ABSTRACT Custard apple (Annona squamosa L.) is a sensitive fruit crop to salinity. One of the alternatives to minimize the deleterious effects of salt stress is adopting mineral fertilization management practices, promoting increased crop tolerance to salts. Therefore, the objective of the present study was to evaluate NPK doses to mitigate salt stress in custard apple seedlings irrigated with saline wastewater. The experiment was performed in a greenhouse, adopting a randomized block design in a 3 × 5 factorial scheme, corresponding to three irrigation waters (local-supply water of 0.53 dS m-1 electrical conductivity (control); desalination reject of 3.5 dS m-1; fish farming effluent of 3.5 dS m-1) and five proportions of the NPK recommendation (25, 50, 75, 100, and 125% of the fertilization recommendation), with four replicates. The growth, biomass accumulation, and nutrients were evaluated. Irrigation with desalination reject, and fish farming effluent restricted the growth of custard apple seedlings, regardless of the NPK dose used. The ionic homeostasis of seedlings irrigated with desalination reject and fish farming effluent is optimized by fertilization with 50% of the NPK recommendation corresponding to 50, 150, and 75 mg of N, P2O5, and K2O dm-3, respectively. For the production of seedlings irrigated with local-supply water, the dose of 75% NPK, corresponding to 75, 225, and 112.5 mg of N, P2O5, and K2O dm-3, respectively, is recommended. Annona L. L salinity practices salts Therefore wastewater greenhouse scheme localsupply local supply 053 0 53 0.5 m1 m 1 m- control (control) 35 3. 25, 25 (25 50 100 125 recommendation, , recommendation) replicates accumulation evaluated used 150 7 N P2O5 PO P O KO K dm3, dm3 dm 3, dm-3 respectively 225 1125 112 112. recommended 05 0. (control 2 (2 10 12 15 P2O dm- 22 11 (

RESUMO A pinheira (Annona squamosa L.) é uma frutífera sensível à salinidade. Uma das alternativas para minimizar os efeitos deletérios do estresse salino é a adoção de práticas de manejo da fertilização mineral, promovendo o aumento da tolerância da cultura aos sais. Portanto, o objetivo foi avaliar as doses de NPK para mitigar o estresse salino em mudas de pinheira irrigadas com água residual salina. O experimento foi realizado em casa de vegetação, em delineamento de blocos casualizados, em esquema fatorial 3 × 5, correspondendo a três águas de irrigação (água de abastecimento local de 0,53 dS m-1 de condutividade elétrica (controle); rejeito salino da dessalinização de 3,50 dS m-1; efluente de piscicultura de 3,50 dS m-1) e cinco proporções da recomendação de NPK (25, 50, 75, 100 e 125% da recomendação de fertilização), com quatro repetições. O crescimento, acúmulo de biomassa e nutrientes foram avaliados. A irrigação com rejeito salino e efluente de piscicultura restringe o crescimento das mudas de pinha, independentemente da dose de NPK utilizada. A homeostase iônica das mudas irrigadas com rejeito salino e efluente de piscicultura é otimizada pela fertilização com 50% da recomendação de NPK correspondente a 50, 150 e 75 mg de N, P2O5 e K2O dm-3, respectivamente. Para a produção de mudas irrigadas com água de abastecimento local, recomenda-se a dose de 75% de NPK, correspondente a 75, 225 e 112,5 mg de N, P2O5 e K2O dm-3, respectivamente. Annona L. L salinidade mineral sais Portanto salina vegetação casualizados 5 053 0 53 0,5 m1 m 1 m- controle (controle) 350 50 3,5 25, 25 (25 10 125 fertilização, , fertilização) repetições avaliados pinha utilizada 15 7 N PO P P2O KO K dm3, dm3 dm 3, dm-3 respectivamente recomendase recomenda se 22 1125 112 112, 05 0, (controle 35 2 (2 12 dm- 11 (

Abstract Natural History Collections (NHCs) represent the world’s largest repositories of long-term biodiversity datasets. Specimen collection and voucher deposition has been the backbone of NHCs since their inception, but recent decades have seen a drastic decline in rates of growth via active collecting. Amphibians and reptiles are amongst the most threatened zoological groups on the planet and are historically underrepresented in most worldwide NHCs. As part of an ongoing project to review the Portuguese zoological collections in the country’s NHCs, herpetological data from its three major museums and smaller collections was gathered and used to examine the coverage and representation of the different taxa extant in Portugal. These collections are not taxonomically, geographically, or temporally complete. Approximately 90% of the Portuguese herpetological taxa are represented in the country’s NHCs, and around half of the taxa are represented by less than 50 specimens. Geographically, the collections cover less than 30% of the country’s territory and almost all of the occurring taxa have less than 10% of their known distribution represented in the collections. A discussion on the implications for science of such incomplete collections and a review of the current status of Portuguese NHCs is presented. (NHCs worlds world s longterm long term datasets inception collecting countrys country Portugal taxonomically geographically complete 90 5 specimens Geographically 30 10 presented 9 3 1

To investigate the anthropogenic contamination of the Port of Suape, five surface sediments were collected and subjected to a geochemical characterization based on the determination of environmental biomarkers such as sterols, aliphatic and polycyclic aromatic hydrocarbons, and also metals and arsenic. Sterol analyses point to a moderate level of sewage contamination, while n-alkanes analyses indicated contamination by petroleum in an early stage of biodegradation. Polycyclic aromatic hydrocarbons analyses pointed to a mixture of contributions for the organic matter, which were predominantly found for petrogenic and pyrolytic sources. The quantification of trace metals indicated a low risk to the environment, except for As, which was identified as displaying moderate contamination. During the analysis of all biomarkers, the sediment collected near one of the shipyards was the most affected. These results enable comprehension of the level of contamination in an important Brazilian port and the need to develop remediation policies. Suape sterols arsenic nalkanes n alkanes biodegradation matter sources environment As affected policies

ABSTRACT Sporotrichosis is a neglected mycosis that affects human and animal hosts, including domestic cats. In Brazil, its most frequently diagnosed etiological agent is Sporothrix brasiliensis. Zoonotic transmission of S. brasiliensis occurs via direct contact between an infected cat and a susceptible human host. Notification of confirmed cases of feline sporotrichosis is not mandatory in Brazil. The metropolitan area of Goiania city can be considered a silent area for the occurrence of feline sporotrichosis. In this context, voluntary reporting of feline sporotrichosis cases is recommended for all healthcare professionals. This study aimed to report the first occurrence of S. brasiliensis in a cat from the metropolitan area of Goiania city. Cytopathology, mycology, thermal dimorphism and calmodulin gene amplification tests were performed. The mycological and molecular biological diagnoses corresponded to S. brasiliensis. The etiological agent of zoonotic sporotrichosis was detected in the metropolitan area of Goiania city, and therefore there is a risk of the emergence of new cases of cats infected with S. brasiliensis and the occurrence of zoonotic transmission of this fungus. hosts Brazil S host context professionals Cytopathology mycology performed fungus

ABSTRACT Noroviruses are highly infectious, genetically diverse viruses. Global outbreaks occur frequently, making molecular surveillance important for infection monitoring. This cross-sectional descriptive study aimed to monitor cases of norovirus gastroenteritis in the Brazilian Amazon. Fecal samples were tested by immunoenzymatic assay, RT-PCR and genetic sequencing for the ORF1/ORF2 and protease regions. Bayesian inference with a molecular clock was employed to construct the phylogeny. The norovirus prevalence was 25.8%, with a higher positivity rate among children aged 0-24 months. Genogroup GII accounted for 98.1% of the sequenced samples, while GI accounted for 1.9% of them. The GII.P16/GII.4 genotype was the most prevalent, with an evolution rate of 2.87x10−3 and TMRCA estimated in 2012. This study demonstrates that norovirus is a primary causative agent of gastroenteritis and provides data on viral genetic diversity that may facilitate infection surveillance and vaccine development. infectious viruses frequently monitoring crosssectional cross sectional Amazon assay RTPCR RT PCR ORF1ORF2 ORFORF ORF1 ORF2 ORF ORF1/ORF regions phylogeny 258 25 8 25.8% 024 0 24 0-2 months 981 98 1 98.1 19 9 1.9 them GIIP16GII4 GIIPGII P16 4 P GII.P16/GII. prevalent 287x103 x 2 87x10 3 2.87x10− 2012 development ORF1ORF 25.8 02 0- 98. 1. GIIP GIIP16GII P1 GII.P16/GII 287x10 87x1 2.87x10 201 25. 287x1 87x 2.87x1 20 287x 2.87x

ABSTRACT Objective: To evaluate the cumulative incidence, risk factors, and outcomes of COVID-19 in patients with Cushing's disease (CD). Subjects and methods: In all, 60 patients with CD following up in our outpatient clinic answered via phone interview a questionnaire about the occurrence of COVID-19 infection documented by RT-PCR (including the diagnosis date and clinical outcome) and vaccination status. Clinical and biochemical data on disease activity (hypercortisolism) and comorbidities (obesity, diabetes mellitus, and hypertension) were obtained from the patients' electronic medical records. Risk ratios (RRs) of risk factors were obtained using univariate and multivariate analyses. Results: The cumulative incidence of COVID-19 in patients with CD during the observation period was 31.7%, which was higher than that in the general reference population (9.5%). The cumulative incidence of COVID-19 was significantly higher in patients with hypercortisolism (57% versus 17% in those without hypercortisolism, p = 0.012) and obesity (54% versus 9% in those without obesity, p < 0.001) but not in patients with hypertension or diabetes mellitus. On multivariate analysis, hypercortisolism and obesity were each independent risk factors for COVID-19 (RR 2.18, 95% CI 1.06-4.46, p = 0.033 and RR 5.19, 95% CI 1.61-16.74, p = 0.006, respectively). Conclusion: The incidence of COVID-19 in patients with CD was associated with hypercortisolism, as expected, and obesity, a novel and unexpected finding. Thus, correction of hypercortisolism and obesity should be implemented in patients with CD during the current and future COVID-19 outbreaks. Objective COVID19 COVID 19 COVID-1 Cushings Cushing s CD. . (CD) methods all 6 RTPCR RT PCR including outcome status (hypercortisolism (obesity mellitus records RRs (RRs analyses Results 317 31 7 31.7% 9.5%. 95 9.5% 9 5 (9.5%) 57% 57 (57 17 0.012 0012 0 012 54% 54 (54 0.001 0001 001 analysis 218 2 18 2.18 1.064.46, 106446 1.06 4.46, 1 06 4 46 1.06-4.46 0033 033 0.03 519 5.19 1.6116.74, 1611674 1.61 16.74, 61 16 74 1.61-16.74 0006 006 0.006 respectively. respectively respectively) Conclusion expected finding Thus outbreaks COVID1 COVID- (CD 3 31.7 9.5 (9.5% (5 0.01 01 0.00 000 00 21 2.1 064 1.064.46 10644 106 1.0 446 4.46 1.06-4.4 003 03 0.0 51 5.1 6116 1.6116.74 161167 161 1.6 1674 16.74 1.61-16.7 31. 9. (9.5 ( 2. 1.064.4 1064 10 1. 44 4.4 1.06-4. 0. 5. 611 1.6116.7 16116 167 16.7 1.61-16. (9. 1.064. 4. 1.06-4 1.6116. 1611 16. 1.61-16 (9 1.064 1.06- 1.6116 1.61-1 1.611 1.61-

Resumo Salmonella spp. é uma das principais causas de gastroenterite em todo o mundo. Salmonella Heidelberg é um patógeno emergente associado com surtos com multirresistência antimicrobiana vinculados aos produtos avícolas. A sua alta persistência no ambiente pode estar associada com sua habilidade de aderir a diferentes superfícies e formar biofilmes. Devido ao aumento da resistência antimicrobiana em todo o mundo, os pesquisadores têm investigado o uso de bactérias acido láticas (BAL) como um controle biológico e de microrganismos patogênicos. O objetivo deste estudo foi avaliar a habilidade de BAL no controle de biofilmes produzidos por S. Heidelberg em placas de poliestireno. Foi avaliada a atividade antimicrobiana in vitro de nove BAL, todas pertencentes ao gênero Lactobacillus, na inibição e na remoção de biofilmes produzidos por S. Heidelberg. A formação de biofilme só ocorreu quando a BAL1 (Lactobacillus salivaris) foi utilizada. Todos os outros tratamentos demonstraram atividade antimicrobiana. Entretanto, a BAL não foi capaz de reduzir a contagem bacteriana. Os resultados obtidos demonstram que BAL são capazes de prevenir ou retardar a formação de biofilme por S. Heidelberg em superfícies de poliestireno e podem ser utilizadas em estudos in vivo para determinar o seu potencial alternativo no controle deste patógeno na indústria de alimentos. spp mundo avícolas (BAL patogênicos S Lactobacillus salivaris utilizada Entretanto bacteriana alimentos

Abstract Salmonella spp. is one of the leading causes of gastroenteritis worldwide. Salmonella Heidelberg is an emergent pathogen associated with multidrug-resistant outbreaks linked to poultry products. Their high persistence in the environment may be associated with their ability to adhere to different surfaces and form biofilms. Owing to increased antimicrobial resistance worldwide, researchers have investigated the use of lactic acid bacteria (LAB) as a biological control against pathogenic microorganisms. This study aimed to evaluate the ability of LAB to control the formation of S. Heidelberg biofilms on polystyrene surfaces. The antibiofilm activity of nine LAB strains, all belonging to Lactobacillus genera, related to the inhibition of biofilms produced by S. Heidelberg was evaluated in vitro. All treatments, except LAB1 (Lactobacillus salivaris), showed antibiofilm activity. However, LAB did not reduce bacterial counts. Our results show that LAB can avoid or delay biofilm formation by S. Heidelberg on polystyrene surfaces and may be used for in vivo studies as a potential alternative to help control this pathogen in food industries. spp worldwide multidrugresistant multidrug resistant products (LAB microorganisms S strains genera vitro treatments salivaris, salivaris , salivaris) However counts industries

ABSTRACT Objective: This study aims to investigate the ability of the six-minute walk distance (6MWD) as a prognostic marker for midterm clinical outcomes three months after coronary artery bypass grafting (CABG), to identify possible predictors of fall in 6MWD in the early postoperative period, and to establish the percentage fall in early postoperative 6MWD, considering the preoperative baseline as 100%. Methods: A prospective cohort of patients undergoing elective CABG were included. The percentage fall in 6MWD was assessed by the difference between preoperative and postoperative day (POD) five. Clinical outcomes were evaluated three months after hospital discharge. Results: There was a significant decrease in 6MWD on POD5 compared with preoperative baseline values (percentage fall of 32.5±16.5%, P<0.0001). Linear regression analysis showed an independent association of the percentage fall of 6MWD with cardiopulmonary bypass (CPB) and preoperative inspiratory muscle strength. Receiver operating characteristic curve analysis revealed that the best cutoff value of percentage fall in 6MWD to predict poorer clinical outcomes at three months was 34.6% (area under the curve = 0.82, sensitivity = 78.95%, specificity = 76.19%, P=0.0001). Conclusion: This study indicates that a cutoff value of 34.6% in percentage fall of 6MWD on POD5 was able to predict poorer clinical outcomes at three months of follow-up after CABG. Use of CPB and preoperative inspiratory muscle strength were independent predictors of percentage fall of 6MWD in the postoperative period. These findings further support the clinical application of 6MWD and propose an inpatient preventive strategy to guide clinical management over time.

ABSTRACT The objective of the present study was to evaluate the qPCR for detection and enumeration of Staphylococcus aureus and Streptococcus agalactiae using different milk samplings in comparison to the conventional microbiology. Four dairy herds with a history of subclinical mastitis caused by S. aureus and S. agalactiae were selected. Sampling approach included milk samples from bulk tank (BT), cow level (composite samples, CO), and mammary quarter level (MQ) from 785 lactating cows. Three consecutive monthly milk samplings were carried out, totaling 3347 MQ milk samples, 912 CO, and 12 from BT. All collected milk samples were subjected to conventional microbiology and qPCR for detection and enumeration of S. aureus and S. agalactiae. The qPCR showed 71.5% of diagnostic sensitivity for S. aureus isolated from MQ milk samples, 71.8% for CO, and 50% for BT milk samples compared with conventional microbiology methodology. Taken together, the diagnostic sensitivity for S. agalactiae isolated from MQ milk samples was 90.2, 87.7 for CO, and 90.9% for BT milk samples. In general, the qPCR methodology enabled the detection of S. aureus and S. agalactiae, regardless of the type of milk sampling. The direct use of milk samples to estimate the counting of S. aureus by qPCR demonstrated lower sensitivity than the counting of S. agalactiae, which can be explained by the pathogen infection dynamics and differences in milk sample type. S selected BT, , (BT) composite CO CO) (MQ 78 cows out 334 91 1 715 71 5 71.5 718 8 71.8 50 together 902 90 2 90.2 877 87 7 87. 909 9 90.9 general sampling (BT 33 71. 90. 3

Boron-manganese steel 22MnB5 is extensively used in structural automotive components. Knowledge about its microstructural evolution during hot stamping and resistance spot welding (RSW) is extremely relevant to guarantee compliance with application requirements. Particularly, corrosion properties are critical to the application of uncoated sheet steels. However, microstructural studies are usually simplified to top-hat geometries, which might not be fully representative of the complex thermomechanical cycles locally faced by a real component. Therefore, the present work brings an extensive characterization of a hot-stamped 22MnB5 automotive B-pillar in terms of microstructure, hardness and corrosion resistance, which were correlated with a reverse engineering of the process using numerical simulation. Physical simulations of the subcritical heat affected zone (SCHAZ) of RSW were done to assess the influence of microstructure on martensite tempering. Results showed that the component undergoes a complex strain distribution along its body during hot stamping. Most heavily strained regions presented higher amounts of ferrite, leading to poorer corrosion resistance, since ferrite behaves as an anode. Physical simulations of the SCHAZ showed that the softening degree due to martensite tempering is solely affected by peak temperature, while other microstructural features appear to exert negligible or no influence. Boronmanganese Boron manganese MnB 22MnB components (RSW requirements Particularly steels However tophat top hat geometries Therefore hotstamped stamped Bpillar B pillar simulation (SCHAZ anode temperature

ABSTRACT Objectives: Congenital hypothyroidism (CH) can be permanent (PCH) or transient (TCH). While the importance of thyroxine in myelination of the brain is undisputed, the benefits to neurodevelopmental outcomes of TCH treatment are controversial. Our objectives were to determine predictive factors for PCH and verify its prevalence changes over time. Subjects and methods: A total of 165 children were evaluated at 3 years of age to verify the diagnosis of PCH. 130 were submitted to a two-step cluster analysis, with the aim of grouping them into homogeneous clusters. The mean incidence of PCH and TCH was calculated from 2004 to 2010 and 2011 to 2015. Results: Sixty-six children were diagnosed with PCH, and 99 were diagnosed with TCH. Eighty-one percent of PCH children and all TCH children with thyroid imaging had glands in situ. Eighty children (61.5%) were in Cluster 1, 8 children (6.2%) were in Cluster 2 and 42 children (32.3%) were in Cluster 3. No children had PCH in Cluster 1, while 87.5% of children in Cluster 2 and all children in Cluster 3 had PCH. The most important predictor for PCH was the initial serum TSH, which was marginally higher in importance than the blood spot TSH, followed by the initial serum free T4. The mean incidence of PCH (odds ratio: 1.95, 95% CI 1.36 to 2.95, p < 0.0001) and TCH (odds ratio 1.33, 95%, CI 1.02 to 1.77, p = 0,038) increased over time. Conclusions: The most important PCH predictors are the initial serum TSH and the blood spot TSH. The mean incidence of both PCH and TCH in our series increased. Objectives CH (CH (PCH . (TCH) undisputed controversial time methods 16 13 twostep two step analysis clusters 200 201 2015 Results Sixtysix Sixty six 9 Eightyone one situ 61.5% 615 61 5 (61.5% 1 6.2% 62 6 (6.2% 4 32.3% 323 32 (32.3% 875 87 87.5 T4 T odds 195 95 1.95 136 36 1.3 295 2.95 0.0001 00001 0 0001 133 33 1.33 102 02 1.0 177 77 1.77 0,038 0038 038 Conclusions (TCH 20 61.5 (61.5 6.2 (6.2 32.3 (32.3 87. 19 1.9 1. 29 2.9 0.000 0000 000 10 17 7 1.7 0,03 003 03 61. (61. 6. (6. 32. (32. 2. 0.00 00 0,0 (61 (6 (32 0.0 0, ( (3 0.

ABSTRACT Objective: This study aims to investigate the ability of the six-minute walk distance (6MWD) as a prognostic marker for midterm clinical outcomes three months after coronary artery bypass grafting (CABG), to identify possible predictors of fall in 6MWD in the early postoperative period, and to establish the percentage fall in early postoperative 6MWD, considering the preoperative baseline as 100%. Methods: A prospective cohort of patients undergoing elective CABG were included. The percentage fall in 6MWD was assessed by the difference between preoperative and postoperative day (POD) five. Clinical outcomes were evaluated three months after hospital discharge. Results: There was a significant decrease in 6MWD on POD5 compared with preoperative baseline values (percentage fall of 32.5±16.5%, P<0.0001). Linear regression analysis showed an independent association of the percentage fall of 6MWD with cardiopulmonary bypass (CPB) and preoperative inspiratory muscle strength. Receiver operating characteristic curve analysis revealed that the best cutoff value of percentage fall in 6MWD to predict poorer clinical outcomes at three months was 34.6% (area under the curve = 0.82, sensitivity = 78.95%, specificity = 76.19%, P=0.0001). Conclusion: This study indicates that a cutoff value of 34.6% in percentage fall of 6MWD on POD5 was able to predict poorer clinical outcomes at three months of follow-up after CABG. Use of CPB and preoperative inspiratory muscle strength were independent predictors of percentage fall of 6MWD in the postoperative period. These findings further support the clinical application of 6MWD and propose an inpatient preventive strategy to guide clinical management over time. Objective sixminute six minute MWD (6MWD CABG, , (CABG) period 100 100% Methods included POD (POD five discharge Results 325165 32 5 16 32.5±16.5% P<0.0001. P00001 P P<0.0001 . 0 0001 P<0.0001) (CPB 346 34 6 34.6 area 082 82 0.82 7895 78 95 78.95% 7619 76 19 76.19% P=0.0001. P=0.0001 P=0.0001) Conclusion followup follow up time (CABG 10 32516 3 1 32.5±16.5 P0000 P<0.000 000 34. 08 8 0.8 789 7 9 78.95 761 76.19 P=0.000 3251 32.5±16. P000 P<0.00 00 0. 78.9 76.1 P=0.00 325 32.5±16 P00 P<0.0 78. 76. P=0.0 32.5±1 P0 P<0. P=0. 32.5± P<0 P=0 32.5 P< P= 32.

In recent years, technological development in analytical instrumentation has progressed vigorously, which has allowed the evolution of analytical techniques that play an indispensable role within society, highlighting mass spectrometry. Parallel to the technological advance of this technique, the development of new ionization methods that are simpler, faster, and allow direct analysis without the need for prior treatment or sample processing is underway. The methods of ambient ionization derived from paper spray have been developing rapidly in current days, mainly in the forensic field, due to the chemical modifications made in the paper substrate and the introduction of new types of organic materials that function as a probe/substrate to collect, store, and ionize the sample, then enabling more efficient and selective analyzes. This review highlights direct analyzes by applying paper spray ionization in the forensic field and some variations of this technique, including Thread Spray Ionization (TSI) when a fabric thread is used; Touch Spray Ionization (TSI), which uses materials such as metallic needles, medical swabs, and wooden sticks; Fiber Spray Ionization (FSI), which applies polymeric fibers as substrates. years vigorously society spectrometry technique simpler faster underway days probesubstrate probe collect store TSI (TSI used TSI, , needles swabs sticks FSI, FSI (FSI) substrates (FSI

Abstract The TP53 3’UTR variant rs78378222 A>C has been detected in different tumor types as a somatic alteration that reduces p53 expression through modification of polyadenylation and miRNA regulation. Its prevalence is not yet known in all tumors. Herein, we examine tumor tissue prevalence of rs7837822 in Brazilian cohorts of patients from south and southeast regions diagnosed with lung adenocarcinoma (LUAD, n=586), sarcoma (SARC, n=188) and uterine leiomyoma (ULM, n=41). The minor allele (C) was identified in heterozygosity in 6/586 LUAD tumors (prevalence = 1.02 %) and none of the SARC and ULM samples. Additionally, next generation sequencing analysis revealed that all variant-positive tumors (n=4) with sample availability had additional pathogenic or likely pathogenic somatic variants in the TP53 coding regions. Among them, 3/4 (75 %) had the same pathogenic or likely pathogenic sequence variant (allele frequency <0.05 in tumor DNA) namely c.751A>C (p.Ile251Leu). Our results indicate a low somatic prevalence of rs78378222 in LUAD, ULM and SARC tumors from Brazilian patients, which suggests that no further analysis of this variant in the specific studied regions of Brazil is warranted. However, these findings should not exclude tumor molecular testing of this TP53 3’UTR functional variant for different populations. TP TP5 3UTR UTR 3 rs AC A C p p5 regulation Herein rs783782 (LUAD n=586, n586 n n=586 , 586 n=586) SARC, (SARC n=188 n188 188 ULM, (ULM n=41. n41 n=41 . 41 n=41) (C 6586 6 6/58 102 1 02 1.0 % samples Additionally variantpositive positive n=4 n4 4 (n=4 them 34 3/ 75 (7 005 0 05 <0.0 DNA c751AC cAC c 751A p.Ile251Leu. pIle251Leu pIleLeu p.Ile251Leu Ile251Leu Ile Leu (p.Ile251Leu) warranted However populations rs78378 n58 n=58 58 n=18 n18 18 658 6/5 10 1. n= (n= 7 ( 00 <0. pIle IleLeu (p.Ile251Leu rs7837 n5 n=5 5 n=1 n1 65 6/ (n <0 rs783 < rs78 rs7

ABSTRACT Background: The World Health Organization recommends a market-ready, urine-based point-of-care diagnostic test for circulating cathodic antigens (CCA) to determine the prevalence of S. mansoni. This study evaluated the performance of the URINE CCA (SCHISTO) ECO TESTE® (POC-ECO), which is currently available in Brazil. Methods: Residents from eight sites with different prevalence estimates provided one urine sample for POC-ECO and one stool sample for Kato-Katz (KK) and Helmintex® (HTX) testing as an egg-detecting reference for infection status. Results: None of the study sites had significantly higher POC-ECO accuracy than KK. Conclusions: POC-ECO is not currently recommended in Brazilian schistosomiasis elimination programs. Background marketready, marketready market ready, ready market-ready urinebased based pointofcare point care (CCA S mansoni SCHISTO (SCHISTO TESTE POCECO, POCECO POC , (POC-ECO) Brazil Methods KatoKatz Kato Katz KK (KK Helmintex HTX (HTX eggdetecting egg detecting status Results Conclusions programs (POC-ECO