Abstract Working with an interprofessional focus is increasingly necessary, in view of the growing complexity of the population’s health needs. This study aims to assess interprofessional collaboration and the teamwork climate in primary health care (PHC) and determine whether there is a relationship between these two variables. The AITCS-II instrument was used to measure interprofessional collaboration, while to diagnose teamwork climate, the ECTE instrument was used, a version adapted to the SUS context of the Teamwork Climate Inventory instrument. These two instruments were applied online together with a questionnaire for the sociodemographic characterization of the 544 participants, who belonged to 97 Family Health Strategy (FHS) teams in a Brazilian municipality. The obtained data were submitted to a multilevel analysis. A positive correlation was observed between interprofessional collaboration and three of the four teamwork climate factors. The better the work climate, the better the interprofessional collaboration in the corresponding team, and this characteristic stands out in relation to other individual analyzed characteristics. necessary populations population s needs PHC (PHC variables AITCSII AITCS II 54 participants 9 FHS (FHS municipality analysis factors team characteristics 5

Resumo Trabalhar com foco interprofissional é cada vez mais necessário, tendo em vista a crescente complexidade das necessidades de saúde da população. Este estudo tem como objetivo avaliar a colaboração interprofissional e o clima de trabalho em equipe na atenção primária à saúde (APS) e verificar possível relação entre estas duas variáveis. Para mensurar a colaboração interprofissional foi utilizado o instrumento AITCS-II, enquanto para o diagnóstico do clima de trabalho em equipe foi utilizado o instrumento ECTE, versão adaptada para o contexto SUS do instrumento Teamwork Climate Inventory. Esses dois instrumentos foram aplicados on-line juntamente com um questionário para caracterização sociodemográfica dos 544 participantes, pertencentes a 97 equipes da Estratégia Saúde da Família (ESF) de um município brasileiro. Os dados obtidos foram submetidos a uma análise multinível. Foi observada uma correlação positiva entre a colaboração interprofissional e três dos quatro fatores do clima de trabalho em equipe. Quanto melhor o clima de trabalho, melhor a colaboração interprofissional na equipe correspondente, e essa característica se destaca em relação às demais características individuais analisadas. necessário população APS (APS variáveis AITCSII, AITCSII AITCS II, II AITCS-II ECTE Inventory online on line 54 participantes 9 ESF (ESF brasileiro multinível correspondente analisadas 5

ABSTRACT Purpose: To evaluate, by quantitative and qualitative methods, the glomerular ultrastructure in Wistar rats fed a cafeteria diet. Methods: Male Wistar rats were divided into two groups at 21 days of age: control (C, n = 10) and cafeteria diet (CAF, n = 8). The animals were followed up until 5 months of age, followed by euthanasia. The blood, kidneys, and fat deposits––epididymal, retroperitoneal, and subcutaneous––were extracted and analyzed. Data were analyzed by Student’s t test, and p < 0.05 was considered statistically significant. Results: The cafeteria diet promoted glucose intolerance, hyperglycemia (p < 0.0001), and deposition of retroperitoneal fat (p < 0.005). Scanning electron microscopy revealed that the length of the foot process was similar in both groups. The quantitative analyses by transmission electron microscopy revealed that the cafeteria diet reduced the thickness of the glomerular basement membrane (p < 0.05). Conclusions: The intake of lipids and simple carbohydrates were found to be associated with alteration in the glomerular ultrastructure. However, more studies are needed to evaluate not only the effects of high-protein and high-fat diets on components of the glomerular filtration barrier, but also renal physiology. Purpose methods Methods 2 age C, C (C 10 CAF, CAF (CAF 8. 8 . 8) euthanasia blood kidneys depositsepididymal deposits epididymal deposits––epididymal subcutaneouswere subcutaneous Students Student s test 005 0 05 0.0 significant Results intolerance 0.0001, 00001 0.0001 , 0001 0.0001) 0.005. 0005 0.005 0.005) 0.05. 0.05) Conclusions However highprotein high protein highfat barrier physiology 1 00 0. 0000 0.000 000 0.00

Generalized anxiety disorder is a highly prevalent mental disorder. Previous data indicate that more than 18 million Brazilians suffer from this condition. Traditionally, generalized anxiety disorder has been considered a mild mental health disorder, despite its links to lower life expectancy, cardiovascular disease, and suicide. The aim of this article is to combine elements of systematic and critical reviews to produce a synthesis of the best evidence about generalized anxiety disorder treatment. Systematic reviews, meta-analyses, and randomized controlled trials were included. The descriptor used in the search was “generalized anxiety disorder,” which resulted in 4,860 articles and seven other studies, of which 59 were selected. Antidepressants and benzodiazepines were indicated, as was pregabalin, and atypical antipsychotics, such as quetiapine, have been studied. Individual cognitive behavior therapy (third wave) has proven effective. There is extensive literature on many effective treatments for generalized anxiety disorder. The present review summarizes the therapeutic possibilities, emphasizing those available in Brazil. Further studies are needed to compare other available medications, assess psychotherapies and new treatments in greater depth, as well as to assess the ideal duration of therapy. Registration number: PROSPERO CRD42021288323. 1 condition Traditionally expectancy disease suicide treatment metaanalyses, metaanalyses meta analyses, analyses meta-analyses included 4860 4 860 4,86 5 selected indicated pregabalin antipsychotics quetiapine studied third wave possibilities Brazil medications depth number CRD42021288323 CRD 486 86 4,8 CRD4202128832 48 8 4, CRD420212883 CRD42021288 CRD4202128 CRD420212 CRD42021 CRD4202 CRD420 CRD42 CRD4

Abstract Working with an interprofessional focus is increasingly necessary, in view of the growing complexity of the population’s health needs. This study aims to assess interprofessional collaboration and the teamwork climate in primary health care (PHC) and determine whether there is a relationship between these two variables. The AITCS-II instrument was used to measure interprofessional collaboration, while to diagnose teamwork climate, the ECTE instrument was used, a version adapted to the SUS context of the Teamwork Climate Inventory instrument. These two instruments were applied online together with a questionnaire for the sociodemographic characterization of the 544 participants, who belonged to 97 Family Health Strategy (FHS) teams in a Brazilian municipality. The obtained data were submitted to a multilevel analysis. A positive correlation was observed between interprofessional collaboration and three of the four teamwork climate factors. The better the work climate, the better the interprofessional collaboration in the corresponding team, and this characteristic stands out in relation to other individual analyzed characteristics.

Resumo Trabalhar com foco interprofissional é cada vez mais necessário, tendo em vista a crescente complexidade das necessidades de saúde da população. Este estudo tem como objetivo avaliar a colaboração interprofissional e o clima de trabalho em equipe na atenção primária à saúde (APS) e verificar possível relação entre estas duas variáveis. Para mensurar a colaboração interprofissional foi utilizado o instrumento AITCS-II, enquanto para o diagnóstico do clima de trabalho em equipe foi utilizado o instrumento ECTE, versão adaptada para o contexto SUS do instrumento Teamwork Climate Inventory. Esses dois instrumentos foram aplicados on-line juntamente com um questionário para caracterização sociodemográfica dos 544 participantes, pertencentes a 97 equipes da Estratégia Saúde da Família (ESF) de um município brasileiro. Os dados obtidos foram submetidos a uma análise multinível. Foi observada uma correlação positiva entre a colaboração interprofissional e três dos quatro fatores do clima de trabalho em equipe. Quanto melhor o clima de trabalho, melhor a colaboração interprofissional na equipe correspondente, e essa característica se destaca em relação às demais características individuais analisadas.

Abstract The Pantanal comprehends a set of heterogeneous and biodiverse landscapes of esteemed environmental, economic and social value. The study of soils is effective to stratify and to understanding the operation of these environments. The purpose of this study was to characterize and compare soils of lake landscapes of the Pantanal: baías (freshwater lakes), salinas (alkaline lakes) and their respective levees (sandy ridges originally with forest vegetation). For this purpose, we carried out field work with sampling, in triplicate, of superficial soil layers in 12 representative areas of low and high Nhecolândia; analytical determinations of 26 soil attributes were performed, totaling 936 response variables and statistical analyses were performed in order to synthesize the data and present the results. In both landscapes, the fine sand fraction predominates in the soil granulometry. The textural class of the soils varied from very sandy, medium sandy and medium sandy for the baías; very sandy for the levees, with particle density related to the presence of quartz; and very sandy, medium sandy, medium clayey and clayey for the salinas. In the chemical attributes and organic matter, the baías stand out for higher potential acidity (H+Al), slightly high organic matter contents and availability of metal ions, especially Fe; in the levees, higher average remaining phosphorus (Prem) are more evident; while the saline lake soils are related to more alkaline pH values, high base saturation and high Sodium Saturation Indices (ISNa). Salinas landscapes presented the highest data variability and have soil attributes that refer to the action of different environmental processes. value environments freshwater lakes, lakes , vegetation. vegetation . vegetation) sampling triplicate 1 Nhecolândia 2 93 results granulometry quartz H+Al, HAl H+Al H Al (H+Al) ions Fe Prem (Prem evident values ISNa. ISNa (ISNa) processes 9 (H+Al (ISNa

Resumo O Pantanal compreende um conjunto de paisagens heterogêneas e biodiversas de estimado valor ambiental, econômico e social. O estudo dos solos é efetivo para estratificar e compreender o funcionamento desses ambientes. Objetivou-se caracterizar e comparar solos de paisagens lacustres do Pantanal Sul-Mato-Grossense: baías (lagos de água doce), salinas (lagos alcalinos) e suas respectivas cordilheiras (cordões arenosos originalmente com vegetação florestal). Para tanto, realizou-se trabalho de campo com amostragens, em triplicata, de camadas superficiais do solo em 12 áreas representativas da baixa e alta Nhecolândia; procedeu-se determinações analíticas de 26 atributos do solo, totalizando 936 variáveis resposta e; análises estatísticas foram realizadas visando a síntese dos dados e apresentação dos resultados. Em ambas as paisagens predomina a fração areia fina na granulometria do solo. A classe textural dos solos variou de muito arenosa, arenosa média e média arenosa para as baías; muito arenosa para as cordilheiras, com Dp relacionada a presença do quartzo; e muito arenosa, arenosa média, média argilosa e argilosa para as salinas. Com relação aos atributos químicos e matéria orgânica, as baías se destacam pela maior acidez potencial (H+Al), teores de MO ligeiramente elevados e disponibilidade de íons metálicos, sobretudo Fe; nas cordilheiras, maiores médias de P-rem são mais evidentes; já os solos de salinas se relacionam a valores de pH mais alcalinos, elevada saturação por bases e altos índices de saturação por Sódio (ISNa). Paisagens de salinas apresentaram a maior variabilidade de dados e possuem atributos do solo que remetem a ação de diferentes processos ambientais. ambiental social ambientes Objetivouse Objetivou SulMatoGrossense Sul Mato Grossense Sul-Mato-Grossense lagos doce, doce , doce) alcalinos cordões florestal. florestal . florestal) tanto realizouse realizou amostragens triplicata 1 Nhecolândia procedeuse procedeu 2 93 resultados quartzo orgânica H+Al, HAl H+Al H Al (H+Al) metálicos Fe Prem P rem evidentes ISNa. ISNa (ISNa) ambientais 9 (H+Al (ISNa

Abstract The objective of this work was to determine the fatty acid profile of artisanal Minas cheese from seven certified regions in the state of Minas Gerais, Brazil. A total of 78 samples were collected in regions where the producers were registered by Instituto Mineiro de Agropecuária. For lipid profile determination, different cheese varieties from the following regions were analyzed: Canastra, Serro, Araxá, Serra do Salitre, Triângulo Mineiro, Campo das Vertentes, and Cerrado. The lipid profile of Serra do Salitre and Araxá cheeses was similar in caproic acid (C6:0), total polyunsaturated fatty acids, and ratio of polyunsaturated and saturated fatty acids. In the other regions, lipid profile differed due to associations with specific fatty acids, such as those of the cheeses from Triângulo Mineiro with C20:5n3, of Cerrado with C20:4n6, of Canastra with C8:0, of Serro with C22:2 and C22:6n3, and of Campo das Vertentes with C17:0, C18:0, and C20:0. The fatty acid profile and nutritional indices related to the lipid profile of artisanal Minas cheeses differ according to the region of origin. The content and profile of omega 3 fatty acids are significant parameters to differentiate artisanal Minas cheeses. Serro artisanal Minas cheese presents superior nutritional indices concerning fatty acid profile than the other studied cheeses. Gerais Brazil 7 Agropecuária determination analyzed C60, C60 C C6 0 , (C6:0) C205n3, C205n3 Cn C20 5n3, 5n3 n C20:5n3 C204n6, C204n6 4n6, 4n6 C20:4n6 C80, C80 C8 0, C8:0 C222 C22 2 C22: C226n3, C226n3 6n3, 6n3 C22:6n3 C170, C170 C17 C17:0 C180, C180 C18 C18:0 C200. C200 0. C20:0 origin (C6:0 C205n C2 5n C20:5n C204n 4n C20:4n C8: C226n 6n C22:6n C1 C17: C18: C20: (C6: (C6 (C

Resumo O objetivo deste trabalho foi determinar o perfil de ácidos graxos em queijos minas artesanais provenientes de sete regiões certificadas no estado de Minas Gerais, Brasil. Um total de 78 amostras foram coletadas em regiões com produtores cadastrados pelo Instituto Mineiro de Agropecuária. Para determinar a composição lipídica, foram analisadas diferentes variedades de queijo das seguintes regiões: Canastra, Serro, Araxá, Serra do Salitre, Triângulo Mineiro, Campo das Vertentes e Cerrado. A composição lipídica dos queijos da Serra do Salitre e de Araxá foi similar em ácido caproico (C6:0), total de ácidos graxos poli-insaturados e razão entre ácidos poli-insaturados e saturados. Nas outras regiões, a composição lipídica diferiu devido a associações com ácidos graxos específicos, como as dos queijos das regiões do Triangulo Mineiro com C20:5n3, do Cerrado com C20:4n6, da Serra da Canastra com C8:0, do Serro com C22:2 e C22:6n3 e de Campo das Vertentes com C17:0, C18:0 e C20:0. O perfil de ácidos graxos e os índices nutricionais relacionados ao perfil lipídico dos queijos minas artesanais diferem de acordo com a região de origem. O teor e o perfil de ácidos graxos ômega 3 são parâmetros importantes para diferenciar os queijos minas artesanais. O queijo minas artesanal do Serro apresenta melhores índices nutricionais quanto ao perfil de ácidos graxos que os demais queijos estudados. Gerais Brasil 7 Agropecuária C60, C60 C C6 0 , (C6:0) poliinsaturados poli insaturados saturados específicos C205n3, C205n3 Cn C20 5n3, 5n3 n C20:5n3 C204n6, C204n6 4n6, 4n6 C20:4n6 C80, C80 C8 0, C8:0 C222 C22 2 C22: C226n3 6n3 C22:6n C170, C170 C17 C17:0 C180 C18 C18: C200. C200 0. C20:0 origem estudados (C6:0 C205n C2 5n C20:5n C204n 4n C20:4n C8: C226n 6n C1 C17: C20: (C6: (C6 (C

Abstract Introduction: Vascular graft infections are a rare and severe complication of open aortic surgery. As such, strategies to improve diagnosis and management are paramount. Nevertheless, there is little evidence regarding the factors associated with a higher susceptibility of infection. Moreover, there is little consensus on the best diagnostic workup and most adequate approaches to control and mitigate this surgical complication. We aim to summarize the latest evidence on aortic graft infection through a narrative review. Methods: We conducted a literature search in a medical database (PubMed) and included studies on vascular graft infection and aortic repair. Results: So far, patient-related comorbidities (such as diabetes, smoking, advanced age and chronic kidney disease), as well as procedure-related factors (location and type of graft, postoperative hyperglycemia, bacteremia, emergency setting), have been reported. Unlike extracavitary infections, aortic graft infections may present with subtle or no clinical manifestations. Aortoenteric fistulas are the exception to the rule, carrying a significantly higher mortality rate. All patients should be thoroughly tested with full blood counts, blood cultures and extensive imaging studies. Adequate antibiotic therapy is one of the pillars of treatment and should not be delayed. The selection of the antimicrobial regimen should be personalized and made in a multidisciplinary team. Surgical strategies are of paramount importance in controlling infection. These can be divided into graft-sparing techniques and graft explantation. The choice of the appropriate approach depends on the surgeon’s experience, the extent of disease and the patient’s general status and comorbidities. Conclusion: Aortic graft infections are a rare and severe complication of vascular surgery. A swift diagnosis and management are paramount. Despite significant efforts on how to treat the infection with more adequate antibiotic and surgical therapies, effective preventive measures and a clear definition of predisposing factors remain the main areas for future studies.

Resumo Objetivou-se identificar determinantes sociais em saúde associados à vivência da fome entre adolescentes escolares brasileiros. Foi realizado um estudo transversal com microdados de 16.526 adolescentes da Pesquisa Nacional de Saúde do Escolar 2015. A vivência da fome foi estimada considerando a frequência com que o adolescente havia ficado com fome por não ter comida suficiente em casa no mês anterior à pesquisa. Para a análise dos determinantes sociais em saúde foi realizada Regressão de Poisson com variância robusta. Verificou-se, no Brasil, que a vivência da fome foi de 22,8% (IC95%: 21,9-23,7) entre adolescentes. Essa associou-se diretamente ao sexo masculino (RP=1,12; IC95%: 1,07-1,16), a não ter excesso de peso (RP=1,08; IC95%: 1,04-1,13), ao consumo irregular de feijão (RP=1,20; IC95%: 1,13-1,26), de legumes e verduras (RP=1,16; IC95%: 1,09-1,22) e de frutas (RP=1,19; IC95%: 1,13-1,24), à insatisfação corporal (RP=1,26; IC95%: 1,18-1,35) e ao consumo irregular de almoço ou jantar com os responsáveis (RP=1,41; IC95%: 1,32-1,52); e inversamente ao gradiente de escolaridade materna, e às macrorregiões do complexo Centro-Sul. Os resultados indicam a coexistência da fome, comportamentos alimentares de risco nutricional, insatisfação corporal e condições de iniquidade social entre adolescentes brasileiros.

Abstract The aim of this study was to identify social determinants of health associated with the experience of hunger among school-age adolescents in Brazil. We conducted a cross-sectional study with a sample of 16,526 adolescents using data from the 2015 National School-based Student Health Survey. Experience of hunger was determined based on the answer to the question “In the last 30 days, how often have you been hungry because there wasn’t enough food at home?” The social determinants of health were analyzed using Poisson regression with robust variance. The prevalence of the experience of hunger was 22.8% (95%CI: 21.9-23.7). The experience of hunger was directly associated with being male (PR = 1.12; 95%CI: 1.07-1.16); not being overweight (PR = 1.08; 95%CI: 1.04-1.13 ); irregular consumption of beans (PR = 1.20; 95%CI: 1.13-1.26), vegetables (PR=1.16; 95%CI: 1.09-1.22) and fruit (PR = 1.19; 95%CI: 1.13-1.24); body dissatisfaction (PR = 1.26; 95%CI: 1.18-1.35); and not regularly eating lunch or dinner with parents or guardians (PR = 1.41; 95%CI: 1.32-1.52). An inverse association was found between the experience of hunger and maternal education level and living in the Mid-West, Southeast and South. The findings show that the experience of hunger among Brazilian adolescents coexists with risky eating behaviors, body dissatisfaction, and social inequality.

Resumo Objetivou-se identificar determinantes sociais em saúde associados à vivência da fome entre adolescentes escolares brasileiros. Foi realizado um estudo transversal com microdados de 16.526 adolescentes da Pesquisa Nacional de Saúde do Escolar 2015. A vivência da fome foi estimada considerando a frequência com que o adolescente havia ficado com fome por não ter comida suficiente em casa no mês anterior à pesquisa. Para a análise dos determinantes sociais em saúde foi realizada Regressão de Poisson com variância robusta. Verificou-se, no Brasil, que a vivência da fome foi de 22,8% (IC95%: 21,9-23,7) entre adolescentes. Essa associou-se diretamente ao sexo masculino (RP=1,12; IC95%: 1,07-1,16), a não ter excesso de peso (RP=1,08; IC95%: 1,04-1,13), ao consumo irregular de feijão (RP=1,20; IC95%: 1,13-1,26), de legumes e verduras (RP=1,16; IC95%: 1,09-1,22) e de frutas (RP=1,19; IC95%: 1,13-1,24), à insatisfação corporal (RP=1,26; IC95%: 1,18-1,35) e ao consumo irregular de almoço ou jantar com os responsáveis (RP=1,41; IC95%: 1,32-1,52); e inversamente ao gradiente de escolaridade materna, e às macrorregiões do complexo Centro-Sul. Os resultados indicam a coexistência da fome, comportamentos alimentares de risco nutricional, insatisfação corporal e condições de iniquidade social entre adolescentes brasileiros.

Abstract The aim of this study was to identify social determinants of health associated with the experience of hunger among school-age adolescents in Brazil. We conducted a cross-sectional study with a sample of 16,526 adolescents using data from the 2015 National School-based Student Health Survey. Experience of hunger was determined based on the answer to the question “In the last 30 days, how often have you been hungry because there wasn’t enough food at home?” The social determinants of health were analyzed using Poisson regression with robust variance. The prevalence of the experience of hunger was 22.8% (95%CI: 21.9-23.7). The experience of hunger was directly associated with being male (PR = 1.12; 95%CI: 1.07-1.16); not being overweight (PR = 1.08; 95%CI: 1.04-1.13 ); irregular consumption of beans (PR = 1.20; 95%CI: 1.13-1.26), vegetables (PR=1.16; 95%CI: 1.09-1.22) and fruit (PR = 1.19; 95%CI: 1.13-1.24); body dissatisfaction (PR = 1.26; 95%CI: 1.18-1.35); and not regularly eating lunch or dinner with parents or guardians (PR = 1.41; 95%CI: 1.32-1.52). An inverse association was found between the experience of hunger and maternal education level and living in the Mid-West, Southeast and South. The findings show that the experience of hunger among Brazilian adolescents coexists with risky eating behaviors, body dissatisfaction, and social inequality.

RESUMO Antecedentes: A descompressão cirúrgica neural periférica (DCNP) é usada como uma terapia complementar ao tratamento clínico da neurite hansênica para preservar a função neural. Objetivo: Avaliar a longo prazo (≥ 1 ano) os resultados clínicos e funcionais da DCNP na neurite hansênica. Métodos: Este estudo transversal incluiu pacientes que estavam no pós-operatório tardio (POT) de cirurgia de descompressão dos nervos ulnares, medianos, tibiais e fibulares. Foram coletados dados socioeconômicos, epidemiológicos e clínicos. Os instrumentos utilizados foram: escala visual analógica de dor (EVA), questionário de dor neuropática 4 (DN4), escala SALSA e protocolo de avaliação neurológica simplificada. Os resultados obtidos foram comparados com os do pré-operatório (PrO) e pós-operatório de 180 dias (PO180). Resultados: Foram avaliados 246 nervos de 90 pacientes: 56,6% estavam em poliquimioterapia (PQT) e 43,3% em alta da PQT. Escores motores e intensidade da dor apresentaram melhora significante (p<0,01). Houve aumento nos escores sensitivos nos nervos ulnares bilaterais (p<0,05). Neurite ulnar foi indicação cirúrgica em 26,0% dos casos operados, seguida pela neurite tibial (23,6%). Dor neuropática foi relatada em 41% dos casos. Dose diária de prednisona reduziu de 39,6 mg (±3,0) na PrO, 16,3 mg (±5,2) na PO180, para 1,7 mg (±0,8) na POT. Escala SALSA mostrou limitação leve da atividade em 51% e moderada em 34% dos pacientes. 80% dos indivíduos relataram que os resultados atingiram suas expectativas. Conclusão: DCNP na hanseníase foi eficaz a longo prazo na redução da prevalência e intensidade da dor, na melhora da função motora e redução da dose de corticosteroides, refletindo na satisfação do paciente.

ABSTRACT Background: Peripheral neural surgical decompression (PNSD) is used as a complementary therapy to the clinical treatment of neuritis to preserve neural function. Objective: To evaluate the long-term (≥ 1 year) clinical and functional results for PNSD in leprosy neuritis. Methods: This cross-sectional study included leprosy patients who were in late postoperative period (LPO) of surgical decompression of ulnar, median, tibial, and fibular nerves. Socioeconomic, epidemiological, and clinical data were collected. The following instruments were used in this evaluation: visual analogue pain scale (VAS), Douleur Neuropathique en 4 Questions (DN4), SALSA scale, and simplified neurological assessment protocol. The preoperative (PrO) and 180-day postoperative (PO180) results were compared. Results: We evaluated 246 nerves from 90 patients: 56.6% were on multidrug therapy (MDT) and 43.3% discharged from MDT. Motor scores and pain intensity showed statistically significant improvement (p<0.01). There was an increase in sensory scores only for bilateral ulnar nerves (p<0.05). Of the operated cases, 26.0% of patients were referred for surgery of ulnar neuritis and 23.6% of tibial neuritis. Neuropathic pain was reported in 41% of cases. Daily dose of prednisone reduced from 39.6 mg (±3.0) in PrO, 16.3 mg (±5.2) in PO180, to 1.7 mg (±0.8) in LPO. The SALSA scale results showed mild activity limitation in 51% and moderate in 34% of patients. Eighty percent of individuals reported that the results reached their expectations. Conclusions: PNSD in leprosy was effective in the long term to decrease the prevalence and intensity of pain, improve motor function, and reduce the dose of corticosteroids, which is reflected in the patients’ satisfaction.

SUMMARY: The renal glomerulus is coated by fenestrated endothelial cells and externally covered by specialized epithelial cells, known as podocytes. Scanning electron microscopy becomes an important and effective tool for its studies. Normally, samples destined for scanning microscopy are covered with a thin metallic layer. However, this step can be dispensed for some analyzes. We aimed to compare coated and uncoated samples for evaluation of the glomerular morphology of the Wistar rat kidney. Cortical region of the kidney of the 5month-old male Wistar rats were used. The fragments followed the routine procedure for scanning electron microscopy processing. Half of 10 fragments were coated with palladium gold and the remaining were not coated. Auriga Compact FIB - SEM scanning electron microscope was used to observe the samples. Different increases and voltages was evaluated. For the uncoated samples, when using voltages of 2 KV (or higher) a great charging was observed, impairing the use of such voltage. Thus, these samples were always observed under voltage of 0.5 KV. On the other hand, in the coated samples, the use of 2 KV was adequate. Almost as a consequence, in the coated samples, the podocyte structures were better characterized, generating better images. Inversely, in the uncoated samples, it was possible to visualize the desired structures and to detect the morphological characteristics of these. The results showed that it is possible to use kidney samples without previous coating to evaluate the glomerular morphology at the ultrastructural level, serving as a tool in the study of pathologies.

RESUMEN: El glomérulo renal está recubierto por células endoteliales fenestradas y cubierto externamente por células epiteliales especializadas, conocidas como podocitos. La microscopía electrónica de barrido se convierte en una herramienta importante y efectiva para sus estudios. Normalmente, las muestras destinadas a microscopía de barrido se cubren con una capa metálica delgada. Sin embargo, este paso se puede dispensar para algunos análisis. El objetivo fue comparar muestras recubiertas y no recubiertas para evaluar la morfología glomerular del riñón de rata Wistar. Se utilizó la región cortical del riñón de ratas Wistar macho de 5 meses de edad. Se realizó el procedimiento de rutina para el procesamiento de microscopía electrónica de barrido. La mitad de 10 fragmentos se recubrieron con oro paladio y los restantes no se recubrieron. Se utilizó un microscopio electrónico de barrido SEM Auriga Compact FIB para observar las muestras. Se evaluaron diferentes aumentos y voltajes. Para las muestras no recubiertas, al usar voltajes de 2 KV (o más) se observó una gran carga, impidiendo el uso de dicho voltaje. Por lo tanto, estas muestras siempre se observaron a bajo voltaje de 0,5 KV. Por otro lado, en las muestras recubiertas, el uso de 2 KV fue adecuado. Como consecuencia, en las muestras recubiertas, las estructuras de los podocitos se caracterizaron mejor, generando mejores imágenes. Inversamente, en las muestras no recubiertas, fue posible visualizar las estructuras deseadas y detectar las características morfológicas de éstas. Los resultados mostraron que es posible utilizar muestras de riñón sin recubrimiento previo para evaluar la morfología glomerular a nivel ultraestructural, que sirve como una herramienta en el estudio de patologías.

BACKGROUND The longstanding application of insecticides for vector control without periodic monitoring of the population response to these chemicals can directly drive the selection of resistant populations of vector mosquitoes. Tocantins is the newest state of the Brazilian Federation. Despite a historically high number of dengue cases, studies and monitoring data concerning insecticide resistance in the state are lacking. OBJECTIVES To verify the resistance status of Aedes aegypti from 10 populations distributed throughout the state connected by rivers and roads. METHODS Between 50 and 150 ovitraps were installed in house gardens within each municipality. Collection points were established based on the importance of the towns and on geographic aspects. Dose response bioassays were performed in accordance with World Health Organization guidelines. Molecular assays were conducted to detect kdr mutations, which are related to pyrethroid resistance. FINDINGS Of the 3,200 ovitrap paddles analysed, 25.8% contained eggs, with a total of 55,687 eggs collected. With the exception of Caseara, all evaluated populations were considered to be resistant to temephos. The data showed different levels of resistance to deltamethrin among the samples. Caseara and Guaraí showed the lowest RR95 values. On average, the NaVR1 kdr allele was most frequent (40.3%), followed by NaVS (38.1%), and NaVR2 (21.6%). Palmas, the capital of the state, had the highest frequency of kdr alleles (87.5%). MAIN CONCLUSIONS With the exception of Palmas, the towns with the highest indexes (ovitrap positivity, number and density of eggs), as well with high levels of resistance and kdr alleles were located along the BR-153 road, indicating that the flow of people and cargo can contribute to the dispersion of the vector and potentially resistance. This study contributes substantially to knowledge regarding the insecticide resistance profile of Tocantins mosquito populations; the data generated via the study could facilitate the judicious use of insecticides by vector control programs.

ABSTRACT Purpose: The purpose of our study was to evaluate the clinical impact of 68Ga-PSMA PET / CT in the setting of biochemical recurrence of prostate cancer. Materials and Methods: We retrospectively evaluated 125 prostate cancer patients submitted to the 68Ga-PSMA PET / CT due to biochemical recurrence. The parameters age, Gleason score, PSA levels, and the highest SUVmax were correlated to potential treatment changes. The highest SUVmax values were correlated with age and Gleason score. The median follow-up time was 24 months. Results: 68Ga-PSMA PET / CT led to a treatment change in 66 / 104 (63.4%) patients (twenty-one patients were lost to follow-up). There was a significant change of treatment plan in patients with a higher Gleason score (P = 0.0233), higher SUVmax (p = 0.0306) and higher PSA levels (P < 0.0001; median PSA = 2.55 ng / mL). Conclusion: 68Ga-PSMA PET / CT in prostate cancer patients with biochemical recurrence has a high impact in patient management.

This study presents the toxicological effects of iron nanoparticles (NP Fe2O3) using in vitro and in vivo tests. Initially nanoparticles were characterized physic-chemically followed by evaluation of cell viability using different cell lines. Allium cepa test and comet assay (cell lines and Danio rerio) were used for evaluation of genotoxicity. Oxidative stress analyses were performed using D. rerio exposed to NP-Fe2O3, and the enzymatic activity of the enzymes catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST) were evaluated. The results showed that initially nanoparticles had 65.55 nm, 0.24 of polydispersity of and 11.4 mV of zeta potential. Regarding cell viability, it was observed that this did not reach the IC50 up to the concentration 1 x 1010 NP mL-1. The in vitro comet assay showed that in concentrations 1.96 x108 and 109 NPs mL-1 these presented toxicity, for Allium cepa evaluation in concentrations 19.6 and 39.0 x109 NPs mL-1 presented significant damages when compared to the control. Oxidative stress showed that the liver was the most affected organ when compared to the control. Although studies show that iron nanoparticles do not lead to changes, further studies are needed to make sure they do not lead to environmental changes.