ABSTRACT Insects are fundamental to biodiversity conservation in almost all ecosystems, and their population decline, and extinction directly result from environmental impacts. These facts are aggravated by the lack of knowledge of insect biodiversity, the so-called biodiversity deficits, especially the Linnean and Wallacean shortfalls. In freshwater ecosystems, biodiversity loss is higher among aquatic insects, and caddisflies comprise one of the most vulnerable orders. In this way, research focusing on describing new caddisfly species and understanding their distribution ranges will increase knowledge of caddisfly biodiversity. In the past 14 years, the team from the Laboratório de Entomologia Aquática “Prof. Dr. Claudio Gilberto Froehlich” (LEAq, Universidade Federal da Bahia, Brazil) and collaborators have been addressing these issues, resulting in 55 described species of caddisflies. Taxa in other insect orders have also received attention and an additional 16 species have been described (eight mayflies, four stoneflies, four neuropteran spongillaflies, and antlions). Here, eight caddisfly species are described and illustrated (Atopsyche froehlichi sp. nov., Austrotinodes zeferina sp. nov., Cernotina kariri sp. nov., Neoathripsodes froehlichi sp. nov., Notalina claudiofroehlichi sp. nov., Oecetis marcus sp. nov., Phylloicus froehlichi sp. nov., and Polycentropus claudioi sp. nov.), five of them in honor of LEAq’s patron. In addition, new distributional data are presented for 10 known species. A checklist of the caddisfly fauna of Bahia state is also presented, with 138 species, around 75% and 30% of them recorded and described by the LEAq team, respectively. Currently, for caddisflies, Bahia is the fifth most species-rich state in Brazil, and the first in the Brazilian Northeast region.

ABSTRACT Plant-growth-promoting bacteria of the genus Azospirillum and Herbaspirillum can improve crop yields of maize in tropical environments. The main mechanism proposed for its promotion is related to the auxins produced by these species. The aim of this study was to compare the maize growth response of inoculation using two species, Azospirillum baldaniorum Sp245 (Ab-Sp245) and Herbaspirillum seropedicae ZAE94 (Hs-ZAE94) during the initial growth under controlled conditions. A pot experiment was conducted with five harvests measuring bacterial counts, root morphology, biomass, and nitrogen content, and soluble metabolites for 38 days. Depending on the harvest period, a differential growth response between the two strains test was compared to the control. Plants use the bacterium applied to improve nitrogen acquisition and transform it into higher biomass and nitrogen accumulation, especially for Ab-Sp245. On the other side, Hs-ZAE94 altered the soluble metabolites resulting in higher NO3-, NH4+, and N-amino and lower soluble fractions compared to Ab-Sp245 and the control. The observed plant-bacteria interaction is closely fine-tuned and regulated by the strain used and differences in growth promotion effects cannot solely attributed to the root morphology.

ABSTRACT Objective: To evaluate the role of music as a distraction technique to minimize anxiety during dental care in children aged 7 to 9 years when submitted to local anesthetic procedure. Method: This study was carried out in the Dentistry Module of the State University of Southwestern Bahia, located in the city of Jequié, Bahia, Brazil. It was performed through the application of the Facial Image Scale, measurement of heart rate and blood pressure with the aid of the digital sphygmomanometer at two different times during pediatric dentistry care with and without the influence of classical music. Data were tabulated in the Statistical Package for Social Sciences software, version 21.0, expressed as medians and interquartile range. For inferential statistics, the Shapiro-Wilk normality test was used and the analysis of the effect of music was calculated using the paired T-tests and Wilcoxon, considering a significance level of 95%. Results: The sample consisted of 7 children, mostly male, with a median of 8 years of age. Regarding the psychophysiological variables, no significant variations were observed compared to before and after in the groups with music and without music. Conclusion: In this study, no differences were found regarding the reduction of anxiety in the group in which music was used. Additional studies with a representative sample are needed.

RESUMO Objetivo: avaliar o papel da música como técnica de distração para minimizar a ansiedade durante o atendimento odontológico em crianças de 7 a 9 anos de idade quando submetidas a procedimento anestésico local. Métodos: Este estudo foi realizado no Módulo de Odontologia da Universidade Estadual do Sudoeste da Bahia, situado na cidade de Jequié – BA. Foi realizado a partir da aplicação da Facial Image Scale, mensuração da frequência cardíaca e pressão arterial com auxílio do esfigmomanômetro digital em dois momentos distintos ao longo do atendimento em odontopediatria com e sem a influência da música clássica. Os dados foram tabulados no software Statistical Package for Social Sciences, versão 21.0, expressos como medianas e intervalo interquartílico. Para a estatística inferencial, utilizou-se o teste de normalidade Shapiro-Wilk e a análise do efeito da música foi calculada utilizando os testes T-pareado e o Wilcoxon, considerando nível de significância de 95%. Resultados: A amostra foi constituída por 7 crianças, pertencendo sua maioria ao sexo masculino, com uma mediana de 8 anos de idade. Em relação às variáveis psicofisiológicas, não foram observadas variações significativas em comparação ao antes e depois nos grupos com música e sem música. Conclusões: Neste estudo não foram encontradas diferenças no que diz respeito à redução da ansiedade no grupo em que a música foi utilizada, havendo necessidade de estudos adicionais, com amostra representativa.

Abstract Objective: Compare the occurrence of choking and gagging in infants subjected to three complementary feeding (CF) methods. Methods: Randomized clinical trial with mother-infant pairs, allocated according to the following methods of CF: a) Parent-Led Weaning (PLW) — group control, b) Baby-Led Introduction to SolidS (BLISS), and c) mixed (initially BLISS and if the infant presents a lack of interest or dissatisfaction, PLW), with the last two methods guided by the infant. Mothers received nutritional intervention on CF and prevention of choking and gagging according to the method at 5.5 months of age and remained in follow-up until 12 months. Frequencies of choking and gagging were collected by questionnaire at nine and 12 months. The comparison between groups was performed using the analysis of variance test (p < 0.05). Results: 130 infants were followed, and 34 (26.2%) children presented choking between six and 12 months of age, 13 (30.2%) in PLW, 10 (22.2%) BLISS, and 11 (26.2%) mixed method, no significative difference between methods (p > 0.05). The choking was caused mainly by the semi-solid/solid consistency. Moreover, 100 (80%) infants aged from six to 12 months presented gagging and their characteristics were not statistically different among groups (p > 0.05). Conclusion: Infants following a baby-led feeding method that includes advice on minimizing choking risk do not seem more likely to choke than infants following traditional feeding practice that includes advice on minimizing choking risk.

RESUMO Objetivo: Estimar a prevalência de aleitamento materno exclusivo (AME), introdução de água, chás ou outros leites, bem como identificar os fatores associados à interrupção do AME aos 30 dias de vida. Métodos: Estudo transversal que utilizou questionários estruturados e pré-testados, aplicados a 310 mães em dois momentos: presencialmente, na maternidade, e aos 30 dias de vida da criança, mediante ligação telefônica. Estatística descritiva e regressão multivariada de Poisson, seguindo modelo hierárquico multiníveis conforme a proximidade com o desfecho, estimaram a associação entre as variáveis dependentes e independentes. Resultados: A manutenção do AME aos 30 dias de idade da criança ocorreu em 85,2% da amostra, e 1,6 % receberam água, 11,5 % chás e 8,2% outro leite. Preditores para a interrupção do AME na análise univariada foram o retorno das mães ao trabalho ou estudo logo após o nascimento do bebê (razão de incidência — RI 2,88; intervalo de confiança — IC95% 1,14–7,25) e o uso de chupeta (RI 3,29; IC95% 1,52–6,22). A interrupção do AME foi menor no grupo de participantes que recebeu apoio da avó materna do lactente (RI 2,71; IC95% 1,11–6,56) e do companheiro (RI 4,78; IC95% 1,90–12,06). Após a análise multivariada, apenas o uso de chupeta (RI 5,47; IC95% 2,38–19,3) e o apoio do companheiro (RI 6,87; IC95% 2,04–23,1) mantiveram associação com o desfecho. Conclusões: A prevalência de AME encontrada neste estudo pode ser considerada boa, e futuras intervenções que visem ao aumento da duração do AME nessa população devem levar em consideração a participação do companheiro e o reforço para a não introdução da chupeta.

ABSTRACT Objective: To estimate the prevalence of exclusive breastfeeding (EBF), introduction of water, herbal teas, or other milks, as well as to identify the factors associated with the interruption of EBF at the 30th day after birth. Methods: This is a cross-sectional study using structured and pretested questionnaires applied to 310 mothers in two moments: in person, at the maternity ward; and at the time the infant was 30 days of age, by telephone call. Descriptive statistics and multivariate Poisson regression, following a multilevel hierarchical model according to the proximity to the outcome, were used to estimate the association between dependent and independent variables. Results: The maintenance of EBF at 30 days of age of the infant occurred in 85.2% of the sample, 1.6% receiving water, 11.5% herbal teas, and 8.2% other milk. Predictors for EBF interruption in the univariate analysis were the mothers’ return to work or study activities shortly after the baby's birth (IR 2.88; 95%CI 1.14–7.25) and the use of a pacifier (IR 3.29; 95%CI 1.52–6.22). The interruption of EBF was lower in the group of participants who received support from the infant's maternal grandmother (IR 2.71; 95%CI 1.11–6.56) and their partner (IR 4.78; 95%CI 1.90–12.06). After a multivariate analysis, only the use of a pacifier (IR 5.47; 95%CI 2.38–19.3) and the partner's support (IR 6.87; 95%CI 2.04–23.1) maintained the association with the outcome. Conclusions: The prevalence of EBF found in this study can be considered good, and future interventions aimed at increasing the duration of EBF in this population should take into account the participation of the partner and the reinforcement for not introducing the pacifier.

RESUMO: Os organismos da macrofauna edáfica são fundamentais para a manutenção da qualidade do solo. O objetivo deste trabalho foi caracterizar a macrofauna edáfica sob diferentes espécies de plantas de cobertura, incluindo monocultura ou cultivo consorciado associados a dois tipos de manejo do solo no sudoeste do Piauí. O estudo foi realizado em Latossolo Amarelo (Sistema Brasileiro de Classificação de Solos) no município de Bom Jesus, Piauí, distribuídos em parcelas de 30 m2. O experimento e avaliação da macrofauna edáfica foram conduzidos em um ensaio fatorial em faixas 9 × 2, com combinações entre culturas /consórcios de cobertura e manejo do solo (com ou sem preparo), com quatro repetições. Os monólitos de solo (0,25 × 0,25 m) foram retirados aleatoriamente de cada parcela, para contagem da macrofauna, nas camadas de 0‒0,1, 0,1‒0,2, e 0,2-0,3 m de profundidade, inclusive liteira de superfície. Após a identificação e contagem dos organismos, foi determinada a densidade relativa de cada táxon em cada profundidade. A abundância total da macrofauna edáfica quantificada no experimento foi de 2.408 ind.m-2, distribuídos em 6 classes, 16 ordens e 31 famílias. Os resultados da análise multivariada revelaram que espécies de gramíneas em sistemas de cultivo solteiro e plantio direto favoreceram maior densidade da macrofauna, em especial do grupo taxonômico Isoptera. A ausência de preparo também proporcionou maior riqueza de famílias, destacando-se o grupo taxonômico Coleoptera adulto em plantio direto e Hemiptera em plantio convencional.

ABSTRACT: The soil macrofauna is fundamental for the maintenance of soil quality. The aim of this study was to characterize the soil macrofauna under different species of cover crops, including monoculture or intercropping associated to two types of soil management in the southwest region of Piauí state. The study was carried out in an Oxisol (Latossolo Amarelo, according to Brazilian Soil Classification System) in the municipality of Bom Jesus, Piauí, distributed in 30 m2 plots. Testing and evaluation of the soil macrofauna were conducted in a 9 × 2 strip factorial design, with combinations between cover crops/consortia and soil management (with or without tillage), with four replications. Soil monoliths (0.25 × 0.25 m) were randomly sampled in each plot for macrofauna at 0‒0.1, 0.1‒0.2, and 0.2‒0.3 m depth, including surface litter. After identification and counting of soil organims, the relative density of each taxon in each depth was determined. The total abundance of soil macrofauna quantified under cover crops in the conventional and no-tillage system was 2,408 ind. m-2, distributed in 6 classes, 16 orders, and 31 families. The results of multivariate analysis show that grass species in sole cropping systems and no-tillage presents higher macrofauna density, in particular the taxonomic group Isoptera. No-tillage also provided higher richness of families, where Coleoptera adult were the second more abundant group in no-tillage and Hemiptera in conventional tillage.

Resumo: O objetivo deste trabalho foi avaliar as características morfogenéticas, estruturais, fisiológicas e produtivas do amendoim-forrageiro submetido a diferentes níveis de sombreamento artificial no campo. O amendoim-forrageiro 'Amarillo MG-100' foi plantado em abril de 2015, e as avaliações foram realizadas de maio de 2017 a abril de 2018. Os tratamentos foram: 0, 30, 45 e 75% de sombreamento artificial. Não houve efeito significativo do sombreamento sobre as características morfogenéticas do amendoim-forrageiro. O sombreamento aumentou o comprimento final da folha e a altura do dossel e diminuiu o índice de área foliar e o número de estolões. A fotossíntese, a taxa de transpiração, a condutância estomática, a clorofila b e a temperatura das folhas aumentaram quadraticamente em função do sombreamento. Houve efeito linear positivo sobre o tamanho do ciclo de crescimento e efeito quadrático sobre a produção de massa de matéria seca, com o máximo a 30% de sombra. O amendoim-forrageiro colhido a 95% de interceptação luminosa (11 cm de altura) apresenta adaptação ao sombreamento de até 45%, com aumento do tamanho das folhas, da altura do dossel, da taxa fotossintética, da condutância estomática, da taxa de transpiração e da clorofila b. Além disso, as plantas sob sombreamento de 30% apresentam maior rendimento do que aquelas que crescem sob a luz do sol.

Abstract: The objective of this work was to evaluate the morphogenetic, structural, physiological, and productive traits of forage peanut (Arachis pintoi) subjected to different levels of artificial shading in the field. The 'Amarillo MG-100' forage peanut was planted in April 2015, and the evaluations were carried out from May 2017 to April 2018. The treatments were: 0, 30, 45, and 75% of artificial shading. There was no significant effect of shading on the morphogenetic traits of forage peanut. Shading increased final leaf length and canopy height and decreased the leaf area index and number of stolons. Photosynthesis, transpiration rate, stomatal conductance, chlorophyll b, and leaf temperature increased quadratically as a function of shading. There was a linear positive effect on the growing cycle length and a quadratic effect on the production of dry matter mass, with the maximum at 30% shade. Forage peanut harvested at 95% light interception (11-cm height) shows adaptation to shading up to 45%, with increased leaf size, canopy height, photosynthetic rate, stomatal conductance, transpiration rate, and chlorophyll b. In addition, plants at 30% shading show a higher yield than those growing under full sun.

The ToF-SIMS technique was applied to analyze thin ZrO2 coatings deposited on the surface of a Ni-Ti alloy. Due to its nanostructured nature, these films are difficult to characterize by conventional techniques. ZrO2 coatings were deposited on near equiatomic Ni-Ti wires by pulse electrodeposition. Part of the samples was electrolytically polished before the coating process. The coated samples were then analyzed by ToF-SIMS to evaluate the influence of the deposition time and previous surface electropolishing on the structure of the deposited coating. The results indicate that thicker coatings were produced on the electropolished samples, in comparison with the as-received ones. The best uniformity in thickness was achieved when Ni-Ti samples were previously electropolished followed by ZrO2 electrodeposition for 1200 seconds. Moreover, it was possible to observe by this technique that the inclusions in the Ni-Ti matrix were not entirely covered by the coating.

ABSTRACT The use of dark septate fungi (DSE) to promote plant growth can be beneficial to agriculture, and these organisms are important allies in the search for sustainable agriculture practices. This study investigates the contribution of dark septate fungi to the absorption of nutrients by rice plants and their ensuing growth. Four dark septate fungi isolates that were identified by Internal transcribed spacer phylogeny were inoculated in rice seeds (Cv. Piauí). The resulting root colonization was estimated and the kinetic parameters Vmax and Km were calculated from the nitrate contents of the nutrient solution. The macronutrient levels in the shoots, and the NO3--N, NH4+-N, free amino-N and soluble sugars in the roots, sheathes and leaves were measured. The rice roots were significantly colonized by all of the fungi, but in particular, isolate A103 increased the fresh and dry biomass of the shoots and the number of tillers per plant, amino-N, and soluble sugars as well as the N, P, K, Mg and S contents in comparison with the control treatment. When inoculated with isolates A103 and A101, the plants presented lower Km values, indicating affinity increases for NO3--N absorption. Therefore, the A103 Pleosporales fungus presented the highest potential for the promotion of rice plant growth, increasing the tillering and nutrients uptake, especially N (due to an enhanced affinity for N uptake) and P.

Resumo Objetivo: Avaliar uma possível associação entre presença de cistos renais simples (CRS) e aneurisma aórtico abdominal (AAA). Método: Em um estudo de caso versus controle com sujeitos com idade > 50 anos, avaliamos a prevalência de CRS detectados por tomografia computadorizada (TC). Comparamos os achados de 91 pacientes consecutivos com AAA oriundos da Divisão de Cirurgia Vascular com 396 pacientes sem AAA, randomicamente selecionados e ajustados por idade e gênero dentre 3.186 pacientes consecutivos que se submeteram a TC abdominal. Cisto simples foi definido como lesão hipodensa oval ou arredondada com paredes finas, maiores do que 4 mm em TC sem realce contrastual ou septação. Pacientes foram considerados com AAA quando o diâmetro da aorta era maior que 3,0 cm. Resultados: Pacientes com AAA e sem AAA eram semelhantes quanto a idade (67,9±8,41 vs. 68,5±9,13 anos) (p=0,889) e gênero (71,4 vs. 71,2% dos indivíduos masculinos, respectivamente) (p=0,999). Não havia diferença de prevalência de CRS entre casos e controles. Dentre indivíduos com AAA, 38 (41,8%; [IC95% 32,5-52,6]) tinham cistos renais, comparados com 148 (37,4%; [IC95% 32,7-42,2]) no grupo controle (p=0,473), com uma razão de prevalência (RP) de 1,16 (IC95% 0,80-1,68). Conclusão: Não observamos diferenças significativas na prevalência de CRS entre pacientes com AAA e controles. Nossos resultados sugerem que presença de CRS não é fator de risco ou preditor para AAA.

Summary Objective: To investigate the positive association between the presence of simple renal cysts (SRCs) and abdominal aortic aneurysm (AAA). Method: In a retrospective case-control study including subjects aged > 50 years, we evaluated the incidence of SRCs on computed tomography (CT) scan. We compared 91 consecutive patients with AAA referred from the Division of Vascular Surgery and 396 patients without AAA, randomly selected after being matched by age and gender from 3,186 consecutive patients who underwent abdominal CT. SRC was defined as a round or oval low-attenuation lesion with a thin wall and size > 4 mm on CT without obvious evidence of radiographic enhancement or septations. Patients were considered as having AAA if the size of aorta was greater than 3.0 cm. Results: Patients with AAA and without AAA were similar in terms of age (67.9± 8.41 vs. 68.5±9.13 years) (p=0.889) and gender (71.4 vs. 71.2% of male subjects, respectively) (p=0.999). There was no difference in the prevalence of SRC between case and controls. Among individuals with AAA, 38 (41.8%; [95CI 32.5-52.6]) had renal cysts compared to 148 (37.4%; [95CI 32.7-42.2]) in the control group (p=0.473), with a prevalence ratio (PR) of 1.16 (95CI 0.80-1.68). Conclusion: We found no significant differences in the prevalence of SRCs among patients with AAA and controls. Our findings suggest that the presence of SRCs is not a risk factor or a marker for AAA.

RESUMO A realização e difusão dos trabalhos referentes ao desempenho de plantas de cobertura em sistemas produtivos de culturas anuais no Piauí são importantes na busca por aumento de produtividade, maior eficiência no uso de fertilizantes e mitigação dos impactos ambientais. O objetivo deste trabalho foi avaliar o desempenho de plantas de cobertura na produção de fitomassa, acúmulo e liberação de nutrientes, durante o período de safra (novembro à abril), em Latossolo Vermelho distrófico no Cerrado piauiense em sistemas de produção com soja, milho e arroz de sequeiro. O experimento foi instalado nas safras agrícola de 2010/11 e 2011/2012 no município de Bom Jesus, PI. O delineamento utilizado foi o de blocos casualizados, em parcelas subdivididas. Nas parcelas estavam os sistemas de manejo do solo (preparo convencional e plantio direto) e nas subparcelas, os sistemas de produção com uso de plantas de cobertura na entressafra intercaladas com culturas anuais. Os sistemas com uso de Urochloa ruziziensis e Pennisetum glaucum semeadas em sobressemeadura na soja e U. ruziziensis consorciado de forma simultânea com milho apresentam-se como melhores opções na produção de fitomassa, acúmulo e liberação de nutrientes. As culturas do arroz e milho apresentam redução significativa na produtividade em áreas sob plantio direto recém-implantadas.

ABSTRACT Research on the performance of cover crops in crop systems of annual crops in the Brazilian state of Piauí contributes to increases in yield, greater efficiency of fertilizers and mitigation of environmental impacts. The aim of this study was to evaluate the performance of cover crops in terms of biomass production and the accumulation and release of nutrients during the crop season (November to April) in an oxisol in the Brazilian Cerrado in the state of Piauí that was submitted to different crop production systems including soybeans, maize and upland rice. The experiment was established during the 2010/11 and 2011/12 crop years in the rural area near the municipality of Bom Jesus, Piauí. The experimental design was a randomized block in a split-plot array. Different soil management systems (conventional and no-till) were evaluated in the main plots. Different crop production systems consisting of cover crops sown in the off-season (April to November), and annual crops sown during the crop season (November to April) were implemented in the subplots. The crop production systems that included Urochloa ruziziensis and Pennisetum glaucum overseeded on soybeans and Urochloa ruziziensis simultaneously intercropped with maize stood out in terms of biomass production and the accumulation and release of nutrients. Yields of maize and upland rice declined when sown under newly implemented no-till soil management.

For the biomedical application of NiTi alloys, an excellent surface finishing process is required to guarantee high corrosion resistance and biocompatibility, eliminating the allergenic and toxic effects associated with the release of nickel ions in the body. Electropolishing is a process that can reduce surface imperfections and form a thin protective layer of TiO2, even in complex-shaped devices. The main objective of our study was to find and report suitable parameters for electrolytic polishing of NiTi wires, in both the superelastic and shape memory states. The results indicate that electropolishing in a 3.5 mol•L-1 methanolic H2SO4 electrolyte at 20ºC can effectively reduce surface roughness, remove superficial nickel-rich layers and improve corrosion resistance for austenitic and martensitic NiTi alloys.

Resumo Atualmente, no cenário mundial e no Brasil, registra-se um aumento do número de diagnósticos de HIV/aids em idosos. Este artigo apresenta resultados de uma pesquisa qualitativa que buscou analisar a atuação de profissionais de saúde em idosos com diagnóstico de HIV/aids em um serviço público de saúde. Foram entrevistados os nove profissionais que compõem um serviço público de assistência especializada em HIV/aids de um município de porte médio de Minas Gerais. As falas foram submetidas à análise de conteúdo e a análise dos resultados permite afirmar que, na percepção dos profissionais de saúde, os principais impactos do diagnóstico de HIV/aids estão vinculados ao isolamento, solidão, preconceito, medo da revelação do diagnóstico e desaceleração ou interrupção das práticas sexuais. Os profissionais relatam sobrecarga de trabalho e sobrecarga psíquica, dificuldades em abordar aspectos da sexualidade e práticas sexuais com idosos e admitem compartilhar alguns estereótipos e preconceitos vinculados ao HIV/aids e à sexualidade da pessoa idosa. Pela análise dos resultados, pode-se concluir que os estigmas e preconceitos vinculados ao HIV e à sexualidade da pessoa idosa estão intimamente presentes no processo de trabalho dos profissionais entrevistados, impactam o tratamento e interferem nos processos de saúde e adoecimento. A discussão sobre esses aspectos deve compor as ações de formação em saúde.

Abstract The number of HIV/AIDs diagnoses among the elderly is currently increasing both in Brazil and on a global level. The present article describes the results of a qualitative study which aimed to assess the role of health professionals on elderly patients diagnosed with HIV/AIDS receiving treatment via the public health service. Nine professionals who made up a specialized HIV/AIDS service in a medium-sized city in the state of Minas Gerais were interviewed. Their statements were subjected to content analysis, and the results suggested that according to the perceptions of the health professionals, the main impacts of the diagnosis of HIV/AIDS are linked to isolation, loneliness, prejudice, fear of revealing the diagnosis and the decrease or interruption of sexual practices. The professionals reported being overburdened both psychologically and in terms of workload, having difficulty addressing aspects of sexuality and sexual practices with the elderly, and admitted to possessing certain stereotypes and prejudices related to HIV/AIDS and the sexuality of the elderly. Through results analysis, it was concluded that the stigma and prejudice related to HIV and the sexuality of the elderly are intimately present in the work processes of the professionals interviewed, impacting on the treatment of such individuals and interfering with their health and illness processes. The discussion of these aspects should be included in health training strategies.

Resumo Este estudo investigou a ocorrência de anticorpos anti-Neospora caninum e anti-Toxoplasma gondii por ensaio imunoenzimático indireto (ELISA) e reação de imunofluorescência indireta (RIFI), assim como os fatores de risco associados à toxoplasmose e à neosporose em 204 cães provenientes de áreas urbana e rural do município de Araguaína, Estado de Tocantins, Brasil. Cento e trinta amostras (63,7%) foram positivas para T. gondii, destas 57,1% e 70,7% oriundas de áreas urbanas e rurais, respectivamente. Considerando-se o teste RIFI, a frequência de soropositividade para T. gondii foi de 57,4% com distribuição de 62,6% e 52,4% entre áreas rurais e urbanas, respectivamente. Fatores associados à toxoplasmose canina foram raça e idade, com soropositividade maior para animais mais velhos (p<0,05). Em relação à N. caninum, 88 (43,1%) amostras foram positivas, segundo ELISA, sendo distribuídas em 42,9% para área urbana e 43,3% para área rural. Por meio da RIFI, anticorpos anti-N. caninum foram detectados em 62 (30,4%) cães, sendo distribuídos em 31,3% e 29,5% entre áreas rurais e urbanas, respectivamente. Os fatores associados à ocorrência de neosporose, pela RIFI, foram idade e raça (p<0,05). Este estudo representa a primeira detecção de anticorpos IgG para toxoplasmose e neosporose canina no Estado de Tocantins e evidencia a importância de cães na cadeia epidemiológica dessas doenças.

Abstract This study investigated occurrences of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies by means of the enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence assay (IFAT), along with risk factors associated with toxoplasmosis and neosporosis, in 204 dogs from urban and rural areas of the municipality of Araguaína, state of Tocantins, Brazil. One hundred and thirty samples (63.7%) were positive for T. gondii using ELISA: 57.1% and 70.7% in the urban and rural areas, respectively. The seropositivity frequency for T. gondii observed through IFAT was 57.4%, distributed between rural and urban areas as 62.6% and 52.4%, respectively. The factors associated with canine toxoplasmosis were age and breed (p<0.05). In relation to N. caninum, 88 samples (43.1%) were positive, according to ELISA, distributed as 42.9% in urban areas and 43.3% in rural areas. Anti - N. caninum antibodies were detected through IFAT in 62 dogs (30.4%), distributed as 31.3% and 29.5% between rural and urban areas, respectively. Age and breed were associated with neosporosis occurrence (p<0.05) by IFAT. This study provides the first detection of IgG antibodies for canine toxoplasmosis and neosporosis in the state of Tocantins and highlights the importance of dogs in the epidemiological chain of these diseases.

O atual avanço do número de pessoas idosas infectadas pelo vírus da imunodeficiência humana (HIV) representa um desafio aos profissionais de saúde, às políticas públicas e à população em geral no que concerne à necessidade de discutir sexualidade, práticas sexuais no processo de envelhecimento e o impacto dessas questões na promoção de saúde da pessoa idosa. Esta pesquisa teve como objetivo identificar os impactos psicossociais do diagnóstico de HIV/aids em pessoas idosas em atendimento em um serviço público de saúde, a partir da percepção dos sujeitos entrevistados. O estudo caracterizou-se como uma pesquisa qualitativa de caráter exploratório. Foram realizadas entrevistas individuais semiestruturadas com pacientes idosos soropositivos para HIV, residentes na macrorregião Centro-Oeste de Minas Gerais. A análise dos resultados permite afirmar que, de acordo com os 14 pacientes entrevistados, o diagnóstico de HIV/aids implica uma reorganização na maneira como se relacionam com pessoas; no autocuidado; nas práticas sexuais; na realização de atividades cotidianas e nas possibilidades de manterem sua participação em grupos sociais. Relatam constrangimento, medo da rejeição e discriminação, experiências de isolamento e preconceitos enfrentados. Com base na análise dos resultados, pode-se concluir que a maioria dos problemas enfrentados pelos idosos está vinculada aos estigmas e estereótipos socialmente construídos em relação à doença e aos esforços empreendidos pelos mesmos para manter o diagnóstico em segredo.

The current increase in the number of elderly people infected with the human immunodeficiency virus (HIV) represents a major challenge to healthcare professionals, public policies and the general population, in terms of the need to discuss the role of sexuality and sexual practices in the aging process and the impact of these issues on health promotion for the elderly. The aim of the present study was to identify the psychosocial impact of HIV/Aids diagnosis on elderly persons receiving care in a public healthcare service, based on the perception of the individuals themselves. A qualitative research study of an exploratory nature was performed, based on individual semi-structured interviews with HIV-positive elderly patients, living in the mid-west macro-region of Minas Gerais. Analysis of the results suggested that, according to the 14 patients interviewed, HIV diagnosis involves changes in the way they relate to people; in self-care; in sexual practices; in the performance of daily activities and in the possibility of continuing to participate in social groups. The patients reported embarrassment, fear of rejection and discrimination, and experiences of isolation and facing prejudice. Based on the results, it can be concluded that most of the problems faced by older people are linked to socially constructed stigmas and stereotypes associated with the disease and the efforts made by such individuals to keep the diagnosis a secret.