Abstract Introduction Williams syndrome (WS) is a genetic disorder caused by a microdeletion in chromosome 7, affecting ~ 28 genes. Studies have demonstrated conductive losses seemingly related to the absence of the elastin gene and mild to profound sensorineural losses due to cochlear fragility. Objective To characterize and compare the peripheral auditory system and auditory brainstem response (ABR) of adults with WS and neurotypical adults matched by age and gender. Methods We conducted a cross-sectional observational study with 30 individuals of both sexes, aged 18 to 37 years – 15 of them with WS (study group) and 15 with neither the syndrome nor hearing complaints (control group), matched for sex and age. The subjects underwent pure-tone and speech audiometry, acoustic immittance, transient-evoked otoacoustic emissions (TEOAEs), and ABR. Results Early-onset sensorineural hearing loss was found in 53.3% of the study sample, mostly mild, occurring above 3 kHz. The TEOAEs were absent in 53.3% of assessed subjects; for those in whom they were present, the signal-to-noise responses were significantly lower than in the control group. In the ABR, increased absolute latencies were observed in waves I and III. Conclusion Individuals with WS have early and progressive cochlear impairments, mainly affecting the basal region of the cochlea. They may have low brainstem changes which seem to begin in adulthood. (WS 7 2 genes fragility ABR (ABR gender crosssectional cross sectional sexes 1 group group, , puretone pure tone audiometry immittance transientevoked transient evoked TEOAEs, (TEOAEs) Earlyonset Early onset 533 53 53.3 sample kHz present signaltonoise signal noise III impairments cochlea adulthood (TEOAEs 5 53.

RESUMEN Objetivo: Realizar la adaptación transcultural de la escala de Pasero Opioid-induced Sedation Scale para el contexto brasileño. Método: Estudio metodológico, utilizando el marco de referencia de Beaton, que consta de seis fases: traducción, síntesis de traducciones, traducción inversa, comité de expertos, prueba previa, y envío del instrumento adaptado ala autora del instrumento original. El estudio se realizó de abril a diciembre de 2021, y a la investigación se condujo en un hospital privado, ubicado en la ciudad de São Paulo, en las unidades de hospitalización de adultos y cuidados críticos. Recibió la aprobación del comité de ética. Resultados: Después de las etapas de traducción, síntesis de traducción y traducción inversa, la versión fue evaluada por un comité de expertos, con dos rondas para obtener valores aceptables de índice de validez de contenido superiores a 0,80. En la fase previa a la prueba, la escala mostró una buena comprensión, con una puntuación de 0,98. Conclusión: La escala fue adaptada para el contexto brasileño, sin embargo, serán necesarios más estudios para analizar las evidencias de validez y confiabilidad. Objetivo Opioidinduced Opioid induced brasileño Método metodológico Beaton fases traducciones inversa expertos original 2021 privado Paulo críticos ética Resultados 080 0 80 0,80 comprensión 098 98 0,98 Conclusión embargo confiabilidad 202 08 8 0,8 09 9 0,9 20 0, 2

ABSTRACT Objective: Perform a cross-cultural adaptation of the Pasero Opioid-induced Sedation Scale to the Brazilian setting. Method: This is a methodological study using Beaton’s framework, which consists in six stages: translation, synthesis of translations, re-translation, expert committee, pre-test, and sending the adapted version of the instrument to the author of the original. The study was carried out from April to December 2021. The research was conducted in a private hospitalin the city of São Paulo, in the adult hospitalization and critical care units. It was approved by the research ethics committee. Results: After translation, translation synthesis and back-translation steps, the version was evaluated by the expert committee, requiring two rounds to obtain acceptable CVI values above 0.80. In the pre-test phase, the scale was well understood, with a CVI of 0.98. Conclusion: The scale was adapted for the Brazilian context; however, further studies will be needed to analyze validity and reliability evidence. Objective crosscultural cross cultural Opioidinduced Opioid induced setting Method Beatons Beaton s framework stages translations retranslation, retranslation re re-translation committee pretest, pretest pre test, test original 2021 Paulo units Results backtranslation back steps 080 0 80 0.80 phase understood 098 98 0.98 Conclusion context however evidence 202 08 8 0.8 09 9 0.9 20 0. 2

RESUMO Objetivo: Realizar adaptação transcultural da escala Pasero Opioid-Induced Sedation para o cenário brasileiro. Método: Estudo metodológico, utilizado referencial de Beaton, composto por seis fases: tradução, síntese das traduções, retradução, comitê de especialistas, pré-teste e envio dos instrumentos adaptados ao autor do instrumento original. Estudo foi realizado de abril a dezembro de 2021. A pesquisa desenvolveu-se em um hospital privado, localizado no município de São Paulo, nas unidades de internação e críticas adultos. Recebeu aprovação do comitê de ética. Resultados: Após as etapas de tradução, síntese de tradução e retrotradução a versão foi avaliada pelo comitê de especialistas, com duas rodadas para obtenção de valores aceitáveis de índice de validade de conteúdo acima de 0,80. Na fase de pré-teste a escala apresentou boa compreensão com score de 0,98. Conclusão: A escala foi adaptada para o contexto brasileiro, no entanto, novos estudos serão necessários para análises de evidências de validade e confiabilidade. Objetivo OpioidInduced Opioid Induced brasileiro Método metodológico Beaton fases traduções retradução especialistas préteste pré teste original 2021 desenvolveuse desenvolveu se privado Paulo adultos ética Resultados 080 0 80 0,80 098 98 0,98 Conclusão entanto confiabilidade 202 08 8 0,8 09 9 0,9 20 0, 2

RESUMEN Objetivo: verificar el impacto de las estrategias educativas digitales en la adherencia de los profesionales de enfermería a la identificación de pacientes, así como la aceptación de estas estrategias por parte de los profesionales. Método: estudio piloto pre y posintervención desarrollado en un hospital público docente de una ciudad del interior del estado de São Paulo, Brasil, realizado entre marzo y septiembre de 2022, en tres fases: 1) preintervención - auditorías del brazalete de identificación a pie de cama, elaboración y planificación de estrategias educativas; 2) intervención - formación en línea de corta duración ofrecida a profesionales de enfermería; 3) posintervención - repetición de auditorías. Para el análisis comparativo entre fases, se utilizó la prueba chi-cuadrado de Pearson y la prueba exacta de Fisher con un nivel de significancia del 5%. Resultados: 166 camas fueron auditadas con un alto índice de adherencia al uso de brazaletes de identificación, de acuerdo con las normas, y la presencia de placas de identificación (> 80%) tanto en el período pre como posintervención, con un aumento significativo en pacientes que identificaron que los profesionales revisaban la pulsera (p=0,0410) durante la asistencia. Los participantes evaluaron positivamente las estrategias utilizadas, aunque se registró baja adherencia al entrenamiento. Conclusión: hubo un impacto positivo en la adherencia a la identificación de pacientes, pero serán necesarias estrategias de gestión y motivación que permitan acciones futuras para implementar la capacitación en línea. Objetivo Método Paulo Brasil 2022 fases 1 cama 2 3 chicuadrado chi cuadrado 5 5% Resultados 16 normas > ( 80% 80 p=0,0410 p00410 p 0 0410 (p=0,0410 asistencia utilizadas entrenamiento Conclusión 202 8 p=0,041 p0041 041 (p=0,041 20 p=0,04 p004 04 (p=0,04 p=0,0 p00 (p=0,0 p=0, p0 (p=0, p=0 (p=0 p= (p= (p

ABSTRACT Objective: to verify the impact of digital educational strategies on nursing professionals’ adherence to patient identification as well as the acceptance of these strategies by professionals. Method: this is a pre and post-intervention pilot study developed in a public teaching hospital in a city in the countryside of the state of São Paulo, Brazil, carried out between March and September 2022, in three phases: 1) pre-intervention - audits of identification wristband at the bedside, elaboration and planning of educational strategies; 2) intervention - short-term online training offered to nursing professionals; 3) post-intervention - repeat audits. For comparative analysis between phases, Pearson’s chi-square test and Fisher’s exact test were used with a significance level of 5%. Results: a total of 166 beds were audited with a high rate of adherence to the use of identification wristbands, in accordance with standards, and the presence of identification plates (> 80%) both in the pre- and post-intervention period, with a significant increase in patients who identified that professionals checked wristband (p=0.0410) during care. Participants positively assessed the strategies used, although low adherence to training was recorded. Conclusion: there was a positive impact on adherence to patient identification, but management and motivational strategies will be necessary to enable future actions to implement online training. Objective Method postintervention post Paulo Brazil 2022 phases 1 preintervention bedside 2 shortterm short term 3 Pearsons Pearson s chisquare chi square Fishers Fisher 5 5% Results 16 wristbands standards > ( 80% 80 period p=0.0410 p00410 p 0 0410 (p=0.0410 care recorded Conclusion 202 8 p=0.041 p0041 041 (p=0.041 20 p=0.04 p004 04 (p=0.04 p=0.0 p00 (p=0.0 p=0. p0 (p=0. p=0 (p=0 p= (p= (p

RESUMO Objetivo: verificar o impacto de estratégias educacionais digitais na adesão dos profissionais de enfermagem à identificação do paciente, assim como a aceitação destas estratégias por parte dos profissionais. Método: estudo piloto de pré e pós-intervenção desenvolvido em um hospital de ensino público de uma cidade do interior do estado de São Paulo, Brasil, realizado entre março e setembro do ano de 2022, em três fases: 1) pré-intervenção - auditorias da pulseira de identificação à beira-leito, elaboração e planejamento das estratégias educacionais; 2) intervenção - treinamento online de curta duração oferecido aos profissionais de enfermagem; 3) pós-intervenção - repetição de auditorias. Para análise comparativa entre as fases, foi utilizado o teste qui-quadrado de Pearson e o teste exato de Fisher com nível de significância de 5%. Resultados: foram auditados 166 leitos com alta taxa de adesão ao uso de pulseira de identificação, de acordo com as normas, e a presença de placas de identificação (> 80%) tanto no período pré quanto pós-intervenção, com aumento significativo dos pacientes que identificaram que os profissionais conferiam a pulseira (p=0,0410) durante a assistência. Os participantes avaliaram positivamente as estratégias utilizadas, embora tenha sido registrada baixa adesão ao treinamento. Conclusão: houve um impacto positivo na adesão à identificação do paciente, porém estratégias gerenciais e motivacionais serão necessárias para viabilizar ações futuras de implementação do treinamento online. Objetivo paciente Método pósintervenção pós Paulo Brasil 2022 fases 1 préintervenção beiraleito, beiraleito beira leito, leito beira-leito 2 3 quiquadrado qui quadrado 5 5% Resultados 16 normas > ( 80% 80 pósintervenção, intervenção, p=0,0410 p00410 p 0 0410 (p=0,0410 assistência utilizadas Conclusão 202 8 p=0,041 p0041 041 (p=0,041 20 p=0,04 p004 04 (p=0,04 p=0,0 p00 (p=0,0 p=0, p0 (p=0, p=0 (p=0 p= (p= (p

Abstract: This paper presents an overview of the themes and contents covered in Astronomy Education Research conducted in postgraduate programs in Brazil between 1973 and 2018. The researchers used Content Analysis to deal with 490 studies and identify the most discussed themes and their correlation with different stages of education, including Basic Education, Higher Education, and Non-School Education. The results showed that the Sun-Earth-Moon system is the most discussed theme in Elementary and Secondary Education, while Celestial Mechanics and gravity are more prevalent in Higher Education. Research on Non-School Education often does not focus on specific themes. The researchers discovered a central focus on teaching and learning processes, reflected in the emphasis on content and method. The study identifies trends and gaps that can contribute to developments in this field. Abstract 197 2018 49 education NonSchool Non School SunEarthMoon Sun Earth Moon processes method field 19 201 4 1 20 2

Resumo: Esta pesquisa, do tipo Estado da Arte, apresenta um recorte sobre temas e conteúdos abordados nos estudos sobre Educação em Astronomia produzidos em cursos de pós-graduação no Brasil, de 1973 a 2018. Foram recuperados e analisados 490 estudos, por meio da ferramenta metodológica Análise de Conteúdo. Identificados os mais abordados, correlaciona-se a abordagem com as diferentes etapas da Educação Básica, com o Ensino Superior e com a Educação Não Escolar. O Sistema Sol-Terra-Lua destaca-se no Ensino Fundamental e Médio, enquanto no Ensino Superior, observa-se a predominância de estudos em Mecânica Celeste e Gravitação. As pesquisas na Educação Não Escolar, em geral, não se dedicam a temas específicos. A correlação entre Temas e Conteúdos e o Foco Temático têm ênfase em Conteúdo e Método, refletindo atenção central nos processos de ensino e aprendizagem. Desse modo, indica-se tendências e lacunas com o propósito de contribuir com o desenvolvimento da área. Resumo pesquisa Arte pósgraduação pós graduação Brasil 197 2018 49 correlacionase correlaciona Básica Escolar SolTerraLua Sol Terra Lua destacase destaca Médio observase observa Gravitação geral específicos Método aprendizagem modo indicase indica área 19 201 4 1 20 2

Abstract Introduction The time elapsed from diagnosis to hematopoietic stem cell transplantation (HSCT) is influenced by numerous factors. In Brazil, patients using the public health system are also dependent on the availability of HSCT-specific beds in the hematology ward. Objective and Methods We conducted a cohort study of listed patients who underwent allogeneic HSCT at a Brazilian public hospital to investigate the impact of the waitlist time on post-HSCT survival. Results The median time from diagnosis to HSCT was 19 months (IQR, 10 - 43), of which 6 months (IQR, 3 - 9) were spent on the waitlist. The time on the waitlist for HSCT appeared to influence mainly the survival of adult patients (≥ 18 years), with an increasing risk according to this time (RR, 3.53 and 95%CI, 1.81 - 6.88 for > 3 and ≤ 6 months; RR 5.86 and 95%CI, 3.26 - 10.53 for > 6 and ≤ 12 months, and; RR 4.24 and 95%CI, 2.32 - 7.75 for > 12 months). Conclusion Patients who remained on the waitlist for less than 3 months had the highest survival (median survival, 856 days; IQR, 131 - 1607). The risk of reduced survival was about 6-fold higher (95%CI, 2.8 - 11.5) in patients with malignancies. (HSCT factors Brazil HSCTspecific specific ward postHSCT post 1 IQR (IQR 43, 43 , 43) 9 ≥ ( years, years years) RR, (RR 353 53 3.5 95CI CI 95 95%CI 181 81 1.8 688 88 6.8 586 5 86 5.8 326 26 3.2 1053 10.5 424 4 24 4.2 232 2 32 2.3 775 7 75 7.7 months. . months) 85 days 13 1607. 1607 1607) 6fold fold (95%CI 28 8 2. 11.5 115 11 malignancies 35 3. 1. 68 6. 58 5. 105 10. 42 4. 23 77 7. 160 11. 16

Abstract The study analyzed the consumption of non-standard medication in the health of indigenous peoples, emphasizing the rationality of pharmacotherapy, by conducting a cross-sectional study of secondary data from 2018 and 2019 in the Minas Gerais/Espírito Santo Special Sanitary Indigenous Health Districts. These medicines were classified by Anatomical Therapeutic Chemical Classification. Non-parametric tests were applied to compare the origin of prescription and the form of acquisition, assessing access to medication. Rationality was verified through the consumption profile and the therapeutic option in the list of standardized medicines. A total of 104,928 pharmaceutical presentations were consumed, 66,967 (66%) for the alimentary tract and metabolism, 17,705 (17%) for the nervous system, and 12,961 (12%) for the cardiovascular system. With respect to medicines consumed per region, 171 (90%) out of 190 had a therapeutic option. Prescriptions were more from the SUS. Differences were found in the way the medicines were acquired. The study pointed to significant consumption of non-standard medicines, and there may be failings in therapeutic rationality. In indigenous health, ethnocultural and social issues are challenges to access to medicines with rational use. nonstandard non standard peoples pharmacotherapy crosssectional cross sectional 201 GeraisEspírito Gerais Espírito Districts Classification Nonparametric Non parametric acquisition 104928 104 928 104,92 66967 66 967 66,96 66% (66% metabolism 17705 17 705 17,70 17% (17% system 12961 12 961 12,96 12% (12% region 90% 90 (90% 19 SUS acquired use 20 10492 10 92 104,9 6696 6 96 66,9 (66 1770 1 70 17,7 (17 1296 12,9 (12 9 (90 2 1049 104, 669 66, (6 177 7 17, (1 129 12, (9 (

Resumo O estudo analisou o consumo de medicamentos não padronizados na saúde indígena, enfatizando a racionalidade da farmacoterapia, por meio de um estudo transversal dos dados secundários, de 2018 e 2019, no Distrito Especial Sanitário de Saúde Indígena Minas Gerais/Espírito Santo. Esses medicamentos foram classificados pela Anatomical Therapeutic Chemical Classification. Para a comparação da origem de prescrição e da forma de aquisição, empregaram-se testes não paramétricos, avaliando o acesso a medicamentos. Verificou-se a racionalidade por meio do perfil de consumo e da opção terapêutica na lista de medicamentos padronizados. Foram consumidas 104.928 apresentações farmacêuticas, 66.967 (66%) eram para o trato alimentar e o metabolismo; 17.705 (17%) para o sistema nervoso; 12.961 (12%) para o sistema cardiovascular. Quanto aos medicamentos mais consumidos por regiões, 171 (90%) dos 190 apresentavam opção terapêutica. As prescrições foram mais provenientes do SUS. Encontraram-se diferenças na forma de aquisição dos medicamentos. O estudo apontou importante consumo de medicamentos não padronizados, podendo existir falhas na racionalidade terapêutica. Na saúde indígena, questões etnoculturais e sociais constituem desafios para o acesso aos medicamentos com uso racional. indígena farmacoterapia secundários 201 2019 GeraisEspírito Gerais Espírito Santo Classification empregaramse empregaram se paramétricos Verificouse Verificou 104928 104 928 104.92 farmacêuticas 66967 66 967 66.96 66% (66% metabolismo 17705 17 705 17.70 17% (17% nervoso 12961 12 961 12.96 12% (12% cardiovascular regiões 90% 90 (90% 19 SUS Encontraramse Encontraram racional 20 10492 10 92 104.9 6696 6 96 66.9 (66 1770 1 70 17.7 (17 1296 12.9 (12 9 (90 2 1049 104. 669 66. (6 177 7 17. (1 129 12. (9 (

ABSTRACT. The present study aims to conduct a review on the potential use of insect meal as an alternative protein source in poultry feed, particularly to serve the rearing of free-range chickens. Insects are already part of the diet of birds in their natural habitat, and the availability of low-cost alternative foods with low environmental impact is essential for the development of the activity. The review comprehended studies that used meals consisting of silkworm (Bombyx mori) chrysalis, earthworm (Eisenia foetida), housefly (Musca domestica), black soldier fly (Hermetia illucens) and mealworm beetle (Tenebrio molitor) to replace plant-based protein sources. In general, insect meals have a high content of crude protein and ether extract, as well as an essential amino acid profile suitable for poultry feeding. The addition of insect meal in poultry feed normally shows good results as to growth performance and egg production, without causing a negative effect on carcass characteristics, meat sensory quality and egg quality, presenting itself as an alternative protein source with good prospects for replacing plant-based sources in poultry feed. ABSTRACT freerange free range chickens habitat lowcost cost activity Bombyx mori chrysalis Eisenia foetida, foetida , foetida) Musca domestica, domestica domestica) Hermetia illucens Tenebrio molitor plantbased plant based general extract feeding production characteristics

Abstract Introduction Although extracorporeal photopheresis (ECP) is a promising second-line therapy in the treatment of chronic graft-versus-host disease (cGVHD), its use is limited by its high cost. This study aims to describe the clinical evolution of patients who underwent ECP therapy for cGVHD and to perform an economic analysis of the therapy Methods This was a case series between 2016 and 2020 describing the clinical response to ECP and a micro-cost analysis of the therapy using time-driven activity-based costing. Results Six patients underwent ECP for corticosteroid-dependent cGVHD The cost per ECP session is 14,960.90 Brazilian reais (BRL), which primarily consists of the ECP kit with an activator (82.78%), followed by the hospital's physical structure (14.66%), human resources (2.48%) and exams/inputs (0.08%). The number of sessions performed ranged from 2 to 42. The total cost of the therapy per patient ranged from BRL 30,000 to 500,000. Conclusion The response of the patient with cGVHD to treatment with ECP was variable. These micro-costing results can be used to develop remuneration and cost control strategies in hematopoietic stem cell transplantation programs, as well as in further economic studies. (ECP secondline second line graftversushost graft versus host cGVHD, , (cGVHD) 201 202 microcost micro timedriven time driven activitybased activity based costing corticosteroiddependent corticosteroid dependent 1496090 14 960 90 14,960.9 BRL, (BRL) 82.78%, 8278 82.78% 82 78 (82.78%) hospitals hospital s 14.66%, 1466 14.66% 66 (14.66%) 2.48% 248 48 (2.48% examsinputs exams inputs 0.08%. 008 0.08% . 0 08 (0.08%) 42 30000 30 000 30,00 500000 500 500,000 variable microcosting programs studies (cGVHD 20 149609 1 96 9 14,960. (BRL 827 82.78 8 7 (82.78% 146 14.66 6 (14.66% 2.48 24 4 (2.48 00 0.08 (0.08% 3000 3 30,0 50000 50 500,00 14960 14,960 82.7 (82.78 14.6 (14.66 2.4 (2.4 0.0 (0.08 300 30, 5000 5 500,0 1496 14,96 82. (82.7 14. (14.6 2. (2. 0. (0.0 500, 149 14,9 (82. (14. (2 (0. 14, (82 (14 ( (0 (8 (1

Abstract Background Multiple sclerosis (MS) is an inflammatory demyelinating disease. Auditory evoked potential studies have demonstrated conduction and neural processing deficits in adults with MS, but little is known about the electrophysiological responses in children and adolescents. Objective to evaluate the central auditory pathway with brainstem auditory evoked potentials (BAEP) and long-latency auditory evoked potentials (LLAEP) in children and adolescents with MS. Methods The study comprised 17 individuals with MS, of both sexes, aged 9 to 18 years, and 17 healthy volunteers, matched for age and sex. All individuals had normal hearing and no middle ear impairments. They were assessed with click-BAEP and LLAEP through oddball paradigm and tone-burst stimuli. Results Abnormal responses were observed in 60% of electrophysiologic assessments of individuals with MS. In BAEP, 58.82% of MS patients had abnormal responses, with longer wave V latency and therefore longer III-V and I-V interpeak latencies than healthy volunteers. In LLAEP, 52.94% of MS patients had abnormal responses. Although statistical differences were found only in P2-N2 amplitude, MS patients had longer latencies and smaller amplitudes than healthy volunteers in all components. Conclusion Children and adolescents with MS had abnormal BAEP responses, with delayed neural conduction between the cochlear nucleus and the lateral lemniscus. Also, abnormal LLAEP results suggest a decrease in neural processing speed and auditory sensory discrimination response. (MS disease (BAEP longlatency long (LLAEP 1 sexes years sex impairments clickBAEP click toneburst tone burst stimuli 60 5882 58 82 58.82 IIIV III IV I 5294 52 94 52.94 P2N2 PN P2 N2 P N P2-N amplitude components lemniscus Also response 6 588 5 8 58.8 529 52.9 P2N 58. 52.

Resumo Antecedentes A esclerose múltipla (EM) é uma doença inflamatória desmielinizante. Estudos com potenciais evocados auditivos têm demonstrado déficits de condução e processamento neural em adultos com EM, mas pouco se sabe sobre as respostas electrofisiológicas em crianças e adolescentes. Objetivo avaliar a via auditiva central por meio dos potenciais evocados auditivos de tronco encefálico (PEATE) e dos potenciais evocados auditivos de longa latência (PEALL) em crianças e adolescentes com EM. Métodos Foram avaliados17 indivíduos com EM, de ambos os sexos, com idades entre 9 e 18 anos, e 17 voluntários saudáveis, pareados por sexo e idade. Todos os indivíduos tinham audição normal sem alterações de orelha média. Os indivíduos foram avaliados por meio do PEATE com estímulo clique e do PEALL com paradigma de oddball e estímulo tone-burst. Resultados Foram observadas alteração em 60% das avaliações dos indivíduos com EM. No PEATE, 58,82% dos pacientes com EM apresentaram alteração, com aumento da latência da onda V, e interpicos III-V e I-V aumentados em comparação aos voluntários saudáveis. No PEALL, 52,94% dos pacientes com EM apresentaram alteração. Embora diferenças estatísticas foram observadas apenas na amplitude P2-N2, os pacientes com EM apresentaram latências prolongadas e amplitudes menores em comparação aos voluntários saudáveis para todos os componentes. Conclusão Crianças e adolescentes com EM apresentaram alteração das respostas do PEATE, com atraso de condução neural entre o núcleo coclear e o lemnisco lateral. Além disso, os resultados alterados do PEALL sugeriram uma diminuição na velocidade de processamento neural e de discriminação sensorial da audição. (EM desmielinizante (PEATE (PEALL avaliados1 sexos 1 anos idade média toneburst. toneburst tone burst. burst tone-burst 60 5882 58 82 58,82 V IIIV III IV I 5294 52 94 52,94 P2N2, P2N2 PN P2 N2, N2 P N P2-N2 componentes lateral disso 6 588 5 8 58,8 529 52,9 P2N P2-N 58, 52,

BACKGROUND The parasite Giardia duodenalis infects a wide range of vertebrate hosts, including domestic and wild animals as well as humans. Giardia is genotyped into eight assemblages (A-H). Zoonotic assemblages A and B have already been identified in humans and wild and domestic animals (non-human primates and cats) from Brazilian Amazon and in the world. Due to its zoonotic/zooanthroponotic nature, surveillance initiatives and the definition of Giardia assemblages are important in order to characterise the epidemiological scenario and to implement further control measures. OBJECTIVES Determine assemblages of G. duodenalis in sloths from the Brazilian Amazon Region. METHODS Faecal parasitological examination of sloths from Amazonas State. Polymerase chain reaction (PCR) targeting the beta giardin (BG), and genes from multilocus sequence typing (MLST) scheme, amplicon sequencing and phylogenetic analysis. FINDINGS Here, we identified, by microscopy, Giardia in two northern sloths (Bradypus tridactylus). These two samples were submitted to molecular assays and it was revealed that both were infected by G. duodenalis assemblage A. Phylogenetic analysis showed that they belong to assemblage A within sequences from humans and wild and domestic animals. CONCLUSION Therefore, besides showing, by the first time, the current presence of this parasite in sloths, our findings reveals that this wild animal species would be part of the zoonotic/zooanthroponotic scenario of this parasite in the Brazilian Amazon. hosts AH. AH H . (A-H) nonhuman non human cats world zoonoticzooanthroponotic zoonotic zooanthroponotic nature measures G Region State PCR (PCR BG, BG , (BG) MLST (MLST scheme Here microscopy Bradypus tridactylus. tridactylus tridactylus) Therefore showing time (A-H (BG

RESUMO: Aeromonas sp. são bactérias presentes naturalmente no ecossistema aquático e apresentam potencial patogênico, sendo considerados patógenos emergentes em humanos e animais. Ainda, causam perdas consideráveis em piscicultura e, através da água, podem também contaminar inúmeros alimentos. Diante disso o objetivo do trabalho foi quantificar e analisar o perfil de resistência aos antimicrobianos de bactérias do gênero Aeromonas sp. em peixes. Um total de 72 amostras foram adquiridas, sendo duas variedades de peixes (peixe de couro Pseudoplatystoma fasciatum x Leiarius marmoratus e os peixes redondos Colossoma macropomum x Piaractus mesopotamicus e Colossoma macropomum x Piaractus brachypomus), dois tipos (fresco e congelado) e de três estabelecimentos comerciais (supermercado, feira e peixaria). As 55 culturas isoladas de Aeromonas foram avaliadas quanto ao perfil de resistência aos antimicrobianos pelo método de difusão em disco. Os valores encontrados na quantificação de Aeromonas sp. variaram de 4,22 a 6,00 Log UFC/g, e foram identificadas dez espécies diferentes, sendo A. eucrenophila, A. hydrophila, A. caviae, A. media, A. jandaei, A. veronii bv. sobria, A. trota, A. schubertii, A. veronii bv. veronii e A. shigelloides. Entre os 55 isolados, 64,45% apresentaram resistência a Ampicilina-sulbactam, 75% foram sensíveis à gentamicina e ciprofloxacina. Concluiu-se que 100% das amostras avaliadas estavam contaminadas por Aeromonas sp. podendo apresentar risco à saúde do consumidor visto que estas podem ser agentes etiológicos de Doenças Transmitidas por Alimentos (DTAs). O perfil de resistência aos antimicrobianos comprovou a resistência a ampicilina e apresentou multirresistência a diferentes classes de antimicrobianos, demonstrando um problema relacionado à opção de antimicrobiano para tratamento no caso do desenvolvimento de alguma doença.

ABSTRACT: The bacteria Aeromonassp. are naturally reported in aquatic ecosystems and possess pathogenic potential, being considered as emerging pathogens in humans and animals. They also cause considerable losses in fish farming and, through water, can contaminate numerous foods. This study quantified and analyzed the antimicrobial resistance profile of Aeromonassp. in fish. A total of 72 samples of two fish varieties (leather fish, Pseudoplatystomafasciatumx Leiariusmarmoratusand round fish, Colossomamacropomumx Piaractusmesopotamicusand Colossomamacropomumx Piaractusbrachypomus) were purchased from two types of sources (fresh and frozen) and three commercial establishments (supermarket, market, and fishmonger). The 55 isolated Aeromonascultures were evaluated for their antimicrobial resistance profile by the disc diffusion method. Upon quantification, the count of Aeromonassp. ranged from 4.22 to 6.00 Log CFU/g; ten different species, including A. eucrenophila, A. hydrophila, A. caviae, A. media, A. jandaei, A. veroniibv. sobria, A. trota, A. schubertii, A. veroniibv. veronii, and A. shigelloides, were identified. Among the 55 isolates, 64.45% showed resistance to Ampicillin-sulbactam, and 75% were sensitive to gentamicin and ciprofloxacin. It was concluded that 100% of the evaluated samples were contaminated by Aeromonassp., which may present a risk to consumer health since bacteria can be etiological agents of Foodborne Diseases. The antimicrobial resistance profile showed resistance to ampicillin and multi-resistance to different classes of antimicrobials, demonstrating problems with choosing an antimicrobial for treatment of any disease.

Abstract: The objective of this study was to evaluate the quality of milk and cheese and sensory analysis of cheese from goats that received a diet with different levels of sorghum silage in the diet. Twenty Anglonubian dairy goats were used, with an average age of 2 years, average live weight of 43.7 kg, distributed in a completely randomized design, with five replications. Moisture (P=0.001), total solids (P=0.001), fat (p=0.001), TDE (P=0.006) and protein (P=0.003) of goat cheese showed a significant difference due to the inclusion of silage from goat cheese. sorghum in the diet. The sensory attributes soft appearance (P<.0001), look (P<.0001), salty taste (P<.0001) and smooth texture (P=0.0430) showed a significant difference depending on the level of sorghum silage added to the diet. of goats. In the PC analysis, it is observed that three main components were needed to represent the variation of 64.84% of the data. PC 1 determined 29% of the data variation and was represented by the attributes with the highest averages: attractive aroma (5.87) and global acceptability (7.74). The inclusion of up to 45% of sorghum silage in the diet of goats increased milk and cheese fat, which favored high scores for the attributes of softness and global acceptance. Abstract used years 437 43 7 43. kg design replications P=0.001, P0001 P P=0.001 , 0 001 (P=0.001) p=0.001, p0001 p p=0.001 (p=0.001) P=0.006 P0006 006 (P=0.006 P=0.003 P0003 003 (P=0.003 P<.0001, P<.0001 0001 (P<.0001 P=0.0430 P00430 0430 (P=0.0430 6484 64 84 64.84 29 averages 5.87 587 5 87 (5.87 7.74. 774 7.74 . 74 (7.74) 45 acceptance 4 P000 P=0.00 00 (P=0.001 p000 p=0.00 (p=0.001 (P=0.00 P<.000 000 (P<.000 P=0.043 P0043 043 (P=0.043 648 6 8 64.8 5.8 58 (5.8 77 7.7 (7.74 P00 P=0.0 p00 p=0.0 (p=0.00 (P=0.0 P<.00 (P<.00 P=0.04 P004 04 (P=0.04 64. 5. (5. 7. (7.7 P0 P=0. p0 p=0. (p=0.0 (P=0. P<.0 (P<.0 (5 (7. P=0 p=0 (p=0. (P=0 P<. (P<. ( (7 P= p= (p=0 (P= P< (P< (p= (P (p

Abstract The study analyzed the consumption of non-standard medication in the health of indigenous peoples, emphasizing the rationality of pharmacotherapy, by conducting a cross-sectional study of secondary data from 2018 and 2019 in the Minas Gerais/Espírito Santo Special Sanitary Indigenous Health Districts. These medicines were classified by Anatomical Therapeutic Chemical Classification. Non-parametric tests were applied to compare the origin of prescription and the form of acquisition, assessing access to medication. Rationality was verified through the consumption profile and the therapeutic option in the list of standardized medicines. A total of 104,928 pharmaceutical presentations were consumed, 66,967 (66%) for the alimentary tract and metabolism, 17,705 (17%) for the nervous system, and 12,961 (12%) for the cardiovascular system. With respect to medicines consumed per region, 171 (90%) out of 190 had a therapeutic option. Prescriptions were more from the SUS. Differences were found in the way the medicines were acquired. The study pointed to significant consumption of non-standard medicines, and there may be failings in therapeutic rationality. In indigenous health, ethnocultural and social issues are challenges to access to medicines with rational use.

Resumo O estudo analisou o consumo de medicamentos não padronizados na saúde indígena, enfatizando a racionalidade da farmacoterapia, por meio de um estudo transversal dos dados secundários, de 2018 e 2019, no Distrito Especial Sanitário de Saúde Indígena Minas Gerais/Espírito Santo. Esses medicamentos foram classificados pela Anatomical Therapeutic Chemical Classification. Para a comparação da origem de prescrição e da forma de aquisição, empregaram-se testes não paramétricos, avaliando o acesso a medicamentos. Verificou-se a racionalidade por meio do perfil de consumo e da opção terapêutica na lista de medicamentos padronizados. Foram consumidas 104.928 apresentações farmacêuticas, 66.967 (66%) eram para o trato alimentar e o metabolismo; 17.705 (17%) para o sistema nervoso; 12.961 (12%) para o sistema cardiovascular. Quanto aos medicamentos mais consumidos por regiões, 171 (90%) dos 190 apresentavam opção terapêutica. As prescrições foram mais provenientes do SUS. Encontraram-se diferenças na forma de aquisição dos medicamentos. O estudo apontou importante consumo de medicamentos não padronizados, podendo existir falhas na racionalidade terapêutica. Na saúde indígena, questões etnoculturais e sociais constituem desafios para o acesso aos medicamentos com uso racional.

SUMMARY OBJECTIVE: The polycystic ovary syndrome is the most common endocrine disorder, characterized by the dysregulation of ovarian angiogenesis. This alteration can be related to changes in the activities of the vascular endothelial growth factor (VEGF) gene. Single-nucleotide polymorphisms have been observed in the promoter, intronic, and untranslated regions of the VEGF gene, and several studies have suggested that these polymorphisms may be associated with the risk of polycystic ovary syndrome. This study aimed to investigate the association between rs2010963 and rs833061 polymorphisms and haplotypes of VEGF in the etiology of polycystic ovary syndrome. METHODS: A total of 210 women, 102 diagnosed with polycystic ovary syndrome and 108 controls, participated in this study. The genotyping of the samples was performed by PCR-RFLP and real-time PCR for rs2010963 and rs833061 polymorphisms, respectively. The statistical analyses were performed by the chi-square test and logistic regression model. RESULTS: The clinical characteristics of the patients showed that 75.8% of the patients did not become pregnant, 36.3% had a family history of polycystic ovary syndrome, 58.6% were obese, and about 60% had clinical characteristics of hyperandrogenism. There were no associations between the distribution of rs2010963 (OR 1.24; 95%CI 0.60–2.57; p=0.56) and rs833061 (OR 0.78; 95%CI 0.32–1.92; p=0.59) in patients and controls. CONCLUSIONS: The patients with polycystic ovary syndrome have similar rates of VEGF polymorphisms rs2010963 and rs833061 on the general population.

Abstract We evaluated the accuracy of radiomorphometric indices (RI) and fractal dimension (FD) for screening bone mineral density (BMD) in postmenopausal patients who had breast cancer and were using aromatase inhibitors (AI). The sample consisted of 40 participants. Digital panoramic radiography (DPR) and cone beam computed tomography (CBCT) were evaluated along with dual-energy X-ray absorptiometry (DXA), which is the gold standard for detecting low BMD. According to the T-scores of DXA, the subjects were assigned into two groups: with normal BMD and with low BMD (osteopenia and osteoporosis). The area under the curve (AUC), sensitivity, and specificity with their respective confidence intervals were determined for DPR and CBCT. For DPR indices, AUC ranged from 52.6 to 75.8%. The mandibular cortical width (MCW) had the highest AUC. For FD, the total trabecular index had the highest sensitivity, while the index anterior to the mental foramen (MF) had the highest specificity. In CBCT, the AUC ranged from 51.8 to 62.0%. The indices with the highest AUC were the molar (M) and anterior (A). The symphysis (S) index had the highest sensitivity and the posterior (P) index had the highest specificity. Sensitivity and specificity were adequate for the computed tomography index (Inferior; CTI [I]). Therefore, MCW, FD of the mandible angle, and total trabecular ROI in DPR and the CTI (I), M, P, and A indices in CBCT proved to be promising tools in distinguishing individuals with low BMD. Cutoff point for these indices could be a useful tool to investigate low BMD in postmenopausal women taking AI.