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E-learning as a strategy in dentistry in the context of COVID-19: a path to follow?
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MARQUES-MEDEIROS, Ana Carolina

; MARTINS, Renata Castro

; SOUZA, Leandro Napier de

; GOMEZ, Ricardo Santiago

; SILVA, Maria Elisa de Souza e

; MARTINS, Maria Auxiliadora Parreiras

; ABREU, Mauro Henrique Nogueira Guimarães de

Brazilian Oral Research

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2023, Volume 37 elocation e060

Resumo: En | Texto: En | PDF: En

Abstract The current study aims to assess the effectiveness of e-learning in compliance with the new biosafety recommendations in dentistry in the context of COVID-19 applied to the clinical staff of a dental school in Brazil. A quasi-experimental epidemiological study was carried out by means of a structured, pre-tested online questionnaire, applied before and after an educational intervention, using an e-learning format. After data collection, statistical tests were performed. A total of 549 members of the clinical staff participated in the study in the two collection phases, with a return rate of 26.9%. After the e-learning stage, a reduction was found in the reported use of disposable gloves, protective goggles, and surgical masks. The course had no impact on the staff’s knowledge concerning the proper sequence for donning PPE and showed 100% effectiveness regarding proper PPE doffing sequence. Knowledge about avoiding procedures that generate aerosols in the clinical setting was improved. Despite the low rate of return, it can be concluded that online intervention alone was ineffective in significantly improving learning about the new clinical biosafety guidelines. Therefore, the use of hybrid teaching and repetitive training is highly recommended.

https://doi.org/10.1590/1807-3107bor-2023.vol37.0060

Psychometric properties of the Brazilian Portuguese version of the UPPS-P impulsive behavior scale for children and adolescents
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Machado, Yuri de Castro

; Paula, Jonas Jardim de

; Malloy-Diniz, Leandro Fernandes

; Miranda, Débora Marques de

; Romano-Silva, Marco Aurélio

Jornal de Pediatria

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2023, Volume 99 Nº 6 Páginas 588 - 596

Resumo: En | Texto: En | PDF: En

Abstract Objective: To translate and validate the version for children and adolescents of the UPPS-P scale into Brazilian Portuguese. Method: After a five-step translation process, the final draft was submitted to a panel of 12 different specialties experts. Subsequently, the application of the scale was applied concomitantly with the "Swanson, Nolan, and Pelham Scale - Version IV" (SNAP-IV) and the "Child and Adolescent Behavior Inventory" (CABI) scale to analyze the correlation between them. Content Validity Index (CVI) and reliability were estimated by calculating internal consistency and analyzing its test-retest stability. Results: The items whose CVI was lower than 80% underwent a detailed analysis to verify the reason for the bad evaluation. Five items (3, 7, 11, 22 and 35) scored below 80% and were reassessed. There was high internal consistency in all parameters: Lack of premeditation (McDonald's omega = 0.806; Cronbach's alpha = 0.801), Negative Urgency (McDonald's omega = 0.838; Cronbach's alpha = 0.836), Sensation seeking (McDonald's omega = 0.826; Cronbach's alpha = 0.810), Lack of Perseverance (McDonald's omega = 0.800; Cronbach's alpha = 0.799) and Positive Urgency (McDonald's omega = 0.936; Cronbach's alpha = 0.934). A strong correlation was observed between UPPS-P features and impulsivity in behavioral assessments. Conclusions: The Brazilian Portuguese version of the UPPS-P scale is a good instrument to assess impulsivity in children and adolescents.

https://doi.org/10.1016/j.jped.2023.04.008

Staff Knowledge and Attitudes Towards COVID-19 New Biosafety Practices at a Brazilian Dental School
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Marques-Medeiros, Ana Carolina

; Martins, Renata Castro

; Silva, Maria Elisa Souza e

; Vilaça, Ênio Lacerda

; Souza, Leandro Napier de

; Martins, Maria Auxiliadora Parreiras

; Gomez, Ricardo Santiago

; Guimarães de Abreu, Mauro Henrique Nogueira

Pesquisa Brasileira em Odontopediatria e Clínica Integrada

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2022, Volume 22 elocation e210139

Resumo: En | Texto: En | PDF: En

ABSTRACT Objective To evaluate knowledge and attitudes towards biosafety recommendations during the COVID-19 pandemic at a Brazilian dental school. Material and Methods A cross-sectional study was performed in 2020 with the clinical staff of a Brazilian dental school. The whole clinical staff was sent pre-tested self-administered online questionnaires about knowledge and attitudes towards the recommendations for biosafety in dental settings in the context of the COVID-19 pandemic. Descriptive statistical analyses were carried out for proportion calculation. Results Disposable head covering caps, isolation gowns, and gloves were the most frequently reported personal protective equipment (PPE). The rates ranged from 52.9% to 88.5% for N95 respirators, from 68.6% to 92.6% for face shields, from 47.4% to 67.5% for conventional eye protection shields, and 45.1% to 77.4% for eye protection with solid side shields. Chlorhexidine gluconate was the most frequent mouthwash indicated before clinical dental care. The percentage of agreement to provide clinical care to patients with suspected COVID-19 varied from 23.5% to 50.0%. The percentage of respondents who agreed that bioaerosol-generating procedures should be avoided was higher than 74.5%. Less than 50% knew the correct sequence for doffing of PPE. Conclusion This study revealed important gaps in knowledge and attitudes towards prevention and control measures against infection in dental environments in the context of COVID-19, indicating the need for improvements.

https://doi.org/10.1590/pboci.2022.007

The influence of demography and referral medical specialty on the detection of autoantibodies to HEP-2 cells in a large sample of patients
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Santos, Wilton Ferreira Silva

; Cantuária, Ana Paula de Castro

; Félix, Daniele de Castro

; Nardes, Leandro Kegler

; Melo, Igor Cabral Santos de

Advances in Rheumatology

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2022, Volume 62 elocation 32

Resumo: En | Texto: En | PDF: En

Abstract Background: The prevalence of anti-cell autoantibodies detected by indirect immunofluorescence assay on HEp-2 cells (HEp-2-IIFA) increases with age and is higher in female sex. The number of medical specialties that use HEp-2-IIFA in the investigation of autoimmune diseases has increased lately. This study aimed to determine the prevalence and patterns of autoantibodies on HEp-2-IIFA according to demographics variables and referring medical specialties. Methods: A retrospective analysis of the HEp-2-IIFA carried out between January and June of 2017 was performed. The International Consensus on Antinuclear Antibodies Patterns (ICAP) and the Brazilian Consensus on Autoantibodies were used for patterns definition on visual reading of the slides. Anti-cell (AC) codes from ICAP and Brazilian AC codes (BAC) were used for patterns classification. Results: From 54,990 samples referred for HEp-2-IIF testing, 20.9% were positive at titer ≥ 1/80. HEp-2-IIFA positivity in females and males was 24% and 12%, respectively ( p < 0.0001). The proportion of positive results in the 4 age groups analyzed: 0–19, 20–39, 40–59, and ≥ 60 years was 23.3, 20.2, 20.1, and 22.8%, respectively ( p < 0.0001). Considering all positive sera (n = 11,478), AC-4 nuclear fine speckled (37.7%), AC-2 nuclear dense fine speckled (21.3%), BAC-3 nuclear quasi -homogeneous (10%) and mixed/composite patterns (8.8%) were the most prevalent patterns. The specialties that most requested HEp-2-IIFA were general practitioner (20.1%), dermatology (15%), gynecology (9.9%), rheumatology (8.5%), and cardiology (5.8%). HEp-2-IIFA positivity was higher in patients referred by rheumatologists (35.7% vs. 19.6%) ( p < 0.0001). Moderate (46.4%) and high (10.8%) titers were more observed in patients referred by rheumatologists ( p < 0.0001). We observed a high proportion of mixed and cytoplasmic patterns in samples referred by oncologists and a high proportion of BAC-3 (nuclear quasi -homogeneous) pattern in samples referred by pneumologists. Conclusions: One-fifth of the patients studied were HEp-2-IIFA-positive. The age groups with more positive results were 0–19 and ≥ 60 years. AC-4, AC-2, BAC-3 and mixed/composite patterns were the most frequent patterns observed. Rheumatologists requested only 8.5% of HEp-2-IIFA. Positive results and moderate to high titers of autoanti-bodies were more frequent in patients referred by rheumatologists.

https://doi.org/10.1186/s42358-022-00264-1

Elaboration of fracture prediction map using 2D digital image correlation - 2D CID
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Assis, Leandro Silva de

; Assis, Joaquim Teixeira de

; Pessoa, José Renato de Castro

; Tavares Júnior, Armando Dias

Revista IBRACON de Estruturas e Materiais

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2022, Volume 15 Nº 4 elocation e15403

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Este trabalho tem como objetivo apresentar uma metodologia para elaboração de um mapa de deformação em um corpo de prova de concreto de cimento Portland de modo a prever fraturas provocadas por tensão de compressão axial, utilizando técnica de Correlação Digital de Imagem - CID 2D. Para alcançar este objetivo, foram analisados 5 corpos de prova de concreto com resistência à compressão esperada aos 28 dias fck de 40 MPa que foram ensaiados por meio da norma da ABNT NBR 5739/2018 - ensaio de compressão de corpos de prova cilíndricos de concreto. Durante o ensaio foram adquiridas as imagens digitais necessárias no arranjo CID-2D. Posteriormente essas imagens foram processadas e os resultados interpretados estatisticamente. De acordo com o resultado de correlação de imagens obtido, constatou-se que 67% dos corpos de prova possuíam regiões acumuladoras de tensão que indicaram antecipadamente o local da ruptura, o que permitiu a elaboração de um mapa de previsão de fratura. Os resultados obtidos na pesquisa mostraram que a metodologia utilizada por meio do arranjo CID-2D conseguiu prever o local onde houve ruptura no corpo de prova.

Abstract This work aims to present a methodology for the elaboration of a deformation map in a Portland cement concrete specimen to predict fractures caused by axial compression stresses, using the technique of Digital Image Correlation - DIC 2D. For this purpose, 5 concrete specimens with compressive strength expected at 28 days fck of 40 MPa were analyzed, which were tested in the ABNT NBR 5739/2018 standard - compression test of cylindrical concrete specimens. During the test, the necessary digital images were acquired in the DIC-2D array. These images were subsequently processed, and the results interpreted statistically. According to the result of the correlation of images obtained, it was found that 67% of the specimens had regions of accumulation of stresses that indicated in advance the location of the rupture, which enabled the development of a fracture prediction map. The results obtained in the research showed that the methodology used by means of the DIC-2D arrangement was able to predict the place where the rupture in the specimens occurred.

https://doi.org/10.1590/s1983-41952022000400003

Recommendations by the Scientific Department of Neuroimmunology of the Brazilian Academy of Neurology (DCNI/ABN) and the Brazilian Committee for Treatment and Research in Multiple Sclerosis and Neuroimmunological Diseases (BCTRIMS) on vaccination in general and specifically against SARS-CoV-2 for patients with demyelinating diseases of the central nervous system
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Becker, Jefferson

; Ferreira, Lis Campos

; Damasceno, Alfredo

; Bichuetti, Denis Bernardi

; Christo, Paulo Pereira

; Callegaro, Dagoberto

; Peixoto, Marco Aurélio Lana

; Sousa, Nise Alessandra De Carvalho

; Almeida, Sérgio Monteiro De

; Adoni, Tarso

; Santiago-Amaral, Juliana

; Junqueira, Thiago

; Pereira, Samira Luisa Apóstolos

; Gomes, Ana Beatriz Ayroza Galvão Ribeiro

; Pitombeira, Milena

; Paolilo, Renata Barbosa

; Grzesiuk, Anderson Kuntz

; Piccolo, Ana Claudia

; D´Almeida, José Arthur Costa

; Gomes Neto, Antonio Pereira

; Oliveira, Augusto Cesar Penalva De

; Oliveira, Bianca Santos De

; Tauil, Carlos Bernardo

; Vasconcelos, Claudia Ferreira

; Kaimen-Maciel, Damacio

; Varela, Daniel

; Diniz, Denise Sisterolli

; Oliveira, Enedina Maria Lobato De

; Malfetano, Fabiola Rachid

; Borges, Fernando Elias

; Figueira, Fernando Faria Andrade

; Gondim, Francisco De Assis Aquino

; Passos, Giordani Rodrigues Dos

; Silva, Guilherme Diogo

; Olival, Guilherme Sciascia Do

; Santos, Gutemberg Augusto Cruz Dos

; Ruocco, Heloisa Helena

; Sato, Henry Koiti

; Soares Neto, Herval Ribeiro

; Cortoni Calia, Leandro

; Gonçalves, Marcus Vinícius Magno

; Vecino, Maria Cecilia Aragón De

; Pimentel, Maria Lucia Vellutini

; Ribeiro, Marlise De Castro

; Boaventura, Mateus

; Parolin, Mônica Koncke Fiuza

; Melo, Renata Brant De Souza

; Lázaro, Robson

; Thomaz, Rodrigo Barbosa

; Kleinpaul, Rodrigo

; Dias, Ronaldo Maciel

; Gomes, Sidney

; Lucatto, Simone Abrante

; Alves-Leon, Soniza Vieira

; Fukuda, Thiago

; Ribeiro, Taysa Alexandrino Gonsalves Jubé

; Winckler, Thereza Cristina D’ávila

; Fragoso, Yara Dadalti

; Nascimento, Osvaldo José Moreira Do

; Ferreira, Maria Lucia Brito

; Mendes, Maria Fernanda

; Brum, Doralina Guimarães

; Glehn, Felipe Von

Arquivos de Neuro-Psiquiatria

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Nov 2021, Volume 79 Nº 11 Páginas 1049 - 1061

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O DC de Neuroimunologia da ABN e o BCTRIMS trazem, nesse documento, as recomendações sobre vacinação da população com doenças desmielinizantes do sistema nervoso central (SNC) contra infecções em geral e contra o coronavírus da síndrome respiratória aguda grave 2 (SARS-CoV-2), causador da COVID-19. Destaca-se a gravidade do atual momento frente ao avanço da COVID-19 em nosso País, o que torna mais evidente e importante a criação de guia de referência para orientação aos médicos, pacientes e autoridades de saúde pública quanto à vacinação, meio efetivo e seguro no controle de determinadas doenças infecciosa. O DCNI/ABN e o BCTRIMS recomendam que os pacientes com doenças desmielinizantes do SNC (ex., EM e NMOSD) sejam constantemente monitorados, quanto a atualização do seu calendário vacinal, especialmente, no início ou antes da mudança do tratamento com uma droga modificadora de doença (DMD). É importante também salientar que as vacinas são seguras e os médicos devem estimular o seu uso em todos os pacientes. Evidentemente, deve ser dada especial atenção às vacinas com vírus vivos atenuados. Por fim, é importante que os médicos verifiquem qual DMD o paciente está em uso e quando foi feita a sua última dose, pois cada fármaco pode interagir de forma diferente com a indução da resposta imune.

ABSTRACT The Scientific Department of Neuroimmunology of the Brazilian Academy of Neurology (DCNI/ABN) and Brazilian Committee for Treatment and Research in Multiple Sclerosis and Neuroimmunological Diseases (BCTRIMS) provide recommendations in this document for vaccination of the population with demyelinating diseases of the central nervous system (CNS) against infections in general and against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes COVID-19. We emphasize the seriousness of the current situation in view of the spread of COVID-19 in our country. Therefore, reference guides on vaccination for clinicians, patients, and public health authorities are particularly important to prevent some infectious diseases. The DCNI/ABN and BCTRIMS recommend that patients with CNS demyelinating diseases (e.g., MS and NMOSD) be continually monitored for updates to their vaccination schedule, especially at the beginning or before a change in treatment with a disease modifying drug (DMD). It is also important to note that vaccines are safe, and physicians should encourage their use in all patients. Clearly, special care should be taken when live attenuated viruses are involved. Finally, it is important for physicians to verify which DMD the patient is receiving and when the last dose was taken, as each drug may affect the induction of immune response differently.

https://doi.org/10.1590/0004-282x-anp-2021-0162 1 downloads

Morphometric Analyses of the Caroticoclinoid and Interclinoid Foramina: A Study in Brazilian Dry Skulls
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de-Oliveira, Kennedy Martinez

; de-Almeida, Valério Landim

; Benevenuto, Gabriel das Chagas

; de-Faria, Gustavo Maia

; Silva, Ana Clara Camillato e

; Costa, Thayná Cristina Ferreira

; de-Oliveira, Daniel Gonçalves

; Bueno, Leonardo Braga

; Rodrigues, Pedro Lucas

; Peixoto, Marcos Túlio Castro

; Gomes, Talita Cardoso

; Oliveira, Maísa de Fátima Satiro

; Gonçalves, Giuliano Roberto

; Grecco, Leandro Henrique

International Journal of Morphology

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Out 2021, Volume 39 Nº 5 Páginas 1345 - 1352

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

SUMMARY: The caroticoclinoid (CF) and the interclinoid (IF) foramina are variant formations of the middle cranial fossa, which result from idiopathic and possibly atavistic ossifications of the caroticoclinoid and interclinoid ligaments, respectively. Topographically, these foramina are intimately associated, to varying degrees, depending on their conformations, with the neurovascular elements of the paraclinoid region and have important clinical and surgical significance. Thus, this study, conducted from 38 dry skulls belonging to the shared collection of the Federal University of Juiz de Fora, Governador Valadares campus, revealed 11 skulls (28.95 %) with one of the two complete formations - the caroticoclinoid or the interclinoid, and in 6 skulls the caroticoclinoid foramen occurred uni or bilaterally. As a result, 50 % of these skulls presented the CF exclusively on the right (or 62.5 % of the total number of variant foramina), with an average transverse diameter of 4.91 mm and 5.8 mm AP (anteroposterior) diameter and an average area of 22.5 mm2. Concerning the IF, the findings occurred in 5 skulls with variable distributions between the clinoid processes and lateralities. The average length of the bone bridges in the IF was 7.7 mm. In conclusion, detailed anatomical knowledge of the caroticoclinoid and the interclinoid foramina is fundamental to the practice of neurosurgical procedures, since they have intimate relationships at a location of high structural complexity.

RESUMEN: El foramen caroticoclinoideo (FC) y el foramen interclinoideo (IC) son formaciones variables de la fosa craneal media, que resultan de osificaciones idiopáticas y posiblemente atávicas de los ligamentos caroticoclinoideo e interclinoideo, respectivamente. Estos forámenes están íntimamente asociados topográficamente en diversos grados, dependiendo de sus conformaciones, con los elementos neurovasculares de la región paraclinoidea y tienen importancia clínica y quirúrgica. Así, este estudio, realizado a partir de 38 cráneos secos pertenecientes a la colección compartida de la Universidad Federal de Juiz de Fora, campus Governador Valadares, reveló 11 cráneos (28,95 %) con una de las dos formaciones completas: el FC o el IC, y en 6 cráneos el FC se presentó uni o bilateralmente. Como resultado, el 50 % de estos cráneos presentaba el FC exclusivamente a la derecha (o el 62,5 % del total de forámenes variantes), con un diámetro transversal medio de 4,91 mm y un diámetro anteroposterior medio de 5,8 mm y un área promedio de 22,5 mm2. En cuanto a la FI, los hallazgos se presentaban en 5 cráneos con distribuciones variables entre los procesos clinoides y lateralidades. La longitud media de los puentes óseos en el FI fue de 7,7 mm. En conclusión, el conocimiento anatómico detallado de los forámenes caroticoclinoideo e interclinoideo resulta fundamental para la práctica de procedimientos neuroquirúrgicos, ya que presentan relaciones íntimas en un lugar de alta complejidad estructural.

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Management of hypopituitarism: a perspective from the Brazilian Society of Endocrinology and Metabolism
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Garmes, Heraldo Mendes

; Boguszewski, César Luiz

; Miranda, Paulo Augusto Carvalho

; Martins, Manoel Ricardo Alves

; Silva, Silvia Regina Correa da

; Abucham Filho, Julio Zaki

; Musolino, Nina Rosa de Castro

; Vilar, Lucio

; Portari, Luiz Henrique Corrêa

; Gadelha, Mônica Roberto

; Kasuki, Leandro

; Naves, Luciana Ansaneli

; Czepielewski, Mauro Antônio

; Almeida, Tobias Skrebsky de

; Duarte, Felipe Henning Gaia

; Glezer, Andrea

; Bronstein, Marcello Delano

Archives of Endocrinology and Metabolism

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Abr 2021, Volume 65 Nº 2 Páginas 212 - 230

Resumo: En | Texto: En | PDF: En

ABSTRACT Hypopituitarism is a disorder characterized by insufficient secretion of one or more pituitary hormones. New etiologies of hypopituitarism have been recently described, including head trauma, cerebral hemorrhage, and drug-induced hypophysitis. The investigation of patients with these new disorders, in addition to advances in diagnosis and treatment of hypopituitarism, has increased the prevalence of this condition. Pituitary hormone deficiencies can induce significant clinical changes with consequent increased morbidity and mortality rates, while hormone replacement based on current guidelines protects these patients. In this review, we will first discuss the different etiologies of hypopituitarism and then address one by one the clinical aspects, diagnostic evaluation, and therapeutic options for deficiencies of TSH, ACTH, gonadotropin, and GH. Finally, we will detail the hormonal interactions that occur during replacement of pituitary hormones.

https://doi.org/10.20945/2359-3997000000335 625 downloads

Clinical and molecular correlates of the ASPECTS in the acute phase of stroke
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MOURÃO, Aline Mansueto

; VICENTE, Laélia Cristina Caseiro

; ABREU, Mery Natali Silva

; SANT’ANNA, Romeu Vale

; DE MEIRA, Fidel Castro Alves

; XAVIER, Rodrigo Menezes de Brito

; TANURE, Marco Túlio de Azevedo

; VIEIRA, Erica Leandro Marciano

; DE SOUZA, Leonardo Cruz

; MIRANDA, Aline Silva de

; RACHID, Milene Alvarenga

; TEIXEIRA, Antônio Lucio

Arquivos de Neuro-Psiquiatria

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Mai 2020, Volume 78 Nº 5 Páginas 262 - 268

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Introdução: A Alberta Stroke Early Score (ASPECTS) foi desenvolvida para monitorização de alterações isquêmicas precoces na tomografia computadorizada de crânio, estando associada a desfechos clínicos. A ASPECTS também pode se associar aos biomarcadores periféricos que refletem a resposta fisiopatológica do cérebro ao AVC isquêmico. Objetivo: Investigar à associação entre os parâmetros periféricos com a Alberta Stroke Early Score (ASPECTS) em indivíduos após acidente vascular cerebral isquêmico. Métodos: Pacientes acima de 18 anos com AVC isquêmico agudo foram incluídos neste estudo. Nenhum paciente foi elegível para trombólise. Os pacientes foram submetidos à tomografia computadorizada sem contraste nas primeiras 24 horas da admissão, a ASPECTS e as avaliações clínicas e moleculares aplicadas no mesmo dia. O National Institutes of Health Stroke Scale (NIHSS), a escala de Rankin modificada e o Mini Exame do Estado Mental para avaliação clínica também foram aplicados a todos os indivíduos. Os níveis plasmáticos de BDNF, VCAM-1, VEGF, IL-1β, sTNFRs e adiponectina foram determinados por ELISA. Resultados: Pior desempenho neurológico (NIHSS), cognitivo (MEEM) e funcional (Rankin) foram observados no grupo com alterações na ASPECTS. Pacientes com alterações na ASPECTS também exibiram níveis mais altos de IL-1β e adiponectina. Na regressão multivariada linear, foi encontrado um coeficiente R ajustado de 0,515, indicando adiponectina e NIHSS como preditores independentes para a ASPECTS. Conclusão: Os níveis plasmáticos de adiponectina estão associados aos escores da ASPECTS.

ABSTRACT Background: The Alberta Stroke Program Early CT Score (ASPECTS) scale was developed for monitoring early ischemic changes on CT, being associated with clinical outcomes. The ASPECTS can also associate with peripheral biomarkers that reflect the pathophysiological response of the brain to the ischemic stroke. Objective: To investigate the association between peripheral biomarkers with the Alberta Stroke Program Early CT Score (ASPECTS) in individuals after ischemic stroke. Methods: Patients over 18 years old with acute ischemic stroke were enrolled in this study. No patient was eligible for thrombolysis. The patients were submitted to non-contrast CT in the first 24 hours of admission, being the Alberta Stroke Program Early CT Score and clinical and molecular evaluations applied on the same day. The National Institutes of Health Stroke Scale (NIHSS), modified Rankin scale and the Mini-Mental State Examination for clinical evaluation were also applied to all subjects. Plasma levels of BDNF, VCAM-1, VEGF, IL-1β, sTNFRs and adiponectin were determined by ELISA. Results: Worse neurological impairment (NIHSS), cognitive (MEEM) and functional (Rankin) performance was observed in the group with changes in the NCTT. Patients with NCTT changes also exhibited higher levels of IL-1β and adiponectin. In the linear multivariate regression, an adjusted R coefficient of 0.515 was found, indicating adiponectin and NIHSS as independent predictors of ASPECTS. Conclusion: Plasma levels of adiponectin are associated with the ASPECTS scores.

https://doi.org/10.1590/0004-282x20200001 1343 downloads

10.

Soil quality and soybean productivity in crop-livestock integrated system in no-tillage
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Laroca, Jackeline Vieira dos Santos

; Souza, Juliana Mendes Andrade de

; Pires, Gabriela Castro

; Pires, Gleidson José Coutinho

; Pacheco, Leandro Pereira

; Silva, Francine Damian da

; Wruck, Flávio Jesus

; Carneiro, Marco Aurélio Carbone

; Silva, Laércio Santos

; Souza, Edicarlos Damacena de

Pesquisa Agropecuária Brasileira

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Nov 2018, Volume 53 Nº 11 Páginas 1248 - 1258

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo: O objetivo deste trabalho foi avaliar a qualidade do solo e sua relação com a produtividade de soja (Glycine max) em sistema de integração lavoura-pecuária (ILP), com consórcio entre gramíneas e leguminosas na fase pastagem. O experimento foi realizado no Estado de Mato Grosso, em um Latossolo Vermelho distrófico, em que gramíneas (Megathyrsus maximus 'BRS Tamani' e Urochloa brizantha 'BRS Piatã'), consorciadas com feijão-caupi (Vigna unguiculata 'BRS Tumucumaque') e feijão-guandu (Cajanus cajan 'BRS Mandarim'), foram cultivadas após a colheita da soja. Um delineamento experimental de blocos ao acaso foi utilizado com três repetições, em arranjo de parcelas subdivididas, em que as gramíneas foram consideradas como parcelas, e as leguminosas, como subparcelas. Os consórcios com leguminosas proporcionaram incrementos dos estoques de C e N total. Os consórcios causaram o aumento de C e N da biomassa microbiana, enquanto os cultivos solteiros contribuíram para o estresse da microbiota do solo. A atividade da enzima urease foi sensível às alterações de manejo em curto prazo, porém a fosfatase ácida e a β-glucosidase foram indicadores pouco sensíveis. A qualidade do solo é elevada com os consórcios entre gramíneas e leguminosas, com efeitos positivos sobre a produtividade de grãos de soja.

Abstract: The objective of this work was to evaluate the quality of the soil and its relation with soybean (Glycine max) yield in an integrated crop-livestock system (ICLS), with intercropping between grasses and legumes in the pasture phase. The experiment was carried out in the state of Mato Grosso, Brazil, on a dystrophic Oxisol, in which grasses (Megathyrsus maximus 'BRS Tamani' and Urochloa brizantha 'BRS Piatã'), intercropped with cowpea (Vigna unguiculata 'BRS Tumucumaque') and pigeon pea (Cajanus cajan 'BRS Mandarim'), were cultivated after soybean harvest. A randomized complete block design was used, with three replicates, in a split-plot arrangement, in which grasses were considered as plots, and legumes as subplots. Legume intercrops provided increases of C and total N stocks. The intercrops caused the increase of C and N of the microbial biomass, whereas the single cultures contributed to stress in the soil microbiota. The activity of the urease enzyme was sensitive to management changes in the short term, but acid phosphatase and β-glucosidase were poorly sensitive indicators. Soil quality is high with intercropping between grasses and legumes, with positive effects on soybean grain yield.

https://doi.org/10.1590/s0100-204x2018001100007 1725 downloads

11.

Potassium-modulated physiological performance of mango plants infected by Ceratocystis fimbriata
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Cacique, Isaias Severino

; Bispo, Wilka Messner Silva

; Araujo, Leonardo

; Aucique-Pérez, Carlos Eduardo

; Rios, Jonas Alberto

; Silva, Leandro Castro

; Rodrigues, Fabrício Ávila

Bragantia

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Dez 2017, Volume 76 Nº 4 Páginas 521 - 535

Resumo: En | Texto: En | PDF: En

ABSTRACT Mango wilt, caused by the fungus Ceratocystis fimbriata, is an important disease affecting mango production. In view of the beneficial effects of potassium (K) in other profitable crops and the lack of information about the effect of macronutrients on mango wilt development, the present study aimed to evaluate how mango plants supplied with K respond physiologically when infected by C. fimbriata. Mango plants (» 3 years old) from cultivar Ubá were grown in plastic pots containing 58 mg of K·dm−3 (original K level based on the chemical analysis of the substrate) or in plastic pots with substrate amended with a solution of 0.5 M potassium chloride (KCl) to achieve the rate of 240 mg K·dm−3. Disease symptoms were more pronounced in inoculated plants grown at the lower K level. Substantial declines in stomatal conductance, in line with decreases in the internal-to-ambient CO2 concentration ratio and the absence of detectable changes in the chlorophyll a fluorescence parameters, suggest that the decrease in the net carbon assimilation rate is due, at least initially, to stomatal limitations. High concentrations of K and manganese were found in the stem tissues of inoculated plants and supplied with the highest K rate, most likely due to the involvement of these tissues in the local development of defense mechanisms. The results of this study suggest that the supply of K favored the physiological performance of mango plants and their resistance against C. fimbriata infection.

https://doi.org/10.1590/1678-4499.2016.264 1339 downloads

12.

Anti-inflammatory potential of microemulsion and pure bullfrog oil in muscle injury
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Davim, André Luiz Silva

; Dantas, Tereza Neuma de Castro

; Albuquerque, Diego Filgueira

; Pereira, Márcia Rodrigues

; Queiroz, Laíse Beatriz Trindade da Silva

; Freitas, Leandro Moura de

Revista Brasileira de Medicina do Esporte

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Mai 2017, Volume 23 Nº 3 Páginas 237 - 240

Resumo: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Introdução: Todos os dias, a ciência busca novas formas de tratar diversas doenças por meio de fármacos que sejam eficientes e viáveis. Desse modo, as alternativas terapêuticas que atendam tal demanda são alvos de estudo. As microemulsões são uma dessas novas alternativas, em razão de suas características farmacodinâmicas e farmacocinéticas peculiares. Objetivo: O objetivo deste estudo foi analisar o potencial anti-inflamatório do óleo de rã-touro puro e em microemulsão usando um modelo experimental de lesão muscular. Métodos: Foram utilizados camundongos machos da linhagem Swiss, divididos em três grupos: controle, microemulsão e óleo de rã-touro puro. Após o pré-tratamento, induziu-se uma lesão muscular na perna dos animais e, em seguida, foram realizadas avaliações da extensão horizontal do edema e comparadas entre os grupos em tempos predeterminados. Depois da avaliação da lesão muscular, realizou-se a dissecção dos músculos gastrocnêmios direitos para análise histológica. Resultados: A microemulsão e o óleo de rã-touro puro apresentaram boa atividade anti-inflamatória, atuando de forma semelhante na redução do edema durante as primeiras duas horas, porém sem significância estatística da 3ª até a 24ª hora depois da indução. Nas análises histológicas, observou-se que no tecido muscular dos animais tratados com a microemulsão houve discreta presença de infiltrado celular e pouco desgaste das fibras musculares quando comparado com o tecido muscular dos animais tratados com o óleo de rã-touro puro. A análise histológica do tecido hepático mostrou sinais de lesão nos lobos hepáticos dos animais do grupo óleo de rã-touro puro, não observada nos animais do grupo microemulsão. Conclusão: A microemulsão apresentou bom potencial anti-inflamatório na fase aguda da resposta inflamatória, atenuando a formação de edema e preservando o tecido muscular contra a ocorrência de lesões e sem indução de lesão no tecido hepático.

ABSTRACT Introduction: Every day science seeks new ways to treat various diseases through drugs that are efficient and viable. Thus, therapeutic alternatives that meet such demand are targets of study. Microemulsions are one of these new alternatives due to their peculiar pharmacodynamic and pharmacokinetic characteristics. Objective: The aim of this study was to analyze the anti-inflammatory potential of microemulsion and pure bullfrog oil using an experimental model of muscle injury. Methods: Male Swiss mice were divided into three groups: control, microemulsion and pure bullfrog oil. After the pre-treatment, a muscle injury was induced in the animals’ leg and subsequently evaluations were carried out in the horizontal extent of edema and compared between the groups at predetermined times. Following evaluation of muscle injury, dissection of the right gastrocnemius muscles was performed for histological analysis. Results: The microemulsion and pure bullfrog oil showed good anti-inflammatory activity, acting similarly in reducing edema during the first two hours, but without statistical significance from the 3rd to the 24th hour after induction. The histological analysis revealed that the muscle tissue of the animals treated with the microemulsion presented mild cellular infiltrate and little wear of muscle fibers when compared with the muscular tissue of animals treated with the pure bullfrog oil. The histological analysis of the hepatic tissue showed signs of injury in the liver lobes of the pure bullfrog oil group, not observed in the microemulsion group. Conclusion: The microemulsion sho-wed good anti-inflammatory potential in the acute phase of the inflammatory response, reducing the formation of edema and preserving muscle tissue against the occurrence of lesions and without inducing injury in hepatic tissue.

RESUMEN Introducción: Todos los días, la ciencia busca nuevas formas para tratar diversas enfermedades por medio de fármacos eficientes y viables. Así, las alternativas terapéuticas que puedan atender esta demanda son objeto de estudio. Las microemulsio-nes son una de estas nuevas alternativas debido a sus características farmacodinámicas y farmacocinéticas únicas. Objetivo: El objetivo de este estudio fue analizar el potencial antiinflamatorio de la microemulsión y el aceite puro de la rana toro utilizando un modelo experimental de lesión muscular. Métodos: Fueron utilizados ratones machos de la cepa Swiss, divididos en tres grupos: control, microemulsión y aceite puro de la rana toro. Después del pretratamiento, se ha creado una lesión muscular en las piernas de los animales y luego se realizaron evaluaciones de la extensión horizontal del edema y comparadas entre grupos en momentos predeterminados. Después de la evaluación de la lesión muscular, se llevó a cabo la disección de los músculos gastrocnemios derechos para el análisis histológico. Resultados: La microemulsión y el aceite de rana toro puro mostraron buena actividad antiinflamatoria, actuando de manera similar para reducir el edema durante las primeras dos horas, pero sin significación estadística desde la tercera hasta la 24.a hora después de la inducción. Los análisis histológicos revelaron que el tejido muscular de los animales tratados con microemulsión presentó presencia moderada de infiltrado celular y poco desgaste de las fibras musculares en comparación con el tejido muscular de los animales tratados con el aceite de rana puro. El análisis histológico del tejido hepático mostró señales de lesión en los lóbulos del hígado de los animales del grupo aceite puro de rana toro, que no fueron observadas en los animales del grupo microemulsión. Conclusión: La microemulsión mostró un buen potencial antiinflamatorio en la fase aguda de la respuesta inflamatoria, la atenuación de la formación de edema y la preservación del tejido muscular en contra de la aparición de lesiones y sin inducción de lesión en el tejido hepático.

https://doi.org/10.1590/1517-869220172303159519 1586 downloads

13.

Differential leaf gas exchange performance of mango cultivars infected by different isolates of Ceratocystis fimbriata
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Bispo, Wilka Messner da Silva

; Araujo, Leonardo

; Moreira, Wiler Ribas

; Silva, Leandro de Castro

; Rodrigues, Fabrício Ávila

Scientia Agricola

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Abr 2016, Volume 73 Nº 2 Páginas 150 - 158

Resumo: En | Texto: En | PDF: En

ABSTRACT Caused by the vascular fungus Ceratocystis fimbriata, mango wilt is considered to be one of the most serious threats in mango-producing regions worldwide. However, changes in leaf gas exchange level and the mechanisms underlying host responses to this fungal infection remain poorly described. This study aimed to evaluate potential changes in the leaf gas exchange of different mango cultivars (Ubá, Espada, Haden and Tommy Atkins) in response to two Brazilian isolates of C. fimbriata (CEBS15 and MSAK16) to non-invasively assess cultivar variability in relation to the basal level of resistance to mango wilt. Both isolates, regardless of the cultivar, caused reductions in stomatal conductance and, thus, a reduction in CO2 assimilation via diffusive limitations. Taking into account the full length of the internal lesion and the radial colonization of the stem tissues, both isolates showed equivalent aggressiveness when inoculated into the Haden and Tommy Atkins cultivars. Conversely, when compared to the CEBS15 isolate of C. fimbriata, the MSAK16 isolate was more aggressive in cv. Espada and less aggressive in cv. Ubá.

https://doi.org/10.1590/0103-9016-2015-0022 1437 downloads

14.

BURNOUT SYNDROME IN YOUNG FEMALE VOLLEYBALL ATHLETES PARTICIPANTS OF THE BRAZILIAN CHAMPIONSHIP TEAMS
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Teixeira, Marcelo de Castro

; Batista, Marco Túlio Silva

; Leite, Leandro Rafael

; Silva, Sandro Fernandes Da

Journal of Physical Education

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2016, Volume 27 elocation e2717

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O Voleibol é um dos principais esportes do Brasil e para atingir bons níveis competitivos são necessários treinamentos com altos volume e intensidade, que consequentemente geram desgastes físicos e psicológicos. O resultado deste processo pode ocasionar um estresse crônico nos praticantes, conhecido como Síndrome de Burnout. O presente estudo objetivou investigar a ocorrência desta síndrome em atletas de Voleibol do sexo feminino participantes dos Campeonatos Brasileiros de Seleções no ano de 2013. Foi aplicado o Questionário de Burnout para Atletas, respondido por 576 jogadoras (M=16.4±1.12 anos). Utilizou-se de estatística descritiva para caracterização da síndrome e o Teste de Correlação Não Paramétrico de Kendall's tau-b para verificação da correlação entre as dimensões do burnout e o Tempo de Prática (TP). As atletas apresentaram escores médios de 2.01 ±0.53 para burnout total e correlações significativas com TP de sete anos ou mais, além do TP Geral.

ABSTRACT Volleyball is one of the leading sports in Brazil and, in order to achieve good competitive levels, many training sessions are required which consequently generates physical and psychological wear. The result of this process can lead to chronic stress in practitioners, known as the Burnout Syndrome. This study aimed to investigate the occurrence of this syndrome in volleyball female athletes participating on the Brazilian Regional Teams Championship 2013. The Athlete burnout Questionnaire was answered by 576 players (M = 16.4years, SD = 1.12). We used descriptive statistics of mean and standard deviation to characterize the syndrome and the non-parametric correlation test of Kendall's tau-b to verify the correlation between the dimensions of burnout and the Practice Time (PT). The athletes presented mean scores (2.01±0:53) for complete burnout and significant correlations with TP of seven years or more in addition to the General.

https://doi.org/10.4025/jphyseduc.v27i1.2717 2320 downloads

15.

Mecanismos bioquímicos da defesa do algodoeiro à mancha de ramulária mediados pelo silício
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Curvêlo, Carmen Rosa da Silva

; Rodrigues, Fabrício Ávila

; Silva, Leandro de Castro

; Nascimento, Kelly Juliane Telles

; Berger, Paulo Geraldo

Bragantia

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Mar 2013, Volume 72 Nº 1 Páginas 41 - 51

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Esse estudo investigou o efeito do silício (Si) na resistência do algodoeiro à mancha de ramulária (Ramularia areola). Plantas de algodoeiro (cvs. NuOpal e BRS Buriti) foram cultivadas em solução nutritiva nas concentrações de 0 (-Si) e 2 mM Si L-1 (+Si) e aos 30 dias após emergência foram submetidas à inoculação com uma suspensão de conídios de R. areola. Avaliou-se o período de incubação (PI), o período latente (PL60), a severidade, o número de lesões (NL) por cm² de folha, o tamanho de lesão (TL), a concentração foliar de Si e as atividades das enzimas de defesa peroxidases (POX), polifenoloxidases (PFO), quitinases (QUI), β-1,3-glucanases (GLU) e fenilalanina amônia-liases (FAL). Os dados de severidade foram usados para calcular a área abaixo da curva do progresso da mancha de ramulária (AACPMR). A concentração foliar de Si aumentou em 64% nas plantas supridas com Si em relação às não supridas com esse elemento. Houve aumentos de 10% e 14,7%, respectivamente, para o PI e o PL60 nas plantas supridas com Si. Reduções de 38,6% e 62,4% para o NL e de 17,2% e 26,6% para o TL ocorrem, respectivamente, nas plantas das cvs. NuOpal e BRS Buriti supridas com Si. Houve redução de 35% na AACPMR para as plantas supridas com Si em relação às não supridas. A concentração de compostos fenólicos solúveis totais nas plantas das duas cultivares supridas com Si foi maior aos 21 dias após inoculação (dai) em relação às não supridas com esse elemento. A concentração dos derivados da lignina foi maior dos 15 aos 21 dai apenas para as plantas da cv. BRS Buriti supridas com Si. A atividade da POX foi maior nas plantas das duas cultivares supridas com Si em relação às não supridas com esse elemento dos 15 aos 21 dai. Paras as plantas da cv. NuOpal supridas com Si, as atividades da PFO, QUI, GLU e FAL aumentaram aos 18 dai. As atividades da PFO e da FAL nas plantas da cv. BRS Buriti não foram potencializadas pelo Si. Nas plantas da cv. BRS Buriti supridas com Si, houve aumento nas atividades da QUI e da GLU aos 21 dai em relação às não supridas com Si. Conclui-se que a resistência do algodoeiro à mancha de ramulária foi bioquimicamente potencializada pelo Si, principalmente para as plantas da cv. NuOpal consideradas suscetíveis à mancha de ramulária.

This study investigated the effect of silicon (Si) on cotton resistance to ramularia leaf spot (Ramularia areola). Plants of cotton (cvs. NuOpal and BRS Buriti) were grown in nutrient solution containing 0 (+Si) or 2 mM Si L-1 (-Si) and inoculated with a conidial suspension of R. areola at 30 days after emergence. The incubation period (IP), latent period (LP60), severity, number of lesions (NL) per cm² of leaf area, lesion size (LS), foliar Si concentration and the activities of defense enzymes peroxidases (POX), polyphenoloxidases (PPO), chitinases (CHI), β-1,3-glucanases (GLU), and phenylalanine ammonia-lyases (PAL) were evaluated. Data from severity were used to calculate the area under ramularia leaf spot progress curve (AURLSPC). Leaf Si concentration increased by 64% on plants supplied with Si compared to plants not supplied with this element. There were increases of 10 and 14.7% for IP and LP60, respectively, on plants supplied with Si. Reductions of 38.6 and 62.4% for NL and 17.2 and 26.6% for LS occurred, respectively, for plants from NuOpal and BRS Buriti cvs supplied with Si. AURLSPC was reduced by 35% for the +Si treatment compared to the -Si treatment. The concentration of total soluble phenolic compounds on plants of both cv. supplied with Si increased during the progress of ramularia, but the lowest values occurred for the -Si treatment until 18 days after inoculation (dai). The increase on the concentration of lignin derivatives was significant only for plants of cv. BRS Buriti infected by R. areola and supplied with Si. POX activity was higher on plants from the two cultivars supplied with Si compared to plants not supplied with this element. For plants of cv. NuOpal supplied with Si, PPO, CHI, GLU, and PAL activities increased until 18 dai, but the activities of PAL and PPO on plants of cv. BRS Buriti were not potentiated by Si. On plants from cv. BRS Buriti supplied with Si, there was increase on CHI and GLU activities at 21 dai compared to plants not supplied with Si. It can be concluded that cotton resistance to ramularia leaf spot was biochemically enhanced by Si, especially for plants of cv. NuOpal considered susceptible to ramularia leaf spot.

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