Abstract The honey bee Apis mellifera is a plant-pollinator that produces commercial products. It has female castes with workers performing tasks in the colonies and a queen, it has the longest lifespan, in charge of reproduction. This bee undergoes a population decline worldwide. Therefore, it is important to understand how aging affects the digestive tract of this insect. In the midgut, regenerative cells are organized in nests replacing the dead cells. This study verified the hypothesis that the number of regenerative cells decreases as A. mellifera queen ages. The midgut was evaluated in queens at the age of four days and eight, 11 and 25 months. The midgut of 11- and 25-month-old queens presented signs of epithelial disorganization in comparison with younger queens. The number of regenerative cell nests in the midgut decreases according to the age of the queen, but the number of cells per nest is similar. The decreased number of regenerative cell nests reveals a potential loss in the amount of these cells available for the renewal of the midgut epithelium. The absence of variations in the number of regenerative cells per nest according to the queen age indicates that these cells do not undergo proliferation before the differentiation.

RESUMO Os cupins sem soldados (Blattodea: Isoptera: Apicotermitinae) são um grupo pequeno e taxonomicamente pouco conhecido, principalmente na região neotropical. Neste artigo descrevemos um novo gênero monotípico Caputitermes gen. nov., que possui a junção mesênetero-P1 reta e ausência de segmento misto. A espécie Caputitermes dimorphus sp. nov., se destaca por ter dois tipos de operários. Blattodea (Blattodea Isoptera Apicotermitinae conhecido neotropical gen nov nov. mesêneteroP1 mesêneteroP mesênetero P1 P mesênetero-P misto sp operários

ABSTRACT Soldierless termites (Blattodea: Isoptera: Apicotermitinae) are a small and taxonomically poorly known group, especially in the Neotropical region. In this paper we describe a new monotypic genus Caputitermes gen. nov. with a straight mesenteron-P1 junction, and absence of mixed segment. The species Caputitermes dimorphus sp. nov., which stands out for having two types of workers. Blattodea (Blattodea Isoptera Apicotermitinae group region gen nov mesenteronP1 mesenteronP mesenteron P1 P mesenteron-P junction segment sp workers

Abstract The world is currently facing a severe economic crisis and the rational consumption and reuse of products, including waste are necessary. Agriculture wastes have importance containing beneficial and economic value matters, which unutilized may lead to environmental pollution. Date seed is one of these viable wastes with high nutritional value. Mass rearing of peach fruit flies is important to produce parasitoids for use in biological control programs or sterile insect techniques. In this study powder of date seeds were evaluated as an alternative diet for mass rearing of peach fruit fly Bactrocera zonata. The nutrient composition of the tested diet that contained date seed powder was rich in crude proteins (13.1%), crude carbohydrates (40.41), crude fats (4.6), crude fibers (8.4), and ashes (0.29). The results indicated that the date seed powder produced insects with quality parameters equivalent to FAO, IAEA/USDA standards, without differences with those reared on yeast. The histology of the reproductive system of B. zonata reveals that the female ovariole is of meristic-polytrophic type with successive stages of egg chambers. The male testis has a single long follicle with successive stages of spermatogenesis. Thus, date seed powder is a potential alternative diet for use in the mass rearing of the peach fruit fly.

Abstract Production and sale of botanical insecticides depend on knowing the potential opportunities for these products. Essential oils from plants secondary metabolism can control pests, especially in agricultural systems where synthetic insecticides are limited, as in organic agriculture. The objective of this study was to evaluate the toxicity of essential oils to Diaphania hyalinata (Lepidoptera: Crambidae) and to show regions with the potential to use Cinnamomum zeylanicum, Citrus sinensis, and Syzygium aromaticum in the formulation and commercialization of insecticides to control this insect. The C. zeylanicum oil was more toxic to larvae and pupae and the S. aromaticum to eggs of D. hyalinata. Essential oils are an alternative for the management of D. hyalinata. The production of pesticides from essential oils of C. zeylanicum, C. sinensis, and S. aromaticum to control D. hyalinata has high potential in America. Also, Asia, Africa, Europe, the Middle East, and Asia can extract these plants to formulate insecticide molecules for the America countries. products pests limited agriculture Lepidoptera (Lepidoptera Crambidae sinensis insect C S D Also Africa Europe East countries

Abstract Ants are social insects with about 85 exocrine glands with different morphologies. The metapleural gland is exclusive to ants and its compounds have antimicrobial action, indicating a function in the defense against pathogens. Within ants, Paraponera clavata is the only living species representative of Paraponerinae. This study describes the anatomy and histology of the metapleural gland in workers of P. clavata. The metapleural gland is formed by secretory units that release secretion in a reservoir through collecting canaliculi. Secretory units are bicellular with a well-developed globular secretory cell, with 145 µm in diameter, and a duct cell forming the collecting canaliculi characterizing a class III gland. The metapleural gland is pointed out as a synapomorphy of ants. Paraponerinae constitutes one of the six subfamilies of the basal and paraphyletic Poneroide clade and knowledge of the metapleural gland morphology contribute to the understanding of evolutionary history of the basal ants’ groups.

Resumo A diversidade de fungos, incluindo espécies entomopatogênicas, é alta nos solos. Esses fungos são utilizados no manejo de pragas com facilidade de produção, multiplicação, aplicação e dispersão no campo. O objetivo foi avaliar a diversidade de fungos entomopatogênicos em solos de culturas de eucalipto e soja e áreas de mata nativa. Fungos entomopatogênicos foram isolados pelo “Bait Method” com larvas de Tenebrio molitor (Linnaeus, 1758) (Coleoptera: Tenebrionidae) de 10 amostras de solo por área, coletadas a 10 cm de profundidade em zig-zag. Os fungos isolados foram cultivados em três placas de Petri em meio BDA e seus micélios separados após sete dias de incubação em câmara tipo BOD. Fungos dos gêneros Aspergillus, Beauveria, Cordyceps, Fusarium, Metarhizium, Penicillium e Purpureocillium foram identificados. O “Bait Method” com larvas de T. molitor é eficiente para isolar fungos entomopatogênicos com maior diversidade em solos de área de mata nativa que naqueles com culturas de eucalipto e soja.

Abstract Soils present high fungal diversity, including entomopathogenic species. These fungi are used in pest control, providing easy production, multiplication, application, and dispersion in the field. The objective of the present study was to evaluate entomopathogenic fungal diversity in soils from eucalyptus and soybean crops and natural forest areas. These fungi were isolated using the “Bait Method” with Tenebrio molitor (Linnaeus, 1758) (Coleoptera: Tenebrionidae) larvae from 10 soil samples per area, collected at 10 cm deep in a zig-zag pattern. The isolated entomopathogenic fungi were cultivated in Petri dishes using PDA medium and their mycelia separated after seven days of incubation in a BOD-type chamber. Species of Aspergillus, Beauveria, Cordyceps, Fusarium, Metarhizium, Penicillium and Purpureocillium were identified. The “Bait Method” with T. molitor larvae is efficient to isolate entomopathogenic fungi with higher diversity from soils of the natural forest than the cultivated area.

ABSTRACT An important concern with the use of genetically modified (GM) plants expressing Bacillus thuringiensis (Bt) insecticidal toxins is the deleterious effect on non–target organisms. The predatory stink bug Podisus nigrispinus (Dallas) (Hemiptera: Pentatomidae) is used in biological control programs and may be exposed to Bt toxins. This study evaluated the indirect effects of different Cry proteins on P. nigrispinus with the prey Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae), fed on simple or pyramided Bt maize genotypes. The experiment was carried out in a completely randomized design with three treatments: i) Isohybrid (not Bt), ii) Herculex® (transgenic maize encoding Cry1F protein) and iii) PowerCore® (pyramidal transgenic maize encoding the Cry1F, Cry1A.105, and Cry2Ab2 proteins), which were used to feed S. frugiperda for 48 h. The caterpillars were used as prey by P. nigrispinus females. We evaluated the presence of Cry proteins, consumed prey biomass (predation), oviposition period, number of postures, number of eggs, number of eggs per posture, number of nymphs, egg viability, embryonic period, female longevity and development, and survival rates of immature. The results show that different Cry proteins move through the food chain of P. nigrispinus and provide evidence that the ingestion of three different proteins does not lead to unexpected synergistic effects. However, Cry toxins promoted histopathological changes in midgut cells of P. nigrispinus.

Resumo Como é que os pais percecionam o papel do Conselho Geral das Escolas (CGE), que valor lhe atribuem enquanto órgão de direção estratégica, como se inscrevem na ação que aí desenvolvem, são as questões às quais quisemos responder. Para tal, através da Confederação Nacional das Associações de Pais (CONFAP), enviamos um inquérito por questionário destinado aos pais que exerciam funções nesse órgão, constituído por 28 itens, posteriormente categorizados em seis dimensões: participação, deliberação, focalização, divulgação, relevância e relação entre pais e diretor. O questionário foi administrado on-line, entre 18 de dezembro de 2017 e 26 de janeiro de 2018 e recolhidas 213 respostas válidas. Os resultados foram tratados com recurso a estatística descritiva e inferencial, apurando-se a frequência, a média, desvio-padrão e análise de relações entre variáveis dependentes e independentes. Como principais conclusões retêm-se as que se relacionam com uma expressiva dispersão de resultados evidenciando uma visão heterogénea sobre as funções e relevância estratégica do CGE, o reconhecimento do condicionamento dos poderes externos (sediados no Ministério da Educação) e internos adstritos ao poder cognoscitivo dos professores e do diretor, uma sobrevalorização simbólica do órgão que não corresponderá à ação concreta, e uma visão restrita de comunidade educativa onde não cabem a autarquia e os alunos.

Resumen Cómo ven los padres el papel del Consejo General de las Escuelas, qué valor le atribuyen como órgano de "dirección estratégica", cómo se inscriben y se manifiestan en la acción que allí desarrollan son las cuestiones clave a las que hemos querido responder. Para ello, con la colaboración de la Confederación Nacional de las Asociaciones de Padres (CONFAP), enviamos una encuesta por cuestionario dirigida a los padres que ejercían funciones en ese órgano. El cuestionario constaba de 28 ítems, posteriormente categorizados en 6 dimensiones: participación, deliberación, focalización, divulgación, relevancia y relación entre padres y director. El cuestionario se administró on-line y fue respondido entre el 18 de diciembre y el 26 de enero de 2018, recogiéndose 213 respuestas válidas. Los resultados se trataron mediante una estadística descriptiva, calculando la frecuencia, la media y la desviación estándar y análisis de relaciones entre variables. Las principales conclusiones se relacionan con una significativa dispersión de resultados, evidenciando una visión heterogénea de las funciones y la relevancia estratégica del consejo general, el reconocimiento del condicionamiento de los poderes externos (localizados en el Ministerio de Educación) e internos, al poder cognitivo de los profesores y del director, una sobrevalorización simbólica del órgano que no se corresponde con la acción concreta, y una visión restringida de la comunidad educativa donde no caben la autarquía municipal y los alumnos.

Abstract What do parents think about the role of the General Council of schools, what value do they give it as a “strategic management” organ, how do they get involved and consider themselves in the role they play there, these are the key issues to which we’d like to answer. To do so, we sent a survey by questionnaire to parents who had a place in this organ, which was composed by 28 items categorized later into six dimensions: participation, deliberation, focus, dissemination, importance and relationship between parents and the school director. The questionnaire was sent to be answered through a link between December 18 and January 26, 2018 and 213 valid answers were collected. The questionnaire results were processed by using a descriptive statistics method to get the frequency, mean and standard deviation and analysis of relations between variables. As main conclusions, we emphasize those related to a significant dispersal of results which demonstrate an heterogeneous view about the roles of its members and the strategic importance of the General Council, the recognition of the conditioning of the external forces (placed in the Education Ministry) and the internal forces related to the cognitive power of the teachers and the director, a symbolic overvaluation of this organ which will not correspond to its concrete action and a limited view of the educational community where there’s no place either for the local council or the students.

ABSTRACT The objective of this study was to evaluate cotton cultivars based on the compensatory increase of cotton squares in response to the simulation of the boll weevil damage. The first experiment aimed to evaluate cotton cultivars with greater compensatory increase in squares removed artificially from the plant. The experimental design was in randomized blocks, in a 4 × 2 factorial scheme with 100% removal of cotton squares at 50, 70 and 90 days after the emergence of the FM975WS, TMG81WS, IMA6501B2RF and BRS432B2RF cotton cultivars and passive production (without removal) and active (with removal) of cotton squares, with four replications. In the second experiment, boll weevil damage in the TMG80WS and BRS432B2RF, with greater and lesser compensatory increase of cotton squares, after their removal at 50 days of age, was evaluated. The experimental design was in randomized blocks, in a 2 × 2 factorial scheme, with the former cultivars sprayed with carbamate or not (control). The compensatory increase of cotton squares, mainly after their artificial removal at 50 and 70 days of age, of the TMG81WS, was greater. The cotton square compensatory increase after being artificially removed in the cotton cultivars was higher during the period of exponential production of these reproductive structures and can be used to select cultivars tolerant to boll weevil damage.

ABSTRACT The Hermanella complex is a well-established monophyletic group of Hagenulinae mayflies, but the phylogenetic relationships among its members are still being discussed. In order to fill some gaps in the taxonomy of this group we describe, for the first time, the eggs of eight species of this complex (Hermanella amere, Hermanella mazama, Hydromastodon sallesi, Hydrosmilodon gilliesae, Hydrosmilodon plagatus, Leentvaaria palpalis, Paramaka incognita, Paramaka sp.) using scanning electron microscopy, including some important members whose generic allocation have been questioned. The egg morphology in these eight species of Hermanella complex and their similarities with other species of the complex are discussed.

ABSTRACT Members of the Hydrophilidae, one of the largest families of aquatic insects, are potential models for the biomonitoring of freshwater habitats and global climate change. In this study, we describe the morphology of the male reproductive tract in the water scavenger beetle Tropisternus collaris. The reproductive tract in sexually mature males comprised a pair of testes, each with at least 30 follicles, vasa efferentia, vasa deferentia, seminal vesicles, two pairs of accessory glands (a bean-shaped pair and a tubular pair with a forked end), and an ejaculatory duct. Characters such as the number of testicular follicles and accessory glands, as well as their shape, origin, and type of secretion, differ between Coleoptera taxa and have potential to help elucidate reproductive strategies and the evolutionary history of the group.

RESUMO Basidiósporos do fungo ectomicorrízico Pisolithus microcarpus apresentam parede celular impermeável, característica possivelmente relacionada às baixas porcentagens de germinação desses propágulos, o que dificulta a obtenção de monocários e a utilização desses esporos em inoculantes. O objetivo deste estudo foi avaliar o efeito de diferentes concentrações de água sanitária comercial na permeabilização dos basidiósporos de P. microcarpus e analisar as alterações causadas na ultraestrutura da parede celular, na viabilidade e na capacidade germinativa desses propágulos. Basidiósporos fúngicos foram coletados em plantações de eucalipto e permeabilizados utilizando-se diferentes concentrações de água sanitária e tempos de exposição. Os basidiósporos foram então analisados por microscopia eletrônica de varredura e de transmissão. A porcentagem de basidiósporos permeabilizados variou com a marca comercial, concentração de água sanitária e o tempo de exposição. Basidiósporos de diferentes basidiocarpos diferiram na suscetibilidade ao tratamento de permeabilização com água sanitária. Alterações da ultraestrutura dos basidiósporos permeabilizados foram observadas nas concentrações de 15 e 50 % de água sanitária pelo tempo de exposição de 40 s, com mudanças de superfície e perda das espículas da camada mais externa da parede. Após a permeabilização com água sanitária a 5 % por 40 s, 80 % dos esporos permeabilizados encontravam-se viáveis, resultando na produção de colônias fúngicas após 15 dias de incubação desses propágulos na presença de Corymbia citriodora. No entanto, a porcentagem de germinação obtida: 0,001%, foi semelhante à de basidiósporos não-permeabilizados, indicando que outros fatores, além da permeabilidade da parede celular, são determinantes para o processo de germinação.

ABSTRACT Basidiospores of the ectomycorrhizal fungus Pisolithus microcarpus have an impermeable cell wall, a characteristic that is possibly related to the low germination percentages of these propagules, which makes it difficult to obtain monokaryons and use these spores in inoculants. The objective of this study was to evaluate the effect of different concentrations of commercial bleach on the permeabilization of P. microcarpus basidiospores and to analyze the alterations caused in the cell wall ultrastructure and the viability and germination capacity of these propagules. Fungal basidiospores were collected in eucalyptus plantations and permeabilized using different bleach concentrations and exposure times. The basidiospores were then analyzed by scanning and transmission electron microscopy. The percentage of permeabilized basidiospores varied with the commercial brand, bleach concentration, and exposure time. Basidiospores of different basidiocarps differed in susceptibility to permeabilization treatment with bleach. Changes in the ultrastructure of permeabilized basidiospores were observed at bleach concentrations of 15 and 50 % for an exposure time of 40 s, with surface changes and loss of the spicules of the outermost layer of the wall. After permeabilization with 5 % bleach for 40 s, 80 % of the permeabilized spores were viable, resulting in the production of fungal colonies after 15 days of incubation of these propagules in the presence of Corymbia citriodora. However, the germination percentage obtained, 0.001 %, was similar to that of non-permeabilized basidiospores, indicating that other factors, besides cell wall permeability, are determinant for the germination process.

Resumo Este estudo apresenta o tratamento taxonômico de Anacardium para o estado do Pará (Brasil), baseado na análise de espécimes provenientes de herbários (CEN, ESA, IAN, INPA, MBM, MFS, MG, RB, UB e UEC), materiais coletados em campo e imagens de espécimes disponíveis em bancos de dados virtuais. As identificações das espécies foram confirmadas por consulta aos protólogos e tipos ou imagens dos tipos. A análise morfoanatômica das folhas de Anacardium foi realizada a fim de dar suporte às caracterizações taxonômicas. São apresentadas descrições, pranchas, um mapa de distribuição e uma chave de identificação para as espécies de Anacardium ocorrentes no Pará (A. amapaense, A. giganteum, A. humile, A. microsepalum, A. occidentale e A. spruceanum).

Abstract This study presents a taxonomic treatment of Anacardium for the state of Pará (Brazil), based on the analysis of specimens from herbaria (CEN, ESA, IAN, INPA, MBM, MFS, MG, RB, UB, and UEC), material collected in the field, and images of specimens from virtual databases. Species identifications were confirmed by consultation of protologues and types or images of types. A morphoanatomical analysis of the leaves of Anacardium was conducted to support taxonomic characterizations. Descriptions, plates, a distribution map, and an identification key for the species of Anacardium occurring in Pará (A. amapaense, A. giganteum, A. humile, A. microsepalum, A. occidentale, and A. spruceanum) are provided.

ABSTRACT Wasps are a diverse group of insects that possess a sting apparatus associated with a venom gland, which is used for predation and colony defense. The biochemistry of Hymenoptera venom has been evaluated in relation to allergy and immunology, and proteomics has been shown to be a powerful tool for the identification of compounds with pharmacological potential. Data on wasps venom the of genus Apoica are scarce, so the objective of the present work was to identify the venom proteins of the eusocial wasp Apoica pallens, as a first step towards further investigation of applied uses of the venom and its protein constituents. The venom proteins were separated by two-dimensional gel electrophoresis, followed by MALDI-TOF/TOF mass spectrometry. A total of 259 spots were detected, with molecular weights from 4.9 to 141 kDa. Thirty of these proteins were identified and classified into eight functional categories: allergen, enzyme, metabolism, structural, environmental response, proteoglycan, active in DNA and RNA, and unknown function. Due to the few available proteomic data for wasp venom, many proteins could not be identified, which makes studies with proteomic analysis of Hymenoptera venom even more important.

RESUMO Introdução: A prova de 100 metros rasos (100 m) apresenta um apelo especial. Treinadores e pesquisadores buscam compreender os determinantes do desempenho nessa tarefa. Embora informações tenham sido desenvolvidas ao longo dos anos, elas não são completamente aplicadas pelos treinadores que, em geral, avaliam o sucesso dos métodos de treinamento empregados por meio de testes de campo objetivos, como o desempenho no teste de 60 m rasos. Objetivo: Investigar o desempenho de 100 m com base no desempenho de 60 m. Métodos: Duzentos e quarenta e seis homens e 153 mulheres, divididos em dois subgrupos, foram avaliados quanto à estimativa (Fvalidação; n=123 e Mvalidação; n=204) e validação de modelos preditivos (Fvalidação cruzada; n=30 e Mvalidação cruzada; n=42) para o desempenho de 100 m (tempo para percorrer 100 m). O tempo parcial foi medido baseado na distância de 100 m previamente sinalizada a cada 10 metros, a partir da linha de partida em ambos os lados da pista. Os modelos preditivos foram estimados no intervalo nos 60 metros a partir do intervalo de tempo de 10-10m. Resultados: A magnitude da correlação encontrada foi muito alta. Altos coeficientes de determinação e diferenças sem significância estatística (p<,001) foram encontrados entre os critérios e os valores previstos. As equações preditivas apresentaram valores de erro constantes inferiores a 0,001s; erro total absoluto de 0,12s; 0,10s para Mvalidação e Fvalidação, respectivamente, e 1,13% e 0,85% de erro relativo total para Mvalidação e Fvalidação, respectivamente. A análise de Bland-Altman mostrou um aumento no nível de concordância entre os critérios e os valores previstos para Fvalidação e Mvalidação. Respostas semelhantes foram encontradas quando aplicados os modelos propostos para Fvalidação cruzada e Mvalidação cruzada. Conclusão: Os modelos de estimativa foram capazes de prever com precisão o desempenho de 100 m a partir do desempenho no teste de 60 m. Nível de evidência II; Estudos diagnósticos - Investigação de um exame para diagnóstico.

ABSTRACT Introduction: The 100-meter dash (100 m) event holds particular appeal. Coaches and researchers seek to understand the determinants of performance in this task. Although information has been produced over the years, it is not fully applied by coaches who generally assess the success of employed training methods through objective field tests, such as 60 m dash test performance. Objective: Investigate 100 m performance based on 60 m performance. Methods: Two hundred and forty six men and 153 women divided into two subgroups were evaluated for estimation (Fvalidation; n=123 and Mvalidation; n=204) and validation of predictive models (Fcross-validation; n=30 and Mcross-validation; n=42) for 100 m dash performance (time take to cover 100 m). Partial time was measured based on the 100 m distance marked previously every 10 meters from the starting line on both sides of the track. The predictive models were based on the interval in the 60 meters with a time interval of 10-10 m. Results: Magnitude of correlation was very high. High coefficients of determination and differences of no statistical significance (p <.001) were found between the criteria and predicted values. The predictive equations presented constant error values below 0.001s; total absolute error of 0.12s; 0.10s for Mvalidation and Fvalidation, respectively, and 1.13% and 0.85% of total relative error for Mvalidation and Fvalidation, respectively. Bland-Altman analysis showed an increase in the level of concordance between the criteria and predicted values of Fvalidation and Mvalidation. Similar responses were found when the proposed models were applied to Fcross-validation and Mcross-validation. Conclusion: The estimation models were able to accurately predict 100 m performance based on 60 m performance. Level of evidence: II; Diagnostic studies - Investigating a diagnostic test.

RESUMEN Introducción: La prueba de 100 metros rasos (100 m) presenta un apelo especial. Entrenadores e investigadores buscan comprender los determinantes del desempeño en esa tarea. Aunque hayan sido desarrolladas informaciones a lo largo de los años, ellas no son completamente aplicadas por los entrenadores que, en general, evalúan el éxito de los métodos de entrenamiento empleados por medio de tests de campo objetivos, como el desempeño en el test de 60 m rasos. Objetivo: Investigar el desempeño de 100 m con base en el desempeño de 60 m. Métodos: Doscientos cuarenta y seis hombres y 153 mujeres, divididos en dos subgrupos, fueron evaluados cuanto a la estimativa (Fvalidación; n=123 y Mvalidación; n=204) y validación de modelos predictivos (Fvalidación cruzada; n=30 y Mvalidación cruzada; n=42) para el desempeño de 100 m (tiempo para recorrer 100 m). El tiempo parcial fue medido con base en la distancia de 100 m previamente señalizada a cada 10 metros, a partir de la línea de largada en ambos lados de la pista. Los modelos predictivos fueron estimados en el intervalo en los 60 metros a partir del intervalo de tiempo de 10-10m. Resultados: La magnitud de la correlación encontrada fue muy alta. Altos coeficientes de determinación y diferencias sin significancia estadística (p<,001) fueron encontradas entre los criterios y los valores previstos. Las ecuaciones predictivas presentaron valores de error constantes inferiores a 0,001s; error total absoluto de 0,12s; 0,10s para Mvalidación y Fvalidación, respectivamente, y 1,13% y 0,85% de erro relativo total para Mvalidación y Fvalidación, respectivamente. El análisis de Bland-Altman mostró un aumento en el nivel de concordancia entre los criterios y los valores previstos para Fvalidación y Mvalidación. Respuestas semejantes fueron encontradas cuando aplicados los modelos propuestos para Fvalidación cruzada y Mvalidación cruzada. Conclusión: Los modelos de estimativa fueron capaces de prever con precisión el desempeño de 100 m a partir del desempeño en el test de 60 m. Nivel de evidencia II; Estudios diagnósticos - Investigación de un examen para diagnóstico.