Abstract The control of weeds in agriculture is mainly conducted with the use of synthetic herbicides. However, environmental and human health concerns and increased resistance of weeds to existing herbicides have increased the pressure on researchers to find new active ingredients for weed control which present low toxicity to non-target organisms, are environmentally safe, and can be applied at low concentrations. It is herein described the synthesis of glycerol-fluorinated triazole derivatives and evaluation of their phytotoxic and cytogenotoxic activities. Starting from glycerol, ten fluorinated triazole derivatives were prepared in four steps. The assessment of them on Lactuca sativa revealed that they present effects on phytotoxic and cytogenotoxic parameters with different degrees of efficiency. The compounds 4a, 4b, 4d, 4e, 4i, and 4j have pre-emergent inhibition behavior, while all the investigated compounds showed post emergent effect. Mechanism of action as clastogenic, aneugenic, and epigenetic were observed in the lettuce root meristematic cells, with alterations as stick chromosome, bridge, delay, c-metaphase, and loss. It is believed that glycerol-fluorinated triazole derivatives possess a scaffold that can be explored towards the development of new chemicals for the control of weed species. However nontarget non target organisms safe concentrations glycerolfluorinated glycerol activities steps efficiency 4a 4b b 4d d 4e e 4i i j preemergent pre behavior effect clastogenic aneugenic cells chromosome bridge delay cmetaphase, cmetaphase c metaphase, metaphase c-metaphase loss species

Objective: To assess differences in blood inflammatory cytokines between people with alcohol use disorder (AUD) and healthy controls (HC). Methods: Searches were performed from inception through April 14, 2021. Meta-analyses with random-effects models were used to calculate the standardized mean difference ([SMD], 95%CI), and potential sources of heterogeneity were explored trough meta-regressions and subgroup analysis. Results: The meta-analysis included 23 studies on the following 14 cytokines: tumor necrosis factor (TNF)-α, IL-1, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-13, IL15, interferon (IFN)-γ and sCD14. There were significantly higher concentrations of IL-6 (n=462 AUD and 408 HC; SMD = 0.523; 95%CI 0.136-0.909; p = 0.008) in AUD than HC. No significant differences were found in the other 13 cytokines. Conclusion: We found that IL-6 levels were significantly higher in individuals with AUD than HC and that other cytokines were not altered. This can be explained by the small number of studies, their methodological heterogeneity, and confounding factors (active use, abstinence, quantity, and physical or psychiatric illnesses, for example). Despite a great deal of evidence about alcohol and inflammatory diseases, studies assessing the role of neuroimmune signaling in the development and severity of AUD are still lacking.

RESUMO Objetivou-se avaliar o consumo, a digestibilidade e o desempenho de ovinos, arraçoados com dietas isoproteicas, contendo 75% de resíduos da agroindústria frutífera (abacaxi, acerola, maracujá e cupuaçu), uma dieta com feno de tifton e 25% de concentrado, em delineamento inteiramente ao acaso. A participação do resíduo de maracujá na dieta proporcionou os maiores níveis de consumo de matéria seca (1170,6g d-1) em relação aos resíduos de abacaxi (693,8g d-1), acerola (644,2g d-1), cupuaçu (452,9g d-1) e feno de tifton (962,7g d-1), o que se relaciona aos seus níveis de digestibilidade (77,8; 63,1; 59,1; 61,7 e 71,8%, respectivamente). Tal resultado gerou diferenças significativas, destacando-se o resíduo de maracujá quanto à conversão alimentar (8,3), e o ganho médio diário de peso (141,5g d-1). Apesar de ter tido a mesma conversão observada para o feno de tifton (13,2), apresentou maior ganho médio diário (75,6g d-1). Os demais resíduos apresentaram efeitos sobrepostos, contudo o resíduo de acerola ficou com a pior conversão (51,4) e o menor ganho médio diário (15,6g d-1), sendo esse sem diferença em relação ao cupuaçu (23,0g d-1). Os resíduos podem ser utilizados como alternativa para a alimentação de ovinos.

ABSTRAT The objective was to evaluate the consumption, digestibility and performance of the sheep with iso protein diets, with 75% of residues from fruit agro-industries (pineapple, acerola, passion fruit, and cupuassu) and a diet with tifton hay, and 25% of concentrate, in a totally randomized design. The participation of passion fruit residue on the diet provided the highest consumption levels of dry matter (1170.6g d -1 ) compared with the residues of pineapple (693.8g d -1 ), acerola (644.2g d -1 ), cupuassu (452.9g d -1 ) and tifton hay (962.7g d -1 ), which is related to their levels of digestibility (77.85, 63.14, 59.07, 61.68 and 71.83%, respectively). Such a result generated significant differences with emphasis on passion fruit residue regarding food inversion (8.30) and average daily weight gain (141.50g d -1 ), in spite of having the same conversion detected for tifton hay (13.2), it showed a higher average daily gain (75.6g d -1 ). The other residues showed overlapping effects, however, acerola residue showed the worst conversion level (51.4) and the lowest average daily gain (15.6g d -1 ), and this one showed no difference compared with cupuassu (23.0g d -1 ). The residues can be used as an alternative for sheep feeding.

Resumo As concentrações de cádmio, cobalto, cromo, cobre, manganês, níquel, zinco e chumbo foram detectadas em penas de Maçarico-acanelado (Calidris subruficollis) capturados durante o período de invernada e analisados em relação a massa corporal. Destes metais analisados, somente os níveis de cobre (2,28 µg/g) foram correlacionados positivamente com a massa corporal dos indivíduos. Níveis de zinco apresentaram-se mais altos (67,97 µg/g) que outros metais e os níveis de cádmio apresentaram as menores concentrações (0,14 µg/g). As concentrações dos elementos-traços estão abaixo dos níveis de toxicidade em todos os testes químicos e sugerimos que isto provavelmente ocorre pela manutenção dos elementos essenciais através do mecanismo normal de homeostase e não reflete a exposição destes elementos durante a migração ou nas áreas de invernada.

Abstract Cadmium, cobalt, chromium, copper, manganese, nickel, zinc and lead concentrations were detected in feathers of Buff-breasted Sandpipers (Calidris subruficollis) captured during the non-breeding season and analyzed with relationship to body mass. Of these metals tested for, only copper levels (2.28 µg/g) were positively correlated with bird body mass. Zinc levels showed higher concentration (67.97 µg/g) than the other metals, and cadmium levels showed the lowest concentration (0.14 µg/g). Trace element concentrations were below toxicity levels for all tested chemicals and we suggest that this probably reflects that essential elements are maintained there by normal homeostatic mechanism and that no excessive environmental exposure to these elements during migration or on the wintering area is suggested by these results.

The objective of this study was to monitor 11 organophosphorus pesticides in samples of papaya, bell pepper, and banana, commercialized in the metropolitan area of Vitória (ES, Brazil). The pesticides were determined by an optimized and validated method using high performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). All three samples exhibited a matrix effect for most of the pesticides, mainly with signal suppression, and therefore the calibration curves were produced in matrices. Linearity revealed coefficients of determination (r2) greater than 0.9895 for all pesticides and recovery results ranged from between 76% and 118% with standard deviation no greater than 16%. Precision showed relative standard deviation values lower than 19% and HorRat values lower than 0.7, considering all pesticides. Limits of quantification were less than 0.01 mg/kg for all pesticides. Regarding analysis of the samples (50 of each), none of the pesticides exceeded the maximum residue limit determined by Brazilian legislation.

Gestão Integrada da Zona Costeira (GIZC) nos países da América Latina se encontra em diferentes níveis de desenvolvimento, desde programas estabelecidos, passando por níveis experimentais e pela não-existência de iniciativas de GIZC em alguns casos. Isso cria uma predisposição para o desenvolvimento de novas ideias e experiências, especialmente nos longos trechos de litoral, nos quais a ocupação ainda não foi planejada e/ou consolidada. Há, no entanto, questões localizadas que precisam ser atendidas de imediato, especialmente em ambientes costeiros que já foram urbanizados, industrializados ou ainda que se desenvolveram em torno de instalações portuárias. Espanha e Portugal - agora sob a influência da União Europeia - permanecem como nossos modelos de iniciativas de GIZC, tanto públicas quanto privadas. Estas oportunidades e necessidades de alguma forma refletem na produção local científica e seu impacto internacional, bem como em nossos programas de capacitação para a gestão integrada. Nós examinamos publicações de importância para gestão costeira a fim de averiguar como acadêmicos latino-americanos cumprem a sua missão de criar, desenvolver e discutir a GIZC. Analisamos também programas de capacitação e redes estabelecidas. As revistas mais representativas para a comunidade acadêmica de GIZC e que vem sendo publicadas pelo menos nos últimos 30 anos, e que foram brevemente examinadas nesta revisão foram a Ocean and Coastal Management, Coastal Management, Journal of Coastal Research e Journal of Coastal Conservation. Com relação aos programas de capacitação para a GIZC levou-se em consideração estudos recentes que demonstram que a maioria dos temas tratados nos cursos de pós-graduação acadêmicos relacionados com a GIZC não estão diretamente relacionadas com a gestão, mas sim às ciências natural, ou da terra, ou ainda à descrição da dinâmica costeira e ou poluição. Com apoio da Rede Ibero Americana de Gestão Costeira Integrada pode-se chegar a listagem de diversas redes que atuam na América Latina, Espanha e Portugal e que se relacionam com o tema. Os resultados sugerem que a representação nos principais veículos de publicação é tímido e precisa melhorar em número e diversidade de assuntos. Melhorias na publicação científica poderia levar a uma melhor utilização dos temas para o benefício dos tomadores de decisão. A capacitação também ainda está pouco desenvolvida na região. Existem poucos cursos especializados para formar técnicos e cientistas sobre o tema em níveis elevados de educação, ou nível de pós-graduação. Os programas de treinamento em torno de todos os aspectos da GIZC são ainda mais raros. A tendência em se considerar a perspectiva de integração como uma soma de conhecimentos disciplinares, não necessariamente ligados, são apresentadas em iniciativas de capacitação e produção científica. Esta abordagem é considerada falha pois, estas disciplinas, a maior parte do tempo, não se inter-relacionam para fornecer uma perspectiva holística para a gestão. Ações coordenadas, tais como redes, que ultrapassam as fronteiras políticas são uma das muitas necessidades futuras destacadas aqui como sendo a próxima fronteira para os pensadores e gestores da região.

The advancement of Integrated Coastal Zone Management (ICZM) in Latin American countries may vary from established programs to experimental phase implementation and even inexistence. This creates an opportunity for the development of new ideas and experiments, especially in the quite long stretches of littoral where no occupation has yet been planned or consolidated. However there are localized issues that need to be attended with certain degree of urgency, especially in coastal settings that have been urbanized, developed for industry and/or are situated around port facilities. Spain and Portugal implemented the marine and environmental policies of European Union and may be seen as models for public and private ICZM initiatives. These situations created opportunities and needs that are somehow reflected in dedicated scientific production, as well as in social impact and the related capacity building programs. We surveyed some key costal management journals to know how Latin American based academics are doing in their mission of creating, developing and discussing ICZM along our diverse shores stretching along all climatic zones. Capacity building programs and exchange networks were also scrutinized. Results suggest that the thematic participation in the major publication channels is modest and needs to improve in number and diversity of subjects bringing obvious benefits to the policy and decision makers. Capacity building programs are still in its infancy across the region. There are no, or very few, specialized educational programs to form practitioners and scientists at all academic levels. Training programs integrating countries around all aspects of ICZM are even scarcer. Coordinated actions, such as networks across the political borders are one of the many future needs we point out here as being the next frontier for thinkers and managers in the region.

The diagnostic usefulness of Ziehl-Neelsen (ZN)-stained sputum smears combined with conventional polymerase chain reaction (ZN/PCR) to amplify IS6110 region DNA extracted from ZN slides was evaluated. The objective was to verify if this association could improve tuberculosis (TB) diagnosis in patients at remote sites. The study was carried out in 89 patients with culture-confirmed pulmonary TB as defined by the Brazilian Manual for TB Treatment. The participants were recruited in a reference unit for TB treatment in Rondônia, a state in the Amazonian area in northern Brazil. ZN, PCR, and culture performed in the sputum samples from these patients were analyzed in different combinations (i.e., ZN plus PCR and ZN plus culture). The prevalence rates of pulmonary TB in these patients were 32.6 and 28.1% considering culture and ZN/PCR, respectively. The sensitivity and specificity of ZN/PCR were 86 and 93%, respectively. ZN/PCR was able to detect more TB cases than ZN alone. This method could offer a new approach for accurate tuberculosis diagnosis, especially in remote regions of the world where culture is not available.

We present a set of six non-linear stochastic diffferential equations for the six variables which are relevant for the dynamical behavior of the magnetic moments in ferrofluids, namely, the three Euler angles of the magnetic particle, the two polar angles of the magnetic moment relative to the particle and the modulus of the magnetic moment. The interaction between the magnetic particle and the solvent fluid is modeled by dissipative and random noise torques, and so is the interaction between the particle and its magnetic moment, treated as an independent physical entity. In the appropriate limits, the model system reduces to the cases of super-paramagnetic or of non-super-paramagnetic (blocked magnetic moments) particles. Numerical results show that for non-zero moment of inertia the precession of the magnetic moment around the magnetic field is accompanied by "nutation". It is also indicated how the dynamic complex susceptibility may be calculated from the equations of motion and the numerical results show that the nutation leads to a second resonance peak.

Amostras do vírus da Diarréia Viral Bovina (BVDV), denominadas de BVDV tipo 2 (BVDV-2), foram inicialmente identificadas em surtos de BVD aguda e enfermidade hemorrágica e têm sido isoladas predominantemente na América do Norte. O presente artigo descreve dois casos de enfermidade gastroentérica/respiratória seguidos de isolamento e identificação de amostras de BVDV tipo 2 no sul do Brasil. Os vírus foram isolados de duas novilhas de diferentes rebanhos. Um dos animais apresentou enfermidade aguda, cursando com anorexia, atonia ruminal, diarréia escura ou muco-sanguinolenta, tenesmo e descarga nasal muco-purulenta. O outro animal desenvolveu enfermidade de longa duração (7 meses), caracterizada por crescimento retardado, anorexia, quadros recorrentes de diarréia, dermatite interdigital, hemorragias digestivas e genitais ocasionais, conjuntivite, artrite e pneumonia crônica. Congestão disseminada das mucosas, ulcerações extensivas e profundas na língua, palato e esôfago, áreas necróticas na mucosa do rúmen, áreas de congestão e ulcerações cobertas com fibrina no intestino delgado foram os achados mais proeminentes. Antígenos do BVDV foram demonstrados por imunohistoquí-mica no epitélio da língua, nos pulmões e em linfonodos mesentéricos. Amostras não-citopáticas do BVDV foram isoladas em cultivo celular a partir de leucócitos e do baço dos animais afetados e identificadas por imunofluorescência. Caracterização antigênica e análise filogenética desses isolados, e de outras duas amostras de BVDV isoladas de fetos coletados em matadouros, revelou tratar-se de BVDV tipo 2. A presença do BVDV tipo 2 na população bovina do Brasil possui um significado epidemiológico importante e pode ter conseqüências para o diagnóstico, estratégias de imunização e produção de vacinas.

Highly virulent bovine viral diarrhea virus (BVDV) isolates, named BVDV type-2 (BVDV-2), were initially identified in outbreaks of acute and hemorrhagic BVD and have been previously isolated mainly in North America. The present article describes two cases of gastroenteric/respiratory disease in southern Brazil from which BVDV type 2 viruses were isolated. The viruses were isolated from two heifers belonging to different herds. One animal developed an acute disease, characterized by anorexia, ruminal atony, dark to bloody diarrhea, tenesmo and mucopurulent nasal discharge. The other animal developed a long lasting disease (7 months), characterized by retarded growth, anorexia, recurrent episodes of diarrhea, interdigital dermatitis, occasional digestive and genital bleeding, conjuntivitis, arthritis and chronic pneumonia. Disseminated mucosal congestion, extensive and deep ulcerations in the tongue, palate and esophagus, necrotic areas in the ruminal mucosa, areas of congestion covered with fibrin in the small intestine were the most prominent findings. BVDV antigens were detected by immunohistochemistry in the tongue epithelium, lungs and in mesenteric lymph nodes. Non-cytopathic BVD viruses were isolated from white blood cells and spleen from the affected animals through inoculation of cultured cells and demonstration of viral antigens by immunofluorescence. Subsequently, antigenic characterization and phylogenetic analysis of these – plus two BVD viruses that have been isolated from healthy fetuses – allowed their classification into the genotype 2. The presence of BVDV-2 among Brazilian cattle is epidemiologically relevant and may have important implications for diagnosis, immunization strategies and vaccine production.

Objetivando avaliar a eficiência da técnica de punção hepática no diagnóstico da fasciolose ovina, quer aguda, subaguda ou crônica, em regiões endêmicas, esta foi experimentada em dois grupos de animais. O grupo 1, constando de 65 deles, posteriormente sacrificados, e o grupo 2, envolvendo 12 ovinos previamente selecionados ao acaso, de um rebanho original de 250, ambos oriundos do Município de Santa Vitória do Palmar, RS. Os resultados, em ambos os grupos, corroboraram os achados dos exames coprológicos; e, em algumas amostras em que estes resultaram negativos, a positividade foi confirmada através da técnica da punção-biópsia. Demonstra-se, assim, ser esse um excelente instrumento no diagnóstico da F. hepatica, nas três fases, principalmente quando acompanhados de informações quanto à epidemiologia da doença na região.

In order to evaluate the efficacy of puncture-biopsy technique on the diagnostic of acute, sub-acute or chronic fasciolosis in sheep raised in epidemiological region, an investigation involving two groups of animals was carried out. Group 1 was made up by 65 sheep, which were later slaughterhouse, and Group 2 was made up by 12 animals, which were randomly selected from a single flock from the same area. From the sheep belonging to the Group 1, besides the biopsies, they were sampled faeces and all the livers were evaluated at slaughterhouse. Yet the animals that made up Group 2, besides biopsied, were sampled aiming F. hepatica ova detection under coprologic test. The biopsy’s material was histologically processed and stained by H.E. and Masson methods. All the animals that were positive for F. hepatica under coprologic test, as well as some that were negative, were positives to puncture-biopsy methodology. This technique proved to be an excellent toll for the diagnostic of F. hepatica infection, either acute, sub-acute or chronic. This method was also considered to be more sensitive than faecal single test, mostly in areas which parasite prevalence is known.

É relatado um caso de paralisia periódica tireotóxica. Os autores chamam a atenção para o fato de não haver registro de publicações semelhantes na literatura brasileira nos 10 últimos anos. São abordados diversos aspectos do diagnóstico diferencial com outros tipos de paralisia periódica.

A case of thyrotoxic periodic paralysis based on clinical grounds, laboratory data and therapeutic response as well is reported. The authors comment on the differential diagnosis with the most frequent types of periodic paralysis. The importance of a correct diagnosis and treatment as early as possible is stressed as to prevent further development of permanent paralysis due to irreversible degenerative myofibril changes as stated in literature.