RESUMO O processo de secagem é de suma importância para a manutenção da qualidade das sementes, onde a temperatura de condução deste processo exerce influência direta sobre a germinação e vigor, sobretudo para as espécies olerícolas que são colhidas com alto teor de água. O objetivo desta pesquisa foi determinar e modelar as curvas de secagem de sementes de jiló (cultivar Tinguá-verde-claro) nas temperaturas de 35, 38, 41 e 44 ºC, bem como avaliar a qualidade fisiológica das sementes após a secagem. Foi utilizado o delineamento inteiramente casualizado, sendo quatro temperaturas de secagem (35, 38, 41 e 44 ºC) com quatro repetições. Nove modelos matemáticos foram ajustados por meio de análise de regressão não linear pelo método de Gauss-Newton, e o grau de ajuste foi avaliado com base na magnitude do coeficiente de determinação (R2), teste qui-quadrado (χ2), erro médio relativo (P) e erro médio estimado (SE). A qualidade das sementes foi avaliada pelo teste de germinação (G), condutividade elétrica (CE) e envelhecimento acelerado (EA). O modelo ‘Midilli Modificado’ é o que melhor representa as curvas de secagem das sementes de jiló nas temperaturas estudadas. Sementes com maior germinação e vigor, ou seja, com menores valores de CE e maiores taxas de germinação após EA, são alcançadas através da secagem a 35 e 38 ºC. vigor água cultivar Tinguáverdeclaro Tinguá verde claro Tinguá-verde-claro 4 ºC casualizado (35 repetições GaussNewton, GaussNewton Gauss Newton, Newton Gauss-Newton R2, R2 R , (R2) quiquadrado qui quadrado χ2, χ2 χ (χ2) P (P SE. SE . (SE) G, G (G) (CE EA. EA (EA) Midilli Modificado estudadas seja 3 (3 (R2 (χ2 (SE (G (EA ( (R (χ

ABSTRACT The drying process is paramount for maintaining seed quality, where the temperature during this process directly influences germination and vigor, especially for vegetable species harvested with high moisture content. This research aimed to determine and model the drying curves of S. aethiopicum (cultivar Tinguá-verde-claro) seeds at temperatures of 35, 38, 41, and 44 ºC, as well as to evaluate the physiological quality of the seeds after drying. A completely randomized design was used, with four drying temperatures (35, 38, 41, and 44 ºC) and four replicates. Nine mathematical models were fitted using the non-linear regression analysis by the Gauss-Newton method, and the goodness of fit was assessed based on the magnitude of the coefficient of determination (R2), chi-square test (χ2), relative mean error (P), and estimated mean error (SE). Seed quality was evaluated by germination test (G), electrical conductivity (EC), and accelerated aging (AA). The Modified Midilli model best represents the drying curves of S. aethiopicum seeds at the studied temperatures. Seeds with higher germination and vigor, meaning lower electrical conductivity values and higher germination rates after accelerated aging, are achieved through drying at 35 and 38 ºC. vigor content S cultivar Tinguáverdeclaro Tinguá verde claro Tinguá-verde-claro 41 4 ºC used (35 replicates nonlinear non linear GaussNewton Gauss Newton method R2, R2 R , (R2) chisquare chi square χ2, χ2 χ (χ2) P, P (P) SE. SE . (SE) G, G (G) EC, EC (EC) AA. AA (AA) 3 (3 (R2 (χ2 (P (SE (G (EC (AA ( (R (χ

Abstract Objective: To analyze the morbidity trend and space-time distribution clusters of confirmed COVID-19 cases in children and adolescents. Method: An ecological study of COVID-19 cases confirmed in the Information System from 2020 to 2022 in the age group from 0 to 19 years old, residents in Mato Grosso municipalities, Brazilian Midwest region. A trend analysis of the monthly morbidity rate of cases/100,000 inhabitants was used, following Prais-Winsten’s regression. A space-time distribution of the Bayesian incidence rate per 100,000 inhabitants was performed, in addition to a space-time scan to identify high-risk clusters. Results: Of all 79,592 COVID-19 cases studied, 51.6% were in females and 44.21% in people aged 15-19 years old. The mean monthly rate was 265.87 cases per 100,000 inhabitants, with a stationary trend in the period analyzed (Monthly Percentage Variation [MPV]) = 12.15; CI95%[MPV]: -0.73;26.70). The morbidity rate due to COVID-19 was higher in the female gender (283.14/ 100,000 inhabitants) and in the age group from 15 to 19 years old (485.90/100,000 inhabitants). An increasing trend was observed with a greater monthly time variation of 14.42% (CI95%[MPV]: 1.28;29.28)] among those aged from 10 to 14 years old. The primary cluster, which was also the one with the highest Relative Risk (RR = 5,16, p-value = 0.001), included 19 municipalities located in the North health macro-region. Conclusion: The findings indicated a monthly stationary trend in the study population, an increase in the age group from 10 to 14 years old, and areas at a higher risk for the disease in the North health macro-region of the state. Objective spacetime space COVID19 COVID COVID-1 adolescents Method 202 1 region cases100000 100 000 cases/100,00 used PraisWinstens Prais Winsten s regression 100000 100,00 performed highrisk high Results 79592 79 592 79,59 studied 516 51 6 51.6 4421 44 21 44.21 1519 15-1 26587 265 87 265.8 Monthly MPV [MPV] 12.15 1215 12 CI95%MPV CI95MPV CIMPV CI95% CI95 CI CI95%[MPV] 0.7326.70. 0732670 0.73 26.70 . 73 26 70 -0.73;26.70) 283.14/ 28314 283 (283.14 485.90/100,000 48590100000 485 90 (485.90/100,00 inhabitants. 1442 42 14.42 (CI95%[MPV] 1.2829.28 1282928 1.28 29.28 28 29 1.28;29.28) cluster RR 5 16 5,16 pvalue p value 0.001, 0001 0.001 , 001 0.001) macroregion. macroregion macro Conclusion population state COVID1 COVID- 20 cases10000 00 cases/100,0 10000 100,0 7959 7 59 79,5 51. 442 4 2 44.2 151 15- 2658 8 265. [MPV 12.1 121 CI9 CI95%[MPV 7326 0.7326.70 073267 073 0.7 2670 26.7 -0.73;26.70 283.14 2831 (283.1 485.90/100,00 4859010000 48 9 (485.90/100,0 144 14.4 (CI95%[MPV 2829 1.2829.2 128292 128 1.2 2928 29.2 1.28;29.28 5,1 0.00 cases1000 cases/100, 1000 100, 795 79, 44. 12. 732 0.7326.7 07326 07 0. 267 26. -0.73;26.7 283.1 (283. 485.90/100,0 485901000 (485.90/100, 14. 282 1.2829. 12829 1. 292 29. 1.28;29.2 5, 0.0 cases100 cases/100 0.7326. 0732 -0.73;26. 283. (283 485.90/100, 48590100 (485.90/100 1.2829 1282 1.28;29. cases10 cases/10 0.7326 -0.73;26 (28 485.90/100 4859010 (485.90/10 1.282 1.28;29 cases1 cases/1 0.732 -0.73;2 (2 485.90/10 485901 (485.90/1 1.28;2 cases/ -0.73; ( 485.90/1 48590 (485.90/ 1.28; -0.73 485.90/ 4859 (485.90 -0.7 485.90 (485.9 -0. 485.9 (485. -0 485. (485 - (48 (4

Resumo Antecedentes A dor do membro fantasma (DMF) ocorre após amputações e pode persistir de forma crônica e debilitante. A estimulação magnética transcraniana repetitiva (EMTr) é um método de neuromodulação não invasivo capaz de influenciar a função cerebral e modular a excitabilidade cortical. Sua eficácia no tratamento da dor crônica é promissora. Objetivo Avaliar as evidências sobre a eficácia e segurança do uso da EMTr no tratamento da DMF, observando os parâmetros de estimulação utilizados, efeitos colaterais e benefícios da terapia. Métodos Trata-se de uma revisão sistemática de artigos científicos publicados na literatura nacional e internacional utilizando plataformas eletrônicas. Resultados Foram identificados 252 artigos. Duzentas e quarenta e seis publicações foram removidas por estarem duplicadas ou atenderem aos critérios de exclusão. Após a seleção, foram revisados seis estudos, sendo dois ensaios clínicos randomizados e quatro relatos de caso. Todos os estudos avaliados indicaram algum grau de benefício da EMTr no alívio dos sintomas dolorosos, mesmo que temporariamente. A percepção da dor foi menor ao final do tratamento quando comparada ao período anterior às sessões e permaneceu durante o acompanhamento do paciente. Não houve padronização dos parâmetros de estimulação utilizados. Não houve relatos de eventos adversos graves. Os efeitos da terapia a longo prazo não foram avaliados. Conclusão Existem alguns benefícios, mesmo que temporários, no uso da EMTr para alívio dos sintomas dolorosos na DMF. A estimulação de alta frequência em M1 demonstrou um efeito analgésico significativo. Dado o potencial demonstrado, mas limitado pela escassez de estudos de alta qualidade, são necessários mais estudos controlados para estabelecer e padronizar o uso clínico do método. DMF (DMF debilitante (EMTr cortical promissora utilizados Tratase Trata se eletrônicas 25 exclusão seleção caso temporariamente paciente graves temporários M significativo demonstrado qualidade 2

Abstract Background Phantom limb pain (PLP) occurs after amputations and can persist in a chronic and debilitating way. Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive neuromodulation method capable of influencing brain function and modulating cortical excitability. Its effectiveness in treating chronic pain is promising. Objective To evaluate the evidence on the efficacy and safety of using rTMS in the treatment of PLP, observing the stimulation parameters used, side effects, and benefits of the therapy. Methods This is a systematic review of scientific articles published in national and international literature using electronic platforms. Results Two hundred and fifty two articles were identified. Two hundred and forty six publications were removed because they were duplicated or met the exclusion criteria. After selection, six studies were reviewed, those being two randomized clinical trials and four case reports. All evaluated studies indicated some degree of benefit of rTMS to relieve painful symptoms, even temporarily. Pain perception was lower at the end of treatment when compared to the period prior to the sessions and remained during patient follow-up. There was no standardization of the stimulation parameters used. There were no reports of serious adverse events. The effects of long-term therapy have not been evaluated. Conclusion There are some benefits, even if temporary, in the use of rTMS to relieve painful symptoms in PLP. High-frequency stimulation at M1 demonstrated a significant analgesic effect. Given the potential that has been demonstrated, but limited by the paucity of high-quality studies, further controlled studies are needed to establish and standardize the clinical use of the method. PLP (PLP way (rTMS noninvasive non invasive excitability promising used platforms identified criteria selection reviewed temporarily followup. followup follow up. up follow-up events longterm long term temporary Highfrequency High frequency M effect highquality high quality

RESUMO Objetivo Avaliar as coberturas vacinais e o atraso nas doses de vacinas em lactentes em seis municípios da região Sul do Brasil. Metodologia Inquérito Nacional de Cobertura Vacinal 2020, com lactentes nascidos vivos em 2017 e 2018, realizado entre setembro de 2020 e março de 2022. Foram avaliadas as coberturas de doses aplicadas, doses em dia e o tempo de atraso da aplicação. Resultados Para 4.681 lactentes analisados, as coberturas para vacinas indicadas até os 24 meses foram de 68,0% (IC95% 63,9;71,8) para doses aplicadas e 3,9% (IC95% 2,7;5,7) para doses em dia. A maioria das aplicações em atraso foi ≤ 3 meses. Para alguns reforços, 25% das aplicações atrasaram ≥ 6 meses. Conclusão Além da busca de faltosos às vacinas, são necessárias estratégias para estímulo ao cumprimento do esquema de vacinação nas idades preconizadas.

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ABSTRACT Objective To evaluate vaccination coverage and delay in vaccine dose administration in infants in six municipalities in the Southern region of Brazil. Methodology National Vaccination Coverage Survey 2020, with infants born alive in 2017 and 2018, carried out from September 2020 to March 2022. Coverage of doses administered, doses administered on time and delay in dose administration were evaluated. Results For 4681 infants analyzed, coverage for vaccines recommended up to 24 months was 68.0% (95%CI 63.9;71.8%) for doses administered and 3.9% (95%CI 2.7%;5.7%) for doses administered on time. Delay time for the majority of late vaccinations was ≤ 3 months. For some boosters, 25% of vaccine administration was delayed by ≥ 6 months. Conclusion In addition to tracking vaccine defaulters, strategies are needed to encourage compliance with the vaccination schedule at the recommended ages.

Abstract Introduction: Methylene Blue (MB) has been shown to attenuate oxidative, inflammatory, myocardial, and neurological lesions during ischemia-reperfusion and has great potential during cardiac arrest. This study aimed to determine the effects of MB combined with epinephrine during cardiac arrest on myocardial and cerebral lesions. Method: Thirty-eight male Wistar rats were randomly assigned to four groups: the sham group (SH, n = 5), and three groups subjected to cardiac arrest (n = 11 /group) and treated with EPI 20 μg.kg−1 (EPI), EPI 20 μg.kg−1 + MB 2 mg.kg−1 (EPI + MB), or saline 0.9% 0.2 ml (CTL). Ventricular fibrillation was induced by direct electrical stimulation in the right ventricle for 3 minutes, and anoxia was maintained for 5 minutes. Cardiopulmonary Resuscitation (CPR) consisted of medications, ventilation, chest compressions, and defibrillation. After returning to spontaneous circulation, animals were observed for four hours. Blood gas, troponin, oxidative stress, histology, and TUNEL staining measurements were analyzed. Groups were compared using generalized estimating equations. Results: No differences in the Returning of Spontaneous Circulation (ROSC) rate were observed among the groups (EPI: 63%, EPI + MB: 45%, CTL: 40%, p = 0.672). The mean arterial pressure immediately after ROSC was higher in the EPI+MB group than in the CTRL group (CTL: 30.5 [5.8], EPI: 63 [25.5], EPI+MB: 123 [31] mmHg, p = 0.007). Serum troponin levels were high in the CTL group (CTL: 130.1 [333.8], EPI: 3.70 [36.0], EPI +MB: 43.7 [116.31] ng/mL, p < 0.05). Conclusion: The coadministration of MB and epinephrine failed to yield enhancements in cardiac or brain lesions in a rodent model of cardiac arrest. Introduction (MB inflammatory ischemiareperfusion ischemia reperfusion Method Thirtyeight Thirty eight SH, SH (SH 5, , 5) 1 /group μgkg1 μgkg μg kg μg.kg− EPI, (EPI) mgkg1 mgkg mg mg.kg− MB, MB) 09 0 9 0.9 02 0. CTL. . (CTL) minutes CPR (CPR medications ventilation compressions defibrillation circulation hours gas stress histology analyzed equations Results (ROSC 63% 45 45% 40 40% 0.672. 0672 0.672 672 0.672) EPIMB (CTL 305 30 30. 5.8, 58 5.8 8 [5.8] 6 25.5, 255 25.5 25 [25.5] 12 31 [31 mmHg 0.007. 0007 0.007 007 0.007) 1301 130 130. 333.8, 3338 333.8 333 [333.8] 370 70 3.7 36.0, 360 36.0 36 [36.0] +MB 437 43 7 43. 116.31 11631 116 [116.31 ngmL ng mL ng/mL 0.05. 005 0.05 05 0.05) Conclusion μg.kg mg.kg 4 067 0.67 67 5. [5.8 25. [25.5 [3 000 0.00 00 13 333. 33 [333.8 37 3. 36. [36.0 116.3 1163 [116.3 0.0 06 0.6 [5. [25. [ [333. [36. 116. [116. [5 [25 [333 [36 [116 [2 [33 [11 [1

RESUMO Este artigo trata do uso de um livro didático digital acessível construído na perspectiva do desenho universal para a aprendizagem. O estudo quantitativo e qualitativo foi realizado em escolas públicas de quatro redes da Baixada Fluminense com a participação de 21 crianças com deficiências, seus professores de turmas comuns e do atendimento educacional especializado, assim como os colegas de turma. A análise, à luz da teoria histórico-cultural, problematiza os conceitos como inovação pedagógica, mediação pedagógica e uso de recursos tecnológicos na escola. aprendizagem 2 deficiências especializado turma análise históricocultural, históricocultural histórico cultural, cultural histórico-cultural escola

ABSTRACT This article focuses on the use of an accessible digital textbook built based on the perspective of the universal design for learning. The quantitative and qualitative study was carried out in public schools of four teaching networks of Baixada Fluminense, Rio de Janeiro, Brazil, with the participation of 21 students with disability, their teachers from regular classes and the specialized educational service, as well as the classmates. The analysis, according to the cultural-historical theory, problematizes concepts such as pedagogical innovation, pedagogical mediation, and use of technological resources at school. learning Fluminense Janeiro Brazil 2 disability service classmates analysis culturalhistorical cultural historical theory innovation mediation school

ABSTRACT Objective: This study aimed to compare the levels of HIF1-α, VEGF, TNF-α, and IL-10 in the peri-implant crevicular fluid of patients with and without peri-implantitis. Methods: Forty patients, comprising 16 with and 24 without peri-implantitis were selected. Results: Patients with peri-implantitis exhibited significantly higher HIF-1α levels than those without peri-implantitis (p=0.0005). TNF-α revealed significant positive correlations with IL-10 (p=0.0008) and VEGF (p=0.0246), whereas HIF-1α and IL-10 levels (p=0.0041) demonstrated a negative and significative correlation in the peri-implantitis group. Conclusion: This study, for the first time demonstrates the balance of HIF-1α, TNFα, IL-10, and VEGF in peri-implantitis. It shows an elevated HIF-1α levels in patients with peri-implantitis, which could have stemmed from persistent inflammation- triggered hypoxia. Furthermore, the positive correlation between TNF-α and VEGF suggests intensified proinflammatory activity in peri-implantitis. Nevertheless, further studies are essential to understand these immune dynamics in peri-implantitis. Objective HIF1α, HIF1α HIFα HIF1 α, α HIF HIF1-α TNFα TNF IL10 IL 10 IL-1 periimplant peri implant periimplantitis. periimplantitis implantitis. implantitis Methods 1 2 selected Results 1α p=0.0005. p00005 p p=0.0005 . 0 0005 (p=0.0005) p=0.0008 p00008 0008 (p=0.0008 p=0.0246, p00246 p=0.0246 , 0246 (p=0.0246) p=0.0041 p00041 0041 (p=0.0041 group Conclusion 1α, IL10, 10, periimplantitis, implantitis, inflammation hypoxia Furthermore Nevertheless IL1 IL- p0000 p=0.000 000 (p=0.0005 (p=0.000 p0024 p=0.024 024 (p=0.0246 p=0.004 p0004 004 (p=0.004 p000 p=0.00 00 (p=0.00 p002 p=0.02 02 (p=0.024 p00 p=0.0 (p=0.0 (p=0.02 p0 p=0. (p=0. p=0 (p=0 p= (p= (p

Resumo Objetivos: descrever a prevalência da má nutrição (baixo peso, baixa estatura e excesso de peso) em crianças de seis a 59 meses e sua distribuição espacial na cidade de Beira, Moçambique. Métodos: estudo transversal exploratório, realizado entre outubro e novembro de 2019, incluindo 407 crianças de seis a 59 meses. O cálculo da amostra foi baseado na prevalência do déficit estatura/idade. Os dados antropométricos foram analisados no Anthro e a prevalência de má nutrição apresentada por meio de mapas temáticos no Quantum Geographic Information System (QGIS). Resultados: os principais resultados mostram uma prevalência de 27,0% de baixa estatura/idade, 7,9% de baixo peso/estatura e 4,7% de excesso de peso. Conclusões: a distribuição espacial evidenciou que as áreas urbanas e periurbanas da cidade apresentavam prevalências similares das três formas de má nutrição. A prevalência da má nutrição em Beira é alta, embora o déficit estatura/idade seja a sua maior expressão, estando o excesso de peso difusamente distribuído. Objetivos 5 Moçambique Métodos exploratório 2019 40 estaturaidade idade QGIS. QGIS . (QGIS) Resultados 270 27 0 27,0 79 7 9 7,9 pesoestatura 47 4 4,7 Conclusões alta expressão distribuído 201 (QGIS 2 27, 7, 4, 20

Abstract Objectives: to describe the prevalence of malnutrition (underweight, low height, and overweight) in children aged six to 59 months and its spatial distribution in the city of Beira, Mozambique. Methods: an exploratory cross-sectional study was conducted between October and November 2019, involving 407 children aged six to 59 months. The sample size calculation was based on the prevalence of height-for-age deficit. Anthropometric data were analyzed using Anthro version and the prevalence of malnutrition was presented through thematic maps generated in Quantum Geographic Information System (QGIS). Results: the main findings revealed a prevalence of 27.0% for low height/age, 7.9% for underweight/height, and 4.7% for overweight. Conclusions: the spatial distribution highlighted that both urban and peri-urban areas of the city showed similar prevalence rates for the three forms of malnutrition. The prevalence of malnutrition in Beira is high, with deficit height/age being the most significant expression, while overweight is diffusely distributed. Objectives underweight, underweight (underweight height 5 Mozambique Methods crosssectional cross sectional 2019 40 heightforage age QGIS. QGIS . (QGIS) Results 270 27 0 27.0 heightage 79 7 9 7.9 underweightheight underweight/height 47 4 4.7 Conclusions periurban peri high expression distributed 201 (QGIS 2 27. 7. 4. 20

Resumo Objetivo: mapear a literatura científica sobre a validação de instrumentos para a avaliação da qualidade dos serviços prestados na atenção primária à saúde em doenças crônicas relacionadas com hipertensão arterial sistêmica, diabetes mellitus, hanseníase e tuberculose. Método: revisão de escopo, seguindo o método do Instituto Joanna Briggs e descrito de acordo com o Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews. Foram incluídas 13 bases de dados e literatura cinzenta. A seleção dos estudos foi realizada após a remoção de duplicatas e avaliação individual e em pares. Os dados foram extraídos com base em um roteiro elaborado e apresentados em forma de quadros e tabelas. Resultados: a análise de 28 estudos selecionados mostrou que a sua maioria era do Brasil, seguidos pela China e Malásia. Quase a metade dos instrumentos validados eram genéricos, e os específicos eram sobre avaliação em diabetes mellitus e hanseníase. Os tipos de validação realizados foram de conteúdo e de construto. Conclusão: há necessidade de construção de instrumentos específicos devido à escassez de estudos sobre o processo de validação de instrumentos para a avaliação da qualidade dos serviços prestados pela atenção primária à saúde em doenças crônicas. Objetivo sistêmica tuberculose Método escopo MetaAnalyses Meta Analyses 1 cinzenta pares tabelas Resultados 2 Brasil Malásia genéricos construto Conclusão

Abstract Objective: to map the scientific literature on the validity of instruments for evaluating the quality of services provided in primary health care for chronic diseases related to systemic arterial hypertension, diabetes mellitus, leprosy and tuberculosis. Method: scoping review, following the Joanna Briggs Institute method and described in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews. 13 databases and gray literature were included. The selection of studies was carried out after removing duplicates and individual and paired evaluation. The data was extracted based on an elaborate script and presented in tables and charts. Results: the analysis of 28 selected studies showed that the majority were from Brazil, followed by China and Malaysia. Almost half of the validated instruments were generic, and the specific ones covered the evaluation of diabetes mellitus and leprosy. The types of validation carried out were content and construct. Conclusion: there is a need to construct specific instruments due to the scarcity of studies on the process of validating instruments for evaluating the quality of services provided by primary health care for chronic diseases. Objective hypertension tuberculosis Method review MetaAnalyses Meta Analyses 1 included charts Results 2 Brazil Malaysia generic Conclusion

Resumen Objetivo: mapear la literatura científica sobre la validación de instrumentos para evaluar la calidad de los servicios prestados en la atención primaria de salud para enfermedades crónicas relacionadas con la hipertensión arterial sistémica, diabetes mellitus, lepra y tuberculosis. Método: revisión de alcance, siguiendo el método del Instituto Joanna Briggs y descrito de acuerdo con el Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews. Se incluyeron 13 bases de datos y literatura gris. La selección de los estudios se realizó previa eliminación de duplicados y evaluación individual y pareada. Los datos se extrajeron basándose en un guión elaborado y se presentaron en cuadros y tablas. Resultados: el análisis de 28 estudios seleccionados mostró que la mayoría era de Brasil, seguidos de China y Malasia. Casi la mitad de los instrumentos validados eran genéricos, y los específicos cubrían la evaluación de diabetes mellitus y lepra. Los tipos de validación realizados fueron de contenido y de constructo. Conclusión: existe la necesidad de construir instrumentos específicos debido a la escasez de estudios sobre el proceso de validación de instrumentos para evaluar la calidad de los servicios prestados por la atención primaria de salud para enfermedades crónicas. Objetivo sistémica tuberculosis Método alcance MetaAnalyses Meta Analyses 1 gris pareada tablas Resultados 2 Brasil Malasia genéricos constructo Conclusión

Abstract We predicted miRNAs with regulatory impact on NFKB1 and TRAF6 gene expression and selected the miR-194-5p, miR-124-3p, miR-9-5p, and miR-340-5p and their target genes for expression analyses on CD14+ monocytes from rheumatoid arthritis (RA) patients and healthy controls. Additionally, we evaluated the influence of genes and miRNA expression on RA patients’ cytokine levels. No difference was observed in genes or miRNAs expression when compared to healthy controls and RA patients or clinical parameters. However, we found a significant difference between miR-194-5p and miR-9-5p levels (FC=-2.31; p=0.031; FC=-3.05;p=0.031, respectively) and non-prednisone users as compared to prednisone using patients. We conducted correlation analyses to identify the strength of the relationship between expression data and cytokine plasma levels. We observed a moderate positive correlation between miR-124-3p expression and IL-6 plasma levels (r=0.46; p=0.033). In addition, overexpression of miRNAs was concomitant to TRAF6 and NFKB1 genes as indicated by correlation analyses: TRAF6 and miR-194-5p (r=0.60;p<0.001) and miR-9-5p (r=0.63;p<0.001) and NFKB1 and miR-194-5p (r=0.72;p<0.001), miR-9-5p (r=0.72;p<0.001) and miR-340-5p (r=0.61;p<0.001). NFKB1 and TRAF6 genes and miRNAs monocyte expression do not appear to be related to RA but showed a significant difference in different groups of RA therapy. In addition, increased levels of miRNAs can be linked to concomitant overexpression of TRAF6 and NFKB1 in monocytes and act as its regulators. NFKB TRAF miR1945p, miR1945p miRp miR 194 5p, 5p p miR1243p, miR1243p 124 3p, 3p miR95p, miR95p 9 miR3405p 340 CD14 CD (RA Additionally parameters However FC=2.31 FC231 FC FC= 2.31 2 31 (FC=-2.31 p=0.031 p0031 0 031 FC=3.05p=0.031, FC305p0031 FCp 3.05 p=0.031, 3 05 FC=-3.05;p=0.031 respectively nonprednisone non IL6 IL 6 IL- r=0.46 r046 r 46 (r=0.46 p=0.033. p0033 p=0.033 . 033 p=0.033) addition r=0.60p<0.001 r060p0001 rp r=0.60 p<0.001 60 001 (r=0.60;p<0.001 r=0.63p<0.001 r063p0001 r=0.63 63 (r=0.63;p<0.001 r=0.72p<0.001, r072p0001 r=0.72 , 72 r=0.72p<0.001 (r=0.72;p<0.001 r=0.61p<0.001. r061p0001 r=0.61 61 (r=0.61;p<0.001) therapy regulators 19 12 34 CD1 FC=2.3 FC23 231 2.3 (FC=-2.3 p=0.03 p003 03 05p FC=3.05p=0.031 FC305p003 305 3.0 FC=-3.05;p=0.03 r=0.4 r04 4 (r=0.4 60p r=0.60p<0.00 r060p000 r060 r=0.6 p0001 p<0.00 00 (r=0.60;p<0.00 63p r=0.63p<0.00 r063p000 r063 (r=0.63;p<0.00 72p r072p000 r072 r=0.7 7 r=0.72p<0.00 (r=0.72;p<0.00 61p r=0.61p<0.001 r061p000 r061 (r=0.61;p<0.001 1 FC=2. FC2 23 2. (FC=-2. p=0.0 p00 FC=3.05p=0.03 FC305p00 30 3. FC=-3.05;p=0.0 r=0. r0 (r=0. r=0.60p<0.0 r060p00 r06 p000 p<0.0 (r=0.60;p<0.0 r=0.63p<0.0 r063p00 (r=0.63;p<0.0 r072p00 r07 r=0.72p<0.0 (r=0.72;p<0.0 r=0.61p<0.00 r061p00 (r=0.61;p<0.00 FC=2 (FC=-2 p=0. p0 FC=3.05p=0.0 FC305p0 FC=-3.05;p=0. r=0 (r=0 r=0.60p<0. r060p0 p<0. (r=0.60;p<0. r=0.63p<0. r063p0 (r=0.63;p<0. r072p0 r=0.72p<0. (r=0.72;p<0. r=0.61p<0.0 r061p0 (r=0.61;p<0.0 (FC=- p=0 FC=3.05p=0. FC305p FC=-3.05;p=0 r= (r= r=0.60p<0 r060p p<0 (r=0.60;p<0 r=0.63p<0 r063p (r=0.63;p<0 r072p r=0.72p<0 (r=0.72;p<0 r=0.61p<0. r061p (r=0.61;p<0. (FC= p= FC=3.05p=0 FC=-3.05;p= (r r=0.60p< p< (r=0.60;p< r=0.63p< (r=0.63;p< r=0.72p< (r=0.72;p< r=0.61p<0 (r=0.61;p<0 (FC FC=3.05p= FC=-3.05;p r=0.60p (r=0.60;p r=0.63p (r=0.63;p r=0.72p (r=0.72;p r=0.61p< (r=0.61;p< FC=3.05p r=0.61p (r=0.61;p

ABSTRACT Hepatic injuries in COVID-19 are not yet fully understood and indirect pathways (without viral replication in the liver) have been associated with the activation of vascular mechanisms of liver injury in humans infected with SARS-CoV-2. Golden Syrian hamsters are an effective model for experimental reproduction of moderate and self-limiting lung disease during SARS-CoV-2 infection. As observed in humans, this experimental model reproduces lesions of bronchointerstitial pneumonia and pulmonary vascular lesions, including endotheliitis (attachment of lymphoid cells to the luminal surface of endothelium). Extrapulmonary vascular lesions are well documented in COVID-19, but such extrapulmonary vascular lesions have not yet been described in the Golden Syrian hamster model of SARS-CoV-2 infection. The study aimed to evaluate microscopic liver lesions in Golden Syrian hamsters experimentally infected with SARS-CoV-2. In total, 38 conventional Golden Syrian hamsters, divided into infected group (n=24) and mock-infected group (n=14), were euthanized at 2-, 3-, 4-, 5-, 7-, 14-, and 15-days post infection with SARS-CoV-2. Liver fragments were evaluated by histopathology and immunohistochemical detection of SARS-CoV-2 Spike S2 antigens. The frequencies of portal vein endotheliitis, lobular activity, hepatocellular degeneration, and lobular vascular changes were higher among SARS-CoV-2-infected animals. Spike S2 antigen was not detected in liver. The main results indicate that SARS-CoV-2 infection exacerbated vascular and inflammatory lesions in the liver of hamsters with pre-existing hepatitis of unknown origin. A potential application of this animal model in studies of the pathogenesis and evolution of liver lesions associated with SARS-CoV-2 infection still needs further evaluation. COVID19 COVID 19 COVID-1 without SARSCoV2. SARSCoV2 SARSCoV SARS CoV 2. 2 selflimiting self limiting SARS-CoV- attachment endothelium. endothelium . endothelium) COVID19, 19, total 3 n=24 n24 n 24 (n=24 mockinfected mock n=14, n14 n=14 , 14 (n=14) 2, 2- 3, 3- 4, 4 4- 5, 5 5- 7, 7 7- 14, 14- 15days days 15 S antigens activity degeneration SARSCoV2infected SARSCoVinfected animals preexisting pre existing origin evaluation COVID1 1 COVID- SARS-CoV n=2 n2 (n=2 n1 n=1 (n=14 n= (n= (n=1 (n

RESUMO Objetivo Analisar a associação entre o uso de dispositivo de tela no período noturno, o consumo alimentar no jantar e lanche da noite em escolares com e sem sobrepeso. Métodos Estudo transversal com uma amostra probabilística de 1.396 escolares de 7 a 14 anos de idade de escolas públicas e privadas de Florianópolis, Santa Catarina, Brasil. O consumo alimentar e a frequência de uso de dispositivos de telas do dia anterior foram obtidas por meio do questionário Consumo Alimentar e Atividades Físicas de Escolares. A associação entre o uso de dispositivo de tela no período noturno (exposição) e o consumo alimentar (desfecho) foi verificada por meio de regressão logística. Resultados No jantar, os escolares sem sobrepeso que utilizaram dispositivo de tela uma vez tiveram menor chance de consumir frutas, verduras e legumes (OR: 0,62, p=0,017) comparado com aqueles que não usaram dispositivos de telas. Além disso, aqueles que usaram dispositivo de tela duas vezes, tiveram maior chance de consumir doces (OR: 2,01, p=0,002) e a utilização de dispositivo de tela três vezes ou mais foi inversamente associado ao consumo de feijão (OR: 0,24, p=0.003), carnes, ovos e peixes (OR: 0,35, p=0,011). Os escolares com sobrepeso que utilizaram dispositivo de tela três vezes ou mais tiveram maior chance de consumirem ultraprocessados e lanches tipo pizza/hambúrguer/cachorro-quente (OR: 2,51, p=0,009). No lanche da noite, observou-se que os escolares sem sobrepeso que utilizaram dispositivo de tela três vezes ou mais, tiveram maior chance de consumir ultraprocessados e lanches (OR: 8,26; p=0,016). Conclusão Os escolares com e sem sobrepeso que utilizaram dispositivo de tela tiveram mais chances de consumir alimentos ultraprocessados. Os escolares sem sobrepeso que utilizam dispositivo de tela mais vezes a noite possuem menor chance de consumir alimentos saudáveis. 1396 1 396 1.39 Florianópolis Catarina Brasil Escolares exposição (exposição desfecho (desfecho logística frutas OR (OR 062 0 62 0,62 p=0,017 p0017 p 017 disso 201 2 01 2,01 p=0,002 p0002 002 024 24 0,24 p=0.003, p0003 p=0.003 , 003 p=0.003) carnes 035 35 0,35 p=0,011. p0011 p=0,011 . 011 p=0,011) pizza/hambúrguer/cachorroquente pizzahambúrguercachorroquente pizza/hambúrguer/cachorro quente pizza hambúrguer cachorro 251 51 2,51 p=0,009. p0009 p=0,009 009 p=0,009) observouse observou se 8,26 826 8 26 p=0,016. p0016 p=0,016 016 p=0,016) saudáveis 139 39 1.3 06 6 0,6 p=0,01 p001 20 2,0 p=0,00 p000 00 02 0,2 p=0.00 03 3 0,3 cachorroquente pizzahambúrguercachorro 25 5 2,5 8,2 82 13 1. 0, p=0,0 p00 2, p=0.0 8, p=0, p0 p=0. p=0 p=

ABSTRACT Objective Analyze the association between screen use at night, food consumption at dinner, and evening snack in schoolchildren with and without overweight. Methods Cross-sectional study with a probabilistic sample of 1396 schoolchildren from 7 to 14 years of age from public and private schools of Florianópolis, Santa Catarina, Brazil. Dietary intake and frequency of screen use of the previous day were obtained through the questionnaire Consumo Alimentar e Atividades Físicas de Escolares (Food Consumption and Physical Activities of Schoolchildren). The association between screen use at night (exposure) and consumption of food groups (outcome) according to weight status was assessed using multivariate logistic regression. Results At dinner, schoolchildren without overweight who used screens once had a lower chance of consuming fruits and vegetables (OR: 0.62, p=0.017) compared to those who did not use screens. In addition, those who used screens twice were more likely to consume sweets (OR: 2.01, p=0.002), and screen use three times or more was inversely associated with beans (OR: 0.24, p=0.003) and meat, eggs, and seafood (OR: 0.35, p=0.011) consumption. Overweight schoolchildren who used screens three times or more were more likely to consume ultra-processed foods and pizza/hamburger/hot dogs (OR: 2.51, p=0.009). For the evening snack, it was observed that schoolchildren without overweight who used screens three times or more had a greater chance of consuming ultra-processed foods and pizza/hamburger/hot dogs (OR: 8.26; p=0.016). Conclusion Overweight and non-overweight schoolchildren who used screens were more likely to consume ultra-processed foods. Schoolchildren without overweight and who use screens more often at night are less likely to consume healthy foods. dinner Crosssectional Cross sectional 139 1 Florianópolis Catarina Brazil Food Schoolchildren. . Schoolchildren) exposure (exposure outcome (outcome regression OR (OR 062 0 62 0.62 p=0.017 p0017 p 017 addition 201 2 01 2.01 p=0.002, p0002 p=0.002 , 002 p=0.002) 024 24 0.24 p=0.003 p0003 003 meat eggs 035 35 0.35 p=0.011 p0011 011 ultraprocessed ultra processed pizzahamburgerhot pizza hamburger hot 251 51 2.51 p=0.009. p0009 p=0.009 009 p=0.009) 8.26 826 8 26 p=0.016. p0016 p=0.016 016 p=0.016) nonoverweight non 13 06 6 0.6 p=0.01 p001 20 2.0 p000 p=0.00 00 02 0.2 03 3 0.3 25 5 2.5 8.2 82 0. p=0.0 p00 2. 8. p=0. p0 p=0 p=

RESUMO Fundamento: As evidências acerca das sequelas a longo prazo após internação por síndrome do desconforto respiratório agudo por COVID-19 ainda são escassas. Objetivo: Avaliar alterações nas funções pulmonar, cardíaca e renal e na qualidade de vida após internação por síndrome do desconforto respiratório agudo secundária à COVID-19. Métodos: Trata-se de estudo multicêntrico, caso-controle, incluindo 220 participantes. Os casos serão definidos como pacientes hospitalizados por síndrome do desconforto respiratório agudo devido à COVID-19. No Grupo Controle, serão selecionados indivíduos sem histórico de hospitalização nos últimos 12 meses ou sintomas a longo prazo de COVID-19. Todos os indivíduos serão submetidos à espirometria pulmonar com teste de difusão pelo monóxido de carbono, tomografia de tórax, ressonância magnética cardíaca e renal com gadolínio, ergoespirometria, creatinina sérica e urinária, proteínas totais e microalbuminúria urinária, além de questionários de qualidade de vida. Os casos serão avaliados 12 meses após a alta hospitalar e os controles, 90 dias após a inclusão no estudo. Para todas as análises estatísticas, será assumido como significativo o valor p < 0,05. Resultados: O desfecho primário do estudo foi definido com a capacidade de difusão pulmonar aferida para o monóxido de carbono a partir de 12 meses. Os demais parâmetros das funções pulmonar, cardíacas e renal e da qualidade de vida foram definidos como desfechos secundários. Conclusão: Este estudo visa determinar as sequelas a longo prazo nas funções pulmonar, cardíaca e renal e na qualidade de vida de pacientes internados por síndrome do desconforto respiratório agudo devido à COVID-19 na população brasileira. Fundamento COVID19 COVID 19 COVID-1 escassas Objetivo COVID19. 19. Métodos Tratase Trata se multicêntrico casocontrole, casocontrole caso controle, controle caso-controle 22 participantes Controle 1 tórax gadolínio ergoespirometria urinária controles 9 estatísticas 005 0 05 0,05 Resultados secundários Conclusão brasileira COVID1 COVID- 2 00 0,0 0,

ABSTRACT Rationale: Evidence about long-term sequelae after hospitalization for acute respiratory distress syndrome due to COVID-19 is still scarce. Purpose: To evaluate changes in pulmonary, cardiac, and renal function and in quality of life after hospitalization for acute respiratory distress syndrome secondary to COVID-19. Methods: This will be a multicenter case–control study of 220 participants. Eligible are patients who are hospitalized for acute respiratory distress syndrome due to COVID-19. In the control group, individuals with no history of hospitalization in the last 12 months or long-term symptoms of COVID-19 will be selected. All individuals will be subjected to pulmonary spirometry with a carbon monoxide diffusion test, chest tomography, cardiac and renal magnetic resonance imaging with gadolinium, ergospirometry, serum and urinary creatinine, total protein, and urinary microalbuminuria, in addition to quality-of-life questionnaires. Patients will be evaluated 12 months after hospital discharge, and controls will be evaluated within 90 days of inclusion in the study. For all the statistical analyses, p < 0.05 is the threshold for significance. Results: The primary outcome of the study will be the pulmonary diffusing capacity for carbon monoxide measured after 12 months. The other parameters of pulmonary, cardiac, and renal function and quality of life are secondary outcomes. Conclusion: This study aims to determine the long-term sequelae of pulmonary, cardiac, and renal function and the quality of life of patients hospitalized for acute respiratory distress syndrome due to COVID-19 in the Brazilian population. Rationale longterm long term COVID19 COVID 19 COVID-1 scarce Purpose COVID19. 19. Methods casecontrol case 22 participants group 1 selected test tomography gadolinium ergospirometry creatinine protein microalbuminuria qualityoflife questionnaires discharge 9 analyses 005 0 05 0.0 significance Results outcomes Conclusion population COVID1 COVID- 2 00 0.

RESUMO: Objetivo: Desenvolver protocolo de cuidados de enfermagem para monitorização glicêmica de pessoas com Diabetes Mellitus tipo 2, hospitalizadas. Método: Pesquisa metodológica desenvolvida em 2021 e 2022 em Manaus/AM, Brasil, em três fases: 1) Fase teórica: revisão da literatura, busca em consensos, construção de quadros analíticos, seleção dos conteúdos; 2) Fase de construção do protocolo; 3) Fase de Avaliação do protocolo realizada por enfermeiros em relação à clareza, relevância e aplicabilidade. Resultados: Revisão da literatura obteve 15 artigos e cinco consensos de sociedades científicas. O protocolo está composto por 11 itens, ressaltando a identificação de fatores de risco; manifestações de hiperglicemia e hipoglicemia; cuidados de enfermagem; processo de enfermagem e fluxograma. A avaliação dos enfermeiros foi favorável obtendo IVC de 1,0 em relação à clareza e relevância e considerado aplicável. Conclusão: O protocolo subsidiará a assistência de enfermagem na monitorização glicêmica, possibilitando melhor controle da glicemia de pessoas com diabetes hospitalizadas. RESUMO Objetivo 2 hospitalizadas Método 202 ManausAM Manaus AM Manaus/AM Brasil fases 1 teórica analíticos conteúdos 3 aplicabilidade Resultados científicas itens risco hipoglicemia fluxograma 10 0 1, aplicável Conclusão 20

ABSTRACT Objective: Developing a nursing care protocol for glycemic monitoring of hospitalized individuals with Type 2 Diabetes Mellitus. Method: Methodological research conducted in 2021 and 2022 in Manaus, state of Amazonas, Brazil, in three phases: 1) Theoretical phase: literature review, consensus search, development of analytical frameworks, content selection. 2) Protocol development phase. 3) Protocol evaluation phase conducted by nurses regarding clarity, relevance, and applicability. Results: The literature review yielded 15 articles and five consensus statements from scientific societies. The protocol consists of 11 items, with an emphasis on the identification of risk factors, manifestations of hyperglycemia and hypoglycemia, nursing care, the nursing process, and a flowchart. The nurses’ evaluation was favorable, achieving an CVI of 1.0 regarding clarity and relevance, and it was considered applicable. Conclusion: The protocol will support nursing care in glycemic monitoring, enabling better glycemic control for hospitalized individuals with diabetes. Objective Mellitus Method 202 Manaus Amazonas Brazil phases 1 search frameworks selection 3 relevance applicability Results societies items factors hypoglycemia process flowchart favorable 10 0 1. applicable Conclusion diabetes 20

RESUMEN: Objetivo: Desarrollar un protocolo de atención de enfermería para el monitoreo glucémico de personas hospitalizadas con Diabetes Mellitus tipo 2. Método: Investigación metodológica desarrollada en 2021 y 2022 en Manaos/AM, Brasil, en tres fases: 1) Fase teórica: revisión de la literatura, búsqueda de consenso, elaboración de cuadros analíticos, selección de contenidos; 2) Fase de elaboración del protocolo; 3) Fase de evaluación del protocolo realizada por enfermeros sobre la claridad, relevancia y aplicabilidad. Resultados: La revisión de la literatura obtuvo 15 artículos y cinco consensos de sociedades científicas. El protocolo consta de 11 ítems, que destacan la identificación de factores de riesgo; manifestaciones de hiperglucemia e hipoglucemia; cuidados de enfermería; proceso de enfermería y diagrama de flujo. La evaluación de los enfermeros fue favorable, se obtuvo un IVC de 1,0 para la claridad y relevancia y se consideró aplicable. Conclusión: El protocolo ayudará en los cuidados de enfermería para el monitoreo glucémico, permitiendo un mejor control de la glucemia en personas hospitalizadas con diabetes. RESUMEN Objetivo 2 Método 202 ManaosAM Manaos AM Manaos/AM Brasil fases 1 teórica consenso analíticos contenidos 3 aplicabilidad Resultados científicas ítems riesgo hipoglucemia flujo favorable 10 0 1, aplicable Conclusión diabetes 20

RESUMO Objetivos: descrever o processo de validação do aplicativo móvel TBApp para a gestão do autocuidado de pessoas com tuberculose vinculadas à Atenção Primária à Saúde. Métodos: pesquisa metodológica, desenvolvida com dez juízes especialistas, realizada por meio virtual. O aplicativo foi avaliado em relação a conteúdo e qualidade da tecnologia, em sete domínios (objetividade; estrutura e aparência; relevância; funcionalidade; confiabilidade; usabilidade; e eficiência), por meio de instrumento com escala tipo Likert. Resultados: o TBApp foi considerado válido, relevante, funcional, confiável e eficaz pelos juízes especialistas. Os domínios de objetivos, estrutura e apresentação e relevância apresentaram Índice de Validade de Conteúdo global de 0,93, e os domínios de funcionalidade, confiabilidade, usabilidade e eficiência apresentaram valores de características e subcaracterísticas maiores que 0,80. Conclusões: o TBApp é uma ferramenta criativa e inovadora, podendo ser utilizado por pessoas com TB e divulgado no meio científico. Objetivos Saúde Métodos metodológica especialistas virtual tecnologia objetividade (objetividade aparência funcionalidade confiabilidade eficiência, , eficiência) Likert Resultados válido relevante funcional objetivos 093 0 93 0,93 080 80 0,80 Conclusões inovadora científico 09 9 0,9 08 8 0,8 0,

ABSTRACT Objectives: to describe the validity process of the TBApp mobile application for self-care management for people with tuberculosis linked to Primary Health Care. Methods: methodological research developed with ten expert judges, carried out virtually. The application was assessed in relation to content and technology quality in seven domains (objectivity; structure and appearance; relevance; functionality; reliability; usability; and efficiency), using an instrument with a Likert scale. Results: TBApp was considered valid, relevant, functional, reliable and effective by expert judges. The objectives, structure and presentation and relevance domains presented an overall Content Validity Index of 0.93, and the functionality, reliability, usability and efficiency domains presented characteristics and sub-characteristics values greater than 0.80. Conclusions: TBApp is a creative and innovative tool that can be used by people with TB and disseminated in the scientific community. Objectives selfcare self care Care Methods judges virtually objectivity (objectivity appearance functionality reliability efficiency, , efficiency) scale Results valid relevant functional objectives 093 0 93 0.93 subcharacteristics sub 080 80 0.80 Conclusions community 09 9 0.9 08 8 0.8 0.

RESUMEN Objetivos: describir el proceso de validación de la aplicación móvil TBApp para la gestión del autocuidado de personas con tuberculosis, vinculada a la atención primaria de salud. Métodos: investigación metodológica desarrollada con diez jueces expertos, realizada de forma virtual. La aplicación fue evaluada en relación con la calidad del contenido y la tecnología en siete dominios (objetividad; estructura y apariencia; relevancia; funcionalidad; fiabilidad; usabilidad; y eficiencia), mediante un instrumento con escala Likert. Resultados: la TBApp fue considerada válida, relevante, funcional, confiable y eficaz por los jueces expertos. Los dominios de objetivos, estructura y presentación y relevancia presentaron un Índice de Validez de Contenido general de 0,93, y los dominios de funcionalidad, confiabilidad, usabilidad y eficiencia presentaron características y subcaracterísticas valores superiores a 0,80. Conclusiones: la TBApp es una herramienta creativa e innovadora que puede ser utilizada por personas con TB y difundida en la comunidad científica. Objetivos tuberculosis salud Métodos expertos virtual objetividad (objetividad apariencia funcionalidad fiabilidad eficiencia, , eficiencia) Likert Resultados válida relevante funcional objetivos 093 0 93 0,93 confiabilidad 080 80 0,80 Conclusiones científica 09 9 0,9 08 8 0,8 0,