RESUMO A brucelose é uma importante zoonose de proporções mundiais. Os testes sorológicos utilizados no Brasil, antígeno acidificado tamponado (RBT), soroaglutinação lenta/2-mercaptoetanol (2-ME), polarização fluorescente (FPA) e fixação de complemento (FC), são baseados no antígeno lipopolissacarídeo liso (S-LPS) da Brucella abortus. O objetivo deste estudo foi avaliar uma proteína BP26 recombinante utilizada como antígeno em um teste rápido imunocromatográfico de fluxo lateral (rBP26-LFIA) para o diagnóstico sorológico da brucelose bovina. As características de desempenho analítico foram avaliadas em amostras de soro bovino positivas e negativas previamente caracterizadas pelos testes RBT e 2-ME. As estimativas da sensibilidade e especificidade analíticas foram de 73,91% e 97,14%, respectivamente. Soros bovinos reagentes à Neospora, Trypanossoma vivax e Leptospira foram utilizados para avaliação de especificidade, em razão de essas doenças serem causas frequentes de aborto em bovinos, além de ser possível reação cruzada induzida por vacinas comerciais contra Leptospira, em testes sorológicos para a brucelose bovina que utilizam S-LPS como antígeno. Como conclusão, o teste rBP26-LFIA, com sua atual padronização, apresentou um bom desempenho analítico. Contudo, uma futura avaliação do desempenho diagnóstico do teste rBP26-LFIA com amostras provenientes de regiões com prevalência conhecidas é necessária para sua recomendação de uso no programa brasileiro de controle e erradicação da brucelose bovina. mundiais Brasil RBT, , (RBT) lenta/2mercaptoetanol lenta2mercaptoetanol lentamercaptoetanol lenta/2 mercaptoetanol lenta 2 2ME, 2ME ME (2-ME) FPA (FPA FC, FC (FC) SLPS S LPS (S-LPS abortus BP BP2 rBP26LFIA rBPLFIA rBP26 LFIA rBP (rBP26-LFIA 2ME. ME. 2-ME 7391 73 91 73,91 9714 97 14 97,14% respectivamente Neospora conclusão rBP26LFIA, LFIA, padronização Contudo (RBT 2mercaptoetanol lenta2 lenta/ (2-ME (FC rBP2 739 7 9 73,9 971 1 97,14 73, 97,1 97,

ABSTRACT Brucellosis is an important bacterial disease of global distribution with zoonotic potencial. Serological tests used in Brazil for diagnosis of bovine brucellosis, including the Rose Bengal test (RBT), 2-mercaptoethanol (2-ME), fluorescent polarization (FPA), and complement fixation (FC), are based on the smooth lipopolysaccharide antigen (S-LPS) of Brucella abortus. The aim of this study was to evaluate a recombinant BP26 protein used as antigen in a rapid lateral flow immunochromatographic assay (rBP26-LFIA) for serological diagnosis of bovine brucellosis. Analytical performance of rBP26-LFIA was evaluated in positive and negative bovine serum samples previously characterized by RBT and 2-ME. Estimates of analytical sensitivity and specificity were 73.91% and 97.14%, respectively. Bovine sera reactive to Neospora, Trypanosoma vivax or Leptospira were used to assess specificity because these diseases are commonly associated with abortion in cattle. In addition to a possible cross-reaction induced by commercial vaccines against Leptospira in serological tests for bovine brucellosis using S-LPS as an antigen. In conclusion, rBP26-LFIA, with its current standardization, had good analytical performance. However, a future evaluation of diagnostic performance by rBP26-LFIA with samples from regions with known prevalence is necessary for its recommendation for use in the Brazilian program for the control and eradication of bovine brucellosis. potencial RBT, , (RBT) 2mercaptoethanol mercaptoethanol 2 2ME, 2ME ME (2-ME) FPA, FPA (FPA) FC, FC (FC) SLPS S LPS (S-LPS abortus BP BP2 rBP26LFIA rBPLFIA rBP26 LFIA rBP (rBP26-LFIA 2ME. ME. 2-ME 7391 73 91 73.91 9714 97 14 97.14% respectively Neospora cattle crossreaction cross reaction conclusion rBP26LFIA, LFIA, standardization However (RBT (2-ME (FPA (FC rBP2 739 7 9 73.9 971 1 97.14 73. 97.1 97.

ABSTRACT This study aimed to evaluate methane emission, milk yield and behavior of ewes kept exclusively on irrigated pasture of Tifton 85 grass (Cynodon spp.) or supplemented with ground corn or whole cottonseed (WCS) based concentrates. Twelve Lacaune x Santa Ines ewes (43.07±0.83 kg of body weight, 77±24 days after parturition, on average) were distributed in replicated 3x3 Latin square. Treatments consisted of three diets: pasture (no concentrate supplementation); corn (pasture + corn-based supplement); whole cottonseed (pasture + whole cottonseed-based supplement), offering 0.5 kg/ewe/day. The WCS group showed the highest concentrate dry matter intake (DMI) (p=0.049) and crude protein (CP) intake (p=0.001) compared to the others. There was no difference on total DMI (p=0.115) for the tested diets. Animals exclusively kept on pasture had the greatest forage DMI (p=0.004), lowest CP digestibility (p=0.015), showed longer grazing time (p=0.01) and shorter idle time (p=0.01) compared to the supplemented groups. Milk yield (0.36 kg/ewe/day) (p=0.15) and methane emission (33.12 g/ewe/day) (p=0.95) were similar for all three evaluated groups. Supplementation with concentrate based on corn or whole cottonseed does not improve productive performance nor decrease methane emission. However, lactating ewes kept exclusively in pasture show longer grazing time, without changes in milk yield and methane emission. 8 Cynodon spp. spp (WCS concentrates 43.07±0.83 4307083 43 07 0 83 (43.07±0.8 weight 7724 77 24 77±2 parturition average 3x square diets supplementation supplementation) cornbased supplement supplement) cottonseedbased supplement, , 05 5 0. kgeweday ewe day kg/ewe/day (DMI p=0.049 p0049 p 049 (p=0.049 (CP p=0.001 p0001 001 (p=0.001 others p=0.115 p0115 115 (p=0.115 p=0.004, p0004 p=0.004 004 (p=0.004) p=0.015, p0015 p=0.015 015 (p=0.015) p=0.01 p001 01 (p=0.01 groups 0.36 036 36 (0.3 p=0.15 p015 15 (p=0.15 33.12 3312 33 12 (33.1 g/ewe/day geweday g p=0.95 p095 95 (p=0.95 However 43.07±0.8 430708 4 (43.07±0. 772 7 2 77± p=0.04 p004 04 (p=0.04 p=0.00 p000 00 (p=0.00 p=0.11 p011 11 (p=0.11 (p=0.004 (p=0.015 p=0.0 p00 (p=0.0 0.3 03 3 (0. p=0.1 p01 1 (p=0.1 33.1 331 (33. p=0.9 p09 9 (p=0.9 43.07±0. 43070 (43.07±0 p=0. p0 (p=0. (0 33. (33 43.07±0 4307 (43.07± p=0 (p=0 ( (3 43.07± 430 (43.07 p= (p= 43.07 (43.0 (p 43.0 (43. 43. (43 (4

RESUMO Objetivou-se avaliar a emissão de metano, a produção de leite e o comportamento de ovelhas mantidas exclusivamente em pastagem irrigada de Tifton 85 (Cynodon spp.) ou suplementadas com concentrados à base de milho grão ou caroço de algodão. Doze ovelhas Lacaune x Santa Inês (43,07±0,83 kg de peso corporal e 77±24 dias após a parição, em média) foram distribuídas em quadrados latinos 3x3 replicados. Os tratamentos consistiram em três dietas: pastagem (sem suplementação concentrada); milho (pastagem + suplemento à base de milho); caroço de algodão integral (pastagem + suplemento à base de caroço de algodão), oferecendo 0,5 kg/ovelha/dia. O grupo caroço de algodão apresentou maior consumo de matéria seca (CMS) (P=0,049) e consumo de proteína bruta (PB) (P=0,001) em relação aos demais. Não houve diferença no CMS total (P=0,115) para as dietas testadas. Os animais exclusivamente em pastagem apresentaram maior consumo de forragem (P=0,004), menor digestibilidade da PB (P=0,015), maior tempo em pastejo (P=0,01) e menor tempo em ócio (P=0,01), em relação aos grupos suplementados. A produção de leite (0,36 kg/ovelha/dia) (P=0,15) e a emissão de metano (33,12 g/ovelha/dia) (P=0,95) foram semelhantes nos três grupos avaliados. A suplementação com concentrado à base de milho grão ou caroço de algodão não melhorou o desempenho produtivo e não reduziu a emissão de metano. Entretanto, as ovelhas lactantes mantidas exclusivamente em pastagem apresentaram maior tempo em pastejo, sem alterações na produção de leite e na emissão de metano. Objetivouse Objetivou se 8 Cynodon spp. spp 43,07±0,83 4307083 43 07 0 83 (43,07±0,8 7724 77 24 77±2 parição média 3x replicados concentrada concentrada) milho) algodão, , algodão) 05 5 0, kgovelhadia ovelha dia kg/ovelha/dia (CMS P=0,049 P0049 P 049 (P=0,049 (PB P=0,001 P0001 001 (P=0,001 demais P=0,115 P0115 115 (P=0,115 testadas P=0,004, P0004 P=0,004 004 (P=0,004) P=0,015, P0015 P=0,015 015 (P=0,015) P=0,01 P001 01 (P=0,01 P=0,01, suplementados 0,36 036 36 (0,3 P=0,15 P015 15 (P=0,15 33,12 3312 33 12 (33,1 g/ovelha/dia govelhadia g P=0,95 P095 95 (P=0,95 avaliados Entretanto 43,07±0,8 430708 4 (43,07±0, 772 7 2 77± P=0,04 P004 04 (P=0,04 P=0,00 P000 00 (P=0,00 P=0,11 P011 11 (P=0,11 (P=0,004 (P=0,015 P=0,0 P00 (P=0,0 0,3 03 3 (0, P=0,1 P01 1 (P=0,1 33,1 331 (33, P=0,9 P09 9 (P=0,9 43,07±0, 43070 (43,07±0 P=0, P0 (P=0, (0 33, (33 43,07±0 4307 (43,07± P=0 (P=0 ( (3 43,07± 430 (43,07 P= (P= 43,07 (43,0 (P 43,0 (43, 43, (43 (4

ABSTRACT This study characterized 30 MRSA isolates from intensive care unit (ICU) environment and equipment surfaces and healthy children. The SCCmec types I, IVa and V were detected in HA-MRSA isolates while CA-MRSA showed the SCCmec type IVa and V. Most isolates were classified as agr group II. All isolates presented the sei gene, and only HA-MRSA were positive for etb e tst genes. Three genotypes were related to Pediatric (ST5/SCCmecIV) and Berlin (ST45/SCCmecIV) clones. The present study showed molecular similarity between CA- and HA-MRSA isolates in hospital and community settings in a Brazilian region.

HIV infection has a broad spectrum of renal manifestations. This study examined the clinical and histological manifestations of HIV-associated renal disease, and predictors of renal outcomes. Sixty-one (64% male, mean age 45 years) HIV patients were retrospectively evaluated. Clinical presentation and renal histopathology were assessed, as well as CD4 T-cell count and viral load. The predictive value of histological lesion, baseline CD4 cell count and viral load for end-stage renal disease (ESRD) or death were determined using the Cox regression model. The outcomes of chronic kidney disease (CKD) and ESRD or death were evaluated by baseline CD4 cell count. The percent distribution at initial clinical presentation was non-nephrotic proteinuria (54%), acute kidney injury (28%), nephrotic syndrome (23%), and chronic kidney disease (22%). Focal segmental glomerulosclerosis (28%), mainly the collapsing form (HIVAN), acute interstitial nephritis (AIN) (26%), and immune complex-mediated glomerulonephritis (ICGN) (25%) were the predominant renal histology. Baseline CD4 cell count ≥200 cells/mm3 was a protective factor against CKD (hazard ratio=0.997; 95%CI=0.994-0.999; P=0.012). At last follow-up, 64% of patients with baseline CD4 ≥200 cells/mm3 had eGFR >60 mL·min-1·(1.73 m2)-1 compared to the other 35% of patients who presented with CD4 <200 cells/mm3 (log rank=9.043, P=0.003). In conclusion, the main histological lesion of HIV-associated renal disease was HIVAN, followed by AIN and ICGN. These findings reinforce the need to biopsy HIV patients with kidney impairment and/or proteinuria. Baseline CD4 cell count ≥200 cells/mm3 was associated with better renal function after 2 years of follow-up.

We report the case of a man with a diagnosis of invasive gastric adenocarcinoma associated with scattered osteoblastic metastases. This 65-year-old patient was admitted with nausea, vomiting and abdominal pain, in addition to loss of weight (13 Kg in 3 months). Upper digestive endoscopy revealed extensive infiltrating changes in the body of the stomach, and histopathologic data confirmed the diagnosis of a Borrmann IV gastric adenocarcinoma. Complementary exams were indicative of a gastric cancer with bone metastases, characterized by disseminated osteoblastic images of the scintigraphy of the skeleton. Palliative chemotherapy was the treatment of first choice. High serum levels of CA 19-9, CEA and alkaline phosphatase can constitute useful indicative tools of this challenging condition. Images from a scintigraphy study of the skeleton can characterize the type and extension of bone metastases. The aim of this case study is to report a case of a very uncommon condition, considering that disseminated osteoblastic metastases rarely develop in this kind of tumor.

Presentamos un caso de adenocarcinoma gástrico infiltrante asociado con metástasis osteoblásticas diseminadas. Un paciente de 65 años ingresó con náuseas, vómitos y dolor abdominal asociados con pérdida de peso (13 Kg en 3 meses). La endoscopia digestiva objetivó extensas alteraciones infiltrativas del cuerpo gástrico y los datos histopatológicos confirmaron el diagnóstico de adenocarcinoma gástrico Borrmann IV. Exámenes complementarios fueran indicativos de cáncer gástrico con metástasis óseas, caracterizadas por imágenes osteoblásticas diseminadas de la cintilografía del esqueleto. Se le administró quimioterapia paliativa como primera opción. Se elevó CA 19-9, CEA y apareció fosfatasis alcalina en el suero un indicador útil del diagnóstico. La extensión y el tipo de metástasis óseas pueden ser imágenes de cintilografías del esqueleto. Este caso tiene como objetivo describir la presencia de metástasis osteoblásticas diseminadas que raramente son producidas por este tipo de tumor.

Os efeitos cardiovasculares de vasicina, um alcalóide isolado das folhas de Sida cordifolia L., foi avaliado neste trabalho. Em ratos não-anestesiados (n=6), vasicina (1, 2.5, 5 e 10 mg/kg; i.v., aleatoriamente) induziu hipotensão associada com uma intensa bradicardia. Ambas as respostas buscaram completamente abolidas após atropina (2 mg/Kg; i.v.) e atenuadas após hexamethonio (20 mg/Kg; i.v.). Em anéis de artéria mesentérica de rato isolada, vasicina (0.03, 0.1, 0.3, 1, 3, 10, 30, 100 e 300 g/mL, cumulativamente) induziu relaxamento concentração-dependente de tônus promovido por fenilefrina (IC50= 3.8 0.9 g/mL; n= 6). Em conclusão, os resultados mostram que vasicina produz hipotensão e bradicardia que parecem ser devidas à excitação de receptores muscarínicos cardíacos (direta e/ou indiretamente) e por uma diminuição das resistências periféricas.

The cardiovascular effects of vasicine, an alkaloid isolated from the leaves of Sida cordifolia L., were evaluated in this work. In non-anaesthetized rats (n=6), vasicine (1, 2.5, 5 and 10 mg/kg; i.v., randomly) induced hypotension associated with an intense bradycardia. Both responses were completely abolished after atropine (2mg/Kg; i.v.) and attenuated after hexamethonium (20 mg/Kg; i.v.). In isolated rat mesenteric artery rings, vasicine (0.03, 0.1, 0.3, 1, 3, 10, 30, 100 and 300 μg/mL, cumulatively) induced concentration-dependent relaxation of phenylephrine-induced tone (IC50= 3.8±0.9 μg/mL; n = 6). In conclusion, the results show that vasicine produce hypotension and bradycardia which appears to be due to the stimulation of cardiac muscarinic receptors (directly and/or indirectly), and by a decrease of the peripheral resistances.