Resumo Foi investigada a ocorrência de parasitos gastrintestinais com potencial zoonótico, em cães de área rural do munícipio de Painel, Santa Catarina, Brasil. Para isso, foram colhidas amostras de fezes de 91 cães de 82 propriedades rurais, no período de agosto de 2017 a janeiro de 2018. Este material fecal foi processado pelas técnicas de sedimentação espontânea, centrífugo-flutuação em sulfato de zinco e coloração pelo Ziehl-Neelsen. Um questionário em forma de entrevista foi aplicado aos tutores dos cães e foram obtidas informações sobre a propriedade rural e principais cuidados com os cães. Entre as 91 amostras de fezes, em 27 (29,7%) foi detectada a presença de parasitos gastrintestinais, sendo Ancylostoma o gênero mais prevalente (15 / 16,5%), seguido por Giardia duodenalis (13 / 14,3%), Trichuris vulpis (6 / 6,6%), Toxocara canis (5 / 5,5%), Entamoeba spp. (4 / 4,8%), Cryptosporidium spp. (3 / 3,3%) e Taeniidae (1 / 1,1%). A maioria dos cães não recebia cuidados veterinários ou antiparasitários. Concluiu-se que os cães de área rural albergam parasitos gastrintestinais, porém seus tutores desconhecem os riscos que esses parasitos podem trazer para a saúde humana. zoonótico Painel Catarina Brasil isso 9 8 rurais 201 2018 espontânea centrífugoflutuação centrífugo flutuação ZiehlNeelsen. ZiehlNeelsen Ziehl Neelsen. Neelsen Ziehl-Neelsen 2 29,7% 297 29 7 (29,7% 15 16,5%, 165 16,5% , 16 5 16,5%) 13 14,3%, 143 14,3% 14 3 14,3%) 6 ( 6,6%, 66 6,6% 6,6%) 5,5%, 55 5,5% 5,5%) spp 4 4,8%, 48 4,8% 4,8%) 3,3% 33 1 1,1%. 11 1,1% . 1,1%) antiparasitários Concluiuse Concluiu se humana 20 29,7 (29,7 16,5 14,3 6,6 5,5 4,8 3,3 1,1 29, (29, 16, 14, 6, 5, 4, 3, 1, (29 (2

Abstract We investigated the occurrence of gastrointestinal parasites in dogs in rural areas of the municipality of Painel, Santa Catarina, Brazil. For this, 91 canine feces samples were collected from 82 farms between August, 2017 and January, 2018. These fecal materials were processed using the techniques of spontaneous sedimentation, centrifugation-floatation in zinc sulfate and Ziehl-Neelsen staining. A questionnaire in the form of an interview was administered to the dogs’ owner and information about the farm and the main care provided for the dogs was obtained. Among 91 sampled dogs, 27 (29.7%) were positive for at least one parasite species. Ancylostoma was the most prevalent genus (16.5%), followed by Giardia duodenalis (14.3%), Trichuris vulpis (6.6%), Toxocara canis (5.5%), Entamoeba spp. (4.8%), Cryptosporidium spp. (3.3%) and Taeniidae (1.1%). Most dogs do not receive veterinarian care and rarely received antiparasitic treatment. They were free to roam and had free access to animal remains and garbage, which was reflected in the significant associations with the occurrence of parasites that were found. We conclude that rural dogs harbor gastrointestinal parasites, but that their owners are unaware of the risks that these parasites can bring to human health. Painel Catarina Brazil this 9 8 August 201 January 2018 sedimentation centrifugationfloatation centrifugation floatation ZiehlNeelsen Ziehl Neelsen staining obtained 2 29.7% 297 29 7 (29.7% species 16.5%, 165 16.5% , 16 5 (16.5%) 14.3%, 143 14.3% 14 3 (14.3%) 6.6%, 66 6.6% 6 (6.6%) 5.5%, 55 5.5% (5.5%) spp 4.8%, 48 4.8% 4 (4.8%) 3.3% 33 (3.3% 1.1%. 11 1.1% . 1 (1.1%) treatment garbage found health 20 29.7 (29.7 16.5 (16.5% 14.3 (14.3% 6.6 (6.6% 5.5 (5.5% 4.8 (4.8% 3.3 (3.3 1.1 (1.1% 29. (29. 16. (16.5 14. (14.3 6. (6.6 5. (5.5 4. (4.8 3. (3. 1. (1.1 (29 (16. (14. (6. (5. (4. (3 (1. (2 (16 (14 (6 (5 (4 ( (1

Abstract: Purpose: to evaluate the effect of dentifrice pH and fluoride concentration ([F]) on fluoride uptake on the biofilm and nails of children from a non-fluoridated area. Methods: two hundred and twenty-eight two- to four-year-old children were randomly allocated into 3 groups according to the type of dentifrice: G1: 1100 μg F/g, pH 4.5 (n = 76); G2: 750 μg F/g, pH 4.5 (n = 74); and G3: 1100 μg F/g, pH 7.0 (n = 78). Nails were collected at 4, 8, and 12 months after starting dentifrice use and biofilm was collected 5 and 60 minutes after toothbrushing. The concentrations of F in nails and biofilm were analyzed by HMDS facilitated diffusion. Data were analyzed by Kruskal-Wallis/Mann-Whitney's test and the comparison between biofilm collection times was done using Wilcoxon test (p £ 0.05). Results: a significant reduction of [F] in biofilm was observed 60 minutes after toothbrushing, regardless of the dentifrice used. However, 5 minutes after toothbrushing, G1 had a significantly higher [F] compared to G2 and G3, and 60 minutes after toothbrushing, [F] was significantly higher for G1 and G2 compared to G3. G1 and G3 had significantly higher [F] in the nails compared to G2. Conclusion: a lower dentifrice concentration is a relevant factor for the reduction of excessive fluoride intake. The use of a low-F acidified dentifrice combines the reduction of fluoride uptake with caries prevention by leading to greater incorporation of F into the biofilm over time.

Herein, we report the concentration of ethyl carbamate (EC) and copper in 380 samples of sugar-cane spirit and 45 samples of manioc spirit as determined by GC-MS and FAAS respectively. The cyanide content determined spectrophotometrically is reported for the manioc spirit. Sugar cane spirit produced by alembic distillation (70,0 µg L-1) shown a lower content of EC than samples produced by column distillation (270 µg L-1). No simple correlation between the content of EC and copper for sugar cane spirit as well among the concentration of EC, copper, and cyanide for manioc spirit could be observed.