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1.
Lipid Composition and Cytokines IL-10 and TGF-β1 Evaluation of Prebiotic Ice Cream Containing Human Milk
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Castro, Matheus C.
; Alves, Eloize S.
; Saqueti, Bruno Henrique F.
Ferreira, Cintia Stephany R.
Zacarias, Joana Maira V.
Visentainer, Jeane Eliete L.
Madrona, Grasiele S.
Santos, Oscar O.
Visentainer, Jesui V.


Journal of the Brazilian Chemical Society
- Métricas do periódico
Human milk (HM) is recognized as an ideal nutritional source to meet the developmental needs of babies. However, a significant portion does not meet quality standards for immediate consumption, rendering it an interesting raw material for the production of by-products. This study aimed to evaluate the lipid composition and cytokines interleukin-10 (IL-10) and transforming growth factor-beta 1 (TGF-β1) in ice cream containing HM. Three formulations were developed: F1 with 93.46% HM, F2 with 46.73% cows’ milk and 46.73% HM, and F3 with 46.73% infant formula and 46.73% HM. Fatty acids (FA) and their nutritional quality, the triacylglycerol (TAG) profile, and the concentrations of cytokines IL-10 and TGF-β1 were analyzed. F1 and F3 exhibited the highest concentrations of essential FAs (18:2n-6 and 18:3n-3) compared to F2. This trend extended to eicosapentaenoic acid + docosahexaenoic acid, with higher values in F1 (10.62 ± 0.26) and F3 (11.16 ± 0.28) versus F2 (3.67 ± 0.18). Both cytokines IL-10 and TGF-β1, present in the ice creams, experienced a decrease in concentration due to the processing of the raw materials. Therefore, ice creams produced with HM have promising potential, making them recommended for use, providing a new destination for waste, and creating a novel product that can be used as a complementary food.
2.
Direct Methylation Method for Quantification of Fatty Acids in Lyophilized Human Milk by Gas Chromatography with Flame Ionization Detector
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Souza, Patrícia M.
; Santos, Patrícia D. S.
Ponhozi, Isadora B.
Cruz, Victor Hugo M.
Pizzo, Jessica S.
Alves, Eloize S.
Santos, Oscar O.
Visentainer, Jesuí V.

Journal of the Brazilian Chemical Society
- Métricas do periódico
This study aims to propose a new method for derivatizing fatty acids (FAs) from human milk (HM), eliminating the lipid extraction step, and simplifying the preparation for gas chromatography with a flame ionization detection (GC-FID) analysis to quantify the FAs. The Design Expert software optimized the reaction times, concentrations, and sample amount. The proposed method (PM) was validated for lyophilized HM and results for the figures of merit for precision in relative standard deviation (RSD) (RSDintra-day 1.34-4.03% and RSDinter day 2.08 5.16%), accuracy (99.87-102.16%), and robustness are within a linear range of 3 to 38% lipids in HM samples. The atmospheric solids analysis probe tandem mass spectrometry (ASAP-MS/MS) technique confirmed the efficiency of PM by expressing the molecular composition of triacylglycerol formed by FAs from the GC-FID technique. The PM requires a small sample size and conducts derivatization directly in the sample matrix, minimizing extraction errors. (FAs HM, , (HM) step GCFID GC FID (GC-FID times concentrations amount (PM RSD (RSD RSDintraday RSDintra 1.344.03% 134403 1.34 4.03% 1 34 4 03 1.34-4.03 208 2 08 2.0 5.16%, 516 5.16% 5 16 5.16%) 99.87102.16%, 998710216 99.87 102.16% 99 87 102 (99.87-102.16%) 38 samples ASAPMS/MS ASAPMSMS ASAP MS/MS MS (ASAP-MS/MS matrix errors (HM 344 1.344.03 13440 134 1.3 403 4.03 0 1.34-4.0 20 2. 51 5.16 87102 99.87102.16% 99871021 9987 99.8 10216 102.16 9 8 10 (99.87-102.16% ASAPMS MSMS 1.344.0 1344 13 1. 40 4.0 1.34-4. 5.1 8710 99.87102.16 9987102 998 99. 1021 102.1 (99.87-102.16 1.344. 4. 1.34-4 5. 871 99.87102.1 998710 102. (99.87-102.1 1.344 1.34- 99.87102. 99871 (99.87-102. 99.87102 (99.87-102 99.8710 (99.87-10 99.871 (99.87-1 (99.87- (99.87 (99.8 (99. (99 (9 (
3.
Ultrasound-Assisted Extraction of Sunflower Seed Oil Enriched with Active Compounds from Jambolan Leaf UltrasoundAssisted Ultrasound Assisted
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Journal of the Brazilian Chemical Society
- Métricas do periódico
This work aimed to incorporate the active compounds of the jambolan leaf in sunflower seed oil, in order to increase the nutritional quality of the oil. For this purpose, ultrasound-assisted extraction was conducted to establish the process conditions related to temperature, potency, solvent:sample ratio and time to obtain the enriched sunflower seed oil (ESSO). An experimental design was applied to examine the effect of variables (temperature, power, solvent:sample ratio and time) on the extraction mass yield (EMY). The application of maximum temperature, increased power, sample:solvent ratio and time resulted in higher EMY. Maximum theoretical EMY value was 35.16% (60 °C, 100%, 1:12 g mL-1 and 15 min). The characterization of the oils obtained from ESSO and sunflower seed oil (SSO) verified a higher content of flavonoids, phenolic compounds, a large proportion of active compounds, antioxidant activity, content of phytosterols, α-tocopherol and squalene, and induction time for ESSO. purpose ultrasoundassisted ultrasound assisted temperature potency solventsample solvent sample . (ESSO) (temperature power (EMY) samplesolvent 3516 35 16 35.16 60 (6 C °C 100 100% 112 1 12 1:1 mL1 mL mL- min. min min) SSO (SSO flavonoids activity phytosterols αtocopherol α tocopherol squalene (ESSO (EMY 351 3 35.1 6 ( 10 11 1: 35.
4.
Evaluation of the Lipid Composition of Commercially Available Evening Primrose Products by Gas Chromatography and Mass Spectrometry
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Figueiredo, Alisson L.
; Santos, Patricia D. S.
Fernandes, Cleverton S.
Fernandes, Ingrid L.
Assakawa, Amanda C.
Basso, Ernani A.
Santos, Oscar O.

Journal of the Brazilian Chemical Society
- Métricas do periódico
Evening primrose oil supplements are rich in omega-6 fatty acids, with emphasis on linoleic and γ-linolenic acids, being taken in the treatment and prevention of diseases, but mainly for the relief of symptoms caused by premenstrual tension and menopause in women. This study aimed to evaluate the quality of the lipid profile of evening primrose oil supplements using gas chromatography techniques with a flame ionization detector and mass spectrometry with electrospray ionization. For this, eight commercially available national evening primrose oil brands were used. Of the eight samples analyzed, only two met all the standards established in legislation for fatty acids. The remaining capsules were subjected to triacylglycerol analysis and using a calibration curve the presence of soybean oil in the composition of the evening primrose oil capsules was verified. From the triacylglycerol with a mass/charge ratio of 901, it was possible to identify the addition of 31.16-85.88% of soybean oil in six of the eight capsules, leading the consumer to ingest products that did not comply with current legislation. omega6 omega 6 omega- acids γlinolenic γ linolenic diseases women this used analyzed verified masscharge charge 901 31.1685.88% 31168588 31.16 85.88% 31 16 85 88 31.16-85.88 90 1685 31.1685.88 3116858 3116 31.1 8588 85.88 3 1 8 31.16-85.8 9 168 31.1685.8 311685 311 31. 858 85.8 31.16-85. 31.1685. 31168 85. 31.16-85 31.1685 31.16-8 31.168 31.16-
5.
COMPARATIVE ANALYSIS OF THE FATTY ACID COMPOSITION IN HUMAN, COW AND GOAT MILK AND THEIR INTERPOSITIONAL DISTRIBUTION IN TRIACYLGLYCEROLS HUMAN
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Ferreira, Giovanna C. A.
; Santos, Patricia D.
Zangirolami, Marcela S.
Figueiredo, Alisson L.
Fernandes, Cleverton S.
Silva, Geovane A. R. da
Basso, Ernani A.
Santos, Oscar O.

Exploring the triacylglycerols composition in milk samples from different ruminant species is crucial for dairy product development. Identifying the fatty acid (FA) composition in human milk is essential and is usually performed using gas chromatography (GC). Heat maps are useful for visualizing these results, aiding in pattern identification and relationships between samples and variables. Additionally, nuclear magnetic resonance (NMR) spectroscopy provides important qualitative and quantitative information about fatty acids (FA). In the present study, we compare the fatty acid composition in samples of HM, cow milk, and goat milk, as well as to demonstrate their positional distribution in triacylglycerols (TAGs). Human milk contains 49.21% saturated fatty acids (SFAs), 34.32% monounsaturated fatty acids (MUFA), and 16.47% polyunsaturated fatty acids (PUFA), including 2.14% docosahexaenoic acid (DHA) and 13.02% linoleic acid (LA). On the other hand, cow and goat milk have higher proportions of SFAs (56.2 and 58.02%, respectively) and MUFAs (40.56 and 40.95%, respectively), but lower amounts of PUFAs (3.24 and 1.03%, respectively), with significantly lower quantities of DHA (0.03 and 0.43%, respectively) and LA (1.46 and 2.76%, respectively). Additionally, fatty acids in ruminant milk mainly consist of long-chain fatty acids (LCFAs), with oleic acid being the most abundant. development FA (FA GC. GC . (GC) results variables Additionally NMR (NMR FA. study HM TAGs. TAGs (TAGs) 4921 49 21 49.21 SFAs, , (SFAs) 3432 34 32 34.32 MUFA, MUFA (MUFA) 1647 16 47 16.47 PUFA, PUFA (PUFA) 214 2 14 2.14 (DHA 1302 13 02 13.02 LA. (LA) hand 56.2 562 56 (56. 5802 58 58.02% respectively 40.56 4056 40 (40.5 4095 95 40.95% respectively, 3.24 324 3 24 (3.2 103 1 03 1.03% 0.03 003 0 (0.0 043 43 0.43% 1.46 146 46 (1.4 276 76 2.76% respectively. longchain long chain LCFAs, LCFAs (LCFAs) abundant (GC (TAGs 492 4 49.2 (SFAs 343 34.3 (MUFA 164 16.4 (PUFA 2.1 130 13.0 (LA 56. 5 (56 580 58.02 40.5 405 (40. 409 9 40.95 3.2 (3. 10 1.03 0.0 00 (0. 04 0.43 1.4 (1. 27 7 2.76 (LCFAs 49. 34. 16. 2. 13. (5 58.0 40. (40 40.9 3. (3 1.0 0. (0 0.4 1. (1 2.7 ( 58. (4
6.
Impact of Conservation Processes on the Lipid Profile and Immunological Factors IL-10 and TGF-β1 in Whey Separated from Discarded Human Milk
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Alves, Eloize S.
; Castro, Matheus C.
; Saqueti, Bruno H. F.
; Manin, Luciana P.
Alencar, Josiane B.
Zacarias, Joana M. V.
Bruni, Andressa R. S.
Madrona, Grasiele S.
Visentainer, Jeane E. L.
Cristianini, Marcelo
Santos, Oscar O.
Visentainer, Jesui V.



Journal of the Brazilian Chemical Society
- Métricas do periódico
Reuse made by the separation of whey can reduce the waste of human milk. However, the authors are not aware of the literature on treatments in human whey, made available by pasteurization holder, freeze-drying, spray drying, and high hydrostatic pressure. In this context, the present study applied treatments to human whey and evaluated their lipid and immunological quality. Among the results, a different formation in the triacylglycerol profile was evidenced after the application of spray drying and high hydrostatic pressure, while pasteurization and freeze-drying presented ion intensity close to the control human whey. In addition, pasteurization proved to be adequate for lipid nutritional quality and transformation factor-β1 (TGF-β1) concentration, while an increase in interleukin-10 (IL-10) levels was promoted, between 73 and 80%, after freeze-drying, spray drying, and high hydrostatic pressure. Through the principal component analysis, it is noteworthy that the processes presented divergences in terms of the effects caused, with similarity only between pasteurization and freeze-drying in the composition of fatty acids. However, it was observed that all processes were able to maintain the nutrients. Nevertheless, it is relevant to consider individual characteristics presented and the interest in the desired quality, which can be promising as a complementary product to infant feeding.
7.
Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil Brasil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
Slipinski, Adam
Linzmeier, Adelita M.
Calor, Adolfo R.
Garda, Adrian A.
Kury, Adriano B.
Fernandes, Agatha C.S.
Agudo-Padrón, Aisur I.
Akama, Alberto
Silva Neto, Alberto M. da
Burbano, Alejandro L.
Menezes, Aleksandra
Pereira-Colavite, Alessandre
Anichtchenko, Alexander
Lees, Alexander C.
Bezerra, Alexandra M.R.
Domahovski, Alexandre C.
Pimenta, Alexandre D.
Aleixo, Alexandre L.P.
Marceniuk, Alexandre P.
Paula, Alexandre S. de
Somavilla, Alexandre
Specht, Alexandre
Camargo, Alexssandro
Newton, Alfred F.
Silva, Aline A.S. da
Santos, Aline B. dos
Tassi, Aline D.
Aragão, Allan C.
Santos, Allan P.M.
Migotto, Alvaro E.
Mendes, Amanda C.
Cunha, Amanda
Chagas Júnior, Amazonas
Sousa, Ana A.T. de
Pavan, Ana C.
Almeida, Ana C.S.
Peronti, Ana L.B.G.
Henriques-Oliveira, Ana L.
Prudente, Ana L.
Tourinho, Ana L.
Pes, Ana M.O.
Carmignotto, Ana P.
Wengrat, Ana P.G. da Silva
Dornellas, Ana P.S.
Molin, Anamaria Dal
Puker, Anderson
Morandini, André C.
Ferreira, André da S.
Martins, André L.
Esteves, André M.
Fernandes, André S.
Roza, André S.
Köhler, Andreas
Paladini, Andressa
Andrade, Andrey J. de
Pinto, Ângelo P.
Salles, Anna C. de A.
Gondim, Anne I.
Amaral, Antonia C.Z.
Rondón, Antonio A.A.
Brescovit, Antonio
Lofego, Antônio C.
Marques, Antonio C.
Macedo, Antonio
Andriolo, Artur
Henriques, Augusto L.
Ferreira Júnior, Augusto L.
Lima, Aurino F. de
Barros, Ávyla R. de A.
Brito, Ayrton do R.
Romera, Bárbara L.V.
Vasconcelos, Beatriz M.C. de
Frable, Benjamin W.
Santos, Bernardo F.
Ferraz, Bernardo R.
Rosa, Brunno B.
Sampaio, Brunno H.L.
Bellini, Bruno C.
Clarkson, Bruno
Oliveira, Bruno G. de
Corrêa, Caio C.D.
Martins, Caleb C.
Castro-Guedes, Camila F. de
Souto, Camilla
Bicho, Carla de L.
Cunha, Carlo M.
Barboza, Carlos A. de M.
Lucena, Carlos A.S. de
Barreto, Carlos
Santana, Carlos D.C.M. de
Agne, Carlos E.Q.
Mielke, Carlos G.C.
Caetano, Carlos H.S.
Flechtmann, Carlos H.W.
Lamas, Carlos J.E.
Rocha, Carlos
Mascarenhas, Carolina S.
Margaría, Cecilia B.
Waichert, Cecilia
Digiani, Celina
Haddad, Célio F.B.
Azevedo, Celso O.
Benetti, Cesar J.
Santos, Charles M.D. dos
Bartlett, Charles R.
Bonvicino, Cibele
Ribeiro-Costa, Cibele S.
Santos, Cinthya S.G.
Justino, Cíntia E.L.
Canedo, Clarissa
Bonecker, Claudia C.
Santos, Cláudia P.
Carvalho, Claudio J.B. de
Gonçalves, Clayton C.
Galvão, Cleber
Costa, Cleide
Oliveira, Cléo D.C. de
Schwertner, Cristiano F.
Andrade, Cristiano L.
Pereira, Cristiano M.
Sampaio, Cristiano
Dias, Cristina de O.
Lucena, Daercio A. de A.
Manfio, Daiara
Amorim, Dalton de S.
Queiroz, Dalva L. de
Queiroz, Dalva L. de
Colpani, Daniara
Abbate, Daniel
Aquino, Daniel A.
Burckhardt, Daniel
Cavallari, Daniel C.
Prado, Daniel de C. Schelesky
Praciano, Daniel L.
Basílio, Daniel S.
Bená, Daniela de C.
Toledo, Daniela G.P. de
Takiya, Daniela M.
Fernandes, Daniell R.R.
Ament, Danilo C.
Cordeiro, Danilo P.
Silva, Darliane E.
Pollock, Darren A.
Muniz, David B.
Gibson, David I.
Nogueira, David S.
Marques, Dayse W.A.
Lucatelli, Débora
Garcia, Deivys M.A.
Baêta, Délio
Ferreira, Denise N.M.
Rueda-Ramírez, Diana
Fachin, Diego A.
Souza, Diego de S.
Rodrigues, Diego F.
Pádua, Diego G. de
Barbosa, Diego N.
Dolibaina, Diego R.
Amaral, Diogo C.
Chandler, Donald S.
Maccagnan, Douglas H.B.
Caron, Edilson
Carvalho, Edrielly
Adriano, Edson A.
Abreu Júnior, Edson F. de
Pereira, Edson H.L.
Viegas, Eduarda F.G.
Carneiro, Eduardo
Colley, Eduardo
Eizirik, Eduardo
Santos, Eduardo F. dos
Shimbori, Eduardo M.
Suárez-Morales, Eduardo
Arruda, Eliane P. de
Chiquito, Elisandra A.
Lima, Élison F.B.
Castro, Elizeu B. de
Orlandin, Elton
Nascimento, Elynton A. do
Razzolini, Emanuel
Gama, Emanuel R.R.
Araujo, Enilma M. de
Nishiyama, Eric Y.
Spiessberger, Erich L.
Santos, Érika C.L. dos
Contreras, Eugenia F.
Galati, Eunice A.B.
Oliveira Junior, Evaldo C. de
Gallardo, Fabiana
Hernandes, Fabio A.
Lansac-Tôha, Fábio A.
Pitombo, Fabio B.
Dario, Fabio Di
Santos, Fábio L. dos
Mauro, Fabio
Nascimento, Fabio O. do
Olmos, Fabio
Amaral, Fabio R.
Schunck, Fabio
Godoi, Fábio S. P. de
Machado, Fabrizio M.
Barbo, Fausto E.
Agrain, Federico A.
Ribeiro, Felipe B.
Moreira, Felipe F.F.
Barbosa, Felipe F.
Silva, Fenanda S.
Cavalcanti, Fernanda F.
Straube, Fernando C.
Carbayo, Fernando
Carvalho Filho, Fernando
Zanella, Fernando C.V.
Jacinavicius, Fernando de C.
Farache, Fernando H.A.
Leivas, Fernando
Dias, Fernando M.S.
Mantellato, Fernando
Vaz-de-Mello, Fernando Z.
Gudin, Filipe M.
Albuquerque, Flávio
Molina, Flavio B.
Passos, Flávio D.
Shockley, Floyd W.
Pinheiro, Francielly F.
Mello, Francisco de A.G. de
Nascimento, Francisco E. de L.
Franco, Francisco L.
Oliveira, Francisco L. de
Melo, Francisco T. de V.
Quijano, Freddy R.B.
Salles, Frederico F.
Biffi, Gabriel
Queiroz, Gabriel C.
Bizarro, Gabriel L.
Hrycyna, Gabriela
Leviski, Gabriela
Powell, Gareth S.
Santos, Geane B. dos
Morse, Geoffrey E.
Brown, George
Mattox, George M.T.
Zimbrão, Geraldo
Carvalho, Gervásio S.
Miranda, Gil F.G.
Moraes, Gilberto J. de
Lourido, Gilcélia M.
Neves, Gilmar P.
Moreira, Gilson R.P.
Montingelli, Giovanna G.
Maurício, Giovanni N.
Marconato, Gláucia
Lopez, Guilherme E.L.
Silva, Guilherme L. da
Muricy, Guilherme
Brito, Guilherme R.R.
Garbino, Guilherme S.T.
Flores, Gustavo E.
Graciolli, Gustavo
Libardi, Gustavo S.
Proctor, Heather C.
Gil-Santana, Helcio R.
Varella, Henrique R.
Escalona, Hermes E.
Schmitz, Hermes J.
Rodrigues, Higor D.D.
Galvão Filho, Hilton de C.
Quintino, Hingrid Y.S.
Pinto, Hudson A.
Rainho, Hugo L.
Miyahira, Igor C.
Gonçalves, Igor de S.
Martins, Inês X.
Cardoso, Irene A.
Oliveira, Ismael B. de
Franz, Ismael
Fernandes, Itanna O.
Golfetti, Ivan F.
S. Campos-Filho, Ivanklin
Oliveira, Ivo de S.
Delabie, Jacques H.C.
Oliveira, Jader de
Prando, Jadila S.
Patton, James L.
Bitencourt, Jamille de A.
Silva, Janaina M.
Santos, Jandir C.
Arruda, Janine O.
Valderrama, Jefferson S.
Dalapicolla, Jeronymo
Oliveira, Jéssica P.
Hájek, Jiri
Morselli, João P.
Narita, João P.
Martin, João P.I.
Grazia, Jocélia
McHugh, Joe
Cherem, Jorge J.
Farias Júnior, José A.S.
Fernandes, Jose A.M.
Pacheco, José F.
Birindelli, José L.O.
Rezende, José M.
Avendaño, Jose M.
Duarte, José M. Barbanti
Ribeiro, José R. Inácio
Mermudes, José R.M.
Pujol-Luz, José R.
Santos, Josenilson R. dos
Câmara, Josenir T.
Teixeira, Joyce A.
Prado, Joyce R. do
Botero, Juan P.
Almeida, Julia C.
Kohler, Julia
Gonçalves, Julia P.
Beneti, Julia S.
Donahue, Julian P.
Alvim, Juliana
Almeida, Juliana C.
Segadilha, Juliana L.
Wingert, Juliana M.
Barbosa, Julianna F.
Ferrer, Juliano
Santos, Juliano F. dos
Kuabara, Kamila M.D.
Nascimento, Karine B.
Schoeninger, Karine
Campião, Karla M.
Soares, Karla
Zilch, Kássia
Barão, Kim R.
Teixeira, Larissa
Sousa, Laura D. do N.M. de
Dumas, Leandro L.
Vieira, Leandro M.
Azevedo, Leonardo H.G.
Carvalho, Leonardo S.
Souza, Leonardo S. de
Rocha, Leonardo S.G.
Bernardi, Leopoldo F.O.
Vieira, Letícia M.
Johann, Liana
Salvatierra, Lidianne
Oliveira, Livia de M.
Loureiro, Lourdes M.A. El-moor
Barreto, Luana B.
Barros, Luana M.
Lecci, Lucas
Camargos, Lucas M. de
Lima, Lucas R.C.
Almeida, Lucia M.
Martins, Luciana R.
Marinoni, Luciane
Moura, Luciano de A.
Lima, Luciano
Naka, Luciano N.
Miranda, Lucília S.
Salik, Lucy M.
Bezerra, Luis E.A.
Silveira, Luis F.
Campos, Luiz A.
Castro, Luiz A.S. de
Pinho, Luiz C.
Silveira, Luiz F.L.
Iniesta, Luiz F.M.
Tencatt, Luiz F.C.
Simone, Luiz R.L.
Malabarba, Luiz R.
Cruz, Luiza S. da
Sekerka, Lukas
Barros, Lurdiana D.
Santos, Luziany Q.
Skoracki, Maciej
Correia, Maira A.
Uchoa, Manoel A.
Andrade, Manuella F.G.
Hermes, Marcel G.
Miranda, Marcel S.
Araújo, Marcel S. de
Monné, Marcela L.
Labruna, Marcelo B.
Santis, Marcelo D. de
Duarte, Marcelo
Knoff, Marcelo
Nogueira, Marcelo
Britto, Marcelo R. de
Melo, Marcelo R.S. de
Carvalho, Marcelo R. de
Tavares, Marcelo T.
Kitahara, Marcelo V.
Justo, Marcia C.N.
Botelho, Marcia J.C.
Couri, Márcia S.
Borges-Martins, Márcio
Felix, Márcio
Oliveira, Marcio L. de
Bologna, Marco A.
Gottschalk, Marco S.
Tavares, Marcos D.S.
Lhano, Marcos G.
Bevilaqua, Marcus
Santos, Marcus T.T.
Domingues, Marcus V.
Sallum, Maria A.M.
Digiani, María C.
Santarém, Maria C.A.
Nascimento, Maria C. do
Becerril, María de los A.M.
Santos, Maria E.A. dos
Passos, Maria I. da S. dos
Felippe-Bauer, Maria L.
Cherman, Mariana A.
Terossi, Mariana
Bartz, Marie L.C.
Barbosa, Marina F. de C.
Loeb, Marina V.
Cohn-Haft, Mario
Cupello, Mario
Martins, Marlúcia B.
Christofersen, Martin L.
Bento, Matheus
Rocha, Matheus dos S.
Martins, Maurício L.
Segura, Melissa O.
Cardenas, Melissa Q.
Duarte, Mércia E.
Ivie, Michael A.
Mincarone, Michael M.
Borges, Michela
Monné, Miguel A.
Casagrande, Mirna M.
Fernandez, Monica A.
Piovesan, Mônica
Menezes, Naércio A.
Benaim, Natalia P.
Reategui, Natália S.
Pedro, Natan C.
Pecly, Nathalia H.
Ferreira Júnior, Nelson
Silva Júnior, Nelson J. da
Perioto, Nelson W.
Hamada, Neusa
Degallier, Nicolas
Chao, Ning L.
Ferla, Noeli J.
Mielke, Olaf H.H.
Evangelista, Olivia
Shibatta, Oscar A.
Oliveira, Otto M.P.
Albornoz, Pablo C.L.
Dellapé, Pablo M.
Gonçalves, Pablo R.
Shimabukuro, Paloma H.F.
Grossi, Paschoal
Rodrigues, Patrícia E. da S.
Lima, Patricia O.V.
Velazco, Paul
Santos, Paula B. dos
Araújo, Paula B.
Silva, Paula K.R.
Riccardi, Paula R.
Garcia, Paulo C. de A.
Passos, Paulo G.H.
Corgosinho, Paulo H.C.
Lucinda, Paulo
Costa, Paulo M.S.
Alves, Paulo P.
Roth, Paulo R. de O.
Coelho, Paulo R.S.
Duarte, Paulo R.M.
Carvalho, Pedro F. de
Gnaspini, Pedro
Souza-Dias, Pedro G.B.
Linardi, Pedro M.
Bartholomay, Pedro R.
Demite, Peterson R.
Bulirsch, Petr
Boll, Piter K.
Pereira, Rachel M.M.
Silva, Rafael A.P.F.
Moura, Rafael B. de
Boldrini, Rafael
Silva, Rafaela A. da
Falaschi, Rafaela L.
Cordeiro, Ralf T.S.
Mello, Ramon J.C.L.
Singer, Randal A.
Querino, Ranyse B.
Heleodoro, Raphael A.
Castilho, Raphael de C.
Constantino, Reginaldo
Guedes, Reinaldo C.
Carrenho, Renan
Gomes, Renata S.
Gregorin, Renato
Machado, Renato J.P.
Bérnils, Renato S.
Capellari, Renato S.
Silva, Ricardo B.
Kawada, Ricardo
Dias, Ricardo M.
Siewert, Ricardo
Brugnera, Ricaro
Leschen, Richard A.B.
Constantin, Robert
Robbins, Robert
Pinto, Roberta R.
Reis, Roberto E. dos
Ramos, Robson T. da C.
Cavichioli, Rodney R.
Barros, Rodolfo C. de
Caires, Rodrigo A.
Salvador, Rodrigo B.
Marques, Rodrigo C.
Araújo, Rodrigo C.
Araujo, Rodrigo de O.
Dios, Rodrigo de V.P.
Johnsson, Rodrigo
Feitosa, Rodrigo M.
Hutchings, Roger W.
Lara, Rogéria I.R.
Rossi, Rogério V.
Gerstmeier, Roland
Ochoa, Ronald
Hutchings, Rosa S.G.
Ale-Rocha, Rosaly
Rocha, Rosana M. da
Tidon, Rosana
Brito, Rosangela
Pellens, Roseli
Santos, Sabrina R. dos
Santos, Sandra D. dos
Paiva, Sandra V.
Santos, Sandro
Oliveira, Sarah S. de
Costa, Sávio C.
Gardner, Scott L.
Leal, Sebastián A. Muñoz
Aloquio, Sergio
Bonecker, Sergio L.C.
Bueno, Sergio L. de S.
Almeida, Sérgio M. de
Stampar, Sérgio N.
Andena, Sérgio R.
Posso, Sergio R.
Lima, Sheila P.
Gadelha, Sian de S.
Thiengo, Silvana C.
Cohen, Simone C.
Brandão, Simone N.
Rosa, Simone P.
Ribeiro, Síria L.B.
Letana, Sócrates D.
Santos, Sonia B. dos
Andrade, Sonia C.S.
Dávila, Stephane
Vaz, Stéphanie
Peck, Stewart B.
Christo, Susete W.
Cunha, Suzan B.Z.
Gomes, Suzete R.
Duarte, Tácio
Madeira-Ott, Taís
Marques, Taísa
Roell, Talita
Lima, Tarcilla C. de
Sepulveda, Tatiana A.
Maria, Tatiana F.
Ruschel, Tatiana P.
Rodrigues, Thaiana
Marinho, Thais A.
Almeida, Thaís M. de
Miranda, Thaís P.
Freitas, Thales R.O.
Pereira, Thalles P.L.
Zacca, Thamara
Pacheco, Thaynara L.
Martins, Thiago F.
Alvarenga, Thiago M.
Carvalho, Thiago R. de
Polizei, Thiago T.S.
McElrath, Thomas C.
Henry, Thomas
Pikart, Tiago G.
Porto, Tiago J.
Krolow, Tiago K.
Carvalho, Tiago P.
Lotufo, Tito M. da C.
Caramaschi, Ulisses
Pinheiro, Ulisses dos S.
Pardiñas, Ulyses F.J.
Maia, Valéria C.
Tavares, Valeria
Costa, Valmir A.
Amaral, Vanessa S. do
Silva, Vera C.
Wolff, Vera R. dos S.
Slobodian, Verônica
Silva, Vinícius B. da
Espíndola, Vinicius C.
Costa-Silva, Vinicius da
Bertaco, Vinicius de A.
Padula, Vinícius
Ferreira, Vinicius S.
Silva, Vitor C.P. da
Piacentini, Vítor de Q.
Sandoval-Gómez, Vivian E.
Trevine, Vivian
Sousa, Viviane R.
Sant’Anna, Vivianne B. de
Mathis, Wayne N.
Souza, Wesley de O.
Colombo, Wesley D.
Tomaszewska, Wioletta
Wosiacki, Wolmar B.
Ovando, Ximena M.C.
Leite, Yuri L.R.








ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others. publications problem uptodate up date classifications context exception (CTFB http//fauna.jbrj.gov.br/, httpfaunajbrjgovbr http //fauna.jbrj.gov.br/ , jbrj gov br (http://fauna.jbrj.gov.br/) 2015 Brazil 80 specialists 1 2024 133691 133 691 133,69 125138 125 138 125,13 82.3%, 823 82 3 (82.3% 102000 102 000 102,00 7.69%, 769 7 69 (7.69% 11000 11 11,00 . 3,567 3567 567 (3,56 2,292 2292 2 292 (2,29 1,833 1833 833 (1,83 1,447 1447 447 (1,44 1000 1,00 831 (83 628 (62 606 (60 520 (52 50 users science health biology law anthropology education others http//fauna.jbrj.gov.br/ faunajbrjgovbr //fauna.jbrj.gov.br (http://fauna.jbrj.gov.br/ 201 8 202 13369 13 133,6 12513 12 125,1 82.3% (82.3 10200 10 00 102,0 7.69% 76 6 (7.69 1100 11,0 3,56 356 56 (3,5 2,29 229 29 (2,2 1,83 183 83 (1,8 1,44 144 44 (1,4 100 1,0 (8 62 (6 60 52 (5 5 http//fauna.jbrj.gov.br (http://fauna.jbrj.gov.br 20 1336 133, 1251 125, 82.3 (82. 1020 0 102, 7.69 (7.6 110 11, 3,5 35 (3, 2,2 22 (2, 1,8 18 (1, 1,4 14 4 ( 82. (82 7.6 (7. 3, (3 2, (2 (1 7. (7
8.
Study of the Stability and Homogeneity of Powdered Egg Matrix as a Candidate for Certified Reference Material for the Determination of Centesimal Composition
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Lopes, Tiago Antonio M.
; Chiavelli, Lucas Ulisses R.
Santos, Patrícia Daniele S. dos
Visentainer, Jesuí V.
Santos, Oscar O.

Journal of the Brazilian Chemical Society
- Métricas do periódico
The full process of fresh egg preservation presents limitations and is associated with high transportation costs, while processed powdered eggs offer numerous advantages, such as high stability against degradation, providing extended shelf life, easy production, transportation, and favorable storage conditions. Traditional methods for determining the proximate composition of eggs and derivatives lack the capability to eliminate the matrix effect and do not guarantee adequate precision of the results. Therefore, Certified Reference Material (CRM) are the most suitable and effective tool to achieve this purpose. However, in Brazil, CRMs for powdered egg matrix are currently unavailable, although it is not possible to eliminate matrix effects, these can be included in the measurement uncertainty of each CRM. This study aimed to develop a candidate CRM for the powdered egg matrix with the requirements for the competence of testing and calibration laboratories (17034:2016) for proximate composition analyses. The analysis of variance (ANOVA) results for stability and homogeneity indicated no significant variation among the data obtained in the tests and the storage time of the material. Consequently, it was concluded that the powdered egg matrix is stable under simulated transportation conditions (short-term stability) as well as storage conditions (long-term stability) and exhibits adequate homogeneity for a CRM candidate. costs advantages degradation life production Therefore (CRM purpose However Brazil unavailable effects 170342016 17034 2016 (17034:2016 analyses ANOVA (ANOVA material Consequently shortterm short term longterm long 17034201 1703 201 (17034:201 1703420 170 20 (17034:20 170342 17 2 (17034:2 1 (17034: (17034 (1703 (170 (17 (1 (
9.
Antioxidant Potential Optimization of Mixed Vegetable Juice: A Chemometric Approach Juice
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Journal of the Brazilian Chemical Society
- Métricas do periódico
This study aimed to develop a healthy and antioxidant-rich mixed juice. The effect of the vegetables combination on the juice characteristics was evaluated and, to optimize the proportion components of the mixture, chemometric modeling was used. The maximum antioxidant activity was considered as a variable response. The mixed juice was evaluated for technological parameters (content of soluble solids, titratable acidity, density, viscosity, sedimentation, and color parameters). The phenolic compounds present in the vegetables were identified by ultra-high performance liquid chromatography-mass spectometry (UHPLC-MS) technique. The special cubic model was considered significant (p < 0.05), with low dispersion and homogeneous antioxidant activity data (coefficient of variation (C.V.) = 3.73%) and the most appropriate for statistical data analysis (R2 = 0.9937). The optimal formulation consisted of a blend (m/m) with orange (73.37%), apple (20.45%), kale (5.58%), and ginger (0.59%) with greater desirability, 12534.2 μmol mL-1 of validated antioxidant activity and adequate physicochemical characteristics. The addition of orange juice resulted in a product with better functionality, due to the increased antioxidant capacity. This innovative study resulted in a functional formulation that involves the combination of low-cost vegetables with the maximum possible antioxidant action, capable of helping to protect against damage caused by free radicals. antioxidantrich rich mixture used response content solids acidity density viscosity sedimentation parameters. . parameters) ultrahigh ultra high chromatographymass chromatography mass UHPLCMS UHPLC MS (UHPLC-MS technique p 0.05, 005 0.05 , 0 05 0.05) coefficient C.V. CV C V (C.V. 3.73% 373 3 73 R2 R (R 0.9937. 09937 0.9937 9937 0.9937) m/m mm m (m/m 73.37%, 7337 73.37% 37 (73.37%) 20.45%, 2045 20.45% 20 45 (20.45%) 5.58%, 558 5.58% 5 58 (5.58%) 0.59% 059 59 (0.59% desirability 125342 12534 2 12534. mL1 mL 1 mL- functionality capacity lowcost cost action radicals 00 0.0 C.V (C.V 3.73 7 0993 0.993 993 733 73.37 (73.37% 204 20.45 4 (20.45% 55 5.58 (5.58% 0.59 (0.59 1253 0. 3.7 099 0.99 99 73.3 (73.37 20.4 (20.45 5.5 (5.58 0.5 (0.5 125 3. 09 0.9 9 73. (73.3 20. (20.4 5. (5.5 (0. 12 (73. (20. (5. (0 (73 (20 (5 ( (7 (2
10.
Evaluation of Lipid Composition and Nutritional Quality of Olive Oil Varieties Using ESI-MS, GC-FID and Chemometrics Techniques ESIMS, ESIMS ESI MS, MS ESI-MS GCFID GC FID
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Figueiredo, Alisson L.
; Silva, Michele C.
; Pizzo, Jessica S.
; Santos, Patrícia D. S.
; Manin, Luciana P.
Leôncio, Mariana S.
Visentainer, Jesuí V.
Santos, Oscar O.




Journal of the Brazilian Chemical Society
- Métricas do periódico
Olive oil is a highly demanded product renowned for its unique taste and diverse nutrient content, primarily composed of lipids. This study aimed to comprehensively analyze the lipid profiles of four olive varieties (Arbequina, Arbosana, Koroneiki, and Frantoio) and their corresponding commercial olive oils using advanced analytical techniques, namely electrospray ionization mass spectrometry and gas chromatography with flame ionization detector, combined with principal component analysis. The nutritional quality of the olive oils was evaluated based on parameters such as the ratio of saturated acids to polyunsaturated acids, atherogenicity, thrombogenicity, and andhypocholesterolemic/hypercholesterolemic indexes. The study revealed that the primary fatty acids identified were palmitic, stearic, oleic, and linoleic acids, while triolein, palmitodiolein, and stearodiolein emerged as the most abundant triacylglycerol across all samples. Furthermore, the results demonstrated that the commercial olive oil samples exhibited an exceptional nutritional profile. Overall, this study highlights the significance and efficacy of the analytical and statistical techniques employed to unravel the lipid profiles of olive oils, a product of substantial commercial value and susceptible to adulteration. Additionally, it emphasizes the importance of utilizing nutritional quality indices to assess the health benefits associated with olive oil consumption. content lipids Arbequina, Arbequina (Arbequina Arbosana Koroneiki Frantoio detector analysis atherogenicity thrombogenicity andhypocholesterolemichypercholesterolemic andhypocholesterolemic hypercholesterolemic indexes palmitic stearic oleic triolein palmitodiolein Furthermore profile Overall adulteration Additionally consumption
11.
CHEMICAL CONSTITUENTS AND ANTIBACTERIAL ACTIVITY OF THREE TYPES OF AMAZONIAN Melipona spp. GEOPROPOLIS spp
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Santos, Darlan C. dos
; David, Jorge M.
; Neto, Oscar C. S.
; Lima, Brenda O.
Yatsuda, Regiane
Moreira, Bruno O.
Marques, Lucas M.
Frazão, Richardson F.



This work describes the first chemical study on the metabolic profile of three geopropolis samples from Melipona fulva, M. compressipes, and M. paraensis collected in the Amazonian region. The samples were submitted to solvent extractions. The silylated hexane extracts were submitted to GC-MS analysis and tentatively identified mainly the presence of triterpenes, steroids, fatty acids, and alcohols. The chemical profile of M. fulva of and M. compressipes geopropolis are similar. However, cholesterol, triacontanol, and palmitic acid are the main compounds in M. fulva; in M. compressipes, palmitic acid, linoleic acid, and oleic acid are predominant. The chemical profile of the M. paraensis sample is entirely different once more than 60% of the extract comprises lupenone and triacontanol. The CHCl3 soluble fraction of the MeOH extract of the M. paraensis geopropolis was submitted to chromatographic techniques, and it afforded cycloartenol, 24-methylene-cycloartenol, lupeol, α- and β-amirins, and 7-O-methylaromadendrin. The MeOH extract of this sample presents antimicrobial activities against strains of Streptococcus mutans, S. sobrinus, and S. aureus (MIC of 15.6-62.5 µg mL−1 and MBC of 62.5-1000 µg mL−1). The M. fulva and M. compressipes geopropolis were not active in this test. M region extractions GCMS GC MS triterpenes steroids acids alcohols similar However cholesterol triacontanol predominant 60 CHCl techniques cycloartenol 24methylenecycloartenol, 24methylenecycloartenol methylenecycloartenol 24 methylene 24-methylene-cycloartenol lupeol α βamirins, βamirins β amirins, amirins β-amirins 7Omethylaromadendrin. 7Omethylaromadendrin Omethylaromadendrin 7 O methylaromadendrin. methylaromadendrin 7-O-methylaromadendrin mutans S sobrinus MIC 15.662.5 156625 15.6 62.5 15 6 62 5 15.6-62. mL1 mL 1 mL− 62.51000 6251000 1000 62.5-100 mL−1. . mL−1) test 2 662 15.662. 15662 156 15. 625 62. 15.6-62 51000 62.5100 625100 100 62.5-10 66 15.662 1566 15.6-6 5100 62.510 62510 10 62.5-1 15.66 15.6- 510 62.51 6251 62.5- 51
12.
Validation of an Analytical Method by Headspace Gas Chromatography with Flame Ionization and Evaluation of Matrix Effect of Volatile Compounds in Cat Food
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Carvalho, Bruno M.
; Santos, Patrícia D. S. dos
; Silva, Geovane A. R. da
; Senes, Carlos E. R.
Visentainer, Jesuí V.
Santos, Oscar O.



Journal of the Brazilian Chemical Society
- Métricas do periódico
This study describes the validation of a headspace gas chromatography with flame ionization (HS-GC-FID) method for the determination of propanal, pentanal, hexanal, octanal, and trans-2-nonenal originated by lipid oxidation from cat food samples, as well as the evaluation of the matrix effect in the results. This method was applied to the analysis of commercial cat food and proved to be suitable for the determination of these volatile compounds in different samples. Mean recoveries between 88 and 109% were obtained and repeatability expressed as relative standard deviation was always lower than 6.95%. The intra- and inter-assay precisions ranged from 0.44 to 20.88% and from 0.45 to 20.52%, respectively. In addition, the matrix effect of cat food samples was determined by comparing the slopes of the standard addition method, and the external calibration curve and its influences were verified. These results highlight the high potential of this method, which allows the determination of lipid oxidation products in cat food samples directly, without requiring prior sample preparation techniques.
13.
Lipid Profile of Human Milk in Different Lactation Stages Submitted to Pasteurization, Lyophilization and Spray-Drying Processes Pasteurization SprayDrying Spray Drying
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Neia, Vanessa J. C.
; Santos, Patrícia D. S.
; Tavares, Christyna B. G.
; Paula, Meliana G.
; Costa, Silvio C.
; Zacarias, Joana M. V.
; Alencar, Josiane B.
; Silveira, Roberta
Santos, Oscar O.
Visentainer, Jeane E. L.
Visentainer, Jesuí V.







Journal of the Brazilian Chemical Society
- Métricas do periódico
The objective of this study was to evaluate the effect of pasteurization, lyophilization and spray-drying on the lipid profile of donated human milk (DHM) at different lactation stages. Nine frozen samples of colostrum, transitional and raw mature human milk (HM) were collected from the Human Milk Bank (HMB) of the Hospital Universitário de Maringá (HUM). Samples were thawed and pooled according to lactation stage. Thereafter, it was submitted to pasteurization, lyophilization and spray-drying processes and later the fatty acid (FA) composition and the lipid profile analyses were performed. Gas chromatograph (GC) with flame ionization detector (FID) and electrospray ionization (ESI) mass spectrometer (MS) were used, respectively. In the FA composition analysis, it was observed that the same classes of saturated FA (SFA), monounsaturated FA (MUFA) and polyunsaturated FA (PUFA) were preserved throughout the process and in all lactation stages. The lipid profiles were also preserved after processing. Therefore, the lyophilization and spray-drying processes are promising techniques to preserve the DHM in the HMB, once the components evaluated were preserved, and both techniques facilitate the transport and storage, as the techniques reduce the sample volume. pasteurization spraydrying spray drying (DHM stages colostrum HM (HM HMB (HMB HUM. HUM . (HUM) stage Thereafter (FA performed GC (GC FID (FID ESI (ESI MS (MS used respectively analysis SFA, SFA , (SFA) MUFA (MUFA PUFA (PUFA processing Therefore storage volume (HUM (SFA
14.
Effects of Pasteurization and Freeze Drying on Proximal Composition, Fatty Acids Composition and Lipid Quality in Colostrum Human Milk
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Ientz, Gislaine A. S.
; Alves, Eloize S.
; Castro, Matheus C.
Frigo, Giovana
Tavares, Christyna B. G.
Visentainer, Jeane E. L.
Santos, Oscar O.
Visentainer, Jesui V.


Journal of the Brazilian Chemical Society
- Métricas do periódico
Colostrum human milk samples obtained from human milk banks received different treatments and were evaluated for their proximal composition, fatty acids composition and lipid quality. The total lipids content was maintained in the treatments. There was no difference in moisture, ash, total proteins, carbohydrates and energetic value for raw colostrum human milk and pasteurized colostrum human milk, and there was difference in ash, total proteins and energetic value for freeze-dried colostrum human milk and pasteurized + freeze-dried colostrum human milk. The fatty acids composition demonstrated no difference in the processes for 14 of 31 fatty acids identified. Furthermore, lipid quality indices were obtained, which were unpublished for colostrum human milk submitted to pasteurization and freeze drying. Principal component analysis showed similar behaviors for freeze-dried colostrum human milk and pasteurized and freeze-dried colostrum human milk. So, the processes used may be promising for application in colostrum human milk, due to the maintenance of its proximal characteristics and fatty acid composition.
15.
Determination of Ethyl Carbamate in Commercial Sweetened Sugar Cane Spirit by ESI-MS/MS Using Modified QuEChERS and 18-Crown-6/Trifluoroacetic Acid Spiking Additives
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Tonin, Angélica P. P.
; Poliseli, Camila B.
; Sinosaki, Nayane M.
; Martinez, Fernanda C.
Santos, Oscar O.
Oliveira, Cláudio C.
Silva, Valquiria M.
Visentainer, Jesuí V.
Meurer, Eduardo C.



Journal of the Brazilian Chemical Society
- Métricas do periódico
A fast, sensitive, and selective direct injection electrospray tandem mass spectrometry (DI ESI MS/MS) method that is able to quantify ethyl carbamate in commercial sweetened sugar cane spirit is described. The preparation method uses a modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) with potassium carbonate added to sweetened sugar cane spirit to separate the aqueous phase from the ethanol phase. The aqueous phase contains sucrose that suppresses electrospray ionization. Ethyl carbamate supernatant from the ethanol phase is transferred and enriched with 18-crown-6/trifluoroacetic acid additives. The additives sequester metal cations reducing the ionization of sodium and potassium, favoring the detection of ethyl carbamate as sole protonated cations. The method was successfully applied for the quantification of eleven real samples and certified sugar cane spirit demonstrating its applicability for quality control and regulatory analysis. The method showed reliable analytical parameters compared to conventional gas chromatography mass spectrometry (GC-MS) method commonly used for ethyl carbamate analysis. DI-ESI-MS/MS method requires just a fast step sample clean up and presents consistent values for the limit of detection (LOD 48.0 μg L-1) and limit of quantification (LOQ 160.0 μg L-1). Furthermore, the recoveries obtained were close to 100%, with relative standard deviations below 10% of sample certificates.
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