ABSTRACT The objective of this study was to evaluate the effects of replacing choline chloride with a plant source of choline on the locomotor system, liver health, and development of breast myopathies in broilers aged 1-42 days of age. We allocated 1,120 one-day-old Cobb broilers to four treatment groups and fed them commercial diets based on corn and soybean meal. The treatments included choline in the form of 1,800.00 mg/kg choline chloride; 1,350.00 mg/kg choline chloride + 450.00 mg/kg herbal choline; 900.00 mg/kg choline chloride + 900.00 mg/kg herbal choline; and 1,000.00 mg/kg herbal choline. Each treatment group had eight replications. Throughout the experiment, gait score, footpad dermatitis, hock burn, and leg deformities (valgus and varus) were evaluated in the birds at 28 and 35 days of age. After slaughter, parameters such as breast myopathies, tibial dyschondroplasia score, and histological slides of the pectoral muscle, liver, and proximal tibial epiphysis were assessed. The results demonstrated good hepatic and locomotor health in the broilers, as no classical signs of choline deficiency were observed. Statistical analyses indicated no significant differences between treatments in terms of liver and locomotor health, suggesting that broilers fed diets supplemented with the plant source did not experience choline deficiency. Additionally, no statistically significant differences were found between treatments regarding breast myopathies. Overall, the tested choline plant source can effectively replace choline chloride in broiler diets. system 142 1 42 1-4 age 1120 120 1,12 onedayold one day old meal 180000 800 00 1,800.0 mgkg mg kg 135000 350 1,350.0 45000 450 450.0 90000 900 900.0 100000 000 1,000.0 replications experiment score dermatitis burn valgus varus 2 3 slaughter muscle assessed observed Additionally Overall 14 4 1- 112 12 1,1 18000 80 0 1,800. 13500 1,350. 4500 45 450. 9000 90 900. 10000 1,000. 11 1, 1800 8 1,800 1350 1,350 9 1000 1,000 180 1,80 135 1,35 100 1,00 18 1,8 13 1,3 10 1,0

RESUMO: Varronia curassavica é uma espécie medicinal nativa no Brasil e sua propriedade anti-inflamatória é atribuída aos compostos α-humuleno e β-cariofileno. O objetivo foi estudar a variabilidade de população de V. curassavica. Foram coletadas folhas de 40 indivíduos de população natural, sendo estas transferidas para frascos de vidro (2 mL). Os voláteis foram extraídos e analisados por headspace estático, associado à cromatografia gasosa, acoplada à espectrometria de massas (HS-CG-EM). Detectou-se 17 compostos: monoterpenos (1,6% - 68%) e sesquiterpenos (25,7% - 79,0%), incluindo o α-humuleno e o β-cariofileno. A análise multivariada foi realizada para estimar a dissimilaridade dos indivíduos, a qual separou quatro grupos. O grupo I foi caracterizado pela presença do α-pineno, os grupos II e IV do β-cariofileno e o grupo III do β-pineno. O teor do α-humuleno apresentou pequenas variações, enquanto o do β-cariofileno contribuiu mais para a variabilidade da população. É o primeiro relato de análise da composição química de compostos voláteis de V. curassavica utilizando a técnica HS-GC-MS.

ABSTRACT: Varronia curassavica is a medicinal species native to Brazil; its anti-inflammatory properties are attributed to α-humulene and β-caryophyllene. This study to investigated the population variability of V. curassavica. Leaves were collected from 40 individuals of a natural population and transferred to glass flasks (2 mL). The volatiles were extracted and analyzed using static headspace gas chromatography coupled with mass spectrometry (HS-GC-MS). A total of 17 compounds were detected: monoterpenes (1.6% - 68%) and sesquiterpenes (25.7% - 79.0%), including α-humulene and β-caryophyllene. Multivariate analysis was performed to estimate the dissimilarity of individuals separated the compounds into four groups. Group I was characterized by the content of α-pinene, groups II and IV by β-caryophyllene, and group III by β-pinene. The abundance of α-humulene showed small variations; while β-caryophyllene showed larger variations that contributed to the population variability. This is the first report on the chemical composition analysis of volatile compounds in V. curassavica using the HS-GC-MS technique.

Resumo O objetivo deste trabalho foi avaliar o crescimento e o teor de óleo essencial após transplantio e corte de acessos de Lippia rotundifolia provenientes de população natural do estado de Minas Gerais, Brasil. Avaliaram-se oito acessos (PVP, GIG, RPE, ODA, JFE, PRP, SGS e RTI), em seis períodos (60, 81, 102, 123, 144 e 165 dias após transplantio e corte), em delineamento experimental inteiramente casualizado. Foram avaliados os seguintes parâmetros: largura média e comprimento do par de folhas basal, mediano e apical; altura da planta; diâmetro do caule; e número de folhas. Aos 165 dias, também avaliaram-se produção de óleo essencial e fitomassa fresca e seca da parte aérea. Sessenta dias após a última avaliação, mensurou-se a rebrota a partir de altura, diâmetro do caule e número de folhas. O acesso JFE se destaca na emissão de folhas, com melhor desenvolvimento aos 165 dias após o transplantio. RPE e PRP respondem bem à rebrota aos 102 e 123 dias após o corte, respectivamente. O acesso SGS apresenta alta produção de óleo essencial de 2,2%, e o PVP, baixa produção de 0,3%.

Abstract The objective of this work was to evaluate the growth and essential oil content after transplantation and cutting of Lippia rotundifolia accessions from a natural population from the state of Minas Gerais, Brazil. Eight accessions were evaluated (PVP, GIG, RPE, ODA, JFE, PRP, SGS, and RTI) in six periods (60, 81, 102, 123, 144, and 165 days after transplanting and cutting), in a completely randomized experimental design. The following parameters were assessed: average width and length of the basal, median, and apical pair of leaves; plant height; stem diameter; and number of leaves. At 165 days, the yield of essential oil and the fresh and dry phytomass of the aerial part were also evaluated. Sixty days after the last evaluation, regrowth was measured from height, stem diameter, and number of leaves. The JFE accession stands out for emission of leaves, with a better development at 165 days after transplanting. RPE and PRP respond well to regrowth at 102 and 123 days after cutting, respectively. The SGS accession shows a high essential oil yield of 2.2%, and PVP, a low yield of 0.3%.

Resumo Estima-se que cerca de 28 milhões de cirurgias sejam postergadas ou canceladas nomundo em decorrência desta pandemia, causando atraso no diagnóstico e tratamento de mais de 2 milhões de casos oncológicos. No Brasil, tanto a ANS (Agencia Nacional de Saúde) comoa ANVISA (Agencia Nacional de Vigilância Sanitária) orientaram o adiamento das cirurgias eletivas e não essenciais, tendo um impacto considerável no número de procedimentos cirúrgicos comdiminuição de 33,4% neste período no Brasil.No entanto, algumasmulheres necessitam de tratamento para várias doenças ginecológicas, algumas das quais não podem ser adiadas. O objetivo deste artigo é apresentar recomendações sobre o tratamento cirúrgico durante a pandemia de COVID-19.

Abstract It is estimated that around 28 million surgeries will be postponed or canceled worldwide as a result of this pandemic, causing a delay in the diagnosis and treatment of more than 2 million cancer cases. In Brazil, both the National Health Agency (ANS) and National Health Surveillance Agency (ANVISA) advised the postponement of elective and non-essential surgeries, causing a considerable impact on the number of surgical procedures that decreased by 33.4% in this period. However, some women need treatment for various gynecological diseases that cannot be postponed. The purpose of this article is to present recommendations on surgical treatment during the COVID-19 pandemic.

Abstract Au/TiO2 (1wt% Au), Cu/TiO2 (1wt% Cu) and AuCu/TiO2 (1wt% AuCu) catalysts with different Au:Cu mass ratios were prepared in one-step synthesis using sodium borohydride as reducing agent. The resulting catalysts were characterized by X-ray diffraction (XRD), X-ray Dispersive Energy (EDX), Transmission Electron Microscopy (TEM) and Temperature Programmed Reduction (TPR) and tested for the preferential oxidation of carbon monoxide (CO-PROX reaction) in H2-rich gases. EDS analysis showed that the Au contents are close to the nominal values whereas for Cu these values are always lower. X-ray diffractograms showed only the peaks of TiO2 phase; no peaks of metallic Au and Cu species or oxides phases were observed. TPR and high-resolution TEM analysis showed that AuCu/TiO2 catalysts exhibited most of Au in the metallic form with particles sizes in the range of 3-5 nm and that Cu was found in the form of oxide in close contact with the Au nanoparticles and well spread over the TiO2 surface. The AuCu/TiO2 catalysts exhibited good performance in the range of 75-100 °C and presented a better catalytic activity when compared to the monometallic ones. A maximum CO conversion of 98.4% with a CO2 selectivity of 47% was obtained for Au0.50Cu0.50/TiO2 catalyst at 100oC.

Abstract Macrocryst assemblages of porphyritic alkaline dikes in the Mantiqueira range (SE Brazil) are mainly composed of clinopyroxene and olivine with different origins. Based on petrographic features, mineral chemistry, and equilibrium relationships with the host liquid, those macrocrysts are classified as xenocrysts, antecrysts, and phenocrysts. Described xenocrysts are mantle olivine, Cr-diopside cores compatible with garnet-bearing mantle facies, green-core clinopyroxene cores compatible with lower crust, and enstatite cores mantled by clinopyroxene, all reported for the first time in this region. Two contrasting types of clinopyroxene antecrysts prevail among the macrocryst cores (both occurring in the same samples and presenting corrosion and sieve textures): primitive colorless diopside and more evolved green-core clinopyroxenes. In the studied rocks, green clinopyroxene zones mantling colorless diopside cores (and vice-versa) are also found. Diopside- and green-cores antecrysts have similar compositions to those from mafic and felsic alkaline melts, respectively. Phenocrysts are mainly related to Ti-augite overgrowths, mantling all other types. Mixing-model curves between mafic and felsic alkaline equilibrium liquids calculated from clinopyroxene antecrysts indicate a hybrid origin for the host matrix. The macrocryst populations of the studied dikes are indicative of a complex plumbing system, recording several processes of an open-system magmatic evolution.

RESUMO: A atividade conservante e antioxidante de óleos essenciais são importantes ferramentas para uso na indústria de alimentos e pesquisas são estimuladas na atualidade. O objetivo deste trabalho foi avaliar as propriedades antioxidantes e a atividade antimicrobiana de óleos essenciais de Syzygium aromaticum, Cymbopogon citratuse Lippia alba (LA) contra bactérias lácticas e patogênicas com importância em doenças transmitidas por alimentos. A atividade antibacteriana dos óleos foi avaliada por testes de disco-difusão e macrodiluição em caldo, utilizando-se cultura láctica mista de Lactobacillus delbrueckii subsp. bulgaricus e Streptococcus thermophilus (YF-L903), e Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538), Salmonella enterica (ATCC 6017). Os compostos dos óleos essenciais foram identificados por cromatografia, mostrando eugenol (79,41%) como composto predominante em S. aromaticum, geranial (31,89%), neral (24,52%) e β-mirceno (25,37%) em C. citratus, e o geranial (33,80%) e neral (25,63%) em L. alba. A atividade antibacteriana confirmou o efeito dose-dependente dos três óleos para todas as bactérias avaliadas, apresentando halos de inibição a partir da concentração de 20μL mL-1. O óleo essencial de S. aromaticum apresentou melhor atividade antioxidante, com IC 50 IC50 (at concentration capable of generating 50% inhibition) igual a 5.76μg mL-1 e índice de atividade antioxidante (AAI) de 6,94, considerado forte (AAI>2,0) em relação aos demais óleos avaliados. O óleo essencial de S. aromaticum apresentou excelente atividade antioxidante em menor concentração que anecessário para inibir a cultura láctica, indicando que este óleo pode ser usado como conservante em alimentos processados contendo culturas lácticas.

ABSTRACT: Studies about preservative and antioxidant activity of essential oils have been encouraged in recent years, given their importance to food industry. The aim of the current study was to evaluate the antioxidant properties and antimicrobial activity of essential oils deriving from Syzygium aromaticum, Cymbopogon citratus and Lippia alba against lactic and pathogenic bacteria responsible for food-borne diseases. Essential oil antibacterial activity was assessed through disc diffusion and macrodilution tests conducted in a mixed lactic culture of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus (YF-L903) and of Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538), Salmonella enterica (ATCC 6017) strains. Based on the chromatographic analysis results, the essential oils shown to be composed of eugenol (79.41%) which was the prevalent compound in S. aromaticum, geranial (31.89%), neral (24.52%) and β-myrcene (25.37%) in C. citratus, as well as of geranial (33.80%) and neral (25.63%) in L. alba. The observed antibacterial activity confirmed the dose-dependent effect of these three oils on all the assessed bacteria; there was halo inhibition at concentration 20μL mL-1. The essential oil of S. aromaticum presented better antioxidant activity, with IC50 equal to 5.76μg mL-1 and antioxidant activity index of 6.94, and it was considered strong (AAI>2.0) in comparison to the other evaluated oils. This essential oil also presented excellent antioxidant activity at concentrations lower than the one required to inhibit lactic cultures. Based in this outcome, the essential oil from S. aromaticum can be used as preservative agent in processed food whose formulation presents lactic cultures.

RESUMO O objetivo deste estudo foi comparar a eficiência de remoção de DQO de um Venturi e de uma placa de orifício. Adicionalmente, a otimização da pressão de entrada dos dispositivos, o estudo da cinética da degradação, e o efeito sinérgico resultante da combinação da cavitação e H2O2 foram realizados. Um aparato experimental em escala laboratorial foi construído para atingir esses objetivos. Uma solução de sacarose e um efluente real de uma indústria de refrigerante, a base de sacarose, foram tratados. Os resultados mostraram que o Venturi atingiu 90% de remoção de DQO em apenas em três minutos de tratamento, com a pressão de 4,0 bar e proporção molar de sacarose: peróxido de 1:20. Enquanto que, para placa de orifício, uma eficiência de 90% foi atingida em 9 min, na pressão de 2,0 bar. A ordem de reação da cinética de degradação foi igual a 3,5, com exceção da maior pressão de entrada do Venturi (7,3 bar) na qual ocorreu coalescência das cavidades e a redução na velocidade de degradação. O coeficiente sinérgico (Sc) foi igual a 185,20, mostrando que o processo híbrido (HC + H2O2) é muito mais eficiente do que a cavitação isolada. A elevada concentração de DQO do efluente da indústria de refrigerante (2.512,8 mg L-1) foi degradada no Venturi com eficiência de 72%, sob uma pressão de entrada de 4,0 bar e baixa proporção (sacarose: peróxido de 1:10). O sistema de cavitação com dispositivo Venturi tem grande potencial para remover elevadas concentrações de matéria orgânica em um curto período de tratamento.

ABSTRACT The aim of the current study is to compare the chemical oxygen demand (COD) removal efficiency of a Venturi device to that of an orifice plate. The inlet pressure in the devices was optimized and the degradation kinetics was analyzed. In addition, the synergistic effect resulting from the combination between cavitation and H2O2 was investigated. An experimental apparatus was built to achieve these goals. A sucrose solution and an effluent from a sucrose-based soft drink industry were treated. Results showed that the Venturi device recorded 90% COD removal efficiency after three treatment minutes. On the other hand, the orifice plate recorded 90% COD removal efficiency after 9 min. The degradation kinetics-reaction order was 3.5, except for the highest Venturi inlet pressure (7.3 bar), which led to coalescence of cavities and to reduced degradation rate. The synergistic coefficient (Sc) was 185.20 and showed that the hybrid process (HC + H2O2) was much more efficient than the cavitation process applied alone. The high COD concentration in the effluent generated by the soft drink industry (2,512.8 mg L-1) was significantly decreased by 72%. The combined use of cavitation and Venturi has significant potential to remove high organic matter concentrations in short treatment periods.

Resumo: O objetivo deste trabalho foi avaliar os efeitos da aplicação parcelada e combinada de zinco foliar (ZnF) + Zn no solo (ZnS) sobre o teor de Zn dos grãos (TZnG) do feijão-comum (Phaseolus vulgaris). Dois experimentos foram realizados em casa de vegetação durante dois períodos. No primeiro experimento, duas cultivares de feijão-comum receberam seis doses de ZnF (0, 120, 240, 480, 720, ou 1.200 g ha-1), com aplicações parceladas nos estágios da planta R5, R7 e R8, ou R7 e R8, ou R8. No segundo experimento, uma cultivar recebeu três doses de ZnS (0, 5 ou 10 kg ha-1) combinadas com doses de ZnF (0, 1, 1,5, 3, 6 ou 10 kg ha-1). No primeiro experimento, com o incremento do ZnF, o TZnG aumentou linearmente de 15,3 para 20,7 mg kg-1. O parcelamento do ZnF não afetou o TZnG. No segundo experimento, o ZnF sem o ZnS não afetou o TZnG. O ZnS dobrou o TZnG em comparação ao tratamento sem aplicação de ZnS. Com 5 kg ha-1 de ZnS, o mais alto TZnG (67,5 mg kg-1) foi obtido com 7,8 kg ha-1 de ZnF. Com 10 kg ha-1, o mais alto TZnG (66,0 mg kg-1) foi obtido com 4,9 kg ha-1 de ZnF. O parcelamento do ZnF não é vantajoso em relação à aplicação única, e a combinação ZnF e ZnS aumenta o TZnG do feijão-comum em casa de vegetação.

Abstract: The objective of this work was to evaluate the effects of the split and combinated application of foliar zinc (ZnF) + soil Zn (ZnS) on Zn concentration in the grains (ZnCG) of common bean (Phaseolus vulgaris). Two experiments were carried out in a greenhouse during two seasons. In the first experiment, two common bean cultivars received six ZnF rates (0, 120, 240, 480, 720, or 1,200 g ha-1), with split applications at the R5, R7, and R8, or R7 and R8, or R8 plant stages. In the second experiment, one cultivar received three ZnS rates (0, 5, or 10 kg ha-1) combined with ZnF rates (0, 1, 1.5, 3, 6, or 10 kg ha-1). In the first experiment, with the ZnF increment, ZnCG increased linearly from 15.3 to 20.7 mg kg-1. ZnF splitting did not affect ZnCG. In the second experiment, ZnF without ZnS did not affect ZnCG. ZnS doubled ZnCG in comparison with the treatment without ZnS application. At 5 kg ha-1 ZnS, the highest ZnCG (67.5 mg kg-1) was obtained with 7.8 kg ha-1 ZnF. At 10 kg ha-1, the highest ZnCG (66 mg kg-1) was obtained with 4.9 kg ha-1 ZnF. ZnF splitting is not advantageous over a single application, and the combination of ZnF and ZnS increases ZnCG in common bean, in greenhouse conditions.

ABSTRACT: Three series of alkaline dikes are characterized in a restricted area of the Mantiqueira Range: a weakly silica-undersaturated series (normative ne < 7), of potassic character, represented by basanite, tephrites, alkali basalts, basaltic trachyandesites, phonotephrites, tephriphonolites, and trachyandesites, an intermediate series (7 > normative ne > 13), of potassic character, consisting only of basanites and tephrites, and a strongly silica-undersaturated series (normative ne > 13), of sodic character, consisting of basanites, tephrites, phonotephrites, and phonolites. Ar-Ar data for biotite from a tephriphonolite dike yielded an average age of 85.4±0.4 Ma. All three series show similar evolution trends controlled mainly by early-fractionation of olivine, spinel and clinopyroxene and, secondly, fractionation of plagioclase and apatite. Different degrees of crustal assimilation are suggested for each series. Assimilation processes are responsible for the wide 87 Sr/ 86 Sr i (0.70447-0.71172) and 143 Nd/ 144 Nd i (0.51237-0.51244) ranges, for the increased K 2 O/Na 2 O, Ba/Sr and Rb/Sr ratios, and for the reduced Ce/Pb ratios. In general, a significant contribution from partially melted host rocks is associated to the weakly silica-undersaturated potassic series. A phlogopite-bearing carbonate-metasomatized heterogeneous peridotite source accounts for the main chemical variations in the least contaminated samples of each series. Geochemical signatures of the strongly sodic alkaline series indicate a greater contribution from carbonated pyroxene-rich veins in the mantle source.

RESUMO A inserção das árvores em sistemas de integração lavoura, pecuária e floresta (ILPF) permite diversificar a produção, aumentar a rentabilidade por área e proporcionar maior conforto animal. Entretanto, a influência do componente arbóreo sobre os atributos químicos do solo neste sistema ainda é pouco estudada. Neste estudo, objetivou-se avaliar o efeito das árvores e das faces de exposição solar sobre a disponibilidade de nutrientes no solo em diferentes distâncias de renques de árvores em um sistema de integração lavoura pecuária floresta. O trabalho foi realizado no norte do estado do Mato Grosso, em região de transição Cerrado/ Floresta Amazônica em um sistema ILPF com o componente arbóreo formado por renques de três linhas de eucalipto. A avaliação efetuada no segundo ano de implantação do sistema ILPF considerou o efeito da distância das árvores (3, 6, 10 e 15 m) em duas faces de exposição solar (norte e sul) sobre os principais atributos químicos do solo. A amostragem do solo foi efetuada nas profundidades de 0-0,05; 0,05-0,10; 0,10-0,20 e 0,20-0,30 m. Os resultados indicaram homogeneidade dos teores médios de nutrientes obtidos nas faces norte e sul, bem como na comparação das mesmas distâncias nas duas faces de exposição avaliadas. Efeitos das árvores sobre os atributos químicos do solo foram verificados apenas quando da análise isolada na face norte para os teores de K e Al. Para esses atributos, o aumento da distância do renque foi associado à redução dos teores nas camadas mais superficiais e mais profundas, respectivamente. A disponibilidade de nutrientes no solo não é afetada significativamente pelas árvores nos primeiros anos de implantação do sistema ILPF em sistemas em que há homogeneidade na incidência de raios solares nas faces de exposição, ainda que possa reduções discretas de nutrientes com o distanciamento das árvores.

ABSTRACT The use of trees in integrated crop-livestock-forestry systems (ICLF) allows diversifying production, increasing profitability per area, and providing a higher animal comfort. However, the influence of tree component on soil chemical attributes in this system is still little studied. This study aimed to assess the effect of trees and sun-exposed faces to solar radiation on soil nutrient availability at different distances of tree rows in an integrated crop-livestock-forestry system. This study was carried out in the northern Mato Grosso State, Brazil, in the Cerrado - Amazon Forest transition region in an ICLF with the tree component formed by three eucalyptus rows. The assessment carried out in the second year of ICLF implantation considered the effect of tree distances (3, 6, 10, and 15 m) on two sun-exposed faces to solar radiation (north and south) on the main soil chemical attributes. Soil sampling was performed at depths of 0-0.05, 0.05-0.10, 0.10-0.20, and 0.20-0.30 m. The results indicated a homogeneity of the average nutrient contents obtained in the north and south-exposed faces, as well as in the comparison of the same distances in both assessed sun-exposed faces. Effects of trees on soil chemical attributes were verified only when an isolated analysis was carried out in the north-exposed face for K and Al contents. For these attributes, the increased distance in the row was associated with a reduction in their contents in the more superficial and deeper layers, respectively. Trees do not significantly affect soil nutrient availability in the first years of ICLF implantation in systems with homogeneity of solar radiation incidence in the sun-exposed faces although there may be discrete nutrient reductions with the distance of trees.

RESUMO: Conhecer a exigência hídrica de espécies florestais na fase de muda, além de proporcionar maior produtividade, possibilita reduzir custos, eliminando irrigações desnecessárias. Este estudo foi realizado com o objetivo de avaliar o efeito dos regimes hídricos no crescimento inicial, produção de folhas, teor e composição química do óleo essencial de Eucalyptus globulus , nos primeiros 120 dias de cultivo. O trabalho foi conduzido no Instituto de Ciências Agrárias da Universidade Federal de Minas Gerais (ICA/UFMG), Montes Claros, MG, empregando o delineamento inteiramente casualizado. Os tratamentos consistiram em seis regimes hídricos (50%, 75%, 100%, 125%, 150% e 175% da Evapotranspiração de referência - ETo), com quatro repetições. O crescimento das plantas foi avaliado por meio de dimensões lineares - altura, diâmetro do coleto e número de folhas. O óleo essencial foi extraído das folhas frescas e obtido pelo processo de hidrodestilação , em aparelho Clevenger. Neste estudo, foi observado que lâminas de irrigação correspondentes a 75% da ETo são responsáveis por maior crescimento do E. globulus e maior produção de matéria seca das folhas. As menores lâminas de irrigação foram responsáveis pelos maiores teores de óleo essencial. O óleo essencial foi analisado por cromatografia gasosa acoplada à espectrometria de massas (CG-EM), e apontou o eucaliptol (40,84 a 55,72%) como composto majoritário para todos os tratamentos. Os compostos como β-mirceno, α-Gurgujeno, Alloromadendreno e Varidiflorene apareceram em condições específicas de irrigação.

ABSTRACT: Understanding the water requirement of forest species in the seedling stage supports cost reduction by eliminating unnecessary irrigation in addition to providing higher productivity. This study was carried out to evaluate the effect of the water regime on initial growth, leaf production and content and chemical composition of the essential oil of Eucalyptus globulus , during the first 120 days of cultivation. The experiment employed a completely randomized design and was carried out at the Instituto de Ciências Agrárias da Universidade Federal de Minas Gerais (ICA/UFMG), Montes Claros, MG. Treatments consisted of six different water regimes (50%, 75%, 100%, 125%, 150% and 175% of the reference evapotranspiration - ETo) with four replicates. Plant growth was evaluated by measuring linear dimensions - height, stem diameter and number of leaves. The essential oil was extracted from fresh leaves by hydrodistillation in a Clevenger apparatus. The present study demonstrated that irrigation depths corresponding to 75% of the ETo are responsible for increased growth of E. globulus and increased dry matter production of leaves. The lowest irrigation levels were responsible for the greatest essential oil content. The essential oil was analyzed by gas chromatography-mass spectrometry (CG- MS), and eucalyptol (40.84% to 55.72%) was reported to be the major compound for all treatments. Compounds such as β-myrcene, α-Gurgujeno, Alloromadendreno, Varidiflorene appear under specific irrigation conditions.

RESUMO: A piometra é afecção frequente em cadelas e pode causar glomerulopatias e lesões tubulares. O presente estudo objetivou avaliar injúria renal em cadelas com piometra, sua progressão ao longo do tratamento e o uso de marcadores dessa alteração. Participaram 20 cadelas com diagnóstico clínico e ultrassonográfico de piometra, submetidas à fluidoterapia, antibioticoterapia e ovariohisterectomia. Foram avaliados pressão arterial, concentração sérica de creatinina, fósforo e ureia; urinálise e bioquímica urinária (gama-glutamiltransferase urinária e razão proteína/creatinina urinárias), taxa de filtração glomerular e débito urinário, em oito tempos. Todas as cadelas apresentaram algum grau de injúria renal no momento do diagnóstico da piometra, sendo transitória na maioria dos animais após o tratamento. O uso de marcadores de injúria renal identificou lesão de parênquima renal, contribuindo com o prognóstico dos animais estudados.

ABSTRACT: Pyometra is a common disease in intact female dogs and can cause glomerulopathy and tubular injury. This study aimed to evaluate kidney injury in female dogs with pyometra, as well as progression of the injury during treatment and the markers of this condition. This study analyzed 20 intact female dogs with both clinical and sonographic diagnosis of pyometra. Dogs were treated with intravenous fluids and antibiotics, and an ovariohysterectomy was performed. The following parameters were assessed at eight separate time points: blood pressure; serum creatinine, phosphorus, and urea levels; urinalysis and urinary biochemical parameters [urinary gamma-glutamyl transferase (uGGT) and urinary protein-to-creatinine ratio (UPCR)]; glomerular filtration rate (GFR); and urine output. All dogs showed some degree of kidney injury at the time of pyometra diagnosis. This was transient in most animals, resolving with treatment of the pyometra. Measurement of uGGT and UPCR identified renal parenchymal injury, helping to determine the prognosis of the animals analyzed in the present study.