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The essential oil extracted from Anemia tomentosa (EOAT) has shown larvicidal potential against Aedes aegypti, based on methods in vitro and in silico. Chromatographic and spectrometric techniques (gas chromatography-mass spectrometry (CG-MS), gas chromatography-flame ionization detector (GC-FID)), as well as mono and bidimensional nuclear magnetic resonance spectroscopy (NMR) were used to assess 10 essential oil components. Chemical composition of essential oil comprised 87.51% sesquiterpenes, with emphasis on presilphiperfolan-1-ol (42.13%) and silphiperfol-5-ene (19.47%). Larvicidal assay conducted in vitro with EOAT has evidenced potential cytotoxic activity up to 48 h exposure to it; mortality rate observed for A. aegypti larvae exposed to essential oil reached 100%. Study conducted in silico with chemical compounds deriving from the herein investigated plant species has evidenced its potential to inhibit acetylcholinesterase in A. aegypti. Activity of triquinane sesquiterpenes ranging from -6.8 to -8.2 kcal mol-1 stood out in comparison to that of temephos (-7.5 kcal mol-1). Chemical compounds identified in the investigated essential oil presented low human and environmental toxicity, as observed in absorption, distribution, metabolism and excretion, and toxicity (ADMETox) predictions. (EOAT chromatographymass chromatography mass CGMS, CGMS CG MS , (CG-MS) chromatographyflame flame GCFID, GCFID GC FID (GC-FID)) NMR (NMR 1 components 8751 87 51 87.51 presilphiperfolan1ol presilphiperfolanol presilphiperfolan ol 42.13% 4213 42 13 (42.13% silphiperfol5ene silphiperfolene silphiperfol 5 ene 19.47%. 1947 19.47% . 19 47 (19.47%) 4 it A 100 100% 6.8 68 6 8 -6. 8.2 82 2 -8. mol1 mol mol- 7.5 75 7 (-7. mol1. mol-1) absorption distribution excretion ADMETox (ADMETox predictions (CG-MS (GC-FID) 875 87.5 42.13 421 (42.13 194 19.47 (19.47% 6. -6 8. -8 7. (-7 (GC-FID 87. 42.1 (42.1 19.4 (19.47 - (- 42. (42. 19. (19.4 ( (42 (19. (4 (19 (1

https://doi.org/10.21577/0103-5053.20240127

In vitro and in silico Antifungal Activity of Metabolites from Bredemeyera brevifolia (Benth.) Klotzsch ex A.W. Benn. Benth. Benth (Benth. AW A W A.W Benn (Benth
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Barreto, Rubens S.

; Costa, Diego M. da

; Sousa, Jailan S.

; Jesus, Vilisaimon S. de

; Silva, Agnaldo P. da

; Buonafina-Paz, Maria Daniela S.

; Santos, Franz A. G. dos

; Neves, Rejane P.

; Pastore, José F. B.

; Andrade, Bruno S.

; Soares, Wagner R. A.

; Brandão, Hugo N.

; Aguiar, Rosane M.

; Alves, Clayton Q.

Journal of the Brazilian Chemical Society

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2025, Volume 36 Nº 3 elocation e-20240158

Resumo: En | Texto: En | PDF: En

Bredemeyera Willd. is a genus of the Polygalaceae family, occurring in Brazil. This research aims to evaluate the phytochemical and antifungal potential, in vitro and in silico, of Bredemeyera brevifolia extracts. The identification of fatty acids by gas chromatography-mass spectrometry and their quantification by gas chromatography-flame ionization detector showed that palmitic (36.55 mg g−1), margaric (3.66 mg g−1), linolelaidic (108.82 mg g−1), oleic (7.86 mg g−1), stearic (41.11 mg g−1), dihomo-γ-linolenic (85.48 mg g−1), and arachidic (209.00 mg g−1) acids are present in the hexane extract. From the chloroform extract, 1-hydroxy-3,7-dimethoxyxanthone was isolated and identified. The minimum inhibitory concentration assay with methanolic extract demonstrated antifungal activity against species of the genus Candida, Sporothrix, and Trichophyton for up to 96 h, with values ranging from 64-1024 μg mL−1. The in silico study with the chemical compounds of this species showed fungal inhibition potential of sterol-14-α-demethylase, highlighting 1-hydroxy-3,7-dimethoxyxanthone, which presented an affinity energy of -8.8 kcal mol−1, greater than that observed for fluconazole (−8.3 kcal mol−1). The fatty acids interacted with the active site of the enzyme but presented energies ≥ −7.4 kcal mol−1. Compounds isolated from this species have pharmacokinetic potential for the development of pharmaceutical formulations. Willd family Brazil extracts chromatographymass chromatography mass chromatographyflame flame 36.55 3655 36 55 (36.5 g−1, g1 g g−1 , 1 3.66 366 3 66 (3.6 108.82 10882 108 82 (108.8 7.86 786 7 86 (7.8 41.11 4111 41 11 (41.1 dihomoγlinolenic dihomo γ linolenic 85.48 8548 85 48 (85.4 209.00 20900 209 00 (209.0 1hydroxy3,7dimethoxyxanthone 1hydroxy37dimethoxyxanthone hydroxydimethoxyxanthone hydroxy 3,7 dimethoxyxanthone identified Candida Sporothrix 9 h 641024 64 1024 64-102 mL1 mL mL−1 sterol14αdemethylase, sterol14αdemethylase sterolαdemethylase sterol 14 α demethylase, demethylase sterol-14-α-demethylase 1hydroxy3,7dimethoxyxanthone, dimethoxyxanthone, 8.8 88 8 -8. mol1 mol mol−1 −8.3 83 (−8. . mol−1) 74 4 −7. formulations 36.5 365 5 (36. g− 3.6 6 (3. 108.8 1088 10 (108. 7.8 78 (7. 41.1 411 (41. 85.4 854 (85. 209.0 2090 20 0 (209. 1hydroxy3 7dimethoxyxanthone 37 3, 64102 102 64-10 mL− αdemethylase 8. -8 mol− −8. (−8 −7 36. (36 3. (3 108. (108 7. (7 41. (41 85. (85 209. 2 (209 1hydroxy 6410 64-1 - −8 (− − ( (10 (4 (8 (20 641 64- (1 (2

https://doi.org/10.21577/0103-5053.20240158

Synthesis, Characterization, and Application of Waste Material Made Magnetic Nanoparticles for the Recovery of Adulterated Markings on Metallic Surfaces Synthesis Characterization
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Souza, Daniel B.

; Silva, Bruno G.

; Sommer, Rubem L.

; Soares, Iane A.

; Pereira, Gabriela R.

; Dornellas, Rafael M.

; Pacheco, Wagner Felippe

Journal of the Brazilian Chemical Society

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2025, Volume 36 Nº 3 elocation e-20240162

Resumo: En | Texto: En | PDF: En

Serial numbers are marks present on several day-to-day objects, created by manufacturers for purposes such as quality control, product tracking and to prevent counterfeiting. In the case of firearms, these characters can be obliterated or damaged by natural processes or intentionally by criminals to prevent positive identification of stolen property. One non-destructive method employed by crime laboratories for the restoration of obliterated characters is the magnetic particle method. The present work aims to study the synthesis and application of waste-made magnetic nanoparticles in the recovery of adulterated markings. A set of analytical-grade materials and metallic waste magnetic nanoparticles were synthesized for comparison purposes. Chemical characterization tests by X-ray fluorescence, morphological tests by scanning electron microscopy (SEM), and magnetization tests by vibrating sample magnetometer (VSM) were performed. The efficacy of the synthesized nanoparticles was tested on 1020 steel plates previously engraved and adulterated by sanding. Both homemade magnetic nanoparticles demonstrated superior performance compared to commercial magnetic particles. The application of homemade magnetic particles, especially using waste as the source of production, decreases the cost of the material and is environmentally friendly. The development of this material makes magnetic serial number restoration a better option for widespread use in forensic procedures. daytoday day objects control counterfeiting firearms property nondestructive non destructive wastemade made markings analyticalgrade analytical grade Xray X ray fluorescence SEM, SEM , (SEM) VSM (VSM performed 102 sanding particles production friendly procedures (SEM 10 1

https://doi.org/10.21577/0103-5053.20240162

Challenges of Multidrug-resistant New Delhi Metallo-beta-Lactamase (NDM-1)-producing Enterobacteriaceae in Kidney Transplant Patients Multidrugresistant Multidrug resistant MetallobetaLactamase Metallo beta Lactamase NDM1producing NDMproducing NDM 1 producing
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Foresto, Renato Demarchi

; Menezes, Lucas Marengo

; Nishimura, Laura Tomoko

; Cristelli, Marina Pontello

; Viana, Laila Almeida

; Santos, Daniel Wagner de Castro Lima

; Requião-Moura, Lúcio R.

; Tedesco-Silva, Helio

; Medina-Pestana, Jose

Brazilian Journal of Nephrology

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Mar 2023, Volume 45 Nº 1 Páginas 116 - 120

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Background: The emergence of multidrug-resistant NDM-1-producing enterobacteriaceae strains has become a threat to inpatients, especially to immunosuppressed ones, such as kidney transplant recipients. NDM-1 is a carbapenemase that makes gram-negative bacteria resistant to many types of antibiotics. The incidence of carbapenemase-producing enterobacteria infection in solid organ transplant recipients is around 3 to 10%, with a mortality rate of up to 30%. Methods: We present a case series of 4 patients with NDM-1-producing enterobacteria isolated in urine cultures or rectal swabs. We also conducted a cross-sectional study 30 days after patient identification, collecting surveillance cultures (rectal swab) from all inpatients to assess the extent of spread of this resistance mechanism; a total of 101 patients were included. Results: Two patients were adequately treated with negative control cultures. The other two patients were not treated because they were asymptomatic and had subsequent negative urine cultures. No new colonization was identified in the cross-sectional screening, and no new cases of urinary NDM-1 infection were recorded after a 4-year follow-up. Conclusion: Surveillance for infections caused by multidrug-resistant strains in hospitals treating immunosuppressed patients should be continued and prompt action should be taken in cases of outbreaks of multidrug-resistant infections. Background multidrugresistant multidrug NDM1producing NDMproducing NDM 1 producing ones NDM1 NDM- gramnegative gram antibiotics carbapenemaseproducing 10 10% 30% Methods swabs crosssectional cross sectional identification swab mechanism included Results screening 4year year followup. followup follow up. follow-up Conclusion

Resumo Histórico: O surgimento de cepas multirresistentes de enterobacteriaceae produtoras de NDM-1 tornou-se uma ameaça para pacientes hospitalizados, especialmente para os imunossuprimidos, como os receptores de transplante renal. NDM-1 é uma carbapenemase que torna as bactérias gram-negativas resistentes a muitos tipos de antibióticos. A incidência de infecção por enterobactérias produtoras de carbapenemas em receptores de transplante de órgãos sólidos é de cerca de 3 a 10%, com uma taxa de mortalidade de até 30%. Métodos: Apresentamos uma série de casos de 4 pacientes com enterobactérias produtoras de NDM-1 isoladas em culturas de urina ou esfregaços retais. Também realizamos um estudo transversal 30 dias após a identificação do paciente, coletando culturas de vigilância (esfregaço retal) de todos os pacientes internados para avaliar a extensão de disseminação deste mecanismo de resistência; foram incluídos um total de 101 pacientes. Resultados: Dois pacientes foram tratados adequadamente com culturas de controle negativo. Os outros dois pacientes não foram tratados porque eram assintomáticos e tiveram culturas de urina negativas subsequentes. Não foi identificada nenhuma nova colonização na triagem transversal, e não foram registrados novos casos de infecção urinária por NDM-1 após um acompanhamento de 4 anos. Conclusão: A vigilância de infecções causadas por cepas multirresistentes em hospitais que tratam pacientes imunossuprimidos deve ser continuada e devem ser tomadas medidas imediatas em casos de surtos desses tipos de infecções. Histórico NDM1 NDM 1 NDM- tornouse tornou se hospitalizados renal gramnegativas gram antibióticos 10 10% 30% Métodos retais paciente esfregaço retal resistência Resultados negativo subsequentes anos Conclusão

https://doi.org/10.1590/2175-8239-jbn-2021-0033

Influence of irrigation and supplementation on performance and ingestive behavior of beef cattle on mixed grass pastures
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Bones, Everton Robert

; Paris, Wagner

; Costa, Olmar Antônio Denardin

; Paula, Adalberto Luiz de

; Belli, Vanesa Pitro

; Neves, Ana Carla da Silva

; Menezes, Luis Fernando Glasenapp de

Revista Brasileira de Zootecnia

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2023, Volume 52 elocation e20220055

Resumo: En | Texto: En | PDF: En

ABSTRACT The purpose of this study was to evaluate the performance of beef cattle with two levels of energy-protein supplementation of low-consumption on African Bermudagrass pasture, overseeded in winter with oat and ryegrass, with or without irrigation. Twenty-four castrated Angus steers (11 months old and had an initial average of 220 kg body weight (BW)) were used in experimental area of 3.6 ha. The experiment was completely randomized in a 2×2 factorial design, with three replicates. The evaluation period was 249 days (July/2019 to March/2020). The treatments were: irrigated pasture with supplementation of 1 g/kg BW or 2.7 g/kg BW and non-irrigated pasture with 1 g/kg BW of supplement or 2.7 g/kg BW. The grazing method was continuous with a variable stocking rate. Irrigation provided pastures with better chemical composition in winter and spring. Irrigation increased the daily accumulation rate in winter (84.6 vs. 45.9 kg DM/ha/day), providing a greater stocking rate (1,702 vs. 1,385 kg/ha) and, consequently, body weight gain per hectare. Supplementation of 2.7 g/kg BW provided a greater stocking rate in winter (1,652 vs. 1,435 kg/ha) and spring (3,096 vs. 2,811 kg/ha), not changing in summer. The association of irrigation and supplementation of 2.7 vs. 1.0 g/kg BW improves the intake pattern by the animal in summer without changing productivity parameters. Irrigation increases productivity and the nutritional value of pasture with higher livestock production per area in periods of water deficit. The supply of 2.7 vs. 1.0 g/kg BW provides a greater stocking rate and body weight gain per hectare. energyprotein energy protein lowconsumption low consumption ryegrass Twentyfour Twenty four 11 (1 22 (BW) 36 3 6 3. ha 2 2× design replicates 24 July/2019 July2019 July 2019 (July/201 March/2020. March2020 March March/2020 . 2020 March/2020) gkg g 27 7 2. nonirrigated non 84.6 846 84 (84. vs 459 45 9 45. DM/ha/day, DMhaday DM/ha/day , DM day DM/ha/day) 1,702 1702 702 (1,70 1385 385 1,38 kg/ha kgha consequently hectare 1,652 1652 652 (1,65 1435 435 1,43 3,096 3096 096 (3,09 2811 811 2,81 kg/ha, 10 0 1. parameters deficit ( (BW July/201 July201 201 (July/20 March202 March/202 202 84. 8 (84 4 1,70 170 70 (1,7 138 38 1,3 1,65 165 65 (1,6 143 43 1,4 3,09 309 09 (3,0 281 81 2,8 July/20 July20 20 (July/2 March20 March/20 (8 1,7 17 (1, 13 1, 1,6 16 14 3,0 30 (3, 28 2, July/2 July2 (July/ March2 March/2 3, (3 July/ (July March/

https://doi.org/10.37496/rbz5220220055

Particularidades dos Pacientes com Arterite de Takayasu em Idade Mais Avançada: Estudo Coorte, Retrospectivo e Transversal Avançada Coorte
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Oliveira, João Calvino Soares de

; Santos, Alexandre Moura dos

; Aguiar, Mariana Freitas de

; Gonçalves Junior, Jucier

; Souza, Alexandre Wagner Silva de

; Pereira, Rosa Maria R.

; Shinjo, Samuel Katsuyuki

Arquivos Brasileiros de Cardiologia

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2023, Volume 120 Nº 1 elocation e20220463

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Background Few studies have assessed elderly patients with Takayasu’s arteritis (TAK). Objectives To evaluate the progression of TAK in different age groups and its possible effects on drug treatment and disease activity. Methods This cross-sectional and retrospective cohort study included 66 TAK patients. Patients were interviewed and data of the 12 preceding months were collected from electronic medical records. The patients were divided into four quartiles according to current age and compared for clinical and laboratory data, treatment, comorbidities, disease status, and functional status. Statistical significance was set at p<0.05. Results The groups were Q1(22-36 years, n=16), Q2(37-42 years, n=18), Q3(43-49 years, n=17), and Q4(51-66 years, n=15). The frequency of patients with disease activity, fatigue, comorbidities and vascular impairments, and the TAK disease extent index were also comparable between the groups. With age, disease duration was longer (p=0.001), fewer patients used prednisone (Q1:43.8%, Q2:33.3%, Q3:11.8%, and Q4:6.7%; p=0.049) and immunosuppressive drugs [Q1:100.0%, Q2:66.7%, Q3:58.8%, and Q4:46.7%; Q1 versus Q3 (p=0.043), and Q1 versus Q4 (p=0.005) in post-hoc analyses], and patients had greater functional status impairment (Q2 versus Q3, p=0.003). In addition, the levels of disease damage, new TAK symptoms, and complications in the preceding 12 months were not different between the groups. Conclusions Older patients with TAK require minimal drug treatment, and have greater impairment of functional status, which may be attributed to aging-related factors. Takayasus Takayasu s TAK. . (TAK) activity crosssectional cross sectional 6 1 records p005 p 0 05 p<0.05 Q12236 Q 22 36 Q1(22-3 years n=16, n16 n n=16 , 16 n=16) Q23742 Q2 37 42 Q2(37-4 n=18, n18 n=18 18 n=18) Q34349 43 49 Q3(43-4 n=17, n17 n=17 17 n=17) Q45166 51 Q4(51-6 n=15. n15 n=15 15 n=15) fatigue impairments p=0.001, p0001 p=0.001 001 (p=0.001) Q143.8%, Q1438 43.8%, 8 (Q1:43.8% Q233.3%, Q2333 33.3%, 33 3 Q2:33.3% Q311.8%, Q3118 11.8%, 11 Q3:11.8% Q46.7% Q467 6.7% 7 Q4:6.7% p=0.049 p0049 049 Q1100.0%, Q11000 100.0%, 100 [Q1:100.0% Q266.7%, Q2667 66.7%, Q2:66.7% Q358.8%, Q3588 58.8%, 58 Q3:58.8% Q446.7% Q4467 46.7% 46 Q4:46.7% p=0.043, p0043 p=0.043 043 (p=0.043) p=0.005 p0005 005 (p=0.005 posthoc post hoc analyses, analyses analyses] (Q p=0.003. p0003 p=0.003 003 p=0.003) addition damage symptoms agingrelated aging related factors (TAK p00 p<0.0 Q1223 2 Q1(22- n1 n=1 Q2374 4 Q2(37- Q3434 Q3(43- Q4516 5 Q4(51- p000 p=0.00 00 (p=0.001 Q143 Q143.8% 438 43.8% (Q1:43.8 Q233 Q233.3% 333 33.3% Q2:33.3 Q311 Q311.8% 118 11.8% Q3:11.8 Q46 Q46.7 67 6.7 Q4:6.7 p=0.04 p004 04 Q1100 Q1100.0% 1000 100.0% 10 [Q1:100.0 Q266 Q266.7% 667 66.7% Q2:66.7 Q358 Q358.8% 588 58.8% Q3:58.8 Q446 Q446.7 467 46.7 Q4:46.7 (p=0.043 (p=0.00 p0 p<0. Q122 Q1(22 n= Q237 Q2(37 Q343 Q3(43 Q451 Q4(51 p=0.0 Q14 Q143.8 43.8 (Q1:43. Q23 Q233.3 33.3 Q2:33. Q31 Q311.8 11.8 Q3:11. Q46. 6. Q4:6. Q110 Q1100.0 100.0 [Q1:100. Q26 Q266.7 66.7 Q2:66. Q35 Q358.8 58.8 Q3:58. Q44 Q446. 46. Q4:46. (p=0.04 (p=0.0 p<0 Q12 Q1(2 Q2(3 Q34 Q3(4 Q45 Q4(5 p=0. Q143. 43. (Q1:43 Q233. 33. Q2:33 Q311. 11. Q3:11 Q4:6 Q11 Q1100. 100. [Q1:100 Q266. 66. Q2:66 Q358. 58. Q3:58 Q4:46 (p=0. p< Q1( Q2( Q3( Q4( p=0 (Q1:4 Q2:3 Q3:1 Q4: [Q1:10 Q2:6 Q3:5 Q4:4 (p=0 p= (Q1: Q2: Q3: [Q1:1 (p= (Q1 [Q1: (p [Q1 [Q

Resumo Fundamentos Poucos estudos avaliaram pacientes idosos com Arterite de Takayasu (AT). Objetivo Avaliar o progresso de AT em diferentes grupos etários em seus possíveis efeitos sobre o tratamento medicamentoso e atividade da doença. Métodos este estudo transversal, retrospectivo, do tipo coorte incluiu 66 pacientes com AT. Os pacientes foram entrevistados, e dados dos 12 meses anteriores foram coletados dos prontuários médicos eletrônicos. Os pacientes foram divididos em quatro quartis de acordo com idade atual, e comparados quanto aos dados clínicos e laboratoriais, tratamento, comorbidades, status da doença, e status funcional. Um p<0,05 foi estabelecido como estatisticamente significativo. Resultados Os grupos foram definidos como Q1(22-36 anos, n=16), Q2(37-42 anos, n=18), Q3(43-49 anos, n=17), e Q4(51-66 anos, n=15). A frequência de pacientes com atividade da doença, fadiga, comorbidades e comprometimentos vasculares, e o índice de extensão da doença (DEI. Tak) foram comparáveis entre os grupos. Pacientes com idade mais avançada apresentaram maior duração da doença (p=0,001) e maior comprometimento do status funcional (Q2 versus Q3, p=0,003); menos pacientes usaram prednisona (Q1:43,8%; Q2:33,3%; Q3:11,8%; e Q4:6,7%; p=0,049) e agentes imunossupressores [Q1:100,0%; Q2:66,7%; Q3:58,8% e Q4:46,7%; Q1 versus Q3 (p=0,043) e Q1 versus Q4 (p=0,005) nas análises post-hoc]. Além disso, os níveis de danos da doença, sintomas de uma nova ocorrência de AT, e complicações nos 12 meses precedentes não foram diferentes entre os grupos. Conclusão Pacientes com AT e idade mais avançada requerem mínima intervenção medicamentosa e apresentam maior comprometimento no status funcional, o que pode ser atribuído a fatores relacionados ao envelhecimento. . (AT) transversal retrospectivo 6 entrevistados 1 eletrônicos atual laboratoriais p005 p 0 05 p<0,0 significativo Q12236 Q 22 36 Q1(22-3 anos n=16, n16 n n=16 , 16 n=16) Q23742 Q2 37 42 Q2(37-4 n=18, n18 n=18 18 n=18) Q34349 43 49 Q3(43-4 n=17, n17 n=17 17 n=17) Q45166 51 Q4(51-6 n=15. n15 n=15 15 n=15) fadiga vasculares DEI. DEI (DEI Tak p=0,001 p0001 001 (p=0,001 (Q p=0,003 p0003 003 p=0,003) Q143,8% Q1438 43,8% 8 (Q1:43,8% Q233,3% Q2333 33,3% 33 3 Q2:33,3% Q311,8% Q3118 11,8% 11 Q3:11,8% Q46,7% Q467 6,7% 7 Q4:6,7% p=0,049 p0049 049 Q1100,0% Q11000 100,0% 100 [Q1:100,0% Q266,7% Q2667 66,7% Q2:66,7% Q358,8% Q3588 58,8% 58 Q3:58,8 Q446,7% Q4467 46,7% 46 Q4:46,7% p=0,043 p0043 043 (p=0,043 p=0,005 p0005 005 (p=0,005 posthoc. posthoc post hoc post-hoc] disso envelhecimento (AT p00 p<0, Q1223 2 Q1(22- n1 n=1 Q2374 4 Q2(37- Q3434 Q3(43- Q4516 5 Q4(51- p=0,00 p000 00 (p=0,00 Q143 Q143,8 438 43,8 (Q1:43,8 Q233 Q233,3 333 33,3 Q2:33,3 Q311 Q311,8 118 11,8 Q3:11,8 Q46 Q46,7 67 6,7 Q4:6,7 p=0,04 p004 04 Q1100 Q1100,0 1000 100,0 10 [Q1:100,0 Q266 Q266,7 667 66,7 Q2:66,7 Q358 Q358,8 588 58,8 Q3:58, Q446 Q446,7 467 46,7 Q4:46,7 (p=0,04 post-hoc p0 p<0 Q122 Q1(22 n= Q237 Q2(37 Q343 Q3(43 Q451 Q4(51 p=0,0 (p=0,0 Q14 Q143, 43, (Q1:43, Q23 Q233, 33, Q2:33, Q31 Q311, 11, Q3:11, Q46, 6, Q4:6, Q110 Q1100, 100, [Q1:100, Q26 Q266, 66, Q2:66, Q35 Q358, 58, Q3:58 Q44 Q446, 46, Q4:46, p< Q12 Q1(2 Q2(3 Q34 Q3(4 Q45 Q4(5 p=0, (p=0, (Q1:43 Q2:33 Q3:11 Q4:6 Q11 [Q1:100 Q2:66 Q3:5 Q4:46 Q1( Q2( Q3( Q4( p=0 (p=0 (Q1:4 Q2:3 Q3:1 Q4: [Q1:10 Q2:6 Q3: Q4:4 p= (p= (Q1: Q2: [Q1:1 (p (Q1 [Q1: [Q1 [Q

https://doi.org/10.36660/abc.20220463

Germination and in vitro development of mature zygotic embryos and protein profile of seedlings of wild and cultivated Hevea brasiliensis
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CARVALHO, JOSIANE C. DE

; NASCIMENTO, GLEISSON DE O.

; SILVA, ANA CLAUDIA L. DA

; FERREIRA, MARIA DAS GRACAS R.

; ARAÚJO, WAGNER L.

; GONÇALVES, JOSÉ FRANCISCO DE C.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 4 elocation e20200515

Resumo: En | Texto: En | PDF: En

Abstract The main factors governing Hevea brasiliensis germination and seedling establishment remains unclear. We examined the effect of growth regulators Indole 3-Acetic Acid (IAA) and 6-Benzylaminopurine (BAP), and their interactions on germination and the development of mature zygotic embryos (MZE) and protein profile of Hevea brasiliensis seedlings from wild and cultivated (clone PB 250) genotypes. Embryonic axes excised from seeds (wild and clone PB 250) were inoculated in Murashige and Skoog medium (control) and supplemented with IAA (3 µM) and BAP (6 µM) individually and their combination (3 µM IAA + 6 µM BAP). For both genotypes, the mature embryos displayed a high percentage of germination and establishment, and the seedlings were characterized by protein bands ranging from 7 to 30 kDa. Notably, the wild genotype showed proteins in the 14 kDa range, which may be associated with one of the major rubber elongation factors (REF). The wild and clone genotypes presented different behavior and strategies in relation to the protein profile in the presence of different growth regulators. Although the latex biosynthetic pathway and its mechanisms of regulation still remain largely unknown, our results aid in our understanding of the dynamics of proteins in different rubber tree clones in vitro.

https://doi.org/10.1590/0001-3765202220200515

Compatibility by a Nonisothermal Kinetic Study of Azathioprine Associated with Usual Excipients in the Product Quality Review Process
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Silva, Carolina R. G.

; Fialho, Silvia L.

; Barbosa, Jamile

; Araújo, Bárbara C. R.

; Carneiro, Guilherme

; Sebastião, Rita C. O.

; Mussel, Wagner N.

; Yoshida, Maria I.

; Freitas-Marques, Maria B. de

Journal of the Brazilian Chemical Society

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Mar 2021, Volume 32 Nº 3 Páginas 638 - 651

Resumo: En | Texto: En | PDF: En

Azathioprine is an immunosuppressive drug for several inflammatory disorders. Due to its clinical relevance, to explore the solid-state properties for excipient compatibility in the product quality review process is essential. Fourier transform infrared spectroscopy, powder X-ray diffraction and thermal analysis (thermogravimetry/derivative thermogravimetry (TG/DTG), differential thermal analysis (DTA), and differential scanning calorimetry (DSC)) were applied. The compatibility studies evidenced that starch pregelatinized, colloidal silicon dioxide, and talc are fully compatible with azathioprine. However, stearic acid, magnesium stearate, and mannitol are incompatible after heat supply at temperatures easily reached by industrial processing. The nonlinear Vyazovkin isoconversional treatment performed the kinetic study of the thermal degradation. The activation energies were determined to clarify the influence of each excipient on the thermal drug stability, an essential procedure in the pharmaceutical development, and all over the commercial live span, in Good Manufacturing Practices.

https://doi.org/10.21577/0103-5053.20200218 201 downloads

Increased modifiable cardiovascular risk factors in patients with Takayasu arteritis: a multicenter cross-sectional study
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Santos, Alexandre Moura dos

; Misse, Rafael Giovani

; Borges, Isabela Bruna Pires

; Gualano, Bruno

; Souza, Alexandre Wagner Silva de

; Takayama, Liliam

; Pereira, Rosa Maria R.

; Shinjo, Samuel Katsuyuki

Advances in Rheumatology

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2021, Volume 61 elocation 1

Resumo: En | Texto: En | PDF: En

Abstract Background: Modifiable cardiovascular risk factors (MCRFs), such as those related to aerobic capacity, muscle strength, physical activity, and body composition, have been poorly studied in Takayasu arteritis (TAK). Therefore, the aim of the study was to investigate MCRFs and their relationships with disease status and comorbidities among patients with TAK. Methods: A multicenter cross-sectional study was conducted between 2019 and 2020, in which 20 adult women with TAK were compared with 16 healthy controls matched by gender, age, and body mass index. The following parameters were analyzed: aerobic capacity by cardiopulmonary test; muscle function by timed-stands test, timed up-and-go test, and handgrip test; muscle strength by one-repetition maximum test and handgrip test; body composition by densitometry; physical activity and metabolic equivalent by IPAQ, quality of life by HAQ and SF-36; disease activity by ITAS2010 and NIH score; and presence of comorbidities. Results: Patients with TAK had a mean age of 41.5 (38.0-46.3) years, disease duration of 16.0 (9.5-20.0) years, and a mean BMI of 27.7±4.5 kg/m2. Three out of the 20 patients with TAK had active disease. Regarding comorbidities, 16 patients had systemic arterial hypertension, 11 had dyslipidemia, and two had type 2 diabetes mellitus, while the control group had no comorbidities. TAK had a significant reduction in aerobic capacity (absolute and relative VO2 peak), muscle strength in the lower limbs, increased visceral adipose tissue, waist-to-hip ratio, reduced walking capacity, decreased weekly metabolic equivalent, and quality of life (P< 0.05) as compared to controls. However, there were no correlations between these MCRFs parameters and disease activity. Conclusions: TAK show impairment in MCRFs; therefore, strategies able to improve MCRF should be considered in this disease.

https://doi.org/10.1186/s42358-020-00157-1 326 downloads

10.

COMVC-19: A Program to protect healthcare workers’ mental health during the COVID-19 Pandemic. What we have learned
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Fukuti, Pedro

; Uchôa, Caroline Louise Mesquita

; Mazzoco, Marina Flaborea

; Cruz, Isabella D'Andrea Garcia da

; Echegaray, Mariana V.F.

; Humes, Eduardo de Castro

; Silveira, Júlia Belizário

; Santi, Talita Di

; Miguel, Euripedes Constantino

; Corchs, Felipe

; Fatori, Daniel

; Campello, Guilherme

; Oliveira, Gabriel M. de

; Argolo, Felipe C.

; Ferreira, Felipe de M.

; Machado, Gustavo

; Argeu, Adriana

; Oliveira, Graça Maria Ramos de

; Serafim, Antônio de Pádua

; Siqueira, Luciana de Lima

; Rossi, Luciane de

; Rios, Izabel Cristina

; Oliveira, Talita Rodrigues de

; Antoniazzi, Leilane C. K.

; Gagliotti, Daniel Augusto Mori

; Abelama Neto, Emílio

; Oliveira Junior, Paulo Novais de

; Correia, Aline Villalobo

; Gonçalves, Luca Schilling

; Tortato, Liana Silva

; Busato, Wagner Machado Moraes

; Guimarães-Fernandes, Flávio

; Alves, Marcos

; Leite Netto, Oswaldo Ferreira

; Schoueri, Patrícia de Campos Lindenberg

; Roque, Márcio de Assis

; Merlin, Silvia Stahl

; Boer, Giovana Cardoso Machado

; Sallet, Paulo Clemente

; Malbergier, André

; Spedo, Mariana Abrahão

; Kamitsuji, Carla Satie

; Faria, Elizabeth de

; Moreira, Moacyr Vergara de Godoy

; Kaufman, Arthur

; Abdo, Carmita

; Scanavino, Marco de Tubino

; Lancman, Selma

; Tavares, Hermano

; Polanczyk, Guilherme

; Brunoni, André R.

; Forlenza, Orestes V.

; Barros-Filho, Tarcísio Eloy Pessoa de

Clinics

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2021, Volume 76 elocation e2631

Resumo: En | Texto: En | PDF: En

OBJECTIVE: In 2020, the COVID-19 pandemic brought a work and stress overload to healthcare workers, increasing their vulnerability to mental health impairments. In response, the authors created the COMVC-19 program. The program offered preventive actions and mental health treatment for the 22,000 workers of The Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP). This paper aims to describe its implementation and share what we have learned from this experience. METHODS: Workers were able to easily access the program through a 24/7 hotline. Additionally, a mobile phone app that screened for signs and symptoms of emotional distress and offered psychoeducation and/or referral to treatment was made available. Data from both these sources as well as any subsequent psychiatric evaluations were collected. RESULTS: The first 20 weeks of our project revealed that most participants were female, and part of the nursing staff working directly with COVID-19 patients. The most frequently reported symptoms were: anxiety, depression and sleep disturbances. The most common diagnoses were Adjustment, Anxiety, and Mood disorders. CONCLUSIONS: Implementing a mental health program in a multimodal intervention was feasible in a major quaternary public hospital. Our data also suggests that preventive actions should primarily be aimed at anxiety and depression symptoms, with a particular focus on the nursing staff.

https://doi.org/10.6061/clinics/2021/e2631

11.

COMPLEXOS DE PLATINA(II) CONJUGADO E ANÁLOGO A O-GLICOSÍDEOS: SÍNTESE, CARACTERIZAÇÃO ESTRUTURAL E ATIVIDADE ANTITUMORAL
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Lima, Lidiane M. A. de

; Santos, Mirelly D. F.

; Albuquerque, Leonardo S.

; Belian, Mônica F.

; Silva, Wagner E.

; R. F. Filho, João

; Santos, Jandyson M.

; Aguiar, Jaciana S.

; Silva, Teresinha G. da

Química Nova

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Jun 2020, Volume 43 Nº 6 Páginas 752 - 759

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

The synthesis, characterization, cytotoxicity and antitumor activity of platinum(II) complexes coordinated to O-glycoside and analogous is reported. Through the acute toxicity tests, it was possible to fit the complexes into categories according to the OECD protocol. The cis-[PtCl2(C6H14S2O2)-κ2S] complex was classified in category 3 and the cis-[PtCl2(C26H38O12S2)-κ2S] complex was classified in category 4. This result demonstrates the lower toxicity achieved by the glycoside in comparison with its analogous system. Evaluations of tumor masses extracted from mice inoculated with Ehrlich carcinoma demonstrated percent inhibition similar to cisplatin and synthesized platinum complexes. Cisplatin showed 59% inhibition, while cis-[PtCl2(C6H14S2O2)-κ2S] and cis-[PtCl2(C26H38O12S2)-κ2S] complexes presented 57% and 59%, respectively. Although the glycosidic compounds exhibit tumor activity, reported in the literature, the presence of platinum ion was shown to be determinant for antitumor action according with the compounds tested, since the glycosidic ligand presented tumor inhibition of only 38%, with a similar antitumor activity in relation to cisplatin.

https://doi.org/10.21577/0100-4042.20170546 390 downloads

12.

Crystal Structure and 1H NMR Experimental and Theoretical Study of Conformers of 5-Methyl-1-(4’-methylphenylsulfonylamino)-1H-[1,2,3]-triazole-4-carboxylic Acid Ethyl Ester and 5-Methyl-1-(phenylsulfonylamino)-1H-[1,2,3]-triazole-4-carboxylic Acid Ethyl Ester
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Freitas, Maria C. R.

; Campos, Vinicius R.

; Resende, Jackson A. L. C.

; Silva, Marcos M. P. da

; Ferreira, Vitor F.

; Cunha, Anna Claudia

; Carneiro, José W. M.

; Lage, Mateus R.

; Souza, Leonardo A. de

; Silva, Haroldo C.

; Almeida, Wagner B. de

Journal of the Brazilian Chemical Society

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Mai 2020, Volume 31 Nº 5 Páginas 867 - 885

Resumo: En | Texto: En | PDF: En

We reported experimental and theoretical investigation of conformers of 1,2,3-triazole derivatives, substances of exclusively synthetic origin, subject of extensive studies, because of several biological properties, such as antiviral, antimicrobial and antileishmaniasis. We reported molecular/supramolecular X-ray structures of antiophidian compounds I and II. For I and II there are two crystallographic different molecules in the unit cell (A and B). To explore the causes of the similarities in the compound’s crystal structures, intermolecular interactions were explored using the Hirshfeld surface as the fingerprint plots. In addition, density functional theory (DFT) calculations were carried out at the ωB97x-D/6-31G(d,p)-PCM-CHCl3 level aiming to contribute to the interpretation of the experimental data and complement the experimental findings. Two structures named 2A and 5B were found in good agreement with the respective X-ray solid state ones (A and B). Theoretical 1H nuclear magnetic resonance (NMR) spectra calculated for 5B rotated structure (torsion angles deviation around 40° to 90°) was in fine agreement with experimental results (in CDCl3) indicating that the solution molecular structure is considerably different from optimized equilibrium geometries and solid-state structure. Therefore, care is needed when using X-ray structures or DFT geometries to model interaction of drugs with biological targets since significant conformational changes may take place in solution.

https://doi.org/10.21577/0103-5053.20190249 267 downloads

13.

Thermal comfort monitoring in aviaries by a real-time data acquisition system
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Camargo, Tiago F. B.

; Silva, Robson L.

; Higa, Márcio

; Coutinho, Mônica R.

; Oliveira, Júlio C. D. de

; Conceição, Wagner A. dos S.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Set 2019, Volume 23 Nº 9 Páginas 694 - 701

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O conforto térmico no interior de instalações de criação de frangos de corte é essencial na obtenção de bons resultados nesta atividade de produção. A avaliação das condições termodinâmicas adequadas requer a medição e controle, geralmente implicando em custos e manutenção especializada. Neste trabalho, objetivou-se monitorar a distribuição de temperatura, umidade relativa e velocidade do ar utilizando sistema desenvolvido, de baixo custo, de código aberto e de fácil uso, com Arduíno (hardware) e Scilab (software) para aquisição de dados em tempo real. Os sensores foram instalados em uma unidade de produção existente (Cianorte, PR), com medições para o ambiente interno (20 sensores de temperatura/umidade e 2 sensores de pressão diferencial, respectivamente, a 0,5 e 1,5 m de altura) e para ambiente externo (pressão e velocidade do vento, a 1,5 m de altura). Sistema de aquisição de dados permitiu a comunicação com sensores, que são facilmente lidos por computador e armazenados em um arquivo de dados. O sistema de aquisição de dados desenvolvido provou-se eficiente quando aplicado em uma instalação de criação comercial de frangos de corte, permitindo o monitoramento em tempo real para os parâmetros de conforto térmico.

ABSTRACT Thermal comfort inside broiler husbandry facilities is essential in obtaining good results in the production activity. Assessment of adequate thermodynamic conditions requires measurement and control, usually implying costs and specialized maintenance. The objective of this research was to monitor the temperature, relative humidity and air speed distributions by a developed low-cost, open-source and easy-to-use measurement system, using Arduino (hardware) and Scilab (software) for real-time data acquisition. Sensors were installed in a real facility (Cianorte, PR), with measurements for internal ambient (20 sensors for temperature/relative humidity, and two sensors for differential pressure, respectively 0.5 and 1.5 m high) and external ambient (pressure and wind speed, 1.5 m high). Data acquisition system has enabled communication with sensors which are easily read by the computer and stored in a data file. The developed data acquisition system proved to be efficient when applied in a commercial broiler husbandry facility, enabling real-time monitoring for thermal comfort parameters.

https://doi.org/10.1590/1807-1929/agriambi.v23n9p694-701 1331 downloads

14.

Brazilian recommendations on the safety and effectiveness of the yellow fever vaccination in patients with chronic immune-mediated inflammatory diseases
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Pileggi, Gecilmara Salviato

; Mota, Licia Maria Henrique Da

; Kakehasi, Adriana Maria

; Souza, Alexandre Wagner De

; Rocha, Aline

; Melo, Ana Karla Guedes de

; Fonte, Caroline Araujo M. da

; Bortoletto, Cecilia

; Brenol, Claiton Viegas

; Marques, Claudia Diniz Lopes

; Zaltman, Cyrla

; Borba, Eduardo Ferreira

; Reis, Enio Ribeiro

; Freire, Eutilia Andrade Medeiros

; Klumb, Evandro Mendes

; Christopoulos, Georges Basile

; Laurindo, Ieda Maria M.

; Ballalai, Isabella

; Costa, Izaias Pereira Da

; Michelin, Lessandra

; Valadares, Lilian David de Azevêdo

; Chebli, Liliana Andrade

; Lacerda, Marcus

; Toscano, Maria Amazile Ferreira

; Yazbek, Michel Alexandre

; Vieira, Rejane Maria R. De Abreu

; Magalhães, Renata

; Kfouri, Renato

; Richtmann, Rosana

; Merenlender, Selma Da Costa Silva

; Valim, Valeria

; Assis, Marcos Renato De

; Kowalski, Sergio Candido

; Trevisani, Virginia Fernandes Moça

Advances in Rheumatology

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2019, Volume 59 elocation 17

Resumo: En | Texto: En | PDF: En

Abstract Background: In Brazil, we are facing an alarming epidemic scenario of Yellow fever (YF), which is reaching the most populous areas of the country in unvaccinated people. Vaccination is the only effective tool to prevent YF. In special situations, such as patients with chronic immune-mediated inflammatory diseases (CIMID), undergoing immunosuppressive therapy, as a higher risk of severe adverse events may occur, assessment of the risk-benefit ratio of the yellow fever vaccine (YFV) should be performed on an individual level. Main body of the abstract: Faced with the scarcity of specific orientation on YFV for this special group of patients, the Brazilian Rheumatology Society (BRS) endorsed a project aiming the development of individualized YFV recommendations for patients with CIMID, guided by questions addressed by both medical professionals and patients, followed an internationally validated methodology (GIN-McMaster Guideline Development). Firstly, a systematic review was carried out and an expert panel formed to take part of the decision process, comprising BRS clinical practitioners, as well as individuals from the Brazilian Dermatology Society (BDS), Brazilian Inflammatory Bowel Diseases Study Group (GEDIIB), and specialists on infectious diseases and vaccination (from Tropical Medicine, Infectious Diseases and Immunizations National Societies); in addition, two representatives of patient groups were included as members of the panel. When the quality of the evidence was low or there was a lack of evidence to determine the recommendations, the decisions were based on the expert opinion panel and a Delphi approach was performed. A recommendation was accepted upon achieving ≥80% agreement among the panel, including the patient representatives. As a result, eight recommendations were developed regarding the safety of YFV in patients with CIMID, considering the immunosuppression degree conferred by the treatment used. It was not possible to establish recommendations on the effectiveness of YFV in these patients as there is no consistent evidence to support these recommendations. Conclusion: This paper approaches a real need, assessed by clinicians and patient care groups, to address specific questions on the management of YFV in patients with CIMID living or traveling to YF endemic areas, involving specialists from many areas together with patients, and might have global applicability, contributing to and supporting vaccination practices. We recommended a shared decision-making approach on taking or not the YFV.

https://doi.org/10.1186/s42358-019-0056-x 1111 downloads

15.

Effect of Inoculation of Pineapple Plantlets with Arbuscular Mycorrhizal Fungi Obtained from Different Inoculum Sources Multiplied by the On-Farm Method
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Moreira, Bruno Coutinho

; Prates Junior, Paulo

; Jordão, Thuany Cerqueira

; Silva, Marliane de Cássia Soares da

; Ribeiro, Ana Paula Ferreira

; Stürmer, Sidney Luiz

; Salomão, Luiz Carlos Chamhum

; Otoni, Wagner Campos

; Kasuya, Maria Catarina Megumi

Revista Brasileira de Ciência do Solo

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2019, Volume 43 elocation e0180148

Resumo: En | Texto: En | PDF: En

ABSTRACT The production of inoculum from arbuscular mycorrhizal fungi (AMF) at a large scale and low cost is essential for establishing methods to assist in producing pineapple plantlets with high nutritional and phytosanitary quality. However, this objective is difficult to accomplish because of the biotrophic nature of these fungi. The on-farm multiplication method for AMF inoculum presents a good alternative to supply the demand for the production of glomerospores. This study aimed to multiply and evaluate AMF inoculum originating from isolated species (including Rhizophagus clarus, Claroideoglomus etunicatum) versus native AMF from pineapple and coffee plantations multiplied by the on-farm method on the colonization in pineapple plantlets. Initially, inocula of R. clarus, C. etunicatum, and native AMF (pineapple and coffee) were multiplied by the on-farm method in Sorghum bicolor. After four months, the number of AMF spores and the percentage of viable spores at the layers of 0.00-0.05 and 0.05-0.10 m were evaluated. There were no differences in spore numbers in relation to the source of the inoculum (R. clarus, C. etunicatum, pineapple, and coffee) and evaluated layers, with an average number of 605 spores per 100 cm3 of soil. The percentage of viable spores was greater at the layer of 0.00-0.05 m (76.32 %) compared to the layer of 0.05-0.10 m (72.05 %), regardless of the inoculum source. The viability of the inoculum obtained from C. etunicatum was higher than that from the coffee crop (77.93 and 68.06 %, respectively). Subsequently, the spores were inoculated in pineapple plantlets to assess the rate of colonization. Pineapple plantlets inoculated with AMF had an average of colonization of 18 and 67.73 % after 50 and 180 days cultivation, respectively, with no significant difference being detected between treatments. Therefore, on-farm inoculum production was effective at multiplying the AMF of both isolates of R. clarus and C. etunicatum, as well as for commercial crops (pineapple and coffee), with spores having high viability. Arbuscular mycorrhizal fungi colonized pineapple plantlets independently of the inoculum utilized and favored its growth.

https://doi.org/10.1590/18069657rbcs20180148 1570 downloads

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