This study aimed to isolate, purify and characterize secondary metabolites present in Amburana acreana leaves, native to the state of Rondônia, using classical chromatographic methods and 1D, 2D nuclear magnetic resonance spectroscopy. From the hydroethanolic leaves extract of A. acreana, p-hydroxybenzoic acid, vanillic acid, coumarin, campesterol 3-β-D-glucoside and amburoside B, all known compounds, were identified. To the best of our knowledge, this is the first report of isolation and chemical characterization of secondary metabolites in A. acreana. In addition, in vitro assays indicated that amburoside B does not have inhibitory potential on the growth of gram-negative bacteria. The presence of these compounds suggests that, thus far, amburosides (A and B) could be chemotaxonomic markers of the genus Amburana. isolate Rondônia 1D D spectroscopy A phydroxybenzoic p hydroxybenzoic acid coumarin 3βDglucoside βDglucoside 3 β glucoside identified knowledge addition gramnegative gram negative bacteria far

The in silico study aimed to suggest phytochemicals from Commiphora leptophloeos leaves essential oil as therapies against coronavirus disease (COVID-19) (or severe acute respiratory syndrome coronavirus 2, SARS-CoV-2). For that, we used molecular docking (MD), absorption, distribution, metabolism, excretion, and toxicity (ADMET) parameters, and pharmacokinetic analysis. MD showed the presence of hydrophobic and hydrogen bonds for nine compounds. β-Selinene and bicyclogermacrene showed the best interaction values with the protein Mpro (ID: 6Y2F) (-5.9) and chimeric receptor complexed with its receptor human ACE2 (ID: 6VW1) (-6.1 and -5.5) proteins, as well as low gastrointestinal and high epithelial absorptions, medium permeability for Caco-2 cells and high epithelial permeability, low oral excretion, and were inhibitors of CYP2C19 and CYP2C9 enzymes. On the other hand, both were not permeable to the blood-brain barrier and inhibitors or substrates for glycoprotein-D, and showed carcinogenic potential, but only β-selinene was considered mutagenic. Although little bioavailable, both presented aspects structurally adequate, being compounds with moderate synthesis difficulty. Therefore, more analyses are necessary to evaluate the mechanism of action and unsatisfactory parameters foreseen in bioinformatic assays. COVID19 COVID 19 (COVID-19 2 SARSCoV2. SARSCoV2 SARSCoV SARS CoV . SARS-CoV-2) that MD, , (MD) absorption distribution metabolism excretion ADMET (ADMET analysis βSelinene β Selinene ID (ID 6Y2F YF Y F 5.9 59 5 9 (-5.9 ACE 6VW1 VW 6.1 61 6 1 (-6. 5.5 55 -5.5 proteins absorptions Caco2 Caco Caco- CYPC CYP C CYP2C1 CYP2C enzymes hand bloodbrain blood brain glycoproteinD, glycoproteinD glycoprotein D, D glycoprotein-D potential βselinene selinene mutagenic bioavailable adequate difficulty Therefore assays COVID1 (COVID-1 SARS-CoV-2 (MD 5. (-5. 6VW 6. (-6 -5. (COVID- SARS-CoV- (-5 (- -5 (COVID SARS-CoV ( -

ABSTRACT Purpose: This study aimed to analyze variations in intraoperative corneal thickness during corneal cross-linking in patients with keratoconus and to investigate its possible correlation with presurgical maximal keratometry (Kmax) and pachymetry. Methods: This was a prospective case series. We used a method similar to the Dresden protocol, with the application of hydroxypropyl methylcellulose 0.1% hypo-osmolar riboflavin in corneas between 330 and 400 µm after epithelium removal. Corneal thickness was measured using portable calipers before and immediately after epithelium removal, and 30 and 60 min after the procedure. Results: The 30 patients in this study were followed up for one year. A statistically significant difference was observed in pachymetry values during the intraoperative period (p<0.0001) and an increase of 3.05 µm (95%C1: 0.56-5.54) for each diopter was seen after epithelium removal (p0.019). We found an average Kmax difference of —2.12 D between men and women (p0.013). One year after treatment, there was a statistically significant reduction in pachymetry (p<0.0001) and Kmax (p0.0170) values. Conclusions: A significant increase in pachymetry measurements was seen during the procedure, and most patients showed a regression in Kmax and pachymetry values one year after surgery. Purpose crosslinking cross linking (Kmax Methods series protocol 01 0 1 0.1 hypoosmolar hypo osmolar 33 40 3 6 procedure Results p<0.0001 p00001 p 0001 (p<0.0001 305 05 3.0 95%C1 95C1 C 95 C1 (95%C1 0.565.54 056554 0.56 5.54 56 5 54 0.56-5.54 p0.019. p0019 p0.019 . p0 019 (p0.019) 2.12 212 2 12 —2.1 p0.013. p0013 p0.013 013 (p0.013) treatment p0.0170 p00170 0170 (p0.0170 Conclusions surgery 0. 4 p<0.000 p0000 000 (p<0.000 3. 95%C 95C 9 (95%C 565 0.565.5 05655 056 0.5 554 5.5 0.56-5.5 p001 p0.01 (p0.019 2.1 21 —2. (p0.013 p0.017 p0017 017 (p0.017 p<0.00 p000 00 (p<0.00 0.565. 0565 55 5. 0.56-5. p00 p0.0 (p0.01 2. —2 p<0.0 (p<0.0 0.565 0.56-5 p0. (p0.0 — p<0. (p<0. 0.56- (p0. p<0 (p<0 (p0 p< (p< (p

Species from Pavonia genus (Malvaceae) are traditionally used as antioxidant, n anti inflammatory and antimicrobial remedies. Previous studies have revealed their diversity of specialized metabolites widely used in the development of new medicines. This is the first study on Pavonia glazioviana Gurke, a Brazilian species known as “Malva da Chapada”. The research led to identify fifteen compounds: 132 (S) hydroxy pheophytin a; and 132(S) hydroxy 173 ethoxyphaeophorbide, n decanol, cycloart 23Z ene 3β,25 diol, cycloart 24S 25 ene 3β,24 diol, sitosterol 3 O β D glucopyranoside, stigmasterol 3 O β D glucopyranoside, quercetin, acacetin, kaempferol, tiliroside, 5 hydroxy 3,7,8,4’ tetramethoxyflavone, 5,7 dihydroxy 3,8,4’ trimethoxyflavone, 5,7 dihydroxy 4’ methoxyflavone, 5,7,4’ trihydroxy 3,8 dimethoxyflavone and 5,7,4’ trihydroxy 3 methoxyflavone. The major flavonoids were tested for their antimicrobial activity, and the compound 5,7 dihydroxy 3,8,4’ trimethoxyflavone showed strong activity against Escherichia coli (MIC = 512 μg mL1), Pseudomonas aeruginosa (MIC = 512 μg mL1), Candida albicans (MIC = 512 μg mL1), Candida tropicalis (MIC = 512 μg mL1), Candida parapsilosis (MIC = 512 μg mL1), Aspergillus flavus (MIC = 512 μg mL1) and A. fumigantus (MIC = 512 μg mL1). The total phenolic content, flavonoid content, and radical scavenging potential were determined showing its great production of phenolics and flavonoids, along with interesting radical scavenging potential (DPPH EC50 = 6.36 ± 0.029 mg mL 1). Our findings contributed to characterize P. glazioviana as a source of methoxylated flavonoids with antimicrobial potential. Malvaceae (Malvaceae antioxidant remedies medicines Gurke Malva Chapada. Chapada . Chapada” compounds 13 S (S 132S 132(S 17 ethoxyphaeophorbide decanol Z 3β25 3β 3β,2 diol 2 3β24 24 glucopyranoside quercetin acacetin kaempferol tiliroside 3,7,8,4 3784 7 8 4 tetramethoxyflavone 57 5, 3,8,4 384 methoxyflavone 5,7,4 574 38 3, MIC 51 mL1, mL1 , A mL1. content DPPH EC EC5 636 6 36 6.3 0029 0 029 0.02 1. 1 1) P 3β2 3β, 3,7,8, 378 3,8, 5,7, 63 6. 002 02 0.0 3,7,8 37 00 0. 3,7, 3,7

We report the electrochemical potential of conductive filaments produced with recycled polylactic acid (rPLA) alongside a mixture of graphite (Gpt) and/or carbon black (CB) and castor oil to create an electrode ready-to-use. Additively-manufactured electrodes (AMEs) were compared with commercial-conductive filament composed of PLA and CB. The Gpt-CB-rPLA and CB-rPLA sensors showed lower charge-transfer resistance (Rct) and a greater heterogenous rate constant, k0, (Rct= 1040 ± 50 Ω and 1810 ± 30 Ω and k0= 6.91 (± 0.58) × 103 and 5.31 (± 0.40) × 103 cm s-1, respectively) compared to the sensor from the commercial filament (Rct = 9620 ± 280 Ω and k0 = 3.62 (± 0.38) ×10-3 cm s-1). The electrochemical response of [Fe(CN6)]3-/4- displayed a peak-to-peak separation of 180 ± 8 mV (Gpt-CB-rPLA) and 240 ± 6 mV (CB-rPLA) compared to commercial AME (740 ±10 mV), without any surface treatment. The filaments were used to create a 2,4,6-trinitrotoluene (TNT) detection platform with linear range of 5-20 and 5-100 µmol L-1 for Gpt-CB-rPLA and CB-rPLA, respectively. The collector-detector device was applied for the analysis of a blast simulation. Finally, it is worth noting that the results obtained are without electrode treatment/activation, indicating that the rough surface of the additively manufactured electrode is an additional feature of the device for collecting explosive residues, simply by rubbing the additively manufactured platform on different surfaces in crime scenarios. rPLA (rPLA Gpt (Gpt andor or CB (CB readytouse. readytouse ready use. use ready-to-use Additivelymanufactured Additively AMEs (AMEs commercialconductive GptCBrPLA CBrPLA chargetransfer charge transfer Rct constant k Rct= 104 5 181 3 691 91 6.9 ( 0.58 058 0 58 10 531 31 5.3 0.40 040 40 s1, s1 s 1, 1 s-1 respectively 962 28 362 62 3.6 0.38 038 38 ×103 ×10 ×10- s1. . s-1) FeCN63/4 FeCN634 FeCN Fe CN6 /4 4 CN [Fe(CN6)]3-/4 peaktopeak peak 18 (Gpt-CB-rPLA 24 (CB-rPLA 740 (74 ±1 mV, , mV) treatment 2,4,6trinitrotoluene 246trinitrotoluene trinitrotoluene 2,4,6 2 TNT (TNT 520 20 5-2 5100 100 5-10 L1 L L- CBrPLA, rPLA, collectordetector collector detector simulation Finally treatmentactivation activation treatment/activation residues scenarios 69 9 6. 0.5 05 53 5. 0.4 04 s- 96 36 3. 0.3 03 ×1 FeCN63 FeCN63/ / [Fe(CN6)]3-/ 74 (7 6trinitrotoluene 246 2,4, 52 5- 510 5-1 0. FeCN6 [Fe(CN6)]3- 7 2,4 51 [Fe(CN6)]3 2, [Fe(CN6)] [Fe(CN6) [Fe(CN6 [Fe(CN

RESUMO: A osteologia desempenha um papel indispensável para o conhecimento dos padrões de normalidade das diversas espécies, sendo a base para o entendimento zoológico. Mazama nana é bastante conhecido no Brasil, porém descrições morfológicas básicas acerca da espécie são escassas, enquanto Subulo gouazoubira, revalidação atual de Mazama gouazoubira, também é incidente no Brasil e, atualmente, tem sido alvo de diversos estudos filogenéticos. Nesse escopo, no presente estudo, foram preparadas, osteologicamente, 19 cabeças de cervídeos (16 Subulo gouazoubira e três Mazama nana) e, em seguida, tomografias computadorizadas (TCs) foram realizadas a fim de aprimorar as formas de avaliação de acidentes ósseos, além de estruturas internas e forames mediante técnicas de reconstrução em três dimensões (3D) devido a difícil visibilidade em peças intactas. Posteriormente, realizou-se um comparativo entre as reconstruções, fotografias digitais e secções tomográficas transversais do crânio, o qual possibilitou a visualização de peculiaridades e exclusividades anatômicas, como a presença da fenestra nasolacrimomaxilar, detalhamento dos seios paranasais e arquitetura de ossos finos, além do formato exclusivo dos ossos cranianos, em comparação a outras espécies, que apresentam um fenótipo único e genuíno. RESUMO espécies zoológico escassas atualmente filogenéticos escopo estudo preparadas osteologicamente 1 16 (1 seguida TCs (TCs ósseos 3D D (3D intactas Posteriormente realizouse realizou se reconstruções crânio anatômicas nasolacrimomaxilar finos cranianos genuíno (

ABSTRACT: Osteology plays an indispensable role in understanding the normal patterns of different species, serving as the foundation for zoological understanding. Mazama nana is well-known in Brazil; however, basic morphological descriptions of the species are scarce, while Subulo gouazoubira, currently revalidated as Mazama gouazoubira, is also prevalent in Brazil and has recently been the subject of various phylogenetic studies. In this respect, in the present study, 19 cervid heads (16 Subulo gouazoubira and three Mazama nana) were osteologically prepared. Next, computed tomographies (CTs) were performed to enhance the assessment of bone accidents, as well as internal structures and foramina through three-dimensional (3D) reconstruction techniques due to the difficult visibility in intact specimens. A comparison was then made between the reconstructions, digital photographs, and CT cross-sections of the skull, which enabled the visualization of anatomical peculiarities and exclusivities, such as nasolacrimal-maxillary fenestra, paranasal sinuses, and thin bone architecture, as well as the unique shape of cranial bones, compared to other species that exhibit a unique and genuine phenotype. ABSTRACT wellknown known however scarce studies respect study 1 16 (1 prepared Next CTs (CTs accidents threedimensional dimensional 3D D (3D specimens reconstructions photographs crosssections cross sections skull exclusivities nasolacrimalmaxillary nasolacrimal maxillary fenestra sinuses architecture bones phenotype (

ABSTRACT Purpose: To analyze the prevalence of lower urinary tract symptoms (LUTS) in patients who survived moderate and severe forms of COVID-19 and the risk factors for LUTS six months after hospitalization. Materials and Methods: In this prospective cohort study, patients were evaluated six months after hospitalization due to COVID-19. LUTS were assessed using the International Prostate Symptom Score. General health was assessed through the Hospital Anxiety and Depression Scale and the EQ5D-L5 scale, which evaluates mobility, ability to perform daily activities, pain and discomfort and completed a self-perception health evaluation. Results: Of 255 participants, 54.1% were men and the median age was 57.3 [44.3 – 66.6] years. Pre-existing comorbidities included diabetes (35.7%), hypertension (54.5%), obesity (30.2%) and physical inactivity (65.5%). One hundred and twenty-four patients (48.6%) had a hospital stay >15 days, 181 (71.0%) were admitted to an ICU and 124 (48.6%) needed mechanical ventilation. Median IPSS was 6 [3-11] and did not differ between genders. Moderate to severe LUTS affected 108 (42.4%) patients (40.6% men and 44.4% women; p=0.610). Nocturia (58.4%) and frequency (45.9%) were the most prevalent symptoms and urgency was the only symptom that affected men (29.0%) and women (44.4%) differently (p=0.013). LUTS impacted the quality of life of 60 (23.5%) patients with women more severely affected (p=0.004). Diabetes, hypertension, and self-perception of worse general health were associated with LUTS. Conclusions: LUTS are highly prevalent and bothersome six months after hospitalization due to COVID-19. Assessment of LUTS may help ensure appropriate diagnosis and treatment in these patients. Purpose (LUTS COVID19 COVID 19 COVID-1 Methods study COVID19. 19. Score EQ5DL5 EQDL EQ5D L5 EQ D L EQ5D-L scale mobility activities selfperception self perception evaluation Results 25 participants 541 54 1 54.1 573 57 3 57. 44.3 443 44 [44. 66.6 666 66 years Preexisting Pre existing 35.7%, 357 35.7% , 35 7 (35.7%) 54.5%, 545 54.5% 5 (54.5%) 30.2% 302 30 2 (30.2% 65.5%. 655 65.5% . 65 (65.5%) twentyfour twenty four 48.6% 486 48 (48.6% 15 >1 days 18 71.0% 710 71 0 (71.0% 12 ventilation 311 11 [3-11 genders 10 42.4% 424 42 4 (42.4% 40.6% 406 40 (40.6 444 44.4 p=0.610. p0610 p p=0.610 610 p=0.610) 58.4% 584 58 (58.4% 45.9% 459 45 9 (45.9% 29.0% 290 29 (29.0% (44.4% p=0.013. p0013 p=0.013 013 (p=0.013) 23.5% 235 23 (23.5% p=0.004. p0004 p=0.004 004 (p=0.004) Diabetes Conclusions COVID1 COVID- DL EQ5DL EQD 54. 44. [44 66. 35.7 (35.7% 54.5 (54.5% 30.2 (30.2 65.5 (65.5% 48.6 (48.6 > 71.0 (71.0 31 [3-1 42.4 (42.4 40.6 (40. p061 p=0.61 61 58.4 (58.4 45.9 (45.9 29.0 (29.0 (44.4 p001 p=0.01 01 (p=0.013 23.5 (23.5 p000 p=0.00 00 (p=0.004 [4 35. (35.7 (54.5 30. (30. 65. (65.5 48. (48. 71. (71. [3- 42. (42. 40. (40 p06 p=0.6 58. (58. 45. (45. 29. (29. (44. p00 p=0.0 (p=0.01 23. (23. (p=0.00 [ (35. (54. (30 (65. (48 (71 [3 (42 (4 p0 p=0. (58 (45 (29 (44 (p=0.0 (23 (35 (54 (3 (65 (7 ( p=0 (5 (2 (p=0. (6 p= (p=0 (p= (p

Résumé Le but de la recherche est de présenter la mise en oeuvre d’une approche pédagogique envisageant une expérimentation didactique sur les spectres atomiques en articulation avec différents genres textuels, visant à interpréter les aspects d’un événement sur la côte brésilienne, qui a causé um grand impact environnemental. L’approche a été développée em trois étapes exées sur l’expérimentation didactique, dont la construction théorique est nommée Cycle d’Expérimentation Didactique (Cycle), composé de cinq phases. Après la mise em place des trois étapes, il y a eu um moment de réflexion sur les apports et les possibilites du Cycle. L’activité a été meise em oeuvre dans une université publique brésilienne, avec de futurs professeurs de sciences dans l’enseignement de base. Les fondements méthodologiques de l’enquête sont de nature qualitative, centrés sur les processus des activités et sur les interactions verbales entre les participants. Nous avons constate que la proposition donnait lieu à une expérimentation didactique au-delà du maniement du dispositif expérimental et de l’enchaînement de prodédure déterminées hors contexte, em le mettant em relation avec le champ théorique du thème et em articulation avec les enjeux des contextes expérientiels et culturels, ainsi que situe par rapport aux inférences des participants. Il s’agit donc d’une approche fructueuse aux fins de l’enseignement des sciences aux futurs enseignants, dans une perspective d’éducation de base.

Resumen El objetivo de la investigación es presentar la implementación de um enfoque educativo que contempla la experimentación didáctica sobre espectros atómicos em articulación com diferentes géneros textuales, com el objetivo de interpretar aspectos de um acontecimento em la costa brasileña, que causó um gran impacto ambiental. El enfoque se desarrolló em tres etapas enfocadas a la experimentación didáctica, cuya construcción teórica se denomina Ciclo de Experimentación Didáctica (Ciclo), compuesto por cinco fases. Luego de la implementación de las três etapas, hubo um momento de reflexión sobre los aportes y possibilidades del Ciclo. La actividad fue implementada em uma universidad pública brasileña, con futuros professores de ciências em la educación básica. Los fundamentos metodológicos de la investigación son de carácter cualitativo, centrados em los processos de las actividades y en las interaciones verbales entre los participantes. Encontramos que la propuesta dio lugar a la experimentación didáctica más allá del manejo del aparato experimental y la secuencia de determinados procedimentos fuera del contexto, colocándolo em relación com el campo teórico del tema y en articulación com cuestiones de los contextos vivenciales y culturales, así como situados em relación con las inferencias de los participantes. Por lo tanto, es um enfoque fructífero para los propósitos de la educación em ciencias para los futuros maestros, com miras a la educación básica.

Resumo O propósito da pesquisa é apresentar a implementação de uma abordagem educacional contemplando a experimentação didática sobre os espectros atômicos em articulação com distintos gêneros textuais, visando interpretar aspectos de uma ocorrência no litoral brasileira, causadora de grande impacto ambiental. A abordagem foi desenvolvida em três etapas focadas na experimentação didática, cuja construção teórica é nomeada de Ciclo de Experimentação Didática (Ciclo), composto por cinco fases. Após a implementação das três etapas, ocorreu um momento de reflexão sobre as contribuições e possibilidades do Ciclo. A atividade foi implementada em uma universidade pública brasileira, junto a futuros professores das ciências na educação básica. Os fundamentos metodológicos da investigação são de cunho qualitativo, focados nos processos das atividades e nas interações verbais entre os participantes. Constatamos que a proposta ensejou a experimentação didática além do manuseio do aparato experimental e da sequência de procedimentos determinados alheios ao contexto, situando-a em relação ao campo teórico da temática e em articulação com questões dos contextos vivencial e cultural, bem com situada em relação as inferências dos participantes. Logo, trata-se de uma abordagem profícua para os propósitos da educação científica de futuros professores, com vistas a educação básica.

Abstract The purpose of the sesearch is to presente the implementation of an educational approach contemplating didactic experimentation on atomic spectra in articulation with different textual genres, aiming to interpret aspects of an occurrence on the brazilian coast, which caused a great environmental impact. The approach was developed in three stages focused on didactic experimentation, whose theoretical construction is named Cycle of Didactic Experimentation (Cycle), composed of five phases. After the implementation of the three stages, there was a moment of relfection on the contributions and possibilities of the Cycle. The activity was implemented in a brazilian public university, with future science teachers in basic education. The methodological foundations of the investigation are of a qualitative nature, focused on the processes of the activities and on the verbal interactions between the participants. We found that the proposal gave rise to didactic experimentation beyond the handling of the experimental apparatus and the sequence of determined procedures outside the context, placing it in relation to the theoretical field of the theme and in articulation with issues of the experiential and cultural contexts, as well as situated in relation to the inferences of the participants. Therefore, it is a fruitful approach for the purposes of science education for future teachers, with a view to basic education.

Resumo Objetivo Avaliar o desempenho de cirurgiões na ressecção de deformidades tipo CAME fazendo uso de um simulador realista de cirurgia artroscópica. Métodos Um simulador artroscópico foi criado a partir de materiais de baixo custo com auxílio de uma impressora 3D GTMax Core A1 e dos programas Invesalius e Meshmixer 2017, que foram utilizados para criar peças de cabeça de fêmur em material ABS, com a presença de uma deformidade tipo CAME, a fim de mimetizar uma situação de impacto femoroacetabular. Após as operações serem realizadas por 16 cirurgiões, os fêmures foram comparados a um modelo prévio com e outro sem deformidade pelo programa Cloudcompare, sendo avaliados ao final parâmetros como diferença volumétrica entre os fêmures operados, com e sem deformidade, distância mínima e máxima entre eles, porcentagem da deformidade ressecada, tempo estimado para ressecção total da deformidade, além de uma análise qualitativa feita com base nas imagens e gráficos fornecidos pelo programa representando as áreas das peças efetivamente ressecadas. Resultados A velocidade média de resseção foi de 34,66mm3/minuto (DP = 46 mm3/min, max = 147,33 mm3/min; min = −2,66 mm3/min). A média de ressecção obtida foi de 26,2% (DP = 34,7%, max = 111%; min = −2%). A análise qualitativa demonstrou uma hiporresecção das deformidades e, por vezes, hiperresecção de áreas não deformadas. O simulador foi muito bem avaliado pelos cirurgiões, tendo uma sensação tátil bem semelhante à cirurgia real segundo os mesmos. Conclusão Simuladores artroscópicos se mostraram muito úteis no treinamento de cirurgiões menos experientes. artroscópica D 2017 ABS femoroacetabular 1 Cloudcompare operados eles ressecada ressecadas 3466mm3minuto mmminuto 34 66mm3 minuto mm DP 4 mm3min mmmin mm3 mm3/min 14733 147 33 147,3 266 2 66 −2,6 mm3/min. . mm3/min) 262 26 26,2 347 7 34,7% 111% 111 −2%. −2% −2%) vezes deformadas mesmos experientes 201 3 66mm 1473 14 147, 6 −2, 26, 34,7 11 −2 20 34, −

Abstract Objective To evaluate surgeons’ performance in resecting CAM-type deformities using a realistic arthroscopic surgery simulator. Methods An arthroscopic simulator was created using low-cost materials with the help of a GTMax Core A1 3D printer and the programs Invesalius and Meshmixer 2017, which were used to develop femoral head parts in ABS material, with the presence of a CAM-type deformity, to mimic a femoroacetabular impact situation. After the operations were performed by 16 surgeons, the femurs were compared to a previous model with deformity and another without, using Cloudcompare, and parameters such as the volumetric difference between the operated femurs, with and without deformity, the minimum and maximum distance between them, the percentage of the deformity resected, the estimated time for total resection of the deformity, as well as a qualitative analysis based on the images and graphs provided by the program representing the areas of the parts resected, were evaluated at the end. Results The average resection speed was 34.66 mm3/min (SD = 46 mm3/min, max = 147.33; min = −2.66). The average resection rate was 26.2% (SD = 34.7%, max = 111; min = −2). Qualitative analysis showed hyporesection of deformities and sometimes hyperresection of nondeformed areas. The simulator was highly rated by the surgeons, with a tactile sensation very similar to real surgery, according to them. Conclusion Arthroscopic simulators have proved very useful in training less experienced surgeons. surgeons CAMtype CAM type lowcost low cost A D 2017 material situation 1 Cloudcompare them resected end 3466 34 66 34.6 mm3min mmmin mm3 mm SD 4 147.33 14733 147 33 −2.66. 266 −2.66 . 2 −2.66) 262 26 26.2 347 7 34.7% 111 −2. −2 −2) 201 346 3 6 34. 147.3 1473 14 −2.6 26. 34.7 11 − 20 147.

Abstract Introduction. Vitamin D is required for bone and mineral metabolism and participates in the regulation of the immune response. It is also linked to several chronic diseases and conditions, usually in populations of European descent. Brazil presents a high prevalence of vitamin D deficiency and insufficiency despite the widespread availability of sunlight in the country. Thus, it is important to investigate the role of vitamin D as a risk factor for disease and to establish causal relationships between vitamin D levels and health-related outcomes in the Brazilian population. Objective. To examine genetic variants identified as determinants of serum vitamin D in genome-wide association studies of European populations and check whether the same associations are present in Brazil. If so, these single nucleotide polymorphisms (SNPs) could be developed locally as proxies to use in genetically informed causal inference methods, such as Mendelian randomization. Materials and methods. We extracted SNPs associated with vitamin D from the genome-wide association studies catalog. We did a literature search to select papers ascertaining these variants and vitamin D concentrations in Brazil. Results. GC was the gene with the strongest association with vitamin D levels, in agreement with existing findings in European populations. However, VDR was the most investigated gene, regardless of its non-existing association with vitamin D in the genomewide association studies. Conclusions. More research is needed to validate sound proxies for vitamin D levels in Brazil, for example, prioritizing GC rather than VDR.

Resemen Introducción. La vitamina D es necesaria para el metabolismo óseo y mineral, y participa en la regulación de la respuesta inmunitaria. También está relacionada con enfermedades crónicas en poblaciones europeas. En Brasil, existe una prevalencia elevada de deficiencia e insuficiencia de vitamina D, a pesar de la amplia disponibilidad de luz solar. Por lo tanto, es importante investigar el papel de la vitamina D como factor de riesgo de diversas enfermedades y establecer relaciones causales entre los niveles de vitamina D y los problemas de salud en la población brasileña. Objetivo. Examinar variantes genéticas relacionadas con la vitamina D sérica en estudios de asociación genómica de poblaciones europeas y comprobar si estas mismas están presentes en Brasil. De ser así, estos SNPs podrían utilizarse como proxies en métodos de inferencia causal, tales como la aleatorización mendeliana. Materiales y métodos. A partir del catálogo de estudios de asociación de genoma completo se extrajeron SNPs relacionados con los niveles de vitamina D. Luego se hizo una búsqueda bibliográfica para identificar los artículos que evaluaran estos SNPs y la concentración de vitamina D en Brasil. Resultados. GC fue el gen más fuertemente asociado con los niveles de vitamina D, en concordancia con los resultados existentes en poblaciones europeas. Sin embargo, el gen VDR fue el más investigado, aunque no esté vinculado con la vitamina D en los estudios de asociación de genoma completo. Conclusiones. Se necesita más investigación para validar proxies genéticos de los niveles de vitamina D en Brasil y se recomienda priorizar el gen GC en lugar de VDR.

ABSTRACT Tamarind (Tamarindus indica L.) is a species with promising potential for commercial production; therefore, studies involving practices of conducting culture in the initial stage are essential. The present study aimed to evaluate different shading levels and substrate compositions in the seedling production of tamarind. The experimental design was completely randomized in a 4×4 factorial scheme, four levels of shading (0%, 18%, 30%, and 50%) and four substrates (pine bark-based substrate pure and mixed with 50% vermiculite, and sphagnum peat-based substrate pure and mixed with 50% vermiculite), with four replicates of four seedlings. Growth and biometric relationships were evaluated at 40 and 98 d after transplantation (DAT). The results reported that seedlings formed on the sphagnum peat-based substrate on pure or associated with vermiculite at 98 DAT had a height greater than 35 cm and a total 8.5 g DM. In contrast, seedlings produced in pine bark-based substrate did not reach 20 cm in height and had less than 3 g total DM. The condition of 30% and 50% shading promoted greater growth in height, reaching an average height greater than 40 cm, while in the full sun environment, seedlings showed an average of less than 35 cm; however, for the other variables, full sun environment did not differ from 30% shading, producing good quality seedlings. Thus, the cultivation of seedlings in an environment with 0% or 30% shading, associated with the sphagnum peat-based substrate pure or mixed with 50% vermiculite promoted the production of tamarind seedlings with vigorous growth. In the best environments, the best substrates increased, on average, 41.3% number of leaves, 80.5% shoot dry mass, 56.8% root dry mass, and 71.0% total dry mass. In the best substrates, the best environments increased, on average, 18.0% number of leaves, 20.7% shoot dry mass, 8.7% root dry mass, and 14.3% total dry mass.

RESUMO Esta pesquisa teve como objetivo calibrar e testar o modelo de cultura SARRA-H (Système d’Analyse Régionale des Risques Agroclimatologiques) para o caupi, bem como realizar o zoneamento de risco climático para esta cultura em uma região localizada na Amazônia Oriental, a fim de permitir a identificação de locais e datas de semeadura que favoreçam a produção considerando tanto o déficit hídrico como a probabilidade de ocorrência de chuvas severas durante o período da colheita. O modelo foi calibrado e validado com dados de experimentos realizados entre 2013 e 2016 no município de Castanhal, PA. As áreas de baixo risco climático foram definidas como aquelas que apresentaram índice de satisfação da necessidade hídrica (WRSI) maior ou igual a 0,5 na fase reprodutiva combinado com a ocorrência de chuvas abaixo de 20 mm na colheita por, pelo menos, 80% dos anos onde foi simulado o plantio. O modelo foi capaz de simular o balanço hídrico, crescimento e desenvolvimento do feijão-caupi nas condições climáticas e de solo da região estudada com boa precisão e exatidão. O período de ótima semeadura para o feijão-caupi compreende o intervalo entre 5 e 25 de junho para regiões localizadas acima de 2° S, e entre 25 de março e 15 de abril para regiões abaixo de 2º S. SARRAH SARRA H Système dAnalyse d Analyse Agroclimatologiques caupi Oriental 201 Castanhal PA WRSI (WRSI 05 0 0, 2 por menos 80 plantio feijãocaupi feijão exatidão S 1 º 8

ABSTRACT This study aimed to calibrate and test the SARRA-H (Système d’Analyse Régionale des Risques Agroclimatologiques) crop model for cowpea, as well as conducting a climate risk zoning for this crop in a region located in Eastern Amazon, allowing the identification of locations and sowing dates that favor the production considering both the water deficit and the probability of occurrence of severe rains during the harvest period. The model was calibrated and validated with data from experiments conducted between 2013 and 2016 in the municipality of Castanhal, PA, Brazil. Low climate risk areas were defined as those that had a water requirement satisfaction index (WRSI) greater than or equal to 0.5 in the reproductive phase combined with the occurrence of rainfall below 20 mm at the harvest for, at least, 80% of the years for which planting was simulated. The model was able to simulate the water balance, growth and development of cowpea under the climate and soil conditions of the studied location with high precision and accuracy. The optimal period for sowing cowpea comprises the interval between June 5th and 25th for regions located above 2° S and between March 25th and April 15th for regions below 2º S. SARRAH SARRA H Système dAnalyse d Analyse Agroclimatologiques Amazon 201 Castanhal PA Brazil WRSI (WRSI 05 0 5 0. 2 least 80 simulated balance accuracy th º 8

RESUMO As antocianinas presentes no açaí podem manchar o cimento de ionomero de vidro modificado por resina (CIVMR) devido a baixa resistencia ao manchamento do material. Objetivo O objetivo desse estudo foi avaliar se o açaí compromete a cor e a rugosidade de superficie de um CIVMR in vitro. Materials e Método Amostras (2 mm de espessura, 8 mm de diámetro) de Vitremer™ (3M ESPE, St Paul, MN, USA) foram preparadas de acordo com as instrugoes do fabricante. O materialfoi espatulado, inserido em um molde de silicone colocado entre duas tiras de poliestireno e fotopolimerizado. Após, as amostras foram randomizadas e alocadas em tres grupos (n=25) de acordo com as solugoes usadas para a degradagao química: saliva artificial (controle) e sorbet de açaí e suco de açaí. Utilizou-se o espectrofotometro CM-2600d/2500d (Konica Minolta, Tokyo, Japan) para a análise da cor (escala CIELa*b*) e o rugosímetro Surfcorder SE 1700 (Kosaka Corp, Tokyo, Japan) para a rugosidade de superficie (Ra, mm). As amostras foram submetidas a tres imersoes diárias (6 ml, 15 minutos) em cada solugao por 14 dias a 37°C, tendo sido lavadas em água destilada e mantidas em saliva fresca (30 minutos) nos intervalos. Após a terceira imersao no dia, as amostras foram mantidas em saliva renovada até o dia seguinte. As variáveis foram analisadas antes (L*, a*, b*, Ra) e depois da degradagao química (L’*, a’*, b’*, Ra’). Resultados Os valores de pH da saliva, sorbet e suco foram, respectivamente 7,0, 3,8 e 4,9. Houve diferenga significante para ΔE* entre saliva (p=0.005) e suco (p=0.002) e entre suco e sorbet (p=0.019), mas nao entre saliva e sorbet (p=0.401). Nao foi observada diferenga significante para Δb* entre as solugoes. Nao houve diferenga significante para Δa* entre suco e sorbet, mas eles foram significativamente diferentes da saliva (p<0.001). A luminosidade (L*) mostrou alteragao significante. O suco mostrou os maiores valores de ΔE* (7,8) e ΔL* (7,7)”. Nao houve mudanga significante para a rugosidade e nao foi observada diferenga significante entre as solugoes para ARa (p>0.05). Conclusao O açaí e a saliva causaram manchamento inaceitável do glaze do CIVMR e insignificante alteragao da rugosidade.

ABSTRACT Coloured compounds (anthocyanins) in açaí can stain resin-modified glass-ionomer cement (RMGIC) due to its low staining resistance. Aim The aim of this study was to assess whether açaí compromises the surface colour and roughness of RMGIC in vitro. Materials and Method Disc-shaped specimens (2 mm thick, 8 mm in diameter) of Vitremer™ (3M ESPE, St Paul, MN, USA) were prepared according to the manufacturer ’s instructions. The mixture was inserted into a silicone mouldplaced between two mylar strips, and light cured. Specimens were randomly divided into three groups (n=25) according to the solutions to be used for chemical degradation: artificial saliva (control), açaí sorbet and açaí juice. A spectrophotometer CM-2600d/2500d (Konica Minolta, Tokyo, Japan) was used to analyse the colour (CIELa*b* scale). Surface roughness (Ra, mm) was measuredusing theprofilometer Surfcorder SE 1700 (Kosaka Corp, Tokyo, Japan). The specimens were subjected to three daily soaks (6 ml, 15 minutes) for 14 days at 37°C. They were washed in distilled water and placed in fresh saliva (30 minutes in the interval). After the third soak in a day, they were stored in fresh saliva overnight. Outcomes were analysed at baseline (L*, a*, b*, Ra) and after degradation (L’*, a’*, b’*, Ra’). Results The pH values of saliva, sorbet, and juice were 7.0, 3.8, and 4.9, respectively. ΔE* values were 6.6 for saliva, 6.9 for sorbet and 7.8 for juice. There was a significant ΔE* difference between saliva (p=0.005) and juice (p=0.002), and between juice and sorbet (p=0.019), but none between saliva and sorbet (p=0.401). There was no significant Δb* difference between the solutions. No difference between juice and sorbet was observed for Δa*, but they were significantly different from saliva (p<0.001). Brightness (L*) changed significantly. Juice showed the highest ΔE* (7.8) and ΔL* (7.7). No significant change was observed for roughness and there was no difference between the solutions for ARa. Conclusions Açaí and saliva led to unacceptable staining, but no significant roughness changes in the resin-modified glass-ionomer cement.

RESUMO Objetivo: Avaliar o conhecimento das mães acerca da exposição solar relacionada com níveis séricos de vitamina D no período neonatal. Métodos: Estudo observacional, analítico e transversal, realizado de agosto de 2020 a maio de 2021 por meio de questionário dirigido às mães de recém-nascidos, em uma maternidade no sul do Brasil. Resultados: De 141 entrevistadas, 132 (93,6%) acreditam ser importante expor o lactente ao sol no primeiro mês de vida, 101 (71,6%) acham que essa exposição aumenta os níveis de vitamina D, 86 (61,0%) receberam tal informação de um médico, 108 (76,6%) acreditam que expor o neonato ao sol não causa riscos para a saúde, 88 (62,4%) acham que não é necessário usar proteção contra radiação solar ao expor o neonato ao sol, e 96 (68,1%) afirmaram que apenas a exposição ao sol basta para manter os níveis adequados de vitamina D durante o período neonatal. Apenas duas mães (1,4%) afirmaram que não se deve expor o neonato ao sol e uma (0,7%) que a exposição solar pode causar problemas de pele. Conclusões: A maioria das mães não possui conhecimento satisfatório acerca da exposição solar relacionada aos níveis séricos de vitamina D no período neonatal. É notável a necessidade de informar e esclarecer a população sobre a exposição solar nesse período, além de disseminar a maneira adequada de manter os níveis séricos de vitamina D. Objetivo neonatal Métodos observacional transversal 202 recémnascidos, recémnascidos recém nascidos, nascidos recém-nascidos Brasil Resultados 14 entrevistadas 13 93,6% 936 93 6 (93,6% vida 10 71,6% 716 71 (71,6% 8 61,0% 610 61 0 (61,0% médico 76,6% 766 76 (76,6% saúde 62,4% 624 62 4 (62,4% 9 68,1% 681 68 1 (68,1% 1,4% (1,4% 0,7% 07 7 (0,7% pele Conclusões 20 93,6 (93,6 71,6 (71,6 61,0 (61,0 76,6 (76,6 62,4 (62,4 68,1 (68,1 1,4 (1,4 0,7 (0,7 2 93, (93, 71, (71, 61, (61, 76, (76, 62, (62, 68, (68, 1, (1, 0, (0, (93 (71 (61 (76 (62 (68 (1 (0 (9 (7 (6 (

ABSTRACT Objective: To assess mothers’ knowledge on sun exposure related to serum vitamin D levels in the neonatal period. Methods: Observational, analytical and cross-sectional study, carried out from August 2020 to May 2021 through a questionnaire directed to mothers of newborns, in a maternity hospital in Southern Brazil. Results: From 141 interviewees, 132 (93.6%) believe it is important to expose the neonate to sun, 101 (71.6%) think this exposure can increase vitamin D levels, 86 (61%) received such information from a doctor, 108 (76.6%) believe there are no risks of sun exposure, 88 (62.4%) claim it isn´t necessary to use any kind of protection, 96 (68.1%) said that only exposure to the sun was necessary to maintain adequate levels of vitamin D during the neonatal period. Only two mothers (1.4%) claim that you should not exposure the neonate to the sun, and only one (0.7%) stated that sun expose can cause skin problems. Conclusions: Most mothers lack satisfactory knowledge about sun exposure related to serum vitamin D levels in the neonatal period. The need to inform and clarify the population about sun exposure during this period is remarkable, in addition to disseminating the proper way to maintain serum levels of vitamin D. Objective Methods Observational crosssectional cross sectional study 202 newborns Brazil Results 14 interviewees 13 93.6% 936 93 6 (93.6% 10 71.6% 716 71 (71.6% 8 61% 61 (61% doctor 76.6% 766 76 (76.6% 62.4% 624 62 4 (62.4% isnt isn t protection 9 68.1% 681 68 1 (68.1% 1.4% (1.4% 0.7% 07 0 7 (0.7% problems Conclusions remarkable 20 93.6 (93.6 71.6 (71.6 (61 76.6 (76.6 62.4 (62.4 68.1 (68.1 1.4 (1.4 0.7 (0.7 2 93. (93. 71. (71. (6 76. (76. 62. (62. 68. (68. 1. (1. 0. (0. (93 (71 ( (76 (62 (68 (1 (0 (9 (7

Résumé Contrairement aux domaines de la santé, de l’éducation et de l’assistance sociale, les Conseils Locaux de Politique Urbaine restent peu explorés dans le champ académique. Ces instances participatives jouent un rôle central dans la promotion du droit à la ville, s’opposant au modèle historique d’urbanisation inégal au Brésil. Dans ce contexte, l’expérience d’institutionnalisation de ces conseils au Brésil et leur capacité de décision restent à être étudiées. L’objectif de cet article est d’évaluer l’impact des variables de conception institutionnelle et du cycle politique sur leur capacité de décision, à partir d’une approche longitudinale entre 2009 et 2019 dans les métropoles brésiliennes. La méthode de régression logistique binomiale a été utilisée pour analyser les enregistrements de discussions, de délibérations et de représentations dans 195 réunions, impliquant 7 759 participants. Les résultats révèlent des défis persistants dans les espaces d’institutionnalisation de la démocratie délibérative par rapport au contexte local de mise en œuvre de la politique urbaine, indiquant des orientations renouvelées pour la recherche. santé léducation l éducation lassistance assistance sociale académique ville sopposant s opposant durbanisation d urbanisation lexpérience expérience dinstitutionnalisation institutionnalisation étudiées Lobjectif L objectif dévaluer évaluer limpact impact dune une 200 201 brésiliennes discussions 19 réunions 75 participants urbaine recherche 20 1 2

Resumen A diferencia de las áreas de la salud, educación y la asistencia social, los Consejos Locales de Política Urbana aún son objetos de estudio poco explorados. Tales instancias participativas tienen un papel central en la promoción del derecho a la ciudad, en contraposición al patrón histórico de urbanización desigual brasilero. En este contexto, la experiencia de institucionalización de tales consejos en Brasil y su capacidad de toma de decisión aún deben ser investigadas. El objetivo del presente artículo es evaluar el impacto de las variables de diseño institucional y del ciclo político en la capacidad de toma de decisión, a partir de un abordaje longitudinal entre los años 2009 y 2019 en las metrópolis brasileñas. El método de regresión logística binomial es utilizado para analizar los registros de discusión, deliberación y representación en 195 reuniones, involucrando 7.759 participantes. Los resultados revelan desafíos persistentes en los espacios de institucionalización de la democracia deliberativa en relación con el escenario local de implementación de la política urbana, orientándolo para agendas renovadas de investigación. salud social explorados ciudad brasilero contexto investigadas 200 201 brasileñas discusión 19 reuniones 7759 7 759 7.75 participantes urbana investigación 20 1 775 75 7.7 2 77 7.

Resumo Diferentemente das áreas da saúde, educação e assistência social, os Conselhos Locais de Política Urbana permanecem como objetos pouco explorados pelo campo. Tais instâncias participativas possuem papel central na promoção do direito à cidade, contrapondo-se ao padrão histórico de urbanização desigual brasileiro. Neste contexto, a experiência de institucionalização de tais conselhos no Brasil e sua capacidade decisória permanecem por serem investigadas. O objetivo do presente artigo é avaliar o impacto de variáveis de desenho institucional e de ciclo político em sua capacidade decisória, a partir de uma abordagem longitudinal entre os anos de 2009 e 2019 em metrópoles brasileiras. O método de regressão logística binomial foi utilizado para analisar os registros de discussão, deliberação e representação em 195 reuniões, envolvendo 7.759 participantes. Os resultados desvelam desafios persistentes nos espaços de institucionalização de democracia deliberativa em relação ao cenário local de implementação da política urbana apontando para agendas renovadas de pesquisa. saúde social campo cidade contrapondose contrapondo se brasileiro contexto investigadas 200 201 brasileiras discussão 19 reuniões 7759 7 759 7.75 participantes pesquisa 20 1 775 75 7.7 2 77 7.

Abstract Unlike the health, education, and social assistance policies, Local Councils related to Urban Policy remain little explored by the field. These participatory bodies play a central role in promoting the right to the city, countering Brazil’s historically unequal pattern of urbanization. In this scenario, these councils’ institutionalization and decision-making capacity still need to be investigated. This article aims to evaluate the impact of institutional design and political cycle variables on their decision-making capacity, using a longitudinal approach from 2009 to 2019 in Brazilian metropolises. By using binomial logistic regression, we analyzed records of discussions, deliberations, and representation patterns in 195 meetings involving 7,759 participants. The results unveil persistent challenges in spaces for institutionalizing deliberative democracy, especially concerning local councils for urban policy implementation, pointing toward renewed research agendas. health education policies field city Brazils Brazil s urbanization scenario decisionmaking decision making investigated 200 201 metropolises regression discussions deliberations 19 7759 7 759 7,75 participants democracy implementation agendas 20 1 775 75 7,7 2 77 7,