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Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
Slipinski, Adam
Linzmeier, Adelita M.
Calor, Adolfo R.
Garda, Adrian A.
Kury, Adriano B.
Fernandes, Agatha C.S.
Agudo-Padrón, Aisur I.
Akama, Alberto
Silva Neto, Alberto M. da
Burbano, Alejandro L.
Menezes, Aleksandra
Pereira-Colavite, Alessandre
Anichtchenko, Alexander
Lees, Alexander C.
Bezerra, Alexandra M.R.
Domahovski, Alexandre C.
Pimenta, Alexandre D.
Aleixo, Alexandre L.P.
Marceniuk, Alexandre P.
Paula, Alexandre S. de
Somavilla, Alexandre
Specht, Alexandre
Camargo, Alexssandro
Newton, Alfred F.
Silva, Aline A.S. da
Santos, Aline B. dos
Tassi, Aline D.
Aragão, Allan C.
Santos, Allan P.M.
Migotto, Alvaro E.
Mendes, Amanda C.
Cunha, Amanda
Chagas Júnior, Amazonas
Sousa, Ana A.T. de
Pavan, Ana C.
Almeida, Ana C.S.
Peronti, Ana L.B.G.
Henriques-Oliveira, Ana L.
Prudente, Ana L.
Tourinho, Ana L.
Pes, Ana M.O.
Carmignotto, Ana P.
Wengrat, Ana P.G. da Silva
Dornellas, Ana P.S.
Molin, Anamaria Dal
Puker, Anderson
Morandini, André C.
Ferreira, André da S.
Martins, André L.
Esteves, André M.
Fernandes, André S.
Roza, André S.
Köhler, Andreas
Paladini, Andressa
Andrade, Andrey J. de
Pinto, Ângelo P.
Salles, Anna C. de A.
Gondim, Anne I.
Amaral, Antonia C.Z.
Rondón, Antonio A.A.
Brescovit, Antonio
Lofego, Antônio C.
Marques, Antonio C.
Macedo, Antonio
Andriolo, Artur
Henriques, Augusto L.
Ferreira Júnior, Augusto L.
Lima, Aurino F. de
Barros, Ávyla R. de A.
Brito, Ayrton do R.
Romera, Bárbara L.V.
Vasconcelos, Beatriz M.C. de
Frable, Benjamin W.
Santos, Bernardo F.
Ferraz, Bernardo R.
Rosa, Brunno B.
Sampaio, Brunno H.L.
Bellini, Bruno C.
Clarkson, Bruno
Oliveira, Bruno G. de
Corrêa, Caio C.D.
Martins, Caleb C.
Castro-Guedes, Camila F. de
Souto, Camilla
Bicho, Carla de L.
Cunha, Carlo M.
Barboza, Carlos A. de M.
Lucena, Carlos A.S. de
Barreto, Carlos
Santana, Carlos D.C.M. de
Agne, Carlos E.Q.
Mielke, Carlos G.C.
Caetano, Carlos H.S.
Flechtmann, Carlos H.W.
Lamas, Carlos J.E.
Rocha, Carlos
Mascarenhas, Carolina S.
Margaría, Cecilia B.
Waichert, Cecilia
Digiani, Celina
Haddad, Célio F.B.
Azevedo, Celso O.
Benetti, Cesar J.
Santos, Charles M.D. dos
Bartlett, Charles R.
Bonvicino, Cibele
Ribeiro-Costa, Cibele S.
Santos, Cinthya S.G.
Justino, Cíntia E.L.
Canedo, Clarissa
Bonecker, Claudia C.
Santos, Cláudia P.
Carvalho, Claudio J.B. de
Gonçalves, Clayton C.
Galvão, Cleber
Costa, Cleide
Oliveira, Cléo D.C. de
Schwertner, Cristiano F.
Andrade, Cristiano L.
Pereira, Cristiano M.
Sampaio, Cristiano
Dias, Cristina de O.
Lucena, Daercio A. de A.
Manfio, Daiara
Amorim, Dalton de S.
Queiroz, Dalva L. de
Queiroz, Dalva L. de
Colpani, Daniara
Abbate, Daniel
Aquino, Daniel A.
Burckhardt, Daniel
Cavallari, Daniel C.
Prado, Daniel de C. Schelesky
Praciano, Daniel L.
Basílio, Daniel S.
Bená, Daniela de C.
Toledo, Daniela G.P. de
Takiya, Daniela M.
Fernandes, Daniell R.R.
Ament, Danilo C.
Cordeiro, Danilo P.
Silva, Darliane E.
Pollock, Darren A.
Muniz, David B.
Gibson, David I.
Nogueira, David S.
Marques, Dayse W.A.
Lucatelli, Débora
Garcia, Deivys M.A.
Baêta, Délio
Ferreira, Denise N.M.
Rueda-Ramírez, Diana
Fachin, Diego A.
Souza, Diego de S.
Rodrigues, Diego F.
Pádua, Diego G. de
Barbosa, Diego N.
Dolibaina, Diego R.
Amaral, Diogo C.
Chandler, Donald S.
Maccagnan, Douglas H.B.
Caron, Edilson
Carvalho, Edrielly
Adriano, Edson A.
Abreu Júnior, Edson F. de
Pereira, Edson H.L.
Viegas, Eduarda F.G.
Carneiro, Eduardo
Colley, Eduardo
Eizirik, Eduardo
Santos, Eduardo F. dos
Shimbori, Eduardo M.
Suárez-Morales, Eduardo
Arruda, Eliane P. de
Chiquito, Elisandra A.
Lima, Élison F.B.
Castro, Elizeu B. de
Orlandin, Elton
Nascimento, Elynton A. do
Razzolini, Emanuel
Gama, Emanuel R.R.
Araujo, Enilma M. de
Nishiyama, Eric Y.
Spiessberger, Erich L.
Santos, Érika C.L. dos
Contreras, Eugenia F.
Galati, Eunice A.B.
Oliveira Junior, Evaldo C. de
Gallardo, Fabiana
Hernandes, Fabio A.
Lansac-Tôha, Fábio A.
Pitombo, Fabio B.
Dario, Fabio Di
Santos, Fábio L. dos
Mauro, Fabio
Nascimento, Fabio O. do
Olmos, Fabio
Amaral, Fabio R.
Schunck, Fabio
Godoi, Fábio S. P. de
Machado, Fabrizio M.
Barbo, Fausto E.
Agrain, Federico A.
Ribeiro, Felipe B.
Moreira, Felipe F.F.
Barbosa, Felipe F.
Silva, Fenanda S.
Cavalcanti, Fernanda F.
Straube, Fernando C.
Carbayo, Fernando
Carvalho Filho, Fernando
Zanella, Fernando C.V.
Jacinavicius, Fernando de C.
Farache, Fernando H.A.
Leivas, Fernando
Dias, Fernando M.S.
Mantellato, Fernando
Vaz-de-Mello, Fernando Z.
Gudin, Filipe M.
Albuquerque, Flávio
Molina, Flavio B.
Passos, Flávio D.
Shockley, Floyd W.
Pinheiro, Francielly F.
Mello, Francisco de A.G. de
Nascimento, Francisco E. de L.
Franco, Francisco L.
Oliveira, Francisco L. de
Melo, Francisco T. de V.
Quijano, Freddy R.B.
Salles, Frederico F.
Biffi, Gabriel
Queiroz, Gabriel C.
Bizarro, Gabriel L.
Hrycyna, Gabriela
Leviski, Gabriela
Powell, Gareth S.
Santos, Geane B. dos
Morse, Geoffrey E.
Brown, George
Mattox, George M.T.
Zimbrão, Geraldo
Carvalho, Gervásio S.
Miranda, Gil F.G.
Moraes, Gilberto J. de
Lourido, Gilcélia M.
Neves, Gilmar P.
Moreira, Gilson R.P.
Montingelli, Giovanna G.
Maurício, Giovanni N.
Marconato, Gláucia
Lopez, Guilherme E.L.
Silva, Guilherme L. da
Muricy, Guilherme
Brito, Guilherme R.R.
Garbino, Guilherme S.T.
Flores, Gustavo E.
Graciolli, Gustavo
Libardi, Gustavo S.
Proctor, Heather C.
Gil-Santana, Helcio R.
Varella, Henrique R.
Escalona, Hermes E.
Schmitz, Hermes J.
Rodrigues, Higor D.D.
Galvão Filho, Hilton de C.
Quintino, Hingrid Y.S.
Pinto, Hudson A.
Rainho, Hugo L.
Miyahira, Igor C.
Gonçalves, Igor de S.
Martins, Inês X.
Cardoso, Irene A.
Oliveira, Ismael B. de
Franz, Ismael
Fernandes, Itanna O.
Golfetti, Ivan F.
S. Campos-Filho, Ivanklin
Oliveira, Ivo de S.
Delabie, Jacques H.C.
Oliveira, Jader de
Prando, Jadila S.
Patton, James L.
Bitencourt, Jamille de A.
Silva, Janaina M.
Santos, Jandir C.
Arruda, Janine O.
Valderrama, Jefferson S.
Dalapicolla, Jeronymo
Oliveira, Jéssica P.
Hájek, Jiri
Morselli, João P.
Narita, João P.
Martin, João P.I.
Grazia, Jocélia
McHugh, Joe
Cherem, Jorge J.
Farias Júnior, José A.S.
Fernandes, Jose A.M.
Pacheco, José F.
Birindelli, José L.O.
Rezende, José M.
Avendaño, Jose M.
Duarte, José M. Barbanti
Ribeiro, José R. Inácio
Mermudes, José R.M.
Pujol-Luz, José R.
Santos, Josenilson R. dos
Câmara, Josenir T.
Teixeira, Joyce A.
Prado, Joyce R. do
Botero, Juan P.
Almeida, Julia C.
Kohler, Julia
Gonçalves, Julia P.
Beneti, Julia S.
Donahue, Julian P.
Alvim, Juliana
Almeida, Juliana C.
Segadilha, Juliana L.
Wingert, Juliana M.
Barbosa, Julianna F.
Ferrer, Juliano
Santos, Juliano F. dos
Kuabara, Kamila M.D.
Nascimento, Karine B.
Schoeninger, Karine
Campião, Karla M.
Soares, Karla
Zilch, Kássia
Barão, Kim R.
Teixeira, Larissa
Sousa, Laura D. do N.M. de
Dumas, Leandro L.
Vieira, Leandro M.
Azevedo, Leonardo H.G.
Carvalho, Leonardo S.
Souza, Leonardo S. de
Rocha, Leonardo S.G.
Bernardi, Leopoldo F.O.
Vieira, Letícia M.
Johann, Liana
Salvatierra, Lidianne
Oliveira, Livia de M.
Loureiro, Lourdes M.A. El-moor
Barreto, Luana B.
Barros, Luana M.
Lecci, Lucas
Camargos, Lucas M. de
Lima, Lucas R.C.
Almeida, Lucia M.
Martins, Luciana R.
Marinoni, Luciane
Moura, Luciano de A.
Lima, Luciano
Naka, Luciano N.
Miranda, Lucília S.
Salik, Lucy M.
Bezerra, Luis E.A.
Silveira, Luis F.
Campos, Luiz A.
Castro, Luiz A.S. de
Pinho, Luiz C.
Silveira, Luiz F.L.
Iniesta, Luiz F.M.
Tencatt, Luiz F.C.
Simone, Luiz R.L.
Malabarba, Luiz R.
Cruz, Luiza S. da
Sekerka, Lukas
Barros, Lurdiana D.
Santos, Luziany Q.
Skoracki, Maciej
Correia, Maira A.
Uchoa, Manoel A.
Andrade, Manuella F.G.
Hermes, Marcel G.
Miranda, Marcel S.
Araújo, Marcel S. de
Monné, Marcela L.
Labruna, Marcelo B.
Santis, Marcelo D. de
Duarte, Marcelo
Knoff, Marcelo
Nogueira, Marcelo
Britto, Marcelo R. de
Melo, Marcelo R.S. de
Carvalho, Marcelo R. de
Tavares, Marcelo T.
Kitahara, Marcelo V.
Justo, Marcia C.N.
Botelho, Marcia J.C.
Couri, Márcia S.
Borges-Martins, Márcio
Felix, Márcio
Oliveira, Marcio L. de
Bologna, Marco A.
Gottschalk, Marco S.
Tavares, Marcos D.S.
Lhano, Marcos G.
Bevilaqua, Marcus
Santos, Marcus T.T.
Domingues, Marcus V.
Sallum, Maria A.M.
Digiani, María C.
Santarém, Maria C.A.
Nascimento, Maria C. do
Becerril, María de los A.M.
Santos, Maria E.A. dos
Passos, Maria I. da S. dos
Felippe-Bauer, Maria L.
Cherman, Mariana A.
Terossi, Mariana
Bartz, Marie L.C.
Barbosa, Marina F. de C.
Loeb, Marina V.
Cohn-Haft, Mario
Cupello, Mario
Martins, Marlúcia B.
Christofersen, Martin L.
Bento, Matheus
Rocha, Matheus dos S.
Martins, Maurício L.
Segura, Melissa O.
Cardenas, Melissa Q.
Duarte, Mércia E.
Ivie, Michael A.
Mincarone, Michael M.
Borges, Michela
Monné, Miguel A.
Casagrande, Mirna M.
Fernandez, Monica A.
Piovesan, Mônica
Menezes, Naércio A.
Benaim, Natalia P.
Reategui, Natália S.
Pedro, Natan C.
Pecly, Nathalia H.
Ferreira Júnior, Nelson
Silva Júnior, Nelson J. da
Perioto, Nelson W.
Hamada, Neusa
Degallier, Nicolas
Chao, Ning L.
Ferla, Noeli J.
Mielke, Olaf H.H.
Evangelista, Olivia
Shibatta, Oscar A.
Oliveira, Otto M.P.
Albornoz, Pablo C.L.
Dellapé, Pablo M.
Gonçalves, Pablo R.
Shimabukuro, Paloma H.F.
Grossi, Paschoal
Rodrigues, Patrícia E. da S.
Lima, Patricia O.V.
Velazco, Paul
Santos, Paula B. dos
Araújo, Paula B.
Silva, Paula K.R.
Riccardi, Paula R.
Garcia, Paulo C. de A.
Passos, Paulo G.H.
Corgosinho, Paulo H.C.
Lucinda, Paulo
Costa, Paulo M.S.
Alves, Paulo P.
Roth, Paulo R. de O.
Coelho, Paulo R.S.
Duarte, Paulo R.M.
Carvalho, Pedro F. de
Gnaspini, Pedro
Souza-Dias, Pedro G.B.
Linardi, Pedro M.
Bartholomay, Pedro R.
Demite, Peterson R.
Bulirsch, Petr
Boll, Piter K.
Pereira, Rachel M.M.
Silva, Rafael A.P.F.
Moura, Rafael B. de
Boldrini, Rafael
Silva, Rafaela A. da
Falaschi, Rafaela L.
Cordeiro, Ralf T.S.
Mello, Ramon J.C.L.
Singer, Randal A.
Querino, Ranyse B.
Heleodoro, Raphael A.
Castilho, Raphael de C.
Constantino, Reginaldo
Guedes, Reinaldo C.
Carrenho, Renan
Gomes, Renata S.
Gregorin, Renato
Machado, Renato J.P.
Bérnils, Renato S.
Capellari, Renato S.
Silva, Ricardo B.
Kawada, Ricardo
Dias, Ricardo M.
Siewert, Ricardo
Brugnera, Ricaro
Leschen, Richard A.B.
Constantin, Robert
Robbins, Robert
Pinto, Roberta R.
Reis, Roberto E. dos
Ramos, Robson T. da C.
Cavichioli, Rodney R.
Barros, Rodolfo C. de
Caires, Rodrigo A.
Salvador, Rodrigo B.
Marques, Rodrigo C.
Araújo, Rodrigo C.
Araujo, Rodrigo de O.
Dios, Rodrigo de V.P.
Johnsson, Rodrigo
Feitosa, Rodrigo M.
Hutchings, Roger W.
Lara, Rogéria I.R.
Rossi, Rogério V.
Gerstmeier, Roland
Ochoa, Ronald
Hutchings, Rosa S.G.
Ale-Rocha, Rosaly
Rocha, Rosana M. da
Tidon, Rosana
Brito, Rosangela
Pellens, Roseli
Santos, Sabrina R. dos
Santos, Sandra D. dos
Paiva, Sandra V.
Santos, Sandro
Oliveira, Sarah S. de
Costa, Sávio C.
Gardner, Scott L.
Leal, Sebastián A. Muñoz
Aloquio, Sergio
Bonecker, Sergio L.C.
Bueno, Sergio L. de S.
Almeida, Sérgio M. de
Stampar, Sérgio N.
Andena, Sérgio R.
Posso, Sergio R.
Lima, Sheila P.
Gadelha, Sian de S.
Thiengo, Silvana C.
Cohen, Simone C.
Brandão, Simone N.
Rosa, Simone P.
Ribeiro, Síria L.B.
Letana, Sócrates D.
Santos, Sonia B. dos
Andrade, Sonia C.S.
Dávila, Stephane
Vaz, Stéphanie
Peck, Stewart B.
Christo, Susete W.
Cunha, Suzan B.Z.
Gomes, Suzete R.
Duarte, Tácio
Madeira-Ott, Taís
Marques, Taísa
Roell, Talita
Lima, Tarcilla C. de
Sepulveda, Tatiana A.
Maria, Tatiana F.
Ruschel, Tatiana P.
Rodrigues, Thaiana
Marinho, Thais A.
Almeida, Thaís M. de
Miranda, Thaís P.
Freitas, Thales R.O.
Pereira, Thalles P.L.
Zacca, Thamara
Pacheco, Thaynara L.
Martins, Thiago F.
Alvarenga, Thiago M.
Carvalho, Thiago R. de
Polizei, Thiago T.S.
McElrath, Thomas C.
Henry, Thomas
Pikart, Tiago G.
Porto, Tiago J.
Krolow, Tiago K.
Carvalho, Tiago P.
Lotufo, Tito M. da C.
Caramaschi, Ulisses
Pinheiro, Ulisses dos S.
Pardiñas, Ulyses F.J.
Maia, Valéria C.
Tavares, Valeria
Costa, Valmir A.
Amaral, Vanessa S. do
Silva, Vera C.
Wolff, Vera R. dos S.
Slobodian, Verônica
Silva, Vinícius B. da
Espíndola, Vinicius C.
Costa-Silva, Vinicius da
Bertaco, Vinicius de A.
Padula, Vinícius
Ferreira, Vinicius S.
Silva, Vitor C.P. da
Piacentini, Vítor de Q.
Sandoval-Gómez, Vivian E.
Trevine, Vivian
Sousa, Viviane R.
Sant’Anna, Vivianne B. de
Mathis, Wayne N.
Souza, Wesley de O.
Colombo, Wesley D.
Tomaszewska, Wioletta
Wosiacki, Wolmar B.
Ovando, Ximena M.C.
Leite, Yuri L.R.








ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others.
2.
Lipid Profile of Human Milk in Different Lactation Stages Submitted to Pasteurization, Lyophilization and Spray-Drying Processes Pasteurization SprayDrying Spray Drying
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Neia, Vanessa J. C.
; Santos, Patrícia D. S.
; Tavares, Christyna B. G.
; Paula, Meliana G.
; Costa, Silvio C.
; Zacarias, Joana M. V.
; Alencar, Josiane B.
; Silveira, Roberta
Santos, Oscar O.
Visentainer, Jeane E. L.
Visentainer, Jesuí V.







Journal of the Brazilian Chemical Society
- Métricas do periódico
The objective of this study was to evaluate the effect of pasteurization, lyophilization and spray-drying on the lipid profile of donated human milk (DHM) at different lactation stages. Nine frozen samples of colostrum, transitional and raw mature human milk (HM) were collected from the Human Milk Bank (HMB) of the Hospital Universitário de Maringá (HUM). Samples were thawed and pooled according to lactation stage. Thereafter, it was submitted to pasteurization, lyophilization and spray-drying processes and later the fatty acid (FA) composition and the lipid profile analyses were performed. Gas chromatograph (GC) with flame ionization detector (FID) and electrospray ionization (ESI) mass spectrometer (MS) were used, respectively. In the FA composition analysis, it was observed that the same classes of saturated FA (SFA), monounsaturated FA (MUFA) and polyunsaturated FA (PUFA) were preserved throughout the process and in all lactation stages. The lipid profiles were also preserved after processing. Therefore, the lyophilization and spray-drying processes are promising techniques to preserve the DHM in the HMB, once the components evaluated were preserved, and both techniques facilitate the transport and storage, as the techniques reduce the sample volume. pasteurization spraydrying spray drying (DHM stages colostrum HM (HM HMB (HMB HUM. HUM . (HUM) stage Thereafter (FA performed GC (GC FID (FID ESI (ESI MS (MS used respectively analysis SFA, SFA , (SFA) MUFA (MUFA PUFA (PUFA processing Therefore storage volume (HUM (SFA
3.
Skin-to-skin contact and deaths in newborns weighing up to 1800 grams: a cohort study
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Goudard, Marivanda J.F.
; Lamy, Zeni C.
; Marba, Sérgio T.M.
; Cavalcante, Milady C.V.
; Santos, Alcione M. dos
; Azevedo, Vivian M.G.de O.
; Costa, Roberta
; Guimarães, Carolina N.M.
; Lamy-Filho, Fernando
.









Abstract Objective: To evaluate the association between dose of skin-to-skin contact (SSC) per day and initiation time with the occurrence of deaths in newborns with weight up to 1800g. Method: Multicentric cohort in five Brazilian neonatal units, including newborns with a birth weight of ≤1800g. The time of SSC was registered in individual file, by the team or family during the hospitalization. Maternal and newborn data were obtained through questionnaires applied to mothers and in medical records. Classification Tree was used for data analysis. Results: The performance of the first SSC after 206h was significantly associated with death (p = 0.02). Although there was no association between SSC/day and death (p = 0.09), the number of deaths among those who performed more than 146.9 minutes/day was lower (3;1.5%) than among those who performed this practice for a shorter time (13;6.4%), a fact considered of great clinical importance. Early and late infections present statistically significant associations with the outcome. The chance of death was equal to zero when there was no early infection in the group with the longest duration of SSC. This probability was also equal to zero in the absence of late sepsis for the group with less than 146.9 minutes/day of SSC. Conclusions: The first SSC before 206 hours of life is recommended in order to observe a reduction in the risk of neonatal death. Staying in SSC for more than 146.9 min/day seems to be clinically beneficial for these neonates mostly when it was associated with the absence of infection.
4.
Whey Isolation from Rejected Human Milk and Its Lipid Content Characterization by GC-FID and ESI-MS
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Alves, Eloize S.
; Castro, Matheus C.
; Saqueti, Bruno H. F.
; Manin, Luciana P.
; Silveira, Roberta da
; Souza, Patrícia M.
Santos, Oscar O.
Visentainer, Jesuí V.





Journal of the Brazilian Chemical Society
- Métricas do periódico
The present work intends to obtain and characterize a milk by-product: the human whey (HW), in order to avoid the excessive disposal of human milk (HM). The characterization was performed through analyzes of the chemical composition and fatty acids (FAs) and the triacylglycerol (TAG) lipid profile. The results indicated that the chemical composition altered significantly after the HW obtained, except for the percentage of ash and carbohydrates. About the FA composition, it was observed that strictly essential FA, essential FA and other FAs found in HM remained present in HW. For the nutritional lipids quality, the atherogenicity and the thrombogenicity indices presented desirable values, and the TAGs lipid profile revealed a variation in the analyzed samples. Therefore, it can be concluded that the characterized HW has potential for application in isolated form or to be used as an ingredient in other foods.
https://doi.org/10.21577/0103-5053.20210092
32 downloads
5.
Hydrazones derived from natural aldehydes: in vitro cytotoxic evaluation and in silico pharmacokinetic predictions
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dos Anjos Santos, Victória Laysna
de Assis Gonsalves, Arlan
Souza Silva, Maria Franciele
Evangelista de Oliveira, Fátima de Cássia
Pinheiro da Costa, Marcília
Pessoa, Claudia O.
Melo Araújo, Cleônia Roberta
Revista Colombiana de Ciencias Químico - Farmacéuticas
- Métricas do periódico
RESUMEN Introducción: Investigaciones recientes han informado del potencial citotóxico de las hidrazonas contra varias líneas de células cancerosas. Objetivo: Evaluar la actividad anticancerígena in vitro y el perfil farmacocinético de seis compuestos hidrazónicos sintetizados, identificados como vainillina 1-ftalazinilhidrazona (VAN-1); vainillina 2,4-dinitrofenilhidrazona (VAN-2); fenilhidrazona cinamal-dehído (CIN-1); cinamaldehído de isonicotinoil hidrazona (CIN-2); cinamalde-hído 1-ftalazinilhidrazona (CIN-3); y 2,4-dinitrofenilhidrazona cinamaldehído (CIN-4). Se evaluó la actividad citotóxica frente a cuatro líneas celulares cancerosas. Metodología: Los parámetros farmacocinéticos de absorción, distribución, metabolismo, excreción y toxicidad (ADME/T) de las hidrazonas se evaluaron mediante el programa PreADMET. Resultados: Las hidrazonas derivadas del cinamaldehído (CIN-1 y CIN-2) mostraron una alta actividad citotóxica contra las líneas celulares leucémicas (HL-60) y glioblastomas (SF-295). El perfil farmacocinético de las hidrazonas mostró que, en general, las hidrazonas mostraban características satisfactorias de ADME/T. Además, se observó que CIN-2 presentó el perfil in silico más prometedor, presentando alta absorción intestinal, perfil de distribución deseable relacionado con la unión a proteínas plasmáticas, excreción renal adecuada y baja toxicidad. El perfil ADME/T del compuesto CIN-1 destacó su potencial como agente antineoplásico prometedor con acción sobre el SNC, más específicamente contra los glioblastomas.
SUMMARY Introduction: Recent research has reported the cytotoxic potential of hydrazones against various strains of cancer cells. Aim: To evaluate the anticancer activity in vitro and the pharmacokinetic profile of six synthesized hydrazonic compounds, identified as vanillin 1-phthalazinylhydrazone (VAN-1); 2,4-dinitrophenylhydra-zone vanillin (VAN-2); phenylhydrazone cinnamaldehyde (CIN-1); isonicotinoyl hydrazone cinnamaldehyde (CIN-2); cinnamaldehyde 1-phthalazinylhydrazone (CIN-3); and 2,4-dinitrophenylhydrazone cinnamaldehyde (CIN-4). The cytotoxic activity was evaluated against four strains of cancer cells. Methodology: The pharmacokinetic parameters of absorption, distribution, metabolism, excretion, and toxicity (ADME/T) of the hydrazones were evaluated using the PreADMET program. Results: Hydrazones derived from cinnamaldehyde (CIN-1 and CIN-2) showed high cytotoxic activity against leukemic (HL-60) and glioblastomas (SF-295) cell lines. The pharmacokinetic profile of the hydrazones showed that, in general, the hydrazones presented satisfactory characteristics of ADME/T. In addition, it was observed that CIN-2 presented the most promising in silico profile, showing high intestinal absorption, desirable distribution profile related to plasma protein binding, adequate renal excretion, and low toxicity. The ADME/T profile of the CIN-1 compound highlighted its potential as a promising antineoplastic agent with action of the CNS, more specifically against glioblastomas.
RESUMO Introdução: Pesquisas recentes relataram o potencial citotóxico das hidrazonas contra várias linhagens de células cancerígenas. Objetivo: Validara atividade anti-câncer in vitro e o perfil farmacocinético de seis compostos hidrazônicos sintetizados, identificados como vanilina 1-ftalazinil-hidrazona (VAN-1); vanilina 2,4-dinitrofenil-hidrazona (VAN-2); cinnamaldeído de fenil-hidrazona (CIN-1); cinamaldeído isonicotinoil-hidrazona (CIN-2); 1-ftalazinil-hidrazona de cinnamaldeído (CIN-3); e cinamaldeído de 2,4-dinitrofenil-hidrazona (CIN-4). A atividade citotóxica foi avaliada contra quatro linhagens de células cancerígenas. Metodologia: Os parâmetros farmacocinéticos de absorção, distribuição, metabolismo, excreção e toxicidade (ADME/T) das hidrazonas foram avaliados utilizando o programa PreADMET. Resulados: As hidrazonas derivadas do cinnamaldeído (CIN-1 e CIN-2) apresentaram alta atividade citotóxica contra as linhagens celulares leucêmicas (HL-60) e de glioblastomas (SF-295). O perfil farmacocinético das hidrazonas mostrou que, em geral, as hidrazonas apresentaram características satisfatórias de ADME/T. Além disso, observou-se que a CIN-2 apresentou o perfil in silico mais promissor, exibindo alta absorção intestinal, perfil de distribuição desejável relacionado à ligação às proteínas plasmáticas, excreção renal adequada e baixa toxicidade. O perfil ADME/T do composto CIN-1 destacou seu potencial como um agente antineoplásico promissor com ação do SNC, mais especificamente contra glioblastomas.
6.
PhotoMetrix UVC: A New Smartphone-Based Device for Digital Image Colorimetric Analysis Using PLS Regression
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Costa, Adilson B. da
; Helfer, Gilson A.
; Barbosa, Jorge L. V.
; Teixeira, Iberê D.
Santos, Roberta O.
Santos, Ronaldo B. dos
Voss, Mônica
Schlessner, Sandra K.
Barin, Juliano S.



Journal of the Brazilian Chemical Society
- Métricas do periódico
A novel free PhotoMetrix UVC is proposed for both the operation of a universal serial bus video camera (UVC) and multivariate image analysis, allowing a full solution for point-of-use analysis. A UVC was placed in an open-source 3D-printed chamber illuminated by a white light-emitting diode (LED) with controlled intensity of light. The digital images captured were converted into red, green, and blue (RGB) histograms, and regression models were used within the app. As a proof-of-concept, four adulterants in raw milk samples were determined. The coefficient of determination (R2Cal) for all models was higher than 0.99, and no significant differences (p < 0.05) between the measured and predicted values were identified. The root mean square error of calibration (RMSEC) and root mean square error of cross validation (RMSECV) were satisfactory, with values less than 0.1 and 0.7 g L-1, respectively. The recoveries ranged from 90 to 120% in spiked milk samples, and partial least square (PLS) models showed root mean square error of prediction (RMSEP) of 0.28, 0.33, 0.48 and 0.39 g L-1 for chloride, hypochlorite, hydrogen peroxide and starch, respectively. The PhotoMetrix UVC app was feasible for the colorimetric chemical analysis using a smartphone improving the applicability, mobility, and usability.
https://doi.org/10.21577/0103-5053.20200199
365 downloads
7.
Green Extraction Optimization of Bioactive Compounds from Rosemary (Rosmarinus officinalis L.) Using Response Surface Methodology
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Lopes, Tiago A. M.
; Godoy, Antonio C.
; Sinosaki, Nayane B. M.
Santos, Patrícia D. S.
Chiavelli, Lucas U. R.
Silveira, Roberta
Figueiredo, Bruno H.
Visentainer, Jesuí V.
Santos, Oscar O.


Journal of the Brazilian Chemical Society
- Métricas do periódico
Rosemary (Rosmarinus officinalis L.) is known for its bioactive compounds with high antioxidant potential and pharmacological properties, such as antibacterial, antidepressant and anti-inflammatory. These properties are attributed to the presence of (poly)phenolic compounds. In the literature there is no extraction of these compounds with a totally non-toxic solvent, capable of applying the extract directly to the food. This work aimed to optimize the extraction conditions of bioactive compounds from the rosemary leaves using water as solvent. According to the analysis of variance parameters, a cubic model of high order of significance was obtained with adjusted R2 (R squared) > 0.947. The optimal conditions of the antioxidant potential for conventional and ultrasonic agitation were: temperature 70 °C, 30 min of extraction and the solvent/solute ratio of 25, resulting in an antioxidant potential of 6861 and 7126 µmol L−1 Trolox, respectively. The electrospray ionization mass spectrometry (ESI(−)-MS/MS) analysis showed the presence of antioxidant compounds such as: carnosol (m/z 329.20), rosmanol (m/z 345.03) and rosmarinic (m/z359.14) acids. The response surface methodology evaluated the influence of the main parameters of the bioactive compounds extraction from rosemary using only water as solvent, in addition to the use of less time and sample mass, resulting in lower cost.
https://doi.org/10.21577/0103-5053.20200142
371 downloads
8.
Evaluation of Dog Food Authenticity through Lipid Profile Using GC-FID and ESI-MS
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Silveira, Roberta da
; Santos, Patrícia D. S. dos
; Pizzo, Jessica S.
; Mattos, Nayane
; Castro, Matheus C. de
Santos, Oscar O.
Visentainer, Jesuí V.




Journal of the Brazilian Chemical Society
- Métricas do periódico
Considering the increasing number of low cost vegetable oil product adulterations, it is necessary to assess the authenticity of the lipid sources used in the dog food manufacture, consequently different brands and classifications of feed were analyzed to verify the product authenticity by gas chromatography (GC) with flame ionization detector (FID) and by electrospray ionization mass spectrometry (ESI-MS). Fatty acid (FA) composition analysis was performed to compare the FAs amount in samples with the respective guarantee levels stated by the manufacturers on its product label. Gas chromatography with flame ionization detector (GC-FID) results for omega-3 and 6 percentages are within the limits presented by each manufacturer, while the timnodonic + cervonic acids amount information presented on feed sample labels is not in accordance with GC-FID results. Consequently, the samples were analyzed by ESI-MS, the results exposed the chicken oil presence in the feed and eicosapentaenoic (EPA) + docosahexaenoic (DHA) acids absence, since no lipid profiles similar to fish oils or triacylglycerols (TAGs) containing these two FAs were found. Thus, the information presented on labels are in disagreement with the results obtained both by GC-FID FA composition analysis and by ESI-MS lipid profile analysis. Principal component analysis (PCA) confirmed that the main contribution was from FAs found by GC-FID.
https://doi.org/10.21577/0103-5053.20200126
297 downloads
9.
Structural Study of Phenolic Acids by Triple Quadrupole Mass Spectrometry with Electrospray Ionization in Negative Mode and H/D Isotopic Exchange
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Sinosaki, Nayane B. M.
; Tonin, Angélica P. P.
; Ribeiro, Marcos A. S.
Poliseli, Camila B.
Roberto, Sharise B.
Silveira, Roberta da
Visentainer, Jesuí V.
Santos, Oscar O.
Meurer, Eduardo C.


Journal of the Brazilian Chemical Society
- Métricas do periódico
This work reports the theoretical and experimental study of fragmentation reactions in the gas phase of five phenolic acids using triple quadrupole mass spectrometry by electrospray ionization in negative ionic mode, as well as the isotope exchange experiments. MS/MS spectra were analyzed to suggest the fragmentation mechanisms, while theoretical calculations at the theory level B3LYP/6-311+G** were performed to expose the proposed mechanisms viability for this class of compounds. As expected, compounds with aromatic methoxy substitution presented •CH3 radical elimination as the principal fragmentation pathway, forming dystonic ions. Compounds without methoxy substituents dissociate with higher energies losing the CO2, CO and H2O. The isotopic marking experiments indicated the exchange of hydrogens by deuterases in the hydroxyl protons, which corroborates with the proposed mechanisms.
https://doi.org/10.21577/0103-5053.20190197
1126 downloads
10.
Comment on the study Coronary Artery Bypass Surgery in Brazil: Analysis of the National Reality Through the Bypass Registry that was presented at the 46th Congress of the Brazilian Society of Cardiovascular Surgery, Nova Lima, BH, Brazil, April 5 and 6, 2019
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Dallan, Luís Alberto O.
; Paez, Rodrigo Pereira
; Hossne Junior, Nelson Américo
Santo, José Amalth do Espírito
Berwanger, Otavio
Santos, Renato Hideo Nakagawa
Kalil, Renato Abdala Karam
Jatene, Fabio B.
Cavalcanti, Alexandre Biasi
Zilli, Alexandre Cabral
Bettiati Jr, Luiz Carlos
Figueira, Fernando Augusto Marinho dos Santos
D’Azevedo, Stephanie Steremberg Pires
Soares, Marcelo José Ferreira
Fernandes, Marcio Pimentel
Ardito, Roberto Vito
Bogdan, Renata Andrea Barberio
Campagnucci, Valquíria Pelisser
Nakasako, Diana
Rodrigues, Clarissa Garcia
Rodrigues Junior, Anilton Bezerra
Cascudo, Marcelo Matos
Atik, Fernando Antibas
Lima, Elson Borges
Nina, Vinicius José da Silva
Heluy, Renato Albuquerque
Azeredo, Lisandro Gonçalves
Henrique Junior, Odilon Silva
Mendonça, José Teles de
Silva, Katharina Kelly de Oliveira Gama
Pandolfo, Marcelo
Lima Júnior, José Dantas de
Faria, Renato Max
Santos, Jonas Gonçalves dos
Coelho, Guilherme Henrique Biachi
Pereira, Sergio Nunes
Senger, Roberta
Buffolo, Enio
Caputi, Guido Marco
Oliveira, Juliana Aparecida Borges de
Gomes, Walter J.


Brazilian Journal of Cardiovascular Surgery
- Métricas do periódico
https://doi.org/10.21470/1678-9741-2019-0606
335 downloads
11.
Fatty Acid Determination in Fermented Milk Samples Employing Direct Esterification and Gas Chromatography
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Piccioli, Angélica F. B.
; Santos, Patrícia D. S.
; Silveira, Roberta da
Bonafé, Elton
Visentainer, Jesuí V.
Santos, Oscar O.


Journal of the Brazilian Chemical Society
- Métricas do periódico
The developed method (DM) allows to perform experiments in shorter time with reduced amount of sample and reagent for fatty acids (FA) determination in fermented milk samples. Design Expert software was employed to discover the greatest sonication time (7 and 20 min) and concentration for alkaline and acidic reactions (0.70 and 1.5 mol L-1). Validation procedures were applied in yogurt samples with diverse fat content, ranging from 1.22 to 2.83%, being in agreement with acceptable values. Besides, the intraday relative standard deviation (RSD) (1.86%) and interday RSD (4.23%) values confirmed the good accuracy of the proposed method for FA derivatization.
https://doi.org/10.21577/0103-5053.20190027
488 downloads
12.
Simultaneous Determination of Four Antibiotics in Raw Milk by UPLC-MS/MS Using Protein Precipitation as Sample Preparation: Development, Validation, and Application in Real Samples
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Magon, Thiago
Silveira, Roberta da
Galuch, Marília B.
Fagan, Eder Paulo
Feitoza, Ana Flavia D.
Palombini, Sylvio V.
Santos, Oscar O.
Visentainer, Jesui V.
Journal of the Brazilian Chemical Society
- Métricas do periódico
In this study, a rapid and simple analytical method was proposed, based on protein precipitation as sample preparation for simultaneous determination of tetracycline, oxytetracycline, penicillin G and ceftiofur in raw milk by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The method was applied to raw milk samples from dairy cows medicated with tetracycline for subclinical mastitis. Samples were collected from the adjacent teat to the teat treated with tetracycline of eight different cows at 24, 48, 72 and 96 h after treatment. The limits of quantification of the proposed method ranged between 1 and 5 ng g-1 and limits of detection ranged between 0.1 and 0.5 ng g-1. The recoveries ranged from 61 to 111% and the linear range was 1 to 2064 ng g-1 for tetracycline and oxytetracycline, and 5 to 2064 ng g-1 for penicillin G and ceftiofur. Approximately 75 and 63% of the treated animals revealed more tetracycline than legally recommended at 72 and 96 h since last treatment, respectively.
https://doi.org/10.21577/0103-5053.20180121
1040 downloads
13.
Fatty Acid Composition and Carotenoids in Raw and Grilled Tilapia GIFT Fillets Supplemented with Lycopene, β-Carotene and Lutein
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Reis, Elaine C.
Galuch, Marília B.
Silveira, Roberta da
Santos, Hevelyse M. C. dos
Sary, Cesar
Magon, Thiago F. S.
Figueiredo, Ingrid L.
Ribeiro, Ricardo P.
Visentainer, Jesuí V.
Santos, Oscar O.
Journal of the Brazilian Chemical Society
- Métricas do periódico
This study evaluated the incorporation of β-carotene, lutein and lycopene into tilapia (genetically improved farmed tilapia, GIFT) fillets supplemented through 0, 20 and 40 days, and their influence on the fatty acid composition and nutritional quality of raw and grilled fillets. A control diet and a diet containing those carotenoids were elaborated with the same lipid source (soybean oil). For carotenoids analysis, the extraction was performed according to the literature, employing acetone and hexane. The relative intensities of m/z ratios of those carotenoids ions were obtained by ultraperformance liquid chromatography-tandem mass spectrometry with chemical ionization at atmospheric pressure (UPLC-APCI MS/MS), and an increase of carotenoid concentration in the supplemented fillets was observed, especially after the grilling process (180 ºC for 15 min). An increase in the concentration of total carotenoids in grilled fillets (75 and 35% higher than raw fillets, for 20 and 40 days of supplementation, respectively, corresponding to 8.32 and 2.42 µg β-carotene g-1 sample) was also observed. Therefore, the carotenoids promoted the stability of lipid oxidation in grilled samples; fact that can be correlated with antioxidant effect of these compounds, it can be also explained by the breakdown of carotenoids physical barrier and the conditions used in the grilling process. Moreover, acceptable n-6/n-3 indices and recommended polyunsaturated fatty acids/saturated fatty acids (PUFA/SFA) values were observed in supplemented fillets. Consequently, lutein, β-carotene and lycopene were considered viable as additive on fish diet, since the supplementation promoted the increase of food quality, even after preparation for consumption.
https://doi.org/10.21577/0103-5053.20180049
563 downloads
14.
Analytical Method Development for Fatty Acid Direct Methylation in Fruits
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Journal of the Brazilian Chemical Society
- Métricas do periódico
Due to the amount of pigments, sugars and fibers found in fruits, the fatty acid quantification process leads to errors, such as its amount underestimation in the matrix. Therefore, the proposed method allows performing experiments in shorter time with reduced amount of sample and reagent. Design Expert software was used to find the validated time for alkaline and acidic reactions, which were six and eight minutes, respectively, and both concentration was 0.5 mol L-1. The validation was made for avocado pulp and different fruits were used for application of the method, such as: apple, mango, coconut and another avocado variety. Accuracy values ranged from 89.05 to 100.21%, being in accordance with acceptable values. Also the relative standard deviation (RSD)intra-day (0.30 to 1.30%) and the RSDinter-day values (0.32 to 1.44%) confirmed that the proposed method for fatty acid derivatization has good precision.
https://doi.org/10.21577/0103-5053.20180036
827 downloads
15.
Quality Assessment of Omega-3 Supplements Available in the Brazilian Market
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Galuch, Marília B.
Carbonera, Fabiana
Magon, Thiago F. S.
Silveira, Roberta da
Santos, Patrícia D. S. dos
Pizzo, Jessica S.
Santos, Oscar O.
Visentainer, Jesuí V.
Journal of the Brazilian Chemical Society
- Métricas do periódico
Omega-3 fish oil supplements are widely consumed as source of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, presenting beneficial effects on human health. This study aimed to evaluate fifteen brands of omega-3 fish oil supplements available in Brazilian market in order to estimate the Brazilian reality regarding those supplements. Twelve fatty acids were quantified by gas chromatography with a flame ionization detector (GC-FID), and lipid profile were obtained via mass spectrometry fingerprinting using direct electrospray ionization mass spectrometry (ESI-MS) to assess the form in which fatty acids are present as well as the possible fraud existence. Among all analyzed samples, thirteen brands were revealed as EPA and DHA sources (90.2-440.3 and 77.8-302.3 mg g-1lipid, respectively) in triacylglycerols (TAG) or ethyl esters (EE) form. However, two brands were discovered with addition of large amounts of soybean oil, leading the final consumer to ingest this low-cost oil believing that they are consuming adequate doses of EPA and DHA.
https://doi.org/10.21577/0103-5053.20170177
2144 downloads
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