. This wide variety of phenological patterns is related to high tropical biodiversity and complex interactions with diverse abiotic and biotic factors, such as plant-pollinator interactions. Changes in these factors will therefore influence plant phenology, and differences in those phenological responses can impact reproductive success and plant fitness. We report here interspecific variations in the reproductive ecology (flowering phenology, floral biology, reproductive systems, and pollination) of 10 Myrtaceae species in a gallery forest in northeastern, Brazil. We evaluated flowering patterns and strategies, considering the frequency and duration of each phenophase from monthly observations, using the semi-quantitative Fournier scale. We also analyzed floral biology, recorded floral visitors, conducted pollination experiments (autonomous self-pollination, hand self-pollination, hand cross-pollination, and natural/open pollination), and calculated sefl-incompatibility (SII) and autogamy (AI) indices. Most species exhibited synchronous and annual flowering lasting between 1 and 5 months, with a “pulsed bang” strategy (i.e., mass flowering concentrated within just a few days, with intervals). These patterns were repeated among congeneric species, except in Myrcia, in which half of the species accompanied were classified as having a “pulsed bang” and the other half a “big bang” strategy. The flowering strategies appeared to be associated with distinct patterns of inflorescence development, bud production, flowering duration, and the timing of flowering in individual plants. Bee species were considered pollinators. Most species studied were self-incompatible and allogamous. In general, the species produced large quantities of flowers during short temporal intervals, favoring crosses between individuals having precise synchrony. nearcontinuous near continuous events asynchrony plantpollinator pollinator phenology fitness biology systems northeastern Brazil observations semiquantitative semi quantitative scale visitors autonomous selfpollination, selfpollination self pollination, self-pollination crosspollination, crosspollination cross cross-pollination naturalopen natural open , seflincompatibility sefl incompatibility SII (SII AI (AI indices months pulsed bang i.e., ie i e (i.e. days intervals. intervals . intervals) Myrcia big development production pollinators selfincompatible incompatible allogamous general i.e. (i.e i.e

RESUMO A maioria das pessoas com demência vive em países de baixa/média renda, onde recursos essenciais para a saúde cerebral, como educação de qualidade, ainda não são amplamente acessíveis. No Brasil, o analfabetismo ainda é frequente, especialmente em comunidades de baixo nível socioeconômico. O estudo PROAME teve como objetivo explorar a educação básica tardia em pessoas analfabetas como ferramenta para o aumento da reserva cognitiva. Objetivo: Investigar a relação entre nível socioeconômico com aprendizado e com desempenho em testes cognitivos, em adultos analfabetos. Métodos: Este estudo clínico de seis meses (NCT04473235) contou com 108 participantes inscritos no projeto Educação para Jovens e Adultos (EJA), dos quais 77 completaram os testes. O nível socioeconômico de cada participante foi medido usando-se: o Índice de Qualidade de Vida Urbana, o Índice de Desenvolvimento Humano Municipal e o nível socioeconômico doméstico. Avaliações cognitivas incluíram: o Teste de Recordação Seletiva Livre e Guiada (TRSLG), uma lista de palavras para avaliar leitura e a matriz Beta III. Resultados: A amostra era predominantemente feminina, com idade média de 58,5. Os participantes melhoraram a leitura (p=0,01) e o TRSLG (p=0,003). Com relação à memoria episódica, as mulheres tiveram resultados superiores aos dos homens (p=0,007) e participantes mais jovens melhoraram mais que seus colegas mais velhos (p=0,001). Não foi observada nenhuma relação entre o nível socioeconômico e o desempenho cognitivo. Conclusão: Independentemente do nível socioeconômico, participantes obtiveram resultados positivos após frequentar a educação básica. Isso sugere que a educação tardia pode ser uma medida preventiva não farmacológica importante, especialmente em países de baixa/média renda. baixamédia baixa renda cerebral qualidade acessíveis Brasil frequente cognitiva Objetivo cognitivos analfabetos Métodos NCT04473235 NCT (NCT04473235 10 EJA, EJA , (EJA) 7 usandose usando se usando-se Urbana doméstico incluíram TRSLG, (TRSLG) III Resultados feminina 585 58 5 58,5 p=0,01 p001 p 0 01 (p=0,01 p=0,003. p0003 p=0,003 . 003 (p=0,003) episódica p=0,007 p0007 007 (p=0,007 p=0,001. p0001 p=0,001 001 (p=0,001) cognitivo Conclusão importante NCT0447323 (NCT0447323 1 (EJA (TRSLG 58, p=0,0 p00 (p=0,0 p000 p=0,00 00 (p=0,003 (p=0,00 (p=0,001 NCT044732 (NCT044732 p=0, p0 (p=0, NCT04473 (NCT04473 p=0 (p=0 NCT0447 (NCT0447 p= (p= NCT044 (NCT044 (p NCT04 (NCT04 NCT0 (NCT0 (NCT

ABSTRACT The majority of people with dementia live in low or middle-income countries (LMICs) where resources that play a crucial role in brain health, such as quality education, are still not widely available. In Brazil, illiteracy remains a prevalent issue, especially in communities with lower socioeconomic status (SES). The PROAME study set out to explore basic education in illiterate adults as a means to improve cognitive reserve. Objective: This manuscript aims to explore the relationship between SES and learning, as well as cognitive outcomes, in an older illiterate population. Methods: This six-month clinical trial (NCT04473235) involved 108 participants, of which 77 concluded all assessments, enrolled in late-life basic education. SES assessments included Quality of Urban Living Index, Municipal Human Development Index and Household SES calculated for each participant. Cognitive assessments encompassed the Free and Cued Selective Reminding Test (FCSRT), a word list to assess reading, and the Beta III matrix. Results: The sample consisted primarily of women, with a mean age of 58.5. Participants improved their reading (p=0.01) and their FCSRT (p=0.003). Regarding episodic memory, women outperformed men (p=0.007) and younger participants improved more than their older counterparts (p=0.001). There was no association observed between SES and cognitive outcomes. Conclusion: Irrespective of SES, participants demonstrated positive outcomes after attending basic education. These findings highlight that late life education could be an important non-pharmacologic preventative measure, especially in LMICs. middleincome middle income LMICs (LMICs health available Brazil issue SES. . (SES) reserve Objective learning population Methods sixmonth six month NCT04473235 NCT (NCT04473235 10 7 latelife participant FCSRT, , (FCSRT) matrix Results 585 58 5 58.5 p=0.01 p001 p 0 01 (p=0.01 p=0.003. p0003 p=0.003 003 (p=0.003) memory p=0.007 p0007 007 (p=0.007 p=0.001. p0001 p=0.001 001 (p=0.001) Conclusion nonpharmacologic non pharmacologic measure (SES NCT0447323 (NCT0447323 1 (FCSRT 58. p=0.0 p00 (p=0.0 p000 p=0.00 00 (p=0.003 (p=0.00 (p=0.001 NCT044732 (NCT044732 p=0. p0 (p=0. NCT04473 (NCT04473 p=0 (p=0 NCT0447 (NCT0447 p= (p= NCT044 (NCT044 (p NCT04 (NCT04 NCT0 (NCT0 (NCT

Abstract Background: Myelomeningocele (MMC) is a neural tube defect disease. Antenatal repair of fetal MMC is an alternative to postnatal repair. Many agents can be used as tocolytics during the in utero fetal repair such as β2-agonists and oxytocin receptor antagonists, with possible maternal and fetal repercussions. This study aims to compare maternal arterial blood gas analysis between terbutaline or atosiban, as tocolytic agents, during intrauterine MMC repair. Methods: Retrospective cohort study. Patients were divided into two groups depending on the main tocolytic agent used during intrauterine MMC repair: atosiban (16) or terbutaline (9). Maternal arterial blood gas samples were analyzed on three moments: post induction (baseline, before the start of tocolysis), before extubation, and two hours after the end of the surgery. Results: Twenty-five patients were included and assessed. Before extubation, the terbutaline group showed lower arterial pH (7.347 ± 0.05 vs. 7.396 ± 0.02 for atosiban, p = 0.006) and higher arterial lactate (28.33 ± 12.76 mg.dL–1 vs. 13.06 ± 6.35 mg.dL–1, for atosiban, p = 0.001) levels. Conclusions: Patients who received terbutaline had more acidosis and higher levels of lactate, compared to those who received atosiban, during intrauterine fetal MMC repair. Background (MMC disease β2agonists βagonists β2 agonists β antagonists repercussions Methods 16 (16 9. 9 . (9) moments baseline, baseline (baseline tocolysis, tocolysis , tocolysis) extubation surgery Results Twentyfive Twenty five assessed 7.347 7347 7 347 (7.34 005 0 05 0.0 vs 7396 396 7.39 002 02 0.006 0006 006 28.33 2833 28 33 (28.3 1276 12 76 12.7 mgdL1 mgdL mg dL 1 mg.dL– 1306 13 06 13.0 635 6 35 6.3 0.001 0001 001 Conclusions (1 (9 7.34 734 34 (7.3 00 0. 739 39 7.3 0.00 000 28.3 283 2 3 (28. 127 12. mg.dL 130 13. 63 6. ( 73 (7. 7. 28. (28 (7 (2

Abstract Objective To assess the prevalence and type of chromosomal abnormalities in Brazilian couples with recurrent pregnancy loss (RPL) and compare the clinical characteristics of couples with and without chromosome abnormalities. Methods We assessed the medical records of 127 couples with a history of two or more miscarriages, referred to a tertiary academic hospital in Belo Horizonte, Brazil, from January 2014 to May 2023. Karyotype was generated from peripheral blood lymphocyte cultures, and cytogenetic analysis was performed according to standard protocols by heat-denatured Giemsa (RHG) banding. Results Abnormal karyotypes were detected in 10 couples (7.8%). The prevalence of chromosomal abnormalities was higher among females (6.3%) compared to males (2.0%), but this difference was not statistically significant (p=0.192). The mean number of miscarriages was. 3.3 ± 1.1 in couples with chromosome abnormalities and 3.1 ± 1.5 in couples without chromosome abnormalities (p=0.681). Numerical chromosomal anomalies (6 cases) were more frequent than structural anomalies. Four women presented low-grade Turner mosaicism. No differences were found between couples with and without karyotype alterations, except for maternal age, which was higher in the group with chromosome alterations. Conclusion The prevalence of parental chromosomal alterations in our study was higher than in most series described in the literature and was associated with increased maternal age. These findings suggest that karyotyping should be part of the investigation for Brazilian couples with RPL, as identifying the genetic etiology may have implications for subsequent pregnancies. RPL (RPL 12 Horizonte Brazil 201 2023 cultures heatdenatured heat denatured RHG (RHG banding 1 7.8%. 78 7.8% . 7 8 (7.8%) 6.3% 63 6 3 (6.3% 2.0%, 20 2.0% , 2 0 (2.0%) p=0.192. p0192 p p=0.192 192 (p=0.192) 33 3. 11 1. 31 15 5 p=0.681. p0681 p=0.681 681 (p=0.681) ( cases lowgrade low grade mosaicism age pregnancies 202 7.8 (7.8% 6.3 (6.3 2.0 (2.0% p019 p=0.19 19 (p=0.192 p068 p=0.68 68 (p=0.681 7. (7.8 6. (6. 2. (2.0 p01 p=0.1 (p=0.19 p06 p=0.6 (p=0.68 (7. (2. p0 p=0. (p=0.1 (p=0.6 (7 (2 p=0 (p=0. p= (p=0 (p= (p

RESUMO As aves silvestres têm grande destaque na transmissão de doenças ao homem, principalmente pela facilidade de acesso à população humana, levantando preocupações sobre o potencial impacto dessa proximidade no contexto da saúde pública. O presente estudo relata a ocorrência de ARV e PBV em aves silvestres de uma área de proteção ambiental no bioma Amazônico. Obtivemos 155 espécimes fecais de 47 espécies de aves silvestres, que foram submetidos a extração de RNA, detecção de ARV e PBV por técnicas moleculares, sequenciamento nucleotídico e análise filogenética. A prevalência de infecção por ARV foi 0,6% (1/155), sendo positivo em um indivíduo de Myrmotherula longipennis, e a prevalência de PBV foi 1,29% (2/155), afetando dois insdivíduos de Guira guira. A análise filogenética agrupou as cepas de PBV no genogrupo I, mostrando intensa relação filogenética com cepas isoladas de outras regiões geográficas. A cepa de ARV esteve mais relacionada filogeneticamente a cepas previamente circulantes na mesma região. Este é o primeiro estudo sobre detecção de ARV em aves silvestres no Brasil e o primeiro relato sobre a ocorrência de PBV em Guira guira. A circulação de ARV e PBV nesse ambiente demonstra que os ciclos desses agentes estão ocorrendo de forma natural no ecossistema silvestre, o que pode desencadear possíveis eventos de transmissão entre as espécies aviárias e humanos. Estudos adicionais são necessários para determinar a epidemiologia, origem, evolução e surgimento de novos vírus potencialmente patogênicos na Amazônia. homem humana pública Amazônico 15 4 RNA moleculares 06 0 6 0,6 1/155, 1155 1/155 , 1 (1/155) longipennis 129 29 1,29 2/155, 2155 2/155 2 (2/155) guira I geográficas região silvestre humanos epidemiologia origem Amazônia 0, 115 1/15 (1/155 12 1,2 215 2/15 (2/155 11 1/1 (1/15 1, 21 2/1 (2/15 1/ (1/1 2/ (2/1 (1/ (2/ (1 (2 (

ABSTRACT Wild birds have great prominence in the transmission of diseases to humans, mainly due to their ease of access to human populations, raising concerns about the potential impact of that proximity on public health. The present study reports ARV and PBV occurrence in wild birds from an environmental protection area in the Amazon biome, in Pará state, Brazil. We obtained 155 fecal specimens from 47 species of wild birds for RNA extraction, ARV and PBV detection utilizing molecular methods, nucleotide sequencing, and phylogenetic analysis. ARV prevalence was 0.6% (1/155), being positive in an individual of Myrmotherula longipennis, and PBV prevalence was 1.29% (2/155), affecting two individuals of Guira guira. The PBV strains were assigned to genogroup I based on phylogenetic analysis, and they shared a strong phylogenetic link with strains isolated from different geographic locations. The ARV strain was more closely related to strains that had previously circulated in the same region. The presence of ARV and PBV in this habitat suggests that infection cycles of these agents occur naturally in the wild ecosystem, potentially triggering transmission events between bird species and humans. This is the first study on ARV detection in wild birds in Brazil and the first report on the occurrence of PBV in wild Guira guira. Additional studies are required to determine the epidemiology, origin, evolution, and emergence of new potentially pathogenic viruses in the Amazon. humans populations health biome state 15 4 extraction methods sequencing analysis 06 0 6 0.6 1/155, 1155 1/155 , 1 (1/155) longipennis 129 29 1.29 2/155, 2155 2/155 2 (2/155) guira locations region ecosystem epidemiology origin evolution 0. 115 1/15 (1/155 12 1.2 215 2/15 (2/155 11 1/1 (1/15 1. 21 2/1 (2/15 1/ (1/1 2/ (2/1 (1/ (2/ (1 (2 (

RESUMO A modificação térmica da madeira é um processo que altera sua composição química, visando melhorar propriedades tecnológicas como estabilidade dimensional, redução do teor de umidade de equilíbrio, homogeneidade ou cor. Neste contexto, este estudo teve como objetivo avaliar a colorimetria e as propriedades químicas da madeira de Parkia pendula (angelim-saia) e Simarouba amara (marupá) modificada termicamente por diferentes métodos. Foram avaliados três tratamentos: T1 (pré-tratamento em estufa por uma hora a 120 °C e tratamento em estufa a 180 °C por três horas); T2 (pré-tratamento em autoclave a 125 °C por três horas sob 1,2 kgf cm-2 e tratamento em estufa a 180 °C por três horas); T3 (sem pré-tratamento, com tratamento da amostra em óleo vegetal). A madeira termicamente modificada foi avaliada em relação à madeira não tratada quanto à composição química estrutural (extrativos, lignina, holocelulose e teor de cinzas), parâmetros colorimétricos definidos através do sistema CIELAB (Commission Internationale de l´Eclairage) e variação total de cores. Detectamos um aumento significativo no teor de extrativos e uma diminuição no teor de holocelulose e lignina em T3 para ambas espécies, o que pode ser explicado pela impregnação de óleo nas amostras de madeira. A modificação térmica provocou o escurecimento superficial da madeira de ambas espécies, que foi mais pronunciado em P. pendula. Apesar da alteração colorimétrica, quimicamente não houve prejuízos para as madeiras. dimensional equilíbrio cor contexto angelimsaia angelim saia (angelim-saia marupá (marupá métodos tratamentos T prétratamento pré 12 C 18 horas) 1 2 1, cm2 cm cm- sem prétratamento, tratamento, pré-tratamento vegetal. vegetal . vegetal) extrativos, (extrativos cinzas, cinzas , cinzas) Commission l´Eclairage lEclairage l Eclairage cores espécies P colorimétrica madeiras

ABSTRACT The thermal modification of wood is a process that alters its chemical composition, aiming to improve technological properties such as dimensional stability, reduction of equilibrium moisture content, homogeneity, or color. In this context, this study aimed to evaluate the colorimetry and chemical properties of the wood of Parkia pendula (angelim-saia) and Simarouba amara (marupá) that was thermally modified by different methods. Three treatments were evaluated: T1 (pre-treatment in an oven for one hour at 120 °C and treatment in an oven at 180 °C for three hours); T2 (pre-treatment in an autoclave at 125 °C for three hours under 1.2 kgf cm-2 and treatment in an oven at 180 °C for three hours); and T3 (without pre-treatment, with sample treatment in vegetable oil). The thermally modified wood was evaluated in relation to untreated wood for structural chemical composition (extractives, lignin, holocellulose and ash content), colorimetric parameters defined through the CIELAB (Commission Internationale de l´Eclairage) system and total color variation. We detected a significant increase in extractives content and a decrease in holocellulose and lignin content in T3 for both species, which can be explained by the impregnation of oil in the wood samples. The thermal modification caused the surface darkening of the wood of both species, which was more pronounced in P. pendula. Despite the colorimetric change, there was no chemical damage to the wood. stability homogeneity context angelimsaia angelim saia (angelim-saia marupá (marupá methods T pretreatment pre 12 C 18 hours) 1 2 1. cm2 cm cm- without pretreatment, treatment, pre-treatment oil. . oil) extractives, (extractives , content) Commission l´Eclairage lEclairage l Eclairage variation species samples P change

RESUMO Objetivo: Analisar as alterações físicas e psicológicas da infodemia de COVID-19 para a população idosa do Rio de Janeiro. Método: estudo transversal, web-based survey. para conhecimento do acesso a notícias e informações sobre COVID-19 de idosos do Rio de Janeiro, durante o período de julho a dezembro de 2020. Realizou-se análise univariada e análise bivariada com aplicação de métodos estatísticos não paramétricos. Resultados: Participaram 390 idosos, predominantemente do sexo feminino (75,1%), faixa etária entre 66 e 75 anos (35,4%), casados (51,0%), raça/cor branca (60,3%), com imóvel próprio (81,8%), localizado na zona urbana (91%), ensino básico completo ou incompleto (31,8%) e aposentados ou pensionistas (79,2%). Idosos mais jovens foram significativamente afetados tanto física quanto psicologicamente pelas redes sociais quando comparado a televisão (<0,001). Conclusão: Alterações físicas e psicológicas da exposição às informações sobre a COVID-19 têm afetado a vida dos idosos, gerando impacto para esta população. Objetivo COVID19 COVID 19 COVID-1 Janeiro Método transversal webbased web based survey 2020 Realizouse Realizou se paramétricos Resultados 39 75,1%, 751 75,1% , 1 (75,1%) 6 7 35,4%, 354 35,4% 35 4 (35,4%) 51,0%, 510 51,0% 51 0 (51,0%) raçacor raça cor 60,3%, 603 60,3% 60 3 (60,3%) 81,8%, 818 81,8% 81 8 (81,8%) 91%, 91 91% (91%) 31,8% 318 31 (31,8% 79,2%. 792 79,2% . 79 2 (79,2%) <0,001. 0001 <0,001 001 (<0,001) Conclusão COVID1 COVID- 202 75,1 (75,1% 35,4 (35,4% 51,0 5 (51,0% 60,3 (60,3% 81,8 (81,8% 9 (91% 31,8 (31,8 79,2 (79,2% 000 <0,00 00 (<0,001 20 75, (75,1 35, (35,4 51, (51,0 60, (60,3 81, (81,8 (91 31, (31, 79, (79,2 <0,0 (<0,00 (75, (35, (51, (60, (81, (9 (31 (79, <0, (<0,0 (75 (35 (51 (60 (81 ( (3 (79 <0 (<0, (7 (5 (6 (8 < (<0 (<

ABSTRACT Objective: to analyze the physical and psychological changes of the COVID-19 infodemic for the older adult population of Rio de Janeiro. Method: a cross-sectional, web-based survey to find out about access to news and information about COVID-19 among older adult in Rio de Janeiro, between July and December 2020. Univariate analysis and bivariate analysis were carried out using non-parametric statistical methods. Results: 390 older adults took part, predominantly female (75.1%), aged between 66 and 75 (35.4%), married (51.0%), white (60.3%), owning their own home (81.8%), located in urban areas (91%), with complete or incomplete primary education (31.8%) and retired or pensioners (79.2%). Younger people were significantly affected both physically and psychologically by social networks when compared to television (<0.001). Conclusion: Physical and psychological changes from exposure to information about COVID-19 have affected the lives of the older adult, having an impact on this population. Objective COVID19 COVID 19 COVID-1 Janeiro Method crosssectional, crosssectional cross sectional, sectional cross-sectional webbased web based 2020 nonparametric non parametric methods Results 39 part 75.1%, 751 75.1% , 1 (75.1%) 6 7 35.4%, 354 35.4% 35 4 (35.4%) 51.0%, 510 51.0% 51 0 (51.0%) 60.3%, 603 60.3% 60 3 (60.3%) 81.8%, 818 81.8% 81 8 (81.8%) 91%, 91 91% (91%) 31.8% 318 31 (31.8% 79.2%. 792 79.2% . 79 2 (79.2%) <0.001. 0001 <0.001 001 (<0.001) Conclusion COVID1 COVID- 202 75.1 (75.1% 35.4 (35.4% 51.0 5 (51.0% 60.3 (60.3% 81.8 (81.8% 9 (91% 31.8 (31.8 79.2 (79.2% 000 <0.00 00 (<0.001 20 75. (75.1 35. (35.4 51. (51.0 60. (60.3 81. (81.8 (91 31. (31. 79. (79.2 <0.0 (<0.00 (75. (35. (51. (60. (81. (9 (31 (79. <0. (<0.0 (75 (35 (51 (60 (81 ( (3 (79 <0 (<0. (7 (5 (6 (8 < (<0 (<

RESUMEN Objetivo: Analizar las alteraciones físicas y psicológicas de la infodemia de COVID-19 en adultos mayores de Río de Janeiro. Método: Se trata de un estudio transversal, basado en la web, llevado a cabo para conocer el acceso a noticias e información sobre el COVID-19 entre adultos mayores de Río de Janeiro, realizado entre julio y diciembre de 2020. Se ejecutaron análisis univariados y bivariados utilizando métodos estadísticos no paramétricos Resultados: Participaron 390 adultos mayores, predominantemente mujeres (75,1%), con edades comprendidas entre los 66 y los 75 años (35,4%), casadas (51,0%), blancas (60,3%), vivienda propia (81,8%), situada en zona urbana (91%), estudios primarios completos o incompletos (31,8%) y jubiladas o pensionistas (79,2%). Los adultos más jóvenes fueron bastante afectados, tanto física como psicológicamente, por las redes sociales en comparación con la televisión (<0,001). Conclusiones: Los cambios físicos y psicológicos derivados de la exposición a la información sobre COVID-19 han afectado la vida de los adultos mayores de manera impactante. Objetivo COVID19 COVID 19 COVID-1 Janeiro Método transversal web 2020 Resultados 39 75,1%, 751 75,1% , 1 (75,1%) 6 7 35,4%, 354 35,4% 35 4 (35,4%) 51,0%, 510 51,0% 51 0 (51,0%) 60,3%, 603 60,3% 60 3 (60,3%) 81,8%, 818 81,8% 81 8 (81,8%) 91%, 91 91% (91%) 31,8% 318 31 (31,8% 79,2%. 792 79,2% . 79 2 (79,2%) afectados psicológicamente <0,001. 0001 <0,001 001 (<0,001) Conclusiones impactante COVID1 COVID- 202 75,1 (75,1% 35,4 (35,4% 51,0 5 (51,0% 60,3 (60,3% 81,8 (81,8% 9 (91% 31,8 (31,8 79,2 (79,2% 000 <0,00 00 (<0,001 20 75, (75,1 35, (35,4 51, (51,0 60, (60,3 81, (81,8 (91 31, (31, 79, (79,2 <0,0 (<0,00 (75, (35, (51, (60, (81, (9 (31 (79, <0, (<0,0 (75 (35 (51 (60 (81 ( (3 (79 <0 (<0, (7 (5 (6 (8 < (<0 (<