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RESUMO: A crescente demanda por água de coco tem ocasionado a expansão do cultivo de coqueiro-anão-verde irrigado, no entanto, o uso ineficiente da água tem contribuído com a degradação e desperdício dos recursos hídricos, tornando fundamental estudos que visem otimizar o uso da água. Objetivou-se determinar a produtividade física e a produtividade da água do coqueiro-anão-verde, nas condições climáticas de Santa Izabel do Pará, leste da Amazônia, Brasil. O experimento foi realizado no período de 2020 a 2021, em um plantio de coqueiro-anão-verde em espaçamento 7,5 m x 7,5 m em triângulo, na Fazenda Reunidas Sococo, apresentando uma área de aproximadamente 7 ha. A produtividade da água (PA) foi determinada tanto em termos de produção de frutos (PAf) como de produção de água de coco (PAac), através da relação da produtividade de frutos (biomassa do fruto e volume de água) com a evapotranspiração acumulada. O coqueiro-anão apresentou uma produção total de 105 e de 186 frutos planta-1 e de 37 e de 62 L planta-1 de água de coco nos anos de 2020 e 2021. A evapotranspiração total foi de 56,2 m³ (2020) e 53,2 m-3 (2021). A PAf foi de 1,87 frutos m-3 (2020) e 2,94 frutos m-3 (2021) e a PAac de 0,66 L m-3 (2020) e 0,98 L m-3 (2021). RESUMO coqueiroanãoverde coqueiro anão verde irrigado entanto hídricos Objetivouse Objetivou se coqueiroanãoverde, verde, Pará Amazônia Brasil 202 2021 75 5 7, triângulo Sococo ha PA (PA (PAf PAac, , (PAac) biomassa acumulada coqueiroanão 10 18 planta1 planta 1 planta- 3 6 562 56 2 56, (2020 532 53 53, m3 m- . 187 87 1,8 294 94 2,9 (2021 066 0 66 0,6 098 98 0,9 20 (PAac (202 8 1, 29 9 2, 06 0, 09 (20 (2 (

ABSTRACT: The growing demand for coconut water has led to the expansion of irrigated green dwarf coconut cultivation; however, the inefficient use of water has contributed to the degradation and waste of water resources, making fundamental studies aiming to optimize the use of water. The objective of this study was to determine the physical productivity and water productivity of green dwarf coconut palms under the climatic conditions of Santa Izabel do Pará, eastern Amazonia, Brazil. The experiment was carried out from 2020 to 2021, in a green dwarf coconut plantation spaced 7.5 m x 7.5 m in a triangle, at Fazenda Reunidas Sococo, with an area of approximately 7 ha. Water productivity (WP) was determined both in terms of fruit production (WPf) and coconut water production (WPca), through the relationship of fruit productivity (fruit biomass and water volume) with accumulated evapotranspiration. The dwarf coconut tree presented a total production of 105 and 186 fruits plant-1 and 37 and 62 L plant-1 of coconut water in the years 2020 and 2021. The largest volumes of water and the highest weights occurred during the wettest season. WPf was of 1.87 fruits m-3 (2020) and 2.94 fruits m-3 (2021) and WPca 0.66 L m-3 (2020) and 0.98 L m-3 (2021). ABSTRACT cultivation however resources Pará Amazonia Brazil 202 2021 75 5 7. triangle Sococo ha WP (WP (WPf WPca, , (WPca) volume evapotranspiration 10 18 plant1 plant 1 plant- 3 6 season 187 87 1.8 m3 m- (2020 294 2 94 2.9 (2021 066 0 66 0.6 098 98 0.9 . 20 (WPca 8 1. (202 29 9 2. 06 0. 09 (20 (2 (

https://doi.org/10.1590/0103-8478cr20230416

Net radiation partitioning, evapotranspiration, and crop coefficients of the green dwarf coconut in Santa Izabel do Pará, Brazilian Amazon partitioning evapotranspiration Pará
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Carvalho, Erika de Oliveira Teixeira de

; Fernandes, Gabriel Siqueira Tavares

; Rua, Matheus Lima

; Monteiro, Adrielle Carvalho

; Luz, Denilson Barreto da

; Lisboa, Stefany Porcina Peniche

; Silva, João Vitor Ferreira da

; Pinto, João Vitor de Nóvoa

; Miranda, Fábio Rodrigues de

; Lins, Paulo Manoel Pontes

; Souza, Paulo Jorge de Oliveira Ponte de

Bragantia

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2024, Volume 83 elocation e20230160

Resumo: En | Texto: En | PDF: En

ABSTRACT Inadequate management of the irrigation system has compromised yield and favoured the degradation and waste of water resources. To ensure efficient irrigation management, providing yield increases, sustainability in the use of water resources and reduction of production costs, studies about the water demand of coconut palms are needed. The objective of this study was to determine the energy partition, crop evapotranspiration (ETc), and the simple crop coefficients (Kc) of the green dwarf coconut palms in Santa Izabel do Pará, Pará, Brazil. The experiment was carried out in a coconut plantation at Reunidas Sococo Farm, with an area of approximately 7 ha in a triangular spacing of 7.5 × 7.5 m, during 2020 and 2021. The ETc was determined by the Bowen ratio method, with measurements of temperature and relative humidity at two levels above the crop canopy and reference evapotranspiration (ETo) by the FAO Penman-Monteith method, using data from the National Institute of Meteorology. The Kc was determined by the ratio between ETc and ETo. The total water demand of the coconut palm was 489 (2020) and 480 mm (2021), with a daily average of 4.21 (2020) and 4.14 mm (2021) for the dry season. The mean value of Kc was 1.06 (± 0.12). Evapotranspiration was driven mainly by energy availability, associated with a control of atmospheric demand on Kc values, suggesting a possible influence of coconut trees on Kc values even under good water availability and small variability in vegetation cover. increases costs needed partition ETc, , (ETc) (Kc Pará Brazil Farm 75 5 7. m 202 2021 method ETo (ETo PenmanMonteith Penman Monteith Meteorology 48 (2020 2021, 421 4 21 4.2 414 14 4.1 (2021 season 106 1 06 1.0 ± ( 0.12. 012 0.12 . 0 12 0.12) cover (ETc 20 (202 42 2 4. 41 10 1. 01 0.1 (20 0. (2

https://doi.org/10.1590/1678-4499.20230160

Safety of CoronaVac and ChAdOx1 vaccines against SARS-CoV-2 in patients with rheumatoid arthritis: data from the Brazilian multicentric study safer ChAdOx SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- arthritis SARS-CoV
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Cruz, Vitor Alves

; Guimarães, Camila

; Rêgo, Jozelia

; Machado, Ketty Lysie Libardi Lira

; Miyamoto, Samira Tatiyama

; Burian, Ana Paula Neves

; Dias, Laiza Hombre

; Pretti, Flavia Zon

; Batista, Danielle Cristina Filgueira Alves

; Mill, José Geraldo

; Oliveira, Yasmin Gurtler Pinheiro de

; Gadelha, Carolina Strauss Estevez

; Gouveia, Maria da Penha Gomes

; Moulin, Anna Carolina Simões

; Souza, Bárbara Oliveira

; Aguiar, Laura Gonçalves Rodrigues

; Vieira, Gabriel Smith Sobral

; Grillo, Luiza Lorenzoni

; Lima, Marina Deorce de

; Pasti, Laís Pizzol

; Surlo, Heitor Filipe

; Faé, Filipe

; Moulaz, Isac Ribeiro

; Macabú, Mariana de Oliveira

; Ribeiro, Priscila Dias Cardoso

; Magalhães, Vanessa de Oliveira

; Aguiar, Mariana Freitas de

; Biegelmeyer, Erika

; Peixoto;, Flávia Maria Matos Melo Campos

; Kayser, Cristiane

; Souza, Alexandre Wagner Silva de

; Castro, Charlles Heldan de Moura

; Ribeiro, Sandra Lúcia Euzébio

; Telles, Camila Maria Paiva França

; Bühring, Juliana

; Lima, Raquel Lima de

; Santos, Sérgio Henrique Oliveira Dos

; Dias, Samuel Elias Basualto

; Melo, Natália Seixas de

; Sanches, Rosely Holanda da Silva

; Boechat, Antonio Luiz

; Sartori, Natália Sarzi

; Hax, Vanessa

; Dória, Lucas Denardi

; Rezende, Rodrigo Poubel Vieira de

; Baptista, Katia Lino

; Fortes, Natália Rodrigues Querido

; Melo, Ana Karla Guedes de

; Melo, Tâmara Santos

; Vieira, Rejane Maria Rodrigues de Abreu

; Vieira, Adah Sophia Rodrigues

; Kakehasi, Adriana Maria

; Tavares, Anna Carolina Faria Moreira Gomes

; Landa, Aline Teixeira de

; Costa, Pollyana Vitoria Thomaz da

; Azevedo, Valderilio Feijó

; Martins-Filho, Olindo Assis

; Peruhype-Magalhães, Vanessa

; Pinheiro, Marcelo de Medeiros

; Monticielo, Odirlei André

; Reis-neto, Edgard Torres Dos

; Ferreira, Gilda Aparecida

; Souza, Viviane Angelina de

; Teixeira-Carvalho, Andréa

; Xavier, Ricardo Machado

; Sato, Emilia Inoue

; Valim, Valeria

; Pileggi, Gecilmara Salviato

; Silva, Nilzio Antonio da

Advances in Rheumatology

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2024, Volume 64 elocation 58

Resumo: En | Texto: En | PDF: En

Abstract Background Patients with immune-mediated rheumatic diseases (IMRDs) have been prioritized for COVID-19 vaccination to mitigate the infection severity risks. Patients with rheumatoid arthritis (RA) are at a high risk of severe COVID-19 outcomes, especially those under immunosuppression or with associated comorbidities. However, few studies have assessed the safety of the COVID-19 vaccine in patients with RA. Objective To evaluate the safety of vaccines against SARS-CoV-2 in patients with RA. Methods This data are from the study “Safety and Efficacy on COVID-19 Vaccine in Rheumatic Diseases,” a Brazilian multicentric prospective phase IV study to evaluate COVID-19 vaccine in IMRDs in Brazil. Adverse events (AEs) in patients with RA of all centers were assessed after two doses of ChAdOx1 (Oxford/AstraZeneca) or CoronaVac (Sinovac/Butantan). Stratification of postvaccination AEs was performed using a diary, filled out daily and returned at the end of 28 days for each dose. Results A total of 188 patients with RA were include, 90% female. CoronaVac was used in 109 patients and ChAdOx1 in 79. Only mild AEs were observed, mainly after the first dose. The most common AEs after the first dose were pain at the injection (46,7%), headache (39,4%), arthralgia (39,4%), myalgia (30,5%) and fatigue (26,6%), and ChAdOx1 had a higher frequency of pain at the injection (66% vs 32 %, p < 0.001) arthralgia (62% vs 22%, p < 0.001) and myalgia (45% vs 20%, p < 0.001) compared to CoronaVac. The more common AEs after the second dose were pain at the injection (37%), arthralgia (31%), myalgia (23%), headache (21%) and fatigue (18%). Arthralgia (41,4% vs 25%, p = 0.02) and pain at injection (51,4% vs 27%, p = 0.001) were more common with ChAdOx1. No serious AEs were related. With Regard to RA activity level, no significant difference was observed between the three time periods for both COVID-19 vaccines. Conclusion In the comparison between the two immunizers in patients with RA, local reactions and musculoskeletal symptoms were more frequent with ChAdOx1 than with CoronaVac, especially after the first dose. In summary, the AE occurred mainly after the first dose, and were mild, like previous data from others immunizing agents in patients with rheumatoid arthritis. Vaccination did not worsen the degree of disease activity. immunemediated immune mediated (IMRDs COVID19 COVID 19 COVID-1 risks (RA outcomes comorbidities However SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- Safety Diseases, Diseases Brazil (AEs ChAdOx Oxford/AstraZeneca OxfordAstraZeneca Oxford AstraZeneca (Oxford/AstraZeneca Sinovac/Butantan. SinovacButantan Sinovac/Butantan . Sinovac Butantan (Sinovac/Butantan) diary 18 include 90 female 10 79 46,7%, 467 46,7% , 46 7 (46,7%) 39,4%, 394 39,4% 39 4 (39,4%) 30,5% 305 30 5 (30,5% 26,6%, 266 26,6% 26 6 (26,6%) 66% 66 (66 3 % 0.001 0001 0 001 62% 62 (62 22 22% 45% 45 (45 20 20% 37%, 37 37% (37%) 31%, 31 31% (31%) 23%, 23 23% (23%) 21% 21 (21% 18%. 18% (18%) 41,4% 414 41 (41,4 25 25% 0.02 002 02 51,4% 514 51 (51,4 27 27% related level summary COVID1 1 COVID- SARS-CoV (Sinovac/Butantan 9 46,7 (46,7% 39,4 (39,4% 30,5 (30,5 26,6 (26,6% (6 0.00 000 00 (4 (37% (31% (23% (21 (18% 41,4 (41, 0.0 51,4 (51, 46, (46,7 39, (39,4 30, (30, 26, (26,6 ( (37 (31 (23 (2 (18 41, (41 0. 51, (51 (46, (39, (30 (26, (3 (1 (5 (46 (39 (26

https://doi.org/10.1186/s42358-024-00397-5

Quantum computing: current and potential applications in digital agriculture computing
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Souza, Kleber Xavier Sampaio de

; Bolfe, Édson Luis

; Leite, Maria Angelica de Andrade

; Bambini, Martha Delphino

; Visoli, Marcos Cezar

; Luchiari Júnior, Ariovaldo

; da Silva, Felipe Rodrigues

; Esquerdo, Júlio César Dalla Mora

; Yassitepe, Juliana Erika de Carvalho Teixeira

Pesquisa Agropecuária Brasileira

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2024, Volume 59 elocation e03753

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Os computadores quânticos usam as propriedades da física quântica para realizar operações de armazenamento e processamento de informações. A operação desses computadores envolve conceitos como emaranhamento e superposição, que os dotam de grande poder de processamento que supera até mesmo o dos mais poderosos supercomputadores atuais, consumindo quantidades significativamente menores de energia. Os diferentes estudos analisados neste artigo de revisão sugerem que a computação quântica terá impacto profundo em áreas como finanças, logística, transporte, tecnologia espacial e automotiva, ciência dos materiais, energia, indústria farmacêutica e de saúde, segurança cibernética e agricultura. Na agricultura digital, foram identificadas e exemplificadas várias aplicações que poderiam ser executadas de forma mais eficiente em computadores quânticos para processamento de dados e compreensão de processos biológicos. Essas aplicações são agrupadas aqui nas quatro seguintes áreas: bioinformática, sensoriamento remoto, modelagem climática e agricultura inteligente. Este artigo também explora a importância estratégica do domínio da computação quântica, destaca algumas vantagens em relação à computação clássica e apresenta um mapeamento dos serviços já disponíveis, o que permitirá que instituições empreendam um planejamento estratégico para incorporar a computação quântica em seus processos de desenvolvimento. Por fim, são destacados os desafios para a implementação da computação quântica, juntamente com algumas iniciativas em andamento que visam aprofundar a pesquisa na vanguarda do conhecimento nesta área aplicada à agricultura digital. informações superposição atuais energia finanças logística transporte automotiva materiais saúde digital biológicos bioinformática remoto inteligente disponíveis desenvolvimento fim

Abstract Quantum computers use the properties of quantum physics to perform information storage and processing operations. The operation of these computers involves concepts such as entanglement and superposition, which endow them with a great processing power that even surpasses that of the most powerful current supercomputers, while consuming significantly lower amounts of energy. The different studies analyzed in this review article suggest that quantum computing will have a deep impact in areas such as finance, logistics, transportation, space and automotive technology, materials science, energy, pharmaceutical and healthcare industry, cybersecurity, and agriculture. In digital agriculture, several applications that could be executed more efficiently in quantum computers for data processing and understanding of biological processes were identified and exemplified. These applications are grouped here into the following four areas: bioinformatics, remote sensing, climate modeling, and smart farming. This article also explores the strategic importance of mastering quantum computing, highlights some advantages in relation to classical computing, and presents a mapping of the services already available, enabling institutions to undertake strategic planning for the incorporation of quantum computing into their development processes. Finally, the challenges for the implementation of quantum computing are highlighted, along with some ongoing initiatives aimed at furthering research at the forefront of knowledge in this area applied to digital agriculture. operations superposition supercomputers energy finance logistics transportation technology science industry cybersecurity agriculture exemplified bioinformatics sensing modeling farming available Finally highlighted

https://doi.org/10.1590/s1678-3921.pab2024.v59.03753

[SciELO Preprints] - Initial evaluations of the use of eugenol as an antioxidant in the in vitro maturation of ovine oocytes
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Santos, Sara Rodrigues Pereira dos Souza, Brenna Maria Silva de Silva, Bruna Moura Santos, Damaris Raquel Pires dos Aureliano, Érika Karoline de Oliveira Lima, Andreza Mayara Carneiro Oliveira, Luana Kealy Pimentel de Lima, Sueli de Oliveira Pereira , Ailton Batista Lopes, Isabela Maria Freitas, Beatriz Cavalcanti de Souza, Thais Thatiane dos Santos Sousa, Pedro Humberto Félix de Cordeiro, Mabel Freitas Lopes Júnior, Edilson Soares

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Data de publicação: 2023-12-22

Resumo: En Es Pt

The aim of this study was to assess the effect of eugenol on the in vitro production of ovine embryos. Oocytes were aspirated and taken for in vitro maturation (IVM), where they were divided into 4 groups: the CON (control), in which the oocytes were immersed in medium without an antioxidant; in the EU10, EU20, EU40 groups, the same medium as in the COM group was used, supplemented with 10, 20, and 40 µM of eugenol, respectively. The degree of expansion of cumulus cells was evaluated. Subsequently, the oocytes underwent in vitro fertilization and in vitro culture, and at the end of this process, the rate of cleaved structures was assessed. Regarding the expansion rate of cumulus cells, the CON group exhibited higher rates compared to the groups supplemented with eugenol. Evaluating oocytes with fully expanded cumulus cells, only the EU40 group showed a lower rate when compared to the rest of the treatment groups. Whereas observing oocytes with partially expanded cumulus cells, the EU40 group showed higher expansion percentages compared to the other treatments. Concerning the presence of the first polar body in the oocytes, it was observed that there was no statistical difference between the treatment groups. Lastly, concerning the cleaved structures, the CON and EU10 groups showed the highest rates compared to the EU20 and EU40 groups. Thus, it was possible to conclude that the use of 10 µM of eugenol in the IVM of ovine oocytes proved to be the optimal concentration of this substance.

El objetivo del presente estudio fue evaluar el efecto del eugenol en la producción in vitro de embriones ovinos. Los ovocitos fueron aspirados y llevados para la maduración in vitro (MIV), donde se dividieron en 4 grupos: el CON (control), en el cual los ovocitos fueron sumergidos en medio sin antioxidantes; en los grupos EU10, EU20, EU40 se utilizó el mismo medio que en el grupo CON, pero con la adición de 10, 20 y 40 µM de eugenol, respectivamente. Se evaluó el grado de expansión de las células del cúmulo. Después de esto, los ovocitos fueron sometidos a la fecundación in vitro y al cultivo in vitro, y al final de este proceso se evaluó la tasa de estructuras clivadas. En cuanto a la tasa de expansión de las células del cúmulo, el grupo CON mostró tasas más altas en comparación con los grupos suplementados con eugenol. Al evaluar los ovocitos con células del cúmulo totalmente expandidas, solo el grupo EU40 mostró una tasa más baja en comparación con el resto de los grupos de tratamiento. Mientras que al observar los ovocitos con células del cúmulo parcialmente expandidas, el grupo EU40 mostró porcentajes de expansión más altos en comparación con los otros tratamientos. Respecto a la presencia del primer corpúsculo polar en los ovocitos, se observó que no hubo diferencia estadística entre los grupos de tratamiento. Por último, en relación con las estructuras clivadas, los grupos CON y EU10 mostraron las tasas más altas en comparación con los grupos EU20 y EU40. Por lo tanto, se pudo concluir que el uso de 10 µM de eugenol en la MIV de ovocitos ovinos resultó ser la concentración óptima de esta sustância.

Objetivando avaliar o efeito do eugenol na produção in vitro de embriões ovinos. Os oócitos, foram aspirados e levados para a maturação in vitro (MIV), onde foram divididos em 4 grupos: o CON (controle), no qual os oócitos foram imersos em meio, sem antioxidante, já nos grupos EU10, EU20, EU40 foi utilizado o mesmo meio do grupo CON, acrescido de 10, 20 e 40 µM de eugenol, respectivamente. Foi avaliado o grau de expansão das células do cumulus. Após isso, os oócitos seguiram para a fecundação in vitro e cultivo in vitro, sendo, ao final desse processo, avaliada a taxa de estruturas clivadas. Em relação à taxa de expansão das células do cumulus, o grupo CON apresentou maiores taxas, quando comparado aos grupos adicionados de eugenol. Avaliando os oócitos com células do cumulus totalmente expandidas, somente o grupo EU40, apresentou-se menor, quando comparado ao restante dos grupos de tratamento. Já observando os oócitos com células do cumulus parcialmente expandidas, o grupo EU40, apresentou maiores percentuais de expansão, quando comparado aos demais tratamentos. Sobre a presença do primeiro corpúsculo polar nos oócitos, observou-se que não houve diferença estatística entre os grupos de tratamentos. E, por último, em relação às estruturas clivadas, os grupos CON e EU10 apresentaram as maiores taxas, quando comparado aos grupos EU20 e EU40. Dessa maneira, foi possível concluir que a utilização de 10 µM de eugenol na MIV de oócitos ovinos apresentou ser a melhor concentração dessa substância.

https://doi.org/10.1590/SciELOPreprints.7755

[SciELO Preprints] - Initial evaluations of quercetin on the in vitro maturation of ovine oocytes
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Silva, Bruna Moura Santos, Sara Rodrigues Pereira dos Souza, Brenna Maria Silva de Santos, Damaris Raquel Pires dos Aureliano, Érika Karoline de Oliveira Lima, Andreza Mayara Carneiro Oliveira, Luana Kealy Pimentel de Lima, Sueli de Oliveira Pereira , Ailton Batista Lopes, Isabela Maria Freitas, Beatriz Cavalcanti de Souza, Thais Thatiane dos Santos Sousa, Pedro Humberto Félix de Cordeiro, Mabel Freitas Lopes Júnior, Edilson Soares

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Data de publicação: 2023-12-22

Resumo: En Es Pt

The aim of this study was to evaluate the effect of quercetin on the in vitro maturation (IVM) of oocytes and the subsequent in vitro fertilization (IVF) of ovine embryos. For this purpose, oocytes were collected and divided into the following treatment groups: control group, containing CON medium (control); and groups Q2, Q4, and Q8, with control medium supplemented with 2, 4, and 8 µM of quercetin, respectively. After IVM, the degree of cumulus cells expansion, presence of the first polar body (PB), and the other part of the oocytes proceeded to in vitro fertilization (IVF), where oocytes were incubated with spermatozoa for a period of 20 hours. Subsequently, the presumptive zygotes underwent in vitro culture (IVC) for 48 hours, and at the end of the process, the rate of cleaved structures was evaluated. The results were expressed in percentage and compared using the Chi-square test, with a significant difference considered when P<0.05. Evaluating the expansion of cumulus cells, the CON and Q2 groups showed the best expansion rates compared to the Q4 and Q8 groups. Regarding the presence of the 1st PB, it was observed that only the Q8 group exhibited lower rates compared to the control group. When evaluating the rate of cleaved structures, it was observed that the Q2 group showed a higher number compared to the Q8 group. Quercetin does not influence the maturation of ovine oocytes.

El objetivo del presente estudio fue evaluar el efecto de la quercetina en la maduración in vitro (MIV) de ovocitos y la posterior fertilización in vitro (FIV) de embriones ovinos. Para ello, se recolectaron y seleccionaron ovocitos divididos en los siguientes grupos de tratamiento: grupo control, que contenía medio CON (control); y los grupos Q2, Q4 y Q8, con medio control al que se le añadieron 2, 4 y 8 µM de quercetina, respectivamente. Tras la MIV se evaluó el grado de expansión de las células del cúmulo, la presencia del primer corpúsculo polar (CP) y la otra parte de los ovocitos se utilizó para la fecundación in vitro (FIV), donde los ovocitos fueron incubados con espermatozoides durante un período de 20 horas. Posteriormente, los presuntos cigotos pasaron a cultivo in vitro (CIV) durante 48 horas y, al final del proceso, se evaluó la tasa de estructuras clivadas. Los resultados se expresaron en porcentaje y se compararon utilizando la prueba de Chi-cuadrado, considerando una diferencia significativa cuando P<0,05. Al evaluar la expansión de las células del cúmulo, los grupos CON y Q2 mostraron las mejores tasas de expansión en comparación con los grupos Q4 y Q8. En cuanto a la presencia del 1er CP, se observó que solo el grupo Q8 mostró tasas más bajas en comparación con el grupo control. Al evaluar la tasa de estructuras clivadas, se observó que el grupo Q2 mostró un número mayor en comparación con el grupo Q8. La quercetina no influye en la maduración de ovocitos ovinos.

O objetivo do presente estudo foi avaliar o efeito da quercetina na MIV de oócitos e na subsequente PIV de embriões ovinos. Para isso, foram colhidos e selecionados oócitos divididos nos seguintes grupos de tratamento: grupo controle, contendo meio CON (controle); e grupos Q2, Q4 e Q8, com meio controle, acrescido de 2, 4 e 8 µM de quercetina, respectivamente. Após a MIV foi avaliado o grau de expansão das células do cumulus, presença do primeiro corpúsculo polar (CP) e a outra parte dos oócitos seguiu para a fecundação in vitro (FIV), onde os oócitos foram incubados com os espermatozoides, por um período de 20 h. Em seguida, os presumíveis zigotos seguiram para o cultivo in vitro (CIV), por um período de 48 h e, ao final do processo, foi avaliada a taxa de estruturas clivadas. Os resultados foram expressos em porcentagem e comparados, usando o Teste do Qui-quadrado e foi considerada diferença significativa quando P<0,05. Avaliando a expansão das células do cumulus, os grupos CON e Q2 apresentaram as melhores taxas de expansão, quando comparadas aos grupos Q4 e Q8. Quanto à presença do 1º CP, foi possível observar que somente o grupo Q8 apresentou taxas menores, quando comparado ao grupo controle. Já avaliando a taxa de estruturas clivadas, foi possível observar que o grupo Q2 apresentou maior número quando comparado ao grupo Q8. A quercetina não influencia na maturação de oócitos ovinos.

https://doi.org/10.1590/SciELOPreprints.7759

Full confluency, serum starvation, and roscovitine for inducing arrest in the G0/G1 phase of the cell cycle in puma skin-derived fibroblast lines confluency starvation G0G1 GG G0 G1 G G0/G skinderived skin derived G0G
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Rodrigues, Luanna Lorenna Vieira

; Moura, Yasmin Beatriz França

; Viana, João Vitor da Silva

; Oliveira, Lhara Ricarliany Medeiros de

; Praxedes, Érika Almeida

; Vieira Neto, José de Brito

; Sales, Sarah Leyenne Alves

; Silva, Herlon Victor Rodrigues

; Luciano, Maria Claudia dos Santos

; Pessoa, Claudia

; Pereira, Alexsandra Fernandes

Animal Reproduction

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2023, Volume 20 Nº 1 elocation e20230017

Resumo: En | Texto: En | PDF: En

Abstract The puma population is constantly decreasing, and cloning by somatic cell nuclear transfer can be used to conserve the species. One of the factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We evaluated the effects of full confluency (~100%), serum starvation (0.5% serum), and roscovitine (15 µM) treatments on the cell cycle synchronization in G0/G1 of puma skin-derived fibroblasts by flow cytometric analysis. Also, we assessed the effects of these synchronization methods on morphology, viability, and apoptosis levels using microscopy tools. The results showed that culturing the cells to confluence for 24 h (84.0%), 48 h (84.6%), and 72 h (84.2%) and serum starvation for 96 h (85.4%) yielded a significantly higher percentage of cells arrested in the G0/G1 (P < 0.05) phase than cells not subjected to any cell cycle synchronization method (73.9%). Nevertheless, while serum starvation reduced the percentage of viable cells, no difference was observed for the full confluence and roscovitine treatments (P > 0.05). Moreover, roscovitine for 12 h (78.6%) and 24 h (82.1%) was unable to synchronize cells in G0/G1 (P > 0.05). In summary, full confluency induces puma fibroblast cell cycle synchronization at the G0/G1 stage without affecting cell viability. These outcomes may be valuable for planning donor cells for somatic cell nuclear transfer in pumas. decreasing species ~100%, 100 ~100% , (~100%) 0.5% 05 0 5 (0.5 serum, serum) 15 (1 µM G0G1 GG G0 G1 G G0/G skinderived skin derived analysis Also morphology viability tools 2 84.0%, 840 84.0% 84 (84.0%) 4 84.6%, 846 84.6% 6 (84.6%) 7 84.2% 842 (84.2% 9 85.4% 854 85 (85.4% P 0.05 005 73.9%. 739 73.9% . 73 (73.9%) Nevertheless 0.05. Moreover 1 78.6% 786 78 (78.6% 82.1% 821 82 (82.1% summary pumas 10 ~100 (~100% 0.5 (0. ( G0G 84.0 8 (84.0% 84.6 (84.6% 84.2 (84.2 85.4 (85.4 0.0 00 73.9 (73.9% 78.6 (78.6 82.1 (82.1 ~10 (~100 0. (0 84. (84.0 (84.6 (84. 85. (85. 73. (73.9 78. (78. 82. (82. ~1 (~10 (84 (85 (73. (78 (82 ~ (~1 (8 (73 (7 (~

https://doi.org/10.1590/1984-3143-ar2023-0017

Comments on “Assessment of pain and quality of life in patients undergoing cardiac surgery: a cohort study” Assessment surgery study
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Pontes-Silva, André

; Lopes, André Luiz

; Maciel, Erika da Silva

; Quaresma, Fernando Rodrigues Peixoto

; Santos-de-Araújo, Aldair Darlan

Revista da Associação Médica Brasileira

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2023, Volume 69 Nº 7 elocation e20230304

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1590/1806-9282.20230304

10.

Comments on “Evaluation of functional parameters of the foot and ankle in elderly with sarcopenia” Evaluation sarcopenia
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Anselmo-e-Silva, Cassius Iury

; Santos, Leticia Menegalli

; Lopes, André Luiz

; Maciel, Erika da Silva

; Quaresma, Fernando Rodrigues Peixoto

; Santos-de-Araújo, Aldair Darlan

; Pontes-Silva, André

Revista da Associação Médica Brasileira

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2023, Volume 69 Nº 8 elocation e20230583

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1590/1806-9282.20230583

11.

Brazilian pulmonology guidelines on Delphi panel for post-coronavirus disease 2019: reflections on persons deprived of liberty postcoronavirus post coronavirus 2019 201 20 2
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Bucar, Sarah Maria Reis

; Lima, Thiago Oliveira Sabino de

; Silva, Gislaine Aneanes da

; Maciel, Erika da Silva

; Pontes-Silva, André

; Quaresma, Fernando Rodrigues Peixoto

Revista da Associação Médica Brasileira

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2023, Volume 69 Nº 11 elocation e20230965

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1590/1806-9282.20230965

12.

Psychometric properties of the online Satisfaction with Life Scale in university students from a low-income region lowincome low income
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Cardoso, Antonio Gibran de Almeida

; Carvalho, Millena Vaz de

; Silva, Maria Isabela Alves de Almeida

; Franco, Alaiana Marinho

; Quaresma, Fernando Rodrigues Peixoto

; Maciel, Erika Da Silva

; Nascimento-Ferreira, Marcus Vinicius

Psicologia: Reflexão e Crítica

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2023, Volume 36 elocation 12

Resumo: En | Texto: En | PDF: En

Abstract Purpose To test the feasibility, reliability, and validity of the Satisfaction with Life Scale (SWLS) in an online format in university students from a low-income region. Methods This was a psychometric study, involving a study of reliability ( n = 117) and validity ( n = 195) in university students from a region with a Gini index of 0.56. The scale was applied at two time points with an interval of 2 weeks. This scale measures satisfaction with life based on five statements and responses ranging from 1 to 7 (strongly disagree to strongly agree). We conducted the reliability assessment using temporal stability and internal consistency and construct validity assessment by internal structure solution. Results All SWLS items showed acceptable (rho > 0.30) and significant ( p < 0.05) temporal stability and acceptable internal consistency (alpha > 0.70). In construct validity (internal structure), we identified a factor with an explained variance of 59.0% in the exploratory factor analysis. Additionally, in the confirmatory factor analysis, we identified a one-factor structure solution for SWLS with an acceptable model fitting (chi-square/degrees of freedom [ X 2/df] = 6.53; Tucker–Lewis Index [TLI] = 0.991; Comparative Fit Index [CFI] = 0.996; root mean square error of approximation [RMSEA] = 0.040; standardized root mean-squared residual [SRMR] = 0.026). Conclusion The Satisfaction with Life Scale, in the online format, is a reliable and valid tool for university students in a low-income context. feasibility (SWLS lowincome low income 117 195 056 0 56 0.56 weeks agree. agree . agree) rho 0.30 030 30 0.05 005 05 alpha 0.70. 070 0.70 70 0.70) structure, , structure) 590 59 59.0 analysis Additionally onefactor one chisquare/degrees chisquaredegrees chi square/degrees degrees 2/df 2df df 6.53 653 6 53 TuckerLewis Tucker Lewis TLI [TLI 0.991 0991 991 CFI [CFI 0.996 0996 996 RMSEA [RMSEA 0.040 0040 040 meansquared squared SRMR [SRMR 0.026. 0026 0.026 026 0.026) context 11 19 5 0.5 0.3 03 3 0.0 00 07 0.7 59. chisquare squaredegrees 6.5 65 0.99 099 99 0.04 004 04 002 0.02 02 0. 6. 0.9 09 9

https://doi.org/10.1186/s41155-023-00254-2

13.

Profile of oropharyngeal dysphagia patients in a teaching hospital in Northern Brazil: a descriptive cross-sectional study Brazil crosssectional cross sectional
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Gonçalves, Ives Marcelo Pinheiro

; Pontes-Silva, André

; Zica, Matheus Morbeck

; Barasuol, Aldair Martins

; Maciel, Erika da Silva

; Quaresma, Fernando Rodrigues Peixoto

Revista da Associação Médica Brasileira

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2023, Volume 69 Nº 10 elocation e20230611

Resumo: En | Texto: En | PDF: En

SUMMARY OBJECTIVE: The aim of this study was to describe the profile of patients with oropharyngeal dysphagia in a teaching hospital in the public health system in northern Brazil. METHODS: This is a descriptive cross-sectional study. All procedures of this study were approved by the ethics committee. A convenience sample composed of participants aged >18 years, of both sexes, with any underlying pathology admitted to the medical clinic on exclusive oral feeding, alternatively enteral or gastric tube feeding (Gastrostomy), or associated by both routes, whose swallowing assessment was performed by a Speech-Language Pathologist. Data from the database/medical records were investigated from March 2020 to September 2021. RESULTS: The sample consisted of 44 patients diagnosed with oropharyngeal dysphagia, with a higher frequency of males (63.64%) aged over 60 years (70.45%). Almost half of the evaluated patients were diagnosed with neurological disorders (47.73%) and had dysphagia associated with other underlying diseases (31.82%). Excluding patients with neurological disorders, trauma/polytrauma, and respiratory disorders from the last group, some patients (11.36%) had two concomitant underlying diseases. CONCLUSION: According to the sample of this study, the profile of oropharyngeal dysphagia patients includes pneumonia, respiratory failure, bronchoaspiration, and the consequent need for ventilatory support. OBJECTIVE Brazil METHODS crosssectional cross sectional committee 18 >1 sexes Gastrostomy, Gastrostomy , (Gastrostomy) routes SpeechLanguage Speech Language Pathologist databasemedical database 202 2021 RESULTS 4 63.64% 6364 63 64 (63.64% 6 70.45%. 7045 70.45% . 70 45 (70.45%) 47.73% 4773 47 73 (47.73% 31.82%. 3182 31.82% 31 82 (31.82%) traumapolytrauma trauma polytrauma trauma/polytrauma group 11.36% 1136 11 36 (11.36% CONCLUSION pneumonia failure bronchoaspiration support 1 > (Gastrostomy 20 63.64 636 (63.64 704 70.45 7 (70.45% 47.73 477 (47.73 318 31.82 3 8 (31.82% 11.36 113 (11.36 2 63.6 (63.6 70.4 (70.45 47.7 (47.7 31.8 (31.82 11.3 (11.3 63. (63. 70. (70.4 47. (47. 31. (31.8 11. (11. (63 (70. (47 (31. (11 (6 (70 (4 (31 (1 ( (7 (3

https://doi.org/10.1590/1806-9282.20230611

14.

Genome editing in maize: Toward improving complex traits in a global crop maize
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Hernandes-Lopes, José

; Yassitepe, Juliana Erika de Carvalho Teixeira

; Koltun, Alessandra

; Pauwels, Laurens

; Silva, Viviane Cristina Heinzen da

; Dante, Ricardo Augusto

; Gerhardt, Isabel Rodrigues

; Arruda, Paulo

Genetics and Molecular Biology

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2023, Volume 46 Nº 1 elocation e20220217

Resumo: En | Texto: En | PDF: En

Abstract Recent advances in genome editing have enormously enhanced the effort to develop biotechnology crops for more sustainable food production. CRISPR/Cas, the most versatile genome-editing tool, has shown the potential to create genome modifications that range from gene knockout and gene expression pattern modulations to allele-specific changes in order to design superior genotypes harboring multiple improved agronomic traits. However, a frequent bottleneck is the delivery of CRISPR/Cas to crops that are less amenable to transformation and regeneration. Several technologies have recently been proposed to overcome transformation recalcitrance, including HI-Edit/IMGE and ectopic/transient expression of genes encoding morphogenic regulators. These technologies allow the eroding of the barriers that make crops inaccessible for genome editing. In this review, we discuss the advances in genome editing in crops with a particular focus on the use of technologies to improve complex traits such as water use efficiency, drought stress, and yield in maize. production CRISPRCas CRISPR Cas genomeediting tool allelespecific allele specific However regeneration recalcitrance HIEdit/IMGE HIEditIMGE HI Edit/IMGE Edit IMGE ectopictransient ectopic transient regulators review efficiency stress maize HIEdit EditIMGE

https://doi.org/10.1590/1678-4685-gmb-2022-0217

15.

Prêmio APS Forte no Sistema Único de Saúde-Brasil: principais resultados e lições aprendidas SaúdeBrasil Saúde Brasil Saúde-Brasil
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Almeida, Erika Rodrigues de

; Pereira, Francy Webster de Andrade

; Silva, Michelle Leite da

Saúde em Debate

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Dez 2022, Volume 46 Nº spe8 Páginas 106 - 117

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Este trabalho apresenta uma análise dos principais resultados do Prêmio APS Forte no Sistema Único de Saúde (SUS), em suas três edições, por meio de análise documental e de dados secundários. Nas edições realizadas em 2019, 2020 e 2021, foram aprovadas 3.861 experiências, e premiadas 26 iniciativas de todo o País. Na primeira edição, com foco no acesso universal, foram destacadas experiências de reorganização do processo de trabalho na Atenção Primária à Saúde (APS), de modo a superar barreiras de acesso organizacional e/ou assistencial. Na segunda edição, realizada no contexto da pandemia da Covid-19, evidenciou-se o papel protagonista da APS no enfrentamento da Covid-19, com ações de prevenção, atenção, vigilância e coordenação do cuidado. A terceira edição suscitou a necessidade de práticas de cuidado multiprofissional ampliado para o alcance da integralidade e resolubilidade da APS. O Prêmio tem se configurado como importante estratégia de mobilização dos territórios, de educação permanente, de reconhecimento e compartilhamento de boas práticas e de cooperação institucional entre a Opas/OMS e o Ministério da Saúde do Brasil. A manutenção e a ampliação do Prêmio se mostram fundamental para o fortalecimento da APS e como iniciativa para construção e consolidação de laboratórios de inovação da APS no sistema de saúde brasileiro. SUS, SUS , (SUS) secundários 2019 202 2021 3861 3 861 3.86 2 País universal APS, (APS) eou ou assistencial Covid19, Covid19 Covid 19, 19 Covid-19 evidenciouse evidenciou prevenção atenção territórios permanente OpasOMS Opas OMS Brasil brasileiro (SUS 201 20 386 86 3.8 (APS Covid1 1 Covid-1 38 8 3. Covid-

ABSTRACT This work presents an analysis of the main results of the ‘APS Forte’ Award in the Unified Health System (SUS), in its three editions, through document analysis and secondary data. In all editions, held in 2019, 2020, and 2021, 3,861 experiences were approved and 26 initiatives from all over the country were awarded. In the first edition, focusing on universal access, experiences of reorganization of the work process in Primary Health Care (PHC) were highlighted, in order to overcome barriers to organizational and/or care access. In the second edition, held in the context of the COVID-19 pandemic, the protagonist role of PHC in the fight against COVID-19 was highlighted, with actions of prevention, attention, surveillance and coordination of care. The third edition raised the need for expanded multiprofessional care practices to achieve PHC comprehensiveness and resolution. The Award has been configured as an important strategy for mobilizing territories, for continuing education, for recognizing and sharing good practices, and for institutional cooperation between PAHO/WHO and the Ministry of Health of Brazil. The maintenance and expansion of the Award is fundamental for the strengthening of PHC and as an initiative for the construction and consolidation of PHC innovation laboratories in the Brazilian health system. APS Forte SUS, SUS , (SUS) editions data 2019 2020 2021 3861 3 861 3,86 2 awarded access (PHC highlighted andor or COVID19 COVID 19 COVID-1 pandemic prevention attention resolution territories education PAHOWHO PAHO WHO Brazil system (SUS 201 202 386 86 3,8 COVID1 1 COVID- 20 38 8 3,

https://doi.org/10.1590/0103-11042022e808

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