ABSTRACT: Polioencephalomalacia (PEM) is a nervous disease that affects ruminants, has a worldwide distribution and causes significant economic losses. PEM is abundantly described and studied in cattle and small ruminants, but there is little information about the disease in buffaloes, especially in Brazil. The objective of this work is to describe the clinical and pathological aspects of an outbreak of PEM in buffaloes in a feedlot in the state of Pará, Brazil. The outbreak occurred in a flock of 76 Murrah buffaloes, with an average age of five months, in a feedlot. Twenty-eight of these buffaloes became ill, and thirteen showed neurological signs and died. Two buffaloes underwent necropsy. The brain and fragments of various abdominal and thoracic cavity organs were fixed in 10% formalin and sent for histopathological examination. In the frontal, parietal, and occipital telencephalic lobes, the convolutions were flattened and yellowish, and when cut, there was a clear line of demarcation between the superficial and deep layers. Histologically, the intermediate layers of the cerebral cortex showed mild to moderate vacuolization of the neuropil, moderate enlargement of the perivascular, perineuronal, and periastrocyte spaces, moderate swelling and edema of astrocytes, and a large number of necrotic neurons. The work demonstrates that, despite being poorly documented in Brazil, PEM should be considered in the differential diagnosis of neurological syndromes in buffaloes. ABSTRACT (PEM ruminants losses Brazil Pará 7 months Twentyeight Twenty eight ill died necropsy 10 examination frontal parietal lobes yellowish cut Histologically neuropil perivascular perineuronal spaces astrocytes neurons 1

RESUMO: Polioencefalomalacia (PEM) é uma doença nervosa complexa que afeta ruminantes, tem distribuição mundial e causa perdas econômicas significativas. A PEM é abundantemente descrita e estudada em bovinos e pequenos ruminantes, porém há poucas informações sobre a doença em bubalinos, especialmente no Brasil. O objetivo deste trabalho é descrever os aspectos clínicos e patológicos de um surto de PEM em búfalos em um confinamento no estado do Pará, Brasil. O surto ocorreu em um lote de 76 búfalos da raça Murrah, com idade média de cinco meses, em um sistema de confinamento. Vinte e oito desses bubalinos adoeceram e treze apresentaram sinais neurológicos e morreram. Dois bubalinos foram submetidos à necropsia. O encéfalo e fragmentos de diversos órgãos da cavidade abdominal e torácica foram fixados em formol a 10% e enviados para exame histopatológico. Nos lobos frontal, parietal e occipital do telencéfalo as circunvoluções estavam achatadas e amareladas e ao corte havia uma clara linha de demarcação entre as camadas superficial e profunda. Na histologia as camadas intermediárias do córtex cerebral apresentavam vacuolização leve a moderada do neurópilo, aumento moderado dos espaços perivascular, perineuronal e periastrocitário, tumefação e edema moderados de astrócitos e grande número de neurônios necróticos. O trabalho demonstra que, apesar de pouco documentada no Brasil, a PEM deve ser considerada no diagnóstico diferencial das síndromes neurológicas em bubalinos. RESUMO (PEM ruminantes significativas Brasil Pará 7 Murrah meses morreram necropsia 10 histopatológico frontal profunda neurópilo perivascular periastrocitário necróticos 1

ABSTRACT: Anticarsia gemmatalis (Lepidoptera: Erebidae) is distributed in tropical and subtropical regions of America, and is an important pest of Fabaceae, such as: soybean, peanut, common bean, cowpea, pea, chickpea and kudzu. In soybean, the velvet bean caterpillar is important due to the plant defoliation in their larval stage. This study evaluated soybean cultivars as source of resistance to A. gemmatalis by antixenosis (attractiveness and non-preference for feeding) and antibiosis (biological parameters of the insect). The parameters evaluated were: antixenosis: attractiveness and non-preference, dry mass consumed and attractiveness index and antibiosis: duration of larval and pupal stages, adult longevity, total cycle, larval and pupal weights and larval, pupal and total viability. Considering antixenosis and antibiosis the least suitable cultivars for A. gemmatalis were found to be BRS 8383 IPRO, BRS 1074 IPRO, BRS 1061 IPRO, BRS 7180 IPRO, BRS 9383 IPRO, BRS 8980 IPRO and BRS 1003 IPRO due to high mortality in the larval phase. The cultivars BRS 523 and BRS 543 RR “block technology” suggest displays antixenosis and or antibiosis to A. gemmatalis. These cultivars can be used by soybean producers in combination with other control tactics in soybean IPM. ABSTRACT Lepidoptera (Lepidoptera Erebidae America Fabaceae peanut cowpea pea kudzu stage A nonpreference non preference feeding biological insect. insect . insect) nonpreference, preference, stages longevity cycle viability 838 107 106 718 938 898 100 phase 52 54 block technology IPM 83 10 71 93 89 5 8 1 7 9

RESUMO: Anticarsia gemmatalis (Lepidoptera: Erebidae) está distribuída nas regiões tropicais e subtropicais da América, trata-se de importante praga de Fabaceae, como: soja, amendoim, feijão, feijão-caupi, ervilha, grão de bico e kudzu. Na soja, a lagarta-da-soja é importante devido à desfolha da planta em sua fase larval. O objetivo deste trabalho foi avaliar cultivares de soja como fonte de resistência a A. gemmatalis por antixenose (atratividade e não-preferência para alimentação) e antibiose (parâmetros biológicos do inseto). Os parâmetros avaliados foram: antixenose: atratividade e não-preferência, massa seca consumida e índice de atratividade e antibiose: duração dos estágios larval e pupal, longevidade de adultos, ciclo total, peso larval e pupal e viabilidade larval, pupal e total. Na antixenose e antibiose os cultivares menos favoráveis a A. gemmatalis foram: BRS 8383 IPRO, BRS 1074 IPRO, BRS 1061 IPRO, BRS 7180 IPRO, BRS 9383 IPRO, BRS 8980 IPRO e BRS 1003 IPRO devido à alta mortalidade larval. Os cultivares BRS 523 e BRS 543 RR “Tecnologia block” apresentaram antixenose e/ou antibiose a A. gemmatalis. Essas cultivares podem serem utilizadas por produtores de soja em combinação com outras táticas de controle no MIP. RESUMO Lepidoptera (Lepidoptera Erebidae América tratase trata se Fabaceae amendoim feijão feijãocaupi, feijãocaupi caupi, caupi feijão-caupi ervilha kudzu lagartadasoja lagarta A nãopreferência não preferência alimentação inseto. inseto . inseto) foram nãopreferência, preferência, adultos total 838 107 106 718 938 898 100 52 54 Tecnologia block eou ou MIP 83 10 71 93 89 5 8 1 7 9

RESUMO Este estudo teve como objetivo analisar as características físicas dos ovos de galinhas caipiras var. Suras. Foram analisados um total de 100 ovos, onde diversos parâmetros físicos, tais como pesagem do ovo inteiro, medição do diâmetro equatorial e altura do ovo, classificação da cor da casca, miragem para verificação da presença de fendas na casca, determinação da altura da câmara de ar, classificação da cor da gema, verificação da presença de manchas de carne e manchas de sangue, medição da largura e comprimento do albúmen, medição do diâmetro da gema, pesagem da gema, e pesagem da casca. Foram posteriormente determinados o peso do albúmen por diferença entre o peso do ovo e pesos da casca e gema, determinação percentual dos diferentes constituintes do ovo, shape index (índice de forma) e shell index (índice de casca). A análise estatística foi realizada pelo GLM programa SAS (SAS Institute). O resultado comparando as médias de cada parâmetro estudado demonstrou que os ovos da variedade Sura em ambas as classes comerciais (Grande e Pequenos), possuem formato mais arredondado, com casca marrom clara e gema mais alaranjada. Portanto, os parâmetros avaliados confirmam que os ovos provenientes de galinhas var. Suras possuem características que podem favorecer nichos de mercado e assim incentivar a produção destas aves, mantendo a biodiversidade e contrariando a extinção das mesmas. var 10 físicos inteiro ar sangue índice forma . casca) Institute. Institute Institute) Grande Pequenos, Pequenos , Pequenos) arredondado alaranjada Portanto aves mesmas 1

ABSTRACT This study aimed to analyze the physical characteristics of eggs from free-range hens of the Sura variety. One hundred eggs underwent assessment of various physical parameters, namely, whole egg weight, equatorial diameter, egg height, shell color, presence of cracks via candling, air chamber height, yolk color, presence of flesh and blood spots, albumen width and length, yolk diameter, yolk weight, and shell weight. Additional analyses included determining the albumen weight (calculated by subtracting the weights of the shell and yolk from the total egg weight), percentages of egg components, shape index, and shell index. Statistical analyses were conducted using GLM SAS software (SAS Institute). The results revealed distinct physical traits; eggs from the Sura variety, regardless of size class (large or small), typically have a more rounded shape, light brown shells, and more orange yolks. These findings confirm that Sura variety eggs possess unique characteristics that could appeal to specific market niches, potentially promoting the production of these birds, supporting biodiversity, and aiding in the prevention of their extinction. freerange free range parameters namely diameter height color candling spots length calculated , weight) components index Institute. Institute . Institute) traits large small, small small) shells yolks niches birds biodiversity extinction

ABSTRACT Purpose: The aim of this study focused on the elaboration and validation of an organizational aesthetic perception scale (AESCATScale) to be used in understanding individuals’ perceptions of organizational life. Design/methodology/approach: We carried out a literature review, exploratory interviews, two separate surveys, and multivariate analyses. The scale was validated through an application with a large sample of college professors. Findings: Our outcomes consisted of a multidimensional construct model for the perception of organizational aesthetics (AESCATScale), with seven dimensions and 20 items. According to our analysis, the organizational aesthetics construct is consistent and may be the basis for future studies on the subject. Research limitations/implications: We highlight three peculiar limitations to the research: a) the subjective nature of the theme, b) the lack of comprehension of some categories by respondents and c) the sample used was chosen for its accessibility and to have been restricted to college professors of a single state in Brazil. Practical implications: The AESCATScale may reveal the sensitive logic underlying several organizational practices in the workplace based on a sensitive point of view. Also, it can be a valuable tool to aid in a manager’s decision-making in situations of change. Originality/value: This study introduces a differentiated approach to organizational management that can sometimes be missed due to its abstract nature. Therefore, it introduces a novelty research tool that can be used in corporate and academic environments. Purpose (AESCATScale individuals life Design/methodology/approach Designmethodologyapproach Design methodology review interviews surveys analyses Findings AESCATScale, , 2 items analysis subject limitations/implications limitationsimplications implications theme b c Brazil view Also managers manager s decisionmaking decision making change Originality/value Originalityvalue Originality value Therefore environments

RESUMO Objetivo: O objetivo deste estudo centrou-se na elaboração e validação de uma escala de percepção estética organizacional (AESCATScale) para ser usada na compreensão das percepções dos indivíduos sobre a vida organizacional. Metodologia: Para tanto, realizamos revisão bibliográfica, entrevistas exploratórias, aplicação de surveys e técnicas de análise multivariada. A escala foi validada por meio de aplicação com professores de ensino superior Resultados: Nossos resultados consistiram em um modelo de construto multidimensional para percepção da estética organizacional (AESCATScale), com sete dimensões e vinte itens. Conforme a análise, o construto se mostrou consistente e pode ser tomado como base para evoluções posteriores do estudo. Limitações/implicações da pesquisa: Destacamos três limitações peculiares à pesquisa: a) o caráter subjetivo do tema, b) a falta de compreensão de algumas categorias pelos respondentes e c) a amostra utilizada foi escolhida por sua acessibilidade e por ter sido restrita a professores universitários de um único estado do Brasil. Implicações práticas: A AESCATScale pode revelar a lógica sensível subjacente a várias práticas organizacionais no local de trabalho de um ponto de vista sensível. Além disso, pode ser uma ferramenta útil para auxiliar na tomada de decisão de um gerente em situações de mudança. Originalidade/valor: Este estudo apresenta uma abordagem diferenciada para a gestão organizacional que às vezes pode ser perdida devido à sua natureza abstrata. Portanto, apresenta uma ferramenta de pesquisa inovadora que pode ser usada tanto em ambientes corporativos quanto acadêmicos. Objetivo centrouse centrou (AESCATScale Metodologia bibliográfica exploratórias multivariada Resultados AESCATScale, , itens Limitaçõesimplicações Limitações implicações tema b c Brasil disso mudança Originalidade/valor Originalidadevalor Originalidade valor abstrata Portanto acadêmicos

ABSTRACT Background: The aim of this research was to investigate the efficiency of methods to overcome primary dormancy and biochemical processes associated with germination of Erythrina falcata Benth seeds. Seeds were submitted to dormancy overcoming treatments and the water uptake pattern was analysed. We then evaluated the activity of the antioxidant enzymes SOD, CAT, and APX, and quantified lipid peroxidation levels, hydrogen peroxide content and total protein content. The experiments were performed in a completely randomized design and the statistical analysis used was Scott-Knott test at 5% probability. Results: Mechanical scarification with sandpaper leads to approximately 94% of germination in E. falcata, while control non-scarified seeds show only approximately 37% of germination. Treatments with hot water led to high mortality in E. falcata seeds. Rapid water absorption was observed in the first 18 hours of imbition when the seeds were scarified with sandpaper, and radicle emergence was observed after 36 hours. During imbibition there was an increase of antioxidant enzyme activity and a decrease in lipid peroxidation and H2O2 production, suggesting an efficient mechanism for regulating imbibition damage. A decrease in the total protein content was observed during germination of E. falcata seeds. Conclusions: Mechanical scarification is an efficient method for breaking dormancy of E. falcata seeds. During germination of E. falcata seeds there is a reduction in the production of H2O2 and lipid peroxidation, and an increase in the activity of antioxidant enzymes. The total protein content decreased along the germination time. Background analysed SOD CAT APX levels ScottKnott Scott Knott 5 probability Results 94 E nonscarified non 37 1 3 HO H O H2O damage Conclusions time 9

SUMMARY OBJECTIVE: This study aimed to evaluate the quality of life of patients with endometriosis before and after surgical treatment. METHODS: An observational, longitudinal, and prospective study was conducted with 102 women with pelvic pain and endometriosis that was unimproved by clinical treatment and indicated for surgical treatment. The patients’ quality of life was assessed using the 30-item Endometriosis Health Profile (EHP-30) questionnaire before and 3 and 6 months after surgery. The statistical tests were analyzed using the Statistical Package for Social Sciences version 17.0, and the Friedman test was used. RESULTS: There was a reduction in EHP-30 scores 3 and 6 months after surgery compared to before surgery, as well as 6 months after surgery compared to 3 months after surgery, in the central questionnaire (PART 1) and in Sections A, B, C, E, and F (p<0.0001). For Section D, there was a reduction in scores 6 months after surgery compared to before surgery (p<0.0001). CONCLUSION: Surgical treatment of endometriosis improves quality of life in several areas assessed by the EHP-30 questionnaire. OBJECTIVE METHODS observational longitudinal 10 30item item 30 EHP30 EHP (EHP-30 170 17 0 17.0 used RESULTS EHP-3 PART 1 A B C E p<0.0001. p00001 p p<0.0001 . 0001 (p<0.0001) D CONCLUSION EHP3 (EHP-3 17. EHP- p0000 p<0.000 000 (p<0.0001 (EHP- p000 p<0.00 00 (p<0.000 (EHP p00 p<0.0 (p<0.00 p0 p<0. (p<0.0 p<0 (p<0. p< (p<0 (p< (p

Abstract Napier grass is a perennial tropical forage that is used in beef and dairy production systems. Despite its significance in animal nutrition, molecular information available, such as microsatellite or simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers, is limited. Using an assembled transcriptome, 50 novel SSR markers were developed, of which 21 were found to be polymorphic. These polymorphic markers were tested for DNA fingerprinting of Embrapa cultivars, five of which revealed distinct allele patterns for cultivar identification. SSR markers 05, 17, and 44 identified a unique pattern in the BRS Kurumi cultivar. The BRS Capiaçu cultivar was identified using SSR markers 17, 43, and 44. The Pioneiro cultivar exhibited a rare fragment amplification pattern using SSR marker 46, while SSR marker 44 revealed a distinct allele in the BRS Canará cultivar. SSR marker panels could be utilized as DNA fingerprinting tools to assist in cultivar identification.

Abstract We present this manuscript as a methodological approach and general guidelines for geochemical mapping and background/baseline projects for environmental assessment in tropical areas. A case study was carried out in the Itacaiúnas River watershed (IRW), Eastern Amazon, to fill in a gap in knowledge on the distribution of chemical elements, particularly those potentially toxic, in the near-surface environment of the area. The high-impact results of this research project revealed the need for similar scientific investigation across the globe with the implementation of a systematic methodology. The study shows, for example, the importance of well-planned field activities, multi-medium sampling, analytical methods, laboratory procedures, database construction, and general aspects of data processing and statistical treatment. The importance of this contribution is that it can be used as a reference in support of geospatial analysis in research within the scope of geochemical mapping and background-baseline projects. The database is accessible through a web-based geographic information system front-end; a Geochemical Atlas of the IRW will be available as soon as possible. backgroundbaseline background baseline areas IRW, , (IRW) Amazon elements toxic nearsurface near surface area highimpact high impact methodology shows example wellplanned well planned activities multimedium multi medium sampling methods procedures construction treatment webbased web based frontend front end front-end possible (IRW

ABSTRACT Purpose: To demonstrate through a controlled study whether the use of tranexamic acid in bariatric surgeries is effective for bleeding control. Methods: Prospective, comparative, and double-blind study performed with patients from 18 to 65 years old submitted to bariatric surgery. The selected patients received venous tranexamic acid (TXA) during the induction of anesthesia or not (CG). The anesthesia and thromboprophylaxis protocols were similar among the groups. For statistical analysis, the χ2 and analysis of variance tests were performed at a significance level of p < 0.05, using the statistical program SPSS 21.0®. Results: Sixty-one patients were included in the study, 31 in the control group and 30 in the TXA group (GTXA). In the intraoperative period, the bleeding volume was greater in the CG than in the GTXA. In the postoperative period, the tranexamic acid group had a higher value hematocrit, absence of surgical reoperations due to bleeding complications, and shorter hospitalization time than the control group. Conclusions: The use of tranexamic acid was effective in reducing bleeding rates and of hospital stay length, in addition to demonstrating the clinical safety of its use, for not having been associated with any thromboembolic events.

Abstract We report the anesthetic management with combined spinal-epidural in a patient with limb-girdle muscular dystrophy type 2A, submitted to abdominoplasty and liposuction. The patient had onset of symptoms at 8 years old, diagnosed by muscular biopsy, presenting muscle weakness in the scapular and pelvic girdles, with reduced mobility. We performed monitorization with noninvasive blood pressure, oximeter, thermometer, and electrocardiogram. In the postoperative period, she showed no clinical signs of rhabdomyolysis, myotonia, or adverse effects, maintaining hemodynamic stability. The anesthesia technique allowed spontaneous ventilation, monitoring of clinical parameters close to physiological conditions and used smaller doses of medication, reducing related risks.

RESUMEN Suponiendo que existe un alto acuerdo entre los pedólogos y una aplicación de smartphone en la descripción del color del suelo, el objetivo fue comparar las percepciones de los pedólogos y una aplicación en la obtención del color de un Argissolo [Lixisol] (horizontes A, E y B). Fueron recolectados diez agregados de cada horizonte. En un solo día, en las mismas condiciones de iluminación, tres podólogos describieron los componentes del color (matiz, valor y croma) de cada agregado (suelo seco y húmedo) utilizando la tabla de color Munsell. Cada uno de los diez agregados, de cada horizonte, fue fotografiado (secuencia de suelo seco y húmedo) con la cámara de un smartphone Motorola Moto G4 Plus. La distancia de la cámara a los agregados fue de 25 ± 5 cm. Además, cada agregado se colocó en una hoja blanca de papel A4 (fondo). La aplicación utilizada fue Soil Analysis Pro. El porcentaje de concordancia entre los pedólogos y la aplicación se obtuvo en relación con el matiz, valor y croma. Los datos se sometieron a análisis de varianza en un diseño completamente al azar, con diez repeticiones, utilizando el software Action Stat®. Se llegó a la conclusión de que el acuerdo entre los pedólogos y la aplicación del smartphone era medio en valor y croma y bajo en valor. En suelo seco, existe un alto nivel de acuerdo entre los pedólogos y la aplicación, principalmente en la percepción de matiz y croma. Por lo tanto, la aplicación para smartphone tiene el potencial de usarse en descripciones rutinarias del color del suelo.

ABSTRACT Based on the hypothesis that there is a high agreement between pedologists and a smartphone application in the assessment of soil color, this study aimed to compare the perceptions of pedologists and an application in obtaining the color of an Argissolo [Lixisol] (A, E, and B horizons). Ten aggregates of each horizon were collected. In a single day, under the same lighting conditions, three pedologists described the color components (hue, value, and chroma) of each aggregate (dry and moist soil) using the Munsell soil color chart. Each one of the ten aggregates, from each horizon, was photographed (dry and moist soil sequence) using the camera of a Motorola Moto G4 Plus smartphone. The distance of the camera to the aggregates was 25 ± 5 cm. Also, each aggregate was placed on a white sheet of A4 size paper (background). The application used was Soil Analysis Pro. The percentage of agreement between pedologists and application was obtained concerning hue, value, and chroma. The data were subjected to analysis of variance, in a completely randomized design, with ten replicates. Action Stat® software was used for statistical analysis. It was concluded that the agreement between pedologists and the smartphone application was medium for hue and chroma and low for value. For the dry soil condition, there is a high agreement between pedologists and the smartphone application, especially in the perception of hue and chroma. Thus, the smartphone application has the potential to be used in routine descriptions of soil color.

Abstract While the amount of telecommunications services grows rapidly in the whole world, humans get potentially more exposed to Non-Ionizing Radiation (NIR) from a number of different sources. Measurements of NIR levels are relevant in order to compare the results with national and international standards, aiming at the preservation of human health. Thus, it is of great interest to explore a variety of topics regarding this subject. Based on this need, this paper has a number of goals, including monitoring radiation levels in an everyday situation, an investigation of how national and international regulations organs address NIR levels and a demonstration of scientific production trends regarding this topic. The work also presents a bibliometric study about the main scientific productions and trends related to NIR measurements, with focus on the field of Telecommunications. The analysis is based on the databases of Web of Science (WOS) and Scopus, computing publications in the last 10 and 3 years, showing a trend evaluation. Among the main results from this exploratory investigation, there is an exponential growth in the number of publications about NIR, ranging from research in physics to medicine. The way research contribution in different countries is also shown, in order to support the relevance of the topic in defining new tendencies for new investigations. Furthermore, the NIR exposure limits and measurement criteria are presented both in Brazilian and in international norms, demonstrating how different national regulations can be when compared to international guidelines. Another contribution is a case study in Natal, Brazil, where NIR levels were monitored in four distinct locations over a period of 24 hours each and compared to the current regulations. The result is a quantitative analysis of the amount of radiation that some populations might be exposed to in distinct moments of the day. Measurements were carried out in the proximity of an antenna spot, since it could represent a source of high NIR levels (a worst case of human exposure). This campaign differs from older studies by including a more intense usage of microwaves frequencies due to operating 4G and pre-5G systems in Brazil. Finally, a statistical study involving the measurements is conducted, concluding the analysis of how some population groups might be affected by NIR.

Resumo O presente trabalho realizou a coleta de amostras de sangue total e carrapatos de 57 capivaras da região, com o intuito de pesquisar bactérias do gênero Rickettsia spp., a partir de métodos de diagnóstico molecular (PCR) e sorológicos (RIFI). Nenhuma amostra de sangue das capivaras foi positiva na PCR. Dentre as 55 amostras de soro testadas por RIFI, 53 (96,3%) apresentaram soro-reação para Rickettsia spp. Dessas 53 amostras, 21 (39,6%) demonstraram o antígeno de R. bellii como provável antígeno envolvido em reação homóloga (PAERH); e duas (3,8%) demonstraram o antígeno de R. parkeri. Foram amostrados 173 carrapatos identificados como Amblyomma sculptum e 410 como Amblyomma dubitatum, além de nove larvas (Amblyomma spp.). Entre os carrapatos, 231 foram submetidos à extração de DNA e PCR para pesquisa de Rickettsia. Foram encontradas evidências moleculares de R. bellii em 25/108 (23,1%) e Rickettsia sp. “strain” Cooperi em 2/108 (1,9%) das amostras de A. dubitatum testadas. Os resultados sugerem uma maior exposição à R. bellii nas populações de capivaras da região, além de uma maior quantidade de carrapatos identificados como A. dubitatum. Isso pode estar relacionado ao que configura a região do Distrito Federal como não endêmica para a febre maculosa brasileira.

Abstract In this study, whole blood samples and ticks were collected from 57 capybaras in recreational areas in the Federal District, Brazil, aiming to investigate the presence of Rickettsia spp. using polymerase chain reaction (PCR) and indirect immunofluorescence (IFAT) assays. None of the capybara blood samples yielded rickettsial DNA by PCR. Among 55 capybara serum samples tested by IFAT, 53 (96.3%) reacted to Rickettsia spp. Among these, 21 (39.6%) identified the R. bellii antigen as the probable antigen involved in a homologous reaction (PAIHR), whereas 2 (3.8%) identified the R. parkeri antigen. Ticks collected from capybaras were identified as 173 Amblyomma sculptum and 410 A. dubitatum, in addition to nine Amblyomma spp. larvae. A sample of 231 ticks was subjected to DNA extraction and PCR for Rickettsia species. None of 122 A. sculptum yielded rickettsial DNA. Molecular evidence of R. bellii was found in 25/108 (23.1%) and of Rickettsia sp. strain Cooperi (R. parkeri-like agent) in 2/108 (1.9%) of the A. dubitatum samples. These results suggest a greater exposure to R. bellii in these capybara populations, in addition to a more significant number of A. dubitatum, which might characterize the Federal District region as not endemic for Brazilian spotted fever.

RESUMO Compreender a dinâmica hidrossedimentológica dos rios tropicais é um desafio, principalmente na Amazônia, devido às suas áreas remotas e de difícil acesso. Este estudo foi baseado em dados coletados em 16 seções hidrossedimentológicas de controle distribuídas nas 6 sub-bacias que compõem a bacia hidrográfica do Rio Itacaiúnas (BHRI), com 4 campanhas anuais (enchente, cheia, vazante e seca) entre 2015 e 2019, objetivando a construção e comparação da curva-chave de sedimentos e produção de sedimentos. Os dados na foz da bacia revelaram que o período chuvoso é responsável por 93% das descargas líquidas com médias de 1460,88 m3/s e por 98% das descargas de sedimentos em suspensão com média de 5864,15 ton/dia. As Concentrações de sedimentos em suspensão (CSSs) são baixas a moderadas (50 a 150 mg/l). As análises das curvas obtidas com todos os dados apresentaram R2 (0,92 a 0,99) maiores do que utilizando apenas os valores da estação chuvosa ou seca, mostrando um bom ajuste da equação de potência aos dados QSS e Q para todas as seções estudadas. Os maiores valores do coeficiente a e b indicam áreas de maior produção de sedimentos e desmatamento e áreas com novas fontes de sedimentos e floresta preservada.

ABSTRACT Understanding the hydrosedimentological dynamics of tropical rivers is a challenge in the Amazon due to its remote and difficult-to-access areas. This study was based on data collected from 16 hydrosedimentological control sections in the 6 subbasins that make up the Itacaiúnas River Watershed (IRW), with 4 annual campaigns (high water levels, rising water levels, falling water levels, low water levels) between 2015 and 2019, with the aim of constructing and comparing sediment rating curves and sediment yield. The data at the mouth of the IRW revealed that the rainy season is responsible for 93% of liquid discharges (Q) with an average of 1460.88 m3/s and for 98% of suspended sediment discharges (SSQ) with an average of 5864.15 tons/day. Suspended sediment concentrations (SSCs) are low to moderate (50 to 150 mg/l). The curves encompassing all the data showed R2 values (0.92 to 0.99) greater than the curves with only the values of the rainy or dry season, indicating a good fit of the power equation to the SSQ and Q data for all sections studied. Higher values of coefficients a and b show areas of greater sediment production and deforestation, as well as areas with new sources of sediment and preserved forest.