Abstract Diabetic-metabolic syndrome (MetS-D) has a high prevalence worldwide, in which an association with the rupture of the intestinal epithelium barrier function (IEBF) has been pointed out, but the functional and morphological properties are still not well understood. This study aimed to evaluate the impact of acute hyperglycemia diabetes on intestinal tight junction proteins, metabolic failure, intestinal ion and water transports, and IEBF parameters. Diabetes was induced in male Rattus norvegicus (200-310 g) with 0.5 mL of streptozotocin (70 mg/kg). Glycemic and clinical parameters were evaluated every 7 days, and intestinal parameters were evaluated on the 14th day. The MetS-D animals showed a clinical pattern of hyperglycemia, with increases in the area of villi and crypts, lactulose:mannitol ratio, myeloperoxidase (MPO) activity, and intestinal tissue concentrations of malondialdehyde (MDA), but showed a reduction in reduced glutathione (GSH) when these parameters were compared to the control. The MetS-D group had increased secretion of Na+, K+, Cl-, and water compared to the control group in ileal tissue. Furthermore, we observed a reduction in mRNA transcript of claudin-2, claudin-15, and NHE3 and increases of SGLT-1 and ZO-1 in the MetS-D group. These results showed that MetS-D triggered intestinal tissue inflammation, oxidative stress, complex alterations in gene regulatory protein transcriptions of intestinal transporters and tight junctions, damaging the IEBF and causing hydroelectrolyte secretion. Diabeticmetabolic Diabetic MetSD MetS D (MetS-D worldwide (IEBF out understood proteins failure transports 200310 200 310 (200-31 g 05 0 5 0. 70 (7 mg/kg. mgkg mg/kg . mg kg mg/kg) days th day crypts lactulosemannitol lactulose mannitol ratio MPO (MPO activity MDA, MDA , (MDA) GSH (GSH Na Na+ K K+ Cl, Cl Cl- Furthermore claudin2, claudin2 claudin 2, 2 claudin-2 claudin15, claudin15 15, 15 claudin-15 NHE SGLT1 SGLT 1 SGLT- ZO1 ZO ZO- inflammation stress junctions 20031 20 31 (200-3 ( (MDA claudin- claudin1 claudin-1 2003 3 (200- (200 (20 (2

RESUMO O objetivo deste artigo é avaliar o efeito da folga financeira no value-relevance de gastos ambientais em firmas do mercado de capitais brasileiro. A literatura nacional tem negligenciado a abordagem de investigações sobre value-relevance de informações ambientais de desempenho, diferentemente do que ocorre na literatura estrangeira. O estudo aborda a combinação de informações ambientais com a condição financeira das firmas no escopo do value-relevance, obtendo evidências ainda não contempladas na literatura acerca do mercado de capitais brasileiro. Destaca-se que a perspectiva financeira influencia fortemente a avaliação das firmas no tocante à dimensão ambiental. A empresa que realiza gastos ambientais e detém melhor condição financeira passa a ser mais bem avaliada e considerada economicamente interessante. O mesmo não ocorre com firmas em condição financeira inferior. O estudo possibilita demonstrar como a dimensão ambiental, não prioritária na visão do shareholder, poderá ser objeto de atenção de investidores, se o requisito condição financeira é atendido. O mercado monitora gastos ambientais a fim de avaliar muito mais a extensão dos efeitos derivados desses dispêndios na geração de fluxos de caixa futuros, e menos o comprometimento ambiental da empresa. A amostra reúne 52 empresas com ações negociadas na B3 S.A. no período 2009-2018, totalizando 252 observações. Foi usado o modelo proposto por Ohlson, adaptado seguindo a literatura, e operacionalizado em modelos POLS. Ademais, análises foram reproduzidas considerando-se o possível efeito moderador das medidas de folga financeira na proxy dos gastos ambientais. Os resultados indicam que os gastos ambientais contribuem para reduzir a avaliação das firmas, sinalizando para os investidores apenas custos econômicos futuros. Observou-se que a folga financeira constitui elemento importante na avaliação das firmas com gastos ambientais, e que, nas empresas com gastos ambientais e maior folga financeira, a avaliação foi superior à daquelas com menor folga financeira. valuerelevance value relevance brasileiro desempenho estrangeira valuerelevance, relevance, Destacase Destaca interessante inferior shareholder atendido futuros 5 B SA S S.A 20092018, 20092018 2009 2018, 2018 2009-2018 25 observações Ohlson POLS Ademais considerandose considerando Observouse Observou 2009201 200 201 2009-201 2 200920 20 2009-20 20092 2009-2 2009-

ABSTRACT The objective of this article is to evaluate the effect of financial slack on the value relevance of environmental expenditures in Brazilian capital market firms. The Brazilian literature, unlike the foreign literature, has neglected to investigate the value relevance of environmental performance information. The study addresses the combination of environmental information with the financial condition of firms within the scope of value relevance, obtaining evidence not yet contemplated in the literature on the Brazilian capital market. It is noteworthy that the financial perspective strongly influences the valuation of firms in terms of the environmental dimension. Companies that engage in environmental spending and are in a better financial position are better valued and considered economically interesting. The same is not true for firms in a worse financial position. The study shows how the environmental dimension, which is not a priority from the shareholder's point of view, can be the object of investor attention if the financial condition requirement is met. The market monitors environmental expenditures in order to assess much more the extent of the effects of these expenditures on the generation of future cash flows rather than the company's environmental commitment. The sample includes 52 companies whose shares were traded on the B3 S.A. in the period 2009-2018, totaling 252 observations. The model proposed by Ohlson was used, adapted according to the literature, and operationalized in POLS models. In addition, analyses were reproduced considering the possible moderating effect of financial slack measures on the environmental spending proxy. The results indicate that environmental spending contributes to reducing the valuation of firms, signaling to investors only future economic costs. Financial slack was found to be an important element in the valuation of firms with environmental expenditures, and companies with environmental expenditures and greater financial slack had higher valuations than those with less financial slack. dimension interesting shareholders shareholder s view met companys company commitment 5 B SA S A S.A 20092018, 20092018 2009 2018, 2018 2009-2018 25 observations used models addition proxy costs 2009201 200 201 2009-201 2 200920 20 2009-20 20092 2009-2 2009-

RESUMO Com o objetivo identificar quais consórcios compostos por gramíneas de clima tropical ou temperado são mais utilizados na alimentação de ruminantes e quais resultam em maior produtividade, foi realizada uma revisão integrativa. A revisão seguiu um protocolo desenvolvido e testado de acordo com a estratégia PVO, para formular a pergunta orientadora. Os dados foram coletados em artigos publicados nas bases de dados eletrônicos SCOPUS (Elsevier), Web of Science (Coleção Principal) e SciELO Citation Index (Web of Science), por meio de uma única busca cruzada de referências. Não foi utilizado corte temporal, regional nem limitado ao Brasil. Entre os estudos selecionados, os gêneros encontrados foram Lolium (26,7%), Avena (26,7%), Brachiaria (20,0%), Cynodon (13,4%), Panicum (6,6%) e Sorghum (6,6%). As tecnologias adotadas nos artigos foram metas de altura (50,0%), ressemeadura (33,3%) e intensidade de desfolhamento (16,7%). As espécies animais utilizadas foram bovinos de corte, bovinos leiteiros e cabras - 50,0%, 33,3% e 16,7%, respectivamente. Foi possível verificar que o uso de diferentes cultivares promove resultados positivos; isso foi mais evidente com as gramíneas mais utilizadas no consórcio: azevém (Lolium multiflorum Lam) e a aveia-preta (Avena strigosa Schreb), que apresentaram valores de produtividade mais altos em comparação ao monocultivo. integrativa PVO orientadora Elsevier, Elsevier , (Elsevier) Coleção Principal Science, Science) referências temporal Brasil selecionados 26,7%, 267 26,7% 26 7 (26,7%) 20,0%, 200 20,0% 20 0 (20,0%) 13,4%, 134 13,4% 13 4 (13,4%) 6,6% 66 6 (6,6% 6,6%. . 500 50,0% 50 (50,0%) 333 33 3 (33,3% 16,7%. 167 16,7% 16 (16,7%) 33,3 respectivamente positivos consórcio Lam aveiapreta aveia preta Schreb, Schreb Schreb) monocultivo (Elsevier 26,7 2 (26,7% 20,0 (20,0% 13,4 1 (13,4% 6,6 (6,6 50,0 5 (50,0% (33,3 16,7 (16,7% 33, 26, (26,7 20, (20,0 13, (13,4 6, (6, 50, (50,0 (33, 16, (16,7 (26, (20, (13, (6 (50, (33 (16, (26 (20 (13 ( (50 (3 (16 (2 (1 (5

ABSTRACT An integrative review was conducted to identify which intercrops, composed of grasses from tropical or temperate climates, are most commonly used in ruminant feeding and which result in higher productivity. The review followed a developed and tested protocol according to the PVO strategy to formulate the guiding question. Data were collected from articles published in the electronic databases SCOPUS (Elsevier), Web of Science (Main Collection), and SciELO Citation Index (Web of Science) through a single cross-reference search. It was not carried out temporally, regionally, nor limited to Brazil. Among the selected studies, the genera found were Lolium (26.7%), Avena (26.7%), Brachiaria (20.0%), Cynodon (13.4%), Panicum (6.6%), and Sorghum (6.6%). The technologies adopted in the articles were target height (50.0%), reseeding (33.3%), and defoliation intensity (16.7%). The animal species used were beef cattle, dairy cattle, and goats - 50.0%, 33.3%, and 16.7%, respectively. It was possible to observe that the use of different cultivars yields positive results; this was most evident with the grasses most used in intercropping: ryegrass (Lolium multiflorum Lam) and black oats (Avena strigosa Schreb); which showed higher productivity values compared to monoculture. intercrops climates question Elsevier, Elsevier , (Elsevier) Main Collection, Collection Collection) crossreference cross reference search temporally regionally Brazil studies 26.7%, 267 26.7% 26 7 (26.7%) 20.0%, 200 20.0% 20 0 (20.0%) 13.4%, 134 13.4% 13 4 (13.4%) 6.6%, 66 6.6% 6 (6.6%) 6.6%. . 500 50.0% 50 (50.0%) 333 33.3% 33 3 (33.3%) 16.7%. 167 16.7% 16 (16.7%) cattle respectively results intercropping Lam Schreb Schreb) monoculture (Elsevier 26.7 2 (26.7% 20.0 (20.0% 13.4 1 (13.4% 6.6 (6.6% 50.0 5 (50.0% 33.3 (33.3% 16.7 (16.7% 26. (26.7 20. (20.0 13. (13.4 6. (6.6 50. (50.0 33. (33.3 16. (16.7 (26. (20. (13. (6. (50. (33. (16. (26 (20 (13 (6 (50 (33 (16 (2 (1 ( (5 (3

RESUMO O objetivo foi testar a inoculação com Aspergillus terreus e Trichoderma longibrachiatum sobre a fermentação, a composição bromatológica e microbiológica de silagem de capim-elefante cultivar ‘Cameroon’ (Cenchrus purpureus). Os tratamentos foram A. terreus a 105 unidades formadores de colônias (UFC)/g (AT15), T. longibrachiatum a 105 UFC/g (TL20), a mistura de ambos a 105 UFC/g (MIX), cada, e um controle não inoculado (CONTR). O delineamento foi inteiramente ao acaso, com sete repetições. A silagem MIX foi mais estável após abertura, enquanto CONTR, AT15 e TL20 apresentaram menor perda de massa seca. Não houve efeito de inoculação sobre a composição bromatológica das silagens. Apenas a silagem MIX (4,40) apresentou pH acima do mínimo de 4,2 para silagem de capim úmido e superior ao controle (4,05). Bactérias do gênero Diplococcus foram identificadas na abertura das silagens TL20 e CONTR. Após exposição ao ar, a população de bastonetes, Lactobacillus e bactérias láticas totais foram maiores em TL20 e MIX. A mistura de T. longibrachiatum e A. terreus aumenta a perda de matéria seca e o pH da silagem. T. longibrachiatum é mais eficiente em manter as populações de bactérias láticas totais após a abertura. Portanto, essa cepa tem potencial como aditivo para silagem de capim-elefante ‘Cameroon’. fermentação capimelefante elefante ‘Cameroon Cameroon Cenchrus purpureus. purpureus . purpureus) 10 UFCg UFC /g g AT15, AT , (AT15) T TL20, TL (TL20) MIX, (MIX) cada CONTR (CONTR) acaso repetições AT1 TL2 4,40 440 4 40 (4,40 42 2 4, 4,05. 405 4,05 05 (4,05) ar bastonetes Portanto ‘Cameroon. 1 (AT15 (TL20 (MIX (CONTR 4,4 44 (4,4 4,0 0 (4,05 (AT1 (TL2 (4, (4,0 (AT (TL (4 (

ABSTRACT The objective was to evaluate the inoculation with Aspergillus terreus and/or Trichoderma longibrachiatum on fermentation, chemical and microbiological composition of elephant grass ‘Cameroon’ silage (Cenchrus purpureus). Treatments were A. terreus at 105 colony forming units (CFU)/g (AT15), T. longibrachiatum at 105 CFU/g (TL20), a mixture of both at 105 CFU/g (MIX), and a control group without inoculation (CONTR). The design was completely randomized with seven replicates. The MIX silage was most stable, while CONTR, AT15, and TL20, had lower dry matter losses. There was no effect of inoculation in the chemical composition of silages. Only MIX silage (4.40) had pH above the minimum of 4.2 for humid grass silage and above the control (4.05). Bacteria from Diplococcus genus was identified at the opening of TL20 and CONTR silages. After air exposure, the population of rods, Lactobacillus, and total lactic acid bacteria was higher in theTL20 and MIX. The inclusion of a T. longibrachiatum and A. terreus mixture increases dry mater loss and silage pH. T. longibrachiatum was more efficient in maintaining populations of total lactic acid bacteria after opening; therefore, this strain has potential as an additive for elephant grass ‘Cameroon’ silage. andor or fermentation ‘Cameroon Cameroon Cenchrus purpureus. purpureus . purpureus) A 10 CFUg CFU /g g AT15 AT , (AT15) T TL (TL20) MIX, (MIX) CONTR. (CONTR) replicates stable losses silages 4.40 440 4 40 (4.40 42 2 4. 4.05. 405 4.05 05 (4.05) TL2 exposure rods Lactobacillus theTL theTL2 therefore 1 AT1 (AT15 (TL20 (MIX (CONTR 4.4 44 (4.4 4.0 0 (4.05 (AT1 (TL2 (4. (4.0 (AT (TL (4 (

RESUMO Objetivou-se avaliar a emissão de metano, a produção de leite e o comportamento de ovelhas mantidas exclusivamente em pastagem irrigada de Tifton 85 (Cynodon spp.) ou suplementadas com concentrados à base de milho grão ou caroço de algodão. Doze ovelhas Lacaune x Santa Inês (43,07±0,83 kg de peso corporal e 77±24 dias após a parição, em média) foram distribuídas em quadrados latinos 3x3 replicados. Os tratamentos consistiram em três dietas: pastagem (sem suplementação concentrada); milho (pastagem + suplemento à base de milho); caroço de algodão integral (pastagem + suplemento à base de caroço de algodão), oferecendo 0,5 kg/ovelha/dia. O grupo caroço de algodão apresentou maior consumo de matéria seca (CMS) (P=0,049) e consumo de proteína bruta (PB) (P=0,001) em relação aos demais. Não houve diferença no CMS total (P=0,115) para as dietas testadas. Os animais exclusivamente em pastagem apresentaram maior consumo de forragem (P=0,004), menor digestibilidade da PB (P=0,015), maior tempo em pastejo (P=0,01) e menor tempo em ócio (P=0,01), em relação aos grupos suplementados. A produção de leite (0,36 kg/ovelha/dia) (P=0,15) e a emissão de metano (33,12 g/ovelha/dia) (P=0,95) foram semelhantes nos três grupos avaliados. A suplementação com concentrado à base de milho grão ou caroço de algodão não melhorou o desempenho produtivo e não reduziu a emissão de metano. Entretanto, as ovelhas lactantes mantidas exclusivamente em pastagem apresentaram maior tempo em pastejo, sem alterações na produção de leite e na emissão de metano. Objetivouse Objetivou se 8 Cynodon spp. spp 43,07±0,83 4307083 43 07 0 83 (43,07±0,8 7724 77 24 77±2 parição média 3x replicados concentrada concentrada) milho) algodão, , algodão) 05 5 0, kgovelhadia ovelha dia kg/ovelha/dia (CMS P=0,049 P0049 P 049 (P=0,049 (PB P=0,001 P0001 001 (P=0,001 demais P=0,115 P0115 115 (P=0,115 testadas P=0,004, P0004 P=0,004 004 (P=0,004) P=0,015, P0015 P=0,015 015 (P=0,015) P=0,01 P001 01 (P=0,01 P=0,01, suplementados 0,36 036 36 (0,3 P=0,15 P015 15 (P=0,15 33,12 3312 33 12 (33,1 g/ovelha/dia govelhadia g P=0,95 P095 95 (P=0,95 avaliados Entretanto 43,07±0,8 430708 4 (43,07±0, 772 7 2 77± P=0,04 P004 04 (P=0,04 P=0,00 P000 00 (P=0,00 P=0,11 P011 11 (P=0,11 (P=0,004 (P=0,015 P=0,0 P00 (P=0,0 0,3 03 3 (0, P=0,1 P01 1 (P=0,1 33,1 331 (33, P=0,9 P09 9 (P=0,9 43,07±0, 43070 (43,07±0 P=0, P0 (P=0, (0 33, (33 43,07±0 4307 (43,07± P=0 (P=0 ( (3 43,07± 430 (43,07 P= (P= 43,07 (43,0 (P 43,0 (43, 43, (43 (4

ABSTRACT This study aimed to evaluate methane emission, milk yield and behavior of ewes kept exclusively on irrigated pasture of Tifton 85 grass (Cynodon spp.) or supplemented with ground corn or whole cottonseed (WCS) based concentrates. Twelve Lacaune x Santa Ines ewes (43.07±0.83 kg of body weight, 77±24 days after parturition, on average) were distributed in replicated 3x3 Latin square. Treatments consisted of three diets: pasture (no concentrate supplementation); corn (pasture + corn-based supplement); whole cottonseed (pasture + whole cottonseed-based supplement), offering 0.5 kg/ewe/day. The WCS group showed the highest concentrate dry matter intake (DMI) (p=0.049) and crude protein (CP) intake (p=0.001) compared to the others. There was no difference on total DMI (p=0.115) for the tested diets. Animals exclusively kept on pasture had the greatest forage DMI (p=0.004), lowest CP digestibility (p=0.015), showed longer grazing time (p=0.01) and shorter idle time (p=0.01) compared to the supplemented groups. Milk yield (0.36 kg/ewe/day) (p=0.15) and methane emission (33.12 g/ewe/day) (p=0.95) were similar for all three evaluated groups. Supplementation with concentrate based on corn or whole cottonseed does not improve productive performance nor decrease methane emission. However, lactating ewes kept exclusively in pasture show longer grazing time, without changes in milk yield and methane emission. 8 Cynodon spp. spp (WCS concentrates 43.07±0.83 4307083 43 07 0 83 (43.07±0.8 weight 7724 77 24 77±2 parturition average 3x square diets supplementation supplementation) cornbased supplement supplement) cottonseedbased supplement, , 05 5 0. kgeweday ewe day kg/ewe/day (DMI p=0.049 p0049 p 049 (p=0.049 (CP p=0.001 p0001 001 (p=0.001 others p=0.115 p0115 115 (p=0.115 p=0.004, p0004 p=0.004 004 (p=0.004) p=0.015, p0015 p=0.015 015 (p=0.015) p=0.01 p001 01 (p=0.01 groups 0.36 036 36 (0.3 p=0.15 p015 15 (p=0.15 33.12 3312 33 12 (33.1 g/ewe/day geweday g p=0.95 p095 95 (p=0.95 However 43.07±0.8 430708 4 (43.07±0. 772 7 2 77± p=0.04 p004 04 (p=0.04 p=0.00 p000 00 (p=0.00 p=0.11 p011 11 (p=0.11 (p=0.004 (p=0.015 p=0.0 p00 (p=0.0 0.3 03 3 (0. p=0.1 p01 1 (p=0.1 33.1 331 (33. p=0.9 p09 9 (p=0.9 43.07±0. 43070 (43.07±0 p=0. p0 (p=0. (0 33. (33 43.07±0 4307 (43.07± p=0 (p=0 ( (3 43.07± 430 (43.07 p= (p= 43.07 (43.0 (p 43.0 (43. 43. (43 (4

Abstract This paper presents an equipment for monitoring synchronous generators condition through characteristics of the time derivative of the external magnetic field. The developed monitoring methodology allows the identification of established or incipient faults, by detecting changes in the magnetic signature of the synchronous generator. In this methodology, the measurement of signals outside the machine gives this equipment a non-invasive characteristic, allowing its monitoring without interfering or disturbing its operation. The developed system includes the specification of magnetic field sensors, signal measurement and processing equipment, as well as software for analysis and monitoring. The validation of the methodology used in this system was carried out through the analysis of experimental data, presenting efficient results in the detection of electrical and mechanical faults in synchronous generators of an experimental test bench and a hydroelectric power plant. As a result, the commercial specification of this equipment was obtained and two units were implemented in a hydroelectric power plant to monitor 305 MVA synchronous generators.

The first objective of this study was to assess Marandu palisade grass in an integrated crop-livestock system (ICL), and two integrated crop-livestock-forest (ICLF) systems. The first ICLF had a single row of eucalyptus (ICLF-1L) and the second had three rows of eucalyptus (ICLF-3L). The second objective was to assess the performance, behaviour, and thermal comfort of Nellore cattle in these systems during summer and winter. The data were collected for two years after the systems were implemented. Sixty Nellore cattle were used. Dry forage mass (DFM) was similar in all systems with a higher percentage of leaves observed in ICLF-3L in winter compared with ICL. Forage in the ICLF-1L and ICLF-3L systems contained more crude protein (CP) than ICL. The radiant thermal load was lower for ICLF-1L and ICLF-3L, with a lower globe temperature in ICLF-3L. The stocking rate was lowest for ICLF-3L. Two years after the systems had been established, the trees provided low density shade and implementing the ICLF-1L and ICLF-3L systems improved the CP content of the grass without changing the levels of DFM and performance of the cattle. The trees reduced the total thermal energy exchanged between the animal and the environment, improving thermal comfort, but did not influence cattle behaviour or their haematological parameters.

Abstract Currently, antimicrobial resistance has become a global public health problem, which has made the need for new antimicrobial compounds to deal with resistant infections an emergency. However, environments that once offered so many innovative molecules, now already exhaustively exploited, do not meet this need. In this context, a geographically isolated, under-explored and extreme environment, such as Antarctica, which holds organisms with unique physiological and biochemical characteristics, assumes great importance as a potential source of new compounds with antimicrobial activity. In this patent review, we investigate the state of technological development in the field of antimicrobial compounds obtained from Antarctic organisms, highlighting the main countries and researchers active in the field, the species utilized, the compounds obtained, and their possible therapeutic applications. As results, few patent documents were found, however they encompass a wide diversity of compounds and species, indicating a great antimicrobial potential present in Antarctic biota, including compounds active against the most important human pathogenic microorganisms, such as including methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus spp. and multi-resistant Mycobacterium tuberculosis. Furthermore, due to the increasing trend in patent applications, a significant rise in the number of patents in this area is expected in the coming years.

RESUMO: O objetivo deste estudo foi determinar a frequência dos fenótipos eritrocitários do sistema AB felino e verificar a presença de outros antígenos, não pertencentes ao sistema AB felino, em gatos domésticos das cidades de Ilhéus e Itabuna, Bahia, Brasil. Amostras de sangue de 300 gatos foram coletadas no Hospital Veterinário da Universidade Estadual de Santa Cruz (UESC) e em visitas domiciliares para realizar a fenotipagem sanguínea usando o método de tubo. A fenotipagem reversa foi realizada em gatos que testaram o fenótipo B com amostras que testaram o fenótipo A, para confirmação do fenótipo sanguíneo. O teste cruzado foi realizado entre gatos do fenótipo A, para pesquisar a presença de diferentes antígenos do sistema AB dentro desse fenótipo sanguíneo. Os resultados foram submetidos a análises macroscópicas e microscópicas. Dos 300 animais testados, 110 eram machos e 190 fêmeas, e suas idades variaram de cinco meses à 15 anos. Sobre as raças, 290 eram gatos sem raça definida e dos 10 restantes, cinco eram Persas, quatro eram Siameses e um Angorá. 297 (99%) apresentaram fenótipo A (incluindo todos os gatos de raça) e três (1%) tiveram fenótipo B, sendo todos esses gatos sem raça definida. Nenhum (0%) dos gatos apresentou fenótipo AB. Todos os sangues com fenótipo B reagiram na fenotipagem reversa com o fenótipo A, confirmando o fenótipo B desses gatos. Todos os sangues com fenótipo A foram compatíveis entre si, portanto nenhum antígeno eritrocitário adicional foi detectado através deste teste. A genitora de um dos gatos com fenótipo B, foi encontrada e a mesma possuía fenótipo A, demonstrando pais com fenótipo A e cria com fenótipo B. Esse achado indica heterozigose na população estudada. Esses dados levam à conclusão de que diferentes fenótipos eritrocitários estão presentes na população estudada e destacam a importância da realização de testes fenotípicos nesses animais antes dos procedimentos de transfusão, a fim de evitar complicações hemolíticas graves decorrentes do envolvimento de animais incompatíveis.

ABSTRACT: This study aimed to determine the erythrocyte phenotypes of the feline AB system and to check the presence of antigens other than those present in the feline AB system in domestic cats from Ilhéus-Itabuna microregion, Bahia, Brazil. Three-hundred feline blood samples were collected at the Veterinary Hospital of the “Universidade Estadual de Santa Cruz” (UESC) and in home visits to perform blood phenotyping using the tube-method testing. The reverse phenotyping was made between cats that tested phenotype B with blood samples of cats that tested phenotype A to confirm the blood phenotype B. The cross-tested among cats with phenotype A was made in order to verify the presence of different antigens of AB system in this blood phenotype. The results underwent macroscopic and microscopic analyses. Among the 300 animals tested, regarding breed, 290 were mixed-breed cats and among the remaining ten, five were Persians, four Siamese, and one Angora. 297 (99%) presented with phenotype A (including all the breeding cats) and three (1%) with phenotype B, and all this cats were mixed-breed cats. None (0%) of the cats showed the phenotype AB. All phenotype B bloods reacted to reverse phenotyping with phenotype A, confirming the phenotype B of these cats. All phenotype A bloods were compatible among each other, so no further erythrocyte antigens were detected through this test. The mother of one of the phenotype B cats was identified and had phenotype A, demonstrating phenotype A parents with phenotype B offspring. This finding indicates heterozygosis in the studied population. This data enable to conclude that the studied population presented different erythrocyte phenotypes, subsequently highlighting the importance of conducting phenotype analyses in these animals before performing blood transfusion to avoid serious hemolytic complications associated with incompatibility.

ABSTRACT This study aimed to evaluate the neutrophil oxidative metabolism and phagocytosis of Zymonsan particles of primiparous and pluriparous Lacaune ewes during the first 30 days after lambing. A total of 20 ewes were evaluated, 10 primiparous (GPR) and 10 pluriparous (GPL). Evaluation of basal oxidative metabolism was performed using the nitroblue tetrazolium (NBT) technique, stimulating neutrophil phagocytosis with Zymosan particles. Blood samples were collected at parturition day (M1) and 1, 3, 7, 15 and 30 days after parturition, corresponding to M2, M3, M4, M5 and M6, respectively. In relation to the groups, GPR presented lower oxidative basal metabolism neutrophils in M1 compared to M4, in M3 with M1, M2, M4 and M5. In M4 and M5 differences were found at all times and in M6 with M4 and M5. Higher percentage of neutrophils than phagocytes were found in M4, M5 and M6 than in M1 and M2 in GPL animals. At all times GPR presented a lower percentage of phagocytosis than GPL. Thus it is concluded that the immune response in pluriparous sheep was more efficient than in primiparous sheep.

RESUMO Este trabalho tem por objetivo descrever um caso de carcinoma espinocelular da base do chifre, bilateral e simétrico, em uma vaca e discutir a questão hormonal, possivelmente envolvida na patogênese da doença. Tratava-se de uma fêmea bovina, 11 anos, com histórico de emagrecimento progressivo, presença de massas exofíticas na base de ambos os chifres e em anestro por muitos anos. Foi realizada biopsia incisional de ambas as lesões para exame histopatológico e dosagens hormonais. Devido à progressão do quadro clínico, optou-se pela eutanásia, seguida dos exames necroscópico e histopatológico. O exame histopatológico revelou tratar-se de um carcinoma espinocelular infiltrativo bem diferenciado, e as dosagens hormonais apresentaram alterações nos níveis do hormônio luteinizante, folículo estimulante e estrógenos totais. Apesar de existirem descrições de carcinomas espinocelulares da base do chifre, ainda não havia relatos da ocorrência do mesmo bilateral e em uma vaca com distúrbios hormonais.

ABSTRACT The objective of this study was to describe a case of bilateral and symmetrical squamous cell carcinoma from the horn base in a cow and to discuss the hormonal question, possibly involved in its pathogenesis. A 11-year-old beef cow presenting a history of progressive thinning, presence of exophytic masses at the base of both horns and anestrous for many years was assisted. An incisional biopsy of both lesions was performed for histopathological examination and hormonal dosages. Due to the clinical progression, euthanasia followed by necroscopic and histopathological examination was carried out. Histopathological examination revealed a well differentiated infiltrative squamous cell carcinoma and the hormonal dosages presented changes in luteinizing hormone, follicle stimulating and total estrogen levels. Although there are descriptions of basal squamous cell carcinoma of the horn, there were still no reports of the occurrence of the same bilateral in a cow with hormonal disorders.

RESUMO O presente artigo teve como objetivo aprofundar discussões do desastre ocorrido em Brumadinho (MG), em janeiro de 2019. A partir da relatoria do seminário ‘Desastre da Vale S.A. em Brumadinho: seis meses de impacto e ações’, seis alunas do Curso de Especialização em Saúde Pública da Fundação Oswaldo Cruz (Fiocruz) e uma pós-doutoranda da Fiocruz de Minas Gerais realizaram transcrições de falas feitas durante o evento na tentativa de reportar essa vivência mediante lugares de fala distintos. Experiências artísticas retratadas ao longo do texto estabelecem um vínculo com a subjetividade dos atingidos e seu entorno. Portanto, o artigo pretendeu seguir esse caminho.

ABSTRACT The purpose of this article was to deepen discussions on the disaster that occurred in Brumadinho (MG), in January 2019. From the report of the seminar ‘Disaster of Vale S.A. in Brumadinho: six months of impact and actions’, six students from the Specialization Course in Public Health at Oswaldo Cruz Foundation (Fiocruz) and a postdoctoral fellow at Fiocruz in Minas Gerais performed transcriptions of speeches made during the event in an attempt to report this experience through different places of speech. Artistic experiences portrayed throughout the text establish a link with the subjectivity of those affected and their surroundings. Therefore, the article intended to follow that path.

RESUMO O presente artigo teve como objetivo aprofundar discussões do desastre ocorrido em Brumadinho (MG), em janeiro de 2019. A partir da relatoria do seminário ‘Desastre da Vale S.A. em Brumadinho: seis meses de impacto e ações’, seis alunas do Curso de Especialização em Saúde Pública da Fundação Oswaldo Cruz (Fiocruz) e uma pós-doutoranda da Fiocruz de Minas Gerais realizaram transcrições de falas feitas durante o evento na tentativa de reportar essa vivência mediante lugares de fala distintos. Experiências artísticas retratadas ao longo do texto estabelecem um vínculo com a subjetividade dos atingidos e seu entorno. Portanto, o artigo pretendeu seguir esse caminho.

ABSTRACT The purpose of this article was to deepen discussions on the disaster that occurred in Brumadinho (MG), in January 2019. From the report of the seminar ‘Disaster of Vale S.A. in Brumadinho: six months of impact and actions’, six students from the Specialization Course in Public Health at Oswaldo Cruz Foundation (Fiocruz) and a postdoctoral fellow at Fiocruz in Minas Gerais performed transcriptions of speeches made during the event in an attempt to report this experience through different places of speech. Artistic experiences portrayed throughout the text establish a link with the subjectivity of those affected and their surroundings. Therefore, the article intended to follow that path.

Abstract Periodontal disease is considered the main oral cavity disorder in dogs. Essential oils have the potential for use in the prevention and treatment of oral diseases. The antimicrobial activity of Schinus molle L. essential oil (SMEO) has already been reported. Chitosan, a natural product with antimicrobial activity and good biocompatibility has potential in biodental applications. In this study, we evaluated the in vitro antimicrobial activity of SMEO against bacteria associated with periodontal disease in dogs, developed and evaluated the physicochemical properties of a novel chitosan-based buccal delivery system containing SMEO. SMEO showed antimicrobial activity against Gram positive and Gram negative bacteria associated with canine periodontitis, with MIC values of 750 µg.mL-1 for Staphylococcus spp. and Streptococcus spp, 1000 µg.mL-1 for Corynebacterium spp. and 1250 µg.mL-1 for Pseudomonas spp. All formulations evaluated presented adequate physicochemical properties, good stability, and pH values close to buccal pH (5.0–7.0). Chitosan gel loaded with SMEO showed potential as a SMEO delivery system, having the ideal physicochemical and rheological properties (pseudoplastic and apparent viscosities) required for application on buccal tissue. Thus, we can conclude that formulation has the potential to be used for buccal mucosa delivery in the prevention and treatment of periodontal disease in dogs.

RESUMO A exploração de petróleo em terra é uma das principais atividades econômicas da região semiárida (Caatinga) do estado do Rio Grande do Norte. Um dos impactos ambientais desta atividade constitui a exploração mineral da piçarra, tipo de cascalho utilizado no embasamento de instalações, como bases de poços, e na construção de estradas de acesso. Ao final da exploração, as jazidas de piçarra e demais áreas descomissionadas, onde a piçarra foi depositada, devem ser revegetadas com espécies nativas do bioma. O plantio de espécies leguminosas, capazes de realizar associações com rizóbios e fungos micorrízicos arbusculares (FMA), tem se mostrado uma estratégia eficiente para revegetação de áreas degradadas. Entretanto, o impacto das atividades de exploração sobre as populações autóctones desses microrganismos é desconhecido. Esse estudo objetivou caracterizar a densidade de rizóbios e de esporos de FMA em quatro áreas impactadas pela exploração de petróleo no RN em relação ao presente em áreas adjacentes não impactadas. Amostras de piçarra foram coletadas nas estações seca e chuvosa em duas jazidas, uma base de poço e uma central de resíduos. Paralelamente, amostras de solo superficial (topsoil) foram coletadas em áreas adjacentes com vegetação nativa da Caatinga. Ensaios foram conduzidos visando obter o número mais provável (NMP) de rizóbios e a densidade e identificação de fungos micorrízicos arbusculares (FMA). Utilizou-se como plantas-isca de rizóbios Mimosa tenuiflora (Mart.) Benth. e Mimosa caesalpiniifolia Benth. O NMP de rizóbios mostrou-se baixo em todas as áreas, sendo superior no período chuvoso. As maiores quantidade e diversidade de esporos de FMA foram obtidas no período seco. Dos microrganismos simbiontes estudados, os FMA apresentaram-se em baixas densidades nas áreas de exploração mineral em relação às de vegetação nativa, refletindo o impacto dessa atividade.

ABSTRACT On-shore oil exploration is one of the main economic activities in the semiarid region (Caatinga biome) of the state of Rio Grande do Norte (RN), Brazil. Gravel mining is an activity associated with oil exploration that causes environmental impacts. Gravel is a base-material for constructions, such as roads and pumpjack bases. The areas of gravel mining and other decommissioned areas where the gravel has been deposited must be revegetated with species native to the biome at end of the activity in the site. An efficient strategy for revegetation of degraded areas has been the planting of leguminous species that can associate with rhizobia and arbuscular mycorrhizal fungi (AMF). Nevertheless, the impact of mining activities on the autochthonous populations of these microorganisms is unknown. The objective of the present work is to characterize the density of rhizobia and AMF spores in four areas impacted by the oil exploration in RN when compared to non-impacted adjacent areas. Gravel samples were collected in dry and rainy seasons in two mining areas: one in a pumpjack base, and one in a waste disposal area. Surface soil (topsoil) samples were collected in adjacent areas with native Caatinga vegetation. Assays were carried out to evaluate the most probable number (MPN) of rhizobia and to identify the type and density of arbuscular mycorrhizal fungi (AMF), using rhizobia trap plants Mimosa tenuiflora (Mart.) Benth. and Mimosa caesalpiniifolia Benth. The MPN of rhizobia was low in all areas, but higher in the rainy season. The highest amount and diversity of AMF spores were found in the dry season. Among the symbiotic microorganisms studied, the AMF presented lower densities in the evaluated areas when compared to those in the adjcent native vegetation areas, denoting the impact of this activity.