Resumo O objetivo deste estudo foi determinar a atividade alergênica de componentes presentes em extratos crus de plerocercos (CPE) e de blastocistos de Pterobothrium crassicolle (CBE), obtidos de Micropogonias furnieri, em modelo murino. Dois grupos de sete animais receberam cada um 50 µg de CPE ou CBE nos dias 1, 35 e 120. As amostras de soro foram testadas por ELISA e Imunoblot. Os níveis específicos de IgG e IgE foram detectados por ELISA, mostrando respostas humorais específicas para a imunização primária para ambas as imunoglobulinas e títulos crescentes de IgE. Testes positivos de Anafilaxia Cutânea Passiva em ratos sensibilizados com soros anti-CBE e testados por CBE, demonstraram biologicamente, a atividade alergênica dos extratos. O CPE e o CBE evidenciaram algumas regiões de reconhecimento diferentes, mas ambos os grupos experimentais reconheceram todas as regiões dos extratos, quando testados para reações cruzadas, mostrando que o CPE e o CBE poderiam compartilhar locais de reconhecimento antigênico. (CPE , (CBE) furnieri murino 5 1 3 120 Imunoblot antiCBE anti biologicamente diferentes cruzadas antigênico (CBE 12

Abstract The aim of this study was to determine the allergenic activity of components present in crude extracts of Pterobothrium crassicolle plerocerci (CPE) and blastocysts (CBE) obtained from Micropogonias furnieri in a murine model. Two groups of seven animals each received 50 µg of CPE or CBE on days 1, 35 and 120. Serum samples were tested by ELISA and Immunoblotting. Specific IgG and IgE levels were detected by ELISA, showing specific humoral responses for the primary immunization for both immunoglobulins and continuously growing titers for IgE. Positive Passive Cutaneous Anaphylaxis tests in rats sensitized with anti-CBE sera and tested by CBE, showed biologically, the allergenic activity of the extracts. The CPE and CBE showed some different recognition regions but both experimental groups recognized all regions of the extracts when tested for cross reactions, showing that CPE and CBE could share antigenic recognition sites. (CPE (CBE model 5 1 3 120 Immunoblotting antiCBE anti biologically reactions sites 12

Resumo Alguns parasitos de peixes podem causar uma variedade de sintomas em humanos, incluindo alergias. Este foi um estudo transversal baseado em entrevistas, análise sérica por ELISA para IgG anti–Pterobothrium heteracanthum e avaliação estatística. Quatro indivíduos foram sororreativos (6,25%), sem associação com o manuseio dos peixes (p = 1,000) ou com a ingestão maior que duas vezes por semana (p = 0,232). Houve associação significativa (p=0,032) entre reatividade e ausência de sintomas alérgicos. A sororreatividade contra P. heteracanthum em humanos foi detectada, mas não foi associada à quantidade de peixe ingerido, manuseado ou a queixas alérgicas. alergias entrevistas antiPterobothrium anti Pterobothrium estatística 6,25%, 625 6,25% , 6 25 (6,25%) p 1,000 1000 1 000 0,232. 0232 0,232 . 0 232 0,232) p=0,032 p0032 032 (p=0,032 alérgicos P detectada ingerido alérgicas 62 6,25 2 (6,25% 1,00 100 00 023 0,23 23 p=0,03 p003 03 (p=0,03 6,2 (6,25 1,0 10 02 0,2 p=0,0 p00 (p=0,0 6, (6,2 1, 0, p=0, p0 (p=0, (6, p=0 (p=0 (6 p= (p= (

Abstract Some fish parasites can cause a variety of symptoms in humans, including allergies. This was a cross-sectional study based on interviews, serum analysis by ELISA for anti–Pterobothrium heteracanthum IgG and a statistical evaluation. Four individuals were seroreactive (6.25%), with no association with fish handling (p = 1.000) or with ingestion more than twice a week (p = 0.232). There was a significant association (p = 0.032) between reactivity and the absence of allergy symptoms. Seroreactivity against P. heteracanthum in humans was detected, but was not associated with the amount of fish ingested, handled, or with allergic complaints. allergies crosssectional cross sectional interviews antiPterobothrium anti Pterobothrium evaluation 6.25%, 625 6.25% , 6 25 (6.25%) p 1.000 1000 1 000 0.232. 0232 0.232 . 0 232 0.232) 0.032 0032 032 P detected ingested handled complaints 62 6.25 2 (6.25% 1.00 100 00 023 0.23 23 0.03 003 03 6.2 (6.25 1.0 10 02 0.2 0.0 6. (6.2 1. 0. (6. (6 (

O objetivo deste estudo foi determinar as especies de Anisakidae parasitando congro-rosa, Genypterus brasiliensis Regan, 1903 comercializados nos mercados dos municipios de Niterói e Rio de Janeiro, Brasil, de interesse em saúde pública, enfatizando seus índices parasitarios, sitios de infecção e importância higiénico-sanitária. Setenta e quatro espécimes de G. brasiliensis foram necropsiados de outubro de 2002 a setembro de 2003. Vinte peixes (27%) estavam parasitados por larvas vivas de nematóides Anisakidae: Anisakis physeteris, Anisakis simplex, Anisakis sp., Pseudoterranova decipiens, Pseudoterranova sp.,Hysterothylacium sp., Raphidascaris sp., Contracaecum sp. e Terranova sp. As especies Anisakis sp. e A. simplex foram as especies que apresentaram maior prevalência, ambos com 13,5% e A. physeteris a menor prevalência 1,35%. As demais especies, Pseudoterranova sp., P decipiens, Hysterothylacium sp., Raphidascaris sp., Contracaecum sp. e Terranova sp. apresentaram as seguintes prevalências 10,8%, 5,4%, 2,7%, 4,1%, 1,4% e 1,4%, respectivamente. Os sitios de infecção foram o mesentério, serosa do estómago e intestinal, instestino, ovario e musculatura.

The aim of this study was to identify the species Anisakidae, of interest to public health, parasitizing the cusk-eel, Genypterus brasiliensis Regan, 1903 purchased in markets ofNiteroi and Rio de Janeiro counties, Brazil, emphasizing their parasite indexes, sites of infection and higienic-sanitary importance. Seventy-four specimens ofG brasiliensis were necropsiedfrom October 2002 to September 2003. Twenty fish specimens (27%>) were parasitized by live larvae of Anisakidae. The collected species were represented by Anisakis physeteris, Anisakis simplex, Anisakis sp., Pseudoterranova decipiens, Pseudoterranova sp., Hysterothylacium sp., Raphidascaris sp., Contracaecum sp. and Terranova sp. The species Anisakis sp. and A. simplex presented the higher prevalences, both with 13,5% whereas A. physeteris presented the lower prevalence 1,35%. The other species, Pseudoterranova sp., P. decipiens, Hysterothylacium sp., Raphidascaris sp., Contracaecum sp. and Terranova sp. presented prevalences of 10,8%, 5,4%, 2,7%, 4,1%, 1,4% and 1,4%, respectively. The sites of infection were, the mesentery, stomach and intestinal seroses, intestine, ovary and musculature.

Specimes of elasmobranch fishes, captured in 1998 in the the State of Paraná, Brazil, were parasitized with Trypanorhyncha/Homeacanthoidea cestodes: Carcharhinus signatus with Heteronybelinia yamagutii (Dollfus, 1960) Palm, 1999 and H. nipponica (Yamaguti, 1952) Palm, 1999; Dipturus trachydermus (Krefft & Stehmann, 1975) with Mixonybelinia beveridgei (Palm et al, 1997) Palm, 1999. Details of scolex morphology of the two species of Heteronybelinia that expanded their desciptions were obtained. The known geographical distribution of the species is widened

Espécimes de peixes elasmobrânquios, capturados em 1998 no Estado do Paraná, Brasil, estavam parasitados com cestóides Trypano-rhyncha/Homeacanthoidea: Carcharhinus signatus com Heteronybelinia yamagutii e H. nipponica; Dipturus trachydermus com Mixonybelinia beveridgei Das duas espécies de Heteronybelinia foram obtidos detalhes da morfologia do escólex, que expandiram suas descrições. A distribuição geográfica das espécies foi ampliada

Twenty-nine specimes (32.2%) of elasmobranch fishes, out of 90 captured in the south coast of Brazil in the States of Paraná and Santa Catarina, represented by six families, seven genera and nine species, were parasitized with cestodes of the order Trypanorhyncha: Heptranchias perlo and Squalus sp. parasitized with Progrillotia dollfusi; Isurus oxyrinchus with Nybelinia lingualis and Gymnorhynchus isuri; Prionace glauca with Tentacularia coryphaenae, Hepatoxylon trichiuri, Molicola horridus, Floriceps saccatus and Callitetrarhynchus gracilis; Carcharhinus signatus with Heteronybelinia yamagutii, H. nipponica and P. dollfusi; C. longimanus and C. obscurus with T. coryphaenae; Sphyrna zygaena with Heteronybelinia rougetcampanae and Callitetrarhynchus speciosus; Dipturus trachydermus with Mixonybelinia beveridgei. Prevalences and intensities of infection are presented as well as the analysis of the hosts sex-related parasitism, in single or concomitant infections. Data are discussed and compared with previous available reports on these hosts, mainly those referring to the species I. oxyrinchus, P. glauca and C. longimanus

New records for helminth species recovered from elasmobranch fishes in Brazil are established. Digenean and acanthocephalan parasites of elasmobranch fishes are reported from the southern coast of Brazil: Otodistomum veliporum (Creplin, 1837) Stafford, 1904 (Digenea: Azygiidae) in the stomach and spiral valve of Dipturus trachydermus and in the spiral valve of Squatina sp. Cystacanths and juveniles of the acanthocephalans Corynosoma australe Johnston, 1937 and Corynosoma sp., in the spiral valve of Squatina sp., Galeorhinus galeus and Hexanchus griseus and in the stomach of Squalus megalops; a juvenile of Gorgorhynchus sp., in the spiral valve of Sphyrna zygaena. Dipturus trachydermus and Squatina sp. are new host records for O. veliporum. Digeneans and acanthocephalans are reported for the first time parasitizing elasmobranch fishes in Brazil.

Foi registrada prevalência de 86,4% e intensidade média de infecção de 9,1 de Hepatoxylon trichiuri em espécimes de Prionance glauca, capturados do litoral do estado do Rio Grande do Sul e prevalência de 4,9% e a intensidade de infecção de 1,0 em espécimes de Coryphaena hippurus, coletados de um mercado de peixes do estado do Rio de Janeiro. Este foi o primeiro registro de H. trichiuri em P. glauca no Brasil.

The prevalence was 86.4 % and mean intensity of infection was 9.1 of Hepatoxylon trichiuri of Prionace glauca, captured of the coastal waters of state of Rio Grande do Sul, and the prevalence was 4.9% and intensity of infection was 1.0 of Coryphaena hippurus, collected from a fish market of state of Rio de Janeiro. Hepatoxylon trichiuri was recorded by the first time of Prionace glauca in Brazil.

New records for nematode species recovered from elasmobranch fishes in Brazil are established and new systematical arrangements proposed. Parascarophis sphyrnae Campana-Rouget, 1955 from the spiral valve of Sphyrna zygaena is referred for the first time in South America as a new host record. Procamallanus (S.) pereirai Annereaux, 1946, from the spiral valve of Raja castelnaui is reported parasitizing an elasmobranch host. Nematode larvae of the genera Anisakis, Contracaecum, Pseudoterranova and Raphidascaris are listed from the stomach and spiral valves of several hosts. Anisakidae larvae previously referred in Brazil in the genus Phocanema should be reallocated in Pseudoterranova. Nematodes of the genera Anisakis, Contracaecum, Pseudoterranova and Raphidascaris are reported for the first time parasitizing elasmobranchs in Brazil.

The present study describes for the first time Ichthyophthirius multifilis infecting twenty ornamental fishes Gasteropelecus sternicola (white butterfly fish). Parasites were found in 100% of examined fishes. Light microscopy and scanning electron microscopy (SEM) were used in this exam. SEM showed fins destroyed by the parasites action. High prevalence of the parasitism has a high concern as those fishes are of commercial importance for Brazil.