The inhibition of the fatty acid amide hydrolase (FAAH), an endocannabinoid system component, emerged as a potentially new therapeutic target for a range of clinical disorders such as acute and chronic pain. Some α-ketoheterocycle derivatives demonstrated interesting analgesic and anti-inflammatory activities in vitro. Ligand-Based Drug Design techniques such as knowledge graph convolutional networks (kGCN) and hologram quantitative structure-activity relationship (HQSAR) using α-ketoheterocycle derivatives from five different datasets were generated to discover the relation between the chemical structures and the inhibition activity. Meanwhile, structure-based drug design simulations as interaction fields (MIF), molecular docking, and ligand sites studies (LSI) from FAAH were performed using Autogrid software and FTmap/FTsite servers. The results of both studies were merged to propose predictive models. The resulting kGCN model demonstrated adequate accuracy area under the curve by receiver operating characteristic (AUC-ROC 0.7922). From contribution maps of the Ligand-Based Drug Design (LBDD) models and the generated probes using MIF and LSI, it was observed that the oxazole ring, the ketone group, and the apolar chain present in the structures of the inhibitors are important, besides the evidence of the Cys269 and Val270 residues importance for the potential interaction, confirmed by carried docking studies. These fragments and structural information can be used to carry out new FAAH potential inhibitors studies and report kGCN as an accurate classification technique. FAAH, , (FAAH) component pain αketoheterocycle α ketoheterocycle antiinflammatory anti inflammatory vitro LigandBased Ligand Based (kGCN structureactivity structure activity HQSAR (HQSAR Meanwhile structurebased based MIF, (MIF) LSI (LSI FTmapFTsite FTmap FTsite servers AUCROC AUC ROC 0.7922. 07922 0.7922 . 0 7922 0.7922) LBDD (LBDD ring group important Cys Cys26 Val Val27 technique (FAAH (MIF 0792 0.792 792 Cys2 Val2 079 0.79 79 07 0.7 7 0.

ABSTRACT Giant hepatic hemangiomas are occasional in patients with cirrhosis. It remains a challenge to decide on the need for treatment and choose the most appropriate intervention. A 62-year-old woman was recently diagnosed with cirrhosis and complained of upper abdominal fullness, reduction in oral food intake, and weight loss of 6 kg over the last three years. Upper digestive endoscopy evidenced thin-caliber esophageal varices and significant extrinsic compression of the lesser gastric curvature. Abdominal computed tomography revealed an exophytic tumor in the left hepatic lobe, measuring 11.5 cm, which had progressive centripetal contrast enhancement from the arterial phase, compatible with hepatic hemangioma. Serum tumor markers were negative, and her liver function was unimpaired. The patient underwent surgical resection (non-anatomical hepatectomy of segments II and III) which had no immediate complications, and the histopathological evaluation confirmed cavernous hepatic hemangioma. Two weeks later, she was admitted to the emergency room with jaundice, signs of hepatic encephalopathy, and moderate ascites, and was further diagnosed with secondary bacterial peritonitis. As no perforations, abscesses, or fistulas were observed on subsequent imaging tests, clinical management was successfully carried out. This case highlights that giant hepatic hemangiomas may be symptomatic and warrant treatment. In the setting of cirrhosis and portal hypertension, physicians should be aware of the risk of hepatic decompensation following surgical resection, even in patients with Child-Pugh class A. intervention 62yearold yearold 62 year old fullness intake years thincaliber thin caliber curvature lobe 115 11 5 11. cm phase hemangioma negative unimpaired nonanatomical non anatomical III complications later jaundice encephalopathy ascites peritonitis perforations abscesses tests out hypertension ChildPugh Child Pugh 1

Short-chain chiral esters are important constituents in natural fruit flavors, as well as in food and pharmaceutical industries. Different chemical and bio-catalytic routes are used for their synthesis, but the use of simultaneous techniques has not been much explored, as combinatorial synthesis and bio-catalysis in continuous-flow systems. Here, the objective was to synthesize chiral esters typically found in fruit flavors, to obtain chemical libraries with high conversion and enantiomeric excess, using combinations of these three techniques. Combinatorial synthesis in continuous-flow coil-type reactors with fix bed was used in the esterification of the secondary alcohols 2-butanol (1a), 2-pentanol (1b), 3-hexanol (1c), 2-heptanol (1d) and 2-octanol (1e) with acetic (2) and propanoic (3) acids. A surface-response method was applied to optimize the reaction conditions. Conversions were above 88% after 60 min for the reaction with chemical catalysts in continuous-flow reactors compared to 85%, but after 120 min, when conventional batch processes were used. By applying a biocatalyst, a chemical library of R acetates was prepared, with conversions over 48% after 48 h for batch and 40% after only 70 min for continuous flow reactions. It was observed that both enzymatic and homogeneous chemical catalysis showed to be viable for the chiral esters. Shortchain Short chain flavors industries biocatalytic bio catalytic explored biocatalysis continuousflow systems Here excess coiltype coil type 2butanol butanol 2 1a, 1a , (1a) 2pentanol pentanol 1b, 1b b (1b) 3hexanol hexanol 3 1c, 1c c (1c) 2heptanol heptanol 1d d (1d 2octanol octanol 1e e (1e (2 (3 acids surfaceresponse surface response conditions 88 6 85 85% 12 biocatalyst prepared 4 40 7 reactions (1a (1b (1c ( 8 1

Solid tumors are frequently affected by the presence of hypoxic cells, originating anomalous and disordered cell growth. This subpopulation presents resistance to conventional chemotherapy agents and radiotherapy, due to the difficulties of reaching the target region, the permanence in the G0 phase of cell cycle and the low local concentration of oxygen. The use of hypoxia activated prodrugs becomes, in this scenario, an effective and selective alternative in cancer therapy, though not yet available for clinical use in solid tumor treatment. Twenty years after the publication of the paper intitled “Agentes Antineoplásicos Biorredutíveis: Uma Nova Alternativa para o Tratamento de Tumores Sólidos” in the Química Nova journal (2002), the current essay aims to identify the advances in the pre-clinical and clinical studies regarding the development of new hypoxia-directed drugs and the improvement of the molecules previously studied. Moreover, to clarify the main challenges for the implementation and success of this drug class, in order to identify the hindrances for the preparation of a bioavailable, safe and selective drug. cells growth radiotherapy region G oxygen becomes scenario therapy treatment Agentes Biorredutíveis Sólidos 2002, 2002 , (2002) preclinical pre hypoxiadirected directed studied Moreover class bioavailable 200 (2002 20 (200 2 (20 (2 (

Abstract Plants used in traditional medicine offer an affordable new alternative in tissue repair therapy. This study aimed to evaluate the effectiveness of the 5% Rhizophora mangle cream compared to the 5% dexpanthenol cream in healing open surgical wounds on the upper eyelid. A total of 18 patients were submitted to the experiment and divided into 2 groups with 9 patients each who used topically and daily 5% dexpanthenol cream (control group) or 5% R.mangle cream (intervention group) for 7 days. Clinical, morphometric and histomorphometric analyses of wounds and surgical procedures for skin removal were performed. In the morphometric analysis, all wounds treated with R.mangle and dexpanthenol creams showed complete macroscopic scars, without inflammatory signs and infection free. The skin hydration values in pre and post application periods of the cream were 43.82 ± 43.93 and 62.12 ± 67.40 respectively. The histomorphometric study showed lower values of epithelium distance in R. mangle group and higher in dexpanthenol group with significant difference between groups (p < 0.05). The R.mangle 5% cream proved to be effective in healing wounds of human upper eyelid skin with a significant improvement in epithelization compared to dexpanthenol 5% cream. therapy 5 1 control Rmangle R intervention days Clinical performed analysis scars free 4382 43 82 43.8 4393 93 43.9 6212 62 12 62.1 6740 67 40 67.4 respectively p 0.05. 005 0.05 . 0 05 0.05) 438 4 8 43. 439 621 6 62. 674 67. 00 0.0 0.

ABSTRACT The horizontal transmission of the entomopathogenic fungus Beauveria bassiana Unioeste 76 strain among adults of Euschistus heros was evaluated in the laboratory and greenhouse using sporulated cadavers and live insects contaminated as a source of inoculate. Adult insects in the laboratory bioassay either received treatments with dry conidia or were sprayed with an oily dispersion formulation, and the treatments were comprised of 10, 30, or 50% of contaminated insects. For the greenhouse bioassay, nylon cages were prepared, with soybean plants grown in pots. A total of 20 healthy brown stink bugs were released along with one or three sporulated cadavers of brown stink bugs, attached to the plant stem. In the bioassay with live insects, 20 stink bugs were released, and the treatments were comprised of 10, 30, or 50% contaminated insects (2:18, 6:14, and 10:10, fungus-treated insects : healthy insects). In all experiments, the control group was comprised only of healthy insects. Dead stink bugs were daily disinfested and transferred to a wet chamber for confirmation of death by fungus. Confirmed mortality was observed, which shows the occurrence of horizontal transmission. Mortality was higher when more contaminated insects were used in the population, in most treatments. The occurrence of transmission in the laboratory and the greenhouse emphasizes the potential of the Unioeste 76 strain as mortality agent and as an agent for maintaining the inoculum potential for the brown stink bug. 7 inoculate formulation 10 30 50 prepared pots 2 stem 218, 218 18, 18 (2:18 614, 614 6 14, 14 6:14 1010, 1010 10:10 fungustreated treated . insects) experiments observed population bug 1 3 5 21 (2:1 61 6:1 101 10:1 (2: 6: 10: (2 (

Abstract The use of biomass for energy production constitutes a promising strategy that warrants the search for new sources of biomass. Elephant grass has been gaining notoriety due to its high dry matter yield and rapid growth. The present study was carried out to quantify the genetic divergence of nine elephant grass half-sib families in order to identify genotypes with greater genetic divergence and productive potential for hybridization, using the hierarchical clustering methodology based on principal components. Half-sib families were generated using genotypes from the Active Germplasm Bank of Elephant Grass. The experiment was laid out in a randomized-block design with nine half-sib families, three replicates, and eight plants per plot. A total of 216 genotypes of elephant grass were evaluated. Principal component (PC), biplot, and hierarchical clustering analyses for diversity estimation were conducted using R software. The first two PCs of biplot analysis accounted for 64% of the cumulative variation. Dry matter yield was the most important trait for genotype discrimination (0.89), followed by plant height (0.67) and stem diameter (0.61) in PC1. In this analysis, the distances between accessions were considered and there were no family links, which indicates the existence of wide variability within the evaluated families, since genotypes belonging to the same family were not grouped together, but rather distributed into different groups. Crosses between genotypes of group three and genotypes of groups one and two are recommended for the development of high-yielding genotypes when aiming at energy production. growth halfsib half sib hybridization components Halfsib Half Grass randomizedblock randomized block replicates plot 21 PC, PC , (PC) software 64 variation 0.89, 089 0.89 0 89 (0.89) 0.67 067 67 (0.67 0.61 061 61 (0.61 PC1 links together highyielding yielding 2 (PC 6 08 0.8 8 (0.89 0.6 06 (0.6 0. (0.8 (0. (0 (

Resumo A utilização de biomassa para a produção de energia representa uma estratégia promissora, justificando a busca por novas fontes de biomassa. O capim-elefante tem ganhado destaque devido ao seu elevado rendimento de matéria seca e rápido crescimento. Este estudo teve como objetivo quantificar a divergência genética entre novas famílias de meio-irmãos de capim-elefante, com a finalidade de identificar genótipos com maior divergência genética e potencial produtivo para hibridização. Utilizou-se uma metodologia de agrupamento hierárquico baseada em componentes principais. As famílias de meio-irmãos foram obtidas a partir de genótipos do Banco Ativo de Germoplasma de Capim-Elefante. O experimento foi conduzido em delineamento de blocos casualizados, com nove famílias de meio-irmãos, três repetições e oito plantas por parcela, totalizando a avaliação de 216 genótipos de capim-elefante. Foram realizadas análises de componentes principais (PC), biplot e agrupamento hierárquico para estimar a diversidade, utilizando o software R. Os dois primeiros PCs da análise biplot explicaram 64% da variação total. A produtividade de matéria seca foi a característica mais relevante para a discriminação genotípica (0,89), seguida pela altura da planta (0,67) e pelo diâmetro do caule (0,61) no PC1. Nesta análise, foram consideradas as distâncias entre os acessos, sem considerar parentesco, o que indica uma variabilidade significativa dentro das famílias avaliadas, uma vez que os genótipos pertencentes à mesma família não foram agrupados, mas sim distribuídos em grupos distintos. promissora capimelefante capim elefante crescimento meioirmãos meio irmãos capimelefante, elefante, hibridização Utilizouse Utilizou se CapimElefante. CapimElefante Capim Elefante. Elefante Capim-Elefante casualizados meioirmãos, irmãos, parcela 21 capimelefante. elefante. PC, PC , (PC) diversidade R 64 total 0,89, 089 0,89 0 89 (0,89) 0,67 067 67 (0,67 0,61 061 61 (0,61 PC1 acessos parentesco avaliadas agrupados distintos 2 (PC 6 08 0,8 8 (0,89 0,6 06 (0,6 0, (0,8 (0, (0 (

Abstract Background: Arboviral diseases are a group of infectious diseases caused by viruses transmitted by arthropods, mainly mosquitoes. These diseases, such as those caused by the dengue (DENV), Zika (ZIKV), chikungunya (CHIKV), and yellow fever (YFV) viruses, have a significant impact worldwide. In this context, entomological surveillance plays a crucial role in the control and prevention of arboviruses by providing essential information on the presence, distribution, and activity of vector mosquitoes. Based on entomological surveillance, transovarian transmission provides information regarding the maintenance and dissemination of arboviruses. The objective of this study was to detect these arboviruses in Goiânia, Goiás, and analyze the occurrence of transovarian transmission. Methods: Aedes aegypti eggs were collected from different regions of Goiânia and cultivated under controlled laboratory conditions until the emergence of adult mosquitoes. Adult females were grouped into pools containing their heads and thoraxes. These pools were subsequently evaluated using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay. Results: A total of 157 pools (N=1570) were analyzed, with two pools testing positive for CHIKV and one pool testing positive for ZIKV, indicating that the offspring resulting from transovarian transmission are potentially infectious. Conclusions: In summary, the demonstration of the vertical transmission mechanisms of CHIKV and ZIKV in A. aegypti serves as an alert to health authorities, as these diseases are still underreported, and their primary urban vector has likely acquired this capacity, contributing to the dissemination of these infections. Background arthropods mosquitoes DENV, DENV , (DENV) (ZIKV) CHIKV, (CHIKV) YFV (YFV worldwide context presence distribution Goiás Methods thoraxes reversetranscription reverse transcription RTqPCR RT qPCR (RT-qPCR assay Results 15 N=1570 N1570 N 1570 (N=1570 analyzed Conclusions summary authorities underreported capacity infections (DENV (ZIKV (CHIKV 1 N=157 N157 (N=157 N=15 N15 (N=15 N=1 N1 (N=1 N= (N= (N

The present study optimized the extraction process of bioactive compounds present in ginger (Zingiber officinale) dried at 80 °C, using ethanol:water 70:30 (v/v) as solvent. The extracts were evaluated for antioxidant activity by the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reduction methods and by the chelating activity of FeII ions. It was found that the extraction condition with a temperature of 60 °C and an extraction time of 12 h showed better responses to the tests. Then, the characterization of the compounds was carried out by mass spectrometry and thermal analysis (thermogravimetric (TG), differential thermal analysis (DTA), and differential scanning calorimetry (DSC)), identifying that the main compounds of ginger were gingerols and shogaols, being confirmed by the intensities and characteristics of the thermal graphs. The inhibition of the enzyme acetylcholinesterase (AChE) was evaluated using the Ellman test, which did not show an inhibitory action. Regarding cytotoxic activity, the free extract and encapsulated in liposomes were tested, showing antiproliferative effect at different concentrations for human kidney tumor cells (786-0), liver cells (HUH7.5), and Macaca mullata normal kidney cells (LLC-MK2). Given the results obtained, ginger presents itself as a renewable source of bioactive compounds and can be indicated for applications in the pharmaceutical industry. Zingiber officinale 8 C ethanolwater ethanol water 7030 70 30 70:3 v/v vv v (v/v solvent 2,2azinobis3ethylbenzothiazoline6sulfonic 22azinobis3ethylbenzothiazoline6sulfonic azinobisethylbenzothiazolinesulfonic 2,2 azino bis 3 ethylbenzothiazoline 6 sulfonic 2 ABTS (ABTS 2,2diphenyl1picrylhydrazyl 22diphenyl1picrylhydrazyl diphenylpicrylhydrazyl diphenyl 1 picrylhydrazyl DPPH (DPPH ions tests Then thermogravimetric TG, TG , (TG) DTA, DTA (DTA) DSC, DSC (DSC)) shogaols graphs AChE (AChE test action tested 7860, 7860 786 0 (786-0) HUH7.5, HUH75 HUH HUH7.5 HUH7 5 (HUH7.5) LLCMK2. LLCMK2 LLCMK LLC MK2 . MK (LLC-MK2) obtained industry 703 7 70: 2azinobis3ethylbenzothiazoline6sulfonic azinobis 22 2, 2diphenyl1picrylhydrazyl (TG (DTA (DSC) 78 (786-0 HUH7. (HUH7.5 (LLC-MK2 (DSC (786- (HUH7. (LLC-MK (786 (HUH7 (78 (HUH (7 (

ABSTRACT Resistant genotypes are a valuable tool in integrated pest management programs of insect pests of stored grains. Here, we screened 90 Brazilian common bean genotypes to select promising sources of resistance to Acanthoscelides obtectus. Thus, bean samples were infested with newly emerged insects, and the number of eggs, emerged insects, developmental time, and larval viability were assessed in a no-choice test. Genotypes IPR Garça, CHIR 13, IAC Harmonia, CHIR 20, IAC Boreal, and CHIP 312 showed low oviposition. Genotypes IPR Quero-Quero, BRS Notável, CHIC 61 - Cariocão, BRS Ametista, IPR Uirapuru, IAC Imperador, Avaluna, CHP 04-241A-212 - Guapo B. × Uirapuru, CHIB 06, SCS 206 - Potência, CHC 01-175-1 - Campeiro × IAC Tibatã, CHP 01-182-48 - Uirapuru × Campeiro, UEM 266, CHC 04-233-2 - Siriri × Horizonte, CHV 17-64, SCS 205 - Riqueza, CHP 01-238-80 - MN 13337, SM 1510 - preto, BRS Radiante, CHIB 18, CHIR 14, CAV 17 EFM 008, CHIR 04, IPR Campos Gerais, CHV 05-268-02, LP 13-624 - preto, Linhagem 110 - IAC, CHP 01-182-12 - Uirapuru × Campeiro, LP 13-84 - carioca, BRS Esteio, CHV 17-635, CHB 15-518, Arcelina 1, IPR Tuiuiu, SCS 204 - Predileto, TB - 17-02, BRS Estilo, SM 0511 - cavalo preto, Pérola, IPR Tangará, and CNFRj - 15411 reduced oviposition, the number of emerged adults, and larval viability, suggesting antixenosis and/or antibiosis. Thus, these genotypes are promising sources of resistance factors and can be used in breeding programs to obtain lines of common beans resistant to A. obtectus. grains Here 9 obtectus Thus insects eggs time nochoice no choice test Garça 13 Harmonia 20 Boreal 31 oviposition QueroQuero, QueroQuero Quero Quero, Quero-Quero Notável 6 Cariocão Ametista Imperador Avaluna 04241A212 A 04 241A 212 04-241A-21 B 06 Potência 011751 01 175 1 01-175- Tibatã 0118248 182 48 01-182-4 266 042332 233 2 04-233- Horizonte 1764, 1764 64, 64 17-64 Riqueza 0123880 238 80 01-238-8 13337 151 preto Radiante 18 14 008 Gerais 0526802, 0526802 05 268 02, 02 05-268-02 13624 624 13-62 11 0118212 12 01-182-1 1384 84 13-8 carioca Esteio 17635, 17635 635, 635 17-635 15518, 15518 15 518, 518 15-518 Tuiuiu Predileto 1702, 1702 17-02 Estilo 051 Pérola Tangará 1541 adults andor or antibiosis 3 04241A21 0 21 04-241A-2 01175 01-175 011824 4 01-182- 26 04233 23 04-233 176 17-6 012388 8 01-238- 1333 00 052680 05-268-0 1362 62 13-6 011821 138 13- 1763 63 17-63 1551 51 15-51 170 17-0 154 04241A2 04-241A- 0117 01-17 01182 01-182 0423 04-23 17- 01238 01-238 133 05268 05-268- 136 155 5 15-5 04241A 04-241A 011 01-1 0118 01-18 042 04-2 0123 01-23 0526 05-268 15- 01- 04- 012 01-2 052 05-26 05-2 05-

ABSTRACT Blood samples from 200 female dogs divided into 6 groups (diestrus, mucometra, pyometra) were evaluated, with the pyometra group categorized according to the ASA classification (American Society of Anesthesiologists), that is, from ASA II to V, totaling six groups. Aiming to analyze the acute phase proteins (APP), hematological and serum biochemical profiles of the female dogs in the study, establishing the differential diagnosis and prognosis according to the group. The SDS-PAGE method was used for protein fractionation, complete blood count using an automated hematological analyzer and histogram, biochemical tests performed using a semi-automatic spectrophotometer and measurement of serum concentrations of sodium and ionic calcium using the selective ion method. The results showed that female dogs with pyometra categorized in the ASA II to V classification revealed biochemical alterations between the study groups, as well as the acute phase proteins, presented variations according to the systemic involvement, degree of inflammatory response and ASA classification of the female dogs with pyometra, in which ceruloplasmin (p=0.07) and transferrin (p=0.07) did not show statistical significance, but showed an increase in their concentration according to the inflammatory evolution, albumin (p<0.0001), IgGCP (p <0.0001), haptoglobin (p<0.0001), alpha acid glycoprotein (p<0.0001), IgGCL (p<0.0001) and the one identified by its atomic weight 23,000 Da (p=0.0031), enabling the assessment of the acute phase response and aiding in the early detection of the systemic inflammatory response and better therapeutic guidance for the patient. 20 diestrus, diestrus (diestrus mucometra evaluated American Anesthesiologists, Anesthesiologists , Anesthesiologists) is APP, APP (APP) SDSPAGE SDS PAGE fractionation histogram semiautomatic semi automatic involvement p=0.07 p007 p 0 07 (p=0.07 significance evolution p<0.0001, p00001 p<0.0001 0001 <0.0001, 00001 <0.0001 <0.0001) (p<0.0001 23000 23 000 23,00 p=0.0031, p00031 p=0.0031 0031 (p=0.0031) patient 2 (APP p=0.0 p00 (p=0.0 p0000 p<0.000 0000 <0.000 (p<0.000 2300 00 23,0 p0003 p=0.003 003 (p=0.0031 p=0. p0 (p=0. p000 p<0.00 <0.00 (p<0.00 230 23, p=0.00 (p=0.003 p=0 (p=0 p<0.0 <0.0 (p<0.0 (p=0.00 p= (p= p<0. <0. (p<0. p<0 <0 (p<0 p< < (p<

RESUMO Foram avaliadas amostras de sangue de 200 cadelas, divididas em seis grupos (diestro, mucometra, piometra), sendo o grupo piometra categorizado de acordo com a classificação ASA (American Society of Anesthesiologists), ou seja, de ASA II a V, totalizando seis grupos. O objetivo deste estudo foi analisar as proteínas de fase aguda (APP), os perfis hematológicos e bioquímicos séricos das cadelas do estudo, estabelecendo-se o diagnóstico diferencial e o prognóstico de acordo com o grupo. O método SDS-PAGE foi utilizado para fracionamento de proteínas. Foi feito hemograma completo com analisador hematológico automático e histograma, exames bioquímicos com espectrofotômetro semiautomático e medição das concentrações séricas de sódio e cálcio iônico pelo método de íons seletivos. Os resultados mostraram que cadelas com piometra categorizadas na classificação ASA II a V revelaram alterações bioquímicas entre os grupos de estudo, assim como as proteínas de fase aguda apresentaram variações de acordo com o envolvimento sistêmico, o grau de resposta inflamatória e a classificação ASA das cadelas com piometra, nas quais a ceruloplasmina (P=0,07) e a transferrina (P=0,07) não apresentaram significância estatística, mas apresentaram aumento em sua concentração conforme a evolução inflamatória, albumina (P<0,0001), IgGCP (P<0,0001 ), haptoglobina (P<0,0001), glicoproteína alfa ácida (P<0,0001), IgGCL (P<0,0001) e aquela identificada pelo seu peso atômico 23.000 Da (P=0,0031), o que possibilitou a avaliação da resposta de fase aguda e auxiliou na detecção precoce da resposta inflamatória sistêmica e na melhor orientação terapêutica para o paciente. 20 diestro, diestro (diestro mucometra , piometra) American Anesthesiologists, Anesthesiologists Anesthesiologists) seja APP, APP (APP) estabelecendose estabelecendo se SDSPAGE SDS PAGE histograma seletivos sistêmico P=0,07 P007 P 0 07 (P=0,07 estatística P<0,0001, P00001 P<0,0001 0001 (P<0,000 ) 23000 23 000 23.00 P=0,0031, P00031 P=0,0031 0031 (P=0,0031) paciente 2 (APP P=0,0 P00 (P=0,0 P0000 P<0,000 (P<0,00 2300 00 23.0 P0003 P=0,003 003 (P=0,0031 P=0, P0 (P=0, P000 P<0,00 (P<0,0 230 23. P=0,00 (P=0,003 P=0 (P=0 P<0,0 (P<0, (P=0,00 P= (P= P<0, (P<0 (P P<0 (P< P<

ABSTRACT Aspergillosis is a fungal disease with high morbidity and mortality in wild and exotic bird species. The aim of this report is to describe a case of acute aspergillosis caused by Aspergillus fumigatus in a young African grey parrot (Psittacus erithacus). A three-month-old male African grey parrot from a commercial breeding site presented serous nasal discharge, inappetence, and wheezing on pulmonary auscultation. The parrot died three days after the onset of clinical signs. Postmortem evaluation demonstrated multiple smooth gray plaques in air sacs and left lung with dark red areas and adhesions to the ribs. Microscopically, there were intralesional hyphae and conidiophores in the lungs, air sacs, and ribs, which were associated with pneumonia, airsacculitis, and osteomyelitis, respectively. DNA samples were extracted from paraffinized tissues and subjected to PCR targeting the ITS-2 region, followed by sequencing, which yielded a sequence with 100% coverage and 100% identity to Aspergillus fumigatus sequences. Although A. fumigatus infection is quite common in birds, a particular aspect of interest in this case was the finding of conidiophores in the bone marrow, which may occur in birds due to air circulation through pneumatic bones. species Psittacus erithacus. erithacus . erithacus) threemonthold month old discharge inappetence auscultation signs ribs Microscopically lungs pneumonia airsacculitis osteomyelitis respectively ITS2 ITS 2 ITS- region sequencing 100 sequences marrow bones 10 1

RESUMO Aspergilose é uma doença fúngica com alta morbidade e mortalidade em aves domésticas e exóticas. O objetivo deste relato foi descrever um caso de aspergilose aguda causada por Aspergillus fumigatus em um papagaio-cinzento (Psittacus erithacus). Um papagaio-cinzento de três meses de idade de um criatório comercial apresentou secreção nasal serosa, inapetência e estertores pulmonares. O papagaio morreu três dias após o início dos sinais clínicos. Exame post mortem demonstrou múltiplas placas acinzentadas nos sacos aéreos, e o pulmão esquerdo apresentava áreas vermelho-escuras aderidas às costelas. Microscopicamente, havia hifas e conidióforos intralesionais nos pulmões, nos sacos aéreos e nas costelas, associados à pneumonia, aerossaculite e osteomielite, respectivamente. Amostras de DNA extraídas de tecidos parafinizados e submetidas à PCR para amplificação da região ITS-2, seguida de sequenciamento, resultaram em sequência com 100% de cobertura e 100% de identidade com sequências de Aspergillus fumigatus. Embora a infecção por A. fumigatus seja comum em aves, um aspecto de particular interesse neste caso foi o achado de conidióforos na medula óssea, que pode ocorrer em aves devido à circulação de ar em ossos pneumáticos. exóticas papagaiocinzento cinzento Psittacus erithacus. erithacus . erithacus) serosa pulmonares clínicos vermelhoescuras vermelho escuras costelas Microscopicamente pulmões pneumonia osteomielite respectivamente ITS2, ITS2 ITS 2, 2 ITS-2 sequenciamento 100 A óssea pneumáticos ITS- 10 1

Abstract Objectives The aim of this study was to explore the differences in the pattern of allergen sensitization in CR individuals without or with asthma, according to asthma severity. Methods A total of 1066 adults were evaluated. Asthma and chronic⁄allergic rhinits were identified by specialists, questionnaries and skin-prick test. The phenotypic characterization was avaliable from skin-prick test to an aeroallergen extended panel, total IgE and pulmonary function. Using questionnaires and clinical evaluation, participants were classified into the groups: chronic rhinitis alone (CRA) and chronic rhinitis + asthma, the latter subdivided into CR + mild asthma (CRMA) and CR + moderate to severe asthma (CRMSA). Aerollergen sensitization was defined by a positive prick test to one or more allergens associated with nasal symptoms and/or asthma. The association between CR and asthma was evaluated by multivariable logistic regression. The evidence of effect modification of pattern of sensitization in CR on the association with asthma severity and outcomes was examined by introducing interactions terms in the logistic regression models adjusting for confounders. Results Frequency of sensitization to aeroallergens was higher in association with asthma in comparison to CRA (CRMA 70.4%; CRMSA 65.0%; CRA 47.0%; p= 0.000). Similarly, the presence of asthma was associated to aeroallergen multiple sensitization (51.5%) (OR = 2.10, 95% CI 1.27-3.50). Additionally, the sensitization to mites, cockroaches, animal epithelium, grasses, and molds, were higher in asthma (56.8%, 24.3%, 12%, 7.13% and 10.3%, respectively). Sensitization to Alternaria alternata, Cladosporium herbarum and dog epithelium was exclusive in asthma groups. A concomitant asthma diagnosis was directly associated with a positive allergen sensitization at least one allergen (62.7%, OR = 2.45, 95% CI 1.80-3.34) and polissensitization (51.5%, OR = 2.10, 95% CI 1.27-3.50). Conclusion Asthma is associated with multiple allergen sensitization among patients with CR. Some unique profiles of aeroallergen sensitization were observed in patients with CR and asthma. Nevertheless, no difference was found in the sensitization in relation to asthma severity, which suggest atopy is not the main underlying mechanism for asthma severity among patients with CR. Level of evidence: Level 3. 106 chronicallergic allergic specialists skinprick skin panel function evaluation groups (CRA CRMA CRMSA. . (CRMSA) andor confounders 70.4% 704 70 4 65.0% 650 65 0 47.0% 470 47 p 0.000. 0000 0.000 000 0.000) Similarly 51.5% 515 51 5 (51.5% 210 2 10 2.10 95 1.273.50. 127350 1.27 3.50 1 27 3 50 1.27-3.50) Additionally mites cockroaches grasses molds 56.8%, 568 56 8 (56.8% 243 24 24.3% 12 12% 713 7 13 7.13 103 10.3% respectively. respectively respectively) alternata 62.7%, 627 62 (62.7% 245 45 2.45 1.803.34 180334 1.80 3.34 80 34 1.80-3.34 51.5%, Nevertheless (CRMSA 70.4 65.0 6 47.0 0.00 00 51.5 (51.5 21 2.1 9 273 1.273.50 12735 127 1.2 350 3.5 1.27-3.50 56.8% (56.8 24.3 71 7.1 10.3 62.7% (62.7 2.4 803 1.803.3 18033 180 1.8 334 3.3 1.80-3.3 70. 65. 47. 0.0 51. (51. 2. 1.273.5 1273 1. 35 1.27-3.5 56.8 (56. 24. 7. 10. 62.7 (62. 1.803. 1803 18 33 1.80-3. 0. (51 1.273. 1.27-3. 56. (56 62. (62 1.803 1.80-3 (5 1.273 1.27-3 (6 1.80- ( 1.27-

Abstract In this study, the Box-Behnken experimental planning was used to optimize the extraction of polysaccharides from the cell wall of Rhizopus microspore var. oligosporus, with analysis of the quantitative effects of parameters pH, temperature and extraction time for polysaccharide yield. The optimal conditions for extraction were determined by the regression equation and evaluation of the response surface graphs, which indicated: pH 13, temperature of 120ºC and time of 60 min, with maximum yield around 18.5%. Fourier transform infrared spectroscopy analysis indicated typical polysaccharide signals. Nuclear magnetic resonance spectroscopy and monosaccharide analysis indicated a β(1,3) β(1,6) glucogalactan. The polysaccharide exhibited an average molecular weight of 120 kDa and a polymerization degree of 741. Antioxidant assays in vitro revealed the potential of polysaccharide in elimination of ABTS+ radical and hydroxyl radicals. EC50 values for free radical elimination were 7.69 and 17.8 mg/mL, for ABTS+ and hydroxyls, respectively. The polysaccharides showed potential for α-amylase inhibition with an EC50 of 1.66 mg/mL. The results suggest that β(1,3) β(1,6) glucogalactan from Rhizopus microsporus var. oligosporus can be used in biotechnological applications. study BoxBehnken Box Behnken var graphs 13 ºC 6 min 185 18 5 18.5% signals β1,3 β13 β 1,3 1 3 β(1,3 β1,6 β16 1,6 β(1,6 12 741 ABTS radicals EC EC5 769 7 69 7.6 178 17 8 17. mgmL mg mL mg/mL hydroxyls respectively αamylase α amylase 166 66 1.6 applications 18.5 β1 β1, 1, β(1, 16 74 76 7. 1. 18. β(1 β(

This academic article investigates the performance of dye-sensitized solar cells (DSSCs), which are affected by various defects like charge recombination, high impedance, and low power conversion efficiency due to complex physicochemical phenomena. The study explores different materials and processes to enhance DSSC performance, with a focus on photoanode development. Tin dioxide, tin chloride II dihydrate, copper oxide, boric acid, and sodium phosphate are utilized to fabricate the photoanodes. A thermal treatment at 450°C for 30 minutes precedes dye assembly on the photoanode surface. Results show that the tin chloride II dihydrate-modified photoanode outperforms others, achieving higher open-circuit voltage (0.50V), current density (9.58 mAcm-2), fill factor (0.37), and power conversion efficiency (1.77%). The study suggests that tin salt particles positively impact voltage, current, electron transport, and charge separation, enhancing overall cell performance. Charge recombination significantly affects power conversion efficiency and impedance, but accurately quantifying it remains challenging. Lower recombination levels favor efficiency, while higher levels reduce it. dyesensitized sensitized DSSCs, DSSCs , (DSSCs) impedance phenomena development dioxide dihydrate oxide acid photoanodes 450C C 450 3 surface dihydratemodified modified others opencircuit open circuit 0.50V, 050V V 0.50V 0 50V (0.50V) 9.58 958 9 58 (9.5 mAcm2, mAcm2 mAcm 2 mAcm-2) 0.37, 037 0.37 37 (0.37) 1.77%. 177 1.77% . 1 77 (1.77%) transport separation challenging (DSSCs 45 (0.50V 9.5 95 5 (9. mAcm-2 03 0.3 (0.37 17 1.77 7 (1.77% 4 9. (9 mAcm- 0. (0.3 1.7 (1.77 ( (0. 1. (1.7 (0 (1. (1