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Integration of LBDD and SBDD Studies on Drug Design: A Fatty Acid Amide Hydrolase (FAAH) Case Study Design FAAH (FAAH
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Santana, Pedro A. L.
; Ruas, Marina S. M.
; Veríssimo, Gabriel C.
; Terra, Ana C. G.
; Oliveira, Renata B. de
; Maltarollo, Vinícius G.
.
Journal of the Brazilian Chemical Society
- Métricas do periódico
The inhibition of the fatty acid amide hydrolase (FAAH), an endocannabinoid system component, emerged as a potentially new therapeutic target for a range of clinical disorders such as acute and chronic pain. Some α-ketoheterocycle derivatives demonstrated interesting analgesic and anti-inflammatory activities in vitro. Ligand-Based Drug Design techniques such as knowledge graph convolutional networks (kGCN) and hologram quantitative structure-activity relationship (HQSAR) using α-ketoheterocycle derivatives from five different datasets were generated to discover the relation between the chemical structures and the inhibition activity. Meanwhile, structure-based drug design simulations as interaction fields (MIF), molecular docking, and ligand sites studies (LSI) from FAAH were performed using Autogrid software and FTmap/FTsite servers. The results of both studies were merged to propose predictive models. The resulting kGCN model demonstrated adequate accuracy area under the curve by receiver operating characteristic (AUC-ROC 0.7922). From contribution maps of the Ligand-Based Drug Design (LBDD) models and the generated probes using MIF and LSI, it was observed that the oxazole ring, the ketone group, and the apolar chain present in the structures of the inhibitors are important, besides the evidence of the Cys269 and Val270 residues importance for the potential interaction, confirmed by carried docking studies. These fragments and structural information can be used to carry out new FAAH potential inhibitors studies and report kGCN as an accurate classification technique. FAAH, , (FAAH) component pain αketoheterocycle α ketoheterocycle antiinflammatory anti inflammatory vitro LigandBased Ligand Based (kGCN structureactivity structure activity HQSAR (HQSAR Meanwhile structurebased based MIF, (MIF) LSI (LSI FTmapFTsite FTmap FTsite servers AUCROC AUC ROC 0.7922. 07922 0.7922 . 0 7922 0.7922) LBDD (LBDD ring group important Cys Cys26 Val Val27 technique (FAAH (MIF 0792 0.792 792 Cys2 Val2 079 0.79 79 07 0.7 7 0.
2.
Additively Manufactured Collector-Detector Device for Explosive Analysis Using Recycled PLA Filament Loaded with Carbon Black and Graphite CollectorDetector Collector Detector
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Souza, Maria M. C.
; Siqueira, Gilvana P.
; Rocha, Raquel G.
; Crapnell, Robert D.
; Santana, Mário H. P.
; Richter, Eduardo M.
; Banks, Craig E.
; Muñoz, Rodrigo A. A.
.
Journal of the Brazilian Chemical Society
- Métricas do periódico
We report the electrochemical potential of conductive filaments produced with recycled polylactic acid (rPLA) alongside a mixture of graphite (Gpt) and/or carbon black (CB) and castor oil to create an electrode ready-to-use. Additively-manufactured electrodes (AMEs) were compared with commercial-conductive filament composed of PLA and CB. The Gpt-CB-rPLA and CB-rPLA sensors showed lower charge-transfer resistance (Rct) and a greater heterogenous rate constant, k0, (Rct= 1040 ± 50 Ω and 1810 ± 30 Ω and k0= 6.91 (± 0.58) × 103 and 5.31 (± 0.40) × 103 cm s-1, respectively) compared to the sensor from the commercial filament (Rct = 9620 ± 280 Ω and k0 = 3.62 (± 0.38) ×10-3 cm s-1). The electrochemical response of [Fe(CN6)]3-/4- displayed a peak-to-peak separation of 180 ± 8 mV (Gpt-CB-rPLA) and 240 ± 6 mV (CB-rPLA) compared to commercial AME (740 ±10 mV), without any surface treatment. The filaments were used to create a 2,4,6-trinitrotoluene (TNT) detection platform with linear range of 5-20 and 5-100 µmol L-1 for Gpt-CB-rPLA and CB-rPLA, respectively. The collector-detector device was applied for the analysis of a blast simulation. Finally, it is worth noting that the results obtained are without electrode treatment/activation, indicating that the rough surface of the additively manufactured electrode is an additional feature of the device for collecting explosive residues, simply by rubbing the additively manufactured platform on different surfaces in crime scenarios. rPLA (rPLA Gpt (Gpt andor or CB (CB readytouse. readytouse ready use. use ready-to-use Additivelymanufactured Additively AMEs (AMEs commercialconductive GptCBrPLA CBrPLA chargetransfer charge transfer Rct constant k Rct= 104 5 181 3 691 91 6.9 ( 0.58 058 0 58 10 531 31 5.3 0.40 040 40 s1, s1 s 1, 1 s-1 respectively 962 28 362 62 3.6 0.38 038 38 ×103 ×10 ×10- s1. . s-1) FeCN63/4 FeCN634 FeCN Fe CN6 /4 4 CN [Fe(CN6)]3-/4 peaktopeak peak 18 (Gpt-CB-rPLA 24 (CB-rPLA 740 (74 ±1 mV, , mV) treatment 2,4,6trinitrotoluene 246trinitrotoluene trinitrotoluene 2,4,6 2 TNT (TNT 520 20 5-2 5100 100 5-10 L1 L L- CBrPLA, rPLA, collectordetector collector detector simulation Finally treatmentactivation activation treatment/activation residues scenarios 69 9 6. 0.5 05 53 5. 0.4 04 s- 96 36 3. 0.3 03 ×1 FeCN63 FeCN63/ / [Fe(CN6)]3-/ 74 (7 6trinitrotoluene 246 2,4, 52 5- 510 5-1 0. FeCN6 [Fe(CN6)]3- 7 2,4 51 [Fe(CN6)]3 2, [Fe(CN6)] [Fe(CN6) [Fe(CN6 [Fe(CN
3.
H2S removal at downhole conditions using iron oxide nanoparticles
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Mundo nano. Revista interdisciplinaria en nanociencias y nanotecnología
- Métricas do periódico
Resumen: El objetivo del presente trabajo es el estudio de la remoción de H2S de crudos pesados empleando nanopartículas de óxido de hierro en un ambiente controlado que simula las condiciones de presión y temperatura de un yacimiento y el proceso de acuatermólisis durante la recuperación mejorada de petróleo con inyección de vapor. Dado que la difusión molecular del H2S tiene un rol importante en el proceso de remoción, su medición experimental fue también un objetivo principal. La investigación se desarrolló en tres etapas: 1) preparación de las nanopartículas; 2) ensayos de difusión, y, 3) ensayos de remoción de H2S. El protocolo de síntesis de las nanopartículas a partir de una microemulsión y una sal metálica precursora generó exitosamente nanopartículas de tamaños inferiores a 100 nm. El coeficiente de difusión del H2S en el crudo pesado, medido en una celda PVT de acero inoxidable, varió entre 8.3 × 10-9 y 8.9 × 10-9 m2s-1 en el rango de temperaturas de las pruebas. Finalmente, más del 65% del H2S fue removido al usar 500 ppm de nanopartículas.
Abstract: The objective of the present work is the study of H2S removal from heavy oil, using iron oxide nanoparticles in a controlled environment that simulates the pressure and temperature conditions of a reservoir and the aqua-thermolysis process during enhanced oil recovery with steam injection. Since molecular diffusion of H2S plays an important role during the removal process, its measurement through experimental tests was also a major goal. The research divides into three stages: 1) preparation of nanoparticles; 2) diffusion tests, and, 3) H2S removal tests. The procedure for nanoparticle preparation from a microemulsion and a metal precursor salt was successful in yielding nanoparticle sizes less than 100 nm. The diffusion coefficient of H2S in heavy oil, measured in a stainless steel PVT cell, varied between 8.3 × 10-9 and 8.9 × 10-9 m2s-1 over the range of test temperatures. Finally, over 65% of the H2S was removed when 500 ppm of nanoparticles were used.
4.
Physiological and starch quality of potato tubers discarded from the potato chip industry in Mexico
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Montoya-Anaya, Diana G.
; Aguirre-Mancilla, César L.
; Rodríguez-Núñez, Jesús R.
; Madera-Santana, Tomás J.
; Covarrubias-Prieto, Jorge
; Gonzáles-García, Gerardo
; Gutiérrez-Benicio, Glenda M.
.
Chilean journal of agricultural research
- Métricas do periódico
ABSTRACT Agroindustrial potato (Solanum tuberosum L.) waste mainly includes peel and pulp; however, potatoes of low industrial quality are also discarded by industry. A viable alternative to give added value to these byproducts is the use of these as seeds, as well as the use of starch. The objective of this work was to evaluate the physiological quality (germination and vigor), starch content and relationship between these variables in discarded tubers from the industry through chemical-structural analyses to better understand their influence on starch composition. The physiological quality of the potatoes was evaluated through germination and vigor tests; likewise, starch was extracted, and the yield was determined. Physicochemical characterization of the starches was carried out through proximal and structural analysis using nuclear magnetic resonance (NMR). The results showed that the tubers had 100.00% germination and 98.66% vigor and were suitable for use as seeds. The starch yield did not significantly differ. However, characterization of the starch revealed significant differences (p ≤ 0.05) in moisture, ash, carbohydrate and amylose contents, which were influenced by the storage temperature (4 °C) of the tubers subjected to a vigor test, which interferes with enzymatic activity. During germination, the NMR results showed type B crystallinity, which is typical of tubers, indicating that the viability tests did not promote changes in the type of crystallinity. The results obtained can help us understand the effect of germination on the composition of starch and, accordingly, choose the most appropriate applications for this starch, whether food or nonfood.
5.
Kombucha and its dehydrated and freeze-dried derivatives: physicochemicaland microbiological characterization and in vivo toxicity evaluation
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Santana, Débora C.
; Danieleto, Fabiany B.
; Valdo, Kellen O.
; Costa, Clarisse M. Arpini
; Kalil, Ieda C.
; Andrade, Tadeu U.
; Bertolazi, Amanda Azevedo
; Passos, Heloisi G. S.
; Vasconcelos, Christiane M.
.
Resumen Kombucha es una biopelícula compuesta por un cultivo simbiótico de bacterias y levaduras, conocido como SCOBY (Symbiotic Culture of Bacteria and Yeast). El SCOBY sufre procesos de deterioro y pérdida de atributos nutricionales, bioactivos y sensoriales, lo que impacta negativamente en su vida útil. Estas pérdidas pueden evitarse mediante métodos de secado, que ayudan a prolongar la vida útil y conservar los compuestos de acción de sus constituyentes. El objetivo de este estudio fue someter y evaluar Kombucha SCOBY a las técnicas de deshidratación por calor y liofilización. Los análisis incluyeron pH, sólidos solubles totales, acidez titulable total, turbidez, contenido de compuestos fenólicos totales, actividad antioxidante, evaluación microbiológica y pruebas de toxicidad in vivo. Todos los análisis fueron evaluados estadísticamente a un nivel de probabilidad del 5%, utilizando el software SAS. Tanto las bebidas de Kombucha como los productos secos exhibieron cantidades considerables de compuestos fenólicos y actividad antioxidante. Los recuentos de microorganismos oscilaron entre 104 y 108 CFU.ml-1 para bebidas y productos desarrollados. En las pruebas in vivo con Caenorhabditis elegans, no se observó un aumento en la mortalidad y los gusanos alimentados con los productos fermentados mostraron un mayor crecimiento. Por lo tanto, este estudio concluye que la producción de formas deshidratadas y liofilizadas de productos a base de Kombucha es un campo de investigación prometedor y ventajoso para la salud humana.
Abstract Kombucha is a biofilm composed of a symbiotic culture of bacteria and yeast, known as SCOBY (Symbiotic Culture of Bacteria and Yeast). The SCOBY undergoes deterioration processes and loss of nutritional, bioactive and sensory attributes, which negatively impacts its shelf life. These losses can be avoided by drying methods, which help to extend shelf life and preserve the action compounds of its constituents. The objective of this study was to subject and evaluate Kombucha SCOBY to drying techniques by heat dehydration and freeze-drying. Analyses included pH, total soluble solids, total titratable acidity, turbidity, total phenolic compound content, antioxidant activity, microbiological assessment, and toxicity in vivo testing. All analyses were statistically evaluated at a 5% probability level, using SAS software. Both Kombucha beverages and dry products exhibited considerable amounts of phenolic compounds and antioxidant activity. Microorganism counts ranged from 104 to 108 CFU.ml-1 for developed beverages and products. In In vivo tests with Caenorhabditis elegans, no increase in mortality was observed, and worms fed with the fermented products showed enhanced growth. Thus, this study concludes that producing dehydrated and freeze-dried forms of Kombucha-based products is a promising and advantageous field of researchfor human health.
6.
Genetic diversity based on multivariate techniques in elephant grass genotypes for bioenergy
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Vidal, A. K. F.
; Daher, R. F.
; Freitas, R. S.
; Santana, J. G. S.
; Stida, W. F.
; Ambrósio, M.
; Silva, V. B.
; Souza, A. G.
; Nascimento, M. R.
; Santos, R. M.
; Leite, C. L.
; Farias, J. E. C.
.
Resumo A utilização de biomassa para a produção de energia representa uma estratégia promissora, justificando a busca por novas fontes de biomassa. O capim-elefante tem ganhado destaque devido ao seu elevado rendimento de matéria seca e rápido crescimento. Este estudo teve como objetivo quantificar a divergência genética entre novas famílias de meio-irmãos de capim-elefante, com a finalidade de identificar genótipos com maior divergência genética e potencial produtivo para hibridização. Utilizou-se uma metodologia de agrupamento hierárquico baseada em componentes principais. As famílias de meio-irmãos foram obtidas a partir de genótipos do Banco Ativo de Germoplasma de Capim-Elefante. O experimento foi conduzido em delineamento de blocos casualizados, com nove famílias de meio-irmãos, três repetições e oito plantas por parcela, totalizando a avaliação de 216 genótipos de capim-elefante. Foram realizadas análises de componentes principais (PC), biplot e agrupamento hierárquico para estimar a diversidade, utilizando o software R. Os dois primeiros PCs da análise biplot explicaram 64% da variação total. A produtividade de matéria seca foi a característica mais relevante para a discriminação genotípica (0,89), seguida pela altura da planta (0,67) e pelo diâmetro do caule (0,61) no PC1. Nesta análise, foram consideradas as distâncias entre os acessos, sem considerar parentesco, o que indica uma variabilidade significativa dentro das famílias avaliadas, uma vez que os genótipos pertencentes à mesma família não foram agrupados, mas sim distribuídos em grupos distintos. promissora capimelefante capim elefante crescimento meioirmãos meio irmãos capimelefante, elefante, hibridização Utilizouse Utilizou se CapimElefante. CapimElefante Capim Elefante. Elefante Capim-Elefante casualizados meioirmãos, irmãos, parcela 21 capimelefante. elefante. PC, PC , (PC) diversidade R 64 total 0,89, 089 0,89 0 89 (0,89) 0,67 067 67 (0,67 0,61 061 61 (0,61 PC1 acessos parentesco avaliadas agrupados distintos 2 (PC 6 08 0,8 8 (0,89 0,6 06 (0,6 0, (0,8 (0, (0 (
Abstract The use of biomass for energy production constitutes a promising strategy that warrants the search for new sources of biomass. Elephant grass has been gaining notoriety due to its high dry matter yield and rapid growth. The present study was carried out to quantify the genetic divergence of nine elephant grass half-sib families in order to identify genotypes with greater genetic divergence and productive potential for hybridization, using the hierarchical clustering methodology based on principal components. Half-sib families were generated using genotypes from the Active Germplasm Bank of Elephant Grass. The experiment was laid out in a randomized-block design with nine half-sib families, three replicates, and eight plants per plot. A total of 216 genotypes of elephant grass were evaluated. Principal component (PC), biplot, and hierarchical clustering analyses for diversity estimation were conducted using R software. The first two PCs of biplot analysis accounted for 64% of the cumulative variation. Dry matter yield was the most important trait for genotype discrimination (0.89), followed by plant height (0.67) and stem diameter (0.61) in PC1. In this analysis, the distances between accessions were considered and there were no family links, which indicates the existence of wide variability within the evaluated families, since genotypes belonging to the same family were not grouped together, but rather distributed into different groups. Crosses between genotypes of group three and genotypes of groups one and two are recommended for the development of high-yielding genotypes when aiming at energy production. growth halfsib half sib hybridization components Halfsib Half Grass randomizedblock randomized block replicates plot 21 PC, PC , (PC) software 64 variation 0.89, 089 0.89 0 89 (0.89) 0.67 067 67 (0.67 0.61 061 61 (0.61 PC1 links together highyielding yielding 2 (PC 6 08 0.8 8 (0.89 0.6 06 (0.6 0. (0.8 (0. (0 (
7.
Radiation Balance over Low-Turbidity Water Artificially Cleaned for Irrigation of Tobacco Grown Under Shading. I. Analysis of the Shortwave and Longwave Components LowTurbidity Low Turbidity Shading I
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Borges, Tatyana Keyty de Souza
; Oliveira, Aureo Silva de
; Allen, Richard G.
; Kilic, Ayse
; Couto, João Paulo Chaves
; Santana, Carlos Eduardo
.
Resumo No leste subúmido da Bahia, Brasil, água de superfície armazenada em reservatórios revestidos é usada para irrigar por gotejamento plantas de fumo tipo Sumatra cultivadas sob sombreamento parcial. Este trabalho, no âmbito de um estudo maior sobre evaporação, teve como objetivo monitorar, durante duas estações de cultivo (2015 e 2016), a turbidez da água, o balanço de radiação (componentes de onda curta - OC e onda longa - OL) e a temperatura superficial da água (Tw), com sensores instalados numa plataforma flutuante. A turbidez da água era muito baixa (cerca de 2,6 NTU em média) devido à filtragem e tratamento osmose reversa antes do armazenamento. Com base nos dados de ambas as estações, a radiação líquida de OC Snet correspondeu a cerca de 96% da radiação de OC incidente Sg. O saldo diário de OL Lnet foi consistentemente negativo (média de -55,1 W/m2). A radiação líquida total Rn correspondeu a 77% de Snet. Quatro dias foram selecionados (dois de cada ano) para avaliar os efeitos da nebulosidade nos componentes de OC e OL. Enquanto Snet diminuiu com a nebulosidade devido ao bloqueio da radiação solar, Lnet também diminuiu, mas devido ao aumento da radiação OL incidente Latm com a nebulosidade. Rn também diminuiu com a cobertura de nuvens. Com base nos dados de 2015, em média, a Tw diária (26,1 °C) foi maior que a temperatura do ar Ta (21,8 °C) devido à absorção da radiação solar pela superfície, Ta mostrando uma amplitude diária muito maior. Bahia Brasil parcial trabalho evaporação monitorar 2015 (201 2016, 2016 , 2016) Tw, (Tw) flutuante 26 2 6 2, média armazenamento 96 Sg 55,1 551 55 1 -55, W/m2. Wm2 Wm W/m2 . W m2 m W/m2) 77 dois ano nuvens 26,1 261 (26, °C C 21,8 218 21 8 (21, 201 (20 (Tw 9 55, 5 -55 W/m 7 26, (26 21, (21 20 (2 -5 (
Abstract In eastern sub-humid Bahia, Brazil, surface water stored in lined ponds is used to drip irrigate Sumatra tobacco plants grown under partial shading. This work, as part of a large evaporation study, aimed at monitoring, over two irrigation seasons (2015 and 2016), the water turbidity, the radiation balance (shortwave - SW and longwave - LW components), and the skin water temperature (Tw) with sensors deployed in a raft. The water turbidity was very low (around 2.6 NTU on average) due to filtration and reverse osmosis treatment of water prior to storage. Considering data from both seasons, the net SW radiation Snet was about 96% of incoming SW flux Sg. The daily net LW flux Lnet was consistently negative (-55.1 W/m2 average). The net all-wave radiation Rn corresponded to 77% of Snet. Four days were selected from both seasons to evaluate the effects of cloudiness on the SW and LW components. While Snet decreased with cloudiness due to solar radiation blockage, Lnet also decreased but due to increasing of downward LW radiation Latm with cloudiness. Rn also decreased with cloud cover. Based on the 2015 data, on average, daily Tw (26.1 °C) was higher than air temperature Ta (21.8 °C) due to near-surface absorption of solar radiation with Ta showing a much higher daily amplitude. subhumid sub humid Bahia Brazil shading work study monitoring (201 2016, 2016 , 2016) shortwave components, components components) (Tw raft around 26 2 6 2. average storage 96 Sg 55.1 551 55 1 (-55. Wm2 Wm W m2 m W/m average. . allwave all wave 77 blockage cover 201 26.1 261 (26. °C C 21.8 218 21 8 (21. nearsurface near amplitude (20 9 55. 5 (-55 7 20 26. (26 21. (21 (2 (-5 ( (-
8.
Radiation Balance over Low-Turbidity Water Artificially Cleaned for Irrigation of Tobacco Grown Under Shading. II. Water Albedo Analysis and Net Radiation Modelling LowTurbidity Low Turbidity Shading II
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Borges, Tatyana Keyty de Souza
; Oliveira, Aureo Silva de
; Allen, Richard G.
; Kilic, Ayse
; Couto, João Paulo Chaves
; Santana, Carlos Eduardo
.
Resumo O albedo de uma superfície de água e a energia disponível para evaporação estão fortemente correlacionados e estudar tais processos é de suma importância para a segurança hídrica nível local e regional. Neste artigo, o albedo da água (αw) e a radiação líquida (Rn) foram analisados após serem medidos acima da superfície de água artificialmente limpa e de baixa turbidez usada para irrigação de cultivo de fumo. Observou-se que αw diminuiu com o aumento da elevação do sol (θ), especialmente para céu limpo e quase limpo. Os resultados mostraram que αw pode ser razoavelmente previsto com um modelo potencial em termos de θ ou Sg (radiação solar incidente) em diferentes condições de cobertura de nuvens. A partir deste estudo, um albedo médio diário de 0,05 é recomendado. Três abordagens foram consideradas para estimativa diária de Rn. Na primeira, um modelo de regressão linear ajustou-se fortemente aos dados de Rn em função de Sg, apenas. A segunda opção com base na definição de Rn foi [0,95Sg - Lnet(56)], onde Lnet(56) é a radiação líquida de onda longa do modelo FAO56 para estimativa da evapotranspiração de referência, e a terceira abordagem foi [0,95Sg - Lnet(MLR)], onde MLR significa regressão linear múltipla. As desvantagens das abordagens (1) e (3), baseadas em regressões, é que elas são restritas ao tipo de água armazenada na fazenda e às condições climáticas da região. O desempenho da abordagem (2), onde Lnet(56) é um modelo amplamente utilizado, foi comparável às outras e pode ser potencialmente melhorada com calibração local. Todas as três abordagens para estimar o Rn diário propostas neste estudo podem ser estendidas para águas claras que não passaram por nenhum processo de filtragem. regional artigo (αw (Rn fumo Observouse Observou se θ, , (θ) incidente nuvens 005 0 05 0,0 recomendado primeira ajustouse ajustou apenas 0,95Sg 095Sg 95Sg Lnet56, Lnet56 Lnet 56 Lnet(56)] Lnet(56 FAO FAO5 referência LnetMLR, LnetMLR Lnet(MLR)] múltipla 1 (1 3, 3 (3) regressões região 2, 2 (2) utilizado filtragem (θ 00 0, Lnet5 5 Lnet(5 Lnet(MLR) ( (3 (2 Lnet( Lnet(MLR
Abstract The albedo of a water surface and the energy available for evaporation are strongly correlated and studying such processes is of paramount importance for water security at farm and regional levels. In this paper, water albedo (αw) and net all-wave radiation (Rn) were analyzed after being measured above the surface of an artificially cleaned and low-turbidity water used for tobacco irrigation. It was observed that αw decreased as the sun elevation (θ) increased, especially for clear and near clear skies. The results showed that αw can be reasonably predicted with a power law model either in terms of θ or Sg (incoming solar radiation) across different cloud cover conditions. From this study, a mean daily albedo of 0.05 is recommended. Three approaches were considered for estimation of daily Rn. In the first, a linear regression model strongly fitted Rn data in terms of Sg solely. The second option based on the definition of Rn was [0.95Sg - Lnet(56)], where Lnet(56) is net longwave (LW) radiation as used in the FAO56 model for reference evapotranspiration estimation, and the third was [0.95Sg - Lnet(MLR)], where MLR stands for multiple linear regression. The disadvantages of approaches (1) and (3), based on regressions, is that they are constrained to the type of water stored in the farm and the climatic conditions of the region. The performance of approach (2), where Lnet(56) is a widely used model, was comparable to the others can potentially be improved with a site-specific calibration. All three approaches for estimating daily Rn proposed in this study can possibily be extended to clear water that did not go through any filtration process. levels paper (αw allwave all wave (Rn lowturbidity low turbidity irrigation (θ increased skies incoming 005 0 05 0.0 recommended first solely 0.95Sg 095Sg 95Sg Lnet56, Lnet56 Lnet 56 , Lnet(56)] Lnet(56 LW (LW FAO FAO5 LnetMLR, LnetMLR Lnet(MLR)] 1 (1 3, 3 (3) regressions region 2, 2 (2) sitespecific site specific calibration process 00 0. Lnet5 5 Lnet(5 Lnet(MLR) ( (3 (2 Lnet( Lnet(MLR
9.
Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil Brasil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
; Slipinski, Adam
; Linzmeier, Adelita M.
; Calor, Adolfo R.
; Garda, Adrian A.
; Kury, Adriano B.
; Fernandes, Agatha C.S.
; Agudo-Padrón, Aisur I.
; Akama, Alberto
; Silva Neto, Alberto M. da
; Burbano, Alejandro L.
; Menezes, Aleksandra
; Pereira-Colavite, Alessandre
; Anichtchenko, Alexander
; Lees, Alexander C.
; Bezerra, Alexandra M.R.
; Domahovski, Alexandre C.
; Pimenta, Alexandre D.
; Aleixo, Alexandre L.P.
; Marceniuk, Alexandre P.
; Paula, Alexandre S. de
; Somavilla, Alexandre
; Specht, Alexandre
; Camargo, Alexssandro
; Newton, Alfred F.
; Silva, Aline A.S. da
; Santos, Aline B. dos
; Tassi, Aline D.
; Aragão, Allan C.
; Santos, Allan P.M.
; Migotto, Alvaro E.
; Mendes, Amanda C.
; Cunha, Amanda
; Chagas Júnior, Amazonas
; Sousa, Ana A.T. de
; Pavan, Ana C.
; Almeida, Ana C.S.
; Peronti, Ana L.B.G.
; Henriques-Oliveira, Ana L.
; Prudente, Ana L.
; Tourinho, Ana L.
; Pes, Ana M.O.
; Carmignotto, Ana P.
; Wengrat, Ana P.G. da Silva
; Dornellas, Ana P.S.
; Molin, Anamaria Dal
; Puker, Anderson
; Morandini, André C.
; Ferreira, André da S.
; Martins, André L.
; Esteves, André M.
; Fernandes, André S.
; Roza, André S.
; Köhler, Andreas
; Paladini, Andressa
; Andrade, Andrey J. de
; Pinto, Ângelo P.
; Salles, Anna C. de A.
; Gondim, Anne I.
; Amaral, Antonia C.Z.
; Rondón, Antonio A.A.
; Brescovit, Antonio
; Lofego, Antônio C.
; Marques, Antonio C.
; Macedo, Antonio
; Andriolo, Artur
; Henriques, Augusto L.
; Ferreira Júnior, Augusto L.
; Lima, Aurino F. de
; Barros, Ávyla R. de A.
; Brito, Ayrton do R.
; Romera, Bárbara L.V.
; Vasconcelos, Beatriz M.C. de
; Frable, Benjamin W.
; Santos, Bernardo F.
; Ferraz, Bernardo R.
; Rosa, Brunno B.
; Sampaio, Brunno H.L.
; Bellini, Bruno C.
; Clarkson, Bruno
; Oliveira, Bruno G. de
; Corrêa, Caio C.D.
; Martins, Caleb C.
; Castro-Guedes, Camila F. de
; Souto, Camilla
; Bicho, Carla de L.
; Cunha, Carlo M.
; Barboza, Carlos A. de M.
; Lucena, Carlos A.S. de
; Barreto, Carlos
; Santana, Carlos D.C.M. de
; Agne, Carlos E.Q.
; Mielke, Carlos G.C.
; Caetano, Carlos H.S.
; Flechtmann, Carlos H.W.
; Lamas, Carlos J.E.
; Rocha, Carlos
; Mascarenhas, Carolina S.
; Margaría, Cecilia B.
; Waichert, Cecilia
; Digiani, Celina
; Haddad, Célio F.B.
; Azevedo, Celso O.
; Benetti, Cesar J.
; Santos, Charles M.D. dos
; Bartlett, Charles R.
; Bonvicino, Cibele
; Ribeiro-Costa, Cibele S.
; Santos, Cinthya S.G.
; Justino, Cíntia E.L.
; Canedo, Clarissa
; Bonecker, Claudia C.
; Santos, Cláudia P.
; Carvalho, Claudio J.B. de
; Gonçalves, Clayton C.
; Galvão, Cleber
; Costa, Cleide
; Oliveira, Cléo D.C. de
; Schwertner, Cristiano F.
; Andrade, Cristiano L.
; Pereira, Cristiano M.
; Sampaio, Cristiano
; Dias, Cristina de O.
; Lucena, Daercio A. de A.
; Manfio, Daiara
; Amorim, Dalton de S.
; Queiroz, Dalva L. de
; Queiroz, Dalva L. de
; Colpani, Daniara
; Abbate, Daniel
; Aquino, Daniel A.
; Burckhardt, Daniel
; Cavallari, Daniel C.
; Prado, Daniel de C. Schelesky
; Praciano, Daniel L.
; Basílio, Daniel S.
; Bená, Daniela de C.
; Toledo, Daniela G.P. de
; Takiya, Daniela M.
; Fernandes, Daniell R.R.
; Ament, Danilo C.
; Cordeiro, Danilo P.
; Silva, Darliane E.
; Pollock, Darren A.
; Muniz, David B.
; Gibson, David I.
; Nogueira, David S.
; Marques, Dayse W.A.
; Lucatelli, Débora
; Garcia, Deivys M.A.
; Baêta, Délio
; Ferreira, Denise N.M.
; Rueda-Ramírez, Diana
; Fachin, Diego A.
; Souza, Diego de S.
; Rodrigues, Diego F.
; Pádua, Diego G. de
; Barbosa, Diego N.
; Dolibaina, Diego R.
; Amaral, Diogo C.
; Chandler, Donald S.
; Maccagnan, Douglas H.B.
; Caron, Edilson
; Carvalho, Edrielly
; Adriano, Edson A.
; Abreu Júnior, Edson F. de
; Pereira, Edson H.L.
; Viegas, Eduarda F.G.
; Carneiro, Eduardo
; Colley, Eduardo
; Eizirik, Eduardo
; Santos, Eduardo F. dos
; Shimbori, Eduardo M.
; Suárez-Morales, Eduardo
; Arruda, Eliane P. de
; Chiquito, Elisandra A.
; Lima, Élison F.B.
; Castro, Elizeu B. de
; Orlandin, Elton
; Nascimento, Elynton A. do
; Razzolini, Emanuel
; Gama, Emanuel R.R.
; Araujo, Enilma M. de
; Nishiyama, Eric Y.
; Spiessberger, Erich L.
; Santos, Érika C.L. dos
; Contreras, Eugenia F.
; Galati, Eunice A.B.
; Oliveira Junior, Evaldo C. de
; Gallardo, Fabiana
; Hernandes, Fabio A.
; Lansac-Tôha, Fábio A.
; Pitombo, Fabio B.
; Dario, Fabio Di
; Santos, Fábio L. dos
; Mauro, Fabio
; Nascimento, Fabio O. do
; Olmos, Fabio
; Amaral, Fabio R.
; Schunck, Fabio
; Godoi, Fábio S. P. de
; Machado, Fabrizio M.
; Barbo, Fausto E.
; Agrain, Federico A.
; Ribeiro, Felipe B.
; Moreira, Felipe F.F.
; Barbosa, Felipe F.
; Silva, Fenanda S.
; Cavalcanti, Fernanda F.
; Straube, Fernando C.
; Carbayo, Fernando
; Carvalho Filho, Fernando
; Zanella, Fernando C.V.
; Jacinavicius, Fernando de C.
; Farache, Fernando H.A.
; Leivas, Fernando
; Dias, Fernando M.S.
; Mantellato, Fernando
; Vaz-de-Mello, Fernando Z.
; Gudin, Filipe M.
; Albuquerque, Flávio
; Molina, Flavio B.
; Passos, Flávio D.
; Shockley, Floyd W.
; Pinheiro, Francielly F.
; Mello, Francisco de A.G. de
; Nascimento, Francisco E. de L.
; Franco, Francisco L.
; Oliveira, Francisco L. de
; Melo, Francisco T. de V.
; Quijano, Freddy R.B.
; Salles, Frederico F.
; Biffi, Gabriel
; Queiroz, Gabriel C.
; Bizarro, Gabriel L.
; Hrycyna, Gabriela
; Leviski, Gabriela
; Powell, Gareth S.
; Santos, Geane B. dos
; Morse, Geoffrey E.
; Brown, George
; Mattox, George M.T.
; Zimbrão, Geraldo
; Carvalho, Gervásio S.
; Miranda, Gil F.G.
; Moraes, Gilberto J. de
; Lourido, Gilcélia M.
; Neves, Gilmar P.
; Moreira, Gilson R.P.
; Montingelli, Giovanna G.
; Maurício, Giovanni N.
; Marconato, Gláucia
; Lopez, Guilherme E.L.
; Silva, Guilherme L. da
; Muricy, Guilherme
; Brito, Guilherme R.R.
; Garbino, Guilherme S.T.
; Flores, Gustavo E.
; Graciolli, Gustavo
; Libardi, Gustavo S.
; Proctor, Heather C.
; Gil-Santana, Helcio R.
; Varella, Henrique R.
; Escalona, Hermes E.
; Schmitz, Hermes J.
; Rodrigues, Higor D.D.
; Galvão Filho, Hilton de C.
; Quintino, Hingrid Y.S.
; Pinto, Hudson A.
; Rainho, Hugo L.
; Miyahira, Igor C.
; Gonçalves, Igor de S.
; Martins, Inês X.
; Cardoso, Irene A.
; Oliveira, Ismael B. de
; Franz, Ismael
; Fernandes, Itanna O.
; Golfetti, Ivan F.
; S. Campos-Filho, Ivanklin
; Oliveira, Ivo de S.
; Delabie, Jacques H.C.
; Oliveira, Jader de
; Prando, Jadila S.
; Patton, James L.
; Bitencourt, Jamille de A.
; Silva, Janaina M.
; Santos, Jandir C.
; Arruda, Janine O.
; Valderrama, Jefferson S.
; Dalapicolla, Jeronymo
; Oliveira, Jéssica P.
; Hájek, Jiri
; Morselli, João P.
; Narita, João P.
; Martin, João P.I.
; Grazia, Jocélia
; McHugh, Joe
; Cherem, Jorge J.
; Farias Júnior, José A.S.
; Fernandes, Jose A.M.
; Pacheco, José F.
; Birindelli, José L.O.
; Rezende, José M.
; Avendaño, Jose M.
; Duarte, José M. Barbanti
; Ribeiro, José R. Inácio
; Mermudes, José R.M.
; Pujol-Luz, José R.
; Santos, Josenilson R. dos
; Câmara, Josenir T.
; Teixeira, Joyce A.
; Prado, Joyce R. do
; Botero, Juan P.
; Almeida, Julia C.
; Kohler, Julia
; Gonçalves, Julia P.
; Beneti, Julia S.
; Donahue, Julian P.
; Alvim, Juliana
; Almeida, Juliana C.
; Segadilha, Juliana L.
; Wingert, Juliana M.
; Barbosa, Julianna F.
; Ferrer, Juliano
; Santos, Juliano F. dos
; Kuabara, Kamila M.D.
; Nascimento, Karine B.
; Schoeninger, Karine
; Campião, Karla M.
; Soares, Karla
; Zilch, Kássia
; Barão, Kim R.
; Teixeira, Larissa
; Sousa, Laura D. do N.M. de
; Dumas, Leandro L.
; Vieira, Leandro M.
; Azevedo, Leonardo H.G.
; Carvalho, Leonardo S.
; Souza, Leonardo S. de
; Rocha, Leonardo S.G.
; Bernardi, Leopoldo F.O.
; Vieira, Letícia M.
; Johann, Liana
; Salvatierra, Lidianne
; Oliveira, Livia de M.
; Loureiro, Lourdes M.A. El-moor
; Barreto, Luana B.
; Barros, Luana M.
; Lecci, Lucas
; Camargos, Lucas M. de
; Lima, Lucas R.C.
; Almeida, Lucia M.
; Martins, Luciana R.
; Marinoni, Luciane
; Moura, Luciano de A.
; Lima, Luciano
; Naka, Luciano N.
; Miranda, Lucília S.
; Salik, Lucy M.
; Bezerra, Luis E.A.
; Silveira, Luis F.
; Campos, Luiz A.
; Castro, Luiz A.S. de
; Pinho, Luiz C.
; Silveira, Luiz F.L.
; Iniesta, Luiz F.M.
; Tencatt, Luiz F.C.
; Simone, Luiz R.L.
; Malabarba, Luiz R.
; Cruz, Luiza S. da
; Sekerka, Lukas
; Barros, Lurdiana D.
; Santos, Luziany Q.
; Skoracki, Maciej
; Correia, Maira A.
; Uchoa, Manoel A.
; Andrade, Manuella F.G.
; Hermes, Marcel G.
; Miranda, Marcel S.
; Araújo, Marcel S. de
; Monné, Marcela L.
; Labruna, Marcelo B.
; Santis, Marcelo D. de
; Duarte, Marcelo
; Knoff, Marcelo
; Nogueira, Marcelo
; Britto, Marcelo R. de
; Melo, Marcelo R.S. de
; Carvalho, Marcelo R. de
; Tavares, Marcelo T.
; Kitahara, Marcelo V.
; Justo, Marcia C.N.
; Botelho, Marcia J.C.
; Couri, Márcia S.
; Borges-Martins, Márcio
; Felix, Márcio
; Oliveira, Marcio L. de
; Bologna, Marco A.
; Gottschalk, Marco S.
; Tavares, Marcos D.S.
; Lhano, Marcos G.
; Bevilaqua, Marcus
; Santos, Marcus T.T.
; Domingues, Marcus V.
; Sallum, Maria A.M.
; Digiani, María C.
; Santarém, Maria C.A.
; Nascimento, Maria C. do
; Becerril, María de los A.M.
; Santos, Maria E.A. dos
; Passos, Maria I. da S. dos
; Felippe-Bauer, Maria L.
; Cherman, Mariana A.
; Terossi, Mariana
; Bartz, Marie L.C.
; Barbosa, Marina F. de C.
; Loeb, Marina V.
; Cohn-Haft, Mario
; Cupello, Mario
; Martins, Marlúcia B.
; Christofersen, Martin L.
; Bento, Matheus
; Rocha, Matheus dos S.
; Martins, Maurício L.
; Segura, Melissa O.
; Cardenas, Melissa Q.
; Duarte, Mércia E.
; Ivie, Michael A.
; Mincarone, Michael M.
; Borges, Michela
; Monné, Miguel A.
; Casagrande, Mirna M.
; Fernandez, Monica A.
; Piovesan, Mônica
; Menezes, Naércio A.
; Benaim, Natalia P.
; Reategui, Natália S.
; Pedro, Natan C.
; Pecly, Nathalia H.
; Ferreira Júnior, Nelson
; Silva Júnior, Nelson J. da
; Perioto, Nelson W.
; Hamada, Neusa
; Degallier, Nicolas
; Chao, Ning L.
; Ferla, Noeli J.
; Mielke, Olaf H.H.
; Evangelista, Olivia
; Shibatta, Oscar A.
; Oliveira, Otto M.P.
; Albornoz, Pablo C.L.
; Dellapé, Pablo M.
; Gonçalves, Pablo R.
; Shimabukuro, Paloma H.F.
; Grossi, Paschoal
; Rodrigues, Patrícia E. da S.
; Lima, Patricia O.V.
; Velazco, Paul
; Santos, Paula B. dos
; Araújo, Paula B.
; Silva, Paula K.R.
; Riccardi, Paula R.
; Garcia, Paulo C. de A.
; Passos, Paulo G.H.
; Corgosinho, Paulo H.C.
; Lucinda, Paulo
; Costa, Paulo M.S.
; Alves, Paulo P.
; Roth, Paulo R. de O.
; Coelho, Paulo R.S.
; Duarte, Paulo R.M.
; Carvalho, Pedro F. de
; Gnaspini, Pedro
; Souza-Dias, Pedro G.B.
; Linardi, Pedro M.
; Bartholomay, Pedro R.
; Demite, Peterson R.
; Bulirsch, Petr
; Boll, Piter K.
; Pereira, Rachel M.M.
; Silva, Rafael A.P.F.
; Moura, Rafael B. de
; Boldrini, Rafael
; Silva, Rafaela A. da
; Falaschi, Rafaela L.
; Cordeiro, Ralf T.S.
; Mello, Ramon J.C.L.
; Singer, Randal A.
; Querino, Ranyse B.
; Heleodoro, Raphael A.
; Castilho, Raphael de C.
; Constantino, Reginaldo
; Guedes, Reinaldo C.
; Carrenho, Renan
; Gomes, Renata S.
; Gregorin, Renato
; Machado, Renato J.P.
; Bérnils, Renato S.
; Capellari, Renato S.
; Silva, Ricardo B.
; Kawada, Ricardo
; Dias, Ricardo M.
; Siewert, Ricardo
; Brugnera, Ricaro
; Leschen, Richard A.B.
; Constantin, Robert
; Robbins, Robert
; Pinto, Roberta R.
; Reis, Roberto E. dos
; Ramos, Robson T. da C.
; Cavichioli, Rodney R.
; Barros, Rodolfo C. de
; Caires, Rodrigo A.
; Salvador, Rodrigo B.
; Marques, Rodrigo C.
; Araújo, Rodrigo C.
; Araujo, Rodrigo de O.
; Dios, Rodrigo de V.P.
; Johnsson, Rodrigo
; Feitosa, Rodrigo M.
; Hutchings, Roger W.
; Lara, Rogéria I.R.
; Rossi, Rogério V.
; Gerstmeier, Roland
; Ochoa, Ronald
; Hutchings, Rosa S.G.
; Ale-Rocha, Rosaly
; Rocha, Rosana M. da
; Tidon, Rosana
; Brito, Rosangela
; Pellens, Roseli
; Santos, Sabrina R. dos
; Santos, Sandra D. dos
; Paiva, Sandra V.
; Santos, Sandro
; Oliveira, Sarah S. de
; Costa, Sávio C.
; Gardner, Scott L.
; Leal, Sebastián A. Muñoz
; Aloquio, Sergio
; Bonecker, Sergio L.C.
; Bueno, Sergio L. de S.
; Almeida, Sérgio M. de
; Stampar, Sérgio N.
; Andena, Sérgio R.
; Posso, Sergio R.
; Lima, Sheila P.
; Gadelha, Sian de S.
; Thiengo, Silvana C.
; Cohen, Simone C.
; Brandão, Simone N.
; Rosa, Simone P.
; Ribeiro, Síria L.B.
; Letana, Sócrates D.
; Santos, Sonia B. dos
; Andrade, Sonia C.S.
; Dávila, Stephane
; Vaz, Stéphanie
; Peck, Stewart B.
; Christo, Susete W.
; Cunha, Suzan B.Z.
; Gomes, Suzete R.
; Duarte, Tácio
; Madeira-Ott, Taís
; Marques, Taísa
; Roell, Talita
; Lima, Tarcilla C. de
; Sepulveda, Tatiana A.
; Maria, Tatiana F.
; Ruschel, Tatiana P.
; Rodrigues, Thaiana
; Marinho, Thais A.
; Almeida, Thaís M. de
; Miranda, Thaís P.
; Freitas, Thales R.O.
; Pereira, Thalles P.L.
; Zacca, Thamara
; Pacheco, Thaynara L.
; Martins, Thiago F.
; Alvarenga, Thiago M.
; Carvalho, Thiago R. de
; Polizei, Thiago T.S.
; McElrath, Thomas C.
; Henry, Thomas
; Pikart, Tiago G.
; Porto, Tiago J.
; Krolow, Tiago K.
; Carvalho, Tiago P.
; Lotufo, Tito M. da C.
; Caramaschi, Ulisses
; Pinheiro, Ulisses dos S.
; Pardiñas, Ulyses F.J.
; Maia, Valéria C.
; Tavares, Valeria
; Costa, Valmir A.
; Amaral, Vanessa S. do
; Silva, Vera C.
; Wolff, Vera R. dos S.
; Slobodian, Verônica
; Silva, Vinícius B. da
; Espíndola, Vinicius C.
; Costa-Silva, Vinicius da
; Bertaco, Vinicius de A.
; Padula, Vinícius
; Ferreira, Vinicius S.
; Silva, Vitor C.P. da
; Piacentini, Vítor de Q.
; Sandoval-Gómez, Vivian E.
; Trevine, Vivian
; Sousa, Viviane R.
; Sant’Anna, Vivianne B. de
; Mathis, Wayne N.
; Souza, Wesley de O.
; Colombo, Wesley D.
; Tomaszewska, Wioletta
; Wosiacki, Wolmar B.
; Ovando, Ximena M.C.
; Leite, Yuri L.R.
.
ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others. publications problem uptodate up date classifications context exception (CTFB http//fauna.jbrj.gov.br/, httpfaunajbrjgovbr http //fauna.jbrj.gov.br/ , jbrj gov br (http://fauna.jbrj.gov.br/) 2015 Brazil 80 specialists 1 2024 133691 133 691 133,69 125138 125 138 125,13 82.3%, 823 82 3 (82.3% 102000 102 000 102,00 7.69%, 769 7 69 (7.69% 11000 11 11,00 . 3,567 3567 567 (3,56 2,292 2292 2 292 (2,29 1,833 1833 833 (1,83 1,447 1447 447 (1,44 1000 1,00 831 (83 628 (62 606 (60 520 (52 50 users science health biology law anthropology education others http//fauna.jbrj.gov.br/ faunajbrjgovbr //fauna.jbrj.gov.br (http://fauna.jbrj.gov.br/ 201 8 202 13369 13 133,6 12513 12 125,1 82.3% (82.3 10200 10 00 102,0 7.69% 76 6 (7.69 1100 11,0 3,56 356 56 (3,5 2,29 229 29 (2,2 1,83 183 83 (1,8 1,44 144 44 (1,4 100 1,0 (8 62 (6 60 52 (5 5 http//fauna.jbrj.gov.br (http://fauna.jbrj.gov.br 20 1336 133, 1251 125, 82.3 (82. 1020 0 102, 7.69 (7.6 110 11, 3,5 35 (3, 2,2 22 (2, 1,8 18 (1, 1,4 14 4 ( 82. (82 7.6 (7. 3, (3 2, (2 (1 7. (7
10.
MÉTRICAS DA QUÍMICA ANALÍTICA VERDE NA AVALIAÇÃO DE MÉTODO ECOLÓGICO PARA DETERMINAÇÃO DE DEFENSIVOS AGRÍCOLAS POR CLAE-DAD CLAEDAD CLAE DAD
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Santos, Larissa P. dos
; Magalhães, Bárbara E. A. de
; Santos, Walter N. L. dos
; Oliveira, Luciana B.
; Pereira, Madson de G.
; Santana, Débora de A.
.
In recent years, the Brazilian government has authorized several agricultural pesticides, increasing the possibility of pollution of different ecosystems and the food consumed by a large part of the population. The analytical determination of pesticides can be carried out using different techniques associated with various methods. Their development and validation have been based on obtaining better reliability, meeting the requirements of environmental legislation, and adapting to the principles of Green Chemistry. Thus, a chromatographic method was developed for the separation and determination of eight agricultural pesticides (aldicarb, ametrine, atrazine, bendiocarb, carbaryl, hexazinone, methiocarb, and methomyl) in compliance with Green Chemistry criteria, using ethanol as the mobile phase, which it is an ecologically less aggressive solvent. The developed method showed efficient separation and excellent results for green analysis metrics with high scores on the Eco-scale and good presentation in the National Environmental Methods Index (NEMI), Green Analytical Procedure Index (GAPI), and Analytical Greenness Calculator Metric (AGREE) pictograms. The validation parameters indicated the linearity of the method with good precision, selectivity, and sensitivity with detection and quantification limits in the ranges of 0.50 to 0.94 mg L–1 and 0.74 to 1.81 mg L–1, respectively. years population methods reliability legislation Thus aldicarb, aldicarb (aldicarb ametrine atrazine bendiocarb carbaryl hexazinone methiocarb methomyl criteria phase solvent Ecoscale Eco scale NEMI, NEMI , (NEMI) GAPI, GAPI (GAPI) AGREE (AGREE pictograms precision selectivity 050 0 50 0.5 094 94 0.9 L1 L 1 L– 074 74 0.7 181 81 1.8 respectively (NEMI (GAPI 05 5 0. 09 9 07 7 18 8 1.
11.
Emerging challenges in bacterial resistance: prospects for the strategic use of bacteriophages resistance
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SILVA, ELOIA EMANUELLY D.
; SILVA, DEISE MARIA R.R.
; JESUS, PAMELA C. DE
; SOUZA, JESSIANE B. DE
; BARRETO, MARINA S.
; GUIMARÃES, ADRIANA G.
; MOURA, PEDRO HENRIQUE M.
; SANTOS, RONALDY S.
; SANTANA, LUCAS A.M.
; BORGES, LYSANDRO P.
.
Anais da Academia Brasileira de Ciências
- Métricas do periódico
12.
Immunoregulatory effects of soluble antigens of Leishmania sp. in human lymphocytes in vitro sp
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Silva, E. O.
; Cruz-Borges, P. F.
; Jensen, B. B.
; Santana, R. B.
; Pinheiro, F. G.
; Moura, H. S. D.
; Porto, E.
; Malheiro, A.
; Costa, A. G.
; Barcellos, J. F. M.
; Espir, T. T.
; Franco, A. M. R.
.
Resumo As manifestações clínicas da leishmaniose cutânea (LC) dependem não apenas da espécie infectante envolvida, mas também da resposta imune do indivíduo. Apesar de ainda não bem esclarecido em humanos, a sobrevivência e persistência do parasito estão relacionados ao perfil de citocinas e proliferação de células T, relacionando-se a cura ao perfil Th1, e a progressão da doença ao perfil Th2. Considerando a necessidade de estudos voltados para as espécies de maior circulação no estado do Amazonas, este trabalho teve como objetivo analisar a imunorregulação estimulada por antígenos solúveis (SLAs) de Leishmania (L.) amazonensis e Leishmania (V.) guyanensis em linfócitos humanos in vitro, afim de compreender a resposta imune de pacientes com LC. A linfoproliferação foi avaliada frente a estímulos de SLAs de L. amazonensis (100 μg/mL), SLAs de L. guyanensis (100 μg/mL) e Phytohemagglutinin (10 μg/mL) utilizando BrdU Cell Proliferation ELISA Kit após 72h de incubação. A quantificação das citocinas IL-1b, IL-6, IL-8, IL-10, IL-12 e TNF foi realizada por BD™ Cytometric Bead Array (CBA) Human Th1/Th2/Th17 Cytokine Kit. Nossos resultados demonstraram que os antígenos solúveis de L. amazonensis e L. guyanensis estimularam a linfoproliferação de PBMCs de pacientes primoinfectados com LC. Entre as citocinas dosadas, as de maiores concentrações foram de IL-6 e IL-8, demonstrando que os antígenos solúveis avaliados são capazes de induzir mecanismos regulatórios. LC (LC envolvida indivíduo T relacionandose relacionando se Th1 Th Th2 Amazonas (SLAs L (L. V. V (V. vitro 100 μg/mL, μgmL μg/mL , μg mL 10 (1 h incubação IL1b, IL1b ILb IL 1b, 1b b IL-1b IL6, IL6 6, 6 IL8, IL8 8, 8 IL-8 IL10, IL10 10, IL-10 IL12 12 IL-1 BD CBA (CBA Th1Th2Th17 ThThTh Th17 Th1/Th2/Th1 dosadas IL- regulatórios (L (V 1 ( IL1 Th1Th2Th1 Th1/Th2/Th Th1Th2Th
Abstract The clinical manifestations of cutaneous leishmaniasis (CL) depend not only on the infecting species involved, but also on the immune response of the individual. Although not yet well understood in humans, parasite survival and persistence are related to the cytokine profile and T cell proliferation, with the Th1 profile being related to cure, and the Th2 profile to disease progression. Considering the need for studies focused on the species with the highest circulation in the state of Amazonas, this study aimed to analyze the immunoregulation stimulated by soluble antigens (SLAs) of Leishmania (L.) amazonensis and Leishmania (V.) guyanensis in human lymphocytes in vitro, in order to understand the immune response of patients with CL. Lymphoproliferation was evaluated against stimuli of SLAs from L. amazonensis (100 µg/mL), SLAs from L. guyanensis (100 µg/mL) and phytohemagglutinin (10 µg/mL) using a BrdU Cell Proliferation ELISA kit after 72 h of incubation. Quantification of the cytokines IL-1b, IL-6, IL-8, IL-10, IL-12 and TNF was performed using the BD™ cytometric bead array human Th1/Th2/Th17 cytokine kit. Our results demonstrated that soluble antigens from L. amazonensis and L. guyanensis stimulated the lymphoproliferation of PBMCs from patients primo-infected with CL. Among the cytokines dosed, the highest concentrations were of IL-6 and IL-8, thus demonstrating that the soluble antigens evaluated are capable of inducing regulatory mechanisms. CL (CL involved individual humans proliferation Th cure progression Amazonas (SLAs L (L. V. V (V. vitro 100 µg/mL, µgmL µg/mL , µg mL 10 (1 7 incubation IL1b, IL1b ILb IL 1b, 1b b IL-1b IL6, IL6 6, 6 IL8, IL8 8, 8 IL-8 IL10, IL10 10, IL-10 IL12 12 IL-1 BD Th1Th2Th17 ThThTh Th17 Th1/Th2/Th1 primoinfected primo infected dosed IL- mechanisms (L (V 1 ( IL1 Th1Th2Th1 Th1/Th2/Th Th1Th2Th
13.
Arbuscular mycorrhizal fungi and water stress on the physiology and quality of Parkia platycephalic benth
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Porto, Dayara Lins
; Oliveira, Ruthanna Isabelle de
; Cunha, Jenilton Gomes da
; Araújo, Emanuel França
; Chaves, Daniela Vieira
; Boechat, Cácio Luiz
; Arauco, Adriana Miranda de Santana
.
ABSTRACT Background: The water deficit characteristic of semi-arid regions can cause negative changes in plant physiology. Arbuscular mycorrhizal fungi can meet water needs by associating with plant roots, improving seedling quality. The physiological parameters and the quality of the seedlings of Parkia platycephala Benth. were evaluated as a result of the association of isolated strains of Gigaspora rosea, Gigaspora margarita, Acaulospora koskei, Acaulospora morrowiae, intercalated strains (a mixture of the four strains mentioned above) and a control (without inoculation) under two conditions of water regime (with and without stress). Results: Artificial inoculation of AMF is a promising management technique in the production of P. platycephala Benth seedlings for improving the physiological state, increasing seedling quality, and drought resilience. Under restriction water restriction conditions, the seedlings showed lower values of net CO2 assimilation, leaf transpiration, stomatal conductance, and higher values of internal CO2 concentration. Under these conditions, the G. rosea strain stood out with the lowest rate of net CO2 assimilation (A), leaf transpiration (E), and stomatal conductance (gs) with increases of 34, 49, and 70 %, respectively, compared to the control. Conclusion: Stomatal closure is a strategy used by Parkia platycephala Benth. seedlings to prevent excessive water loss, and the Gigaspora rosea strain is responsible for mediating the best physiological conditions and better quality seedlings. Background semiarid semi arid physiology roots margarita koskei morrowiae above stress. stress . stress) Results P state resilience CO concentration G A, A , (A) E, E (E) gs (gs 34 49 7 % respectively Conclusion loss (A (E 3 4
14.
Physicochemical characterization of residual potato ( Solanum tuberosum) starch recovered from the potato chips industry in Mexico
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Montoya-Anaya, Diana G.
; Madera-Santana, Tomas J.
; Aguirre-Mancilla, Cesar L.
; Grijalva-Verdugo, Claudia
; Gonzales-Garcia, Gerardo
; Nuñez-Colín, Carlos A.
; Rodriguez-Nuñez, Jesus R.
.
Resumen El objetivo de este estudio fue evaluar las propiedades fisicoquímicas, morfológicas, estructurales y térmicas del almidón recuperado de los residuos de Solanum tuberosum de la industria de frituras en México, utilizando técnicas instrumentales avanzadas. La papa residual se recogió de una empresa productora de papas fritas y el almidón se obtuvo mediante extracción acuosa. El almidón presentó un contenido de carbohidratos de 87.79 %, proteínas (1.18 %), lípidos (0.15 %), cenizas (0.26 %) y amilosa (24.35 %). La capacidad de absorción de agua fue de 1.0163 g g-1, solubilidad (30.17 - 46.65 %), hinchamiento (2.31 - 8.69 g g-1). Los análisis térmicos mostraron una gelatinización del almidón entre 61.7 - 90 °C con una pérdida máxima de peso a 288.1 °C. La microscopía electrónica de barrido-espectroscopía dispersiva de energía (SEM-EDS) mostró partículas de 14 - 69 µm con una composición de 54.3 % de carbono, 45.4 % de oxígeno y 0.15 % de potasio. La dispersión dinámica de luz (DLS) mostró partículas de tamaño nanométrico (120 nm). La resonancia magnética nuclear (NMR) y la difracción de rayos X (XRD) exhibieron la cristalinidad del almidón como tipo B (34.73 %) y la reflectancia total atenuada por espectroscopia infrarroja transformada de Fourier (FTIR - ATR) mostró los grupos funcionales distintivos de la glucosa.
Abstract The objective of this study was to evaluate the physicochemical, morphological, structural, and thermal properties of starch recovered from Solanum tuberosum residual of chips industry in Mexico using advanced instrumental techniques. The residual potato was collected from a chips production company and the starch obtained by aqueous extraction. The starch presented a content of carbohydrates of 87.79 %, proteins (1.18 %), lipids (0.15 %), ashes (0.26 %), and amylose (24.35 %). The water absorption capacity was 1.0163 g g-1, solubility (30.17 - 46.65 %), swelling (2.31 - 8.69 g g-1). Thermal analyzes showed a starch gelatinization about 61.7 - 90 ºC with a maximum weight loss at 288.1 °C. The scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) showed particles of 14 - 69 µm with a composition of 54.3 % carbon, 45.4 % oxygen, and 0.15 % potassium. The dynamic light scattering (DLS) showed particles of nanometric size (120 nm). The nuclear magnetic resonance (NMR) and X-Ray diffraction (XRD) exhibited the crystallinity of the starch as type B (34.73 %) and the Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) showed the functional groups distinctive of the glucose.
15.
O gênero Geastrum (Geastraceae, Basidiomycota) na Mata Atlântica baiana: novas ocorrências e checklist para o Nordeste do Brasil Geastraceae, Geastraceae (Geastraceae Basidiomycota baiana
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Pires, Áureo Barbosa
; Araújo, Ruane Vasconcelos Bento de
; Ribeiro, Mateus Santana
; Silva, Bianca Denise Barbosa da
.
RESUMO Geastrum é um gênero de fungos gasteroides que se caracteriza pelo basidioma semelhante a estrelas com mecanismo de fole para liberação dos esporos. O gênero está representado por 43 espécies na região nordeste brasileira. No Estado da Bahia apenas oito espécies são conhecidas. O objetivo deste trabalho é descrever as novas ocorrências de Geastrum para Bahia. Geastrum argentinum representa o primeiro registro para o nordeste brasileiro; G. brunneocapilatum, G. entomophilum, G. lloydianum e G. triplex representam primeiro registro para a funga baiana. Descrições, foto das espécies e lista de ocorrências para o Nordeste do Brasil são apresentadas. esporos 4 brasileira conhecidas brasileiro G brunneocapilatum entomophilum baiana Descrições apresentadas
ABSTRACT Geastrum is a genus of gasteroid fungi characterized by a star-like basidiome and a bellows mechanism for releasing spores. The genus is represented by 41 species in the Brazilian northeast region. In the State of Bahia only seven species are known. The objective of this work is to describe the new occurrences of Geastrum for the State. Geastrum argentinum represents the first record for northeastern Brazil; G. brunneocapilatum, G. entomophilum, G. lloydianum and G. triplex represent the first record for Bahia State funga. Descriptions, photos of the species and checklist for northeastern Brazil are presented. starlike star like spores 4 region known G brunneocapilatum entomophilum funga Descriptions presented
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