Pesquisa | SciELO

ABSTRACT Purpouse: One of the many artificial intelligence based tools that has gained popularity is the Chat-Generative Pre-Trained Transformer (ChatGPT). Due to its popularity, incorrect information provided by ChatGPT will have an impact on patient misinformation. Furthermore, it may cause misconduct as ChatGPT can mislead physicians on the decision-making pathway. Therefore, the aim of this study is to evaluate the accuracy and reproducibility of ChatGPT answers regarding urological diagnoses. Materials and Methods: ChatGPT 3.5 version was used. The questions asked for the program involved Primary Megaureter (pMU), Enuresis and Vesicoureteral Reflux (VUR). There were three queries for each topic. The queries were inserted twice, and both responses were recorded to examine the reproducibility of ChatGPT's answers. Afterwards, both answers were combined. Finally, those rwere evaluated qualitatively by a board of three specialists. A descriptive analysis was performed. Results and Conclusion: ChatGPT simulated general knowledge on the researched topics. Regarding Enuresis, the provided definition was partially correct, as the generic response allowed for misinterpretation. For VUR, the response was considered appropriate. For pMU it was partially correct, lacking essential aspects of its definition such as the diameter of the dilatation of the ureter. Unnecessary exams were suggested, for Enuresis and pMU. Regarding the treatment of the conditions mentioned, it specified treatments for Enuresis that are ineffective, such as bladder training. Therefore, ChatGPT responses present a combination of accurate information, but also incomplete, ambiguous and, occasionally, misleading details. Purpouse ChatGenerative Chat Generative PreTrained Pre Trained ChatGPT. . (ChatGPT) misinformation Furthermore decisionmaking decision making pathway Therefore diagnoses Methods 35 3 5 3. used pMU, , (pMU) VUR. VUR (VUR) topic twice ChatGPTs s Afterwards combined Finally specialists performed Conclusion topics correct misinterpretation appropriate ureter suggested mentioned ineffective training incomplete occasionally details (ChatGPT (pMU (VUR

https://doi.org/10.1590/s1677-5538.ibju.2023.0570

Evaluation of Intestinal Permeability of the Antifungal Compound PD76: Comparison of in silico Platforms and in vitro Assay in Caco-2 Cell Model PD76 PD Caco2 Caco 2 Caco- PD7
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Pierotte, Isabella C.

; Silva, Iara R.

; Santos Jr., Valtair S. dos

; Almeida, Gabriel P.

; Santos, Pedro Henrique G. dos

; Maltarollo, Vinícius G.

; Oliveira, Renata B. de

; Gonçalves, José Eduardo

; César, Isabela Costa

Journal of the Brazilian Chemical Society

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2024, Volume 35 Nº 7 elocation e-20240011

Resumo: En | Texto: En | PDF: En

The compound 2-hydrazinyl-4-(4-methoxyphenyl)thiazole (PD76) is a novel thiazolyl hydrazine derivative with proven antifungal activity against different fungal species, mainly Candida and Cryptococcus. Considering the advantages of oral route for clinical therapy, the aim of this work was to evaluate the potential intestinal permeability of this new antifungal drug. For the quantitation of PD76, a high-performance liquid chromatography method was developed and fully validated. The cytotoxicity of the compound in Caco-2 cells was analyzed and intestinal permeability of PD76 was assessed by means of the comparison of in vitro assay in Caco-2 cells and in silico platforms ADMETlab and admetSAR. Cell viability above 70% was obtained at all PD76 studied concentrations. Using Caco-2 cell model, the compound showed apparent permeability coefficients (Papp) of 5.25 × 10-6 and 23.28 × 10-6 cm s-1 in apical-basolateral and basolateral-apical directions, respectively. Experiments performed using verapamil as P-gp inhibitor demonstrated that PD76 is slightly susceptible to active efflux. Both in silico platforms inferred that PD76 presents permeability in Caco-2 cells, with Log P values of 2.82 (ADMETlab) and 2.10 (admetSAR). The results obtained in permeability studies showed that PD76 presents moderate intestinal permeability and a promising profile for clinical application. 2hydrazinyl44methoxyphenylthiazole hydrazinylmethoxyphenylthiazole 2 hydrazinyl 4 methoxyphenyl thiazole PD (PD76 species Cryptococcus therapy drug highperformance high performance validated Caco2 Caco Caco- PD7 admetSAR 70 concentrations model Papp (Papp 525 5 25 5.2 106 10 6 10- 2328 23 28 23.2 s1 s 1 s- apicalbasolateral apical basolateral basolateralapical directions respectively Pgp gp efflux 282 82 2.8 (ADMETlab 210 2.1 . (admetSAR) application methoxyphenylthiazole (PD7 7 52 5. 232 23. 8 2. 21 (admetSAR (PD

https://doi.org/10.21577/0103-5053.20240011

Calcium anacardate as source of phenolic compounds in diet for New Zealand White female rabbits
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Oliveira, José Vladmir Araujo

; Watanabe, Pedro Henrique

; Vieira Neto, Maurício Francisco

; Nascimento, Germano Augusto Jerônimo do

; Nepomuceno, Rafael Carlos

; Pacheco, Paulo Natanael Lima

; Santos, Marcelo Emersom Costa

; Freitas, Ednardo Rodrigues

Pesquisa Agropecuária Brasileira

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2024, Volume 59 elocation e03552

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo O objetivo deste trabalho foi avaliar o efeito da adição dietética de anacardato de cálcio como fonte de ácido anacárdico sobre o desempenho reprodutivo e os parâmetros antioxidantes sanguíneos de coelhas Nova Zelândia Branco. Vinte e sete fêmeas da raça Nova Zelândia Branco foram distribuídas em delineamento em blocos ao acaso, com três tratamentos e nove repetições, tendo-se considerado um animal como unidade experimental. Os tratamentos consistiram em rações peletizadas, com diferentes níveis de inclusão de anacardato de cálcio (0, 0,5 e 1,0%). A inclusão de anacardato de cálcio nas dietas aumentou a concentração de compostos fenólicos e a capacidade antioxidante no sangue das coelhas fêmeas lactantes. O anacardato de cálcio ao nível de 1,0% melhora a conversão alimentar durante a gestação e aumenta os compostos fenólicos e a capacidade antioxidante no soro sanguíneo das coelhas aos 21 dias de lactação. acaso repetições tendose tendo se experimental peletizadas 0, 0 (0 05 5 1,0%. 10 . 1 1,0%) lactantes 1,0 2 lactação ( 1,

Abstract The objective of this work was to evaluate the effect of the dietary addition of calcium anacardate as a source of phenolic compounds on the reproductive performance and blood antioxidant parameters of New Zealand White female rabbits. Twenty-seven New Zealand White female rabbits were distributed in a randomized complete block design with three treatments and nine replicates, considering one animal as the experimental unit. The treatments consisted of pelleted diets with different inclusion levels of calcium anacardate (0, 0.5, and 1.0%). The inclusion of calcium anacardate in the diets increased the concentration of phenolic compounds and the antioxidant capacity in the blood of lactating female rabbits. Calcium anacardate at the level of 1.0% improves the feed conversion ratio during gestation and increases the phenolic compounds and antioxidant capacity in the blood serum of female rabbits at 21 days of lactation. Twentyseven Twenty seven replicates unit 0, 0 (0 05 5 0.5 1.0%. 10 . 1 1.0%) 1.0 2 lactation ( 0. 1.

https://doi.org/10.1590/s1678-3921.pab2024.v59.03552

Nutrients intake, milk production, and production costs of dairy goats fed with high proportions of cactus cladodes genotypes Opuntia and Nopalea in association with different forage sources intake
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Silva, José Geraldo Medeiros da

; Santos, Lázaro Henrique de Medeiros

; Silva, Hildelblano Pereira da

; Silva, Gustavo José Azevedo Medeiros da

; Cardoso, Daniel Barros

; Lima, Guilherme Ferreira da Costa

; Melo, Airon Aparecido Silva de

Acta Scientiarum. Animal Sciences

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2024, Volume 46 elocation e62223

Resumo: En | Texto: En | PDF: En

ABSTRACT. This study aimed to evaluate the nutrient intake, milk production, and production costs of dairy goats fed diets containing the rations with cactus cladodes (Opuntia stricta or Nopalea cochenillifera) associated with silage (Sorghum bicolor) or Tifton hay (Cynodon spp). Twelve pluriparous Saanen and Anglo Nubiana goats (42.25 ± 5.48 kg) at eight weeks of lactation were used. Treatments lasted 60 days, consisting of four periods of 15 days, distributed in three Latin square (4×4) with four diets. The treatments represented by diets with different associated feed: SSOP (Sorghum Silage + Opuntia); THOP (Tifton hay + Opuntia); SSNO (Sorghum Silage + Nopalea); and THNO (Tifton hay + Nopalea), in addition to concentrate in all diets. There were differences (p < 0.05) for the intakes of DM, OM, with higher values observed for goats fed the diets THNO, THOP, and SSNO, and for CP, NDF and ADF were higher for THNO and SSNO diets. Similar behavior occurred for voluntary and total water intake. The milk production and feed efficiency did not differ (p > 0.05). Costs with food, milk revenue, and gross revenue were not influenced by diets (p > 0.05). The Opuntia or Nopalea cactus cladodes genotypes associated with sorghum silage or tifton hay can be used to feed dairy goats in the semiarid region. ABSTRACT intake cochenillifera Sorghum bicolor Cynodon spp. spp . spp) 42.25 4225 42 25 (42.2 548 5 48 5.4 kg 6 days 1 4×4 44 4 (4×4 Opuntia) Nopalea) Nopalea, , p 0.05 005 0 05 DM OM CP 0.05. food region 42.2 422 2 (42. 54 5. 4× (4× 0.0 00 42. (42 (4 0. (

https://doi.org/10.4025/actascianimsci.v46i1.62223

Effects of encapsulated butyric acid on general performance, intestinal health, and colonization in organs of poultry infected with Salmonella Enteritidis performance health
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Leonídio, Angélica Ribeiro Araújo

; Minafra, Cíntia

; Andrade, Maria Auxiliadora

; Nascente, Eduardo de Paula

; Nascimento, Gisele Mendanha

; Santos, Jardel Barbosa dos

; Stringhini, José Henrique

; Almeida, Ana Maria de Souza

Revista Brasileira de Zootecnia

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2024, Volume 53 elocation e20220150

Resumo: En | Texto: En | PDF: En

ABSTRACT This experiment was carried out to evaluate the effects of organic acids on the intestinal integrity and productive performance of broiler chickens experimentally inoculated with Salmonella Enteritidis. Additionally, Salmonella colonization levels in internal organs were evaluated. The study included 576 one-day-old CobbTM male broilers, distributed into eight treatment groups and six replicates. A completely randomized experimental design (encapsulated butyric acid × inoculation by Salmonella Enteritidis) was used. Poultry was orally inoculated with Salmonella Enteritidis in the first day (0 or 2.0 × 106 CFU/mL) and after 22 days of age (0 or 1.0 × 109 CFU/ mL). The butyric acid was added to the feed in different concentration (treatments 0.03, 0.075, and 0.15%), and the chickens were raised up to 42 days old. Encapsulated butyric acid at 0.03% increased the body weight gain and the average weight of the chickens up to 21 days old. After 14 days old, the butyric acid had a positive effect on jejunum villus. Encapsulated butyric acid at 0.03% in feed improves the performance and intestinal integrity of chickens. Additionally evaluated 57 onedayold one old broilers replicates encapsulated used 0 ( 20 2 2. 10 CFU/mL CFUmL CFU mL 1 1. mL. . mL) treatments 003 03 0.03 0075 075 0.075 0.15%, 015 0.15% , 15 0.15%) 4 villus 5 00 0.0 007 07 0.07 01 0.15 0. 0.1

https://doi.org/10.37496/rbz5320220150

Organofunctionalized Nb2O5 Nanoparticles for Photodynamic Therapy against A549 Cancer Cells NbO Nb O Nb2O A A54 A5
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Oliveira, Ronaldo C. S.

; Santos, Luiz Henrique C. dos

; Ghasemishahrestani, Zeinab

; Pereira, Marcos D.

; Forero, Josué Sebastián B.

; Simões, Grazieli

; Ribeiro, Emerson S.

; Corrêa, Rodrigo José

Journal of the Brazilian Chemical Society

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2024, Volume 35 Nº 11 elocation e-20240036

Resumo: En | Texto: En | PDF: En

Niobium pentoxide shows an interesting reactivity that allows the control of different aspects of its morphology and chemistry. In this study, Nb2O5 nanoparticles were modified with protoporphyrin IX (PPIX) and tris(ethynylphenyl) pyrene derivative (PyPh3) by using 3-aminopropyltriethoxysilane as linkage group and used as photosensitizers against lung cancer. The antitumor photoactivity against the A549 tumor cell line as a model of in vitro study showed half maximal inhibitory concentration (IC50) ca. 15 μmol L-1 for both materials and the absence of dark activity, indicating the viability of dye-modified Nb2O5 as a photodynamic therapy (PDT) agent. chemistry NbO Nb O Nb2O PPIX (PPIX trisethynylphenyl tris ethynylphenyl tris(ethynylphenyl PyPh3 PyPh (PyPh3 3aminopropyltriethoxysilane aminopropyltriethoxysilane 3 cancer A A54 IC50 IC (IC50 ca 1 L1 L L- activity dyemodified dye PDT (PDT agent (PyPh A5 IC5 (IC5 (IC

https://doi.org/10.21577/0103-5053.20240036

O TRABALHO ESCRAVO ENTRE OLHARES: UM DEBATE AMPLIADO SOBRE UM CAMPO CONSOLIDADO OLHARES
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Santos, José Henrique Lima

Almanack

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2024, Nº 36 elocation er00423

Resumo: Pt | Texto: Pt | PDF: Pt

https://doi.org/10.1590/2236-463336er00423

Diretriz de Avaliação Cardiovascular Perioperatória da Sociedade Brasileira de Cardiologia – 2024 202 20 2
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Gualandro, Danielle Menosi

; Fornari, Luciana Savoy

; Caramelli, Bruno

; Abizaid, Alexandre Antonio Cunha

; Gomes, Brenno Rizerio

; Tavares, Caio de Assis Moura

; Fernandes, Caio Julio Cesar dos Santos

; Polanczyk, Carisi Anne

; Jardim, Carlos

; Vieira, Carolina Leticia Zilli

; Pinho, Claudio

; Calderaro, Daniela

; Schreen, Dirk

; Marcondes-Braga, Fabiana Goulart

; Souza, Fábio de

; Cardozo, Francisco Akira Malta

; Tarasoutchi, Flavio

; Carmo, Gabriel Assis Lopes

; Kanhouche, Gabriel

; Lima, José Jayme Galvão de

; Bichuette, Luciana Dornfeld

; Sacilotto, Luciana

; Drager, Luciano Ferreira

; Vacanti, Luciano Janussi

; Gowdak, Luis Henrique Wolff

; Vieira, Marcelo Luiz Campos

; Martins, Marcelo Luiz Floriano Melo

; Lima, Márcio Silva Miguel

; Lottenberg, Marcos Pita

; Aliberti, Márlon Juliano Romero

; Marchi, Mauricio Felippi de Sá

; Paixão, Milena Ribeiro

; Oliveira Junior, Mucio Tavares de

; Yu, Pai Ching

; Cury, Patricia Ramos

; Farsky, Pedro Silvio

; Pessoa, Ranna Santos

; Siciliano, Rinaldo Focaccia

; Accorsi, Tarso Augusto Duenhas

; Correia, Vinícius Machado

; Mathias Junior, Wilson

Arquivos Brasileiros de Cardiologia

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2024, Volume 121 Nº 9 elocation e20240590

Resumo: Pt | Texto: Pt | PDF: Pt

https://doi.org/10.36660/abc.20240590

Importance of muscle strength to maintain mobility, but not to maintain postural balance in older women: Cross-sectional study mobility women Crosssectional Cross sectional
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Evangelista, Roberta Alexandra Gonçalves de Toledo

; Evangelista, Alexandre Lopes

; Ernandes, Rita de Cássia

; Brech, Guilherme Carlos

; Silva, Reinaldo Nonato da

; Lino, Matheus Henrique dos Santos

; Bocalini, Danilo Sales

; Graaf, Myriam de

; Mochizuki, Luis

; Soares-Junior, Jose Maria

; Baracat, Edmund Chada

; Greve, Júlia Maria D'Andréa

; Garcez-Leme, Luiz Eugênio

; Alonso, Angelica Castilho

Clinics

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2024, Volume 79 elocation 100504

Resumo: En | Texto: En | PDF: En

Abstract Background Acknowledging the relationship between postural control and muscle strength in lower limbs is important to find persons with high fall risk and to design fall prevention exercise programs. Objective To investigate the connection between knee muscle strength, semi-static postural balance, and functional mobility in irregularly active older women. Methods One hundred and ten older women aged 60 to 85 years underwent a semi-static postural balance test in a force and functional mobility measured by Timed Up & Go (TUG) with and without Cognitive Tasks (CT). Muscle strength of the knee was assessed by isokinetic dynamometry at 60°/s. Results A negative correlation between the functional mobility (TUG with and without CT) and the muscular strength of the knee was observed. Regarding semi-static balance, there was a low negative correlation with peak torque corrected for body weight of the extensors with the following variables with eyes open: Mean Velocity Dominant limb (D) (r = -0.19; p = 0.03); and anteroposterior range Non-Dominant limb (ND) (r = -0.19; p = 0.04); with eyes closed: mediolateral range ND limb (r = -0.21; p = 0.02) and Mean Velocity ND limb (r = -0.18; p = 0.05). Conclusions This study found that better functional mobility was associated with greater muscle strength in the knee in elderly women. There were weak negative correlations between knee extensor strength and some measures of semi-static postural balance, suggesting that different motor control actions are required to maintain semi-static balance and mobility. In the case of semi-static balance, a lower level of muscle response is required than in functional mobility. programs semistatic semi static 6 8 TUG CT. CT . (CT) 60s s 60°/s observed open D (D r 0.19 019 0 19 -0.19 0.03 003 03 0.03) NonDominant Non (ND 0.04 004 04 0.04) closed 0.21 021 21 -0.21 0.02 002 02 0.18 018 18 -0.18 0.05. 005 0.05 05 0.05) (CT 0.1 01 1 -0.1 0.0 00 0.2 2 -0.2 0. -0. -0 -

https://doi.org/10.1016/j.clinsp.2024.100504

10.

Serum progesterone profiles during the estrous cycle of dairy and beef cows determined using immunochemiluminescence (CLIA) and validated using enzyme-linked immunosorbent assay (ELISA) CLIA (CLIA enzymelinked enzyme linked ELISA (ELISA
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Padilha, Isabela da Silveira

; Kozicki, Luiz Ernandes

; Thomaz, Marina de Pauli

; Aita, Carlos Alberto Mayora

; Weber, Saulo Henrique

; Segui, Marcio Saporski

; Souza, Fernando Andrade

; Bergstein-Galan, Tacia Gomes

; Breda, José Carlos dos Santos

Ciência Animal Brasileira

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2024, Volume 25 elocation 76907E

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo O objetivo do estudo foi validar a metodologia da imunoquimioluminescência (CLIA) através da imunoabsorção enzimática (ELISA), determinando a concentração sérica de progesterona em vacas de leite e de corte, durante o ciclo estral. Foram empregadas 30 vacas multíparas não-prenhes (12 da raça Holandesa Preta e Branca e 18 Nelore). Vacas com corpo luteo foram escolhidas com vistas à sincronização do estro e da ovulação, mediante aplicação de 500 mcg de cloprostenol (im). Após a aplicação do luteolitico, os animais foram diariamente submetidos a exames de ultrasssonagrafia (US) ovariana para verificação da ovulação (=dia 01 do ciclo), bem como eram colhidas 02 amostras de sangue para a determinação da P4 pela CLIA e ELISA. As amostras eram centrifugadas para a obtenção do soro, e congeladas a -20 graus para posterior dosagem. Os valores de ELISA e CLIA foram comparados entre si mediante teste t pareado, regressão, ANOVA e coefficiente de determinação (R2), visando a verificação da sensibilidade e correspondência linear. A concentração de P4 originou perfis similares entre as duas metodologias; os perfis de P4 foram mais elevados em bovinos de corte, que nos de leite. A correspondência entre as metodologias resultou em elevado quociente de R2 nos perfis de P4. Concluiu-se que a CLIA pode ser empregada nas determinações hormonais da P4 serica bovina; a CLIA mostrou elevada correspondência linear com os valores da ELISA; a CLIA pode auxiliar as biotecnicas da reprodução nas determinações hormonais. (CLIA ELISA, , (ELISA) corte estral 3 nãoprenhes não prenhes 12 (1 1 Nelore. Nelore . Nelore) 50 im. im (im) luteolitico US (US =dia dia 0 ciclo, ciclo) P soro 20 -2 dosagem pareado regressão R2, R (R2) Concluiuse Concluiu se bovina (ELISA ( 5 (im 2 - (R2 (R

Abstract This study aimed to validate the methodology of immunochemiluminescence (CLIA) using an enzyme-linked immunosorbent assay (ELISA) to determine serum progesterone (P4) concentrations in non-pregnant multiparous dairy (12 Holstein cows) and beef (18 Nellore cows) cows during the estrous cycle. Cows with corpus luteum were chosen for estrus and ovulation synchronization by administering 500 mcg of cloprostenol. After luteolytic application, the animals were subjected to ovarian ultrasonography (US) daily to verify ovulation (day zero of the cycle) and two blood samples were collected for P4 determination using CLIA and ELISA. Samples were centrifuged to obtain serum and frozen at -20 °C for later measurement. ELISA and CLIA values were compared using the paired t-test, regression, analysis of variance, and coefficient of determination (R2) to verify sensitivity and linear correspondence. The P4 concentration determined by both methodologies showed a similar profile; the P4 profiles were higher in beef cattle than in dairy cattle. The correspondence between the methodologies resulted in a high quotient for R2 the P4 profiles. It was concluded that CLIA can be used for hormonal determinations of bovine serum P4. CLIA showed a high linear correspondence with ELISA values and can thus aid reproductive biotechnologies for hormone determination. (CLIA enzymelinked enzyme linked (ELISA P (P4 nonpregnant non pregnant 12 (1 18 cycle 50 cloprostenol application US (US day 20 -2 C measurement ttest, ttest t test, test t-test regression variance R (R2 profile (P 1 ( 5 2 - (R

https://doi.org/10.1590/1809-6891v25e-76907e

11.

Brazilian consensus recommendations on the diagnosis and treatment of autoimmune encephalitis in the adult and pediatric populations
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Dutra, Lívia Almeida

; Silva, Pedro Victor de Castro

; Ferreira, João Henrique Fregadolli

; Marques, Alexandre Coelho

; Toso, Fabio Fieni

; Vasconcelos, Claudia Cristina Ferreira

; Brum, Doralina Guimarães

; Pereira, Samira Luisa dos Apóstolos

; Adoni, Tarso

; Rocha, Leticia Januzi de Almeida

; Sampaio, Leticia Pereira de Brito

; Sousa, Nise Alessandra de Carvalho

; Paolilo, Renata Barbosa

; Pizzol, Angélica Dal

; Costa, Bruna Klein da

; Disserol, Caio César Diniz

; Pupe, Camila

; Valle, Daniel Almeida do

; Diniz, Denise Sisterolli

; Abrantes, Fabiano Ferreira de

; Schmidt, Felipe da Rocha

; Cendes, Fernando

; Oliveira, Francisco Tomaz Meneses de

; Martins, Gabriela Joca

; Silva, Guilherme Diogo

; Lin, Katia

; Pinto, Lécio Figueira

; Santos, Mara Lúcia Schimtz Ferreira

; Gonçalves, Marcus Vinícius Magno

; Krueger, Mariana Braatz

; Haziot, Michel Elyas Jung

; Barsottini, Orlando Graziani Povoas

; Nascimento, Osvaldo José Moreira do

; Nóbrega, Paulo Ribeiro

; Proveti, Priscilla Mara

; Castilhos, Raphael Machado do

; Daccach, Vanessa

; Glehn, Felipe von

Arquivos de Neuro-Psiquiatria

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2024, Volume 82 Nº 7 elocation s00441788586

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Antecedentes Encefalites autoimunes (EAIs) são um grupo de doenças inflamatórias caracterizadas pela presença de anticorpos contra antígenos neuronais e gliais, que ocasionam sintomas psiquiátricos subagudos, queixas de memória e distúrbios anormais do movimento. A maioria dos pacientes é jovem, e o atraso no tratamento está associado a pior prognóstico. Objetivo Com o apoio da Academia Brasileira de Neurologia (ABN) e da Sociedade Brasileira de Neurologia Infantil (SBNI), desenvolvemos um consenso sobre o diagnóstico e o tratamento da EAIs no Brasil utilizando a metodologia Delphi. Métodos Um total de 25 especialistas, incluindo neurologistas e neurologistas infantis, foram convidados a participar. Resultados Os especialistas concordaram que os pacientes com critérios de possíveis EAIs devem ser submetidos ao rastreio de anticorpos antineuronais no soro e no líquido cefalorraquidiano (LCR) por meio das técnicas de ensaio baseado em tecidos (tissue-based assay, TBA, em inglês) e ensaio baseado em células (cell-based assay, CBA, em inglês). As crianças também devem ser submetidas ao rastreio de de anticorpo contra a glicoproteína da mielina de oligodendrócitos (anti-myelin oligodendrocyte glycoprotein, anti-MOG, em inglês). O tratamento deve ser iniciado dentro das primeiras 4 semanas dos sintomas, sendo as opções de primeira linha metilprednisolona combinada com imunoglobulina intravenosa (IGIV) ou plasmaférese. O tratamento de segunda linha inclui rituximabe e ciclofosfamida. Bortezomib e tocilizumab são opções de tratamento de terceira linha. A maioria das crises epilépticas nas EAIs são sintomáticas, e os fármacos anticrise podem ser desmamadas após a fase aguda. Em relação à encefalite antirreceptor de N-metil-D-aspartato (anti-N-methyl-D-aspartate receptor, anti-NMDAR, em inglês), os especialistas concordaram que agentes imunossupressores orais não devem ser usados. Os pacientes devem ser avaliados na fase aguda e pós-aguda mediante escalas funcionais e cognitivas, como Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), Modified Rankin Scale (mRS), e Clinical Assessment Scale in Autoimmune Encephalitis (CASE). Conclusão Esta pesquisa oferece evidências tangíveis do manejo efetivo de pacientes com EAIs no sistema de saúde Brasileiro. (EAIs gliais subagudos movimento jovem prognóstico ABN (ABN SBNI, SBNI , (SBNI) Delphi 2 infantis participar LCR (LCR tissuebased tissue based assay TBA inglês cellbased cell CBA inglês. . antimyelin anti myelin glycoprotein antiMOG, antiMOG MOG, MOG anti-MOG IGIV (IGIV plasmaférese ciclofosfamida sintomáticas NmetilDaspartato N metil D aspartato antiNmethylDaspartate methyl aspartate receptor antiNMDAR, antiNMDAR NMDAR, NMDAR anti-NMDAR inglês, usados pósaguda pós cognitivas MiniMental Mini Mental MMSE, MMSE (MMSE) MoCA, MoCA (MoCA) mRS, mRS (mRS) CASE. CASE (CASE) Brasileiro (SBNI (MMSE (MoCA (mRS (CASE

Abstract Background Autoimmune encephalitis (AIE) is a group of inflammatory diseases characterized by the presence of antibodies against neuronal and glial antigens, leading to subacute psychiatric symptoms, memory complaints, and movement disorders. The patients are predominantly young, and delays in treatment are associated with worse prognosis. Objective With the support of the Brazilian Academy of Neurology (Academia Brasileira de Neurologia, ABN) and the Brazilian Society of Child Neurology (Sociedade Brasileira de Neurologia Infantil, SBNI), a consensus on the diagnosis and treatment of AIE in Brazil was developed using the Delphi method. Methods A total of 25 panelists, including adult and child neurologists, participated in the study. Results The panelists agreed that patients fulfilling criteria for possible AIE should be screened for antineuronal antibodies in the serum and cerebrospinal fluid (CSF) using the tissue-based assay (TBA) and cell-based assay (CBA) techniques. Children should also be screened for anti-myelin oligodendrocyte glucoprotein antibodies (anti-MOG). Treatment should be started within the first 4 weeks of symptoms. The first-line option is methylprednisolone plus intravenous immunoglobulin (IVIG) or plasmapheresis, the second-line includes rituximab and/or cyclophosphamide, while third-line treatment options are bortezomib and tocilizumab. Most seizures in AIE are symptomatic, and antiseizure medications may be weaned after the acute stage. In anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis, the panelists have agreed that oral immunosuppressant agents should not be used. Patients should be evaluated at the acute and postacute stages using functional and cognitive scales, such as the Mini-Mental State Examination (MMSE), the Montreal Cognitive Assessment (MoCA), the Modified Rankin Scale (mRS), and the Clinical Assessment Scale in Autoimmune Encephalitis (CASE). Conclusion The present study provides tangible evidence for the effective management of AIE patients within the Brazilian healthcare system. (AIE antigens symptoms complaints disorders young prognosis Academia ABN Sociedade Infantil SBNI, SBNI , SBNI) method 2 neurologists CSF (CSF tissuebased tissue based TBA (TBA cellbased cell CBA (CBA techniques antimyelin anti myelin antiMOG. antiMOG MOG . (anti-MOG) firstline line IVIG (IVIG plasmapheresis secondline second andor cyclophosphamide thirdline third tocilizumab symptomatic stage antiNmethylDaspartate N methyl D aspartate antiNMDAR NMDAR (anti-NMDAR used scales MiniMental Mini Mental MMSE, MMSE (MMSE) MoCA, MoCA (MoCA) mRS, mRS (mRS) CASE. CASE (CASE) system (anti-MOG (MMSE (MoCA (mRS (CASE

https://doi.org/10.1055/s-0044-1788586

12.

Allozyme and cytogenetic analysis in two species of Hypostomus (Siluriformes: Loricariidae) from the Paraguai River basin, Brazil: occurrence of B microchromosome and intrapopulation heterochromatic polymorphism in H. boulengeri Siluriformes (Siluriformes Loricariidae basin Brazil H
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Paiva, Suzana de

; Porto, Fernanda Errero

; Codognotto, Flávio José

; Fernandes, Carlos Alexandre

; Rossi, Margarida Maria Vieira

; Borin-Carvalho, Luciana Andreia

; Portela-Castro, Ana Luiza de Brito

; Zawadzki, Claudio Henrique

; Renesto, Erasmo

; Martins-Santos, Isabel Cristina

Neotropical Ichthyology

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2024, Volume 22 Nº 2 elocation e230117

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Hypostomus está distribuído por bacias da América Central e do Sul, com grande diversidade de espécies com conflitos taxonômicos. Desta forma, a integração de técnicas auxiliares contribui para a compreensão da sistemática e filogenia do grupo. Assim, este estudo teve como objetivo investigar Hypostomus cochliodon e H. boulengeri do riacho Onça (bacia do rio Paraguai) por meio de técnicas aloenzimáticas e citogenéticas. As análises citogenéticas em H. boulengeri mostraram número cromossômico igual a 2n = 68 (14m+14sm+18st+22a), sistema de NOR múltiplo revelado por Ag-NOR e 18S-FISH, um polimorfismo de heterocromatina em acrocêntricos e a presença de microcromossomos Bs. Hypostomus cochliodon mostrou um número diploide de 64 cromossomos (16m+26sm+14st+8a); apesar do sistema de NOR simples, alguns indivíduos apresentaram NOR em ambos os telômeros detectados por Ag-NOR e 18S-FISH. Uma isozima identificou dois loci diagnósticos (Idh-A and Gdh-A) entre as duas espécies e múltiplos loci com alelos únicos em H. boulengeri. A variabilidade genética indicada pela heterozigosidade média (He) foi de 0,2461 e 0,0309 em H. boulengeri e H. cochliodon, respectivamente. Assim, este estudo relata os primeiros dados citogenéticos para H. boulengeri e os primeiros dados isoenzimáticos para H. boulengeri e H. cochliodon. As duas espécies apresentaram evidentes diferenças citogenéticas e isoenzimáticas com a obtenção de marcadores genéticos exclusivos fornecendo suporte para futuros estudos evolutivos no grupo. Sul taxonômicos forma grupo Assim H bacia Paraguai n 6 14m+14sm+18st+22a, 14m14sm18st22a msmsta 14m+14sm+18st+22a , 14m 14sm 18st 22a m sm st (14m+14sm+18st+22a) AgNOR Ag 18SFISH, 18SFISH SFISH 18S FISH, FISH S 18S-FISH Bs 16m+26sm+14st+8a 16m26sm14st8a 16m 26sm 14st 8a (16m+26sm+14st+8a) simples 18SFISH. FISH. IdhA Idh GdhA Gdh Gdh-A He (He 02461 0 2461 0,246 00309 0309 0,030 respectivamente (14m+14sm+18st+22a (16m+26sm+14st+8a 0246 246 0,24 0030 030 0,03 024 24 0,2 003 03 0,0 02 2 0, 00

Abstract Hypostomus is distributed by Central and South America basins, with diverse species with taxonomic conflicts. This way, the integration of auxiliary techniques contributes to understanding the systematics and phylogeny of the group. Thus, this study aimed to investigate the Hypostomus cochliodon and H. boulengeri from the Onça stream (Paraguai River basin) by allozyme and cytogenetic techniques. Hypostomus boulengeri showed a diploid number of 68 chromosomes (14m+14sm+18st+22a), multiple NOR revealed by Ag-NOR and 18S rDNA FISH, a polymorphism of heterochromatin in acrocentrics and the presence of B microchromosome. Hypostomus cochliodon showed a diploid number of 64 chromosomes (16m+26sm+14st+8a); despite the single NOR, some individuals showed NOR in both telomeres detected by Ag-NOR and 18S rDNA FISH. Isozyme identified two diagnostic loci (Idh-A and Gdh-A) between the two species and multiple loci with unique alleles in H. boulengeri. The genetic variability indicated by the mean heterozygosity (He) was 0.2461 and 0.0309 in H. boulengeri and H. cochliodon,respectively.Thus, this study reports the first cytogenetic data for H. boulengeri and the first isozymatic data for H. boulengeri and H. cochliodon. The two species presented evident cytogenetic and isoenzymatic differences with the obtaining of exclusive genetic markers providing support for future evolutionary studies in the group. basins conflicts way group Thus H Paraguai basin 6 14m+14sm+18st+22a, 14m14sm18st22a msmsta 14m+14sm+18st+22a , 14m 14sm 18st 22a m sm st (14m+14sm+18st+22a) AgNOR Ag S FISH microchromosome 16m+26sm+14st+8a 16m26sm14st8a 16m 26sm 14st 8a (16m+26sm+14st+8a) IdhA Idh A GdhA Gdh Gdh-A He (He 02461 0 2461 0.246 00309 0309 0.030 cochliodonrespectivelyThus respectively cochliodon,respectively.Thus (14m+14sm+18st+22a (16m+26sm+14st+8a 0246 246 0.24 0030 030 0.03 024 24 0.2 003 03 0.0 02 2 0. 00

https://doi.org/10.1590/1982-0224-2023-0117

13.

HPV-positive women living in isolated areas in Amazonas, Brazil: Clinical–epidemiological profile and cytological findings HPVpositive HPV positive Amazonas Brazil Clinicalepidemiological Clinical epidemiological
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Mariño, Josiane Montanho

; Rocha, Danielle Albuquerque Pires

; Reis, Renato dos Santos

; Souza, Marly Gomes de

; Soares, Silvia Caroline Camargo

; Portugal, Jéssica Caroline Alves

; Reis, Marcelo Henrique dos

; Levi, José Eduardo

; Silva, Kátia Luz Torres

Cadernos Saúde Coletiva

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2024, Volume 32 Nº 1 elocation e32010365

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Introdução: O câncer cervical está associado com a infecção persistente pelo HPV (human papiloma virus) de alto risco e causa cerca de 265 mil mortes todos os anos no mundo, tornando-se um importante problema de saúde pública. Objetivo: Descrever o perfil epidemiológico, história clínica e achados citológicos de 55 mulheres infectadas com HPV vivendo em áreas remotas no Amazonas, Brasil. Método: Obtiveram-se as amostras por autocoleta, utilizando-se a escova Rovers® Evalyn® para detecção do HPV por meio da técnica de PCR (polymerase chain reaction). Realizou-se o exame citológico por citologia em meio líquido. Resultados: A média de idade das participantes foi de 35 anos de idade (DP=14). A maioria das mulheres apresentou baixa ou nenhuma escolaridade (52,7%), vivia em uma relação estável com um parceiro (74,5) e tinha de três a cinco filhos (32,5%). Observou-se o uso inconsistente de preservativo pela maioria das participantes (“nunca 34,5% e “às vezes” 41,8%). O exame citopatológico mostrou que 14 mulheres (25,4%) tinham alterações citológicas: 7 Ascus (50,0%), 5 L-SIL (35,7%) e 2 H-SIL (14,3%). Conclusões: Uma vez que as lesões cervicais são causadas pela infecção persistente pelo HPV, é importante que programas de prevenção do CC usem alternativas eficazes para acompanhar mulheres HPV positivas vivendo em áreas remotas. Introdução human virus 26 mundo tornandose tornando se pública Objetivo epidemiológico Amazonas Brasil Método Obtiveramse Obtiveram autocoleta utilizandose utilizando Rovers Evalyn polymerase reaction. reaction . reaction) Realizouse Realizou líquido Resultados 3 DP=14. DP14 DP DP=14 (DP=14) 52,7%, 527 52,7% , 52 (52,7%) 74,5 745 74 (74,5 32,5%. 325 32,5% 32 (32,5%) Observouse Observou nunca 345 34 34,5 às vezes 41,8%. 418 41,8% 41 8 41,8%) 1 25,4% 254 25 4 (25,4% citológicas 50,0%, 500 50,0% 50 0 (50,0%) LSIL L SIL 35,7% 357 (35,7% HSIL H 14,3%. 143 14,3% (14,3%) Conclusões DP1 DP=1 (DP=14 52,7 (52,7% 74, (74, 32,5 (32,5% 34, 41,8 25,4 (25,4 50,0 (50,0% 35,7 (35,7 14,3 (14,3% DP= (DP=1 52, (52,7 (74 32, (32,5 41, 25, (25, 50, (50,0 35, (35, 14, (14,3 (DP= (52, (7 (32, (25 (50, (35 (14, (DP (52 ( (32 (2 (50 (3 (14 (5 (1

Abstract Background: Cervical cancer (CC) is associated with persistent high-risk human papillomavirus (HPV) infection and it causes about 265,000 deaths per year worldwide, thus becoming an important public health problem. Objective: This study aimed to describe the epidemiological profile, clinical history, and cytological findings of 55 women infected with HPV living in remote areas in Amazonas, Brazil. Method: The samples were obtained by self-collection using the Rovers® Evalyn® Brush for HPV detection through PCR. The cytological examination was performed by liquid-based cytology. Results: The mean age of participants was 35 years (SD=14). Most of the women presented low or no schooling (52.7%), lived in stable relationship with a partner (74.5%), and had three to five children (32.7%). Inconsistent use of condoms by most of the participants (“never” — 34.5% and “sometimes” — 41.8%) was observed. Cytopathological examinations showed that 14 (25.4%) women had cytological alterations: 7 atypical squamous cells of undetermined significance (50.0%), 5 low-grade squamous intraepithelial lesions (35.7%), and 2 high-grade squamous intraepithelial lesions (14.3%). Conclusion: As cervical lesions have been caused by persistent HPV infection over the years, CC Prevention Programs use effective strategies to follow up on HPV-positive women living in remote areas. Background (CC highrisk high risk (HPV 265000 265 000 265,00 worldwide problem Objective profile history Amazonas Brazil Method selfcollection self collection Rovers Evalyn PCR liquidbased liquid based cytology Results 3 SD=14. SD14 SD SD=14 . (SD=14) 52.7%, 527 52.7% , 52 (52.7%) 74.5%, 745 74.5% 74 (74.5%) 32.7%. 327 32.7% 32 (32.7%) never (“never 345 34 34.5 sometimes “sometimes 41.8% 418 41 8 observed 1 25.4% 254 25 4 (25.4% alterations 50.0%, 500 50.0% 50 0 (50.0%) lowgrade grade 35.7%, 357 35.7% (35.7%) highgrade 14.3%. 143 14.3% (14.3%) Conclusion HPVpositive positive 26500 26 00 265,0 SD1 SD=1 (SD=14 52.7 (52.7% 74.5 (74.5% 32.7 (32.7% 34. 41.8 25.4 (25.4 50.0 (50.0% 35.7 (35.7% 14.3 (14.3% 2650 265, SD= (SD=1 52. (52.7 74. (74.5 32. (32.7 41. 25. (25. 50. (50.0 35. (35.7 14. (14.3 (SD= (52. (74. (32. (25 (50. (35. (14. (SD (52 (74 (32 (2 (50 (35 (14 (5 (7 (3 ( (1

https://doi.org/10.1590/1414-462x202432010365

14.

The decline in postural balance has a negative impact on the performance of functional tasks in individuals with Parkinson's Disease Parkinsons Parkinson s
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Luna, Natália Mariana Silva

; Bobbio, Tatiana Godoy

; Graaf, Myriam de

; Greve, Júlia Maria D'Andrea

; Ernandes, Rita de Cássia

; Dias, Aluane Silva

; Lino, Matheus Henrique dos Santos

; Soares-Junior, Jose Maria

; Baracat, Edmund Chada

; Mochizuki, Luis

; Brech, Guilherme Carlos

; Alonso, Angelica Castilho

Clinics

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2024, Volume 79 elocation 100382

Resumo: En | Texto: En | PDF: En

Abstract Introduction An accurate assessment of balance problems is critical for decreasing the risk of falling in patients with Parkinson's Disease (PD). Reliable diagnostic tools such as Computerized Dynamic Posturography (CDP) are not feasible for the clinical setting. Therefore, the present study's aim was to assess the correlation between the clinical Balance Evaluation Systems Test (BESTest) and CDP. Methods 20 male older adults with Parkinson's Disease (PD) were included in this study. Participants first executed the Sit-To-Stand (STS), Step/Quick turn (SQT), and Step Up and Over (SUO) tests on a Balance Master® force platform, followed by a clinical balance evaluation using the BESTest. Results Four outcomes of the CDP were negatively correlated with one or more BESTest domains or total BESTest score: STS sway velocity was negatively correlated with the anticipatory postural adjustment (p = 0.02) and sensory orientation (p = 0.01) domains. SQT turn time was negatively correlated with biomechanical restriction (for turns to the left, p = 0.01, and right, p = 0.03, respectively), postural response (p = 0.01, p = 0.01), dynamic balance during gait (p = 0.007, p = 0.001), and total score (p = 0.02, p = 0.01). Step over time to the right in SUP was negatively correlated with the limits of the stability domain (p = 0.002) and total BESTest score (p = 0.020). SUO impact index was negatively correlated with the anticipatory postural adjustment domain (p = 0.01). Conclusion This study shows that several BESTest domains are significantly correlated with CDP outcomes, demonstrating that the BESTest can be used as a more clinically feasible alternative for computerized posturography, without loss of information. Parkinsons Parkinson s PD. PD . (CDP setting Therefore studys (BESTest 2 (PD SitToStand Sit To Stand STS, , (STS) StepQuick Quick SQT, (SQT) (SUO Master platform 0.02 002 0 02 0.01 001 01 left 003 03 0.03 respectively, respectively respectively) 0007 007 0.007 0.001, 0001 0.001 0.001) 0.01. 0.002 0002 0.020. 0020 0.020 020 0.020) posturography information (STS (SQT 0.0 00 000 0.00 0.

https://doi.org/10.1016/j.clinsp.2024.100382

15.

The nutritional value of gliricidia in different fed forms: a systematic review forms
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Silva, Pedro Henrique Ferreira da

; Medeiros, Geovergue Rodrigues de

; Carvalho, Chrislanne Barreira de Macêdo

; Cavalcante, Iara Tamires Rodrigues

; Santos, Severino Guilherme Caetano Gonçalves dos

; Neves, Romildo da Silva

; Ribeiro, Neila Lidiany

; Costa, José Henrique Souza

; Sales-Silva, Tafnes Bernardo

Ciência Rural

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2024, Volume 54 Nº 11 elocation e20230475

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: Diferentes tipos de volumosos podem causar perdas na composição química e digestibilidade da gliricídia (Gliricidia sepium [Jacq.] Kunth ex Walp.) dependendo de sua confecção e do manejo da alimentação. O objetivo deste estudo foi sumarizar dados de trabalhos científicos com gliricídia e comparar seu valor nutritivo em diferentes formas de alimentação, sob uma abordagem de revisão sistemática e análise de componentes principais (ACP). Realizou-se um levantamento robusto de trabalhos científicos em web indexadores de periódicos, e em base de dados, de 1990 a 2023. Dados de 100 trabalhos foram sumarizados e analisados. Em 40% dos trabalhos, o valor nutricional da gliricídia foi determinado como folhas in natura, enquanto 30% foi na forma de ‘folhas + caules finos’, em 11% das oportunidades, a gliricídia foi avaliada na forma de silagem, seguido por 10% como farelo de folhas, e 9% como feno. Carboidratos não-fibrosos, proteína bruta (PB) e digestibilidade da matéria orgânica (MO) tiveram correlações negativas com fibra em detergente neutro (FDN), fibra em detergente ácido (FDA) e lignina. Os teores de FDN, FDA e PB foram capazes de explicar 71,00% da variação total dos dados no CP 1 e 2. Além disso, não houve discriminação entre as formas de alimentação, que tiveram composições semelhantes. Eventuais perdas de nutrientes relatadas em trabalhos científicos devido aos processos de ensilagem, fenação ou fabricação de farelo de folhas não descaracterizam o valor nutritivo da gliricídia. As diversas maneiras de confecção de volumosos e manejo da alimentação tradicionalmente utilizadas por agricultores são capazes de conservar a excelente qualidade nutricional da gliricídia. RESUMO Gliricidia Jacq. Jacq [Jacq. Walp. Walp ACP. ACP . (ACP) Realizouse Realizou se periódicos 199 2023 10 analisados 40 natura 30 finos, finos , finos’ 11 oportunidades silagem 9 feno nãofibrosos, nãofibrosos fibrosos, fibrosos não-fibrosos (PB MO (MO FDN (FDN) (FDA lignina 7100 71 00 71,00 2 disso semelhantes ensilagem [Jacq (ACP 19 202 4 3 (FDN 710 7 0 71,0 20 71,

ABSTRACT: Different types of supplied roughage may cause losses in the chemical composition and digestibility of gliricidia (Gliricidia sepium [Jacq.] Kunth ex Walp.) depending on their making and fed-out management. This study has summarized data from scientific studies on gliricidia and compared its nutritional value in various fed forms using a systematic review approach and principal component analysis (PCA). A robust survey of scientific papers was realized on web indexes of periodicals and databases from 1990 to 2023. Data from 100 scientific researches has been compiled and analyzed. In 40% of the trials, the gliricidia nutritional value was determined as fresh leaves, while 30% was in the form of ‘leaves + thin stems’. Gliricidia was evaluated as silage in 11% of the opportunities, followed by 10% as leaf meal and 9% as hay. Non-fiber carbohydrates, crude protein (CP), and organic matter digestibility (OM) had negative correlations with neutral detergent fiber (NDF), acid detergent fiber (ADF), and lignin. Only NDF, ADF, and CP contents could explain 71% of the total data variation (PC1 and PC2). There was no discrimination among fed forms, so they showed similar nutrient compositions. Eventual nutrient losses reported in the scientific researches owing to ensilage, haymaking, and leaf meal-making processes do not mischaracterize the gliricidia nutritional value. The assorted manners of roughage making, and fed-out management traditionally performed by farmers can conserve the excellent nutritional quality of gliricidia. ABSTRACT Jacq. Jacq [Jacq. Walp. Walp fedout out PCA. PCA . (PCA) 199 2023 10 analyzed 40 trials leaves 30 stems. stems stems’ 11 opportunities 9 hay Nonfiber Non carbohydrates CP, , (CP) OM (OM NDF (NDF) ADF (ADF) lignin 71 PC1 PC (PC PC2. PC2 PC2) compositions ensilage haymaking mealmaking [Jacq (PCA 19 202 1 4 3 (CP (NDF (ADF 7 20 2

https://doi.org/10.1590/0103-8478cr20230475

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