RESUMO: O peixe-boi amazônico (Trichechus inunguis) é um mamífero aquático ameaçado de extinção. Apesar disso, poucos estudos investigaram patógenos nessa espécie que podem impactar a saúde animal e humana. O objetivo deste estudo foi avaliar a frequência, distribuição e padrões de suscetibilidade antimicrobiana de Staphylococcus sp. e Escherichia coli pertencentes à microbiota nasal e retal de peixes-bois da Amazônia mantidos em cativeiro no Instituto Nacional de Pesquisas da Amazônia (INPA) (Amazonas, Brasil). Foram utilizados suabes retais e nasais de 44 peixes-boi de diferentes idades. O gênero Staphylococcus foi isolado do swab nasal de 32 (72,7%) animais, sendo que dois indivíduos apresentaram mais de uma espécie de Staphylococcus. S. sciuri foi a espécie mais comumente isolada. A resistência à penicilina foi observada em 13 (40,6%) isolados, sendo mais frequente do que os outros antimicrobianos testados (P = 0,01). Escherichia coli foi isolada dos suabes retais de todos os animais, sendo o filogrupo B1 o mais frequente entre as cepas obtidas (P = 0,0008). Quatro isolados (6,8%) foram positivos para fatores de virulência, três dos quais foram classificados como E. coli enterotoxigênica (ETEC) e um isolado como E. coli enteropatogênica (EPEC). Este é o primeiro estudo a avaliar Staphylococcus spp. e E. coli de amostras de peixe-boi da Amazônia. O estudo revelou colonização nasal por Staphylococcus sp., principalmente S. sciuri, e por isolados diarreiogênicos de E. coli, incluindo cepas resistentes a antimicrobianos.

ABSTRACT: The Amazonian manatee (Trichechusinunguis) is an aquatic mammal threatened with extinction. However, few studies have investigated the pathogens in this species, which may affect both animal and human health. This study aimed to evaluate the frequency, distribution, and patterns of antimicrobial susceptibility of Staphylococcus spp. and Escherichiacoli colonizing the nasal and rectal cavities of Amazonian manatees kept in captivity at the National Institute for Amazonian Research (INPA) in the state of Amazonas, Brazil. Rectal and nasal swabs from 44 manatees of different ages were used in this study. The genus Staphylococcus was isolated from the nasal swabs of 32 (72.7%) animals, with two individuals harboring more than one species of Staphylococcus. S. sciuri was the most commonly isolated species. Resistance to penicillin was observed in 13 (40.6%) isolates, more frequent than the other antimicrobials tested (P = 0.01). E. coli was isolated from the rectal swabs of all animals, with phylogroup B1 being the most frequent among the strains obtained (P = 0.0008). Four isolates (6.8%) were positive for virulence factors, three of which were classified as enterotoxigenicE. coli (ETEC) and one as enteropathogenicE. coli (EPEC). To our knowledge, this is the first study to evaluate Staphylococcus spp. and E. coli in Amazonian manatee samples. This study revealed nasal colonization by Staphylococcus spp., mainly S. sciuri, and diarrheagenicE. coli isolates, including antimicrobial-resistant strains.

RESUMO Introdução: a pandemia COVID-19 levou a um importante declínio mundial no volume cirúrgico devido ao adiamento de procedimentos eletivos. Este estudo avaliou o impacto da pandemia COVID-19 nos volumes e nos resultados da cirurgia abdominal em pacientes criticos. Métodos: pacientes internados para cuidados pósoperatórios foram avaliados retrospectivamente. Dados relativos aos desfechos perioperatórios foram comparados em dois períodos: janeiro-2017 a dezembro-2019 e janeiro-2020 a dezembro-2022, respectivamente, antes (período I) e depois (período II) da pandemia COVID-19. Resultados: foram investigados 1.402 pacientes (897 mulheres, idade 62+17 anos). A maioria dos pacientes foi submetida a cirurgia colorretal (n=393) e pancreatobiliar (n=240), sendo 52% dos procedimentos eletivos. O volume cirúrgico foi significativamente menor no período II (n=514) quando comparado ao período I (n=888). Não foi observada recuperação no número de procedimentos cirúrgicos em 2022 (n=135) quando comparado a 2021 (n=211) e 2020 (n=168). Indivíduos submetidos à cirurgia abdominal no período II apresentaram maior índice de comorbidade de Charlson (4,85+3,0 vs. 4,35+2,8, p=0,002), mais procedimentos emergenciais/urgentes (51% vs. 45%, p =0,03) e mais feridas potencialmente contaminadas (73,5% vs. 66,8%, p=0,02). Observou-se diminuição significativa no volume de cirurgia colorretal (24% vs, 31%, p<0,0001) após o início da pandemia de COVID-19, com 125 (8,9%) óbitos, nenhuma morte por COVID-19. A mortalidade foi maior no período II quando comparada ao período I (11% vs. 8%, p=0,08). Conclusões: a pandemia de COVID-19 foi associada à diminuição do volume cirúrgico de pacientes de alto risco sem recuperação aparente nos últimos anos. Nenhuma influência da COVID-19 foi observada na mortalidade pósoperatória.

ABSTRACT Backgrounds: COVID-19 pandemic led to a sharp decline in surgical volume worldwide due to the postponement of elective procedures. This study evaluated the impact of COVID-19 pandemic in surgical volumes and outcomes of abdominal surgery in high-risk patients requiring intensive care unit admission. Methods: patients admitted for postoperative care were retrospectively evaluated. Data concerning perioperative variables and outcomes were compared in two different periods: January 2017-December 2019 and January 2020-December 2022, respectively, before (period I) and after (period II) the onset of COVID-19 pandemic. Results: 1.402 patients (897 women, mean age 62+17 years) were investigated. Most of the patients underwent colorectal (n=393) and pancreato-biliary (n=240) surgery, 52% of elective procedures. Surgical volume was significantly lower in period II (n=514) when compared to period I (n= 888). No recovery was observed in the number of surgical procedures in 2022 (n=135) when compared to 2021(n=211) and 2020 (n=168). Subjects who underwent abdominal surgery in period II had higher Charlson comorbidity index (4,85+3,0 vs. 4,35+2,8, p=0,002), more emergent/urgent procedures (51% vs. 45%, p=0,03) and more clean-contaminated wounds (73,5% vs. 66,8%, p=0,02). A significant decrease in the volume of colorectal surgery was also observed (24% vs, 31%, p<0,0001) after the onset of COVID-19 pandemic, 125 (8,9%) died, no deaths due to COVID-19 infection. Mortality was higher in period II when compared to period I (11% vs. 8%, p=0,08). Conclusions: COVID-19 pandemic was associated with a decrease in surgical volume of high-risk patients without apparent recovery in recent years. No influence of COVID-19 was noted in postoperative mortality.

Resumo Lajes com fôrmas metálicas utilizadas durante a concretagem são um sistema estrutural misto composto de aço e concreto. O modo de falha por cisalhamento horizontal é o mais comum em lajes mistas com bases em numerosos estudos experimentais. Esta falha consiste na perda de aderência e contato mecânico do sistema misto e, frequentemente, ocorre antes do sistema atingir sua capacidade máxima à flexão plástica. Assim, o projeto deste sistema construtivo é determinado pela sua resistência ao cisalhamento longitudinal, que pode ser determinada pelo método semiempírico convencional m-k com base no ensaio de flexão de quatro pontos proposto por diversas normas. Um dos fatores conhecidos de maior influência na resistência ao cisalhamento longitudinal é a geometria das mossas presentes na fôrma metálica. Simulações computacionais por meio do método dos elementos finitos calibradas com base em resultados experimentais podem ser uma alternativa à realização deste tipo de ensaio. O presente trabalho modificou e adaptou uma metodologia que inclui explicitamente a geometria das mossas na análise de modo a usar materiais e produtos com características disponíveis para os engenheiros estruturais do Brasil e usou esta metodologia para derivar coeficientes m-k. Isto foi feito com o objetivo de simular numericamente o efeito de parâmetros relevantes para determinar a resistência ao cisalhamento longitudinal na interface aço-concreto de lajes mistas. Gráficos de força versus deslocamentos foram obtidos e os valores dos coeficientes m-k foram comparados com aqueles de outros autores na literatura. As simulações apresentaram valores consistentes com a literatura e, em relação ao modo de ruptura por cisalhamento longitudinal, as simulações numéricas realizadas apresentaram resistências últimas com bom ajuste medido pelo coeficiente de determinação convencional chegando a um valor bom para a relação semiempírica utilizando os resultados dos dados de elementos finitos.

Abstract Slabs with cast-in-place steel formwork are composite structural elements composed of concrete and steel. The horizontal shear failure mode is the most common in composite slabs based on numerous experimental studies. This failure mode consists of loss of adherence and mechanical contact at the interface of the composite system and often occurs before the system reaches its full bending capacity. Thus, the design of this constructive system is determined by the value of the longitudinal shear strength, which can be computed by the semi-empirical so-called traditional m-k method based on the four-point bending test proposed by several standards. One of the most known factors to influence the longitudinal shear strength is the geometry of embossments present in the steel work. Calibrated computational simulation using the finite element method can be an alternative way to simulate this type of test. The present work modified and adapted a methodology explicitly including the geometry of embossments in the analysis in order to use materials and products with characteristics available to structural engineers in Brazil and used this methodology to derive m-k coefficients. This is being made with the objective of numerically simulating the effect of relevant parameters for determining the longitudinal shear strength at the concrete-steel interface of composite slabs. Force versus slip, force versus displacement graphs were obtained and the m-k coefficient values found were compared with those of other authors in the literature. The simulations presented values consistent with the literature and in relation to the rupture mode by longitudinal shear, the numerical simulations performed presented ultimate strengths with goodness-of-fit measured by the conventional coefficient of determination, reaching a good value for the semi-empirical relation using the results of the finite element data.

RESUMO Objetivo: analisar a produção do cuidado, no que tange ao acolhimento, vínculo, resolubilidade e responsabilização, às pessoas indígenas com tuberculose. Método: estudo qualitativo, com observação sistemática, análise documental e entrevista semiestruturada com trabalhadores de saúde e gestão da saúde indígena e secretaria municipal de saúde de Banzaê/Bahia - Brazil. Realizou-se a triangulação de dados das entrevistas, registros documentais e observação do campo. Resultados: o acolhimento é dado quando se reconhece a necessidade de compreensão e respeito às especificidades culturais, o vínculo está relacionado à cultura do trabalhador de saúde, a resolubilidade é dificultada em virtude da gestão municipal e há presença de divergências entre os responsáveis pela saúde indígena na responsabilização pelo cuidado. Conclusão: são necessárias condutas coletivas entre as instâncias municipal, estadual e os Distritos Sanitários Especiais Indígenas, quanto ao percurso dos serviços de saúde da rede. Objetivo cuidado tuberculose Método qualitativo sistemática BanzaêBahia Banzaê Bahia Brazil Realizouse Realizou entrevistas campo Resultados culturais Conclusão Indígenas rede

RESUMEN Objetivo: analizar la producción del cuidado, en lo que respecta a la acogida, el vínculo, la resolubilidad y la responsabilización, a las personas indígenas con tuberculosis. Método: estudio cualitativo, con observación sistemática, análisis documental y entrevista semiestructurada con trabajadores de salud y gestión de la salud indígena y secretaría municipal de salud de Banzaê/Bahia - Brasil. Se realizó la triangulación de datos de las entrevistas, registros documentales y observación del campo. Resultados: la acogida se da cuando se reconoce la necesidad de comprensión y respeto a las especificidades culturales, el vínculo está relacionado con la cultura del trabajador de salud, la resolubilidad se ve dificultada debido a la gestión municipal y hay presencia de divergencias entre los responsables de la salud indígena en la responsabilización del cuidado. Conclusión: son necesarias conductas colectivas entre las instancias municipal, estatal y los Distritos Sanitarios Especiales Indígenas, en cuanto al recorrido de los servicios de salud de la red. Objetivo cuidado tuberculosis Método cualitativo sistemática BanzaêBahia Banzaê Bahia Brasil entrevistas campo Resultados culturales Conclusión Indígenas red

ABSTRACT Objective: To analyze the production of care, regarding reception, bonding, resolvability, and accountability, for indigenous people with tuberculosis. Method: Qualitative study, with systematic observation, document analysis, and semi-structured interviews with health workers and indigenous health management and the municipal health department of Banzaê/Bahia - Brazil. Data triangulation was carried out from interviews, documentary records, and field observation. Results: The reception is given when the need for understanding and respect for cultural specificities is recognized, the bond is related to the health worker’s culture, the resolvability is hindered due to municipal management, and there are divergences among those responsible for indigenous health in taking responsibility for care. Conclusion: Collective actions are necessary between the municipal, state instances and the Special Indigenous Sanitary Districts, regarding the path of the health services network. Objective care bonding accountability tuberculosis Method study observation analysis semistructured semi structured BanzaêBahia Banzaê Bahia Brazil records Results recognized worker s culture Conclusion Districts network

ABSTRACT: The present study proposed to describe, through a literature review, the use of new therapeutic management which allows for offering a better quality of life to individuals affected by these pathologies. A bibliographic search was conducted in the main health databases PUBMED (www.pubmed.gov) and Scholar Google (www.scholar.google.com.br), in which studies published from 1987 to 2023 were collected. In the first stage, the list of retrieved articles was examined by reading the titles and abstracts. In the second stage, the studies were selected by reading the full contents. Two authors (JDMM and DAQ) performed stages 1 and 2. Experimental, clinical, case-control, randomized controlled, and laboratory cohort studies, case reports, systematic reviews, and literature reviews, which were developed in living individuals, were included. Therefore, articles that did not deal with the subject in question, letters to the editor, opinion articles, duplicated literature in databases, and literature that did not address the variables under study, we re excluded. Contemporary dentistry uses alternative treatments capable of improving the patient's condition since a cure is not always possible. Therefore, the possibility of improving the quality of life becomes an important point to be reached. Evidence-based healthcare has made great advances in recent decades, especially in the areas of orofacial pain, TMD, and occlusion, especially related to orthodontic, prosthetic, and restorative care.

RESUMEN: En el presente estudio se propuso describir, a través de una revisión bibliográfica, el uso de nuevos manejos terapéuticos que permitan brindar una mejor calidad de vida a los individuos afectados por estas patologías. Se realizó una búsqueda bibliográfica en las principales bases de datos de salud PUBMED (www.pubmed.gov) y Scholar Google (www.scholar.google.com.br), en las que se recopilaron estudios publicados entre 1987 y 2023. En la primera etapa, se examinó la lista de artículos recuperados mediante la lectura de los títulos y resúmenes. En la segunda etapa, los estudios fueron seleccionados mediante la lectura del contenido completo. Dos autores (JDMM y DAQ) realizaron las etapas 1 y 2. Se incluyeron estudios de cohortes experimentales, clínicos, de casos y controles, controlados aleatorios y de laboratorio, informes de casos, revisiones sistemáticas y revisiones de la literatura, que se desarrollaron en individuos vivos. Por lo tanto, se excluyeron artículos que no trataran el tema en cuestión, cartas al editor, artículos de opinión, literatura duplicada en bases de datos y literatura que no abordara las variables en estudio. La odontología contemporánea utiliza tratamientos alternativos capaces de mejorar el estado del paciente, ya que no siempre es posible la curación. Por lo tanto, la posibilidad de mejorar la calidad de vida se convierte en un objetivo importante. La atención médica basada en la evidencia ha logrado grandes avances en las últimas décadas, especialmente en las áreas de dolor orofacial, TMD y oclusión, especialmente en relación con la atención de ortodoncia, prótesis y restauración.

ABSTRACT OBJECTIVE To evaluate the quality of information on gestational syphilis (GS) and congenital syphilis (CS) on the Sistema de Informação de Agravos de Notificação (SINAN-Syphilis Brazil – Notifiable Diseases Information System) by compiling and validating completeness indicators between 2007 and 2018. METHODS Overall, care, and socioeconomic completeness scores were compiled based on selected variables, by using ad hoc weights assigned by experts. The completeness scores were analysed, considering the region and area of residence, the pregnant woman’s race/colour, and the year of case notification. Pearson’s correlation coefficients were used to validate the scores obtained by the weighted average method, compared with the values obtained by principal component analysis (PCA). RESULTS Most selected variables presented a good or excellent degree of completeness for GS and CS, except for clinical classification, pregnant woman’s level of education, partner’s treatment, and child’s race/colour, which were classified as poor or very poor. The overall (89.93% versus 89.69%) and socioeconomic (88.71% versus 88.24%) completeness scores for GS and CS, respectively, were classified as regular, whereas the care score (GS-90.88%, and CS-90.72%) was good, despite improvements over time. Differences in the overall, care and socioeconomic completeness scores according to region, area of residence, and ethnic-racial groups were reported for syphilis notifications. The completeness scores estimated by the weighted average method and PCA showed a strong linear correlation (> 0.90). CONCLUSION The completeness of GS and CS notifications has been improving in recent years, highlighting the variables that form the care score, compared with the socioeconomic scores, despite differences between regions, area of residence, and ethnic-racial groups. The weighted average was a viable methodological alternative easily operationalised to estimate data completeness scores, allowing routine monitoring of the completeness of gestational and congenital syphilis records.

ABSTRACT The Cerambycidae Oncideres impluviata (Germar, 1823) is an important insect pest for Acacia mearnsii De Wild in Southern Brazil. The damage caused by their girdling behavior reduces tree productivity, specially in the early years of plant establishment, when girdling is performed on the main trunk of trees. Here, we used a fragment of the mtDNA COI gene to analyze the genetic diversity, population structure and demography of O. impluviata in Southern Brazil, as well as to present the first hypothesis of phylogenetic relationships among species of the genus Oncideres. Our results identified five distinct haplotypes among the populations of O. impluviata, with the most common haplotype identified as O.imp_COI_01. The phylogenetic inferences corroborated the monophyly of O. impluviata with maximum statistical support. In addition, the phylogeny recovered three main population strains that are largely congruent with the haplotype network, which includes two lineages that are found in different edaphic regions of Rio Grande do Sul (Serra do Sudeste and Encosta Inferior do Nordeste). This is the first molecular phylogenetic assessment of O. impluviata. Our findings provide insights into the evolution of a significant species for the Brazilian forestry sector, as well as new resources for planning of pest management strategies. Germar, Germar (Germar 1823 Brazil productivity establishment trees Here diversity O OimpCOI01 OimpCOI imp 01 O.imp_COI_01 support addition network Serra Nordeste. Nordeste . Nordeste) sector strategies 182 OimpCOI0 0 O.imp_COI_0 18 O.imp_COI_ 1 O.imp_COI

Resumen Durante la década de 1960, período de grandes cambios económicos y sociales en Brasil, la Folkcomunicación nació con el objetivo de suplir problemas de la Comunicación, a partir de la observación y comprensión de las reacciones de un pueblo que vivía al margen, y sin compromiso con los medios de comunicación. En el mismo período, la “comunicación” y el “desarrollo”, buscó un clima público favorable a la transformación social en América Latina. Por considerar que los dos enfoques teóricos se complementan, el presente trabajo propone un cruce entre Folkcomunicación y Comunicación para el Desarrollo (C4D), a partir de una revisión bibliográfica. La contribución de esta investigación se basa en el reconocimiento de la Folkcomunicación desde la perspectiva del desarrollo social y en la valorización del diálogo entre las dos vertientes. 1960 Brasil margen comunicación “comunicación desarrollo, , “desarrollo” Latina complementan C4D, C4D CD C D (C4D) bibliográfica vertientes 196 “desarrollo (C4D 19 1

Resumo Pensada na década de 1960, período de grandes transformações econômicas e sociais no Brasil, a Folkcomunicação nasce com o objetivo de suprir os problemas da Comunicação ao enxergar e compreender as reações de um povo que vivia à margem, sem engajamento com os meios de comunicação. Neste mesmo período, a acoplagem “comunicação” e “desenvolvimento” busca uma atmosfera pública favorável à transformação social na América Latina. Por considerar que as duas abordagens teóricas se complementam, o presente trabalho propõe um cruzamento entre a Folkcomunicação e a Comunicação para o Desenvolvimento (C4D), a partir de revisão bibliográfica. A contribuição desta pesquisa se baseia no reconhecimento da Folkcomunicação dentro da perspectiva do desenvolvimento social e na valorização do diálogo entre as duas vertentes. 1960 Brasil margem comunicação “comunicação “desenvolvimento Latina complementam C4D, C4D CD , C D (C4D) bibliográfica vertentes 196 (C4D 19 1

Abstract Conceived in the 1960s, a period of great economic and social transformations in Brazil, Folkcommunication was born with the aim of overcoming Communication problems by seeing and understanding the reactions of a people who lived on the margins, without any engagement with the media. In this same period, the coupling of “communication” and “development” seeks a public atmosphere favorable to social transformation in Latin America. Considering that the two theoretical approaches complement each other, this work proposes a crossing between Folkcommunication and Communication for Development (C4D), based on a literature review. The contribution of this research is based on the recognition of folkcommunication within the perspective of social development and on the appreciation of the dialogue between the two aspects. 1960s s Brazil margins media communication “communication “development America other C4D, C4D CD , C D (C4D) review aspects (C4D

Abstract Hydrocotyle umbellata L., Araliaceae, is a species that is recommended in Ayurvedic medicine for its effects on the central nervous system, such as anxiolytic and memory-stimulant effects. Despite the medicinal potential of this species, its phytopharmaceutical and technological potential to produce standardized extracts has not been investigated. This study analyzes the influence of spray drying parameters on the contents of the chemical markers (total phenolic, total flavonoid, and hibalactone) and the functional properties of H. umbellata extract. The optimization of drying conditions was performed using a central composite design combined with response surface methodology and desirability function approach. The mathematical models fitted to experimental data indicated that all the evaluated drying parameters significantly influenced the chemical contents. The optimal conditions were: inlet temperature of 120 °C, feed flow rate of 4 mL min-1, and colloidal silicon dioxide:maltodextrin ratio of 16%:4%. Under these conditions, the powder samples had spherical particles and desirable physicochemical and functional properties, such as low water activity and moisture content, good product recovery, reconstitution, and flowability. Thus, spray drying might be a promising technique for processing standardized H. umbellata extracts. L L. Araliaceae system memorystimulant memory stimulant investigated phenolic flavonoid hibalactone H extract approach were 12 C °C min1, min1 min 1, 1 min-1 dioxidemaltodextrin dioxide maltodextrin 16%4%. 164 16% 4%. 16 16%:4% content recovery reconstitution flowability Thus min- 16%4% 4% 16%:4 16%4 16%:

Abstract Inborn errors of metabolism are rare disorders with few therapeutic options for their treatments, which can make patients suffer with complications. Therefore, compounded drugs might be a promising option given that they have the ability of meeting the patient’s specific needs, (i) identification of the main drugs described in the literature; (ii) proposal of compounding systems and (iii) calculation of the budgetary addition for the inclusion of these drugs into the Brazilian Unified Health System. The research conducted a literature review and used management data as well as data obtained from official Federal District government websites. The study identified 31 drugs for the treatment of inborn errors of metabolism. Fifty eight percent (58%) (18) of the medicines had their current demand identified, which are currently unmet by the local Health System. The estimated budget for the production of compounded drugs was of R$363,16.98 per year for approximately 300 patients. This estimated cost represents a budgetary addition of only 0.17% from the total of expenditures planned for drug acquirement. There is a therapeutic gap for inborn errors of metabolism and compounding pharmacies show potential in ensuring access to medicine therapy with a low-cost investment. treatments complications Therefore patient s needs i (i ii (ii iii (iii System websites 3 58% 58 (58% 18 (18 R3631698 R 363 16 98 R$363,16.9 30 017 0 17 0.17 acquirement lowcost low investment 5 (58 1 (1 R363169 36 9 R$363,16. 01 0.1 (5 ( R36316 R$363,16 0. R3631 R$363,1 R363 R$363, R36 R$363 R3 R$36 R$3 R$

ABSTRACT COVID-19 is commonly associated with high serum levels of pro-inflammatory cytokines, and the post-infection status can disturb self-tolerance and trigger autoimmune responses. We are reporting a 45-year-old male who was admitted with fatigue, jaundice, elevated liver enzymes (with cholestatic pattern), and acute kidney injury two weeks after recovering from a mild SARS-CoV-2 infection. Serologies for viral hepatitis and anti-mitochondrial antibody were negative, while anti-nuclear and anti-smooth muscle antibodies were positive. There were no signs of chronic liver disease, and a magnetic resonance cholangiography showed no dilatation of biliary ducts. Histologic evaluation of the liver evidenced numerous foci of lobular necrosis without ductopenia or portal biliary reaction. Considering the autoantibody profile and histologic changes, the medical team started oral prednisone, but there was a suboptimal biochemical response in the outpatient follow-up. Two months later, a second liver biopsy was performed and revealed non-suppurative destructive chronic cholangitis, extensive areas of confluent necrosis with hepatocytes regenerating into pseudorosettes, and numerous plasma cells. According to the Paris Criteria, the patient was then diagnosed with an autoimmune hepatitis-primary biliary cholangitis overlap syndrome (AIH-PBC-OS). After adding azathioprine and ursodeoxycholic acid to the treatment, there was a satisfactory response. This is the second worldwide report of an AIH-PBC-OS triggered by COVID-19, but the first case with a negative anti-mitochondrial antibody. In this setting, histologic evaluation of the liver by an experienced pathologist is a hallmark of achieving the diagnosis and correctly treat the patient. COVID19 COVID 19 COVID-1 proinflammatory pro inflammatory cytokines postinfection post infection selftolerance self tolerance responses 45yearold yearold 45 year old fatigue jaundice pattern, pattern , pattern) SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- antimitochondrial anti mitochondrial antinuclear nuclear antismooth smooth positive disease ducts reaction changes prednisone followup. followup follow up. up follow-up later nonsuppurative non suppurative pseudorosettes cells Criteria hepatitisprimary primary AIHPBCOS. AIHPBCOS AIH PBC OS . (AIH-PBC-OS) treatment COVID19, 19, setting COVID1 1 COVID- 4 SARS-CoV (AIH-PBC-OS

ABSTRACT OBJECTIVE To evaluate the quality of information on gestational syphilis (GS) and congenital syphilis (CS) on the Sistema de Informação de Agravos de Notificação (SINAN-Syphilis Brazil – Notifiable Diseases Information System) by compiling and validating completeness indicators between 2007 and 2018. METHODS Overall, care, and socioeconomic completeness scores were compiled based on selected variables, by using ad hoc weights assigned by experts. The completeness scores were analysed, considering the region and area of residence, the pregnant woman’s race/colour, and the year of case notification. Pearson’s correlation coefficients were used to validate the scores obtained by the weighted average method, compared with the values obtained by principal component analysis (PCA). RESULTS Most selected variables presented a good or excellent degree of completeness for GS and CS, except for clinical classification, pregnant woman’s level of education, partner’s treatment, and child’s race/colour, which were classified as poor or very poor. The overall (89.93% versus 89.69%) and socioeconomic (88.71% versus 88.24%) completeness scores for GS and CS, respectively, were classified as regular, whereas the care score (GS-90.88%, and CS-90.72%) was good, despite improvements over time. Differences in the overall, care and socioeconomic completeness scores according to region, area of residence, and ethnic-racial groups were reported for syphilis notifications. The completeness scores estimated by the weighted average method and PCA showed a strong linear correlation (> 0.90). CONCLUSION The completeness of GS and CS notifications has been improving in recent years, highlighting the variables that form the care score, compared with the socioeconomic scores, despite differences between regions, area of residence, and ethnic-racial groups. The weighted average was a viable methodological alternative easily operationalised to estimate data completeness scores, allowing routine monitoring of the completeness of gestational and congenital syphilis records. (GS (CS SINANSyphilis SINAN Syphilis System 200 2018 Overall experts analysed residence womans woman s racecolour race colour race/colour notification Pearsons Pearson PCA. . (PCA) classification education partners partner treatment childs child 89.93% 8993 89 93 (89.93 89.69% 8969 69 88.71% 8871 88 71 (88.71 88.24% 8824 24 respectively regular GS90.88%, GS9088 90.88%, 90 (GS-90.88% CS90.72% CS9072 90.72% 72 CS-90.72% time ethnicracial ethnic racial > ( 0.90. 090 0.90 0 0.90) years regions records 20 201 (PCA 89.93 899 8 9 (89.9 89.69 896 6 88.71 887 7 (88.7 88.24 882 2 GS90 GS90.88% GS908 9088 90.88% (GS-90.88 CS90 CS90.72 CS907 9072 90.72 CS-90.72 09 0.9 89.9 (89. 89.6 88.7 (88. 88.2 GS9 GS90.88 908 90.88 (GS-90.8 CS9 CS90.7 907 90.7 CS-90.7 0. 89. (89 88. (88 GS90.8 90.8 (GS-90. CS90. 90. CS-90. (8 GS90. (GS-90 CS-90 (GS-9 CS-9 (GS- CS-

RESUMO Tanto a osteoartrite quanto a infecção pelo parvovírus B19 apresentam evolução muitas vezes silenciosa e gradual, uma vez que a primeira é um processo degenerativo, e a segunda é geralmente assintomática, podendo persistir no corpo do indivíduo por toda a sua vida. Esta revisão bibliográfica visa analisar estudos clínicos que estabeleceram a correlação entre a osteoartrite degenerativa e a infecção pelo parvovírus B19. Dos 62 artigos encontrados, foram eleitos 25 para leitura em sua totalidade. Analisando apenas os artigos que estabelecem a relação entre as patologias, temos sete que confirmaram essa relação, enquanto um deles afirmou que não há relação. Não houve correlação objetiva entre os demais artigos estudados. Nossos resultados sugerem que há estreita relação entre a osteoartrite e o parvovírus B19, que tende a ser uma doença de causa adquirida com maior prevalência em mulheres e idosos, porém que pode se manifestar durante toda a vida. Contudo, é crucial a realização de novos estudos envolvendo antecedentes familiares de pacientes com osteoartrite e com positividade para o parvovírus B19 e estudos de coorte entre a infância e o adulto-idoso, a fim de elucidar essa dualidade de causa congênita-adquirida e, enfim, levantar alternativas de tratamento. Nível de Evidência II, Revisão Sistemática de Estudos de Nível II. B B1 gradual degenerativo assintomática vida 6 encontrados 2 totalidade patologias estudados idosos Contudo adultoidoso, adultoidoso adulto idoso, idoso adulto-idoso congênitaadquirida congênita enfim tratamento II

ABSTRACT Osteoarthritis and Parvovirus B19 infection present silent and gradual evolution, since the former is a degenerative process while the latter is often asymptomatic and may persist in the individual’s body during their life. This study aims to analyze clinical studies that establish a correlation between degenerative osteoarthritis and Parvovirus B19 infection. Of the 62 studies found, 25 were chosen for reading in full. Analyzing only the studies that establish the correlation between the pathologies, seven confirm this relationship between Parvovirus B19 and Osteoarthritis, while one reports no relationship. No objective correlation could be found between the other articles studied. Our findings suggest that there is a close relationship between Parvovirus B19 and Osteoarthritis, with a higher prevalence of acquired causes, women and older adults, but it can manifest during life. However, it is essential to carry out new studies involving family history of patients with Osteoarthritis with positivity of Parvovirus B19, cohort studies between childhood and adult-old adult, so that it can elucidate this duality of congenital-acquired cause and, finally, raise treatment alternatives. Level of Evidence II, Systematic Review of Level II Studies. B B1 evolution individuals individual s life 6 2 full pathologies studied causes adults However adultold adult old congenitalacquired congenital finally alternatives Studies

RESUMO Objetivo Avaliar os efeitos da restrição proteica materna sobre o estresse oxidativo no hipotálamo de ratos de 60 dias de idade. Métodos Ratos Wistar machos foram divididos em dois grupos experimentais de acordo com a dieta da mãe durante a gestação e lactação: grupo controle (NP: 17% caseína n=6) e grupo desnutrido (LP: 8% caseína n=6). Aos 60 dias de vida, os ratos foram sacrificados para coleta do hipotálamo para posterior análise bioquímica. Resultados Os resultados demonstraram aumento do estresse oxidativo no grupo desnutrido, observado através do aumento do conteúdo de cabonilas (p=0,0357) e redução da atividade da enzima glutationa-S-transferase (p=0,0257) e da capacidade antioxidante não enzimática, evidenciada pela queda da razão glutationa reduzida/glutationa oxidada (p=0,0406) e dos níveis de tióis totais (p=0,0166). Conclusão Uma dieta com baixo teor de proteínas durante a gestação e lactação está intimamente associada ao aumento do estresse oxidativo e à redução da capacidade antioxidante no hipotálamo de ratos de 60 dias de vida. 6 idade NP (NP 17 n=6 n6 n LP (LP 8 n=6. . vida bioquímica p=0,0357 p00357 p 0 0357 (p=0,0357 glutationaStransferase S transferase p=0,0257 p00257 0257 (p=0,0257 enzimática reduzidaglutationa reduzida p=0,0406 p00406 0406 (p=0,0406 p=0,0166. p00166 p=0,0166 0166 (p=0,0166) 1 n= p=0,035 p0035 035 (p=0,035 p=0,025 p0025 025 (p=0,025 p=0,040 p0040 040 (p=0,040 p0016 p=0,016 016 (p=0,0166 p=0,03 p003 03 (p=0,03 p=0,02 p002 02 (p=0,02 p=0,04 p004 04 (p=0,04 p001 p=0,01 01 (p=0,016 p=0,0 p00 (p=0,0 (p=0,01 p=0, p0 (p=0, p=0 (p=0 p= (p= (p

ABSTRACT Objective Evaluate the effects of maternal low-protein diet on the oxidative stress in the hypothalamus of 60-day-old rats. Methods Male Wistar rats were divided into two experimental groups according to the mother’s diet during pregnancy and lactation; control group (NP:17% casein n=6) and a malnourished group (LP:8% casein n=6). At 60 days of life, the rats were sacrificed for the collection of the hypothalamus for further biochemical analysis. Results Our results showed an increase in oxidative stress in malnourished group, observed through an increase in carbonyl content (p=0.0357), a reduction in the activity of the glutathione-S-transferase enzyme (p=0.0257), and a reduction in the non-enzymatic antioxidant capacity evidenced by the decrease in the ratio reduced glutathione/oxidized glutathione (p=0.0406) and total thiol levels (p=0.0166). Conclusion A low-protein diet during pregnancy and lactation is closely associated with increased oxidative stress and reduced antioxidant capacity in the hypothalamus of sixty-day-old rats. lowprotein low protein 60dayold dayold day old mothers mother s NP17% NP17 NP 17% 17 (NP:17 n=6 n6 n 6 LP8% LP8 LP 8% 8 (LP:8 n=6. . life analysis p=0.0357, p00357 p p=0.0357 , 0 0357 (p=0.0357) glutathioneStransferase S transferase p=0.0257, p00257 p=0.0257 0257 (p=0.0257) nonenzymatic non enzymatic glutathioneoxidized oxidized p=0.0406 p00406 0406 (p=0.0406 p=0.0166. p00166 p=0.0166 0166 (p=0.0166) sixtydayold sixty NP1 1 (NP:1 n= (LP: p0035 p=0.035 035 (p=0.0357 p0025 p=0.025 025 (p=0.0257 p=0.040 p0040 040 (p=0.040 p0016 p=0.016 016 (p=0.0166 (NP: (LP p003 p=0.03 03 (p=0.035 p002 p=0.02 02 (p=0.025 p=0.04 p004 04 (p=0.04 p001 p=0.01 01 (p=0.016 (NP p00 p=0.0 (p=0.03 (p=0.02 (p=0.0 (p=0.01 p0 p=0. (p=0. p=0 (p=0 p= (p= (p

RESUMEN Objetivo: Estudiar el impacto de la pandemia de COVID-19 en las cirugías de columna en un Centro de Referencia para Urgencias de Alta Complejidad en Goiás. Métodos: Se realizó un estudio observacional retrospectivo a partir de datos de historias clínicas de pacientes operados de la columna entre septiembre de 2017 y septiembre de 2021. Los voluntarios se dividieron en dos grupos: antes de la pandemia y durante la pandemia, considerando abril de 2020 como punto de partida. De las historias clínicas se extrajo: edad, sexo, escolaridad, etiología, déficit neurológico, región de la columna abordada, mecanismo del trauma, vértebras fracturadas, tipo de fractura, tiempo de estancia en la UCI, test COVID (PCR) y fallecidos. Se utilizaron las pruebas estadísticas t de Student, chi-cuadrado y exacta de Fisher para comparar el perfil pre y postpandemia. Además, se aplicó la prueba de correlación de Spearman para verificar la correlación entre las variables, considerando p<0,05. Resultados: se analizaron 388 historias clínicas, mostrando un aumento del 15% en las cirugías de columna durante la pandemia. Hubo una diferencia significativa en la etiología de las cirugías (p=0,05), siendo más frecuente el traumatismo lumbar en los hombres y también más casos de déficit neurológico (p=0,001). También hubo una reducción en la estancia en la UTI (p=0,0001), que fue menor durante la pandemia. Conclusión: La pandemia de COVID-19 no impactó directamente en el número de cirugías realizadas en un Hospital de Referencia en Urgencias de Alta Complejidad en Goiás, pero hubo una priorización de las cirugías de emergencia. Nivel de Evidencia II; Estudio Retrospectivo. Objetivo COVID19 19 COVID-1 Goiás Métodos 201 2021 grupos 202 partida extrajo edad sexo escolaridad abordada trauma fracturadas fractura UCI PCR (PCR fallecidos Student chicuadrado chi cuadrado postpandemia Además variables p005 p 0 05 p<0,05 Resultados 38 15 p=0,05, p=0,05 , (p=0,05) p=0,001. p0001 p=0,001 . 001 (p=0,001) p=0,0001, p00001 p=0,0001 0001 (p=0,0001) Conclusión emergencia II Retrospectivo COVID1 1 COVID- 20 p00 p<0,0 3 p=0,0 (p=0,05 p000 p=0,00 00 (p=0,001 p0000 p=0,000 000 (p=0,0001 2 p0 p<0, p=0, (p=0,0 (p=0,00 (p=0,000 p<0 p=0 (p=0, p< p= (p=0 (p= (p

RESUMO Objetivo: Estudar o impacto da pandemia de COVID-19 nas cirurgias de coluna em um Centro de Referência em Urgências de Alta Complexidade em Goiás. Métodos: Foi realizado um estudo retrospectivo, observacional a partir de dados de prontuários médicos de pacientes submetidos à cirurgia de coluna entre setembro de 2017 a setembro de 2021. Os voluntários foram divididos em dois grupos: antes da pandemia e durante a pandemia, considerando abril de 2020 como marco de início. Foram extraídos dos prontuários: idade, gênero, escolaridade, etiologia, déficit neurológico, região da coluna abordada, mecanismo de trauma, vértebras fraturadas, tipo de fratura, tempo de internação em UTI, teste de COVID (PCR) e óbitos. Utilizou-se os testes estatísticos T de Student, Qui quadrado e exato de Fisher para realizar a comparação entre o perfil pré e pós-pandemia. Além disso, foi aplicado o teste de correlação de Spearman para verificar a correlação entre as variáveis, considerando p<0,05. Resultados: Foram analisados 388 prontuários, constatando um aumento de 15% nas cirurgias de coluna durante a pandemia. Houve diferença significativa na etiologia das cirurgias (p=0,05), sendo mais prevalentes traumas lombares em homens e também mais casos de déficits neurológicos (p=0,001). Notou-se também uma redução no tempo de internação na UTI (p=0,0001), que foi menor durante a pandemia. Conclusão: A pandemia de COVID-19 não impactou diretamente na quantidade de cirurgias realizadas em um Hospital de Referência em Urgências de Alta Complexidade em Goiás, mas verificou-se uma priorização das cirurgias emergenciais. Nível de Evidência II; Estudo Retrospectivo. Objetivo COVID19 19 COVID-1 Goiás Métodos retrospectivo 201 2021 grupos 202 início idade gênero escolaridade neurológico abordada trauma fraturadas fratura PCR (PCR óbitos Utilizouse Utilizou se Student póspandemia. póspandemia pós pós-pandemia disso variáveis p005 p 0 05 p<0,05 Resultados 38 15 p=0,05, p=0,05 , (p=0,05) p=0,001. p0001 p=0,001 . 001 (p=0,001) Notouse Notou p=0,0001, p00001 p=0,0001 0001 (p=0,0001) Conclusão verificouse verificou emergenciais II Retrospectivo COVID1 1 COVID- 20 p00 p<0,0 3 p=0,0 (p=0,05 p000 p=0,00 00 (p=0,001 p0000 p=0,000 000 (p=0,0001 2 p0 p<0, p=0, (p=0,0 (p=0,00 (p=0,000 p<0 p=0 (p=0, p< p= (p=0 (p= (p

ABSTRACT Objective: To study the impact of the COVID-19 pandemic on spine surgeries at a Reference Center for High Complexity Urgencies in Goiás. Methods: A retrospective, observational study was carried out based on data from medical records of patients undergoing spine surgery between September 2017 and September 2021. Volunteers were divided into two groups: before and during the pandemic, considering April 2020 as a starting point. The following was extracted from the medical records: age, gender, education, etiology, neurological deficit, region of the spine addressed, mechanism of trauma, fractured vertebrae, type of fracture, length of stay in the ICU, COVID test (PCR), and deaths. Student t, chi-square, and Fisher’s exact statistical tests were used to compare the preand post-pandemic profiles. In addition, Spearman’s correlation test was applied to verify the correlation between variables, considering p<0.05. Results: 388 medical records were analyzed, showing a 15% increase in spine surgeries during the pandemic. There was a significant difference in the etiology of surgeries (p=0.05), with lumbar trauma being more prevalent in men and also more cases of neurological deficits (p=0.001). There was also a reduction in the length of stay in the ICU (p=0.0001), which was lower during the pandemic. Conclusion: The COVID-19 pandemic did not directly impact the number of surgeries performed at a Reference Hospital in High Complexity Urgencies in Goiás, but there was a prioritization of emergency surgeries. Level of Evidence II; Retrospective Study. Objective COVID19 19 COVID-1 Goiás Methods retrospective 201 2021 groups 202 point age gender education deficit addressed vertebrae fracture PCR, PCR , (PCR) deaths t chisquare, chisquare chi square, square chi-square Fishers Fisher s postpandemic post profiles addition Spearmans Spearman variables p005 p 0 05 p<0.05 Results 38 analyzed 15 p=0.05, p=0.05 (p=0.05) p=0.001. p0001 p=0.001 . 001 (p=0.001) p=0.0001, p00001 p=0.0001 0001 (p=0.0001) Conclusion II Study COVID1 1 COVID- 20 (PCR p00 p<0.0 3 p=0.0 (p=0.05 p000 p=0.00 00 (p=0.001 p0000 p=0.000 000 (p=0.0001 2 p0 p<0. p=0. (p=0.0 (p=0.00 (p=0.000 p<0 p=0 (p=0. p< p= (p=0 (p= (p