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Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil Brasil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
; Slipinski, Adam
; Linzmeier, Adelita M.
; Calor, Adolfo R.
; Garda, Adrian A.
; Kury, Adriano B.
; Fernandes, Agatha C.S.
; Agudo-Padrón, Aisur I.
; Akama, Alberto
; Silva Neto, Alberto M. da
; Burbano, Alejandro L.
; Menezes, Aleksandra
; Pereira-Colavite, Alessandre
; Anichtchenko, Alexander
; Lees, Alexander C.
; Bezerra, Alexandra M.R.
; Domahovski, Alexandre C.
; Pimenta, Alexandre D.
; Aleixo, Alexandre L.P.
; Marceniuk, Alexandre P.
; Paula, Alexandre S. de
; Somavilla, Alexandre
; Specht, Alexandre
; Camargo, Alexssandro
; Newton, Alfred F.
; Silva, Aline A.S. da
; Santos, Aline B. dos
; Tassi, Aline D.
; Aragão, Allan C.
; Santos, Allan P.M.
; Migotto, Alvaro E.
; Mendes, Amanda C.
; Cunha, Amanda
; Chagas Júnior, Amazonas
; Sousa, Ana A.T. de
; Pavan, Ana C.
; Almeida, Ana C.S.
; Peronti, Ana L.B.G.
; Henriques-Oliveira, Ana L.
; Prudente, Ana L.
; Tourinho, Ana L.
; Pes, Ana M.O.
; Carmignotto, Ana P.
; Wengrat, Ana P.G. da Silva
; Dornellas, Ana P.S.
; Molin, Anamaria Dal
; Puker, Anderson
; Morandini, André C.
; Ferreira, André da S.
; Martins, André L.
; Esteves, André M.
; Fernandes, André S.
; Roza, André S.
; Köhler, Andreas
; Paladini, Andressa
; Andrade, Andrey J. de
; Pinto, Ângelo P.
; Salles, Anna C. de A.
; Gondim, Anne I.
; Amaral, Antonia C.Z.
; Rondón, Antonio A.A.
; Brescovit, Antonio
; Lofego, Antônio C.
; Marques, Antonio C.
; Macedo, Antonio
; Andriolo, Artur
; Henriques, Augusto L.
; Ferreira Júnior, Augusto L.
; Lima, Aurino F. de
; Barros, Ávyla R. de A.
; Brito, Ayrton do R.
; Romera, Bárbara L.V.
; Vasconcelos, Beatriz M.C. de
; Frable, Benjamin W.
; Santos, Bernardo F.
; Ferraz, Bernardo R.
; Rosa, Brunno B.
; Sampaio, Brunno H.L.
; Bellini, Bruno C.
; Clarkson, Bruno
; Oliveira, Bruno G. de
; Corrêa, Caio C.D.
; Martins, Caleb C.
; Castro-Guedes, Camila F. de
; Souto, Camilla
; Bicho, Carla de L.
; Cunha, Carlo M.
; Barboza, Carlos A. de M.
; Lucena, Carlos A.S. de
; Barreto, Carlos
; Santana, Carlos D.C.M. de
; Agne, Carlos E.Q.
; Mielke, Carlos G.C.
; Caetano, Carlos H.S.
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; Lamas, Carlos J.E.
; Rocha, Carlos
; Mascarenhas, Carolina S.
; Margaría, Cecilia B.
; Waichert, Cecilia
; Digiani, Celina
; Haddad, Célio F.B.
; Azevedo, Celso O.
; Benetti, Cesar J.
; Santos, Charles M.D. dos
; Bartlett, Charles R.
; Bonvicino, Cibele
; Ribeiro-Costa, Cibele S.
; Santos, Cinthya S.G.
; Justino, Cíntia E.L.
; Canedo, Clarissa
; Bonecker, Claudia C.
; Santos, Cláudia P.
; Carvalho, Claudio J.B. de
; Gonçalves, Clayton C.
; Galvão, Cleber
; Costa, Cleide
; Oliveira, Cléo D.C. de
; Schwertner, Cristiano F.
; Andrade, Cristiano L.
; Pereira, Cristiano M.
; Sampaio, Cristiano
; Dias, Cristina de O.
; Lucena, Daercio A. de A.
; Manfio, Daiara
; Amorim, Dalton de S.
; Queiroz, Dalva L. de
; Queiroz, Dalva L. de
; Colpani, Daniara
; Abbate, Daniel
; Aquino, Daniel A.
; Burckhardt, Daniel
; Cavallari, Daniel C.
; Prado, Daniel de C. Schelesky
; Praciano, Daniel L.
; Basílio, Daniel S.
; Bená, Daniela de C.
; Toledo, Daniela G.P. de
; Takiya, Daniela M.
; Fernandes, Daniell R.R.
; Ament, Danilo C.
; Cordeiro, Danilo P.
; Silva, Darliane E.
; Pollock, Darren A.
; Muniz, David B.
; Gibson, David I.
; Nogueira, David S.
; Marques, Dayse W.A.
; Lucatelli, Débora
; Garcia, Deivys M.A.
; Baêta, Délio
; Ferreira, Denise N.M.
; Rueda-Ramírez, Diana
; Fachin, Diego A.
; Souza, Diego de S.
; Rodrigues, Diego F.
; Pádua, Diego G. de
; Barbosa, Diego N.
; Dolibaina, Diego R.
; Amaral, Diogo C.
; Chandler, Donald S.
; Maccagnan, Douglas H.B.
; Caron, Edilson
; Carvalho, Edrielly
; Adriano, Edson A.
; Abreu Júnior, Edson F. de
; Pereira, Edson H.L.
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; Santos, Eduardo F. dos
; Shimbori, Eduardo M.
; Suárez-Morales, Eduardo
; Arruda, Eliane P. de
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; Lima, Élison F.B.
; Castro, Elizeu B. de
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; Razzolini, Emanuel
; Gama, Emanuel R.R.
; Araujo, Enilma M. de
; Nishiyama, Eric Y.
; Spiessberger, Erich L.
; Santos, Érika C.L. dos
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; Galati, Eunice A.B.
; Oliveira Junior, Evaldo C. de
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; Lansac-Tôha, Fábio A.
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; Mauro, Fabio
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; Olmos, Fabio
; Amaral, Fabio R.
; Schunck, Fabio
; Godoi, Fábio S. P. de
; Machado, Fabrizio M.
; Barbo, Fausto E.
; Agrain, Federico A.
; Ribeiro, Felipe B.
; Moreira, Felipe F.F.
; Barbosa, Felipe F.
; Silva, Fenanda S.
; Cavalcanti, Fernanda F.
; Straube, Fernando C.
; Carbayo, Fernando
; Carvalho Filho, Fernando
; Zanella, Fernando C.V.
; Jacinavicius, Fernando de C.
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; Leivas, Fernando
; Dias, Fernando M.S.
; Mantellato, Fernando
; Vaz-de-Mello, Fernando Z.
; Gudin, Filipe M.
; Albuquerque, Flávio
; Molina, Flavio B.
; Passos, Flávio D.
; Shockley, Floyd W.
; Pinheiro, Francielly F.
; Mello, Francisco de A.G. de
; Nascimento, Francisco E. de L.
; Franco, Francisco L.
; Oliveira, Francisco L. de
; Melo, Francisco T. de V.
; Quijano, Freddy R.B.
; Salles, Frederico F.
; Biffi, Gabriel
; Queiroz, Gabriel C.
; Bizarro, Gabriel L.
; Hrycyna, Gabriela
; Leviski, Gabriela
; Powell, Gareth S.
; Santos, Geane B. dos
; Morse, Geoffrey E.
; Brown, George
; Mattox, George M.T.
; Zimbrão, Geraldo
; Carvalho, Gervásio S.
; Miranda, Gil F.G.
; Moraes, Gilberto J. de
; Lourido, Gilcélia M.
; Neves, Gilmar P.
; Moreira, Gilson R.P.
; Montingelli, Giovanna G.
; Maurício, Giovanni N.
; Marconato, Gláucia
; Lopez, Guilherme E.L.
; Silva, Guilherme L. da
; Muricy, Guilherme
; Brito, Guilherme R.R.
; Garbino, Guilherme S.T.
; Flores, Gustavo E.
; Graciolli, Gustavo
; Libardi, Gustavo S.
; Proctor, Heather C.
; Gil-Santana, Helcio R.
; Varella, Henrique R.
; Escalona, Hermes E.
; Schmitz, Hermes J.
; Rodrigues, Higor D.D.
; Galvão Filho, Hilton de C.
; Quintino, Hingrid Y.S.
; Pinto, Hudson A.
; Rainho, Hugo L.
; Miyahira, Igor C.
; Gonçalves, Igor de S.
; Martins, Inês X.
; Cardoso, Irene A.
; Oliveira, Ismael B. de
; Franz, Ismael
; Fernandes, Itanna O.
; Golfetti, Ivan F.
; S. Campos-Filho, Ivanklin
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; Delabie, Jacques H.C.
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; Prando, Jadila S.
; Patton, James L.
; Bitencourt, Jamille de A.
; Silva, Janaina M.
; Santos, Jandir C.
; Arruda, Janine O.
; Valderrama, Jefferson S.
; Dalapicolla, Jeronymo
; Oliveira, Jéssica P.
; Hájek, Jiri
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; Narita, João P.
; Martin, João P.I.
; Grazia, Jocélia
; McHugh, Joe
; Cherem, Jorge J.
; Farias Júnior, José A.S.
; Fernandes, Jose A.M.
; Pacheco, José F.
; Birindelli, José L.O.
; Rezende, José M.
; Avendaño, Jose M.
; Duarte, José M. Barbanti
; Ribeiro, José R. Inácio
; Mermudes, José R.M.
; Pujol-Luz, José R.
; Santos, Josenilson R. dos
; Câmara, Josenir T.
; Teixeira, Joyce A.
; Prado, Joyce R. do
; Botero, Juan P.
; Almeida, Julia C.
; Kohler, Julia
; Gonçalves, Julia P.
; Beneti, Julia S.
; Donahue, Julian P.
; Alvim, Juliana
; Almeida, Juliana C.
; Segadilha, Juliana L.
; Wingert, Juliana M.
; Barbosa, Julianna F.
; Ferrer, Juliano
; Santos, Juliano F. dos
; Kuabara, Kamila M.D.
; Nascimento, Karine B.
; Schoeninger, Karine
; Campião, Karla M.
; Soares, Karla
; Zilch, Kássia
; Barão, Kim R.
; Teixeira, Larissa
; Sousa, Laura D. do N.M. de
; Dumas, Leandro L.
; Vieira, Leandro M.
; Azevedo, Leonardo H.G.
; Carvalho, Leonardo S.
; Souza, Leonardo S. de
; Rocha, Leonardo S.G.
; Bernardi, Leopoldo F.O.
; Vieira, Letícia M.
; Johann, Liana
; Salvatierra, Lidianne
; Oliveira, Livia de M.
; Loureiro, Lourdes M.A. El-moor
; Barreto, Luana B.
; Barros, Luana M.
; Lecci, Lucas
; Camargos, Lucas M. de
; Lima, Lucas R.C.
; Almeida, Lucia M.
; Martins, Luciana R.
; Marinoni, Luciane
; Moura, Luciano de A.
; Lima, Luciano
; Naka, Luciano N.
; Miranda, Lucília S.
; Salik, Lucy M.
; Bezerra, Luis E.A.
; Silveira, Luis F.
; Campos, Luiz A.
; Castro, Luiz A.S. de
; Pinho, Luiz C.
; Silveira, Luiz F.L.
; Iniesta, Luiz F.M.
; Tencatt, Luiz F.C.
; Simone, Luiz R.L.
; Malabarba, Luiz R.
; Cruz, Luiza S. da
; Sekerka, Lukas
; Barros, Lurdiana D.
; Santos, Luziany Q.
; Skoracki, Maciej
; Correia, Maira A.
; Uchoa, Manoel A.
; Andrade, Manuella F.G.
; Hermes, Marcel G.
; Miranda, Marcel S.
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; Monné, Marcela L.
; Labruna, Marcelo B.
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; Botelho, Marcia J.C.
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; Bologna, Marco A.
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; Boldrini, Rafael
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; Singer, Randal A.
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; Gardner, Scott L.
; Leal, Sebastián A. Muñoz
; Aloquio, Sergio
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; Lima, Sheila P.
; Gadelha, Sian de S.
; Thiengo, Silvana C.
; Cohen, Simone C.
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; Rosa, Simone P.
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; Letana, Sócrates D.
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; Martins, Thiago F.
; Alvarenga, Thiago M.
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; McElrath, Thomas C.
; Henry, Thomas
; Pikart, Tiago G.
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; Krolow, Tiago K.
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; Tomaszewska, Wioletta
; Wosiacki, Wolmar B.
; Ovando, Ximena M.C.
; Leite, Yuri L.R.
.
ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others. publications problem uptodate up date classifications context exception (CTFB http//fauna.jbrj.gov.br/, httpfaunajbrjgovbr http //fauna.jbrj.gov.br/ , jbrj gov br (http://fauna.jbrj.gov.br/) 2015 Brazil 80 specialists 1 2024 133691 133 691 133,69 125138 125 138 125,13 82.3%, 823 82 3 (82.3% 102000 102 000 102,00 7.69%, 769 7 69 (7.69% 11000 11 11,00 . 3,567 3567 567 (3,56 2,292 2292 2 292 (2,29 1,833 1833 833 (1,83 1,447 1447 447 (1,44 1000 1,00 831 (83 628 (62 606 (60 520 (52 50 users science health biology law anthropology education others http//fauna.jbrj.gov.br/ faunajbrjgovbr //fauna.jbrj.gov.br (http://fauna.jbrj.gov.br/ 201 8 202 13369 13 133,6 12513 12 125,1 82.3% (82.3 10200 10 00 102,0 7.69% 76 6 (7.69 1100 11,0 3,56 356 56 (3,5 2,29 229 29 (2,2 1,83 183 83 (1,8 1,44 144 44 (1,4 100 1,0 (8 62 (6 60 52 (5 5 http//fauna.jbrj.gov.br (http://fauna.jbrj.gov.br 20 1336 133, 1251 125, 82.3 (82. 1020 0 102, 7.69 (7.6 110 11, 3,5 35 (3, 2,2 22 (2, 1,8 18 (1, 1,4 14 4 ( 82. (82 7.6 (7. 3, (3 2, (2 (1 7. (7
2.
ROBOTIC VERSUS LAPAROSCOPIC ROUX-EN-Y-GASTRIC BYPASS: A RETROSPECTIVE STUDY IN A SINGLE CENTER ROUXENYGASTRIC ROUX EN Y GASTRIC BYPASS
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BARROS, Fernando de
; FONSECA, Ana Beatriz Monteiro
; KISS, Amanda Sebestjen Balogh
; BRAGA, Camilla Ferreira
; DA-SILVA, Filipe Roza
; REGONATI, Yumi Honda
.
ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)
- Métricas do periódico
ABSTRACT BACKGROUND: Bariatric surgery is the best treatment option for patients with obesity. As a result of the advancement of technology, the robotic gastric bypass presents promising results, despite its still high costs. AIMS: The aim of this study was to compare patients submitted to a robotic versus a laparoscopic gastric bypass at a single center by a single surgeon. METHODS: This retrospective study collected data from the medical records of 221 patients (121 laparoscopic procedures versus 100 with daVinci platform). The variables analyzed were sex, age, body mass index, comorbidities, surgical time, length of stay, and complications. RESULTS: The mean surgical time for patients in the robotic group was shorter (102.41±39.44 min versus 113.86±39.03 min, p=0.018). The length of hospital stay in robotic patients was shorter (34.12±20.59 h versus 34.93±11.74 h, p=0.007). There were no serious complications. CONCLUSIONS: The group submitted to the robotic method had a shorter surgical time and a shorter hospital stay. No difference was found regarding strictures, bleeding, or leakage. BACKGROUND obesity technology results costs AIMS surgeon METHODS 22 121 (12 10 platform. platform . platform) sex age index comorbidities complications RESULTS 102.41±39.44 102413944 102 41 39 44 (102.41±39.4 113863903 113 86 03 113.86±39.0 p=0.018. p0018 p p=0.018 0 018 p=0.018) 34.12±20.59 34122059 34 12 20 59 (34.12±20.5 34931174 93 11 74 34.93±11.7 p=0.007. p0007 p=0.007 007 p=0.007) CONCLUSIONS strictures bleeding leakage 2 (1 1 102.41±39.4 10241394 4 3 (102.41±39. 11386390 8 113.86±39. p001 p=0.01 01 34.12±20.5 3412205 5 (34.12±20. 3493117 9 7 34.93±11. p000 p=0.00 00 ( 102.41±39. 1024139 (102.41±39 1138639 113.86±39 p00 p=0.0 34.12±20. 341220 (34.12±20 349311 34.93±11 102.41±39 102413 (102.41±3 113863 113.86±3 p0 p=0. 34.12±20 34122 (34.12±2 34931 34.93±1 102.41±3 10241 (102.41± 11386 113.86± p=0 34.12±2 3412 (34.12± 3493 34.93± 102.41± 1024 (102.41 1138 113.86 p= 34.12± 341 (34.12 349 34.93 102.41 (102.4 113.8 34.12 (34.1 34.9 102.4 (102. 113. 34.1 (34. 34. 102. (102 (34 (10 (3
RESUMO RACIONAL: A cirurgia bariátrica é a melhor opção de tratamento para pacientes portadores de obesidade. Em decorrência do avanço da tecnologia, o bypass gástrico robótico apresenta resultados promissores, apesar de seus custos ainda elevados. OBJETIVOS: Comparar pacientes submetidos a bypass gástrico robótico versus laparoscópico em um único centro por um único cirurgião. MÉTODOS: Estudo retrospectivo com coleta de dados dos prontuários de 221 pacientes (121 procedimentos laparoscópicos vs 100 com plataforma daVinci). As variáveis analisadas foram sexo, idade, IMC, comorbidades, tempo cirúrgico, tempo de internação e complicações. RESULTADOS: O tempo cirúrgico médio dos pacientes do grupo robótico foi menor (102,41 ± 39,44 min. vs 113,86±39,03 min, p=0,018). O tempo de internação em pacientes robóticos foi menor (34,12±20,59 h vs 34,93±11,74 h, p=0,007). Não houve complicações graves. CONCLUSÕES: O grupo submetido ao método robótico apresentou menor tempo cirúrgico e menor tempo de internação. Nenhuma diferença foi encontrada na amostra em relação a estenoses, sangramento ou vazamento. RACIONAL obesidade tecnologia promissores elevados OBJETIVOS cirurgião MÉTODOS 22 121 (12 10 daVinci. daVinci . daVinci) sexo idade IMC comorbidades RESULTADOS 102,41 10241 102 41 (102,4 3944 39 44 39,4 min 113863903 113 86 03 113,86±39,0 p=0,018. p0018 p p=0,018 0 018 p=0,018) 34,12±20,59 34122059 34 12 20 59 (34,12±20,5 34931174 93 11 74 34,93±11,7 p=0,007. p0007 p=0,007 007 p=0,007) graves CONCLUSÕES estenoses vazamento 2 (1 1 102,4 1024 4 (102, 394 3 39, 11386390 8 113,86±39, p001 p=0,01 01 34,12±20,5 3412205 5 (34,12±20, 3493117 9 7 34,93±11, p000 p=0,00 00 ( 102, (102 1138639 113,86±39 p00 p=0,0 34,12±20, 341220 (34,12±20 349311 34,93±11 (10 113863 113,86±3 p0 p=0, 34,12±20 34122 (34,12±2 34931 34,93±1 11386 113,86± p=0 34,12±2 3412 (34,12± 3493 34,93± 1138 113,86 p= 34,12± 341 (34,12 349 34,93 113,8 34,12 (34,1 34,9 113, 34,1 (34, 34, (34 (3
3.
Analysis of ocular emergencies in a reference eye center in Brazil
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Araújo, Tiago de Assis Clemente de
; Melo, Caio Rodrigo de Oliveira
; Mendes, Larissa Paz
; Higino, Taciana Mirely Maciel
; Rocha, Camilla Silva da
; Sabino, Laura Patrícia Ferreira
; Santos, Michel Bittencourt
.
Arquivos Brasileiros de Oftalmologia
- Métricas do periódico
RESUMO Objetivo: Determinar a incidência de emergências oculares em um centro oftalmológico de referência no Brasil. Métodos: O banco de dados de prontuários da Fundação Altino Ventura, Recife, Brasil, foi analisado retrospectivamente e incluiu pacientes atendidos, entre janeiro de 2017 e janeiro de 2018, na sala de emergência oftalmológica. Foram excluídos os prontuários com dados incompletos e com quadros ambulatoriais. Apenas o primeiro atendimento na emergência foi considerado para análise. Resultados: Em um ano, 134.788 pacientes (idade média de 38,7 ± 22 anos [0-99 anos]) foram admitidos na emergência da Fundação Altino Ventura. Os diagnósticos mais frequentes foram conjuntivite (52.732 casos [37,3%]), blefarite (7.213 casos [5,1%]) e corpo estranho na córnea/conjuntiva (6.925 casos [4,9%]). Corpo estranho na córnea/conjuntiva e trauma ocular foram cerca de 8 vezes e 2 vezes mais incidente em indivíduos do sexo masculino, respectivamente (p<0,001 em ambos). Triquíase e blefarite afetaram ~2 vezes mais pacientes do sexo feminino, respectivamente (p<0,001 em ambos). Corpo estranho na córnea/conjuntiva e trauma ocular afetaram mais pacientes em idade produtiva (>15 anos), enquanto úlcera, blefarite e triquíase da córnea afetaram mais pacientes idosos. Todos os grupamentos de diagnóstico (doenças infecciosas, trauma ocular, corpos estranhos, retinopatias, doenças das pálpebras, doenças da córnea, crise glaucomatosa e doenças neurooftalmológicas) foram mais incidentes na primavera (valor de p<0,001). Conclusão: As emergências oftalmológicas mais comuns no presente estudo foram as doenças infecciosas e o corpo estranho. Porém, a incidência das emergências oculares são fluências pela faixa etária e gênero do paciente, além da época do ano.
ABSTRACT Purpose: To determine the incidence of ocular emergencies and patient profiles in a public health eye center in Brazil. Methods: The medical record database of the Fundação Altino Ventura, Recife, Brazil was retrospectively analyzed and included all patients assisted at the ophthalmic emergency room between January 2017 and January 2018. Medical records with incomplete data and outpatient complaints were excluded. For records with multiple visits, only the initial visit was considered. Results: In 1 year, 134,788 patients (mean age at admission: 38.7 ± 22 years; range: 0-99 years) were admitted at the emergency room of the Fundação Altino Ventura. The most frequent diagnoses were conjunctivitis (52,732 cases; 37.3%), blepharitis (7,213 cases; 5.1%), and corneal/conjunctival foreign body (6,925 cases; 4.9%). Corneal/conjunctival foreign body and ocular trauma had an eight- and two-fold higher incidence in male patients, respectively (both p<0.001). Female patients presented a two-fold higher incidence of trichiasis and blepharitis than males (p<0.001). Corneal/conjunctival foreign body and ocular trauma affected more patients in a productive age (>15 years), while corneal ulcers, blepharitis, and trichiasis affected more elderly patients. All diagnostic clusters (e.g., infectious diseases, ocular trauma, foreign bodies, retinopathies, eyelid disorders, corneal diseases, glaucomatous crisis, and neuroophthalmological diseases) were more common during the spring season (p<0.001). Conclusion: The most common ocular emergencies in the present study were infectious diseases and foreign body. However, the incidence of ophthalmological emergencies was influenced by the age and sex of patients, as well as the time of the year.
4.
Ozone disinfection for viruses with applications in healthcare environments: a scoping review
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IRIE, Milena Suemi
; DIETRICH, Lia
; SOUZA, Gabriela Leite de
; SOARES, Priscilla Barbosa Ferreira
; MOURA, Camilla Christian Gomes
; SILVA, Gisele Rodrigues da
; PARANHOS, Luiz Renato
.
Abstract The aim of this scoping review was to provide sufficient information about the effectiveness of ozone gas in virus inactivation of surfaces and objects under different environmental conditions. The review was performed according to the list of PRISMA SrC recommendations and the JBI Manual for Evidence Synthesis for Scoping Reviews. The review was registered in Open Science Framework (OSF). EMBASE (Ovid), Lilacs, LIVIVO, MEDLINE (PubMed), SciELO, Scopus and Web of Science were primary sources, and “gray literature” was searched in OpenGray and OpenThesis. A study was included if it reported primary data on the effect of ozone gas application for vehicle-borne and airborne virus inactivation. No language or publication date restriction was applied. The search was conduct on July 1, 2020. A total of 16,120 studies were screened, and after exclusion of noneligible studies, fifteen studies fulfilled all selection criteria. Application of ozone gas varied in terms of concentration, ozone exposure period and the devices used to generate ozone gas. Twelve studies showed positive results for inactivation of different virus types, including bacteriophages, SARS-CoV-2 surrogates and other vehicle-borne viruses. Most of the studies were classified as unclear regarding sponsorship status. Although most of the population has not yet been vaccinated against COVID-19, disinfection of environments, surfaces, and objects is an essential prevention strategy to control the spread of this disease. The results of this Scoping Review demonstrate that ozone gas is promising for viral disinfection of surfaces.
5.
Ocean Literacy, formal education, and governance: A diagnosis of Brazilian school curricula as a strategy to guide actions during the Ocean Decade and beyond
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Pazoto, Carmen Edith
; Silva, Edson Pereira
; Andrade, Luiz Antonio Botelho
; Favero, Jana Menegassi del
; Alô, Camilla Ferreira Souza
; Duarte, Michelle Rezende
.
ABSTRACT Ocean Literacy (OL) was proposed by UNESCO as a goal for the Decade of Ocean Science for Sustainable Development (Ocean Decade) aiming to (1) increase understanding of the importance of the ocean, (2) significantly influence communication on related subjects, and (3) facilitate informed and responsible decision-making about the ocean and its resources. Formal education is essential to expand the reach of OL, providing people with tools to engage in coastal and marine issues consciously and knowingly. To this end, content analysis of school curricula can help planning strategies, especially to empower citizens to implement public policies. This study assessed the extent to which OL-related terms and words are present in Brazilian curricular documents at federal (National Curriculum Parameters-PCNs and Common National Curriculum Base-BNCC) and regional (Federative Units curricular guidelines-RCs) levels. Qualitative (content analysis) and quantitative analyses (descriptive and non-parametric statistics, and multivariate analysis) were performed. The number of occurrences of OL-related words and terms were registered and counted. Nineteen words were found, totaling 797 citations (a frequency of 0.0001 in the total number of words referring to content). The number of citations were higher in BNCC-based RCs than in PCN-based RCs (Kruskal-Wallis test, p = 0.0009). Principal component analysis separated the documents into two groups, one related to BNCC-based RCs of Northeast, North and Midwest regions, with a higher number of OL related terms, and the other group with the PCN based RCs of these same regions plus those documents of Southeast and South regions (principal component 1 explaining 97.90% of the total variation and having 0.93 correlation with the total frequency of citations). General results indicated that Brazilian production on fields and themes related to OL is still concentrated in national journals, books, and booklets, thus with a limited impact. In same way although school curricula in Brazil have a larger number of topics on marine environments than do other countries, they showed heterogeneity among Federative Units, but generally with the topics still representing a very small fraction of Brazilian curricula. Thus, it is necessary to expand the contents related to the ocean and marine environments in curricula to provide students with basic knowledge about the importance and functions of these environments, as well as their conservation. Therefore, results here emphasize the need to implement OL to highlight the importance of knowledge of the oceans and enable citizens to discuss marine conservation policies and promote ocean sustainability. This study provided some strategies to increase OL in formal education and, hence, reach various stakeholders, which is fundamental to implement the United Nations Ocean Decade in Brazil and the Global South.
6.
Effect of Endodontic Sealer in Young Molars Treated by Undergraduate Students - A Randomized Clinical Trial
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Cunha, Suely Aparecida
; Soares, Carlos José
; Rosatto, Camila Maria Peres
; Vieira, João Victor Soares Mendonça
; Pereira, Renata Afonso da Silva
; Soares, Priscilla Barbosa Ferreira
; Leles, Cláudio Rodrigues
; Moura, Camilla Christian Gomes
.
Resumo A rápida progressão das lesões de cárie nos dentes molares permanentes de pacientes jovens, frequentemente resulta no tratamento endodôntico. Este ensaio clinico randomizado cego avaliou o efeito de dois cimentos endodônticos (Sealer 26 e AH Plus) na incidência de dor e reparo apical após tratamento endodôntico de dentes molares jovens e identificar fatores associados aos desfechos do tratamento. O tratamento endodôntico foi realizado por estudantes de graduação em dentes molares permanentes (n=69) de pacientes jovens (n=54) na Clínica de Extensão Endodôntica e Restauradora da Universidade Federal de Uberlândia, Brasil. Protaper Next foi usado e os dois cimentos foram distribuídos aleatoriamente nas amostras, as quais foram restauradas com resina composta direta. Dois desfechos clínicos primários - reparo apical e dor pós-operatória foram avaliados após 12 e 24 meses por operadores de forma cega. Análise longitudinal dos dados incluiu a descrição das taxas de incidência e modelo de regressão misto usando Equações de Estimativa Generalizadas (GEE). As incidências (%) do reparo apical e dos dentes assintomáticos foram respectivamente 90,5/89,3 e 96,8/90,0 nos períodos de 1 e 2 anos de acompanhamento. Ausência de reparo apical foi associada com pulpectomia insatisfatória (p=0,003) e condições periapicais (p=0,007), bem como sua interação (p=0,016). Nenhuma dessas variáveis independentes foi capaz de prever a ocorrência de dor nos períodos de acompanhamento. O tratamento endodôntico mostrou resultados satisfatórios após 2 anos. O prognóstico do reparo apical depende das condições iniciais. Nenhum efeito do cimento endodôntico foi observado. O tratamento endodôntico de molares jovens associado com restaurações em resina composta realizado por estudantes de graduação tem um papel relevante na redução do risco de perda do dente permanente.
Abstract The progression of caries in permanent molar teeth of young patients, frequently result in endodontic treatment. This randomized blinded clinical trial assessed the effect of two endodontic sealers (Sealer 26 and AH Plus) on the incidence of pain and apical repair after endodontic treatment of young molar teeth, and secondly identify factors associated with the outcomes. Endodontic treatment was performed by undergraduate students in molar teeth (n=69) of young patients (n=54) at the Endodontic and Restorative Public Extension Clinic Service. Protaper Next was used and the endodontic sealers were allocated which were restored with direct composite resin. Two primary clinical outcomes - apical repair and postoperative pain, were assessed after 12 and 24 months for blinded operators. Description of incidence rates and mixed-model regression using Generalized Estimation Equations (GEE). After 2 years 69 molar teeth of 54 patients were evaluated. No effect of the endodontic sealer was observed irrespective of period of evaluation. Apical repair incidences and asymptomatic teeth were, respectively, 90.5 and 89.3, 96.8 and 90.0% during 1 and 2 years of follow-up. Failed apical repair was associated with unsatisfactory pulpectomy (p=0.003) and periapical conditions (p=0.007) as well as their interaction (p=0.016). None of these independent variables was able to predict the occurrence of pain in both periods. Prognosis of apical repair is dependent on the initial conditions. Endodontic treatment of young molars associated with composite resin restorations performed by undergraduate students have satisfactory results after 2 years and was effective to prevent the risk of permanent teeth loss.
https://doi.org/10.1590/0103-6440202003258
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7.
Reinventing the concept of homo novus in Rome: Cicero as Horace’s role model
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RESUMO Este artigo tem por objetivo observar as semelhanças entre o uso do termo homo novus pelos autores romanos Cícero e Horácio, com uma conotação positiva, em contraste com a visão do outsider como um elemento negativo. O que pretendemos sugerir é que Horácio pode ter se utilizado de elementos das obras de Cícero como forma de sustentar uma defesa de sua própria condição na sociedade romana.
ABSTRACT This paper identifies similarities between Horace’s and Cicero’s use of the termHomo novusas a positive connotation, which contrasts with the then prevailing view of the outsider as a negative element. We suggest that Horace might have drawn on elements from Cicero’s works to support the defense of his position within Roman society.
RESUMEN Este artículo tiene por objetivo observar las semejanzas entre el uso del término homo novus por los autores romanos Cícero y Horacio, con una connotación positiva, en contraste con la visión del outsider como un elemento negativo. Lo que pretendemos sugerir es que Horacio puede haber utilizado elementos de las obras de Cícero como forma de mantener una defensa de su propia condición en la sociedad romana.
https://doi.org/10.1590/2237-101x02104503
714 downloads
8.
Horácio e o uso poético-político do passado
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RESUMO O artigo analisa como o poeta Horácio se apropriou da tradição retórico-poética romana e grega de forma a estabelecer sua posição frente à sociedade romana, no contexto de alargamento de fronteiras e de fabricação de um novo regime político, o Principado de Augusto. O passado, sempre atualizado de acordo com as circunstâncias, é redefinido por Horácio na defesa de seu status, utilizando a sua vitoriosa carreira como um cursus poetarum em espelhamento ao cursus honorum, o que lhe conferia autoridade para proferir conselhos à elite imperial nascente bem como se dirigir a figuras poderosas. Ao longo do século I a.C., muitos novi homines, como Cícero e o próprio Horácio, ascenderam socialmente, sobretudo por sua atuação política e econômica. Como tais homens não possuíam um passado louvável no qual poderiam se apoiar para se estabelecerem política e socialmente, empregaram estratégias retóricas para reescreverem o passado, utilizando-o como ferramenta para se fortalecerem no jogo do poder.
ABSTRACT In this paper, we analyze how Horace, the Roman poet, took on the Greek and Roman rhetorical and poetical traditions in order to establish his own social role, within a context of widening of boundaries and fabrication of a new political regime, the Augustan Principate. The past, always updated according to circumstances, is redefined by Horace in defense of his status, by using his successful career as his cursus poetarum, mirroring the cursus honorum, to confer him authority to advise the newborn imperial elite, as well as converse with powerful figures. Throughout the first century BC, many novi homines, such as Cicero and Horace himself, socially ascended, mainly due to their political and economic performance. As these men did not have a laudable past on which they could rely to establish themselves socially and politically, they employed rhetorical strategies to rewrite the past, using it as a tool to strengthen their own sides in the game of power.
https://doi.org/10.1590/1806-93472020v40n84-04
859 downloads
9.
Rompimento da barragem em Brumadinho: um relato de experiência sobre os debates no processo de desastres
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Costa, Giulia Balbi Rodrigues da
; Lau, Geórgia Rolemberg
; Silva, Camilla Ferreira da
; Mantel, Maria Clara Barroso
; Peres, Maria Cristina Mitsuko
; Luna, Tatiane Nunes da Silva Santos
; Silva, Priscila Neves da
.
RESUMO O presente artigo teve como objetivo aprofundar discussões do desastre ocorrido em Brumadinho (MG), em janeiro de 2019. A partir da relatoria do seminário ‘Desastre da Vale S.A. em Brumadinho: seis meses de impacto e ações’, seis alunas do Curso de Especialização em Saúde Pública da Fundação Oswaldo Cruz (Fiocruz) e uma pós-doutoranda da Fiocruz de Minas Gerais realizaram transcrições de falas feitas durante o evento na tentativa de reportar essa vivência mediante lugares de fala distintos. Experiências artísticas retratadas ao longo do texto estabelecem um vínculo com a subjetividade dos atingidos e seu entorno. Portanto, o artigo pretendeu seguir esse caminho.
ABSTRACT The purpose of this article was to deepen discussions on the disaster that occurred in Brumadinho (MG), in January 2019. From the report of the seminar ‘Disaster of Vale S.A. in Brumadinho: six months of impact and actions’, six students from the Specialization Course in Public Health at Oswaldo Cruz Foundation (Fiocruz) and a postdoctoral fellow at Fiocruz in Minas Gerais performed transcriptions of speeches made during the event in an attempt to report this experience through different places of speech. Artistic experiences portrayed throughout the text establish a link with the subjectivity of those affected and their surroundings. Therefore, the article intended to follow that path.
10.
Rompimento da barragem em Brumadinho: um relato de experiência sobre os debates no processo de desastres
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Costa, Giulia Balbi Rodrigues da
; Lau, Geórgia Rolemberg
; Silva, Camilla Ferreira da
; Mantel, Maria Clara Barroso
; Peres, Maria Cristina Mitsuko
; Luna, Tatiane Nunes da Silva Santos
; Silva, Priscila Neves da
.
RESUMO O presente artigo teve como objetivo aprofundar discussões do desastre ocorrido em Brumadinho (MG), em janeiro de 2019. A partir da relatoria do seminário ‘Desastre da Vale S.A. em Brumadinho: seis meses de impacto e ações’, seis alunas do Curso de Especialização em Saúde Pública da Fundação Oswaldo Cruz (Fiocruz) e uma pós-doutoranda da Fiocruz de Minas Gerais realizaram transcrições de falas feitas durante o evento na tentativa de reportar essa vivência mediante lugares de fala distintos. Experiências artísticas retratadas ao longo do texto estabelecem um vínculo com a subjetividade dos atingidos e seu entorno. Portanto, o artigo pretendeu seguir esse caminho.
ABSTRACT The purpose of this article was to deepen discussions on the disaster that occurred in Brumadinho (MG), in January 2019. From the report of the seminar ‘Disaster of Vale S.A. in Brumadinho: six months of impact and actions’, six students from the Specialization Course in Public Health at Oswaldo Cruz Foundation (Fiocruz) and a postdoctoral fellow at Fiocruz in Minas Gerais performed transcriptions of speeches made during the event in an attempt to report this experience through different places of speech. Artistic experiences portrayed throughout the text establish a link with the subjectivity of those affected and their surroundings. Therefore, the article intended to follow that path.
https://doi.org/10.1590/0103-11042020e226
170 downloads
11.
Defining the reproductive period of freshwater fish species using the Gonadosomatic Index: a proposed protocol applied to ten species of the Patos Lagoon basin
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Fontoura, Nelson Ferreira
; Ceni, Gianfranco
; Braun, Aloisio Sirangelo
; Marques, Camilla da Silva
.
RESUMO Esta pesquisa registra o período reprodutivo de dez espécies de peixes dulcícolas dominantes na região límnica da Laguna dos Patos e Lago Guaíba (Astyanax fasciatus, Cyphocharax voga, Hoplias malabaricus, Oligosarcus jenynsii, Oligosarcus robustus, Hoplosternum littorale, Loricariichthys anus, Parapimelodus nigribarbis, Trachelyopterus lucenai, Pachyurus bonariensis). Os dados derivam de amostras mensais realizadas na Lagoa do Casamento (Nordeste da Laguna dos Patos; Nov. 2002 a Abr. 2004) e no Lago Guaíba (Jun. 2005 a Maio 2006). A reprodução de peixes foi identificada durante todos os meses do ano. Oligosarcus jenynsii iniciou a reprodução no inverno, mas prolongou a desova até a primavera (reprodução no início do ciclo de aquecimento da água). Três espécies também apresentaram reprodução durante os meses de aquecimento da água, mas começando na primavera e terminando no verão (reprodução tardia do ciclo de aquecimento da água): P. nigribarbis, T. lucenai e P. bonariensis. Três espécies apresentaram períodos de reprodução relativamente curtos no verão (reprodução concentrada em período de água quente): H. malabaricus, H. littorale e L. anus, e apenas uma espécie se reproduz quase continuamente durante águas mais quentes (reprodução de longa duração em período de água quente): A. fasciatus. Finalmente, duas outras espécies apresentaram um padrão reprodutivo muito distinto, iniciando a reprodução no final do verão, mas aumentando os valores do IGS ao longo do outono e inverno (reprodutores de longo período com águas em resfriamento): C. voga e O. robustus.
ABSTRACT This contribution records the reproductive periods of ten dominant freshwater fish species from the Patos Lagoon and Guaíba Lake (Astyanax fasciatus, Cyphocharax voga, Hoplias malabaricus, Oligosarcus jenynsii, Oligosarcus robustus, Hoplosternum littorale, Loricariichthys anus, Parapimelodus nigribarbis, Trachelyopterus lucenai, Pachyurus bonariensis). Data were derived from monthly samples in Casamento Lake (northern Patos Lagoon; Nov. 2002 to Apr. 2004) and Guaíba Lake (Jun. 2005 to May 2006). The reproductive period was determined according to the monthly variation of the gonadosomatic index (GSI). Fish reproduction was identified during all months of the year. Oligosarcus jenynsii started reproduction in winter, but extended spawning to spring (early warming-water reproduction). Three species also presented reproduction during warming water months, but beginning in spring and finishing in summer (late warm-water reproduction): P. nigribarbis, T. lucenai and P. bonariensis. Three species presented relatively short reproduction periods on summer (spotted warm-water reproduction): H. malabaricus, H. littorale and L. anus, and only one species reproduces almost continuously during warmer waters (long-season warm-water reproduction): A. fasciatus. Finally, two other species presented a very distinct reproductive pattern, starting reproduction on late summer but increasing GSI values along autumn and winter (long-season cooling-water reproducers): C. voga and O. robustus.
https://doi.org/10.1590/1982-0224-20170006
1600 downloads
12.
Prediction equations for energy values of animal meals obtained using meta-analysis
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Oliveira, Camilla Roana Costa de
; Rabello, Carlos Bôa-Viagem
; Souza, Janete Gouveia de
; Nascimento, Guilherme Rodrigues do
; Lopes, Elainy Cristina
; Silva, Almir Ferreira da
; Silva, Jaqueline de Cássia Ramos da
; Macambira, Gabriel Miranda
.
ABSTRACT The objective of this study was to determine prediction equations to estimate the nitrogen-corrected apparent metabolizable energy (AMEn) values of animal meals used in broiler diets through meta-analysis. A bibliographic review was undertaken with studies conducted in Brazil from 2000 to 2016 to catalogue information on AMEn values and the following chemical elements in the composition of the feedstuffs: crude protein (CP), ether extract (EE), gross energy (GE), mineral matter (MM), calcium (Ca), and phosphorus (P). Groups were also catalogued and formed according to sex and age of birds. Chemical correlations were analyzed, and a multiple linear regression model with the stepwise procedure was used to examine the association between the variables, which were included in the equation as a function of their importance. High and significant correlation coefficients between the independent (GE, MM, CP, EE, Ca, and P) and dependent variable (AMEn) contribute to the understanding of variations in the energy values of these feedstuffs. According to the coefficients of determination, the best equations to estimate AMEn of poultry offal meal and meat and bone meal are AMEn = 6139 − 45.5 CP + 0.356 GE − 123.5 MM ( R 2 = 0.8302 ) and AMEn = 2267 + 19.9 CP + 67.9 EE − 44.4 MM ( R 2 = 0.9021 ) , respectively.
https://doi.org/10.1590/rbz4720180098
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13.
The use of different indicators for interpreting the local knowledge loss on medical plants
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Brito, Camilla de Carvalho de
; Silva, Taline Cristina da
; Albuquerque, Ulysses Paulino
; Ramos, Marcelo Alves
; Ferreira Júnior, Washington Soares
; Barros, Fernanda Novais
; Costa Neto, Eraldo Medeiros
; Medeiros, Patrícia Muniz de
.
Revista Brasileira de Farmacognosia
- Métricas do periódico
ABSTRACT The increasing loss of local ecological knowledge may have negative impacts on the resilience of socio-ecological systems and may also negatively impact bioprospecting efforts, since local ecological knowledge is an important source of information for searching new drugs. Recent studies try to evaluate whether communities are experiencing loss of local ecological knowledge. However, some of them make conclusions which are erroneously based on specific analyses of a single indicator. We propose an integrative analysis of three indicators, namely: number of plants cited by young people and elders, therapeutic choices and people's connectance in terms of medicinal plant learning. The study was carried out in the community of Sucruiuzinho (Bahia, Brazil). We conducted semistructured interviews and a therapeutic recall with 24 local dwellers. We did not find evidence of local ecological knowledge loss in the studied community. Although younger people know fewer plants, they are well connected in terms of knowledge transmission. Moreover, in illness events, young people and adults have similar proportions of choice for plants when compared to allopathy. Concomitant use of the three indicators leads to a more realistic scenario of local ecological knowledge loss than the use of only one of them.
https://doi.org/10.1016/j.bjp.2016.09.006
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14.
Biological Effects of a Root Conditioning Treatment on Periodontally Affected Teeth - An In Vitro Analysis
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Silva, Aline Cristina
; Moura, Camilla Christian Gomes
; Ferreira, Jessica Afonso
; Magalhães, Denildo de
; Dechichi, Paula
; Soares, Priscilla Barbosa Ferreira
.
Resumo O objetivo deste estudo foi avaliar modificações nas superfícies radiculares sofridas pela aplicação de diferentes agentes químicos, e sua influência sobre a rede de fibrina e adesão de fibroblastos. A partir de 96 incisivos inferiores humanos, 192 blocos de dentina das superfícies vestibular e lingual foram obtidos e divididos aleatoriamente em 6 grupos: Cont-grupo controle, não recebeu nenhum tratamento; Raspagem e alisamento radicular (RAR); Ácido cítrico-SRP; EDTA-SRP; Tetraciclina em cápsula-SRP; Tetraciclina gel-SRP. Após o tratamento da dentina as espécimes foram analisadas: 1, nível de desmineralização e resíduos do produto por microscopia eletrônica de varredura (MEV); 2, adesão dos componentes sanguineos após 20 min na tratamento de superfície por SEM; 3, adesão de fibroblastos após 24h por SEM; 4, o metabolismo celular após 24 h por ensaio MTT. Os dados foram analisados por Fisher Exact, teste one-way ANOVA, seguido pelo teste de Dunn, teste de Tukey e teste de Dunnett (α = 0,05). O ácido cítrico, EDTA e gel tetraciclina resultaram na adequada desmineralização sem remoção completa de camada de smear layer e smear plug sobre a superfície da dentina radicular. Cápsula de tetraciclina produziu grandes resíduos de tetraciclina com várias áreas de desmineralização. Os grupos Gel de tetraciclina e EDTA apresentaram maior adesão de fibroblastos do que os demais grupos experimentais. O maior score de adesão de coágulo sanguineo foi observado nas superfícies tratadas com gel de tetraciclina. EDTA e Gel de tetraciclina removeram a camada de smear layer sobre a superfície da dentina e promoveu adesão da rede de fibrina e de fibroblastos.
Abstract The aim of this study was to evaluated the root surfaces modifications resulted by application of different chemicals agents, and their influence on the fibrin network and fibroblasts attachment. From 96 anterior mandibular human extracted incisor teeth, 192 dentin blocks of buccal and lingual surface were obtained and randomly divided into 6 groups: Cont- control group, which received no treatment; Root surface scaling and root planing (Srp); Citric acid-Srp; EDTA-Srp; Tetracycline capsule-Srp; Tetracycline gel-Srp. After dentin treatments the specimens were analyzed as follows: 1) demineralization level and residues of the product by scanning electron microscopy (SEM); 2) adhesion of blood components after 20 min of surface treatment by SEM; 3) fibroblast attachment after 24 h by SEM; 4) cell metabolism after 24 h by MTT assay. Data were analyzed using Fisher Exact, One-way ANOVA test followed by Dunn's test, Tukey test and Dunnett test (α=0.05). Citric acid, EDTA and Tetracycline gel resulted in adequate demineralization with no completely smear layer and smear plug removal on root dentin surface. Tetracycline capsule produced great tetracycline residues with several demineralization areas. Tetracycline gel and EDTA groups presented more fibroblast fixation than other experimental groups. The highest mean blood clot adhesion score was observed in roots treated with tetracycline gel. EDTA and Tetracycline gel surface treatment removed the smear layer over dentin surface and promoted adhesion of fibrin network and fibroblast cells attachment.
https://doi.org/10.1590/0103-6440201600427
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15.
Effect of lectin (ScLL) on fibroblasts stimulated with LPS - an in vitro study
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REIS, Manuella Verdinelli de Paula
; MOURA, Camilla Christian Gomes
; SILVA, Marcus Vinicius da
; SOUZA, Maria Aparecida de
; SOARES, Priscilla Barbosa Ferreira
; SOARES, Carlos José
.
Abstract: The lectin (ScLL) extracted from the Synadenium carinatum plant has been evaluated as an immunomodulator in diseases such as asthma, neosporosis and leishmaniasis. However, it has not yet been evaluated in the oral cavity. This study evaluated the effect of ScLL on viability, proliferation and release of IL-10 in human gingival fibroblasts (HGF) stimulated with lipopolysaccharide (LPS). HGF were stimulated with LPS 1 µg/ml and treated with ScLL in concentrations of 10, 5 and 2 µg/ml for 1 and 5 h, and evaluated by flow cytometry for viability, apoptosis (initial/advanced) and necrosis. The supernatant was collected to detect release of IL-10 by ELISA. The proliferation was assessed with the BrdU assay. Positive control consisted of cells maintained in Dulbecco's Modified Eagles Medium (DMEM), and the negative control, of those kept in tap water. Data were analyzed by ANOVA and Dunnett's test (α = 0.05). No significant difference was found for ScLL concentrations regarding viability or initial and advanced apoptosis (p=0.455). All the groups, including the positive control, had a significantly lower necrosis parameter than negative control at 5 h (p < 0.001). No difference was found for proliferation among the experimental groups (p = 0.832). ScLL at 5 and 2 µg/ml resulted in a lower release of IL-10 than positive and negative controls at 5 h (p = 0.047). The results indicated that ScLL concentrations tested were not cytotoxic, and had no effect on proliferation and release of IL-10 parameters. A thorough understanding of ScLL, regarding its immunomodulatory potential, may open the door to new perspectives for dentistry.
https://doi.org/10.1590/1807-3107bor-2016.vol30.0140
1051 downloads
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