ABSTRACT Introduction Chemotherapy and radiation therapy are considered standard treatments for stage II seminoma patients; however, these therapies are associated with long-term toxicities. Recently, retroperitoneal lymph node dissection has emerged as an alternative strategy, and the first three phase II trials were published in 2023 with promising results. The present study conducted a systematic review and meta-analysis to evaluate this surgery as an alternative treatment for stage IIA/B seminoma patients. Purpose Seminomas are the most common testicular tumors, often affecting young adult males. Standard treatments for stage II seminomas include chemotherapy and radiation therapy, but these therapies are associated with long-term toxicities. Thus, identifying alternative strategies is paramount. Herein, we conducted a systematic review and meta-analysis to appraise the efficacy and safety of retroperitoneal lymph node dissection (RPLND) for treating this condition. Methods We systematically searched the PubMed, Embase, and Cochrane databases for studies evaluating RPLND as a primary treatment for stage II A/B seminomas. Using a random-effects model, single proportion and means and pooled 2-year recurrence-free survival rates with hazard rates and 95% CI were calculated. Results Seven studies were included, comprising 331 males with stage II seminomas. In the pooled analysis, the recurrence rate was 17.69% (95% CI 12.31–24.75), and the 2-year RFS rate was 81% (95% CI 0.77–0.86). The complication rate was 9.16% (95% CI 6.16–13.42), the Clavien–Dindo > 2 complication rate was 8.83% (95% CI 5.76–13.31), and the retrograde ejaculation rate was 7.01% (95% CI 3.54–13.40). The median operative time was 174.68 min (95% CI 122.17–249.76 min), median blood loss was 105.91 mL (95% CI 46.89–239.22 mL), and patients with no evidence of lymph node involvement ranged from 0–16%. Conclusions Primary RPLNDs for treating stage IIA/B seminomas have favorable RFS rates, with low complication and recurrence rates. These findings provide evidence that this surgery is a viable alternative therapy for these patients. however longterm long term toxicities Recently strategy 202 results metaanalysis meta analysis IIAB IIA B tumors Thus paramount Herein (RPLND condition PubMed Embase AB A randomeffects random effects model 2year year recurrencefree free 95 calculated included 33 1769 17 69 17.69 (95 12.31–24.75, 12312475 12.31–24.75 , 12 31 24 75 12.31–24.75) 81 0.77–0.86. 077086 0.77–0.86 . 0 77 86 0.77–0.86) 916 9 16 9.16 6.16–13.42, 6161342 6.16–13.42 6 13 42 6.16–13.42) ClavienDindo Clavien Dindo 883 8 83 8.83 5.76–13.31, 5761331 5.76–13.31 5 76 5.76–13.31) 701 7 01 7.01 3.54–13.40. 3541340 3.54–13.40 3 54 40 3.54–13.40) 17468 174 68 174.6 1221724976 122 249 122.17–249.7 min, min) 10591 105 91 105.9 468923922 46 89 239 22 46.89–239.2 mL, mL) 016 0–16% 20 176 1 17.6 (9 1231247 12.31–24.7 07708 0.77–0.8 9.1 616134 6.16–13.4 4 88 8.8 576133 5.76–13.3 70 7.0 354134 3.54–13.4 1746 174. 122172497 122.17–249. 1059 10 105. 46892392 23 46.89–239. 0–16 17. ( 123124 12.31–24. 0770 0.77–0. 9. 61613 6.16–13. 8. 57613 5.76–13. 7. 35413 3.54–13. 12217249 122.17–249 4689239 46.89–239 0–1 12312 12.31–24 077 0.77–0 6161 6.16–13 5761 5.76–13 3541 3.54–13 1221724 122.17–24 468923 46.89–23 0– 1231 12.31–2 07 0.77– 616 6.16–1 576 5.76–1 354 3.54–1 122172 122.17–2 46892 46.89–2 123 12.31– 0.77 61 6.16– 57 5.76– 35 3.54– 12217 122.17– 4689 46.89– 12.31 0.7 6.16 5.76 3.54 1221 122.17 468 46.89 12.3 0. 6.1 5.7 3.5 122.1 46.8 12. 6. 5. 3. 122. 46.

ABSTRACT Background: Helicobacter pylori (H. pylori) is a gram-negative bacterium associated with the etiology of several gastrointestinal tract pathologies, and cagA-positive (cagA+) strains are found in populations with gastric ulcers and precancerous lesions, inducing pro-inflammatory responses. The development of neoplasms is related to microRNA (miRNA) dysregulation, indicating highly expressed miRNA-629. The article aims to correlate the expression level of miRNA-629 with the presence of H. pylori and the pathogenicity marker cagA. Methods: 203 gastric biopsy samples were evaluated from individuals with normal gastric tissue (n=60), gastritis (n=96), and gastric cancer (n=47) of both genders and over 18 years old. The samples were subdivided according to the presence or absence of H. pylori, detected by polymerase chain reaction (PCR). RNA was extracted using a commercial kit and quantified. Complementary DNA (cDNA) was synthesized using commercial kits, and the relative expression was calculated using the 2-ΔΔCt method. Results: Individuals infected with H. pylori are nine times more likely to develop gastric cancer. Cancer patients appeared to have decreased expression of miRNA-629; however, the presence of the bacterium would not influence this reduction. Individuals in the cancer group showed lower miRNA-629 expression when cagA+; however, in the control group, the expression was higher when cagA+. Conclusion: H. pylori is a factor involved in the etiology and progression of gastric diseases. Reduction in miRNA-629 expression in cancer patients occurs independent of the presence of the bacterium, but when the cagA pathogenicity marker is present, it induces changes in the gene expression of the respective miRNA. Background H (H gramnegative gram negative pathologies cagApositive positive cagA+ (cagA+ lesions proinflammatory pro inflammatory responses miRNA (miRNA dysregulation miRNA629. miRNA629 629. 629 miRNA-62 Methods 20 n=60, n60 n n=60 , 60 (n=60) n=96, n96 n=96 96 (n=96) n=47 n47 47 (n=47 1 old PCR. PCR . (PCR) quantified cDNA (cDNA kits 2ΔΔCt ΔΔCt 2 method Results however reduction Conclusion diseases present (cagA miRNA62 62 miRNA-6 n6 n=6 6 (n=60 n9 n=9 9 (n=96 n=4 n4 4 (n=4 (PCR miRNA6 miRNA- n= (n=6 (n=9 (n= (n

RESUMO Contexto: Helicobacter pylori (H. pylori) é uma bactéria gram-negativa associada à etiologia de várias patologias do trato gastrointestinal, e cepas positivas para cagA (cagA+) são encontradas em populações com úlceras gástricas e lesões pré-cancerígenas, induzindo respostas pró-inflamatórias. O desenvolvimento de neoplasias está relacionado à desregulação do microRNA (miRNA), indicando miRNA-629 altamente expresso. O artigo tem como objetivo correlacionar o nível de expressão do miRNA-629 com a presença de H. pylori e o marcador de patogenicidade cagA. Métodos: Foram avaliadas 203 amostras de biópsia gástrica de indivíduos com tecido gástrico normal (n=60), gastrite (n=96) e câncer gástrico (n=47) de ambos os sexos e com mais de 18 anos. As amostras foram subdivididas de acordo com a presença ou ausência de H. pylori, detectado por reação em cadeia da polimerase (PCR). O RNA foi extraído usando um kit comercial e quantificado. O DNA complementar (cDNA) foi sintetizado usando kits comerciais, e a expressão relativa foi calculada usando o método 2-ΔΔCt. Resultados: Indivíduos infectados com H. pylori têm nove vezes mais chances de desenvolver câncer gástrico. Pacientes com câncer parecem ter diminuição da expressão do miRNA-629; no entanto, a presença da bactéria não influenciaria essa redução. Indivíduos no grupo do câncer apresentaram menor expressão do miRNA-629 quando cagA+; no entanto, no grupo controle, a expressão foi maior quando cagA+. Conclusão: H. pylori é um fator envolvido na etiologia e progressão das doenças gástricas. A redução na expressão do miRNA-629 em pacientes com câncer ocorre independentemente da presença da bactéria, mas quando o marcador de patogenicidade cagA está presente, induz mudanças na expressão gênica do respectivo miRNA. Contexto H (H gramnegativa gram negativa gastrointestinal cagA+ (cagA+ précancerígenas, précancerígenas pré cancerígenas, cancerígenas pré-cancerígenas próinflamatórias. próinflamatórias pró inflamatórias. inflamatórias pró-inflamatórias miRNA, miRNA , (miRNA) miRNA629 629 miRNA-62 expresso Métodos 20 n=60, n60 n n=60 60 (n=60) n=96 n96 96 (n=96 n=47 n47 47 (n=47 1 anos PCR. PCR . (PCR) quantificado cDNA (cDNA comerciais 2ΔΔCt. 2ΔΔCt ΔΔCt 2 ΔΔCt. 2-ΔΔCt Resultados entanto controle Conclusão presente (cagA (miRNA miRNA62 62 miRNA-6 n6 n=6 6 (n=60 n=9 n9 9 (n=9 n=4 n4 4 (n=4 (PCR miRNA6 miRNA- n= (n=6 (n= (n

ABSTRACT Objective: To analyze the molecular composition of a sample of Baltic amber taken from an amber bracelet, verify the presence or absence of compounds beneficial to human health, and discuss the therapeutic potential of this fossil resin. Material and Methods: For this, a qualitative analysis was performed by gas chromatography coupled with mass spectrometry (GC-MS system), which can perform an automated separation into individual components of amber. Results: The samples comprised terpenes and terpenoids: monoterpenoids, sesquiterpenoids, diterpenoids, hydronaphthalene, succinic acid, and isopimaric acid. These have therapeutic potential for several diseases, inhibit several stages of the inflammatory process, and improve the symptoms caused by it. Conclusion: The amber of this sample tested is formed by terpenes and terpenoids, substances with anti-inflammatory and analgesic. However, an in-depth study is needed on the release and absorption of substances in human skin or their actual effectiveness. Objective bracelet health resin Methods GCMS GC MS system, system , system) Results terpenoids monoterpenoids sesquiterpenoids diterpenoids hydronaphthalene acid diseases process it Conclusion antiinflammatory anti analgesic However indepth depth effectiveness

ABSTRACT Agricultural productivity is closely related to soil physical attributes, specifically those that affect the soil-water relationship, as the soil serves as the main water reservoir for plants. This research aimed to determine the field capacity for different soils, using equations based on the water retention curve. The database used included 150 soil profiles from studies published by other authors encompassing information related to textural classification, soil bulk density, particle density and soil water retention. The inflection point for each soil profile and the corresponding matrix potential were generated. Multiple correlations were established between volumetric moisture at field capacity and clay, silt and sand contents. The calculated inflection point can be an estimator of field capacity, what may facilitate and speed up the calculation of water availability. attributes soilwater relationship plants soils curve 15 classification generated clay contents availability 1

RESUMO A produtividade agrícola está intimamente relacionada aos atributos físicos do solo, em especial àqueles que afetam a relação solo-água, uma vez que o solo se constitui no principal reservatório hídrico para as plantas. Objetivou-se determinar a capacidade de campo para diferentes solos, a partir de equações baseadas na curva de retenção de umidade. Foi utilizado um banco de dados com 150 perfis obtidos de trabalhos publicados por outros autores, com informações sobre classificação textural, densidade aparente do solo, densidade de partícula e retenção de água no solo. Foi gerado, para cada perfil, o ponto de inflexão, assim como o potencial matricial correspondente ao seu valor. Foram estabelecidas correlações múltiplas entre a umidade volumétrica na capacidade de campo e os teores de argila, silte e areia. O ponto de inflexão calculado pode ser um estimador da capacidade de campo, o que pode facilitar e agilizar o cálculo de disponibilidade de água. soloágua, soloágua água, solo-água plantas Objetivouse Objetivou solos 15 autores textural gerado perfil valor argila areia 1

ABSTRACT Peach palm (Bactris gasipaes) is a native tree from Brazil widely used for obtaining palm heart. Due to the increasing interest in palm heart, plantations are expanding to the Brazilian South and Southeast regions. Peach palm processing generates high amounts of solid wastes, which are inadequately disposed of and have no consolidated use. Proposing potential utilizations for the valorization of these lignocellulosic matrices requires knowing their chemical composition. This work aimed to determine the chemical composition (at mineral, nutritional, and macromolecular levels) of the three by-products (internal sheath, external sheath, and basal portion) generated during the processing of the peach palm, to characterize their semi-volatile compounds, and to compare them to the edible palm heart. The by-products represent 83.6% (w/w) of the biomass in the peach palm processing and have a high dietary fiber content (59.2 - 68.1%). Internal sheath and basal portion showed high protein content (8.40 - 11.8%) according to Brazilian legislation and several bioactive compounds such as myo-inositol and organic acids (succinic, gallic, and linoleic acids), indicating their nutraceutical properties and potential to be used as food additives or ingredients in food formulation. Besides, the external sheath had high cellulose content (39.6%) that could be extracted and applied in material science. All by-products have compounds of interest to the industry and a high potential to be employed in the development of products with higher added value. Bactris gasipaes heart regions wastes use at mineral nutritional levels byproducts by internal semivolatile semi volatile 836 83 6 83.6 w/w ww w (w/w 59.2 592 59 2 (59. 68.1%. 681 68.1% . 68 1 68.1%) 8.40 840 8 40 (8.4 11.8% 118 11 myoinositol myo inositol succinic, succinic (succinic gallic acids, , acids) formulation Besides 39.6% 396 39 (39.6% science value 83. 59. 5 (59 68.1 8.4 84 4 (8. 11.8 39.6 3 (39.6 (5 68. 8. (8 11. 39. (39. ( (39 (3

RESUMO A pupunha (Bactris gasipaes) é uma planta nativa do Brasil amplamente utilizada na obtenção de palmito nas regiões Sul e Sudeste do Brasil. Seu processamento gera altas quantidades de resíduos sólidos, que são descartados inadequadamente e não têm uso consolidado. Propor potenciais utilizações para a valorização destas matrizes lignocelulósicas requer conhecer a composição química de tais materiais, que não foi amplamente apresentada na literatura. Os objetivos do presente trabalho foram determinar a composição química dos três subprodutos gerados no processamento do palmito de pupunha com relação a suas composições mineral, nutricional e macromolecular, caracterizar os compostos semivoláteis de tais materiais, e compará-los ao palmito, a parte comestível da pupunha. Os subprodutos representam 83.6% (m/m) da biomassa processada na agroindústria de pupunha e apresentam um alto teor de fibras alimentares (59.2 - 68.1%). A Bainha Interna e a Parte Basal apresentaram alto teor de proteína (8.40 - 11.8%) de acordo com a legislação brasileira e vários compostos bioativos como mio-inositol e ácidos succínico, gálico e linoleico, indicando suas propriedades nutracêuticas e potencial para uso como aditivos ou ingredientes na formulação de alimentos devido a sua composição nutricional. Ainda, a Bainha Externa apresentou alto teor de celulose (39.6%), que poderia ser extraída e aplicada na área de ciência dos materiais. Todos os subprodutos têm um teor considerável de compostos de interesse para a indústria e um alto potencial para emprego no desenvolvimento de produtos com alto valor agregado. Bactris gasipaes sólidos consolidado materiais literatura mineral macromolecular comparálos compará los 836 83 6 83.6 m/m mm m (m/m 59.2 592 59 2 (59. 68.1%. 681 68.1% . 68 1 68.1%) 8.40 840 8 40 (8.4 11.8% 118 11 mioinositol mio inositol succínico linoleico Ainda 39.6%, 396 39.6% , 39 (39.6%) agregado 83. 59. 5 (59 68.1 8.4 84 4 (8. 11.8 39.6 3 (39.6% (5 68. 8. (8 11. 39. (39.6 ( (39. (39 (3

Abstract: Aim To explore the short-term effects of rainfall events on the biomass, density, and richness of the phytoplankton community during dry and rainy periods, as well as on the selection and response of Morphology-Based Functional Groups (MBFG). Methods The phytoplankton community and abiotic environmental variables were sampled over a short period in a subtropical urban lake during the dry and rainy seasons (2018-2019). Generalized Linear Models (GLMs) were generated to analyze the relationship between phytoplankton biovolume, density, and richness with abiotic variables. The predictability of phytoplankton functional groups was assessed using Redundancy Analysis (RDA). Results There was an increase in the density and biovolume of the phytoplankton community during the rainy period. Species richness decreased with increased rainfall. The lake exhibited a high dominance of Cyanobacteria (MBFG VIII), mainly represented by Raphidiopsis raciborskii (Woloszynska) Aguilera in both periods studied. Conclusions We found evidence supporting the hypothesis that rainfall events increase the density and biovolume of phytoplankton. Morphology-based functional groups served as efficient indicators of the lake's environmental conditions. Abstract shortterm term biomass MorphologyBased Morphology Based MBFG. MBFG . (MBFG) 20182019. 20182019 2018 2019 (2018-2019) GLMs (GLMs RDA. RDA (RDA) VIII, VIII , VIII) Woloszynska (Woloszynska studied Morphologybased based lakes s conditions 2018201 201 (2018-2019 (RDA 201820 20 (2018-201 20182 2 (2018-20 (2018-2 (2018- (2018 (201 (20 (2 (

Resumo: Objetivo Explorar os efeitos a curto prazo de eventos de chuva na biomassa, densidade e riqueza da comunidade fitoplanctônica durante os períodos seco e chuvoso, bem como na seleção e resposta de Grupos Funcionais Baseados na Morfologia (MBFG). Métodos A comunidade fitoplanctônica e as variáveis ambientais abióticas foram amostradas em um curto período em um lago urbano subtropical durante as estações seca e chuvosa (2018-2019). Modelos Lineares Generalizados (GLMs) foram gerados para analisar a relação entre o biovolume, a densidade e a riqueza do fitoplâncton com as variáveis abióticas. A previsibilidade dos grupos funcionais de fitoplâncton foi avaliada usando Análise de Redundância (RDA). Resultados Houve um aumento da densidade e do biovolume da comunidade fitoplanctônica no período chuvoso. A riqueza de espécies diminuiu com o aumento da pluviosidade. O lago exibiu uma alta dominância de Cyanobacteria (MBFG VIII), principalmente representadas por Raphidiopsis raciborskii (Woloszynska) Aguilera nos dois períodos estudados. Conclusões Encontramos evidências que sustentam a hipótese de que eventos de chuva aumentam a densidade e o biovolume do fitoplâncton. Os grupos funcionais baseados na morfologia serviram como indicadores eficientes das condições ambientais do lago. Resumo biomassa chuvoso MBFG. MBFG . (MBFG) 20182019. 20182019 2018 2019 (2018-2019) GLMs (GLMs RDA. RDA (RDA) pluviosidade VIII, VIII , VIII) Woloszynska (Woloszynska estudados 2018201 201 (2018-2019 (RDA 201820 20 (2018-201 20182 2 (2018-20 (2018-2 (2018- (2018 (201 (20 (2 (

Abstract This article aims to present a model of how the decision-making process can drive the development of dynamic capabilities for generating sustainable innovation in cleantech companies. Cleantechs are highly technological organizations, one of whose main characteristics is little or no use of non-renewable resources to produce any type of product, service, or process. The use of these technologies and identification of opportunities in the market makes sustainable innovation the core competence of this type of company. A multiple case study was conducted with cleantechs using a qualitative approach, supported by the content analysis technique. The primary data collection was carried out via semi-structured interviews, between October of 2020 and December of 2021, using an interview script as a collection instrument. The results provide a model that illustrates how the decision-making process can drive the development of dynamic capabilities for generating sustainable innovations, by means of categories that reflect technological sustainability, spillover effects, and a drive toward digitalization in clean technologies. The scientific contribution of the study is the presentation of how organizations can restructure their business models and develop sustainable and technological innovations, digitally leapfrogging to address bottlenecks related to energy rationing, social inclusion through monitoring technologies, access to low-cost clean and renewable energy, and other solutions. decisionmaking decision making companies nonrenewable non product service company approach technique semistructured semi structured interviews 202 2021 instrument innovations sustainability effects rationing lowcost low cost solutions 20 2

Resumo O objetivo do artigo é apresentar um modelo de como o processo decisório pode impulsionar o desenvolvimento de capacidades dinâmicas para geração de inovação sustentável das empresas cleantech . As cleantechs são organizações altamente tecnológicas que têm como uma de suas principais características a baixa ou nenhuma utilização de recursos não renováveis para produzir qualquer tipo de produto, serviço ou processo. A utilização dessas tecnologias e identificação de oportunidades no mercado torna a inovação sustentável a core competence desse tipo de empresa. Realizou-se um estudo de caso múltiplo de abordagem qualitativa com cleantechs , apoiado pela técnica de análise de conteúdo. A coleta dos dados primários foi feita por meio de entrevistas semiestruturadas, entre outubro de 2020 e dezembro de 2021, utilizando um roteiro de entrevista como instrumento de coleta. Os resultados fornecem um modelo que ilustra como o processo decisório pode impulsionar o desenvolvimento de capacidades dinâmicas para a geração de inovações sustentáveis, por meio de categorias que refletem a sustentabilidade tecnológica, efeitos de transbordamento e impulsionamento para digitalização em tecnologias limpas. A contribuição científica do trabalho é a apresentação de como as organizações podem reestruturar seus modelos de negócios e desenvolver inovações sustentáveis e tecnológicas, proporcionando um salto digital para solucionar gargalos relacionados a racionamento energético, inclusão social por meio de tecnologias de monitoramento, acesso à energia limpa e renovável a baixo custo e outras soluções. produto empresa Realizouse Realizou se conteúdo semiestruturadas 202 2021 tecnológica limpas energético monitoramento soluções 20 2

Abstract In this work, measuring capabilities of a Sagnac Interferometer-based Distributed Optical Fiber Sensor (DOFS) system are demonstrated by comparing its results to a conventional amplitude-based Distributed Acoustic Sensing (DAS) system. Through a set of three different experiments, it was demonstrated that both sensing systems can detect and locate dynamical deformation along the fiber, in addition to determining its frequency components. The coherent amplitude-based DAS presented a lower background noise. In contrast, even though the Sagnac interferometry-based system presented low-frequency noise in the measurements, it is less susceptible to harmonic distortion for higher amplitude deformations since its sensing principles rely on the measurement of the accumulated phase rather than the amplitude of the backscattered signal purely. Accordingly, the Sagnac interferometry-based system arises as a promising and rather accessible topology for a DOFS implementation. work Interferometerbased Interferometer based (DOFS amplitudebased (DAS experiments fiber components contrast interferometrybased interferometry lowfrequency low measurements purely Accordingly implementation

Abstract Behavioral characteristics may also take part in the etiology of dental caries. Sedentary behavior, especially watching television, is associated with increased intake of foods high in fat or free sugar, which could influence the occurrence of dental caries. The aim of this study was to assess the mediating effect of eating pattern on the relationship between television exposure time and the presence of dental caries in children. A cross-sectional study was conducted with a representative sample of 580 parent-child dyads with children aged 7 to 8 years in 20 public schools in Pelotas, Brazil. Parents or legal guardians were interviewed and provided information on demographic/socioeconomic data, children’s daily television exposure time, and answered the Biological Rhythms Interview for Assessment in Neuropsychiatry for Kids (BRIAN-K-eating pattern domain). Caries was evaluated through clinical examination. The mediating effect of eating pattern on the relationship between television exposure and presence of dental caries was estimated using the parametric g-formula. Prevalence of dental caries was 63%, and 22% of children watched TV 4 or more hours per day. Television exposure had no direct effect on the dental caries experience [odds ratio (OR): 1.05 (95% confidence interval (95%CI): 0.92–1.19)]. Nevertheless, difficulty maintaining regular eating pattern mediated the natural indirect effect of television exposure time (≥ 4 hours/day) on dental caries experience [OR: 1.07 (95%CI): 1.01–1.14)]. The results of this study confirm the indirect pathway between television viewing and dental caries and the role of disordered eating patterns in this association. behavior sugar crosssectional cross sectional 58 parentchild parent child 2 Pelotas Brazil demographicsocioeconomic demographic socioeconomic data childrens s BRIANKeating BRIAN K domain. domain . domain) examination gformula. gformula g formula. formula g-formula 63 63% 22 day odds OR (OR) 105 1 05 1.0 95% 95 (95 95%CI 95CI CI (95%CI) 0.92–1.19. 092119 0.92–1.19 0 92 19 0.92–1.19)] Nevertheless ≥ ( hours/day hoursday [OR 107 07 1.01–1.14. 101114 1.01–1.14 01 14 1.01–1.14)] association 5 6 (OR 10 1. 9 (9 (95%CI 09211 0.92–1.1 0.92–1.19) 10111 1.01–1.1 1.01–1.14) 0921 0.92–1. 1011 1.01–1. 092 0.92–1 101 1.01–1 09 0.92– 1.01– 0.92 1.01 0.9 0.

Abstract Human reproduction goes through many challenges to its success and in many cases it fails. Cases of pregnancy loss are common outcomes for pregnancies, and implantation failures (IF) are common in assisted reproduction attempts. Although several risk factors have already been linked to adverse outcomes in reproduction, many cases remain without a definitive cause. Genetics of female reproduction is a field that may bring some pieces of this puzzle; however, there are no well-defined genes that might be related to the risk for recurrent pregnancy loss (RPL) and IF. Here, we present a literature review of the studies of genetic association in RPL and IF carried out in the Brazilian population and complemented with a database search to explore genes previously related to RPL and IF, where a search for genes previously involved in these conditions was performed in OMIM, HuGE, and CTD databases. Finally, we present the next steps for reproductive genetics investigation, through genomic sequencing analyses and discuss future plans in the study of RPL genetics. The combined strategy of looking for literature and databases is useful to raise hypotheses and to identify underexplored genes related to RPL and IF. fails pregnancies (IF attempts cause puzzle however welldefined well defined (RPL Here OMIM HuGE Finally investigation

ABSTRACT Brazilian spotted fever, a zoonotic disease transmitted by ticks, is caused by Rickettsia rickettsii. We report a fulminant case of this zoonosis in a healthy 46-year-old military man in the urban region of Rio de Janeiro city, in October, 2021. Ticks and capybaras (Amblyomma sculptum, Hydrochoerus hydrochaeris, respectively) were identified in the military fields, pointing to the participation of this large synanthropic rodent, recognized as an efficient amplifier host of Rickettsia rickettsii in Brazil. As the military population is considered a risk group for spotted fever, it is necessary to alert health professionals to the importance of the early detection of the disease and its adequate management, mainly in populations that are particularly at risk of exposure to ticks, in order to avoid fatal outcomes.

Resumo O objetivo deste trabalho foi quantificar os teores de C das frações químicas e físicas da MOS em diferentes sistemas de manejo em um Argissolo textura arenosa. O estudo foi realizado em uma área de referência de Mata Nativa (MN) e três áreas manejadas: pastagem permanente (PP), sistema plantio direto (SPD) e área de Reserva Particular de Patrimônio Natural em processo de regeneração natural (RPPN). Foram coletadas amostras de solos das camadas de 0-0,05, 0,05-0,10 e 0,10-0,20 m. Foi determinado a densidade do solo (Ds) os teores de carbono orgânico total (COT) o fracionamento químico da MOS com determinação dos teores de C dos ácidos fúlvicos (AF), ácidos húmicos (AH) e humina (HUM), com posteriores cálculos das relações AH/AF, EA/HUM, estoque (EstC), fracionamento físico-granulométrico e determinação dos teores de C da matéria orgânica particulada (C-MOP) e índice de manejo de carbono (IMC). Observou-se maiores teores de COT para a área de MN, especialmente nas camadas de 0-0,05 e 0,05-0,10 m. Os teores de C-HUM predominam em relação aos teores de C-AH e C-AF. Os teores de C-AH e de C-HUM foram superiores em MN e SPD. A MN apresentou maiores teores de C-MOP em todas as camadas avaliadas. Para o C-MOM a área de SPD foi superior as demais áreas manejadas. As áreas manejadas apresentaram valores de EstMOP inferiores à MN. Nas duas primeiras camadas, a área de MN apresentou maiores EstMOM. As áreas manejadas apresentaram valores inferiores de IMC em relação a MN. A área de SPD demonstrou que, mesmo em sucessão, contribui para melhoria da fração orgânica do solo ao longo do tempo de adoção, se aproximando às condições da MN. Já as áreas de PP e RPPN evidenciaram que o manejo inadequado favorece na diminuição da qualidade edáfica.

Abstract This study quantified the C content of the chemical and physical fractions of SOM in different management systems in an Argisoil of sandy texture. The study was carried out in a reference area of Native Forest (NF), and in three managed areas: Permanent Pasture (PP), No-Tillage System (NTS) and an area of Private Natural Heritage Reserve (PNHR) in the process of natural regeneration. Soil samples were collected in the layers 0.0-0.05, 0.05-0.10 and 0.10-0.20 m. We assessed the soil density (Sd), total organic carbon (TOC) content, chemical fractionation of SOM with determination of the C contents of the fulvic acids (FA), humic acids (HA) and humin (HUM), with subsequent calculations of the HA/FA ratios, AE/HUM, stock (StockC), physical granulometric fractionation and determination of C contents of particulate organic matter (C-POM) and carbon management index (CMI). Higher TOC contents were observed for the NF area. The C-HA and C-HUM contents were higher in the NF and NTS. NF showed higher C-POM levels in all layers evaluated. For the C-MOM, the NTS area was superior to the other managed areas. The managed areas had lower StockPOM values than the NF. The managed areas had lower CMI values in relation to NF. The NTS area showed that, even in crop succession, it contributes to the improvement of the soil organic fraction over the adoption time. On the other hand, the areas of PP and PNHR showed that inadequate management favors the reduction of edaphic quality.

Resumo Objetivo Objetivamos caracterizar a estrutura da comunidade fitoplanctônica e identificar os principais fatores ambientais que impulsionam a comunidade em cinco reservatórios construídos na região do alto rio Paraná. Métodos O fitoplâncton e as variáveis ambientais foram coletados na região lacustre do reservatório, entre novembro de 2013 e novembro de 2014, com intervalo entre as coletas de 3 a 6 meses. A riqueza e a biomassa da comunidade fitoplanctônica foram utilizadas como resposta à variabilidade ambiental espacial e temporal dos reservatórios. Os dados das variáveis ambientais foram analisados por meio de uma Análise de Componentes Principais (PCA). Foram realizadas Análises de Escalonamento Multidimensional Não-Métrico (NMDS) aos dados de riqueza e biomassa da comunidade fitoplanctônica. Resultados Identificamos 80 táxons distribuídos em 11 classes, dos quais Cyanobacteria e Chlorophyceae foram os mais representados. Não observamos variação temporal significativa para variáveis ambientais ou atributos da comunidade fitoplanctônica, o que pode estar relacionado à prolongada estiagem neste período no sudeste brasileiro. Os maiores valores de riqueza e biomassa fitoplanctônica foram encontradas em Três Irmãos (Rio Tietê), reservatório localizado na bacia mais antropizada do país. Cianobactérias e dinoflagelados dominaram a biomassa em todos os reservatórios durante o período estudado. Os reservatórios de Ilha Solteira, Jupiá e Porto Primavera apresentaram tendência de diminuição nos valores de riqueza e biomassa fitoplanctônica, sendo que os reservatórios construídos em série no rio Paraná provavelmente possuem forte interdependência, de acordo com o conceito do CRCC. Conclusões A variação espacial dos atributos fitoplanctônicos foi influenciada principalmente pela posição ocupada pelo reservatório na bacia hidrográfica, tempo de retenção de água (TR) e concentrações de nutrientes em cada reservatório.

Abstract Aim We aimed to characterize the structure of the phytoplankton community and identify the main environmental factors driving the community in five reservoirs constructed in the region of the high Paraná River. Methods The phytoplankton and environmental variables were collected at the lacustrine region of the reservoir, between November 2013 and November 2014, with interval between collections ranged from 3 to 6 months. The richness and biomass of the phytoplankton community were measured as a response to the spatial and temporal environmental variability. Data from environmental variables was analyzed by Principal Component Analysis (PCA). Non-Metric Multidimensional Scaling Analyzes (NMDS) were performed on the richness and biomass data of the phytoplankton community. Results We identified 80 taxa distributed in 11 taxonomic classes, from which Cyanobacteria and Chlorophyceae were best represented. We did not observe significant temporal variation for either environmental variables or attributes of the phytoplankton community, which may be related to the prolonged drought in this period in the Brazilian Southwest. Higher phytoplankton richness and biomass were found in the Três Irmãos (Tiete River), reservoir located in the most anthropized basin in the country. Cyanobacteria and dinoflagellates dominated the biomass in all reservoirs during the studied period. The Ilha Solteira, Jupiá and Porto Primavera reservoirs showed a tendency to decrease in the values of phytoplankton richness and biomass, and the reservoirs built in series in the Paraná River probably have strong interdependence, according to the CRCC concept. Conclusions Spatial variation in phytoplankton attributes was influenced mainly by the position occupied by the reservoir in the hydrographic basin, water retention time (RT) and nutrient concentrations in each reservoir.

Resumo: O estudo analisou a mudança de crenças de uma graduanda sobre o ensino dos esportes ao longo da formação inicial em Educação Física, em uma universidade pública do sul do Brasil. Neste estudo qualitativo, longitudinal, de caráter descritivo e interpretativo, investigou-se o caso único de uma graduanda, a partir de procedimentos de entrevista semiestruturada, observação sistemática, estimulação de memória e a técnica de Análise de Conteúdo. Os resultados revelaram que, ao longo da formação inicial, a graduanda modificou suas crenças relacionadas aos propósitos, estratégias e organização do conteúdo, por meio de diferentes processos de mudança conceitual. Essas evidências mostram que a aprendizagem profissional, compreendida na perspectiva da mudança conceitual, é um processo ativo, longo, lento e gradual, que envolve a revisão contínua de crenças.

Abstract: The study analyzed the change in beliefs of an undergraduate student about sports teaching during her initial training in Physical Education at a public university in southern Brazil. In this qualitative, descriptive, and interpretive longitudinal study, the unique case of an undergraduate student was investigated, based on semi-structured interview procedures, systematic observation, memory stimulation and Content analysis technique. The results revealed that throughout her initial training, the undergraduate student changed her beliefs related to the purposes, strategies, and organization of content through different processes of conceptual change. This evidence shows that professional learning, comprehensive from the perspective of conceptual change, is an active, long, slow, and gradual process that involves a continuous revision of beliefs.

Resumen: El estúdio analizó el cambio de creencias de un estudiante de posgrado sobre la enseñanza del deporte durante la formación inicial en Educación Física en una universidad pública del sur de Brasil. En este estudio longitudinal, cualitativo, de carácter descriptivo e interpretativo, se investigó el caso único de un estudiante, con base em procedimientos de entrevista semiestructurada, observación sistemática, estimulación de la memoria y técnica. Contenido Análisis. Los resultados revelaron que durante la formación inicial, los estudiantes cambiaron sus creencias relacionadas con los propósitos, estrategias y organización del contenido, a través de diferentes procesos de cambio conceptual. Esta evidencia muestra que el aprendizaje profesional, entendido desde la perspectiva del cambio conceptual, es un proceso activo, largo, lento y gradual, que implica la revisión continua de creencias.

OBJECTIVE: This study aimed to assess miRNA-195 expression in the tumor tissues from a cohort of Brazilian female breast cancer patients undergoing neoadjuvant chemotherapy (NAC) and evaluate its correlation with various clinicopathological markers. METHODS: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate the miRNA-195 expression in tumor tissues from a cohort of female breast cancer patients undergoing NAC. This expression was then correlated with the occurrence of several distinct breast cancer molecular subtypes and other clinicopathological variables. RESULTS: A total of 55 patients were included in this study, 28 (50.9%) of whom were treated using NAC. Tumor miRNA-195 expression was suppressed in breast cancer patients, regardless of their exposure to systemic treatments, histological grade, size, nodal status, and tumor-node-metastasis (TNM) staging. This was more pronounced in luminal and triple-negative patients, and patient’s response to NAC was correlated with an increase in miRNA-195 expression. CONCLUSION: miRNA-195 is downregulated in the tumor tissues of Brazilian breast cancer patients regardless of NAC exposure; this reinforces its role as a tumor suppressor and a potential biomarker for chemotherapy response.