Abstract Textile sludge is a waste from the effluent treatment of textile laundries and its incorporation into alternative materials has been proposed as a possibility for its disposal. A systematic search was carried out in literature from this perspective, aiming to observe concentrations of waste material and its preparation, matrices used, technological performance and immobilization of textile waste. The matrices used were mortars, concretes and ceramic bricks, in general, deleterious effects were observed on mechanical characteristics of the materials produced. In concrete and mortars, the fineness had an impact on increase in water demand, which increased mixtures porosity; and organic matter and heavy metals, in cement hydration. Recent studies therefore proposed waste material pre-treatment as a favorable alternative. Increases in porosity also occurred in ceramic bricks, however, due to high organic matter content. Regarding waste immobilization, cementitious materials showed satisfactory performance; ceramic bricks, on the other hand, obtained some results outside the normative limits. Therefore, it is necessary to expand discussions regarding the pre-treatment carried out on textile sludge, as well as its impacts on mechanical and environmental performance. disposal perspective preparation mortars bricks general produced demand metals hydration pretreatment pre however content hand limits Therefore

Resumo Olodo têxtil é um resíduo do tratamento do efluente das lavanderias têxteis e sua incorporação em materiais alternativos tem sido proposta como possibilidade para sua destinação. Foi realizada uma busca sistemática na literatura nesta perspectiva, visando observar as concentrações de material residual e seu preparo, as matrizes utilizadas, o desempenho tecnológico e imobilização do resíduo têxtil. As matrizes empregadas foram argamassas, concretos e tijolos cerâmicos, sendo observados, de maneira geral, efeitos deletérios nas características mecânicas dos materiais produzidos. Em concretos e argamassas, a finura impactou no aumento da demanda de água, o que elevou a porosidade das misturas; e a matéria orgânica e metais pesados, na hidratação do cimento. Estudos recentes propuseram, então, o pré-tratamento do material residual como uma alternativa favorável. Incrementos na porosidade também ocorreram nos tijolos cerâmicos, porém, devido ao elevado teor de matéria orgânica. Quanto a imobilização do resíduo, os materiais cimentícios apresentaram desempenho satisfatório; já os tijolos cerâmicos, obtiveram alguns resultados fora dos limites normativos. Portanto, é necessário ampliar as discussões quanto ao pré-tratamento realizado no lodo têxtil, bem como seus impactos no desempenho mecânico e ambiental. destinação perspectiva preparo utilizadas argamassas cerâmicos observados geral produzidos água misturas pesados cimento propuseram então prétratamento pré favorável porém satisfatório normativos Portanto ambiental

Abstract: Introduction: Health care by nursing professionals to people with gender diversity can be negatively affected by the heteronormativity that has historically governed society. It is challenging to safeguard and seek actions to provide inclusive humanized care Objective: To understand the nursing professional’s experiences when providing humanized care to people with gender diversity. Method: Qualitative research with a phenomenological approach. Four nursing professionals were interviewed. The information was collected through semi-structured individual and group interviews; the discourses were analyzed, and central concepts were triangulated.Results: Humanized care is manifested through three dimensions: experiences in providing humanized care, factors that facilitate the delivery of humanized care and factors that hinder it. The categories included in the first dimension are equality, empathy, respect and learning. Factors that facilitate humanized care have the categories of welcoming and continuing education, and factors that hinder it have the categories of social name and suspicion. Conclusion: Humanized care for people with gender diversity requires values of equality and empathy, together with inclusive training from undergraduate level. Deficiency in the development of soft skills hinders the nurse-patient relationship, while suspicion of the gender-diverse person hinders first consultations. The integration of the social name and continuous training are essential to promote an environment of trust and reduce the feeling of vulnerability and discrimination.

Resumo: Introdução: O atendimento em saúde prestado pelos profissionais de enfermagem às pessoas com diversidade de gênero pode ser afetado negativamente pela heteronormatividade que historicamente rege a sociedade. Atualmente, é um desafio resguardar e buscar ações para proporcionar um cuidado humanizado e inclusivo. Objetivo: Compreender as vivências do profissional de enfermagem ao prestar o cuidado humanizado a pessoas com diversidade de gênero. Método: Pesquisa qualitativa, com abordagem fenomenológica. Foram entrevistados quatro profissionais de enfermagem. As informações foram coletadas por meio de entrevistas semiestruturadas individuais e em grupo; os discursos foram analisados e foi realizada uma triangulação dos principais conceitos. Resultados: O cuidado humanizado se manifesta por meio de três dimensões: vivências ao fornecer cuidados humanizados, fatores que facilitam a prestação de cuidado humanizado e fatores que a dificultam. As categorias incluídas na primeira dimensão são igualdade, empatia, respeito e aprendizado. Os fatores que facilitam os cuidados humanizados incluem acolhimento e formação contínua, enquanto os fatores que os dificultam incluem o uso do nome social e o receio. Conclusão: O cuidado humanizado a pessoas com diversidade de gênero requer valores de igualdade e empatia, juntamente com formação inclusiva desde o nível de graduação. A deficiência no desenvolvimento de habilidades interpessoais dificulta o relacionamento enfermeiro-paciente, enquanto o receio da pessoa com diversidade de gênero dificulta as primeiras consultas. A integração do nome social e a formação continuada são essenciais para promover um ambiente de confiança e reduzir a sensação de vulnerabilidade e discriminação.

Resumen: Introducción: La atención en salud de los profesionales de enfermería a personas con diversidad de género puede verse afectada negativamente por la heteronormatividad que ha regido históricamente la sociedad. Actualmente es un desafío resguardar y buscar acciones para entregar un cuidado humanizado inclusivo. Objetivo: Comprender las vivencias del profesional de enfermería al brindar el cuidado humanizado a personas con diversidad de género. Método: Investigación cualitativa, con un enfoque fenomenológico. Se entrevistó a cuatro profesionales de enfermería. La información se recopiló a través de entrevistas individuales y grupales semiestructuradas; los discursos fueron analizados y se realizó una triangulación de los conceptos principales. Resultados: El cuidado humanizado se manifiesta a través de tres dimensiones: vivencias al brindar cuidados humanizados, factores que facilitan la entrega del cuidado humanizado y factores que lo dificultan. Las categorías incluidas en la primera dimensión son igualdad, empatía, respeto y aprendizaje. Los factores que facilitan los cuidados humanizados tienen como categorías la acogida y la formación continua, y los factores que lo dificultan tienen las categorías de nombre social y recelo. Conclusión: El cuidado humanizado a personas con diversidad de género requiere valores de igualdad y empatía, junto con una formación inclusiva desde pregrado. La deficiencia en el desarrollo de habilidades blandas dificulta la relación enfermero-paciente, mientras que el recelo de la persona con diversidad de género obstaculiza las primeras consultas. La integración del nombre social y la formación continua son esenciales para promover un ambiente de confianza y reducir la sensación de vulnerabilidad y discriminación.

ABSTRACT The recent global health crisis highlighted the fundamental role of family farming and public procurement policies in guaranteeing the human right to adequate food. This documentary analysis compares public procurement experiences (2019 to 2022) in a region of Brazil and another in Colombia, focusing on family farming from a gender perspective to identify challenges and best practices. Among the findings, it is noteworthy that although structured policies for public procurement already exist in Brazil, regional realities pointed to challenges during the period. Advances in Colombia were noted with the issuance of Law 2046 of 2020. In both countries, the low role of women in the ownership of rural properties and decision-making was evident, despite their participation in the rural family productive workforce.

RESUMEN La pasada crisis sanitaria mundial evidenció el papel fundamental de la agricultura familiar y las políticas de compras pública para garantizar el derecho humano a la alimentación adecuada. En este análisis documental, se compararon las experiencias de compras públicas (2019 a 2022), en una región de Brasil y otra de Colombia, de la agricultura familiar, desde una perspectiva de género, con el objetivo de identificar desafíos y buenas prácticas. Entre los hallazgos, se destaca que, aunque ya existen políticas estructuradas para las compras públicas en Brasil, la realidad regional señaló desafíos en el período. Se señalaron los avances en Colombia, con la expedición de la Ley 2046 de 2020. En ambos países se evidenció el bajo rol de la mujer en la titulación de las propiedades rurales y en la toma de decisiones, aunque se evidencia su participación en la fuerza productiva rural familiar.

Abstract This article discusses questions concerning the future of humanity in the face of threats to the health of populations, whose impact has been exacerbated in the course of inequalities in all parts of the world, pari passu with global development in the hegemonized model since last century. The COVID-19 pandemic is a good example that illustrates this dissonance between development and inequalities. Questions were formulated to be debated about the construction of the future of world society, based on the understanding of the evolutionary character of life on the planet vis-à-vis the evils that affect large contingents of the population and represent powerful risks for this evolutionary process. These questions call attention to the discussion around social participation in the definition and control of public policies, as opposed to the hegemony of private interests in the formulation and execution of these policies, both in the scenarios of each country and in the international context. populations century COVID19 COVID 19 COVID-1 society visàvis vis à process policies context COVID1 1 COVID-

Resumo O artigo discute questões sobre o futuro da humanidade ante as ameaças que rondam a saúde das populações, cujo impacto vem se exacerbando no curso das desigualdades em todas as partes do mundo, pari passu o desenvolvimento global no modelo hegemonizado a partir do século passado. A pandemia de COVID-19 foi tomada como um caso que bem ilustra essa dessintonia entre desenvolvimento e desigualdades. Formulam-se perguntas a serem postas em debate sobre a construção do futuro da sociedade mundial, com base na acepção sobre o caráter evolucional da vida no planeta vis-à-vis os males que acometem grandes contingentes populacionais e representam poderosos riscos para esse processo evolutivo. São indagações que apontam para a discussão em torno da participação social na definição e no controle das políticas públicas, em contrapartida à hegemonia dos interesses privados na formulação e execução dessas políticas, tanto nos cenários de cada país como no contexto internacional. populações mundo passado COVID19 COVID 19 COVID-1 Formulamse Formulam mundial visàvis vis evolutivo públicas internacional COVID1 1 COVID-

Resumo O artigo discute questões sobre o futuro da humanidade ante as ameaças que rondam a saúde das populações, cujo impacto vem se exacerbando no curso das desigualdades em todas as partes do mundo, pari passu o desenvolvimento global no modelo hegemonizado a partir do século passado. A pandemia de COVID-19 foi tomada como um caso que bem ilustra essa dessintonia entre desenvolvimento e desigualdades. Formulam-se perguntas a serem postas em debate sobre a construção do futuro da sociedade mundial, com base na acepção sobre o caráter evolucional da vida no planeta vis-à-vis os males que acometem grandes contingentes populacionais e representam poderosos riscos para esse processo evolutivo. São indagações que apontam para a discussão em torno da participação social na definição e no controle das políticas públicas, em contrapartida à hegemonia dos interesses privados na formulação e execução dessas políticas, tanto nos cenários de cada país como no contexto internacional.

Abstract This article discusses questions concerning the future of humanity in the face of threats to the health of populations, whose impact has been exacerbated in the course of inequalities in all parts of the world, pari passu with global development in the hegemonized model since last century. The COVID-19 pandemic is a good example that illustrates this dissonance between development and inequalities. Questions were formulated to be debated about the construction of the future of world society, based on the understanding of the evolutionary character of life on the planet vis-à-vis the evils that affect large contingents of the population and represent powerful risks for this evolutionary process. These questions call attention to the discussion around social participation in the definition and control of public policies, as opposed to the hegemony of private interests in the formulation and execution of these policies, both in the scenarios of each country and in the international context.

Abstract Cotton is an important fiber crop, with special significance in the historical, cultural, and socio- economic development of the planet. In Peru, the great color variability of cotton fibers has allowed the commercialization of numerous textile products throughout the centuries. At present, cotton species native to South America, such as Gossypium barbadense, are constantly threatened by anthropogenic factors, and thus there is a clear need for species conservation. Therefore, this study aimed to collect G. barbadense germplasm in Peru, characterize the germplasm by fiber color, and evaluate the application of different methods of germplasm conservation. The evaluated methods were seed bank, field techniques, and in vitro culture. Two hundred seventy-six samples of G. barbadense were collected between 2017 and 2021 and classified by fiber color into monochrome and polychrome of white (114 samples), orange (107) and brown (41). The conservation methods showed that the seed bank samples ranged from 50 g to 1 kg, while 95% of the field accessions survived. In addition, seedlings presented positive developmental responses when they were grown in vitro under modified MS culture, sucrose 2.0%, vitamins, glycine 2.0 mgL-1, and AgNO3 2.0 mg L-1. This study provides evidence of the color variability of G. barbadense distributed throughout Peru, and confirms that this species can be managed and conserved using germplasm conservation methods.

Abstract Potato plants are often subjected to heat stress during growth and development, resulting in loss of yield and quality. However, the possibility exists that commercially important genotypes show tolerance to heat stress. In this study, we aim to investigate the morphological and physiological effects of moderate conditions of heat stress on tuberization in nine potato genotypes, and how it affects propagation using an ex vitro and in vitro system. The plant material came from the In Vitro Germplasm Bank of the International Potato Center (CIP). The incubation temperatures of the cultures in in vitro and ex vitro conditions were the ambient in the laboratory and greenhouse, respectively. In in vitro system a randomized complete block design was employed in nine genotypes, two treatments: multiplication culture medium and the same culture medium supplemented with 8.0% sucrose and 500 mg/L-1 CCC, six flasks and five explants per experimental unit. Among all genotypes evaluated, Leona and Liberteña did not form tubers under ex vitro treatment conditions. Under in vitro conditions, Amarilis, Capiro, Perricholi and Tacna showed no evidence of tuberization, and Canchán was the only genotype where 100% formation of tuberous structures were produced in all evaluations. The findings concluded in this study showed that of all the potatoes evaluated, Canchán best responded to the evaluations under both in ex vitro and in vitro conditions, at maximum temperatures between 22.4 to 26.9 and 27.0 to 28.2 oC, respectively. Therefore, it is vitally important to consider its development potential in different producing locations around the world, which are directly and indirectly affected by climate change.

SUMMARY OBJECTIVE: The aim of this study was to evaluate the prevalence of early neonatal sepsis in pregnant women with a positive culture for group B beta-hemolytic Streptococcus in a middle-income city in Southeastern Brazil. METHODS: A retrospective cohort study was conducted, involving singleton low- and high-risk pregnancies in whom group B beta-hemolytic Streptococcus cultures were evaluated between 35 and 37 weeks of gestation using vaginal and anal swabs. A specific medium (Todd-Hewitt) was used for culturing. The pregnant women were divided into two groups based on positive (n==201) and negative (n==420) cultures for group B beta-hemolytic Streptococcus. RESULTS: The maternal colonization rate by group B beta-hemolytic Streptococcus was 32.3%. The prevalence of early neonatal sepsis was 1.0% (2/201) among patients with a positive group B beta-hemolytic Streptococcus culture and 1.9% (8/420) among patients with a negative culture. Among the patients who underwent adequate prophylaxis, crystalline penicillin G was used in 51.9% (54/104), followed by cefazolin in 43.3% (45/104), ampicillin in 3.8% (4/104), and clindamycin in 1.0% (1/104). A model that included prematurity (p==0.001) proved to be an independent risk predictor of early neonatal sepsis [χ2 (1)==15.0, odds ratio: 16.9, 95% confidence interval: 4.7–61.6, p<0.001, Nagelkerke R2==0.157]. CONCLUSION: The prevalence of a positive culture for group B beta-hemolytic Streptococcus was high. However, the prevalence of early neonatal sepsis was low in pregnant women with both positive and negative group B beta-hemolytic Streptococcus cultures and in pregnant women with a positive culture who underwent both adequate and inadequate antibiotic prophylaxis. Prematurity proved to be an independent predictor of early neonatal sepsis, considering the entire study population.

El objetivo de este estudio fue comprender las motivaciones y las implicaciones del cuidado de familiares ancianos dependientes realizado por hombres. A partir de una investigación cualitativa realizada entre julio y diciembre de 2018 en seis ciudades en las cinco regiones brasileñas, se entrevistaron 11 hombres que desempeñan la tarea de cuidadores informales. Los hombres asumieron esta tarea porque las mujeres estaban exhaustas y enfermas y por reciprocidad afectiva y marital. Los hijos manifestaron incomodidad en la administración de cuidados personales a los padres, mientras que los cónyuges presentaron dificultades con actividades domésticas. Los cuidadores solitarios estaban cansados, con dolores, privación de sueño, síntomas depresivos y soledad. El soporte prestado por familiares y cuidadores formales se mostró valioso para disminuir el sufrimiento de los cuidadores que titubeaban en pedir ayuda. Existe la necesidad de que los profesionales de salud estén atentos a los prestadores de cuidados informales a los familiares. 201 brasileñas 1 marital padres domésticas cansados dolores sueño soledad ayuda 20 2

The objective of study was to understand the motivations and implications of care given by men for dependent elderly family members. Qualitative research happened between July and December 2018 in six cities in the five Brazilian regions, interviewing 11 men who perform tasks as informal caregivers. The men assumed this task because the women’s exhaustion and sickness, added to affective and marital reciprocity. Their sons expressed discomfort in taking personal care of their parents, while the spouses had difficulties with domestic activities. Lonely caregivers were tired, in pain, sleep deprived, experiencing depressive symptoms and loneliness. Support provided by family members and formal caregivers proved to be valuable in alleviating the suffering of caregivers who were reluctant to ask for help. Health professionals are called to pay attention to informal care providers for family members. 201 regions 1 womens women s sickness reciprocity parents activities tired pain deprived loneliness help 20 2

O objetivo deste estudo foi compreender as motivações e as implicações do cuidado de familiares idosos dependentes realizado por homens. Com base em pesquisa qualitativa realizada entre julho e dezembro de 2018 em seis cidades nas cinco regiões brasileiras, foram entrevistados 11 homens que desempenham a tarefa de cuidadores informais. Eles assumiram essa tarefa em razão de as mulheres estarem exaustas e adoecidas, assim como por reciprocidade afetiva e marital. Os filhos manifestaram desconforto na administração de cuidados pessoais aos pais, enquanto os cônjuges apresentaram dificuldades com atividades domésticas. Os cuidadores solitários estavam cansados, com dores, privação do sono, sintomas depressivos e solidão. O suporte prestado por familiares e cuidadores formais mostrou-se valioso para amenizar o sofrimento dos cuidadores que relutavam em pedir ajuda. Há necessidade de os profissionais de saúde estarem atentos aos prestadores de cuidados informais aos familiares. 201 brasileiras 1 adoecidas marital pais domésticas cansados dores sono solidão mostrouse mostrou se ajuda 20 2

ABSTRACT We present a rare case of primary caruncle basal cell carcinoma (BCC), a condition with limited occurrences. Our patient, an 80-year-old woman without prior ocular pathological history, presented a 2x2mm pedunculated blackish nodular lesion on the caruncle of her left eye, without local conjunctival or cutaneous involvement. Histological analysis following complete excision confirmed the presence of basal cell carcinoma within the caruncle. Over a span of 30 months, no recurrence has been observed. While scant cases are documented in the literature, we conducted a review of these instances. Despite its infrequent manifestation, this condition should be taken into account when evaluating caruncular tumors, given its tendency to invade the orbit. Complete excision with free surgical margins is the treatment of choice, and adjuvant radiotherapy or chemotherapy might be considered. BCC, BCC , (BCC) occurrences patient 80yearold yearold 80 year old history xmm x mm eye involvement 3 months observed literature instances manifestation tumors orbit choice considered (BCC 8

ABSTRACT This paper explores the relationship between adherent utterances in architectural objects and the formation of discursive ethos in São Paulo’s residential architecture. To that end, it uses the theoretical and methodological principles of discourse analysis and research in toponymy and focuses on a central area of the city. Such area was selected because it is experiencing an ever-growing real state transformation process, in which several construction stages can be observed. The temporal-thematic focus of the study was defined by the affirmation of modern architecture, up to the launch of contemporary new properties. The study is carried out by searching for adherent utterances in urban landscape, looking at the adherence relationship between the utterance and its support, establishing suitable observation parameters for the adherence utterances identified, and analyzing and characterizing the utterances in accordance with the architectural language of their supports. The results based on the considerations on scenography and interdiscursive relations suggest that the adherent statements constitute a privileged place for observing architecture and urban changes, as well as the discourses on them, in the central area of the city of São Paulo. Paulos Paulo s end evergrowing ever growing process observed temporalthematic temporal thematic properties landscape support identified supports changes them

RESUMO Este trabalho investiga a relação entre enunciação aderente e objetos arquitetônicos, discutindo os efeitos de sentido depreendidos pelos dispositivos discursivos de cenografia e ethos na arquitetura residencial paulistana. Com base nos princípios teóricos e metodológicos da análise do discurso e pesquisas na área da toponímia, é estruturada uma pesquisa de recorte geográfico com foco numa área central da cidade de São Paulo sujeita a diversas etapas de construção e em processo de transformação imobiliária crescente, com recorte temático-temporal definido pela afirmação da arquitetura moderna ao lançamento de novos imóveis na atualidade. Após considerações sobre os enunciados aderentes na paisagem urbana e a relação de aderência entre o enunciado e seu suporte, passa-se ao detalhamento do corpus delimitado para análise. Estabelecem-se, em seguida, os parâmetros de observação para o estudo dos enunciados aderentes, analisando-os e caracterizando-os de acordo com a área e a linguagem arquitetônica delimitada. Finalmente, as considerações sobre cenografia e relações interdiscursivas permitem concluir que os enunciados aderentes constituem um lugar privilegiado de observação da arquitetura, das transformações urbanas e dos discursos sobre tais transformações, na área central da cidade de São Paulo. arquitetônicos paulistana toponímia crescente temáticotemporal temático temporal atualidade suporte passase passa se Estabelecemse, Estabelecemse Estabelecem se, Estabelecem-se seguida analisandoos analisando caracterizandoos caracterizando delimitada Finalmente

FLAVONOIDS AND PHENOLIC ACIDS ISOLATED FROM Macrolobium acaciifolium (FABACEAE) LEAVES AND BRANCHES METHANOLIC EXTRACTS. The phytochemical study of Macrolobium acaciifolium extracts allowed the isolation and identification of 18 substances, 15 of which are reported for the first time in this species. From the methanolic extract of the leaves, the flavonoids luteolin-3’-O-α-L-rhamnoside (1), neoastilbin (2), astilbin (3), neoisoastilbin (4), isoastilbin (5), quercetin-3-O-α-L-rhamnoside (6), quercetin-3-O-rutinoside (7), kaempferol-3-O-rutinoside (8), quercetin-3-O-glicoside (9) and kaempferol-7-O-rutinoside (10) were isolated. From the methanolic extract of branches, the flavonoids chrysoeriol (13), apigenin (14), 7,4’-dihydroxyflavone (16), eriodictyol (17) and luteolin (18), in addition to the phenolic acids 3,4-dihydroxybenzoic acid (11), 3-methoxy,4-hydroxybenzoate (12) and methyl 3,4-dihydroxybenzoate (15) were isolated. The structural identification of the compounds was established by proton nuclear magnetic resonance (1H NMR), carbon-13 nuclear magnetic resonance (13C NMR), heteronuclear single quantum coherence (HSQC), heteronuclear multiple bond correlation (HMBC) and correlated spectroscopy (COSY) analyses. In this paper, we also discuss about the anisotropic effects on H-1”, H-2”, H-5” and H-6” rhamnose of isomers dihydroflavonoids. FABACEAE (FABACEAE EXTRACTS 1 substances species leaves luteolin3OαLrhamnoside luteolinOαLrhamnoside 3 O α L rhamnoside 1, , (1) 2, 2 (2) 3, (3) 4, 4 (4) 5, 5 (5) quercetin3OαLrhamnoside quercetinOαLrhamnoside quercetin 6, 6 (6) quercetin3Orutinoside quercetinOrutinoside rutinoside 7, 7 (7) kaempferol3Orutinoside kaempferolOrutinoside kaempferol 8, 8 (8) quercetin3Oglicoside quercetinOglicoside glicoside 9 (9 kaempferol7Orutinoside 10 (10 isolated branches 13, 13 (13) 14, 14 (14) 7,4dihydroxyflavone 74dihydroxyflavone dihydroxyflavone 7,4 16, 16 (16) 17 (17 18, (18) 3,4dihydroxybenzoic 34dihydroxybenzoic dihydroxybenzoic 3,4 11, 11 (11) 3methoxy,4hydroxybenzoate 3methoxy4hydroxybenzoate methoxyhydroxybenzoate methoxy,4 hydroxybenzoate methoxy 12 (12 3,4dihydroxybenzoate 34dihydroxybenzoate dihydroxybenzoate (15 1H H NMR, NMR NMR) carbon13 carbon carbon-1 13C C HSQC, HSQC (HSQC) HMBC (HMBC COSY (COSY analyses paper H1, H1 H-1” H2, H2 H-2” H5 H-5 H6 H-6 dihydroflavonoids OαLrhamnoside (1 (2 (3 (4 (5 (6 Orutinoside (7 (8 Oglicoside ( (13 (14 4dihydroxyflavone 74 (16 (18 4dihydroxybenzoic 34 (11 3methoxy 4hydroxybenzoate methoxy4 methoxy, 4dihydroxybenzoate carbon1 carbon- (HSQC H-1 H-2 H-

ABSTRACT. Citrus tristeza virus (CTV) can be transmitted by several aphid species in a semi-persistent mode, with Toxoptera citricida being the most efficient vector. In Brazil, mild CTV isolates are used for pre-immunization of citrus trees against severe isolates. We aimed to determine the capacity and efficiency of T. citricida in separating the viral complex into haplotypes from three well-characterized CTV isolates (PIAC, CS1, and Forte Rolândia). Single-aphid transmission assays were conducted to determine CTV transmission efficiency. The results showed that T. citricida transmitted only haplotypes from the PIAC and CS1 isolates, with efficiencies of 8 and 4%, respectively. Both isolates caused mild CTV symptoms in Brazil. However, isolate Forte Rolândia, which causes severe symptoms in citrus trees, was not transmitted by T. citricida. The detection of CTV haplotypes from PIAC and CS1 isolates in sweet orange (Citrus sinensis) plants after a single aphid transmission occurred at different time points. The first haplotype observed was from PIAC 150 days after citrus was challenged by a single aphid, followed by CS1 at day 210 after transmission. In addition, differences in the single-strand conformation polymorphism patterns between the CTV isolates and CTV haplotypes were determined, suggesting that an aphid can acquire and transmit only one CTV haplotype to citrus plants during phloem feeding. The study of the mechanism of transmission by the vector can increase our knowledge of the interactions among hosts, vectors, and pathogens, which are often neglected. ABSTRACT (CTV semipersistent semi persistent mode Brazil preimmunization pre immunization T wellcharacterized well characterized PIAC, (PIAC CS Rolândia. Rolândia . Rolândia) Singleaphid Single 4 4% respectively However sinensis points 15 21 addition singlestrand strand determined feeding hosts vectors pathogens neglected 1 2