Pesquisa | SciELO

A series of biologically active N-acylamino amides were synthesized from readily available amino acids and common fatty acids from oil seeds, including the pharmacophoric group in the family of herbicides propanil, linuron, and diuron. The esterification followed by N-acylation with long-chain fatty acids was carried out with O-(benzotriazole-1-yl)-N,N,N’,N’-tetramethyluronium tetrafluoroborate (TBTU) as activating agent. The N-acylamino esters were synthesized and isolated in good yields (70-98%), which after the hydrolysis step, provided the N-acylamino acids. Finally, new N-acylamino amides were obtained from the reaction with 3,4-dichloroaniline in the presence of TBTU and N,N-diisopropylethylamine (DIPEA), in yields between 24-83%. Bioassays were conducted against arthropod strawberry pests Chaetosiphon fragaefolii, Duponchelia fovealis, and Drosophila suzukii, without any registered compound for use in strawberry crops in Brazil. The bioassay studies showed promising results, with mortality percentages ranging from 20-80%, and the best relative mortality by N-oleyl glycinamide, suggesting a possible fatty chain-amino acid synergistic bioactivity. Overall, this study provides an efficient and sustainable method for synthesizing biologically active N-acylamino amides from abundant natural sources, with potential applications in the development of ecofriendly agrochemicals.

https://doi.org/10.21577/0103-5053.20230135

Anatomopathological changes in laying quails
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NEIVA, DÉBORA C.

; CANDIDO, MATHEUS J. DOS S.

; ASSIS, AMANDA A.

; TAVARES, THAIS G.

; CORREIA, MÁRCIO P.A.

; SOUZA, EMERSON A. DE O.

; DONATELE, DIRLEI M.

; CRUZ, IGOR C.K. DA

; MARTINS, ISABELLA V.F.

; SILVA, MARIA A. DA

; BOELONI, JANKERLE N.

Anais da Academia Brasileira de Ciências

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2024, Volume 96 Nº 2 elocation e20230815

Resumo: En | Texto: En | PDF: En

Abstract The objective of the current study was to carry out a survey of the main anatomopathological alterations in raising quails and evaluate possible interference of these in the bone tissue. To obtain the data, 23 quails were collected from farm in the central Serrana region of Espírito Santo. Necropsies with macroscopic descriptions, microbiological, coproparasitological, radiographic and histomorphometric tests were carried out. It was done data descriptive analysis and average comparision using Student T test. It was found that they presented lesions predominantly in the digestive system, followed by urinary and reproductive, and muscular system, were the altered color of the liver (47%) was the most frequent lesion. In the parasitological exams, it was found oocysts of Eimeira sp. (39.13%). In the microbiological exams, it was detected predominantly Escherichia coli (83%). Moderate osteopenia in quails, but the percentage of trabecular bone on bones was similar between healthy and diseased quails, without bone changes in histology. Microscopically, it was observed lung congestion as predominant lesion. It is concluded that there was predominance of alterations in the digestive system and mild parasitic infection; and although there was moderate level of osteopenia, there wasn’t bone change as a result of the observed infections.

https://doi.org/10.1590/0001-3765202420230815

Análise de Perícias Psicológicas de Trânsito: Um Olhar sobre os Construtos Cognitivos
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Souza, Patrícia Emi de

; Oliveira, Katya Luciane de

; Rueda, Fabián J. M.

; Inácio, Amanda Lays Monteiro

Psico-USF

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Out 2023, Volume 28 Nº 4 Páginas 697 - 710

Resumo: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo O objetivo da pesquisa foi analisar perícias psicológicas realizadas em cidades do Paraná do ano de 2019 no que tange aos aspectos cognitivos. Para tanto, foram consultados 1009 processos arquivados em clínicas credenciadas ao Departamento de Trânsito do Paraná (DETRAN-Pr). Os resultados, inerentes à caracterização das perícias, apontaram que os participantes com idade média de 27 anos, 44,4% possuindo ensino médio completo, 72,2% para a 1ª habilitação, 61% para a categoria A/B. Foram encontrados 12 tipos de instrumentos de Avaliação Psicológica padronizados. Os resultados apontam desempenho médio nos instrumentos e relação de dependência entre os principais construtos investigados. Considera-se que, além de habilidades cognitivas preservadas, a formação dos condutores desde o ensino básico é importante contribuinte para o trânsito seguro.

Abstract The objective of this research was to analyze psychological tests conducted in cities in the state of Paraná in the year 2019, focusing on cognitive aspects. For this purpose, we examined 1009 records from clinics accredited to the State Traffic Department of Paraná (DETRAN-Pr). The results, inherent to the characterization of the expertise, pointed out that the participants had an average age of 27 years, 44.4% had completed secondary education, 72.2% were first-time applicants, and 61% had the A/B category. Twelve types of standardized Psychological Assessment instruments were found. The results indicated that participants exhibited an average performance in these instruments, and there was a significant correlation between the main constructs investigated. These findings suggest that, in addition to maintaining cognitive skills, providing driver education starting from elementary school could significantly contribute to enhancing road safety.

Resumen El objetivo de la investigación fue analizar las pruebas psicológicas realizadas en ciudades de Paraná en el año 2019 sobre aspectos cognitivos. Para ello se consultaron 1009 expedientes archivados en clínicas acreditadas ante el Departamento de Tránsito de Paraná (DETRAN-Pr). Los resultados, inherentes a la caracterización de la especialización, apuntaron que los participantes tenían una edad media de 27 años, 44,4% tenían estudios secundarios completos, 72,2% para el 1º título, 61% para la categoría A/B. Se encontraron doce tipos de instrumentos de Evaluación Psicológica estandarizados. Los resultados muestran un desempeño promedio en los instrumentos y una relación de dependencia entre los principales constructos investigados. Se considera que, además de preservar las habilidades cognitivas, la formación de los conductores desde la escuela primaria en adelante es un importante contribuyente a la seguridad del tráfico.

https://doi.org/10.1590/1413-82712023280404

Functional Outcomes and Satisfaction Rates of Sacral Nerve Stimulation in the Treatment of One and Multiples Pelvic Floor Dysfunctions
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Murad-Regadas, Sthela M

; Holanda, Erico de Carvalho

; Regadas, Carolina M

; Moreira, Amanda Souza

; Fernandes, Larissa M. Camara

; Holanda, Erica Uchoa

; Regadas, Marina Murad

; Cruz, Emanuella C. Murad da

Journal of Coloproctology (Rio de Janeiro)

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Jun 2023, Volume 43 Nº 2 Páginas 104 - 109

Resumo: En | Texto: En | PDF: En

Abstract Objective Few studies have addressed the use of sacral nerve stimulation (SNS) in the treatment of patients with multiple pelvic floor dysfunctions (PFD). So, we evaluated the functional outcomes and level of satisfaction with SNS in selected patients with one or multiples PFD. Methods A prospective database was used to collect information on eligible patients treated for PFD with SNS, and severity of symptoms was assessed with scores and satisfaction rates by visual analogue scale (VAS) at baseline and by the end of follow-up. Results We recruited 70 patients, 98.6% of whom responded positively during the evaluation period (Global Response Assessment ≥ 50% for at least one type of PFD), resulting in the implantation of a permanent SNS device. Additionally, 49 of the patients (71%) had a single PFD (fecal incontinence [FI] = 38; constipation/obstructed defecation syndrome [C/ODS] = 11), while 20 (29%) had more than one PFD (double incontinence/n = 12; double incontinence + C/ODS/n = 8). All scores improved significantly between baseline (pre-SNS) and the end of follow-up (post-SNS), as did VAS in all groups (single and multiple PFD). The pre-SNS scores were higher in patients with a single PFD, including FI (Cleveland clinic Florida incontinence score [CCF-FI]) and C/ODS (Cleveland clinic constipation score [C-CCF] and the Renzi ODS score). The pre-SNS impact of VAS scores was similar in all groups (single and multiple PFD), but the VAS (post-SNS) was significantly lower (better response) for FI alone compared with multiple PFD. Conclusion The SNS technique is an effective and safe option for patients with one or more PFD refractory to conservative measures. Response was positive for at least two PFD, based on reduced correspondent scores and satisfaction rate. (SNS . (PFD) So (VAS followup. followup follow up. up 7 986 98 6 98.6 Global 50 , PFD) device Additionally 4 71% 71 (71% fecal [FI 38 constipationobstructed obstructed CODS C [C/ODS 11, 11 11) 2 29% 29 (29% incontinencen n 12 CODSn 8. 8 8) preSNS pre (pre-SNS postSNS, postSNS post Cleveland CCFFI CCF [CCF-FI] CCCF [C-CCF score. score) (post-SNS better response measures rate (PFD 9 98. 5 (71 3 1 (29 [CCF-FI (7 (2 (

https://doi.org/10.1055/s-0043-1769923

Healthcare workers exposed to COVID-19 patients present an inflammatory status and Th2/Th17/Th22 immune profile: findings from before vaccine application in Brazil COVID19 COVID 19 COVID-1 Th2Th17Th22 ThThTh Th2 Th17 Th22 Th Th2/Th17/Th2 profile COVID1 1 COVID- Th2Th17Th2 Th1 Th2/Th17/Th Th2Th17Th
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AQUINO, RODRIGO CESAR A. DE

; BARROS, BÁRBARA RAFAELA S.

; SILVA, GUILHERME ANTONIO S.

; SOUSA, GEORON F. DE

; SOUZA, EDSON B. DE

; SILVA, DYEGO R.C.

; NASCIMENTO, ARIONE V. DO

; SÁ, IGOR W.A. DE

; LIMA, ELKER L.S. DE

; SILVA, BÁRBARA O.

; LIMA, LUÍSA P.O. DE

; VIEIRA, AMANDA M.

; BARBOSA NETO, ADAUTO G.

; MARCOS, BIANCA F. SÃO

; SILVA, ANNA JÉSSICA D.

; OLIVEIRA, TALITA HELENA ARAÚJO DE

; CARVALHO, BRUNO M.

; MUNIZ, MARIA TEREZA C.

; FREITAS, ANTONIO CARLOS DE

; CAMPELO JÚNIOR, EVÔNIO B.

; MELO, CRISTIANE M.L. DE

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Supl. 1 elocation e20220502

Resumo: En | Texto: En | PDF: En

Abstract Healthcare workers present an increased risk of contagion for the SARS-CoV-2 virus due to their labor exposure. Here, we describe the clinical, laboratory, and immunological characteristics of healthcare workers, before vaccine application, exposed to SARS-CoV-2-infected patients. We collected sociodemographic, clinical, and laboratory information from 50 professionals who worked during the COVID-19 pandemic at the Clinical Hospital of the Northwest in Brazil. The results showed that most workers are women, over 50 years old, and worked as nursing technicians. Approximately 56% of workers were positive for a previous infection by RT-PCR and/or anti-SARS-CoV-2-immunoglobulin tests. Increased levels of hematocrit, neutrophils, NK lymphocytes, and fibrinogen, were found in positive healthcare workers, suggesting a light inflammatory status. The immunological findings showed an increase in IL-17 production and a Th2/Th17/Th22 profile followed by high serology for anti-SARS-CoV-2 IgM and IgG. Those data reveal the importance of studies with healthcare workers to investigate if the continuous exposition to the virus may result in chronic activation of the immune system and/or pulmonary inflammation in this target group. SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- exposure Here clinical application SARSCoV2infected SARSCoVinfected infected patients sociodemographic 5 COVID19 COVID 19 COVID-1 Brazil women old technicians 56 RTPCR RT PCR andor or antiSARSCoV2immunoglobulin antiSARSCoVimmunoglobulin anti immunoglobulin tests hematocrit neutrophils lymphocytes fibrinogen status IL17 IL 17 IL-1 Th2Th17Th22 ThThTh Th2 Th17 Th22 Th Th2/Th17/Th2 antiSARSCoV2 antiSARSCoV anti-SARS-CoV- IgG group SARS-CoV COVID1 1 COVID- IL1 IL- Th2Th17Th2 Th1 Th2/Th17/Th anti-SARS-CoV Th2Th17Th

https://doi.org/10.1590/0001-3765202320220502

Sintomas Cardiopulmonares Pós-COVID-19: Preditores e Características de Imagem de Pacientes após a Alta Hospitalar PósCOVID19 PósCOVID Pós COVID 19 Pós-COVID-19 PósCOVID1 1 Pós-COVID-1 Pós-COVID- Pós-COVID
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Roberto, Kalil-Filho

; Saretta, Roberta

; Franci, André

; Baracioli, Luciano M.

; Galas, Filomena R. B. G.

; Gil, Juliana S.

; Ferino, Amanda

; Giacovone, Camilla

; Oliveira, Isabella

; Souza, Johnatan

; Batista, Vanessa

; Scalabrini Neto, Augusto

; Costa, Livia do Valle

; Ruiz, Amanda Danieleto

; Ledo, Carla B.

; Nascimento, Teresa Cristina D. C.

; Drager, Luciano F.

Arquivos Brasileiros de Cardiologia

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2023, Volume 120 Nº 5 elocation e20220642

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Fundamento A maioria da evidência sobre o impacto da síndrome COVID pós-aguda (PACS, do inglês, post-acute COVID-19 syndrome) descreve sintomas individuais sem correlacioná-los com exames de imagens. Objetivos Avaliar sintomas cardiopulmonares, seus preditores e imagens relacionadas em pacientes com COVID-19 após alta hospitalar. Métodos Pacientes consecutivos, que sobreviveram à COVID-19, foram contatados 90 dias após a alta hospitalar. A equipe de desfechos clínicos (cega quanto aos dados durante a internação) elaborou um questionário estruturado avaliando sintomas e estado clínico. Uma análise multivariada foi realizada abordando a evolução da COVID-19, comorbidades, ansiedade, depressão, e estresse pós-traumático durante a internação, e reabilitação cardíaca após a alta. O nível de significância usado nas análises foi de 5%. Resultados Foram incluídos 480 pacientes (idade 59±14 anos, 67,5% do sexo masculino) que receberam alta hospitalar por COVID-19; 22,3% necessitaram de ventilação mecânica. A prevalência de pacientes com sintomas cardiopulmonares relacionados à PACS (dispneia, cansaço/fadiga, tosse e desconforto no peito) foi de 16,3%. Vários parâmetros de tomografia computadorizada do tórax e de ecocardiograma foram similares entre os pacientes com e sem sintomas cardiopulmonares. A análise multivariada mostrou que sintomas cardiopulmonares foram relacionados de maneira independente com sexo feminino (OR 3,023; IC95% 1,319-6,929), trombose venosa profunda durante a internação (OR 13,689; IC95% 1,069-175,304), nível elevado de troponina (OR 1,355; IC95% 1,048-1,751) e de proteína C reativa durante a internação (OR 1,060; IC95% 1,023-1,097) e depressão (OR 6,110; IC95% 2,254-16,558). Conclusão Os sintomas cardiopulmonares relacionados à PACS 90 dias após a alta hospitalar são comuns e multifatoriais. Além dos marcadores trombóticos, inflamatórios e de lesão miocárdica durante a internação, sexo feminino e depressão foram associados independentemente com sintomas cardiopulmonares relacionados à PACS. Esses resultados destacaram a necessidade de uma abordagem multifacetada direcionada a pacientes susceptíveis. pósaguda pós aguda PACS, (PACS inglês postacute post acute COVID19 19 COVID-1 syndrome correlacionálos correlacioná los consecutivos COVID19, 19, 9 cega clínico comorbidades ansiedade póstraumático traumático 5 5% 48 idade 5914 59 14 59±1 anos 675 67 67,5 masculino 223 22 3 22,3 mecânica dispneia, dispneia (dispneia cansaçofadiga cansaço fadiga cansaço/fadiga peito 163 16 16,3% OR 3,023 3023 023 IC95 IC 1,3196,929, 13196929 1,319 6,929 , 1 319 6 929 1,319-6,929) 13,689 13689 13 689 1,069175,304, 1069175304 1,069 175,304 069 175 304 1,069-175,304) 1,355 1355 355 1,0481,751 10481751 1,048 1,751 048 751 1,048-1,751 1,060 1060 060 1,0231,097 10231097 1,023 1,097 097 1,023-1,097 6,110 6110 110 2,25416,558. 225416558 2,254 16,558 . 2 254 558 2,254-16,558) multifatoriais trombóticos susceptíveis COVID1 COVID- 4 591 59± 67, 22, 16,3 3,02 302 02 IC9 3196 1,3196,929 1319692 1319 1,31 6929 6,92 31 92 1,319-6,929 13,68 1368 68 069175 1,069175,304 106917530 1069 1,06 175304 175,30 06 17 30 1,069-175,304 1,35 135 35 0481 1,0481,75 1048175 1048 1,04 1751 1,75 04 75 1,048-1,75 106 0231 1,0231,09 1023109 1023 1,02 1097 1,09 09 1,023-1,09 6,11 611 11 25416 2,25416,558 22541655 2254 2,25 16558 16,55 25 55 2,254-16,558 16, 3,0 0 1,3196,92 131969 131 1,3 692 6,9 1,319-6,92 13,6 136 06917 1,069175,30 10691753 1,0 17530 175,3 1,069-175,30 1,0481,7 104817 104 1,7 7 1,048-1,7 10 1,0231,0 102310 102 109 1,023-1,0 6,1 61 2541 2,25416,55 2254165 225 2,2 1655 16,5 2,254-16,55 3, 1,3196,9 13196 1, 69 6, 1,319-6,9 13, 0691 1,069175,3 1069175 1753 175, 1,069-175,3 1,0481, 10481 1,048-1, 1,0231, 10231 1,023-1, 2,25416,5 225416 2, 165 2,254-16,5 1,3196, 1,319-6, 1,069175, 106917 1,069-175, 1,0481 1,048-1 1,0231 1,023-1 2,25416, 22541 2,254-16, 1,3196 1,319-6 1,069175 10691 1,069-175 1,048- 1,023- 2,25416 2,254-16 1,319- 1,06917 1,069-17 2,2541 2,254-1 1,0691 1,069-1 2,254- 1,069-

Abstract Background Most of the evidence about the impact of the post-acute COVID-19 Syndrome (PACS) reports individual symptoms without correlations with related imaging. Objectives To evaluate cardiopulmonary symptoms, their predictors and related images in COVID-19 patients discharged from hospital. Methods Consecutive patients who survived COVID-19 were contacted 90 days after discharge. The Clinic Outcome Team structured a questionnaire evaluating symptoms and clinical status (blinded for hospitalization data). A multivariate analysis was performed to address the course of COVID-19, comorbidities, anxiety, depression, and post-traumatic stress during hospitalization, and cardiac rehabilitation after discharge. The significance level was set at 5%. Results A total of 480 discharged patients with COVID-19 (age: 59±14 years, 67.5% males) were included; 22.3% required mechanical ventilation. The prevalence of patients with PACS-related cardiopulmonary symptoms (dyspnea, tiredness/fatigue, cough, and chest discomfort) was 16.3%. Several parameters of chest computed tomography and echocardiogram were similar in patients with and without cardiopulmonary symptoms. The multivariate analysis showed that PACS-related cardiopulmonary-symptoms were independently related to female sex (OR 3.023; 95% CI 1.319-6.929), in-hospital deep venous thrombosis (OR 13.689; 95% CI 1.069-175.304), elevated troponin I (OR 1.355; 95% CI 1.048-1.751) and C-reactive protein during hospitalization (OR 1.060; 95% CI 1.023-1.097) and depression (OR 6.110; 95% CI 2.254-16.558). Conclusion PACS-related cardiopulmonary symptoms 90 days post-discharge are common and multifactorial. Beyond thrombotic and markers of inflammation/myocardial injury during hospitalization, female sex and depression were independently associated with cardiopulmonary-related PACS. These results highlighted the need for a multifaceted approach targeting susceptible patients. postacute post acute COVID19 COVID 19 COVID-1 PACS (PACS imaging hospital 9 discharge blinded data. data . data) COVID19, 19, comorbidities anxiety posttraumatic traumatic 5 5% 48 age (age 5914 59 14 59±1 years 675 67 67.5 males included 223 22 3 22.3 ventilation PACSrelated dyspnea, dyspnea (dyspnea tirednessfatigue tiredness fatigue tiredness/fatigue cough discomfort 163 16 16.3% cardiopulmonarysymptoms OR 3.023 3023 023 95 1.3196.929, 13196929 1.319 6.929 , 1 319 6 929 1.319-6.929) inhospital 13.689 13689 13 689 1.069175.304, 1069175304 1.069 175.304 069 175 304 1.069-175.304) 1.355 1355 355 1.0481.751 10481751 1.048 1.751 048 751 1.048-1.751 Creactive C reactive 1.060 1060 060 1.0231.097 10231097 1.023 1.097 097 1.023-1.097 6.110 6110 110 2.25416.558. 225416558 2.254 16.558 2 254 558 2.254-16.558) postdischarge multifactorial inflammationmyocardial inflammation myocardial cardiopulmonaryrelated COVID1 COVID- 4 591 59± 67. 22. 16.3 3.02 302 02 3196 1.3196.929 1319692 1319 1.31 6929 6.92 31 92 1.319-6.929 13.68 1368 68 069175 1.069175.304 106917530 1069 1.06 175304 175.30 06 17 30 1.069-175.304 1.35 135 35 0481 1.0481.75 1048175 1048 1.04 1751 1.75 04 75 1.048-1.75 106 0231 1.0231.09 1023109 1023 1.02 1097 1.09 09 1.023-1.09 6.11 611 11 25416 2.25416.558 22541655 2254 2.25 16558 16.55 25 55 2.254-16.558 16. 3.0 0 1.3196.92 131969 131 1.3 692 6.9 1.319-6.92 13.6 136 06917 1.069175.30 10691753 1.0 17530 175.3 1.069-175.30 1.0481.7 104817 104 1.7 7 1.048-1.7 10 1.0231.0 102310 102 109 1.023-1.0 6.1 61 2541 2.25416.55 2254165 225 2.2 1655 16.5 2.254-16.55 3. 1.3196.9 13196 1. 69 6. 1.319-6.9 13. 0691 1.069175.3 1069175 1753 175. 1.069-175.3 1.0481. 10481 1.048-1. 1.0231. 10231 1.023-1. 2.25416.5 225416 2. 165 2.254-16.5 1.3196. 1.319-6. 1.069175. 106917 1.069-175. 1.0481 1.048-1 1.0231 1.023-1 2.25416. 22541 2.254-16. 1.3196 1.319-6 1.069175 10691 1.069-175 1.048- 1.023- 2.25416 2.254-16 1.319- 1.06917 1.069-17 2.2541 2.254-1 1.0691 1.069-1 2.254- 1.069-

https://doi.org/10.36660/abc.20220642

GREENHOUSE GAS EMISSIONS AND CHEMICAL AND PHYSICAL SOIL ATTRIBUTES OF OFF-SEASON AGRICULTURAL PRODUCTION SYSTEMS IN THE SAVANNAH OF MARANHÃO STATE, BRAZIL OFFSEASON OFF SEASON STATE
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Brito, Lucélia de C. R. de

; Souza, Henrique A. de

; Deon, Diana S.

; Souza, Ivanderlete M. de

; Santos, Smaiello F. da C. B. dos

; Sobral, Amanda H. S.

Engenharia Agrícola

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2023, Volume 43 Nº 2 elocation e20220181

Resumo: En | Texto: En | PDF: En

ABSTRACT Management of agricultural production systems interferes with greenhouse gases (GHG) emissions, thereby altering physical, chemical, and biological attributes of soil; therefore, it is important to understand the relationship between soil attributes and GHG emissions. This study evaluated GHG emissions and their relationship with soil attributes in off-season soybean, maize, brachiaria and eucalyptus production systems. The experiment was carried out in Brejo, Maranhão, Brazil, with soybean ( Glycine max ), maize ( Zea mays ), brachiaria ( Urochloa ruzizienses ), and eucalyptus ( Eucalyptus grandis ). Fluxes of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) were evaluated using air samples analyzed by gas chromatography. Soil attributes were ammonium and nitrate contents, total organic carbon, moisture, pH, density, total porosity, and water-filled pore space. N2O flux was 287.1 µg m-2 h -1 for eucalyptus cultivation, while areas cultivated with soybeans, maize and brachiaria had influxes of 46.7, 7.2, and 13.17 µg m-2 h-1, respectively. In the off-season, the highest emissions of N2O and CO2 were measured in eucalyptus areas due to soil moisture and porosity conditions provided by accumulation of litter on the soil surface. (GHG physical chemical therefore offseason off season Brejo Maranhão Brazil , ) . CO2, CO (CO2) CH4, CH4 CH (CH4) NO N O (N2O chromatography contents pH density waterfilled water filled space 2871 287 1 287. m2 m 2 m- - cultivation soybeans 467 46 7 46.7 72 7.2 1317 13 17 13.1 h1, h1 1, h-1 respectively offseason, season, surface (CO2 (CH4 28 4 46. 7. 131 13. h- (CO (CH

https://doi.org/10.1590/1809-4430-eng.agric.v43n2e20220181/2023

An interdisciplinary therapy for lifestyle change is effective in improving psychological and inflammatory parameters in women with grade I obesity
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SOUZA, ADRIANA P. DE

; CARVALHO, LORENZA OLIVEIRA T.

; PEDROSO, AMANDA PAULA

; MORAES, AMANDA DE SANTOS

; CIPULLO, MARCOS ALBERTO TADDEO

; DÂMASO, ANA RAIMUNDA

; TELLES, MÔNICA M.

; OYAMA, LILA M.

; TASHIMA, ALEXANDRE K.

; CARANTI, DANIELLE A.

; RIBEIRO, ELIANE B.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Supl. 2 elocation e20230365

Resumo: En | Texto: En | PDF: En

Abstract Obesity and depression, disorders associated with inflammation, have high incidences in women. Understanding the derangements present in the initial phase of obesity may point to factors that could help avoiding disease aggravation. The present study aimed at investigating the effects of a 6-months interdisciplinary therapy for weight loss in women with grade I obesity. Before and after the therapy, 37 middle-aged women donated blood and responded to questionnaires for depression and anxiety symptoms. Inflammatory parameters were evaluated in serum and a preliminary screening of the plasma proteome was performed. The therapy decreased anthropometric, psychological scores, and serum levels of inflammatory parameters. Depression and anxiety scores correlated positively with some inflammatory parameters. The proteomic analysis showed changes in proteins related to cholesterol metabolism and inflammatory response. Interdisciplinary therapy improves anthropometric and inflammatory statuses and ameliorating psychological symptoms. The decrease of MCP-1 levels after interdisciplinary therapy has not been reported so far, at the best of our knowledge. The present demonstration of positive associations of inflammatory markers and psychological scores indicate that these mediators may be useful to monitor psychological status in obesity. The present proteome data, although preliminary, pointed to plasma alterations indicative of improvement of inflammation after interdisciplinary therapy. aggravation 6months months 6 3 middleaged middle aged symptoms performed response MCP1 MCP 1 MCP- far knowledge data

https://doi.org/10.1590/0001-3765202320230365

Lipid profile and reproductive performance of female offspring of SWISS mouse females supplemented with resveratrol or canjiqueira (Byrsonima cydoniifolia A Juss) during gestation Byrsonima Juss
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MENEZES, ADRIANA C. GUERCIO

; BRANDÃO, LORENA S.R.

; PORTUGAL, LUCIANE C.

; MATSUBARA, LIDIA M.

; MAIA, ELAINE MARIA A.

; SAKODA, JHESSICA N.

; PROVIDELO, GILSON A.

; NAVAREZI, AMANDA G.

; SANTOS, KELY CRISTINA N. DOS

; GUIMARÃES, RITA DE CÁSSIA A.

; SOUZA, ALBERT S. DE

; SOUZA, MARIA INÊS L.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Nº 4 elocation e20190804

Resumo: En | Texto: En | PDF: En

Abstract This study aimed to resveratrol supplementation (at 5 or 10 mg/kg) and a hydroethanolic extract of canjiqueira fruits (150 mg/kg) on female SWISS mice. Total cholesterol, high-density lipoprotein (HDL), triglyceride levels, gestation rates, and embryonic implantation rates in their female Offspring was evaluated. In conclusion, the consumption of canjiqueira fruit extract altered the lipid profile of their female offspring, and did not impact their reproductive performance. Supplementing female SWISS mice with 10 mg/kg of resveratrol increased total cholesterol, triglycerides, and HDL levels, thereby enhancing the reproductive efficiency of their offspring. at 1 mgkg mg kg 150 (15 cholesterol highdensity high density HDL, , (HDL) levels evaluated conclusion offspring performance triglycerides 15 (1 (HDL (

https://doi.org/10.1590/0001-3765202320190804

10.

Different stocks of Prochilodus lineatus along the Cinzas River basin: contributions from a free-flowing tributary in the Capivara Reservoir region, Paranapanema River basin (upper Paraná River) freeflowing free flowing region upper
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Ferreira, Dhiego G.

; Galindo, Bruno A.

; Limeira, Daniel M.

; Garla, Alanna

; Marques, Ana J. C.

; Moreira, Amanda A.

; Rodrigues, Maria V. H.

; Clemente, Roberta C.

; Shimpo, Jhennifer Y.

; Martins, Gabriel A.

; Almeida, Fernanda S.

; Apolinário-Silva, Caroline

; Zanatta, Augusto S.

; Sofia, Silvia H.

; Souza-Shibatta, Lenice

Neotropical Ichthyology

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2023, Volume 21 Nº 4 elocation e230080

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Barragens são umas das principais ameaças para peixes potamódromos Neotropicais, causando bloqueios de rotas migratórias e perdas de áreas de reprodução em um número crescente de sistemas de drenagem. Neste cenário, dados atuais defendem que a manutenção de tributários de fluxo livre em bacias hidrográficas fragmentadas seria uma importante estratégia na conservação de peixes migradores, tanto em situações de panmixia quanto em cenários com diferentes estoques coexistindo, tal como já relatado para Prochilodus lineatus. Este estudo analisou locos microssatélites e haplótipos mitocondriais em amostras de P. lineatus obtidas em diferentes anos ao longo da bacia do rio das Cinzas, no reservatório de Capivara (maior reservatório na calha principal do rio Paranapanema, alto rio Paraná) e no rio Tibagi, todos conectados por trechos livres, visando investigar as contribuições da bacia do rio das Cinzas na dinâmica populacional de peixes migradores na região do reservatório de Capivara. Ambos os marcadores detectaram altos níveis de diversidade genética e indicaram diferentes estoques de P. lineatus ao longo da bacia do rio das Cinzas, sugerindo alguma estruturação genética temporal, bem como a mistura destes estoques na amostra do reservatório de Capivara e de outros pontos, corroborando as relações de estoques neste reservatório e seus tributários. Neotropicais drenagem cenário coexistindo P maior Paranapanema Paraná Tibagi livres temporal pontos

Abstract Dams are one of the main threats to Neotropical potamodromous fish, causing blockages of migratory routes and loss of breeding areas in an increasing number of drainage systems. In this scenario, current data argue that the maintenance of free-flowing tributaries along fragmented watersheds would be an important strategy to the migratory fish conservation, both in panmixia situations and in scenarios with different coexisting stocks, as already reported for Prochilodus lineatus. This study analyzed microsatellite loci and mitochondrial haplotypes in samples of P. lineatus obtained in different years along the Cinzas River basin, as well as in the Capivara Reservoir (largest Reservoir in the Paranapanema main channel, upper Paraná River) and in the Tibagi River, all connected by free stretches, aiming to investigate the contributions of the Cinzas River basin to the population dynamics of migratory fish in the Capivara reservoir region. Both markers detected high genetic diversity levels and indicated different genetic stocks of P. lineatus along the Cinzas River basin, suggesting some temporal genetic structuring, as well as the mixture of these stocks in the sample from the Capivara reservoir and from other points, corroborating the relationships among stocks in this reservoir and its tributaries. systems scenario freeflowing flowing conservation P largest channel stretches region structuring points

https://doi.org/10.1590/1982-0224-2023-0080

11.

Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)
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SOUZA-SILVA, GUILHERME ANTONIO DE

; AQUINO, RODRIGO CESAR A. DE

; SOUSA, GEORON F. DE

; BARROS, BÁRBARA RAFAELA S.

; LIMA, MARÍLLIA RAPHAELLA C.F. DE

; CRUZ, LEONARDO C.O.

; MARCOS, BIANCA F. SÃO

; SILVA, ANNA JÉSSICA D.

; TALES, APARECIDA VIRGÍNIA S.

; DINIZ, MADI V.

; LIMA, MÔNICA MARIA C.

; SÁ, IGOR W.A. DE

; RODRIGUES, FÁBIO AUGUSTO C.

; SOUZA, EDSON B. DE

; NASCIMENTO, ARIONE V. DO

; VIEIRA, AMANDA M.

; CARVALHO, BRUNO M. DE

; CAMPELO JÚNIOR, EVÔNIO B.

; FREITAS, ANTONIO CARLOS DE

; MELO, CRISTIANE M.L. DE

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 3 elocation e20211590

Resumo: En | Texto: En | PDF: En

Abstract Health professionals working to mitigate the COVID-19 pandemic are one of the main risk groups for the disease, being prioritized for vaccination. Considering this, the aim of this study was to analyze the immune response of these professionals immunized with CoronaVac in the first and second doses. Blood samples were collected after the first and second doses of the vaccine (CoronaVac) and used to investigate hematological and biochemical parameters, analysis of immunoglobulin production, cytokines, and gene expression profile, as well as the identification of subsets of immune cells. Post-first dose immunological phenotypic memory (CD27+) profiles (T CD4+, TCD8+ and CD19+) showed a significant increase, as did Monocyte APCs (CD80+HLA-DR+) in relation to the second dose. The cytokines IL-2, IL-6 and IFN-° showed increased values in relation to the other analyzed cytokines. The Th2/Th17 profile in the second dose was characterized by gene expression analysis. The production of IgM and IgG after vaccination showed statistically significant values in the comparison between doses. CoronaVac showed activation of APCs monocytes, memory response of T and B lymphocytes, with immunoglobulins production. This set of responses is characterized by the Th2/Th17 immunological profile.

https://doi.org/10.1590/0001-3765202220211590

12.

A novel collagenolytic protease from Mucor subtilissimus UCP 1262: Comparative analysis of production and extraction in submerged and stated-solid fermentation
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SOUZA, KESSIA P.S.

; CUNHA, MÁRCIA N.C.

; BATISTA, JUANIZE M.S.

; OLIVEIRA, VAGNE M.

; NASCIMENTO, THIAGO P.

; CONNIFF, AMANDA E.S.

; COSTA, ROMERO M.P.B.

; PORTO, TATIANA S.

; PORTO, CAMILA S.

; PORTO, ANA LÚCIA F.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 4 elocation e20201438

Resumo: En | Texto: En | PDF: En

Abstract This work aimed to compare the production of collagenolytic proteases produced by M. subtilissimus UCP1262 in submerged fermentation (SF) and solid-state fermentation (SSF) as well as extracting in aqueous two-phase system (ATPS). Collagenolytic protease production was performed in using MS-2 culture medium (SF) and soybean bran as substrate (SSF). Subsequently, the fermented liquid from both fermentations were used for the extraction of enzyme by ATPS, it was verified the influence of different variables from a factorial design 23. In SSF the highest protease and collagenolytic activities were achieved with 362.66 U/mL and 179.81 U/mL, respectively. When compared with SF (26.33 and 18.70 U/mL) higher values were obtained in the activities. The protease partitioning from SF and SSF in ATPS showed a similar profile showing higher affinity for the polymer rich phase. The highest value for the response variable purification factor (3.49) was obtained in the system using SSF. Thus, SSF shows promise as a bioprocess for extracellular production of collagenolytic proteases, using of soybean bran as substrate had used sustainable raw material, aiming application this possible enzyme in the treatment of burns and postoperative scarring.

https://doi.org/10.1590/0001-3765202220201438

13.

Seep hunting in the Santos Basin, Southwest Atlantic: sampling strategy and employed methods of the multidisciplinary cruise BIOIL 1
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Sumida, Paulo Y. G.

; Pellizari, Vivian H.

; Lourenço, Rafael A.

; Signori, Camila N.

; Bendia, Amanda G.

; Carrerette, Orlemir

; Nakamura, Fernanda M.

; Ramos, Raissa B.

; Bergamo, Gilberto

; Souza, Bruno H. M.

; Butarelli, Ana C. A.

; Passos, Júlia G.

; Dias, Rodolfo J. S.

; Maly, Mascimiliano

; Banha, Thomás N. S.

; Güth, Arthur Z.

; Soares, Luiza F.

; Perugino, Paula D. N.

; Santos, Felipe R.

; Santana, Frederico R.

; Mahiques, Michel M. de

Ocean and Coastal Research

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2022, Volume 70 Supl. 2 elocation e22031

Resumo: En | Texto: En | PDF: En

Abstract The continental margin off the southeastern Brazilian coast is punctuated by a series of geological-geomorphological features, such as subsurface saline diapirs and pockmarks at the seafloor interface, which evidence the abundant presence of oil and gas in the region. In several of these sites, hydrocarbons can be naturally released into the water column, areas are cold seep areas. These are marked by the presence of oil- and gas-dependent ecosystems, where specific organisms are able to fix carbon from hydrocarbon chemosynthesis. In addition, light hydrocarbon fluid flow through the sediment may build up authigenic carbonates that can be further colonized by cold-water corals, generating large carbonate mounds over geological time, normally positioned at the border of these pockmark features. The present work reports on a multidisciplinary oceanographic cruise carried out in the Santos Basin, SW Atlantic, to seek, map, and collect geological, chemical, and biological data from different deep-sea habitats. The cruise occurred in November 2019 on the R/V Alpha Crucis of the Oceanographic Institute of the University of São Paulo (IOUSP). We intended to discover and detail different geomorphological features, characterize free-living and symbiotic microorganisms, determine the chemosynthetic rates in relation to heterotrophic microbial production, and characterize the fauna and study their ecological and evolutionary links within and across ocean basins. All discoveries made during the cruise and their respective results will be presented separately in several papers that comprise this special volume.

https://doi.org/10.1590/2675-2824070.22077pygs

14.

Correlations between Web Searches and COVID-19 Epidemiological Indicators in Brazil
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Locatelli, Marcelo Sartori

; Cunha, Evandro L. T. P

; Guiginski, Janaína

; Franco, Ramon A. S

; Bernardes, Tereza

; Alzamora, Pedro Loures

; Silva, Daniel Victor F. da

; Ganem, Marcelo Augusto S

; Santos, Thiago H. M

; Carvalho, Anne I. R

; Souza, Leandro M. V

; Paixão, Gabriela P. F

; Chaves, Elisa França

; Santos, Guilherme Bezerra dos

; Santos, Rafael Vinícius dos

; Freitas, Amanda Cupertino de

; Flores, Matheus G

; Biezuner, Rachel F

; Cardoso, Rodolfo Lins

; Fonseca, Rodrigo Machado

; Silva, Ana Paula Couto da

; Meira Jr, Wagner

Brazilian Archives of Biology and Technology

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2022, Volume 65 elocation e22210648

Resumo: En | Texto: En | PDF: En

Abstract: COVID-19 rapidly spread across the world in an unprecedented outbreak with a massive number of infected and fatalities. The pandemic was heavily discussed and searched on the internet, which generated big amounts of data related to it. This led to the possibility of attempting to forecast coronavirus indicators using the internet data. For this study, Google Trends statistics for 124 selected search terms related to pandemic were used in an attempt to find which keywords had the best Spearman correlations with a lag, as well as a forecasting model. It was found that keywords related to coronavirus testing among some others, such as “I have contracted covid”, had high correlations (≥0.7) with few weeks of lag (≤4 weeks). Besides that, the ARIMAX model using those keywords had promising results in predicting the increase or decrease of epidemiological indicators, although it was not able to predict their exact values. Thus, we found that Google Trends data may be useful for predicting outbreaks of coronavirus a few weeks before they happen, and may be used as an auxiliary tool in monitoring and forecasting the disease in Brazil.

https://doi.org/10.1590/1678-4324-2022210648

15.

COVID-19 in the Perioperative Period of Cardiovascular Surgery: the Brazilian Experience
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Gomes, Walter J.

; Rocco, Isadora

; Pimentel, Wallace S.

; Pinheiro, Aislan H. B.

; Souza, Paulo M. S.

; Costa, Luiz A. A.

; Teixeira, Marjory M. P.

; Ohashi, Leonardo P.

; Bublitz, Caroline

; Begot, Isis

; Moreira, Rita Simone L

; Hossne Jr, Nelson A.

; Vargas, Guilherme F.

; Branco, João Nelson R.

; Teles, Carlos A.

; Medeiros, Eduardo A. S.

; Sáfadi, Camila

; Rampinelli, Amândio

; Moratelli Neto, Leopoldo

; Rosado, Anderson Rosa

; Mesacasa, Franciele Kuhn

; Capriata, Ismael Escobar

; Segalote, Rodrigo Coelho

; Palmieri, Deborah Louize da Rocha Vianna

; Jardim, Amanda Cristina Mendes

; Vianna, Diego Sarty

; Coutinho, Joaquim Henrique de Souza Aguiar

; Jazbik, João Carlos

; Coutinho, Henrique Madureira da Rocha

; Kikuta, Gustavo

; Almeida, Zely Sant'Anna Marotti de

; Feguri, Gibran Roder

; Lima, Paulo Ruiz Lucio de

; Franco, Anna Carolina

; Borges, Danilo de Cerqueira

; Cruz, Felipe Ramos Honorato De La

; Croti, Ulisses Alexandre

; Borim, Bruna Cury

; Marchi, Carlos Henrique De

; Goraieb, Lilian

; Postigo, Karolyne Barroca Sanches

; Jucá, Fabiano Gonçalves

; Oliveira, Fátima Rosane de Almeida

; Souza, Rafael Bezerra de

; Zilli, Alexandre Cabral

; Mas, Raul Gaston Sanchez

; Bettiati Junior, Luiz Carlos

; Tranchesi, Ricardo

; Bertini Jr, Ayrton

; Franco, Leandro Vieira

; Fernandes, Priscila

; Oliveira, Fabiana

; Moraes Jr, Roberto

; Araújo, Thiago Cavalcanti Vila Nova de

; Braga, Otávio Penna

; Pedrosa Sobrinho, Antônio Cavalcanti

; Teixeira, Roberta Tavares Barreto

; Camboim, Irla Lavor Lucena

; Gomes, Eduardo Nascimento

; Reis, Pedro Horigushi

; Garcia, Luara Piovan

; Scorsioni, Nelson Henrique Goes

; Lago, Roberto

; Guizilini, Solange

Brazilian Journal of Cardiovascular Surgery

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Dez 2021, Volume 36 Nº 6 Páginas 725 - 735

Resumo: En | Texto: En | PDF: En

Abstract Introduction: We investigated the clinical course and outcomes of patients submitted to cardiovascular surgery in Brazil and who had developed symptoms/signs of coronavirus disease 2019 (COVID-19) in the perioperative period. Methods: A retrospective multicenter study including 104 patients who were allocated in three groups according to time of positive real time reverse transcriptase-polymerase chain reaction (RT-PCR) for the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2): group 1, patients who underwent cardiac surgery > 10 days after positive RT-PCR; group 2, patients with a positive RT-PCR within 10 days before or after surgery; group 3, patients who presented positive RT-PCR > 10 days after surgery. The primary outcome was mortality and secondary outcomes were postoperative complications, intensive care unit (ICU) length of stay, and postoperative days of hospitalization. Results: The three groups were similar with respect to age, the European System of Cardiac Operative Risk Evaluation score, and comorbidities, except hypertension. Postoperative complications and death were significantly higher in groups 2 and 3 than in group 1, and no significant difference between groups 2 and 3 was seen. Group 2 showed a high prevalence of surgery performed as an urgent procedure. Although no significant differences were observed in ICU length of stay, total postoperative hospitalization time was significantly higher in group 3 than in groups 1 and 2. Conclusion: COVID-19 affecting the postoperative period of patients who underwent cardiovascular surgery is associated with a higher rate of morbidity and mortality. Delaying procedures in RT-PCR-positive patients may help reduce risks of perioperative complications and death.

https://doi.org/10.21470/1678-9741-2021-0960

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