In silico and in vitro tests can reveal promising anti-leishmania activity for natural products and their derivatives. The aim of this study was to investigate in silico the pharmacological activities of potential new chalcones and their leishmanicidal potential in vitro. The in silico study was carried out using the PASS, MolPredictX and Molegro Virtual Docker 6.0 programs. Antiparasitic activity was assessed in axenic promastigote and amastigote forms of Leishmania braziliensis. The cytotoxicity tests used the J77G8 cell line. The chalcones exhibited 50% cytotoxic concentration values (CC50) values > 50 μM. Chalcone 4 (named FERAI) presented the best activity with concentration for 50% of promastigotes and intracellular parasites forms (EC50) of 9.75 ± 1.7 and 10.13 ± 1.7 μM for promastigote and amastigote, respectively. Reactive oxygen species (ROS) testing presented increased ROS levels in the parasite at the FERAI concentrations of 10 μM (56.33%), 20 μM (61.76%) and 30 μM (67.13%). Molecular docking revealed interactions (binding energy) between FERAI and the enzymes UDP-glycosyl pyrophosphorylase (-56.8384), dihydroorotate-dehydrogenase (-132.276) and trypanothione-reductase (-151.281). Our results demonstrated the anti-leishmanial activity of chalcones, especially FERAI, with a noted raising of ROS levels in the parasite. Molecular docking revealed dihydroorotate dehydrogenase and trypanothione reductase as potential pharmacological targets for FERAI. antileishmania anti leishmania derivatives PASS 60 6 0 6. programs braziliensis JG J G J77G line CC50 CC (CC50 5 named EC50 EC (EC50 975 9 75 9.7 17 1 7 1. 1013 13 10.1 respectively (ROS 56.33%, 5633 56.33% , 56 33 (56.33%) 2 61.76% 6176 61 76 (61.76% 3 67.13%. 6713 67.13% . 67 (67.13%) binding energy UDPglycosyl UDP glycosyl 56.8384, 568384 56.8384 8384 (-56.8384) dihydroorotatedehydrogenase 132.276 132276 132 276 (-132.276 trypanothionereductase 151.281. 151281 151.281 151 281 (-151.281) antileishmanial leishmanial CC5 (CC5 EC5 (EC5 97 9. 101 10. 563 56.33 (56.33% 61.76 617 (61.76 671 67.13 (67.13% 56838 56.838 838 (-56.8384 132.27 13227 27 (-132.27 15128 151.28 15 28 (-151.281 (CC (EC 56.3 (56.33 61.7 (61.7 67.1 (67.13 5683 56.83 83 (-56.838 132.2 1322 (-132.2 1512 151.2 (-151.28 56. (56.3 61. (61. 67. (67.1 568 56.8 8 (-56.83 132. (-132. 151. (-151.2 (56. (61 (67. (-56.8 (-132 (-151. (56 (6 (67 (-56. (-13 (-151 (5 ( (-56 (-1 (-15 (-5 (-

ABSTRACT: Surface contamination by pathogenic and deteriorating microorganisms is a constant concern in the food industry. Therefore, in this study we evaluated the antimicrobial activity of silver nanoparticles (AgNPs), produced using the essential oil of Lippia origanoides Kunth and ethanolic extract of Hymenaea martiana Hayne, against bacteria used in milk processing. For this, AgNPs were synthesized and their antimicrobial activity was evaluated for minimum inhibitory and bactericidal concentrations, time-kill, interference on the biofilm, and application on the surfaces of different materials. AgNPs showed bacteriostatic and bactericidal activity against Staphylococcus aureus (ATCC 25923), Staphylococcus aureus (ATCC 33591), Escherichia coli (ATCC 25922), Salmonella enterica subsp. enterica serovar Choleraesuis (ATCC 10708), Listeria monocytogenes, Escherichia coli, Salmonella spp.,andPseudomonas aeruginosa,and interfered with biofilm formation more than with consolidated biofilms. A 1hour period was sufficient to reduce the bacterial cells, whereas a Minimum Duration for Killing (MDK) of 99% was reached after approximately 30 min. AgNPs were effective against bacteria attached to stainless steel and polyethylene, but ineffective on tile surfaces. Thus, owing to the growing microbial resistance and the need to develop new products based on the concepts of green chemistry, these AgNPs are presented as a new possibility for cleaning processes in the food industry. ABSTRACT industry Therefore AgNPs, , (AgNPs) Hayne processing concentrations timekill, timekill time kill, kill time-kill materials ATCC 25923, 25923 25923) 33591, 33591 33591) 25922, 25922 25922) subsp 10708, 10708 10708) monocytogenes sppandPseudomonas spp andPseudomonas aeruginosaand aeruginosa biofilms hour cells MDK (MDK 99 3 min polyethylene Thus chemistry (AgNPs 2592 3359 1070 9 259 335 107 25 33 10 2 1

RESUMO: A contaminação de superfícies por micro-organismos patogênicos e deteriorantes é uma preocupação constante na indústria de alimentos. Dessa forma, o objetivo deste trabalho foi avaliar a atividade antimicrobiana de nanopartículas de prata (AgNPs) produzidas utilizando óleo essencial de Lippia origanoides Kunth e extrato etanólico da Hymenaea martiana Hayne, frente a bactérias de importância na contaminação em áreas de processamento do leite. Para isso, as AgNPs foram sintetizadas e sua atividade antimicrobiana foi avaliada através das concentrações inibitória e bactericida mínimas, tempo de morte, interferência sobre o biofilme, e por fim, a sua aplicação sobre superfícies de diferentes materiais. As AgNPs apresentaram atividade bacteriostática e/ou bactericida frente à Staphylococcus aureus (ATCC 25923), Staphylococcus aureus (ATCC 33591), Escherichia coli (ATCC 25922), Salmonella enterica subsp. enterica serovar Choleraesuis (ATCC 10708), Listeria monocytogenes, Escherichia coli, Salmonella spp. e Pseudomonas aeruginosa, e interferiram na formação do biofilme mais do que sobre o biofilme consolidado. O tempo de uma hora foi o suficiente para reduzir as populações bacterianas, enquanto o Minimum Duration for Killing MDK 99% foi atingido próximo a 30 minutos. As AgNPs foram eficientes contra bactérias aderidas ao inox e ao polietileno. Assim, tendo em vista a crescente resistência microbiana e o desenvolvimento de novos produtos baseados nos conceitos da química verde, essas nanopartículas de prata apresentam-se como uma nova possibilidade de uso nos processos de higienização da indústria de alimentos. RESUMO microorganismos micro organismos alimentos forma (AgNPs Hayne leite isso mínimas morte fim materiais eou ou ATCC 25923, 25923 , 25923) 33591, 33591 33591) 25922, 25922 25922) subsp 10708, 10708 10708) monocytogenes spp aeruginosa consolidado bacterianas 99 3 minutos polietileno Assim verde apresentamse apresentam se 2592 3359 1070 9 259 335 107 25 33 10 2 1

ABSTRACT BACKGROUND: The prevalence of chronic kidney disease (CKD) has increased in the recent decades, along with the number of patients in the terminal stages of this disease, requiring transplantation. Some skin disorders are more frequent in patients with CKD and in renal transplant recipients (RTR). OBJECTIVES: To evaluate the frequency of skin diseases in RTR and patients with CKD receiving conservative treatment. DESIGN AND SETTING: This observational cross-sectional study recruited consecutive patients with CKD and RTR from a nephrology clinic at a teaching hospital in Brazil between 2015 and 2020. METHODS: Quantitative, descriptive, and analytical approaches were used. The sample was selected based on convenience sampling. Data were collected from dermatological visits and participants’ medical records. RESULTS: Overall, 308 participants were included: 206 RTR (66.9%, median age: 48 years, interquartile range [IQR] 38.0–56.0, 63.6% men) and 102 patients with CKD (33.1%, median age: 61.0 years, IQR 50.0–71.2, 48% men). The frequency of infectious skin diseases (39.3% vs. 21.6% P = 0.002) were higher in RTR than in patients with CKD. Neoplastic skin lesions were present in nine (4.4%) RTR and in only one (1.0%) patient with CKD. Among the RTR, the ratio of basal cell carcinoma to squamous cell carcinoma was 2:1. CONCLUSIONS: This study revealed that an increased frequency of infectious skin diseases may be expected in patients who have undergone kidney transplantation. Among skin cancers, BCC is more frequently observed in RTR, especially in those using azathioprine. BACKGROUND (CKD decades transplantation RTR. . (RTR) OBJECTIVES treatment SETTING crosssectional cross sectional 201 2020 METHODS Quantitative descriptive used sampling records RESULTS Overall 30 included 20 66.9%, 669 66 9 (66.9% age 4 years [IQR 380560 38 0 56 38.0–56.0 636 63 6 63.6 men 10 33.1%, 331 33 1 (33.1% 610 61 61. 500712 50 71 2 50.0–71.2 men. 39.3% 393 39 3 (39.3 vs 216 21 21.6 0.002 0002 002 4.4% 44 (4.4% 1.0% (1.0% 21. 1. 2:1 CONCLUSIONS cancers azathioprine (RTR 202 66.9% (66.9 38056 5 38.0–56. 63. 33.1% (33.1 50071 7 50.0–71. 39.3 (39. 0.00 000 00 4.4 (4.4 1.0 (1.0 2: 66.9 (66. 3805 38.0–56 33.1 (33. 5007 50.0–71 39. (39 0.0 4. (4. (1. 66. (66 380 38.0–5 33. (33 500 50.0–7 (3 0. (4 (1 (6 38.0– 50.0– ( 38.0 50.0 38. 50.

Abstract The presence of land use mix (LUM) in a neighborhood has been shown as an important aspect to promote walking as a daily means of transport. However, few studies in the area have applied different measurement strategies to test their effect on alternative modes of travel behavior, such as older adults walking. We applied six LUM measures of land use mix in two neighborhood definitions (500 and 1000m network buffers) and assessed their associations with walking for transport outcomes in adults over age 60 years from the EpiFloripa Aging study, living in the municipality of Florianópolis, Brazil, in 2013/14. Accounting for sociodemographic and environmental variables, adjusted associations found a positive relationship between four LUM and walking. The entropy index and three alternative measures defined by the intensity of commercial and nonresidential uses were positively associated with the walking for transportation, regardless of neighborhood definition. Stronger positive associations were seen when using smaller buffers and measures of the proportion of commercial uses, proportion of nonresidential uses and destination density. The results show that alternative measurements can overcome the entropy index, pointing out the need to adapt LUM measures and neighborhood scale to the geographic context and age group under analysis. (LUM However behavior 500 (50 m 6 study Florianópolis Brazil 201314 2013 14 2013/14 variables transportation definition density analysis 50 (5 20131 201 1 2013/1 5 ( 20 2013/ 2

Resumo A presença de diferentes usos do solo em um bairro tem se mostrado um aspecto importante para promover a caminhada como meio de transporte diário. No entanto, poucos estudos na área aplicaram diferentes estratégias de mensuração para testar seu efeito em modos alternativos de comportamento de viagem, como a caminhada de idosos. Aplicamos seis medidas de uso do solo em duas definições de bairro (buffers de rede de 500 e 1000m) e avaliamos suas associações com a caminhada para fins de transporte em participantes (≥ 60 anos) do estudo EpiFloripa Idoso (2013/14) de Florianópolis, Brasil. Considerando variáveis sociodemográficas e ambientais, as associações ajustadas encontraram uma relação positiva entre quatro medidas e a caminhada. O índice de entropia e três medidas alternativas de intensidade de usos não residenciais associaram-se positivamente com a caminhada para transporte, independentemente da definição de bairro. Associações positivas mais fortes foram observadas ao usar buffers menores e medidas de proporção de usos comerciais, proporção de usos não residenciais e densidade de destinos. Os resultados mostram que medidas alternativas podem superar o índice de entropia, apontando a necessidade de adaptação das métricas e da escala de vizinhança ao contexto geográfico e à faixa etária em análise. diário entanto viagem idosos 50 1000m m ≥ ( 6 anos 2013/14 201314 2013 14 (2013/14 Florianópolis Brasil ambientais associaramse associaram comerciais destinos análise 5 2013/1 20131 201 1 (2013/1 2013/ 20 (2013/ 2 (2013 (201 (20 (2

ABSTRACT The varied clinical presentations of SARS-CoV-2 infection have raised concerns about long-term consequences, especially “long-COVID” or “post-COVID-19 syndrome.” In this context, the cardiorespiratory optimal point (COP) within the Cardiopulmonary Exercise Test (CPET) emerges as a crucial metric for evaluating functional capacities and detecting cardiovascular and pulmonary anomalies post-COVID-19. This study aimed to assess COP values among post-COVID-19 patients and categorized them based on the initial severity of their disease. In this cross-sectional study conducted in the Northeast Brazil, 80 patients (26 females and 54 males) previously infected with SARS-CoV-2 underwent CPET. We clinically stratified patients into mild, moderate, or severe COVID-19 categories and assessed COP values and other cardiorespiratory metrics. We found differences in the predicted COP between patients with mild and severe COVID-19 (p=0.042). Additionally, patients with moderate and severe COVID-19 record had an average COP value exceeding 22. Other parameters, including respiratory exchange ratio, heart rate, and oxygen uptake efficiency slope, did not differ across the groups. Patients with a history of severe COVID-19 showed altered COP values, suggesting potential discrepancies in cardiovascular and respiratory system integration. The outcomes emphasize the importance of continuous monitoring and assessment of the cardiorespiratory domain for post-COVID-19 patients. Further research is needed to understand the relationship between elevated COP in post-severe COVID-19 and its long-term prognostic implications. SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- longterm long term consequences longCOVID COVID “long-COVID postCOVID19 postCOVID post 19 “post-COVID-1 syndrome. syndrome context (COP CPET (CPET postCOVID19. 19. post-COVID-1 disease crosssectional cross sectional Brazil 8 26 (2 5 males COVID19 COVID-1 metrics p=0.042. p0042 p p=0.042 . 0 042 (p=0.042) Additionally 22 parameters ratio rate slope groups integration postsevere implications SARS-CoV postCOVID1 1 “post-COVID- post-COVID- ( COVID1 COVID- p004 p=0.04 04 (p=0.042 “post-COVID post-COVID p00 p=0.0 (p=0.04 p0 p=0. (p=0.0 p=0 (p=0. p= (p=0 (p= (p

ABSTRACT Objective Currently programmed cell death protein 1 (PD-1) inhibitors in combination with other therapies are being evaluated to determine their efficacy in cancer treatment. However, the effect of PD-ligand (L) 1 expression on disease outcomes in stage III (EC III) non-small cell lung cancer is not completely understood. Therefore, this study aimed to assess the influence of PD-L1 expression on the outcomes of EC III non-small cell lung cancer. Methods This study was conducted on patients diagnosed with EC III non-small cell lung cancer who underwent treatment at a tertiary care hospital. PD-L1 expression was determined using immunohistochemical staining, all patients expressed PD-L1. Survival was estimated using the Kaplan-Meier method. Relationships between variables were assessed using Cox proportional regression models. Results A total of 49 patients (median age=69 years) with EC III non-small cell lung cancer and PD-L1 expression were evaluated. More than half of the patients were men, and most were regular smokers. The patients were treated with neoadjuvant chemotherapy, surgery, or sequential or combined chemotherapy and radiotherapy. The median progression-free survival of the entire cohort was 14.2 months, and the median overall survival was 20 months. There was no significant association between PD-L1 expression and disease progression, clinical characteristics, or overall survival. Conclusions PD-L1 expression was not correlated with EC III non-small cell lung cancer outcomes. Whether these findings differ from the association with immune checkpoint inhibitors remains to be addressed in future studies. PD1 PD (PD-1 However PDligand ligand L (L nonsmall non small understood Therefore PDL1 PDL L1 PD-L hospital staining PDL1. L1. KaplanMeier Kaplan Meier method models 4 age69 age 69 age=6 years men smokers surgery radiotherapy progressionfree progression free 142 14 2 14. months characteristics studies (PD- age6 6 age= (PD

El uso de herramientas de la salud digital se ha intensificado en la Atención Primaria de la Salud (APS) y en las prácticas de Agentes Comunitarias de Salud (ACS). El objetivo de este artículo es analizar los desafíos impuestos por las condiciones de trabajo de las ACS en el contexto de la salud digital. Se trata de una investigación cualitativa, con triangulación de métodos, envolviendo liderazgos sindicales, gestores y profesionales de la salud. Los resultados señalan una reedición de viejos desafíos alrededor del trabajo de las ACS, tales como mayor burocratización, control, división social y técnica. Sin embargo, surgen nuevos desafíos alrededor del mantenimiento, calidad de los instrumentos y formación profesional. Se concluye demarcando la necesidad de una garantía logística, financiera y política para la implementación de la salud digital en el trabajo de las ACS. APS (APS . (ACS) cualitativa métodos sindicales burocratización control técnica embargo mantenimiento profesional logística (ACS

The use of digital health tools has grown in intensity in Primary Health Care (PHC) and in the practices of Community Health Workers (CHWs). This article aims to analyze the challenges imposed by the working conditions of CHWs in the context of digital health. It is a qualitative study, with triangulation of methods involving union leaders, managers and health professionals. The results indicate the re-emergence of old challenges surrounding the work of CHWs, such as greater bureaucratization, control, social and technical division of work. However, new challenges emerge around maintenance, quality of tools and professional training. In conclusion, there is a need for logistical, financial and political safeguards for the implementation of digital health in the work of CHWs. PHC (PHC . (CHWs) study leaders professionals reemergence re emergence bureaucratization control However maintenance training conclusion logistical (CHWs

O uso de ferramentas da saúde digital tem sido intensificado na Atenção Primária à Saúde (APS) e nas práticas de agentes comunitários de saúde (ACS). Este artigo tem como objetivo analisar os desafios impostos pelas condições de trabalho dos ACS no contexto da saúde digital. Trata-se de uma pesquisa qualitativa com triangulação de métodos envolvendo lideranças sindicais, gestores e profissionais da saúde. Os resultados apontam uma reedição de velhos desafios em torno do trabalho dos ACS, como maior burocratização, controle, divisão social e técnica. Porém, novos desafios emergem em torno da manutenção, da qualidade dos instrumentos e da formação profissional. Conclui-se demarcando a necessidade de uma garantia logística, financeira e política para a implementação da saúde digital no trabalho dos ACS. APS (APS . (ACS) Tratase Trata se sindicais burocratização controle técnica Porém manutenção profissional Concluise Conclui logística (ACS

ABSTRACT Post-acute COVID-19 syndrome, or long COVID, presents with persistent symptoms, including cough, dyspnea, and fatigue, extending beyond one month after SARS-CoV-2 infection. Cardiac complications such as chest pain and arrhythmias have raised concerns, with chronotropic incompetence (CI), an inadequate heart rate increase during exercise, emerging as a significant condition contributing to diminished exercise tolerance and quality of life. This study estimated the prevalence of CI and explored its association with aerobic capacity and physical activity levels in long COVID patients. A cross-sectional study was conducted at a private hospital in Sergipe, Brazil, involving 93 patients over 18 years old with persistent post-COVID-19 symptoms after confirmed SARS-CoV-2 infections. Exclusion criteria included beta-blocker use, inadequate respiratory exchange ratio, and inability to complete cardiopulmonary exercise testing (CPET). Clinical histories, CPET results, and chronotropic index calculation were used to identify CI, with logistic regression analyzing associated factors. Of the participants (mean age 45 years; average duration since COVID-19 diagnosis 120 days), 20.4% were diagnosed with CI. Logistic regression identified a strong association between CI and sedentary behavior (OR 11.80; 95% CI 2.54 to 54.78; p=0.001). Patients with CI showed lower predicted peak heart rates and maximal oxygen uptake. The prevalence of CI among long COVID patients in this study was approximately 20%, associated with decreased aerobic capacity and increased sedentary behavior. These findings highlight the need for timely diagnosis and therapeutic interventions, including cardiopulmonary rehabilitation, to enhance the quality of life in post-COVID patients with CI. The study’s cross-sectional design and its specific context have limited causality inference and generalizability, underscoring the importance of further research in diverse settings. Postacute Post acute COVID19 19 COVID-1 syndrome cough dyspnea fatigue SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- infection concerns , (CI) crosssectional cross sectional Sergipe Brazil 9 1 postCOVID19 postCOVID post post-COVID-1 infections betablocker beta blocker use ratio CPET. . (CPET) histories results factors mean 4 12 days, days days) 204 20 20.4 OR 11.80 1180 11 80 95 254 54 2.5 54.78 5478 78 p=0.001. p0001 p p=0.001 0 001 p=0.001) uptake 20% interventions rehabilitation studys s generalizability settings COVID1 COVID- SARS-CoV (CI postCOVID1 post-COVID- (CPET 20. 11.8 118 8 25 5 2. 54.7 547 7 p000 p=0.00 00 11. 54. p00 p=0.0 p0 p=0. p=0 p=

ABSTRACT: Native to the tropical Americas, guava (Psidium guajava L.) is an important crop in Brazil. However, the emergence of so-called guava decline, a complex disease resulting from root parasitism by the root-knot nematode (Meloidogyne enterolobii Yang & Eisenback) in association with opportunistic fungi, has decimated guava orchards across Brazil and in other countries. In the present study, seminiferous guava accessions were vegetatively propagated by minigrafting and their genotypes preserved for resistance reassessment in clones to confirm or not host plant reactions. The results indicated a highly virulent parasite, high host suitability of the P. guajava species, and widely varying reactions among plants of the same genotype and between different genotypes, demonstrating that the strategy of preserving the germplasm and reassessing reactions in clones may be important in identifying and selecting germplasms with a degree of resistance to M. enterolobii. The progeny of cv. Paluma P02R5R2 obtained the lowest average parasite reproduction factor (RF = 22.11) among the genotypes evaluated and was; therefore; classified as moderately resistant and preserved for future research. ABSTRACT Americas Psidium L. L However socalled so called decline rootknot knot Meloidogyne Eisenback fungi countries study P species M cv PRR R P02R5R RF 22.11 2211 22 11 was therefore research 22.1 221 2 1 22.

RESUMO: Originária da América Tropical, a goiabeira (Psidium guajava L.) tem grande relevância para o Brasil. Contudo, o surgimento do patossistema designado como declínio da goiabeira, problema fitossanitário provocado pelo parasitismo das raízes pelo nematoide-das-galhas (Meloidogyne enterolobii Yang & Eisenback) em associação com fungos oportunistas, dizimou muitos pomares em todas as regiões do Brasil e em outros países. No presente estudo, propagou-se vegetativamente, por miniestaquia, acessos de goiabeiras seminíferas mantendo seus genótipos preservados e reavaliando a resistência por meio dos clones de forma a comprovar ou não as reações das plantas hospedeiras. Os resultados apontam para alta virulência do parasita, bem como alta hospedabilidade da espécie P. guajava, além da existência de grande variação da reação entre plantas do mesmo genótipo e entre genótipos distintos, o que indica que a estratégia de preservação do germoplasma e a reavaliação da reação em clones pode ser importante na busca e seleção de germoplasma com algum grau de resistência a M. enterolobii. A progênie da cv. Paluma P02R5R2 obteve a menor média de Fator de Reprodução do parasita (FR = 22,11) entre os genótipos avaliados, sendo classificada como moderadamente resistente e preservada para estudos posteriores. RESUMO Tropical Psidium L. L Contudo nematoidedasgalhas nematoide galhas Meloidogyne Eisenback oportunistas países estudo propagouse propagou se vegetativamente miniestaquia hospedeiras P distintos M cv PRR R P02R5R FR 22,11 2211 22 11 avaliados posteriores 22,1 221 2 1 22,

RESUMO Fundamento: As evidências acerca das sequelas a longo prazo após internação por síndrome do desconforto respiratório agudo por COVID-19 ainda são escassas. Objetivo: Avaliar alterações nas funções pulmonar, cardíaca e renal e na qualidade de vida após internação por síndrome do desconforto respiratório agudo secundária à COVID-19. Métodos: Trata-se de estudo multicêntrico, caso-controle, incluindo 220 participantes. Os casos serão definidos como pacientes hospitalizados por síndrome do desconforto respiratório agudo devido à COVID-19. No Grupo Controle, serão selecionados indivíduos sem histórico de hospitalização nos últimos 12 meses ou sintomas a longo prazo de COVID-19. Todos os indivíduos serão submetidos à espirometria pulmonar com teste de difusão pelo monóxido de carbono, tomografia de tórax, ressonância magnética cardíaca e renal com gadolínio, ergoespirometria, creatinina sérica e urinária, proteínas totais e microalbuminúria urinária, além de questionários de qualidade de vida. Os casos serão avaliados 12 meses após a alta hospitalar e os controles, 90 dias após a inclusão no estudo. Para todas as análises estatísticas, será assumido como significativo o valor p < 0,05. Resultados: O desfecho primário do estudo foi definido com a capacidade de difusão pulmonar aferida para o monóxido de carbono a partir de 12 meses. Os demais parâmetros das funções pulmonar, cardíacas e renal e da qualidade de vida foram definidos como desfechos secundários. Conclusão: Este estudo visa determinar as sequelas a longo prazo nas funções pulmonar, cardíaca e renal e na qualidade de vida de pacientes internados por síndrome do desconforto respiratório agudo devido à COVID-19 na população brasileira. Fundamento COVID19 COVID 19 COVID-1 escassas Objetivo COVID19. 19. Métodos Tratase Trata se multicêntrico casocontrole, casocontrole caso controle, controle caso-controle 22 participantes Controle 1 tórax gadolínio ergoespirometria urinária controles 9 estatísticas 005 0 05 0,05 Resultados secundários Conclusão brasileira COVID1 COVID- 2 00 0,0 0,

ABSTRACT Rationale: Evidence about long-term sequelae after hospitalization for acute respiratory distress syndrome due to COVID-19 is still scarce. Purpose: To evaluate changes in pulmonary, cardiac, and renal function and in quality of life after hospitalization for acute respiratory distress syndrome secondary to COVID-19. Methods: This will be a multicenter case–control study of 220 participants. Eligible are patients who are hospitalized for acute respiratory distress syndrome due to COVID-19. In the control group, individuals with no history of hospitalization in the last 12 months or long-term symptoms of COVID-19 will be selected. All individuals will be subjected to pulmonary spirometry with a carbon monoxide diffusion test, chest tomography, cardiac and renal magnetic resonance imaging with gadolinium, ergospirometry, serum and urinary creatinine, total protein, and urinary microalbuminuria, in addition to quality-of-life questionnaires. Patients will be evaluated 12 months after hospital discharge, and controls will be evaluated within 90 days of inclusion in the study. For all the statistical analyses, p < 0.05 is the threshold for significance. Results: The primary outcome of the study will be the pulmonary diffusing capacity for carbon monoxide measured after 12 months. The other parameters of pulmonary, cardiac, and renal function and quality of life are secondary outcomes. Conclusion: This study aims to determine the long-term sequelae of pulmonary, cardiac, and renal function and the quality of life of patients hospitalized for acute respiratory distress syndrome due to COVID-19 in the Brazilian population. Rationale longterm long term COVID19 COVID 19 COVID-1 scarce Purpose COVID19. 19. Methods casecontrol case 22 participants group 1 selected test tomography gadolinium ergospirometry creatinine protein microalbuminuria qualityoflife questionnaires discharge 9 analyses 005 0 05 0.0 significance Results outcomes Conclusion population COVID1 COVID- 2 00 0.

RESUMO O município de Serrinha está inserido no Polígono das Secas e apresenta formações de Floresta que merecem ter sua flora investigada, principalmente em se tratando de briófitas, uma vez que o conhecimento sobre a composição das Briófitas no Domínio Caatinga ainda é subestimado. A Serra Barra do Vento é uma formação de Floresta submontana, e neste ambiente foram encontradas 71 espécies de briófitas. Frullania intumescens (Lehm. & Lindenb.) Lehm. & Lindenb, Chionoloma orthodontum (Müll.Hal.) M. Alonso et al. e Chionoloma schlimii (Müll.Hal.) M. Alonso et al. estão sendo citadas pela primeira vez para a região Nordeste. Enquanto Fissidens brevipes Besch. e Campylopus griseus (Horsch.) A. Jaeger. estão sendo citadas pela primeira vez para o Estado da Bahia. investigada briófitas subestimado submontana 7 Lehm (Lehm Lindenb. Lindenb Müll.Hal. MüllHal Müll Hal (Müll.Hal. M al Nordeste Besch Horsch. Horsch (Horsch. Jaeger Bahia Müll.Hal (Müll.Hal (Horsch

ABSTRACT The municipality of Serrinha is part of the Polígono das Secas and has some forest formations that deserve to have their flora investigated, considering that the knowledge about the composition of Bryophytes in the Caatinga Domain is underestimated. Serra da Barra do Vento is a Submontane Seasonal Forest formation and in this environment 71 species of bryophytes were found. Frullania intumescens (Lehm. & Lindenb.) Lehm. & Lindenb, Chionoloma orthodontum (Müll.Hal.) M. Alonso et al., and Chionoloma schlimii (Müll.Hal.) M. Alonso et al. are being cited for the first time for the Northeast region. And the species Fissidens brevipes Besch., and Campylopus griseus (Horsch.) A. Jaeger. are being cited for the first time for Bahia State. investigated underestimated 7 found Lehm (Lehm Lindenb. Lindenb Müll.Hal. MüllHal Müll Hal (Müll.Hal. M al region Besch Besch. Horsch. Horsch (Horsch. A Jaeger State Müll.Hal (Müll.Hal (Horsch

SUMMARY OBJECTIVE: The objective of this study was to assess the clinical and uterine cervix characteristics of patients displaying vaginal discharge with positive results for Mycoplasma sp. and/or Ureaplasma spp. METHODS: An analytical cross-sectional study involving women aged 18–45 years was conducted. Microbiological assessments included Ureaplasma and Mycoplasma cultures, as well as human papillomavirus hybrid capture using ecto and endocervix swabs. All tests were two-tailed, and significance was set at p<0.05. RESULTS: Among 324 women, Ureaplasma prevalence was 17.9%, and Mycoplasma prevalence was 3.1%. The Ureaplasma-positive group exhibited a higher frequency of urinary tract infections (39.1 vs. 19%, p=0.002) and human papillomavirus (39.7 vs. 12.8%, p≤0.001) compared with controls. The Mycoplasma-positive group showed a higher frequency of non-contraceptive use compared with controls (66.2 vs. 30.0%, p=0.036). Abnormal colposcopic findings were more prevalent in the Mycoplasma/Ureaplasma-positive group than in controls (positive: 65% vs. control: 35%, p=0.001). Pap smear findings did not differ between the groups. CONCLUSION: Ureaplasma spp. was associated with urinary tract infections and human papillomavirus, while the presence of Mycoplasma sp. was linked to reduced contraceptive use. When analyzing both pathogens together, a higher frequency of abnormal colposcopic findings was observed, with no difference in cytological findings in the positive group. OBJECTIVE sp andor or spp METHODS crosssectional cross sectional 1845 18 45 18–4 conducted cultures swabs twotailed, twotailed two tailed, tailed two-tailed p005 p 0 05 p<0.05 RESULTS 32 179 17 9 17.9% 31 3 1 3.1% Ureaplasmapositive 39.1 391 39 (39. vs 19 19% p=0.002 p0002 002 39.7 397 7 128 12 8 12.8% p≤0.001 p0001 001 Mycoplasmapositive noncontraceptive non 66.2 662 66 2 (66. 300 30 30.0% p=0.036. p0036 p=0.036 . 036 p=0.036) Mycoplasma/Ureaplasmapositive MycoplasmaUreaplasmapositive Mycoplasma/Ureaplasma (positive 65 control 35 35% p=0.001. p=0.001 p=0.001) groups CONCLUSION together observed 184 4 18– p00 p<0.0 17.9 3.1 39. (39 p=0.00 p000 00 12.8 p≤0.00 66. 6 (66 30.0 p003 p=0.03 03 MycoplasmaUreaplasma p0 p<0. 17. 3. (3 p=0.0 12. p≤0.0 (6 30. p<0 ( p=0. p≤0. p< p=0 p≤0 p= p≤

Abstract Background There is limited data available regarding the prevalence of intracranial arterial stenosis (ICAS) among acute ischemic stroke (AIS) patients in Brazil and Latin America. Objective The present study sought to investigate the frequency and predictors of ICAS among patients with AIS or transient ischemic attack (TIA) in a Brazilian center, with transcranial color-coded duplex sonography (TCCS) technique. Methods Consecutive AIS and TIA patients, admitted to an academic public comprehensive stroke center in Brazil from February to December 2014, evaluated by TCCS were prospectively selected. Vascular narrowings > 50% were considered as ICAS, based on ultrasound criteria previously defined in the literature. Results We assessed 170 consecutive patients with AIS or TIA, of whom 27 (15.9%) were excluded due to an inadequate transtemporal acoustic bone window. We confirmed ICAS in 55 patients (38.5%). The most common location was the proximal segment of the middle cerebral artery (28.2%), followed by the vertebral (15.4%), posterior cerebral (13.6%), terminal internal carotid (9.1%) and basilar (8.2%) arteries. On multivariate models adjusting for potential confounders, systolic blood pressure (OR: 1.03, 95%CI: 1.01–1.04; p = 0.008) was independently associated with ICAS. Conclusion We found significant ICAS in approximately ⅓ of patients admitted with symptoms of AIS or TIA in a public tertiary academic stroke center in Brazil. The TCCS is an accessible and noninvasive technique that can be used to investigate the presence of moderate and severe ICAS, especially in patients who cannot be exposed to more invasive exams, such as the use of intravenous contrast agents. (ICAS (AIS America (TIA colorcoded color coded (TCCS 2014 selected 50 literature 17 2 15.9% 159 15 9 (15.9% window 5 38.5%. 385 38.5% . 38 (38.5%) 28.2%, 282 28.2% , 28 (28.2%) 15.4%, 154 15.4% 4 (15.4%) 13.6%, 136 13.6% 13 6 (13.6%) 9.1% 91 1 (9.1% 8.2% 82 8 (8.2% arteries confounders OR (OR 103 03 1.03 95%CI 95CI CI 95 1.01–1.04 101104 01 04 0.008 0008 0 008 exams agents 201 15.9 (15.9 38.5 3 (38.5% 28.2 (28.2% 15.4 (15.4% 13.6 (13.6% 9.1 (9.1 8.2 (8.2 10 1.0 1.01–1.0 10110 0.00 000 00 20 15. (15. 38. (38.5 28. (28.2 (15.4 13. (13.6 9. (9. 8. (8. 1. 1.01–1. 1011 0.0 (15 (38. (28. (13. (9 (8 1.01–1 101 0. (1 (38 (28 (13 ( 1.01– (3 (2 1.01

Resumo Antecedentes Dados acerca da prevalência da estenose arterial intracraniana (EAIC) entre os pacientes com acidente vascular isquêmico (AVCi) agudo no Brasil e América Latina são limitados. Objetivo O presente estudo pretendeu investigar a frequência e os preditores da EAIC nos pacientes AVCi ou ataque isquêmico transitório (AIT) em um centro brasileiro utilizando o Doppler transcraniano colorido (duplex transcraniano). Métodos Pacientes consecutivos com AVCi ou AIT, admitidos entre fevereiro e dezembro de 2014 em um centro acadêmico brasileiro especializado em doenças cerebrovasculares, foram avaliados prospectivamente com duplex transcraniano. Os estreitamentos vasculares > 50% foram considerados como EAIC, baseado em critérios ultrassonográficos definidos previamente na literatura. Resultados Foram avaliados 170 pacientes com AVCi ou AIT, dos quais 27 (15,9%) foram excluídos em decorrência da janela óssea transtemporal acústica inadequada. Confirmamos EAIC em 55 pacientes (38,5%). A localização mais comum foi o segmento proximal da artéria cerebral média (28,2%), seguida pelas artérias vertebral (15,4%), cerebral posterior (13,6%), carótida interna terminal (9,1%) e basilar (8,2%). No modelo multivariado, ajustado para os potenciais confundidores, a pressão arterial sistólica aumentada (OR: 1,03; IC 95%: 1,01–1,04; p = 0,008) foi independentemente associada a EAIC. Conclusão Foi identificada EAIC significativa em quase ⅓ dos pacientes admitidos com sintomas de AVCi ou AIT em um serviço acadêmico público de atendimento especializado em doenças cerebrovasculares. O Doppler transcraniano colorido é uma ferramenta acessível e não invasiva que pode ser utilizada com segurança para a investigação da presença de EAIC moderada ou grave, especialmente nos pacientes que não podem ser expostos a exames complementares mais invasivos com uso de contraste intravenoso. (EAIC (AVCi limitados (AIT . transcraniano) 201 cerebrovasculares 50 literatura 17 2 15,9% 159 15 9 (15,9% inadequada 5 38,5%. 385 38,5% 38 (38,5%) 28,2%, 282 28,2% , 28 (28,2%) 15,4%, 154 15,4% 4 (15,4%) 13,6%, 136 13,6% 13 6 (13,6%) 9,1% 91 1 (9,1% 8,2%. 82 8,2% 8 (8,2%) multivariado confundidores OR (OR 1,03 103 03 95% 95 1,01–1,04 101104 01 04 0,008 0008 0 008 grave intravenoso 20 15,9 (15,9 38,5 3 (38,5% 28,2 (28,2% 15,4 (15,4% 13,6 (13,6% 9,1 (9,1 8,2 (8,2% 1,0 10 1,01–1,0 10110 0,00 000 00 15, (15, 38, (38,5 28, (28,2 (15,4 13, (13,6 9, (9, 8, (8,2 1, 1,01–1, 1011 0,0 (15 (38, (28, (13, (9 (8, 1,01–1 101 0, (1 (38 (28 (13 ( (8 1,01– (3 (2 1,01