Pesquisa | SciELO

Abstract Objective: To assess factors associated with emotional changes and Hyperactivity/Inattention (HI) motivated by COVID-19 quarantine in adolescents with immunocompromising diseases. Methods: A cross-sectional study included 343 adolescents with immunocompromising diseases and 108 healthy adolescents. Online questionnaires were answered including socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and validated surveys: Strengths and Difficulties Questionnaire (SDQ), Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0). Results: The frequencies of abnormal emotional SDQ scores from adolescents with chronic diseases were similar to those of healthy subjects (110/343 [32%] vs. 38/108 [35%], p = 0.548), as well as abnormal hyperactivity/inattention SDQ scores (79/343 [23%] vs. 29/108 [27%], p = 0.417). Logistic regression analysis of independent variables associated with abnormal emotional scores from adolescents with chronic diseases showed: female sex (Odds Ratio [OR = 3.76]; 95% Confidence Interval (95% CI) 2.00–7.05; p < 0.001), poor sleep quality (OR = 2.05; 95% CI 1.08–3.88; p = 0.028) and intrafamilial violence during pandemic (OR = 2.17; 95% CI 1.12–4.19; p = 0.021) as independently associated with abnormal emotional scores, whereas total PedsQL score was inversely associated with abnormal emotional scores (OR = 0.95; 95% CI 0.93–0.96; p < 0.0001). Logistic regression analysis associated with abnormal HI scores from patients evidenced that total PedsQL score (OR = 0.97; 95% CI 0.95–0.99; p = 0.010], changes in medical appointments during the pandemic (OR = 0.39; 95% CI 0.19–0.79; p = 0.021), and reliable COVID-19 information (OR = 0.35; 95% CI 0.16–0.77; p = 0.026) remained inversely associated with abnormal HI scores. Conclusion: The present study showed emotional and HI disturbances in adolescents with chronic immunosuppressive diseases during the COVID-19 pandemic. It reinforces the need to promptly implement a longitudinal program to protect the mental health of adolescents with and without chronic illnesses during future pandemics. Objective HyperactivityInattention Hyperactivity Inattention (HI COVID19 COVID 19 COVID-1 Methods crosssectional cross sectional 34 10 sociodemographic socio demographic selfrated self rated surveys SDQ, , (SDQ) PSQI, PSQI (PSQI) 40 4 0 4. PedsQL4.0. PedsQL40 PedsQL4.0 . PedsQL4 (PedsQL4.0) Results 110/343 110343 110 (110/34 32% 32 [32% vs 38108 38 38/10 35%, 35 35% [35%] 0.548, 0548 0.548 548 0.548) hyperactivityinattention hyperactivity inattention 79/343 79343 79 (79/34 23% 23 [23% 29108 29 29/10 27%, 27 27% [27%] 0.417. 0417 0.417 417 0.417) Odds OR 3.76 376 3 76 3.76] 95 (95 2.00–7.05 200705 2 00 7 05 0.001, 0001 0.001 001 0.001) 2.05 205 1.08–3.88 108388 1 08 88 0.028 0028 028 2.17 217 17 1.12–4.19 112419 12 0.021 0021 021 0.95 095 0.93–0.96 093096 93 96 0.0001. 00001 0.0001 0.0001) 0.97 097 97 0.95–0.99 095099 99 0.010, 0010 0.010 010 0.010] 0.39 039 39 0.19–0.79 019079 0.021, 0.35 035 0.16–0.77 016077 16 77 0.026 0026 026 Conclusion pandemics COVID1 COVID- (SDQ (PSQI PedsQL4. (PedsQL4.0 110/34 11034 11 (110/3 [32 3810 38/1 [35% 054 0.54 54 79/34 7934 (79/3 [23 2910 29/1 [27% 041 0.41 41 3.7 37 9 (9 2.00–7.0 20070 000 0.00 2.0 20 1.08–3.8 10838 8 0.02 002 02 2.1 21 1.12–4.1 11241 0.9 09 0.93–0.9 09309 0000 0.000 0.95–0.9 09509 0.01 01 0.3 03 0.19–0.7 01907 0.16–0.7 01607 (PedsQL4. 110/3 1103 (110/ [3 381 38/ [35 0.5 5 79/3 793 (79/ [2 291 29/ [27 04 0.4 3. ( 2.00–7. 2007 0.0 2. 1.08–3. 1083 1.12–4. 1124 0. 0.93–0. 0930 0.95–0. 0950 0.19–0. 0190 0.16–0. 0160 (PedsQL4 110/ (110 [ 79/ (79 2.00–7 200 1.08–3 1.12–4 112 0.93–0 093 0.95–0 0.19–0 019 0.16–0 016 (PedsQL (11 (7 2.00– 1.08– 1.12– 0.93– 0.95– 0.19– 0.16– (1 2.00 1.08 1.12 0.93 0.19 0.16 1.0 1.1 0.1 1.

https://doi.org/10.1016/j.clinsp.2023.100167

Electrical and Optical Properties of Poly(vinyl chloride)/ZnS Nanocomposites Exposed to Gamma Radiation Polyvinyl Poly vinyl chloride/ZnS chlorideZnS chloride /ZnS ZnS
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Guimarães, Nara E.

; Ximenes, Érika R. B. Silva, Lindomar Avelino da Santos, Renata Francisca da Silva Araújo, Elmo Silvano Aquino, Kátia Aparecida da Silva

Materials Research

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2023, Volume 26 elocation e20220308

Resumo: En | Texto: En | PDF: En

Poly(vinyl chloride) (PVC) is considered one of the most versatile polymers due to its interaction with different additives. On the other hand, binary sulfides have wide applications as zinc sulfide (ZnS), one of the first discovered semiconductors. This study aims to synthesize a nanocomposite material containing nano ZnS in the PVC matrix with a surfactant as a compatibilizer agent to evaluate its optical and electrical properties when exposed to gamma radiation. Our results showed that adding ZnS at 0.5 wt% concentration in the PVC matrix promoted the system from insulating to the semiconductor material. Therefore, gamma radiation has played an important role in nanocomposites' optical and electrical properties with changes in electrical and electrical conductivity, constant dielectric parts, refraction index, optical band gap, and Urbach energy. Thus, our study points to the development of nanocomposite material, semiconductors, and features useful for applications in flexible electronics or optical-electronic devices. Polyvinyl Poly vinyl chloride (PVC additives hand ZnS, , (ZnS) semiconductors 05 0 5 0. wt Therefore nanocomposites conductivity parts index gap energy Thus opticalelectronic electronic devices (ZnS

https://doi.org/10.1590/1980-5373-mr-2022-0308

Prediction of intensive care admission and hospital mortality in COVID-19 patients using demographics and baseline laboratory data COVID19 COVID 19 COVID-1 COVID1 1 COVID-
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Avelino-Silva, Vivian I.

; Avelino-Silva, Thiago J.

; Aliberti, Marlon J.R.

; Ferreira, Juliana C.

; Cobello Junior, Vilson

; Silva, Katia R.

; Pompeu, Jose E. Antonangelo, Leila Magri, Marcello M. Barros Filho, Tarcisio E.P. Souza, Heraldo P. Kallás, Esper G.

Clinics

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2023, Volume 78 elocation 100183

Resumo: En | Texto: En | PDF: En

Abstract Introduction: Optimized allocation of medical resources to patients with COVID-19 has been a critical concern since the onset of the pandemic. Methods: In this retrospective cohort study, the authors used data from a Brazilian tertiary university hospital to explore predictors of Intensive Care Unit (ICU) admission and hospital mortality in patients admitted for COVID-19. Our primary aim was to create and validate prediction scores for use in hospitals and emergency departments to aid clinical decisions and resource allocation. Results: The study cohort included 3,022 participants, of whom 2,485 were admitted to the ICU; 1968 survived, and 1054 died in the hospital. From the complete cohort, 1,496 patients were randomly assigned to the derivation sample and 1,526 to the validation sample. The final scores included age, comorbidities, and baseline laboratory data. The areas under the receiver operating characteristic curves were very similar for the derivation and validation samples. Scores for ICU admission had a 75% accuracy in the validation sample, whereas scores for death had a 77% accuracy in the validation sample. The authors found that including baseline flu-like symptoms in the scores added no significant benefit to their accuracy. Furthermore, our scores were more accurate than the previously published NEWS-2 and 4C Mortality Scores. Discussion and conclusions: The authors developed and validated prognostic scores that use readily available clinical and laboratory information to predict ICU admission and mortality in COVID-19. These scores can become valuable tools to support clinical decisions and improve the allocation of limited health resources. Introduction COVID19 COVID 19 COVID-1 pandemic Methods (ICU COVID19. 19. Results 3022 3 022 3,02 participants 2485 2 485 2,48 196 survived 105 1496 1 496 1,49 1526 526 1,52 age comorbidities samples 75 77 flulike flu like Furthermore NEWS2 NEWS NEWS- C conclusions COVID1 COVID- 302 02 3,0 248 48 2,4 10 149 49 1,4 152 52 1,5 7 30 0 3, 24 4 2, 14 1, 15 5

https://doi.org/10.1016/j.clinsp.2023.100183

MOLECULAR NETWORKING-BASED DEREPLICATION OF AMBUIC ACID DERIVATIVES FROM THE MARINE FUNGUS PESTALOTIOPSIS SP. 4A11
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Kellner Filho, Luis C.

; Assis, Rhenner N. A. Santos, Aline O. dos Targanski, Sabrina K. Siqueira, Kátia A. Medeiros, Livia S. de Angolini, Célio F. F. Silva, Felipe M. A. da Soares, Marcos A. Dias, Gustavo M. Silva, Marcio L. A. Cunha, Wilson R. Pauletti, Patricia M. Koolen, Hector H. F. Januario, Ana H.

Química Nova

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2022, Volume 45 Nº 3 Páginas 268 - 274

Resumo: En | Texto: En | PDF: En

Ambuic acid (AA) is a highly-modified cyclohexenone and known as a promising inhibitor of quorum sensing in methicillin-resistant Staphylococcus aureus, and is thus a candidate as an antivirulence drug. This molecule is mainly produced by the species of Pestalotiopsis and, since its discovery twenty years ago, only a restricted amount of AA-derivatives have been described. Despite being a promising subject, methods for the analysis of modified AA-analogues via mass spectrometry remain unexplored. In order to adress this question, the marine fungus Pestalotiopsis sp. 4A11 associated with the ascidian Didemnum perlucidum was grown in a solid rice medium and its crude extract was chemically studied. From this extract, AA and 10-hydroxy ambuic acid (10-HAA) were isolated and identified using NMR spectroscopy with the aim of obtaining model compounds for the MS analysis. These served as reference compounds (seeds) to guide the dereplication of other AA-analogues via LC-MS/MS-based molecular networking. Based on the manual interpretation of the fragmentation pathways of the seeds and related compounds observed in the networks, six AA-derivatives were dereplicated in the extract. Furthermore, three analogues with unprecedented chemical formulas were proposed as putative unprecedented AA-derivatives. The fragmentation annotation proposed represents a fast and feasible method for characterizing AA-derivatives.

https://doi.org/10.21577/0100-4042.20170819

INCORPORAÇÃO DE RODAMINA B EM MEMBRANA DE POLIAMIDA PELO MÉTODO DA ADSORÇÃO
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Sousa, Kalil Z. R. de

; Faria, Emerson H. de Marçal, Liziane Ciuffi, Katia J. Ricci, Emiliane G. Rocha, Lucas A. Nassar, Eduardo J. Silva, Jorge V. L. Oliveira, Marcelo F. Maia, Izaque A.

Química Nova

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Fev 2021, Volume 44 Nº 2 Páginas 154 - 160

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

We have incorporated rhodamine into polyamide membranes by additive manufacture selective laser sintering technology. Incorporation into 0.5 cm2 membranes was conducted at different pH values and constant temperature of 25 oC. The amounts of incorporated dye were determined by a calibration curve, and analyses showed rhodamine incorporation in the order of 10-6 mol L-1. The absorption spectra of the solutions and membranes after rhodamine incorporation showed maximum absorption at 553 nm and a shoulder at 522 nm, which indicated that a dimer was formed. Photoluminescence presented maximum emission at 575 nm for the sample incorporated with rhodamine in acid pH. The membranes that were treated with acid presented shorter adsorption time and demanded 180 min to reach equilibrium, whereas the non-treated membrane and the membranes that were treated with base required 1440 min to reach equilibrium. Polyamide membrane has proven to be an excellent material for the incorporation of dyes and compounds for application as luminescent sensors.

https://doi.org/10.21577/0100-4042.20170670 134 downloads

New distributional records of species of the family Pseudorhombilidae Alcock, 1900 (Crustacea: Decapoda) from Brazilian waters
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Almeida Alves-Júnior, Flavio de

; Viana, Girlene Fábia Segundo

; Torres, Maria Fernanda Abrantes

; Silva, Kátia Cristina de Araújo

; Cintra, Israel Hidenburgo Aniceto

Nauplius

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2021, Volume 29 elocation e2021016

Resumo: En | Texto: En | PDF: En

Abstract The family Pseudorhombilidae Alcock, 1900 is widely distributed in the tropical western Atlantic, occurring from shallow waters to the continental slope, inhabiting sandy, gravelly, or muddy substrates. Despite the broad distribution of this family in the Americas, the species Pseudorhombila octodentata Rathbun, 1906 and Pseudorhombila quadridentata (Latreille, 1828) have been observed only in a few localities, with disjunct records, and Robertsella meridionalis Tavares and Gouvêa, 2013 has been recorded only in one type locality (Santa Catarina, Brazil). The present work reports new occurrences of P. octodentata from the state of Sergipe and P. quadridentata from the state of Amapá, and the second occurrence of R. meridionalis from the state of Rio Grande do Norte, Brazil. These new records are an important contribution to the understanding of distributional patterns of pseudorhombilid crab species in Brazil and to the increase in biodiversity data of crustaceans from the North and Northeast regions of Brazil.

https://doi.org/10.1590/2358-2936e2021016 189 downloads

Persistent symptoms and decreased health-related quality of life after symptomatic pediatric COVID-19: A prospective study in a Latin American tertiary hospital
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Fink, Thais T.

; Marques, Heloisa H.S.

; Gualano, Bruno

; Lindoso, Livia

; Bain, Vera

; Astley, Camilla

; Martins, Fernanda

; Matheus, Denise

; Matsuo, Olivia M.

; Suguita, Priscila

; Trindade, Vitor

; Paula, Camila S.Y.

; Farhat, Sylvia C.L.

; Palmeira, Patricia

; Leal, Gabriela N.

; Suzuki, Lisa

; Odone Filho, Vicente

; Carneiro-Sampaio, Magda

; Duarte, Alberto José S.

; Antonangelo, Leila

; Batisttella, Linamara R.

; Polanczyk, Guilherme V.

; Pereira, Rosa Maria R.

; Carvalho, Carlos Roberto R.

; Buchpiguel, Carlos A.

; Latronico, Ana Claudia

; Seelaender, Marilia

; Silva, Clovis Artur

; Pereira, Maria Fernanda B.

; Sallum, Adriana M. E. Brentani, Alexandra V. M. Neto, Álvaro José S. Ihara, Amanda Santos, Andrea R. Canton, Ana Pinheiro M. Watanabe, Andreia Santos, Angélica C. dos Pastorino, Antonio C. Franco, Bernadette D. G. M. Caruzo, Bruna Ceneviva, Carina Martins, Carolina C. M. F. Prado, Danilo Abellan, Deipara M. Benatti, Fabiana B. Smaria, Fabiana Gonçalves, Fernanda T. Penteado, Fernando D. Castro, Gabriela S. F. de Gonçalves, Guilherme S. Roschel, Hamilton Disi, Ilana R. Marques, Isabela G. Castro, Inar A. Buscatti, Izabel M. Faiad, Jaline Z. Fiamoncini, Jarlei Rodrigues, Joaquim C. Carneiro, Jorge D. A. Paz, Jose A. Ferreira, Juliana C. Ferreira, Juliana C. O. Silva, Katia R. Bastos, Karina L. M. Kozu, Katia Cristofani, Lilian M. Souza, Lucas V. B. Campos, Lucia M. A. Silva Filho, Luiz Vicente R. F. Sapienza, Marcelo T. Lima, Marcos S. Garanito, Marlene P. Santos, Márcia F. A. Dorna, Mayra B. Aikawa, Nadia E. Litvinov, Nadia Sakita, Neusa K. Gaiolla, Paula V. V. Pasqualucci, Paula Toma, Ricardo K. Correa-Silva, Simone Sieczkowska, Sofia M. Imamura, Marta Forsait, Silvana Santos, Vera A. Zheng, Yingying

Clinics

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2021, Volume 76 elocation e3511

Resumo: En | Texto: En | PDF: En

OBJECTIVES: To prospectively evaluate demographic, anthropometric and health-related quality of life (HRQoL) in pediatric patients with laboratory-confirmed coronavirus disease 2019 (COVID-19) METHODS: This was a longitudinal observational study of surviving pediatric post-COVID-19 patients (n=53) and pediatric subjects without laboratory-confirmed COVID-19 included as controls (n=52) was performed. RESULTS: The median duration between COVID-19 diagnosis (n=53) and follow-up was 4.4 months (0.8-10.7). Twenty-three of 53 (43%) patients reported at least one persistent symptom at the longitudinal follow-up visit and 12/53 (23%) had long COVID-19, with at least one symptom lasting for >12 weeks. The most frequently reported symptoms at the longitudinal follow-up visit were headache (19%), severe recurrent headache (9%), tiredness (9%), dyspnea (8%), and concentration difficulty (4%). At the longitudinal follow-up visit, the frequencies of anemia (11% versus 0%, p=0.030), lymphopenia (42% versus 18%, p=0.020), C-reactive protein level of >30 mg/L (35% versus 0%, p=0.0001), and D-dimer level of >1000 ng/mL (43% versus 6%, p=0.0004) significantly reduced compared with baseline values. Chest X-ray abnormalities (11% versus 2%, p=0.178) and cardiac alterations on echocardiogram (33% versus 22%, p=0.462) were similar at both visits. Comparison of characteristic data between patients with COVID-19 at the longitudinal follow-up visit and controls showed similar age (p=0.962), proportion of male sex (p=0.907), ethnicity (p=0.566), family minimum monthly wage (p=0.664), body mass index (p=0.601), and pediatric pre-existing chronic conditions (p=1.000). The Pediatric Quality of Live Inventory 4.0 scores, median physical score (69 [0-100] versus 81 [34-100], p=0.012), and school score (60 [15-100] versus 70 [15-95], p=0.028) were significantly lower in pediatric patients with COVID-19 at the longitudinal follow-up visit than in controls. CONCLUSIONS: Pediatric patients with COVID-19 showed a longitudinal impact on HRQoL parameters, particularly in physical/school domains, reinforcing the need for a prospective multidisciplinary approach for these patients. These data highlight the importance of closer monitoring of children and adolescents by the clinical team after COVID-19.

https://doi.org/10.6061/clinics/2021/e3511

Poor Sleep quality and health-related quality of life impact in adolescents with and without chronic immunosuppressive conditions during COVID-19 quarantine
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Helito, Alberto C.

; Lindoso, Livia

; Sieczkowska, Sofia M.

; Astley, Camilla

; Queiroz, Ligia B.

; Rose, Natalia

; Santos, Claudia Renata P.

; Bolzan, Thalis

; Peralta, Rita María I.A.

; Franco, Ruth R.

; Cominato, Louise

; Pereira, Rosa Maria R.

; Tannuri, Uenis

; Campos, Lucia Maria A.

; Lourenço, Benito

; Toma, Ricardo K.

; Medeiros, Karina

; Watanabe, Andréia

; Grangeiro, Patricia Moreno

; Farhat, Sylvia C.

; Casella, Caio B.

; Polanczyk, Guilherme V.

; Gualano, Bruno

; Silva, Clovis A.

; Sallum, Adriana M. E. Iraha, Amanda Y. Ihara, Bianca P. Mazzolani, Bruna C. Martinez, Claudia A. Strabelli, Claudia A. A. Fonseca, Claudia B. Lima, Dandara C. C. Setoue, Debora N. D. Roz, Deborah F. P. Smaira, Fabiana I. Roschel, Hamilton Miyatani, Helena T. Marques, Isabela G. Oba, Jane Ferreira, Juliana C. O. Simon, Juliana R. Kozu, Katia Saccani, Ligia P. Martiniano, Lorena V. M. Miranda, Luana C. A. Silva, Luiz E. V. Laurentino, Moisés F. Aikawa, Nadia E. Sakita, Neusa K. Tanigava, Nicolas Y. Pereira, Paulo R. A. Palmeira, Patrícia Angelo, Simone S. Lavorato, Sofia S. M. Bernardes, Tamires M. Franco, Tathiane C. Viana, Vivianne S. L. Barros, Vera P. M. F. R. Zheng, Yingying

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2021, Volume 76 elocation e3501

Resumo: En | Texto: En | PDF: En

OBJECTIVE: To assess the possible factors that influence sleep quality in adolescents with and without chronic immunosuppressive conditions quarantined during the coronavirus disease 2019 (COVID-19) pandemic. METHODS: This cross-sectional study included 305 adolescents with chronic immunocompromised conditions and 82 healthy adolescents. Online surveys were completed, which included questions on socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and the following validated questionnaires: the Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0), and Pediatric Outcome Data Collection Instrument (PODCI). RESULTS: The median current age [14 (10-18) vs. 15 (10-18) years, p=0.847] and frequency of female sex (62% vs. 58%, p=0.571) were similar in adolescents with chronic conditions compared with healthy adolescents. The frequency of poor sleep quality was similar in both groups (38% vs. 48%, p=0.118). Logistic regression analysis, including both healthy adolescents and adolescents with chronic conditions (n=387), demonstrated that self-reported increase in screen time (odds ratio [OR] 3.0; 95% confidence interval [CI] 1.3-6.8; p=0.008) and intrafamilial violence report (OR 2.1; 95% CI 1.2-3.5; p=0.008) were independently associated with poor sleep quality in these adolescents. However, the PODCI global function score was associated with a lower OR for poor sleep quality (OR 0.97; 95% CI 0.94-0.99; p=0.001). Further logistic regression, including only adolescents with chronic conditions (n=305), demonstrated that self-reported increase in screen time (OR 3.1; 95% CI 1.4-6.8; p=0.006) and intrafamilial violence report (OR 2.0; 95% CI 1.2-3.4; p=0.011) remained independently associated with poor quality of sleep, whereas a lower PODCI global function score was associated with a lower OR for sleep quality (OR 0.96; 95% CI 0.94-0.98; p<0.001). CONCLUSION: Self-reported increases in screen time and intrafamilial violence report impacted sleep quality in both healthy adolescents and those with chronic conditions. Decreased health-related quality of life was observed in adolescents with poor sleep quality.

https://doi.org/10.6061/clinics/2021/e3501

Lower genital tract infections in young female juvenile idiopathic arthritis patients
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Ferreira, Gabriela R. V.

; Tomioka, Renato B. Queiroz, Ligia B. Kozu, Katia Aikawa, Nadia E. Sallum, Adriana M. E. Serafini, Paulo Tacla, Maricy Baracat, Edmund C. Pereira, Rosa M. R. Bonfá, Eloisa Silva, Clovis A.

Advances in Rheumatology

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2019, Volume 59 elocation 50

Resumo: En | Texto: En | PDF: En

Abstract Background: To evaluate human papillomavirus (HPV), Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) infections in juvenile idiopathic arthritis (JIA) patients. Methods: After exclusion, 33 female adolescent and young JIA patients (ILAR criteria) and 28 healthy controls were selected for this study. Demographic data, gynecological, sexual function, cervical cytology and histological abnormalities were evaluated. JIA clinical/laboratorial parameters and treatment were also assessed. HPV-DNA, CT-DNA and NG-DNA testing in cervical specimens were performed by Hybrid Capture 2 assays. Results: The mean current age was similar in JIA patients and controls (23.3 ± 6.24 vs. 26.1 ± 6.03 years, p = 0.09). The frequencies of sexual intercourse (76% vs. 89%, p = 0.201) and abnormal cervical cytology (24% vs. 11%, p = 0.201) were similar in JIA compared to controls. The higher frequency of HPV infection in JIA patients than controls (30% vs. 11%, p = 0.155) did not reach statistical significance. CT (0% vs. 7%, p = 0.207) and NG infections (0% vs. 4%, p = 0.459) were also alike in both groups. Further evaluation of JIA patients with abnormal and normal cervical cytology showed that the former group had a higher frequency of HPV infection (87% vs. 12%, p = 0.0002) with a low frequency of HPV vaccination (0% vs. 8%, p = 1.0). No differences were evidenced between these two JIA groups regarding demographic data, sexual function and clinical/laboratorial parameters. The frequencies of methotrexate (p =0.206) and biological agent use (p =0.238) were similar in both JIA groups. Conclusions: To our knowledge, this was the first study to assess lower genital infections in JIA patients allowing the identification of HPV as main cause of cervical dysplasia. Methotrexate and biological agents do not seem to increase risk of lower genital tract infections in JIA patients.

https://doi.org/10.1186/s42358-019-0092-6 407 downloads

10.

Methicillin-resistant Staphylococcus aureus isolated from an intensive care unit in Minas Gerais, Brazil, over a six-year period
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Nascimento, Thiago C. Diniz, Cláudio G. Silva, Vânia L. Ferreira-Machado, Alessandra B. Fajardo, Marina O. de Oliveira, Tamara Lopes R. Ferreira, Dennis de C. Cavalcante, Fernanda S. Santos, Kátia R. Netto dos

Brazilian Journal of Infectious Diseases

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Jan 2018, Volume 22 Nº 1 Páginas 55 - 59

Resumo: En | Texto: En | PDF: En

ABSTRACT To characterize methicillin-resistant Staphylococcus aureus isolates from an intensive care unit of a tertiary-care teaching hospital, between 2005 and 2010. A total of 45 isolates were recovered from patients admitted to the intensive care unit in the study period. Resistance rates higher than 80% were found for clindamycin (100%), erythromycin (100%), levofloxacin (100%), azithromycin (97.7%), rifampin (88.8%), and gentamycin (86.6%). The SCCmec typing revealed that the isolates harbored the types III (66.7%), II (17.8%), IV (4.4%), and I (2.2%). Four (8.9%) isolates carried non-typeable cassettes. Most (66.7%) of the isolates were related to the Brazilian endemic clone from CC8/SCCmec III, which was prevalent (89.3%) between 2005 and 2007, while the USA100/CC5/SCCmec II lineage emerged in 2007 and was more frequent in the last few years. The study showed high rates of antimicrobial resistance among methicillin-resistant S. aureus isolates and the replacement of Brazilian clone, a well-established hospital lineage, by the USA100 in the late 2000s, at the intensive care unit under study.

https://doi.org/10.1016/j.bjid.2017.10.004 501 downloads

11.

Autoimmune hepatitis in 847 childhoodonset systemic lupus erythematosus population: a multicentric cohort study
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Balbi, Verena A.

; Montenegro, Bárbara Pitta, Ana C. Schmidt, Ana R. Farhat, Sylvia C. Coelho, Laila P. Ferreira, Juliana C. O. Pereira, Rosa M. R. Terreri, Maria T. Saad-Magalhães, Claudia Aikawa, Nadia E. Sakamoto, Ana P. Kozu, Kátia Campos, Lucia M. Sallum, Adriana M. Ferriani, Virginia P. Piotto, Daniela P. Bonfá, Eloisa Silva, Clovis A.

Advances in Rheumatology

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2018, Volume 58 elocation 43

Resumo: En | Texto: En | PDF: En

Abstract Objective: To evaluate autoimmune hepatitis (AIH) in a multicenter cohort of childhood-onset systemic lupus erythematosus (cSLE) patients. Methods: This retrospective multicenter study included 847 patients with cSLE, performed in 10 Pediatric Rheumatology services of São Paulo state, Brazil. AIH was defined according to the International Autoimmune Hepatitis Group criteria (IAHGC). The statistical analysis was performed using the Bonferroni's correction (p < 0.0033). Results: AIH in cSLE patients confirmed by biopsy was observed in 7/847 (0.8%) and all were diagnosed during adolescence. The majority occurred before or at cSLE diagnosis [5/7 (71%)]. Antinuclear antibodies were a universal finding, 43% had concomitantly anti-smooth muscle antibodies and all were seronegative for anti-liver kidney microsomal antibodies. All patients with follow-up ≥18 months (4/7) had complete response to therapy according to IAHGC. None had severe hepatic manifestations such as hepatic failure, portal hypertension and cirrhosis at presentation or follow-up. Further comparison of 7 cSLE patients with AIH and 28 without this complication with same disease duration [0 (0–8.5) vs. 0.12 (0–8.5) years, p = 0.06] revealed that the frequency of hepatomegaly was significantly higher in cSLE patients in the former group (71% vs. 11%, p = 0.003) with a similar median SLEDAI-2 K score [6 (0–26) vs. 7 (0–41), p = 0.755]. No differences were evidenced regarding constitutional involvement, splenomegaly, serositis, musculoskeletal, neuropsychiatric and renal involvements, and treatments in cSLE patients with and without AIH (p > 0.0033). Conclusions: Overlap of AIH and cSLE was rarely observed in this large multicenter study and hepatomegaly was the distinctive clinical feature of these patients. AIH occurred during adolescence, mainly at the first years of lupus and it was associated with mild liver manifestations.

https://doi.org/10.1186/s42358-018-0043-7 473 downloads

12.

Juvenile dermatomyositis: is periodontal disease associated with dyslipidemia?
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Kozu, Kátia T. Silva, Clovis A. Aikawa, Nadia E. Pereira, Rosa M. R. Sallum, Adriana M. Savioli, Cynthia Borba, Eduardo Campos, Lucia M.

Advances in Rheumatology

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2018, Volume 58 elocation 28

Resumo: En | Texto: En | PDF: En

Abstract Background: Association between periodontal disease and dyslipidemia was recently reported in healthy adults. However, a systematic evaluation of concomitant periodontal diseases and lipid profile was not carried out in juvenile dermatomyositis (JDM). A cross-section study was performed in 25 JDM patients and 25 healthy controls, assessing demographic data, periodontal evaluation, fasting lipoproteins and anti-lipoprotein lipase antibodies. Disease parameters, laboratorial tests and treatment were also evaluated in JDM patients. Results: The mean current age was similar in patients and controls (11.5 ± 3.75 vs. 11.2 ± 2.58 years, p = 0.703). Regarding lipid profile, the median triglycerides [80(31–340) vs. 61(19–182)mg/dL, p = 0.011] and VLDL[16(6–68) vs. 13(4–36)mg/dL,p = 0.020] were significantly higher in JDM patients versus controls. Gingival vasculopathy pattern was significantly higher in the former group (60% vs. 0%, p = 0.0001), as well as the median of gingival bleeding index (GBI) [24.1(4.2–69.4) vs. 11.1(0–66.6)%,p = 0.001] and probing pocket depth (PPD) [1.7(0.6–2.4) vs.1.4(0–2.12) mm,p = 0.006]. Comparison between JDM patients with and without dyslipidemia revealed that the median of dental plaque index (PI) [100(26.7–100) vs. 59(25–100)%,p = 0.022], PPD[1.9(0.6–2.4) vs. 1.4(1.2–1.8)mm,p = 0.024] and clinical attachment level (CAL) [1.31(0.7–1.7) vs. 0.8(0.6–1.7)mm,p = 0.005] were significantly higher in patients with dyslipidemia. Further analysis between JDM patients with and without gingivitis revealed that the median of current age [12.4 (8.3–18.4) vs. 9.2 (5.5–17.5) years, p = 0.034] and disease duration [7.09 ± 3.07 vs. 3.95 ± 2.1 years, p = 0.008] were significantly higher in the former group. Conclusion: Our study showed that gingival inflammation seems to be related to dyslipidemia in JDM patients, suggesting underlying mechanisms for both complications.

https://doi.org/10.1186/s42358-018-0024-x 289 downloads

13.

Human-modified landscape acts as refuge for mammals in Atlantic Forest
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Bovo, Alex Augusto de Abreu

; Magioli, Marcelo

; Percequillo, Alexandre Reis

; Kruszynski, Cecilia

; Alberici, Vinicius

; Mello, Marco A. R.

; Correa, Lidiani Silva

; Gebin, João Carlos Zecchini

; Ribeiro, Yuri Geraldo Gomes

; Costa, Francisco Borges

; Ramos, Vanessa Nascimento Benatti, Hector Ribeiro Lopes, Beatriz Martins, Maísa Z. A. Diniz-Reis, Thais Rovere Camargo, Plínio Barbosa de Labruna, Marcelo Bahia Ferraz, Katia Maria Paschoaletto Micchi de Barros

Biota Neotropica

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2018, Volume 18 Nº 2 elocation e20170395

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo: Paisagens antropicamente modificadas (HMLs) são compostas por fragmentos florestais pequenos, isolados e defaunados, imersos em áreas agrícolas e/ou urbanas. Informações sobre as espécies que habitam essas paisagens são importantes para o direcionamento de estratégias de conservação em escalas local e regional. Uma vez que as HMLs são as paisagens dominantes na Mata Atlântica, o objetivo deste trabalho foi avaliar a diversidade de mamíferos em uma HML do sudeste do Brasil e propor estratégias para sua conservação. Foram coletados dados de mamíferos terrestres (pequenos, médios e grandes) e voadores em três fragmentos florestais (10, 14 e 26 ha) e áreas adjacentes, entre 2003 e 2016, usando métodos complementares: busca ativa, armadilhamento fotográfico, armadilhas de captura e redes de neblina. Adicionalmente, foram utilizados dados de literatura para complementar a lista de espécies. Foram registradas 35 espécies de mamíferos nativos (6 de pequenos, 16 de médios e grandes e 13 de morcegos) e sete espécies exóticas. A assembleia de mamíferos registrada (terrestres e voadores), embora composta por espécies generalistas, apresentou três espécies de médio e grande porte ameaçadas de extinção nacionalmente (Leopardus guttulus, Puma concolor and Puma yagouaroundi) e duas deficientes em dados (Galictis cuja and Histiotus velatus), destacando a importância dessa HML para conservação e manutenção das populações de mamíferos. Embora inserida em uma paisagem extremamente modificada, a área de estudo abriga uma riqueza significativa de mamíferos de médio e grande porte, sendo um importante refúgio para a biodiversidade na região. Entretanto, essa biodiversidade está ameaçada pela baixa qualidade dos habitats, por atropelamentos e por abundantes populações de cães e gatos domésticos. Portanto, enfatizamos a necessidade de estratégias de conservação focadas nos mamíferos de médio e grande porte como grupo "guarda-chuva", o que pode beneficiar as demais espécies na paisagem. Recomendamos ações de conservação visando a restauração biológica, para melhorar a composição estrutural e conectividade dos fragmentos florestais, reduzir o número de atropelamentos e controlar as populações de cães e gatos domésticos, afim de manter e aumentar a diversidade local de mamíferos em longo prazo.

Abstract: Human-modified landscapes (HMLs) are composed by small, isolated and defaunated forest fragments, which are surrounded by agricultural and urban areas. Information on species that thrives in these HMLs is essential to direct conservation strategies in local and regional scales. Since HMLs are dominant in the Atlantic Forest, we aimed to assess the mammalian diversity in a HML in southeastern Brazil and to propose conservation strategies. We collected data of terrestrial (small-, medium- and large-sized) and volant mammals in three small forest fragments (10, 14 and 26 ha) and adjacent areas, between 2003 and 2016, using complementary methods: active search, camera trapping, live-traps, mist nets and occasional records (i.e., roadkills). In addition, we used secondary data to complement our species list. We recorded 35 native mammal species (6 small-sized, 16 medium- and large-sized, and 13 bats) and seven exotic species in the HML. The recorded mammal assemblage (non-volant and volant), although mainly composed of common and generalist species, includes three medium- and large-sized species nationally threatened (Leopardus guttulus, Puma concolor and Puma yagouaroundi) and two data deficient species (Galictis cuja and Histiotus velatus), highlighting the importance of this HML for the maintenance and conservation of mammal populations. Despite highly impacted by anthropogenic disturbances, the study area harbors a significant richness of medium- and large-sized mammals, being an important biodiversity refuge in the region. However, this biodiversity is threatened by the low quality of the habitats, roadkills and abundant populations of domestic cats and dogs. Therefore, we stress the need of conservation strategies focusing on the medium- and large-sized mammals as an umbrella group, which could benefit all biodiversity in the landscape. We recommend actions that promotes biological restoration, aiming to increase structural composition and connectivity of the forest fragments, reducing roadkills and controlling the domestic cats and dogs' populations, in order to maintain and improve the diversity of mammals in long-term.

https://doi.org/10.1590/1676-0611-bn-2017-0395 3596 downloads

14.

Effect of Endophytic Fungal Associations on the Chemical Profile of in vitroVochysia divergens Seedlings
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Parpinelli, Bruna A. S. Siqueira, Katia A. Kellner Filho, Luis C. Pimenta, Letícia P. Costa, Ricardo M. da Parreira, Renato L. T. Veneziani, Rodrigo C. S. Silva, Marcio L. Andrade e Cunha, Wilson R. Pauletti, Patrícia M. Soares, Marcos A. Januario, Ana H.

Journal of the Brazilian Chemical Society

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Dez 2017, Volume 28 Nº 12 Páginas 2375 - 2381

Resumo: En | Texto: En | PDF: En

Vochysia divergens (Vochysiaceae) is considered an invasive species in the wetlands of the Brazilian Pantanal, which hinders the cultivation of agricultural species. In this study, we evaluated the chemical profile by HPLC-DAD (high-performance liquid chromatography-diode array detector) of leaves extracts from V. divergens seedlings inoculated with endophytic fungi isolated from V. divergens roots. These fungi were collected on dry (D) and wet (W) seasons in the Pantanal. The presence of tannin hexahydroxydiphenoyl (HHDP)-galloyl-glucose and flavone 3',5'-dimethoxy-luteolin were predominant in the seedlings inoculated with endophytic fungi W experiments at 100 and 80%, respectively. Likewise, flavone 3',5-dimethoxy-luteolin-7-O-β-glucoside showed a similar representation in the two evaluated periods, compared with 5-methoxy-luteolin, which was detected only in seedlings inoculated with W endophytic fungi. This approach is new to V. divergens, which has no scientific data on its in vitro elicitation, in the search for a better understanding of the ecological relationships of this species.

https://doi.org/10.21577/0103-5053.20170091 597 downloads

15.

Disseminated histoplamosis in adolescent mimicking granulomatosis with polyangiitis
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van Weelden, Marlon Viola, Gabriela R. Kozu, Katia T. Aikawa, Nadia E. Ivo, Claudia M. Silva, Clovis A.

Revista Brasileira de Reumatologia

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Out 2017, Volume 57 Nº 5 Páginas 479 - 482

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1016/j.rbre.2015.01.003 849 downloads

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