ABSTRACT Sea turtles are endangered animals that present cosmopolitan distribution. Anthropic actions have been considered important causes for the reduction of sea turtle population, but natural aspects such as parasitism may also contribute to their decline. This study aimed to report the occurrence of parasites in stranded dead sea turtles found in an area known as Potiguar Basin, northeastern Brazil, from 2010 to 2019. They were identified and classified according to the carapace length. At post-mortem analyses all organs were examined, parasites collected and morphologically identified. Ecological parasitic indexes as prevalence (P), mean intensity (MI) and mean abundance (MA) were calculated. A total of 80 Chelonia mydas and 5 Eretmochelys imbricata were assessed. Neoctangium travassosi was detected in both species presenting P = 20%, MI = 4.19 and MA = 0.84 for C. mydas and P = 60%, MI = 1.67 and MA = 1.0 for E. imbricata. This is the first report of N. travassosi parasitizing E. imbricata in South America. Finally, the retrieval of these parasites is a warning regarding the need for further studies to assess the impact of this parasitism on the health and conservation of sea turtles. distribution population decline Basin Brazil 201 2019 length postmortem post mortem examined P, , (P) (MI (MA calculated 8 assessed 20 20% 419 4 19 4.1 084 0 84 0.8 C 60 60% 167 1 67 1.6 10 1. E N America Finally (P 2 41 4. 08 0. 6 16

RESUMO As tartarugas marinhas são animais ameaçados de extinção que apresentam distribuição cosmopolita. Ações antrópicas têm sido consideradas causas importantes para a redução da população de tartarugas marinhas, mas aspectos naturais, como o parasitismo, também, podem contribuir para o seu declínio. Este estudo objetivou relatar a ocorrência de parasitos em tartarugas marinhas, encalhadas mortas, encontradas em uma área conhecida como Bacia Potiguar, nordeste do Brasil, de 2010 a 2019. Essas foram identificadas e classificadas de acordo com o comprimento da carapaça. Nas análises post mortem, todos os órgãos foram examinados, e os parasitos coletados e identificados morfologicamente. Foram calculados índices parasitários ecológicos, como prevalência (P), intensidade média (IM) e abundância média (AM). Um total de 80 Chelonia mydas e cinco Eretmochelys imbricata foi estudado. Neoctangium travassosi foi detectado em ambas as espécies, apresentando P = 20%, MI = 4,19 e MA = 0,84 para C. mydas e P = 60%, IM = 1,67 e AM = 1,0 para E. imbricata. Este é o primeiro relato de N. travassossi parasitando E. imbricata na América do Sul. Conclui-se que a recuperação desses parasitos soa como um alerta para a necessidade de mais estudos para avaliar o impacto desse parasitismo na saúde e na conservação das tartarugas marinhas. cosmopolita naturais também declínio mortas Potiguar Brasil 201 2019 carapaça mortem examinados morfologicamente ecológicos P, , (P) (IM AM. . (AM) 8 estudado espécies 20 20% 419 4 19 4,1 084 0 84 0,8 C 60 60% 167 1 67 1,6 10 1, E N Sul Concluise Conclui se (P (AM 2 41 4, 08 0, 6 16

Resumen Se sintetizaron una serie de catalizadores de paladio (0.5, 1.5 y 3.0% en peso), mediante impregnación a volumen de poro en un tamiz molecular mesoporoso a base de sílice (SBA-15). Todos los materiales se caracterizaron por Fisisorción de N2, DRX, MEB y HR-TEM. La actividad catalítica se evaluó en la oxidación catalítica vía húmeda (OCVH) del fenol a 160°C, 10 bar, 200 mg de catalizador y 200 mL de fenol de una solución de 500 ppm. Los resultados de la caracterización muestran una gran influencia del contenido metálico de Pd; el área específica disminuye al introducir un mayor porcentaje de paladio. Los materiales presentaron textura, estructura y morfología acorde a lo reportado en la literatura. Se encontró que el tamaño de las especies de óxido de paladio soportadas, formadas durante la etapa de calcinación fue menor en el catalizador con menor contenido metálico (Pd0.5/SBA-15) y este fue el más activo en la degradación de fenol y degradó 64 % de fenol, siendo el más activo de los catalizadores.

Abstract A series of palladium catalysts (0.5, 1.5 and 3.0 wt%) were synthesized by impregnation at pore volume on a silica-based mesoporous molecular sieve (SBA-15). Materials were characterized by N2 physisorption, X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (HR-TEM). The catalytic activity was evaluated in catalytic wet air oxidation (CWAO) of phenol at 160oC, 10 bar, 200 mg of catalyst and 200 mL of phenol from a 500 ppm solution. The results of the characterization show a great influence of the metallic content of Pd; the specific area decreases when introducing a higher percentage of palladium. The materials presented texture, structure and morphology according to what has been observed in literature. It was found that the size of the supported palladium oxide species formed during the calcination stage was smaller in the catalyst with lower metal content (Pd0.5/SBA-15) and this was the most active in the degradation of phenol and degraded 64% of phenol, being the most active of the catalysts.

RESUMO Avaliou-se a produção de calor corporal de vacas F1 HxZ manejadas em diferentes microclimas. Foram avaliadas 48 vacas, divididas em três grupos, manejadas em pastos e microclimas diferentes. Cada grupo foi subdividido em três grupos de suplementação fornecida durante a ordenha. Os registros termográficos dos flancos direito e esquerdo ocorreram com os animais a pasto. As temperaturas do olho e retal foram mensuradas após a ordenha. O ITGU caracterizou sinal de perigo para o microclima 1 no período da tarde (81,7) e para o microclima 2 nos turnos da manhã e da tarde (81,6 e 83,8, respectivamente). No microclima 2, houve diferença da temperatura do flanco direito para animais do grupo 2 em relação aos animais do grupo 3. A temperatura do flanco esquerdo foi superior para os animais do grupo 1. O microclima 1 foi caracterizado de conforto térmico, e o 2 de desconforto nos períodos da manhã e da tarde. A suplementação não influenciou na produção de calor dos animais. As médias da temperatura retal e do olho não diferiram entre os grupos, e a correlação apresentou valores baixos e moderados para os microclimas 1 e 2, respectivamente. A oferta de diferentes fontes de suplementação não interfere na produção de calor corporal de vacas F1 HxZ, mesmo quando fornecido em ambiente climático em que o ITGU é próximo a 84.

ABSTRACT Body heat production of F1 HxZ cows managed in different microclimates was evaluated. We evaluated 48 cows, divided into two groups, managed in different pastures and microclimates. Each group was subdivided into three groups of supplementation provided during milking. The thermographic records of the right and left flanks occurred with the animals on the grass. Eye and rectal temperatures were measured after milking. The BGT had a danger signal for microclimate 1 in the afternoon (81.7) and microclimate 2 in the morning and afternoon shifts (81.6 and 83.8, respectively). In microclimate 2, there was difference in the temperature of the right flank for animals in group 2 in relation to the animals in group 3. The temperature of the left flank was higher for the animals in the group 1. Microclimate 1 was characterized by thermal comfort and 2 was discomfort in the morning and afternoon. Supplementation did not influence the heat production of the animals. Rectal and eye mean averages did not differ between groups and the correlation presented low and moderate values for microclimate 1 and 2, respectively. The supply of different sources of supplementation does not interfere with the production and body heat of F1 HxZ cows even when supplied in a climatic environment in which the BGT is close to 84.

ABSTRACT The organic extracts from stems, roots and leaves of Tephrosia egregia Sandwith, Fabaceae, provided a new flavone, 5-hydroxy-8-(1",2"-epoxy-3"-hydroxy-3"-methylbutyl)-7-methoxyflavone (1), in addition to eleven known compounds: pongaflavone (2), praecansone B (3), 12a-hydroxyrotenone (4), praecansone A, 2',6'-dimethoxy-4',5'-(2",2"-dimethyl)-pyranochalcone, pongachalcone, maackiain, β-sistosterol and its glucoside, p-cumaric acid and cinnamic acid. The structures of all compounds were established on the basis of spectroscopic methods, mainly 1D and 2D NMR and HRESIMS, involving comparison with literature data. Cytotoxicity of compounds 1–4 was evaluated against AGP-01 (cancerous ascitic fluid), HCT-116 (colon adenocarcinoma), HL-60 (leukemia), PC-3 (prostate carcinoma), SF-295 (glioblastoma) and SKMEL 28 (melanoma) cell lines.

Avaliou-se o crescimento, a produção e o teor do óleo essencial de dois cortes de capim citronela em cultivo consorciado com algodoeiro colorido no semiárido. Para isso foram utilizados dois sistemas de consórcios (tratamentos): algodão colorido consorciado com capim citronela 3x1 e algodão colorido consorciado com capim citronela 1x1. A variedade de algodão colorido utilizada foi a BRS Rubi e as mudas de capim citronela foram produzidas no Horto de Plantas Medicinais da Unimontes. O delineamento experimental utilizado foi em blocos casualizados com dois tratamentos e 16 repetições. O experimento foi mantido em regime de sequeiro e foi avaliado a altura das plantas de capim citronela. Foram realizadas duas colheitas das folhas de capim citronela, sendo a primeira no momento da colheita da fibra do algodão e a segunda na rebrota do capim, seis meses após. Todas as plantas da parcela foram colhidas e as folhas frescas foram pesadas, no campo, com o auxílio de balança digital. Amostras das folhas colhidas foram retiradas e levadas para secagem em estufa com circulação forçada de ar a 35ºC até atingirem massa constante. Foi verificada a massa seca e posteriormente realizada a extração do óleo essencial pelo método de hidrodestilação em aparelho modificado de Clevenger. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste Skott-Knott (p<0,05). A altura das plantas não diferiu entre os tratamentos nas colheitas. Para a produção de massa fresca e seca houve diferença entre os tratamentos apenas na segunda colheita. Neste caso, as plantas cultivadas em consórcio 1x1 produziram mais do que as do consórcio 3x1. O teor de óleo essencial de capim citronela não variou entre os dois sistemas de consórcio, tanto no primeiro como no segundo corte.

The research was performed to evaluate the growth, production and essential oil content of the two harvests of citronella grass in intercropped with colored cotton in semiarid.For this, was used two system consortium (treatments): colored cotton intercropped with citronella grass 3X1 and colored cotton intercropped with citronella grass 1X1. The variety of colored cotton used was BRS Rubi and the citronella grass seedlings were produced in the Medicinal Plants Garden of Unimontes. The experimental design used was randomized blocks with two treatments and 16 repetitions. The experiment was maintained under rainfed conditions. Was evaluated plant height of citronella grass. Citronella grass leaves were harvested twice: the first was made at harvest of cotton fiber and the second in the grass regrowth, six months later. All plants in the plot were harvested and the fresh leaves were weighed, in the field, with the aid of a digital balance. Samples from leaves harvested were collected and taken for drying in an oven with forced air at 35ºC until reaching constant weight. Dry mass was verified and the performed the extraction of essential oil by hydrodestilation in Clevenger modified apparatus. Data were subjected to analysis of variance and means compared by Skott-Knott test (p<0.05). The plants height did not differ between treatments in harvests. For the production of fresh and dry mass was significant difference between treatments only in the second harvest. In this case the plants cultivated in the consortium 1x1 produced more than in the consortium 3x1. The essential oil content of citronella grass did not vary between the two systems consortium, in the first and the second harvest.

Shigella spp are Gram-negative, anaerobic facultative, non-motile, and non-sporulated bacilli of the Enterobacteriaceae family responsible for "Shigellosis" or bacillary dysentery, an important cause of worldwide morbidity and mortality. However, despite this, there are very few epidemiological studies about this bacterium in Brazil. We studied the antibiotic resistance profiles and the clonal structure of 60 Shigella strains (30 S. flexneri and 30 S. sonnei) isolated from shigellosis cases in different cities within the metropolitan area of Campinas, State of São Paulo, Brazil. We used the following well-characterized molecular techniques: enterobacterial repetitive intergenic consensus, repetitive extragenic palindromic, and double-repetitive element-polymerase chain reaction to characterize the bacteria. Also, the antibiotic resistance of the strains was determined by the diffusion disk method. Many strains of S. flexneri and S. sonnei were found to be multi-resistant. S. flexneri strains were resistant to ampicillin in 83.3% of cases, chloramphenicol in 70.0%, streptomycin in 86.7%, sulfamethoxazole in 80.0%, and tetracycline in 80.0%, while a smaller number of strains were resistant to cephalothin (3.3%) and sulfazotrim (10.0%). S. sonnei strains were mainly resistant to sulfamethoxazole (100.0%) and tetracycline (96.7%) and, to a lesser extent, to ampicillin (6.7%) and streptomycin (26.7%). Polymerase chain reaction-based typing supported the existence of specific clones responsible for the shigellosis cases in the different cities and there was evidence of transmission between cities. This clonal structure would probably be the result of selection for virulence and resistance phenotypes. These data indicate that the human sanitary conditions of the cities investigated should be improved.