ABSTRACT The objective of this study was to determine the metabolizable energy, standardized ileal amino acid digestibility (SIAAD) and digestible amino acid values of wheat meal (WM), wheat gluten meal (WGM), meat bone meal (MBM) and poultry-by-product meal (PPM) in diets for broilers. In the first experiment, the total excreta collection method was used, with 180 chicks with 14 days, distributed in a completely randomized design, with 4 feeds + reference diet with 6 replicates and 6 broilers each. In the second experiment, 180 broilers from 24 to 28 days old were used, distributed in a completely randomized design, with 4 feeds + one protein free diet with 6 replicates and 6 broilers each. The AMEn values of WM, WGM, MBM and PPM were 3052, 3174, 1556 and 3200 (kcal/kg), respectively. The average SIAAD of the essential and non-essential amino acids of WM, WGM, MBM and PPM were 74.21, 97.50, 69.28 and 83.08; 76.32, 96.42, 63.93 and 77.66, respectively. The values of the main digestible limiting amino acids were 0.18, 1.42, 0.41 and 0.94 for methionine; 0.20, 1.48, 1.73 and 2.73 for lysine; 0.23, 1.67, 0.72 and 1.89 for threonine; and 0.35, 3.22, 1.38 and 2.47 for valine, respectively for the feedstuffs in study.

The objective of this study was to verify the agreement between pre-programmed and executed pacing during race walking and whether level of the athletes experience and performance influenced this relationship. Twenty-nine national and international race walkers participated in this study (14 males, 24.0±7.1 years old, and 15 females, 23.3±7.3 years old). Pre-programmed pacing for 10- and 20-km official walking races was self-selected via demonstrative pacing charts prior to races, while executed pacing was analyzed by a specialist investigator via an individual plot of current velocity versus distance. There was no agreement between pre-programmed and executed pacing (P=0.674). There was no association between the ability to match the pre-programmed pace with the executed pace and race walking experience or level of performance. Low- and high-performance athletes pre-programmed a similar pacing profile (P=0.635); however, high-performance athletes generally executed an even pacing strategy, while low-performance athletes generally adopted a positive pacing strategy (P=0.013). Race walkers did not faithfully match their pre-programmed with their executed pacing, and this seemed to be independent of previous experience and level of performance. High-performance athletes, however, tended to execute an even pacing strategy, even though this had not been pre-programmed.

Abstract Objectives: The aim of the present study was to analyze and correlate muscle strength of the back and front squat with performance in the movements of weightlifting snatch and the clean in CrossFit® practitioners. Method: Twenty-two adults participated in this study and were divided according to their performance in the snatch and clean movements: stronger (29.6 ± 4.4 years; 80.95 ± 9.57 kg; 12.2 ± 5.8% body fat; 2.4 ± 0.9 training experience) and weaker (28.5 ± 5.4 years; 79.4 ± 6.8 kg; 13.6 ± 3.5% body fat; 2.0 ± 1.1 training experience). All volunteers completed one-repetition maximum tests, in separate days, in the following exercises: back squat, front squat, snatch and the first phase of the clean. Results: The volunteers classified with a superior performance in the snatch and clean presented a higher strength in the back squat (154.9 ± 20.3 kg vs 132.7 ± 11.6 kg; p = 0.009) and front squat (139.0 ± 14.5 kg vs 116.8 ± 11.3 kg; p = 0.002) as compared with the weaker volunteers. Additionally, there were strong correlations between relative strength in the back squat (r = 0.83; r = 0.76; p = 0.001) and front squat (r = 0.73; r = 0.83; p = 0.001) with the performance in the snatch and clean exercises, respectively. Conclusions: Therefore, a superior strength in basic exercises, such as back and front squat can contribute to weightlifting performance in CrossFit® practitioners.

Resumo Objetivos: O objetivo do presente estudo foi analisar e relacionar a força muscular do back squat e front squat com o desempenho nos movimentos de levantamento olímpico snatch e clean em praticantes de CrossFit®. Método: Participaram deste estudo 22 adultos, que foram divididos de acordo com o desempenho nos movimentos de snatch e clean: fortes (29.6 ± 4.4 anos; 80.95 ± 9.57 kg; 12.2 ± 5.8% de gordura; 2.4 ± 0.9 experiência de treinamento) e fracos (28.5 ± 5.4 anos; 79.4 ± 6.8 kg; 13.6 ± 3.5% de gordura; 2.0 ± 1.1 experiência de treinamento). Todos os voluntários realizaram em dias separados os testes de uma repetição máxima nos exercícios: back squat, front squat, snatch e clean. Resultados: Os voluntários classificados com o melhor desempenho no snatch e clean apresentaram maior força no back squat (154.9 ± 20.3 kg vs. 132.7 ± 11.6 kg; p = 0.009) e no front squat(139.0 ± 14.5 kg vs. 116.8 ± 11.3 kg; p = 0.002), quando comparados aos voluntários mais fracos. Além disso, foram observadas correlações fortes entre a força relativa do back squat (r = 0.83; r = 0.76; p = 0.001) e do front squat (r = 0.73; r = 0.83; p = 0.001) com a performance nos exercícios snatch e clean, respectivamente. Conclusões: Portanto, uma força superior em exercícios básicos, como o back squat e o front squat, podem contribuir para o desempenho do levantamento olímpico em praticantes de CrossFit®.

Resumen Objetivo: El propósito del presente estudio fue analizar y relacionar la fuerza muscular de la sentadilla por delante y por detrás con el rendimiento en los movimientos de halterofilia de arranque y de cargada en practicantes de CrossFit®. Método: Participaron en este estudio 22 adultos que fueron divididos de acuerdo con el rendimiento en los movimientos de arranque y cargada: fuertes (29.6 ± 4.4 años; 80.95 ± 9.57 kg; 12.2 ± 5.8% de grasa corporal; 2.4 ± 0.9 experiencia de entrenamiento) y débiles (28.5 ± 5.4 años; 79.4 ± 6.8 kg; 13.6 ± 3.5% de grasa corporal; 2.0 ± 1.1 experiencia de entrenamiento). Todos los voluntarios realizaron, en días diferentes, los test de una repetición máxima en los ejercicios: sentadilla por detrás, sentadilla por delante, arranque y cargada. Resultados: Los voluntarios clasificados con el mejor rendimiento en el arranque y en la cargada presentaron mayor fuerza en la sentadilla por detrás (154.9 ± 20.3 kg vs. 132.7 ± 11.6 kg; p = 0.009) y en la sentadilla por delante (139.0 ± 14.5 kg vs. 116.8 ± 11.3 kg; p = 0.002) cuando fueron comparados con los voluntarios más débiles. Además, se observaron correlaciones fuertes entre la fuerza relativa de la sentadilla por detrás (r = 0.83; r = 0.76; p = 0.001) y la sentadilla por delante (r = 0.73; r = 0.83; p = 0.001) con el rendimiento en los ejercicios de arranque y cargada, respectivamente. Conclusión: Por tanto, una mayor fuerza en ejercicios básicos, como la sentadilla por detrás y por delante, pueden contribuir para el rendimiento de los ejercicios olímpicos en practicantes de CrossFit®.

Polycarpol, a recurrent lanostane-type triterpene in Annonaceae family, was confirmed by thin layer chromatography and mass spectrometry analysis in the aerial parts (twigs and trunk barks) of Unonopsis duckei R.E. Fr., U. floribunda Diels, U. rufescens(Baill.) R.E. Fr., U. stipitata Diels, Onychopetalum amazonicum R.E. Fr. and Bocageopsis pleiospermaMaas. Its chemotaxonomic significance was discussed for these three genera, as well for the Annonaceae family. In addition, the antimicrobial activity against several strains of microorganisms was evaluated for the first time for this compound, being observed significant antibacterial activity against Staphylococcus aureus (ATCC 6538), Staphylococcus epidermidis (ATCC 1228) and Escherichia coli (ATCC 10538 and ATCC 10799) with minimal inhibitory concentration values between 25 and 50 μg ml−1.

O objetivo do presente estudo foi verificar a reprodutibilidade de variáveis específicas do teste anaeróbio de Wingate e de alguns marcadores fisiológicos e percetuais associados ao teste em ciclistas treinados. Quinze ciclistas do sexo masculino realizaram três testes, com intervalo mínimo de 48 horas entre cada sessão, com carga correspondente a 0.087 vezes a massa corporal de cada voluntário. Foram mensuradas a potência pico, potência média, potência mínima, índice de fadiga, frequência cardíaca, perceção subjetiva de esforço e concentração de lactato. Foi verificada a normalidade dos dados, aplicada Anova One Way para medidas repetidas, com post-hoc de Tukey, foi utilizado o coeficiente de correlação intraclasse e a técnica de concordância de Bland-Altman. Não foram encontradas diferenças significativas na potência pico, índice de fadiga, concentração de lactato, frequência cardíaca e perceção subjetiva de esforço entre os testes. Destas, a potência pico, frequência cardíaca e perceção subjetiva de esforço apresentaram valores elevados e significativos de coeficiente de correlação intraclasse entre cada teste e entre os três testes (variando entre .797 e .975). Ainda, a potência pico apresentou boa concordância entre os testes através da técnica de Bland-Altman. Em suma, o teste anaeróbio de Wingate apresentou alta reprodutibilidade para a potência pico, a frequência cardíaca e a perceção subjetiva de esforço em ciclistas treinados.

The aim of this study was to verify the reliability of selected variables during Wingate anaerobic test, physiologic markers and perceptual associated in trained cyclists. Fifteen male cyclists performed three tests, with workload of 0.087 times the body mass. Measures of peak power, average power, minimum power, fatigue index, heart rate, perceived effort and lactate concentration were collected. It was verified data normality, applied ANOVA One Way repeated with Tukey as post-hoc test, intraclass coefficient correlation and Bland Altman test. Results showed no significant different for the peak power, fatigue index, lactate concentration, heart rate and perceived effort between tests. Of these, peak power, heart rate and perceived effort high and significant intraclass correlation scores were found (.797−.975). Also, peak power showed good agreement between tests. In conclusion, the Wingate anaerobic test showed high reliability for peak power, heart rate and perceived effort in recreational cyclists.

The resistance of coffee plants to Meloidogyne exigua is conferred by the gene Mex-1. However, the mechanisms of resistance still need to be clarified. Therefore, the penetration, development and reproduction of two populations of M. exigua (M1, isolated from the coffee plant and M2, from rubber tree) in susceptible (Coffea arabica 'Catuai') and resistant (C. canephora 'Apoatã') cultivars were studied. A greater quantity of J2 from M1 penetrated the susceptible cultivar, but there was no difference between the cultivars for M2. Although the resistant coffee cultivar formed some galls, the nematode did not reproduce. M2 did not induce the formation of galls or the production of eggs in either cultivars. Events related to hypersensitive reaction (HR) were observed as well as other defense responses of the coffee cultivar against M. exigua, which inhibited the formation of the feeding site, provoked emigration of the J2 and delayed or inhibited development and reproduction. The response of the non-host plant was more effective, because it did not allow development of the nematode or, consequently, its reproduction. It was concluded that the coffee cultivar's resistance to M. exigua is not restricted to HR, but rather to a set of defense responses, both constitutive and induced, expressed after nematode penetration, especially phenolic-like compounds.

Activated carbons are universal adsorbents that can be obtained from a wide variety of raw materials, and have been found to be very effective for mercury removal from water. This contribution presents a comparative adsorption in column study of activated carbons obtained by physical activation of carbonized eucalyptus wood with water vapour, as adsorbents of mercury in liquid phase. Two samples were treated with sulphuric acid and with carbon disulphide, in order to introduce sulphur as a heteroatom onto the adsorbent surface. Adsorption in column assays was performed and the breakthrough curves were used to compare their performance. The sulphurization treatments produce important variations on the textural properties of the solid, on the zero charge point, and on the number of acid and basic surface groups. From the analysis of these factors, the dependency of the adsorption capacity of a solid with the pH of the solution can be explained.

The association of education, tobacco smoking, alcohol consumption, and interleukin-2 (IL-2 +114 and -384) and -6 (IL-6 -174) DNA polymorphisms with head and neck squamous cell carcinoma (HNSCC) was investigated in a cohort study of 445 subjects. IL-2 and IL-6 genotypes were determined by real-time PCR. Cox regression was used to estimate hazard ratios (HR) and 95% confidence intervals (95%CI) of disease-specific survival according to anatomical sites of the head and neck. Mean age was 56 years and most patients were males (87.6%). Subjects with 5 or more years of schooling had better survival in larynx cancer. Smoking had no effect on HNSCC survival, but alcohol consumption had a statistically significant effect on larynx cancer. IL-2 gene +114 G/T (HR = 0.52; 95%CI = 0.15-1.81) and T/T (HR = 0.22; 95%CI = 0.02-3.19) genotypes were associated with better survival in hypopharynx cancer. IL-2 +114 G/T was a predictor of poor survival in oral cavity/oropharynx cancer and larynx cancer (HR = 1.32; 95%CI = 0.61-2.85). IL-2 -384 G/T was associated with better survival in oral cavity/oropharynx cancer (HR = 0.80; 95%CI = 0.45-1.42) and hypopharynx cancer (HR = 0.68; 95%CI = 0.21-2.20), but an inverse relationship was observed for larynx cancer. IL-6 -174 G/C was associated with better survival in hypopharynx cancer (HR = 0.68; 95%CI = 0.26-1.78) and larynx cancer (HR = 0.93; 95%CI = 0.42-2.07), and C/C reduced mortality in larynx cancer. In general, our results are similar to previous reports on the value of education, smoking, alcohol consumption, and IL-2 and IL-6 genetic polymorphisms for the prognosis of HNSCC, but the risks due to these variables are small and estimates imprecise.

Pretreatment of Escherichia coli cultures with the iron chelator 2,2’-dipyridyl (1 mM) protects against the lethal effects of low concentrations of hydrogen peroxide (<15 mM). However, at H2O2 concentrations equal to or greater than 15 mM, dipyridyl pretreatment increases lethality and mutagenesis, which is attributed to the formation of different types of DNA lesions. We show here that pretreatment with dipyridyl (1 mM) prior to challenge with high H2O2 concentrations (≥15 mM) induced mainly G:C→A:T transitions (more than 100X with 15 mM and more than 250X with 20 mM over the spontaneous mutagenesis rate) in E. coli. In contrast, high H2O2 concentrations in the absence of dipyridyl preferentially induced A:T→T:A transversions (more than 1800X and more than 300X over spontaneous mutagenesis for 15 and 20 mM, respectively). We also show that in the fpg nth double mutant, the rpoB gene mutation (RifS-RifR) induced by 20 mM H2O2 alone (20X higher) was increased in 20 mM H2O2 and dipyridyl-treated cultures (110X higher), suggesting additional and/or different lesions in cells treated with H2O2 under iron deprivation. It is suggested that, upon iron deprivation, cytosine may be the main damaged base and the origin of the pre-mutagenic lesions induced by H2O2.

O cafeeiro é o hospedeiro tipo de Meloidogyne exigua, no qual a produção de inóculo em larga escala demanda tempo prolongado. Objetivou-se comparar a reprodução de populações de M. exigua em plantas de café e pimentão, e avaliar a possível ocorrência de seletividade fisiológica depois de sucessivas gerações em pimentão. A reprodução foi estudada em cinco populações e a seletividade envolveu uma população que foi mantida em plantas de café e pimentão por 30 meses, com as avaliações realizadas a cada 90 dias. O número de galhas e de ovos foi sempre superior em pimentão. A taxa reprodutiva de M. exigua foi cerca de quatro vezes maior em pimentão do que em cafeeiro. Não houve diferença na reprodução de M. exigua em cafeeiro, para as duas fontes de inóculo, cafeeiro e pimentão, durante os 30 meses. Conclui-se que o pimentão é melhor hospedeiro que o cafeeiro para a multiplicação de inóculo de M. exigua, inclusive, para as populações que não se multiplicam em tomateiro, com as vantagens da rapidez e facilidade de condução das plantas em casa de vegetação. Assim, a produção de grande quantidade de inóculo de M. exigua é possível pela utilização do pimentão, uma vez que o nematóide não perde sua habilidade de infectar o cafeeiro.

Coffee is the host-type of Meloidogyne exigua and significant inoculum production on this plant takes a long time. The objective of this study was to evaluate the reproduction of five M. exigua populations on coffee comparatively with reproduction on pepper, and the possible occurrence of physiological selectivity after successive generations on pepper. In the selectivity test, one population was maintained on coffee and pepper for 30 months and reproduction was evaluated 10 times, at 90 day intervals. The number of galls and eggs was always higher in pepper roots than in coffee ones. The reproductive rate in pepper was four times higher than in coffee. There was no difference in nematode reproduction in coffee between the two inoculum sources, coffee and pepper, during 30 months. Pepper proved to be a better host than coffee for the rearing of M. exigua, including those populations unable to reproduce in tomato roots, since pepper plants are easy to manage under green-house conditions and nematode reproduction is faster than in coffee. Therefore, pepper should be used to rear M. exigua since the nematode does not lose its ability to infect coffee.

O efeito dos extratos de sementes de nim e do extrato de sementes concentrado (NSC) foi avaliado na mortalidade e desenvolvimento de Heterodera glycines. A mortalidade de juvenis de segundo estádio (J2) foi avaliada na presença dos extratos de sementes (aquosos, metanólicos e hexânicos) e NCS in vitro. Em casa de vegetação, o número de fêmeas e de ovos por sistema radicular foi determinado 30 dias após a inoculação de plantas de soja com J2 tratados com os extratos de sementes. Os extratos hexânicos foram ineficientes em matar os J2, conquanto nenhuma diferença estatística (P>0.05) foi observada entre as doses estudadas e a testemunha. Os extratos aquosos e o metanólico a 1000 mg L-1 causaram a morte de pelo menos 98% dos J2, mas não houve diferença significativa entre estes tratamentos. A mortalidade dos J2 tratados com NSC cresceu exponencialmente com o aumento das doses (R²=0.94). O LD50 do NSC sobre J2 de H. glycines foi de 42.6 mg L-1. Em casa de vegetação, as maiores reduções no número de fêmeas, 84%, foram obtidas com o extrato aquoso (41.6 mg L-1) e metanólico (1000 mg L-1). Da mesma forma, as maiores reduções no número de ovos, cerca de 90%, foram obtidas com os mesmos extratos. Considerou-se que o uso do extrato aquoso em condições de campo é mais apropriado que os demais extratos. As atividades do extrato metanólico e do NSC foram atribuídas a sete tetranortriterpenóides (azadiractina H, azadiractina A, azadiractina B, desacetilnimbim, desacetilsalanim, nimbim and salanim) que foram identificados por cromatografia líquida de alta performance de fase reversa.

The effect produced by neem seed extracts and a neem seed concentrate (NSC) on the mortality and development of Heterodera glycines was evaluated. Second-stage juvenile (J2) mortality produced by the extracts and NSC was determined. Soybean seedlings treated with each of the extracts (aqueous, methanolic and hexane) were inoculated with J2 and the number of females and eggs per root system determined after 30 days. Both the hexane extracts were ineffective as no statistical difference (P>0.05) in J2 mortality was observed between them and the control. No statistical difference (P>0.05) in J2 mortality (>98%) was obtained between the water extracts and methanol extract at 1000 mg L-1 treatments. The J2 percentage of mortality exponentially increased with the NSC dosages (R²=0.94). LD50 was 42.6 mg L-1 for NSC on H. glycines. In the greenhouse experiment, the highest reduction in the number of females was 84%, obtained with the aqueous (41.6 mg L-1) and methanolic extracts (1000 mg L-1). In the same way, the highest reduction in the number of eggs, of about 90%, was obtained with the same extracts. Thus, using aqueous extracts is more suitable than the remaining extracts in field conditions. The activities of the methanol and NSC extracts were attributed to the seven tetranortritepenoids (azadirachtin H, azadirachtin A, azadirachtin B, desacetylnimbin, desacetylsalannin, nimbin and salannin) identified by reverse phase high performance liquid chromatography.

The genital localized myiasis is a rare zooparasitic dermatosis. This case reported to a penile myiasis by Dermatobia hominis larvae in a 62 year-old man, who is a retired military official from São Gonçalo city, Rio de Janeiro state, Brazil. This patient was guided to the sexually transmitted diseases (STD) clinic from Universidade Federal Fluminense (UFF) by professionals from a urology outpatient clinic of a public hospital from Rio de Janeiro state. The exam showed a 2 cm diameter nodular lesion with a hyperemic area in the glands penis that had arisen 20 days before the exam. It evolved in a favorable way after larvae removal, through previous local anesthesia and slight pressure in lesion's base with the extraction of the larvae. Penile myiasis is not a very frequent situation, although it must be considered as an option in differential diagnosis of genital infectious diseases, even among people of low economical level, as in the case presented.

Empregou-se a estimulação elétrica neuromuscular (EENM) de baixa freqüência no músculo quadríceps femoral de cães com atrofia induzida e avaliou-se a ocorrência de ganho de massa nessa musculatura. Foram utilizados oito cães com pesos entre 15 e 30kg, distribuídos aleatoriamente em dois grupos denominados de I ou controle e II ou tratado. A articulação femorotibiopatelar esquerda foi imobilizada por 30 dias pelo método de transfixação percutânea tipo II, com retirada de aparelho de imobilização após esse período. Decorridas 48 horas da remoção, foi realizada a EENM nos cães do grupo II, cinco vezes por semana, com intervalo de 24 horas cada sessão, pelo período de 60 dias. Foram avaliadas a circunferência da coxa, a goniometria do joelho, a análise clínica da marcha, as enzimas creatina-quinase (CK) e aspartato-amino-transferase (AST) e a morfometria das fibras musculares em cortes transversais do músculo vasto lateral colhido mediante biópsia muscular. A EENM foi empregada no músculo quadríceps femoral na freqüência de 50Hz, duração de pulso de 300 milisegundos e relação de tempo on/off de 1:2. Quanto à morfometria das fibras do músculo vasto lateral, no grupo tratado houve aumento significativo (P<0,05) da área transversal aos 90 dias em relação ao dia zero. A EENM de baixa freqüência ocasiona hipertrofia do músculo vasto lateral em cães após a imobilização rígida temporária da articulação do joelho.

Low frequency neuromuscular electrical stimulation (NMES) was used on the femoral quadriceps of dogs with induced muscular atrophy and the occurrence of gain in mass in these muscles was evaluated. Eight dogs from 15 to 30kg were randomly distributed in two groups named I, or control; and II, or treated. For the induction of muscular atrophy, the left femoral-tibial-patellar joint was immobilized for 30 days by percutaneous transfixation type II. After 30 days, the immobilization device was removed. The NMES treatment began 48 hours after the removal of the immobilization device of the dogs of group II, and it was carried out five times per week with an interval of 24 hours between each session, for 60 days. The following parameters were measured: thigh circumference, goniometry of the knee, clinical gait analysis, creatine kinase (CK) and aspartate aminotransferase (AST) enzymes, and morphometry of the muscular fibers in transversal cuts of the vastus lateralis muscle collected through muscular biopsy. The NMES was applied on the femoral quadriceps at a frequency of 50 Hz, with pulse duration of 300 milliseconds, and the on/off time was at a proportion of 1:2. Regarding the morphometry of the vastus lateralis fibers, a significant increase (P<0.05) in the transversal area of the treated group at 90 days was observed when compared with that identified at the time of immobilization. Thus, it can be concluded that low frequency NMES brings about hypertrophy of the vastus lateralis muscle in dogs after temporary rigid immobilization of the knee joint.

MicroRNAs (miRNAs) are a class of small endogenous RNAs that play important regulatory roles by targeting mRNAs for cleavage or translational repression. miRNAs act in diverse biological processes including development, cell growth, apoptosis, and hematopoiesis, suggesting their association with cancer. We determined the miRNA expression profile of chronic and acute lymphocytic leukemias (CLL and ALL) using the TaqMan® MicroRNA Assays Human Panel (Applied Biosystems). Pooled leukemia samples were compared to pooled CD19+ samples from healthy individuals (calibrator) by the 2-DDCt method. Total RNA input was normalized based on the Ct values obtained for hsa-miR-30b. The five most highly expressed miRNAs were miR-128b, miR-204, miR-218, miR-331, and miR-181b-1 in ALL, and miR-331, miR-29a, miR-195, miR-34a, and miR-29c in CLL. To our knowledge, this is the first report associating miR-128b, miR-204 and miR-331 to hematological malignancies. The miR-17-92 cluster was also found to be up-regulated in ALL, as previously reported for some types of lymphomas. The differences observed in gene expression levels were validated for miR-331 and miR-128b in ALL and CD19+ samples. These miRNAs were up-regulated in ALL, in agreement with our initial results. A brief target analysis was performed for miR-331. One of its putative targets, SOCS1, promotes STAT activation, which is a known mediator of cell proliferation and survival, suggesting the possibility of an association between miR-331 and these processes. This initial screening provided information on miRNA differentially expressed in normal and malignant B-cells that could suggest the potential roles of these miRNAs in hematopoiesis and leukemogenesis.