Resumen Objetivo Evaluar el nivel de incapacidad funcional e identificar los factores asociados en las personas después de un accidente cerebrovascular isquémico. Métodos Cohorte prospectiva, realizada en un hospital de referencia en neurología, con 224 personas con accidente cerebrovascular isquémico. La recopilación de datos se llevó a cabo entre marzo y octubre de 2019. Se acompañó a los participantes durante la internación, momento en que se recopilaron las variables sociodemográficas y clínicas mediante la utilización de instrumentos específicos, y se los contactó 90 días después, por teléfono, para aplicar el Índice de Barthel modificado. En el análisis se aplicó estadística descriptiva y la prueba χ2 de Pearson. Se adoptó significación estadística de 5 %. Resultados La mayoría presentó algún nivel de incapacidad funcional (58,5 %), de los cuales el 29,5 % presentó dependencia moderada y el 29,0 % dependencia severa o grave. Las siguientes variables fueron asociadas a un mayor nivel de incapacidad: sexo femenino (p=0,011), tiempo de llegada al hospital de referencia mayor o igual a 4,5 h (p=0,017), accidente cerebrovascular previo (p=0,031), no haber realizado trombólisis (p=0,023), tener hipertensión arterial (p=0,032) y mayor gravedad estimada por la National Institute of Health Stroke Scale (p=0,000). Conclusión Predominó la dependencia de moderada a grave. La gravedad del evento, evento previo, hipertensión, no realización de trombólisis, retraso de la llegada al hospital y sexo femenino fueron las variables asociadas a un mayor nivel de incapacidad funcional. isquémico prospectiva neurología 22 2019 internación específicos 9 teléfono modificado χ Pearson 58,5 585 58 (58, %, , %) 295 29 29, 290 0 grave p=0,011, p0011 p p=0,011 011 (p=0,011) 45 4 4, p=0,017, p0017 p=0,017 017 (p=0,017) p=0,031, p0031 p=0,031 031 (p=0,031) p=0,023, p0023 p=0,023 023 (p=0,023) p=0,032 p0032 032 (p=0,032 p=0,000. p0000 p=0,000 . 000 (p=0,000) 2 201 58, (58 p001 p=0,01 01 (p=0,011 (p=0,017 p003 p=0,03 03 (p=0,031 p002 p=0,02 02 (p=0,023 (p=0,03 p000 p=0,00 00 (p=0,000 20 (5 p00 p=0,0 (p=0,01 (p=0,02 (p=0,0 (p=0,00 ( p0 p=0, (p=0, p=0 (p=0 p= (p= (p

Abstract Objective To assess the functional disability level and identify associated factors in people after Ischemic Cerebral Vascular Accident. Methods A prospective cohort, carried out in a reference hospital in neurology with 224 people with ischemic stroke. Data collection took place between March and October 2019. Participants were followed up during hospitalization, when sociodemographic and clinical variables were collected using specific instruments and contacted after 90 days, by telephone call, to apply the Modified Barthel Index. In the analysis, descriptive statistics and Pearson’s chi-square test were applied. Statistical significance of 5% was adopted. Results Most had some degree of functional disability (58.5%), with 29.5% having moderate dependence and 29.0% having severe dependence. The variables being female (p=0.011), time of arrival at the reference hospital greater than or equal to 4.5 hours (p=0.017), previous stroke (p=0.031), not having undergone thrombolysis (p=0.023), having high blood pressure (p=0.032) and greater severity estimated by the National Institute of Health Stroke Scale (p=0.000) were associated with a higher disability level. Conclusion Moderate to severe dependence predominated. The severity of the event, previous event, hypertension, non-submission to thrombolysis, delay in arriving at the hospital and female gender were associated with a higher functional disability level. Accident cohort 22 2019 hospitalization 9 days call Index analysis Pearsons Pearson s chisquare chi square applied 5 adopted 58.5%, 585 58.5% , 58 (58.5%) 295 29 29.5 290 0 29.0 p=0.011, p0011 p p=0.011 011 (p=0.011) 45 4 4. p=0.017, p0017 p=0.017 017 (p=0.017) p=0.031, p0031 p=0.031 031 (p=0.031) p=0.023, p0023 p=0.023 023 (p=0.023) p=0.032 p0032 032 (p=0.032 p=0.000 p0000 000 (p=0.000 predominated event hypertension nonsubmission non submission 2 201 58.5 (58.5% 29. p001 p=0.01 01 (p=0.011 (p=0.017 p003 p=0.03 03 (p=0.031 p002 p=0.02 02 (p=0.023 (p=0.03 p=0.00 p000 00 (p=0.00 20 58. (58.5 p00 p=0.0 (p=0.01 (p=0.02 (p=0.0 (58. p0 p=0. (p=0. (58 p=0 (p=0 (5 p= (p= ( (p

Resumo Objetivo Avaliar o nível de incapacidade funcional e identificar os fatores associados em pessoas após Acidente Vascular Cerebral Isquêmico. Métodos Coorte prospectiva, realizada em hospital de referência em neurologia, com 224 pessoas com acidente vascular cerebral isquêmico. A coleta de dados ocorreu entre março a outubro de 2019. Os participantes foram acompanhados durante a internação, quando as variáveis sociodemográficas e clínicas foram levantadas empregando-se instrumentos específicos e contatados após 90 dias, por ligação telefônica, para aplicação do Índice de Barthel modificado. Na análise, aplicou-se estatística descritiva e o teste Qui-quadrado de Pearson. Adotou-se significância estatística de 5%. Resultados A maioria apresentou algum grau de incapacidade funcional (58,5%), sendo que 29,5% apresentaram dependência moderada e 29,0% severa ou grave. As variáveis sexo feminino (p=0,011), tempo de chegada ao hospital de referência maior ou igual a 4,5h (p=0,017), Acidente vascular cerebral prévio (p=0,031), não ter realizado trombólise (p=0,023), ter hipertensão arterial (p=0,032) e maior gravidade estimada pela National Institute of Health Stroke Scale (p=0,000) foram associadas a maior nível de incapacidade. Conclusão Predominou a dependência de moderada a grave. A gravidade do evento, evento prévio, hipertensão, não submissão à trombólise, retardo à chegada ao hospital e sexo feminino foram associados a maior nível de incapacidade funcional. Isquêmico prospectiva neurologia 22 isquêmico 2019 internação empregandose empregando se 9 dias telefônica modificado análise aplicouse aplicou Quiquadrado Qui quadrado Pearson Adotouse Adotou 5 5% 58,5%, 585 58,5% , 58 (58,5%) 295 29 29,5 290 0 29,0 grave p=0,011, p0011 p p=0,011 011 (p=0,011) 45h h 4 5h p=0,017, p0017 p=0,017 017 (p=0,017) p=0,031, p0031 p=0,031 031 (p=0,031) p=0,023, p0023 p=0,023 023 (p=0,023) p=0,032 p0032 032 (p=0,032 p=0,000 p0000 000 (p=0,000 2 201 58,5 (58,5% 29, p001 p=0,01 01 (p=0,011 (p=0,017 p003 p=0,03 03 (p=0,031 p002 p=0,02 02 (p=0,023 (p=0,03 p=0,00 p000 00 (p=0,00 20 58, (58,5 p00 p=0,0 (p=0,01 (p=0,02 (p=0,0 (58, p0 p=0, (p=0, (58 p=0 (p=0 (5 p= (p= ( (p

Abstract Introduction: Scientific research indicates that spirituality plays an important role in the daily life of most patients. Moreover, there are studies indicating that religious activities and beliefs may be related to better health and quality of life1. Objective: to evaluate the level of spirituality of medical students and graduated physicians, in addition to analyzing the teaching of the “Medicine and Spirituality” interface in medical school. Methodology: A descriptive cross-sectional study was carried out by applying questionnaires to medical students and doctors at a Brazilian public medical school. Results: a total of 234 participants were evaluated. Most believe in a higher power. Spirituality was higher among doctors who had already graduated and among females. Most believe that university education does not prepare doctors to address the topic with the patients. Despite this limitation, most have already discussed spirituality with their patients. Conclusion: Doctors and medical students consider it important to contemplate spiritual aspects of patients using an ecumenical and respectful approach. Nevertheless, they consider they were not sufficiently prepared in medical school for this approach. Introduction Moreover life1 Objective physicians Medicine Methodology crosssectional cross sectional Results 23 evaluated power females limitation Conclusion approach Nevertheless 2

Resumo Introdução: Pesquisas científicas indicam que a espiritualidade desempenha um papel importante na vida da maioria dos pacientes. Além disso, atividades e crenças religiosas podem, de acordo com algumas pesquisa, estar relacionadas à melhor saúde e qualidade de vida1. Objetivo: Este estudo teve como objetivos avaliar o nível de espiritualidade de estudantes de Medicina e de médicos já formados, e analisar o ensino da interface “medicina e espiritualidade” na escola médica. Método: Realizamos um estudo transversal descritivo por meio da aplicação de questionários a estudantes de Medicina e médicos de uma escola médica pública brasileira. Resultado: Avaliaram-se 234 participantes. A maioria acredita em uma força superior. A espiritualidade foi maior entre médicos já formados e entre pessoas do sexo feminino. A maioria acredita que a formação universitária não prepara o médico para abordar o tema com os pacientes. Apesar dessa limitação, a maioria já abordou a espiritualidade com seus pacientes. Conclusão: Médicos e estudantes de Medicina consideram importante contemplar, de maneira ecumênica e respeitosa, aspectos espirituais dos pacientes. Apesar disso, consideram que não receberam preparo suficiente na escola médica para essa abordagem. Introdução pacientes disso podem pesquisa vida1 Objetivo medicina Método brasileira Resultado Avaliaramse Avaliaram se 23 participantes superior feminino limitação Conclusão contemplar respeitosa abordagem 2

SUMMARY OBJECTIVE: The aim of this study was to analyze possible alterations (morphological and inflammatory) in the ocular cells of fetuses from mothers with insulin resistance exposed to saturated fatty acids through the period of pregnancy. METHODS: Wistar female rats were induced to develop insulin resistance before pregnancy. Fetuses' skulls were collected on the 20th day of intrauterine life. The rats were separated on the first day of management into two groups according to the diet applied: control group (C): diet containing soybean oil as a source of fat; and saturated fatty acid group (S): diet containing butter as a source of fat. RESULTS: Histological and immunohistochemical analyses have been conducted. The immunohistochemical analyses of interleukin 6, suppressor of cytokine signaling, 3 and signal transducer and activator of transcription 3 did not demonstrate alterations in the expression of proteins in the fetuses of mothers fed with a saturated fatty diet. Moreover, no histopathological changes were noticed between groups. CONCLUSION: The saturated fatty diet does not induce tissue changes or activate the Janus kinase/signal transducer and activator of transcription signaling pathway during eye development in the fetuses of mothers with insulin resistance. OBJECTIVE morphological inflammatory pregnancy METHODS Fetuses th life applied C (C) fat S (S) RESULTS conducted 6 Moreover CONCLUSION kinasesignal kinase (C (S

Resumo A qualidade espermática é parâmetro fundamental para a reprodução eficaz de peixes em cativeiro e para o desenvolvimento de técnicas reprodutivas, como a criopreservação de sêmen. Este estudo teve como objetivo determinar a composição do plasma seminal de Pseudoplatystoma reticulatum e analisar as relações entre os componentes do plasma seminal e as características espermáticas. Nove machos foram induzidos à espermiação com extrato de hipófise de carpa no período reprodutivo da espécie (novembro e dezembro/2019). As características do sêmen foram avaliadas: motilidade espermática subjetiva, motilidade, duração, volume espermático liberado, concentração espermática, pH, osmolalidade e composição do plasma seminal, incluindo níveis de cálcio, cloreto, sódio, magnésio, potássio, glicose, frutosamina, triglicerídeos e proteína total. Para determinar a relação entre os componentes do plasma seminal e os parâmetros de motilidade espermática, foi realizada uma análise de componentes principais (PCA). O plasma seminal de P. reticulatum é composto principalmente pelo íon Na+ e componentes orgânicos como proteínas e glicose. Através da PCA, observou-se que a motilidade espermática apresentou forte correlação positiva com tempo de motilidade, concentração espermática e proteína total e correlação negativa com osmolalidade e frutosamina. reprodutivas espermáticas novembro dezembro/2019. dezembro2019 dezembro dezembro/2019 . 2019 dezembro/2019) avaliadas subjetiva duração liberado pH cálcio cloreto sódio magnésio potássio glicose frutosamina PCA. PCA (PCA) P Na observouse observou se dezembro201 dezembro/201 201 (PCA dezembro20 dezembro/20 20 dezembro2 dezembro/2 2 dezembro/

Abstract Sperm quality is a fundamental parameter for the effective reproduction of fish in captivity and the development of reproductive techniques, such as semen cryopreservation. This study aimed to determine the composition of the seminal plasma of Pseudoplatystoma reticulatum and analyze the relationships between plasma components and sperm characteristics. Nine males were induced to spermiation with carp pituitary extract in the reproductive period of the species (November and December/2019). Semen characteristics were evaluated: subjective sperm motility, motility, duration, released sperm volume, sperm concentration, pH, osmolality, and seminal plasma composition, including levels of calcium, chloride, sodium, magnesium, potassium, glucose, fructosamine, triglycerides, and total protein. To determine the relationship between seminal plasma components and sperm motility parameters, a principal component analysis (PCA) was performed. The seminal plasma of P. reticulatum is composed mainly of the Na+ ion and organic components such as protein and glucose. Through PCA, it was observed that sperm motility had a strong positive correlation with motility time, sperm concentration, and total protein and a negative correlation with osmolality and fructosamine. techniques cryopreservation November December/2019. December2019 December December/2019 . 2019 December/2019) evaluated duration volume concentration pH calcium chloride sodium magnesium potassium glucose fructosamine triglycerides parameters PCA (PCA performed P Na time December201 December/201 201 December20 December/20 20 December2 December/2 2 December/

ABSTRACT Toxoplasmosis is an infection of vast worldwide distribution whose etiologic agent is Toxoplasma gondii. This disease can cause problems ranging from mild symptoms to serious conditions, such as encephalitis, miscarriage and blindness. Therefore, it is of utmost importance to perform a diagnosis with reproducible techniques in order to obtain a good prognosis. The aim of this review was to analyze the efficiency of toxoplasmosis diagnostic techniques based on sensitivity and specificity results. Five research platforms in English language were used (Eric, Elsevier, Google Scholar, PubMed and SciELO), which contained data on the diagnosis of toxoplasmosis. The search and selection were performed for studies published prior to June 2021. The search resulted in the inclusion of 13 articles published from 2005 to 2020. The data revealed the use of different samples in the standardization of techniques such as serum, total blood, colostrum and amniotic fluid. The flow cytometry, lateral flow immunoassay and qPCR techniques showed 100% sensitivity, whereas the ELISA, western blotting, qPCR and RE-LAMP techniques achieved 100% specificity. Significantly, the qPCR and LAMP techniques were more accurate when the likelihood ratio was assessed. The meta-analysis identified that ISAGA and western blotting have low sensitivity values and LIASON, ELFA and ELISA, using a silica bioconjugate, also have low specificity values. It was noted that a wide range of methods have high values of sensitivity and specificity. Therefore, the choice of the method will be based on the conditions and its financial viability. gondii encephalitis blindness Therefore prognosis results Eric, Eric (Eric Elsevier Scholar SciELO, SciELO , SciELO) 2021 1 200 2020 serum blood fluid cytometry 100 ELISA RELAMP RE Significantly assessed metaanalysis meta analysis LIASON bioconjugate viability 202 20 10 2

Abstract The frequency of gastrointestinal parasites with an emphasis on Strongylus vulgaris was investigated among the Brazilian Pony breed kept on farms in the municipality of Teresópolis, state of Rio de Janeiro. Fecal samples were collected in three stud farms: A (n= 22 animals), B (n= 3), and C (n= 2). Fecal samples were subjected to the quantitative Mini-FLOTAC technique, using three different solutions, and to qualitative techniques. The parasite prevalence was found to be 81.4%. Eggs from strongylids were identified in 74% of the ponies. Eggs of Parascaris spp. were detected in 22.7% of the animals, which were all females of farm A. At this locality, mares were kept with their foals in fenced paddocks all the time. The NaCl solution of d = 1.200 g/ml was generally the one that presented the highest frequency of diagnosis of nematode eggs and the highest mean of fecal eggs per gram. The fecal samples were also subjected to the polymerase chain reaction for amplification of DNA from the ITS2 region for Strongylus vulgaris. Twelve samples presented nucleotide sequences for S. vulgaris. In the end, this study revealed the high frequency (96.3%) of S. vulgaris among ponies on farms in Teresópolis, Rio de Janeiro, Brazil. Teresópolis Janeiro n= n (n 2 animals , animals) 3, 3 3) 2. . 2) MiniFLOTAC Mini FLOTAC technique solutions techniques 814 81 4 81.4% 74 spp 227 7 22.7 locality time 1200 1 200 1.20 gml g ml gram ITS S end 96.3% 963 96 (96.3% Brazil 8 81.4 22. 120 20 1.2 96.3 9 (96.3 81. 12 1. 96. (96. (96 (9 (

Resumo A frequência de parasitos gastrointestinais, com ênfase na pesquisa de Strongylus vulgaris, foi investigada entre os Pôneis Brasileiros criados em haras na cidade de Teresópolis, no estado do Rio de Janeiro. Amostras fecais foram coletadas em três haras: A (n=22 animais), B (n=3) e C (n=2). Amostras fecais foram submetidas à técnica quantitativa de Mini-FLOTAC utilizando três diferentes soluções e técnicas qualitativas. A prevalência de parasitos foi de 81,4%. Ovos de estrôngilos foram identificados em 74% dos pôneis. Ovos de Parascaris spp. foram detectados em 22,7% dos animais, sendo todos fêmeas do haras A. Nesta propriedade, as éguas eram mantidas com os pôneis em piquetes cercados durante todo o tempo. A solução de NaCl, com densidade de 1.200 g/ml, foi a que apresentou a maior frequência diagnóstica de ovos de nematoides e a maior contagem de ovos por grama de fezes. As amostras também foram submetidas à reação de polimerase em cadeia para amplificar DNA da região ITS2 de Strongylus vulgaris. Doze amostras fecais apresentaram sequências nucleotídicas de S. vulgaris. Ao final, este estudo demonstrou a alta frequência (96.3%) de S. vulgaris em pôneis mantidos em haras na cidade de Teresópolis, Rio de Janeiro, Brazil. gastrointestinais Teresópolis Janeiro n=22 n22 n 22 (n=2 animais , animais) n=3 n3 3 (n=3 n=2. n2 n=2 . 2 (n=2) MiniFLOTAC Mini FLOTAC qualitativas 814 81 4 81,4% 74 spp 227 7 22,7 propriedade tempo NaCl 1200 1 200 1.20 gml g ml g/ml fezes ITS S final 96.3% 963 96 (96.3% Brazil (n= n= 8 81,4 22, 120 20 1.2 96.3 9 (96.3 (n 81, 12 1. 96. (96. (96 (9 (

RESUMEN Objetivo: Verificar la asociación entre factores sociodemográficos, clínicos, ambientales, cognitivos, y emocionales y el tiempo de decisión de las personas con accidente cerebrovascular isquémico para buscar un servicio de salud luego del inicio de los síntomas o Wake up stroke Método: Estudio transversal realizado de marzo a octubre de 2019, con 304 pacientes, en un hospital público, referencia en neurología. Datos obtenidos por entrevista y de la historia clínica. El tiempo de decisión se analizó como una media geométrica. En los análisis bivariados y multivariados se utilizó la regresión lineal y el Criterio de Información de Akaike para seleccionar el mejor modelo. Se adoptó una significancia estadística del 5% Resultados: La media geométrica del tiempo de decisión fue de 0,30h (IC 95% 0,23-0,39). El modelo final explicó este tiempo en un 41%, mostrando un aumento de 0,5 min para las personas con hipertensión arterial, 10,8 min para las que esperaron a que los síntomas mejoraran, 1,4 min para las que estaban solas al inicio de los síntomas, 3, 9 min para las que estaban en casa, 3,2 min para los que estaban en el trabajo y 2,1 en la calle/vía pública Conclusión: El tiempo promedio de decisión para buscar un servicio de salud fue alto e influenciado por variables clínicas, ambientales, cognitivas y emocionales. Los resultados orientan a los enfermeros hacia la educación en salud. Objetivo sociodemográficos clínicos ambientales cognitivos Método 2019 30 pacientes público neurología clínica 5 Resultados 030h h 0 30h IC 95 0,230,39. 023039 0,23 0,39 . 23 39 0,23-0,39) 41 41% 05 0, arterial 108 10 8 10, mejoraran 14 1 4 1, 3 casa 32 2 21 2, callevía calle vía Conclusión clínicas 201 230 0,230,39 02303 023 0,2 039 0,3 0,23-0,39 20 0,230,3 0230 02 03 0,23-0,3 0,230, 0,23-0, 0,230 0,23-0 0,23-

ABSTRACT Objective: To verify the association between sociodemographic, clinical, environmental, cognitive, and emotional factors and the decision time of people with ischemic stroke to seek a health service after the onset of symptoms or wake up stroke. Method: Cross-sectional study carried out from March to October 2019, with 304 patients, in a public hospital, a reference in neurology. Data obtained through interview and from medical records. Decision time was analyzed as a geometric mean. In the bivariate and multivariate analyses, linear regression was used and the Akaike Information Criterion was used to select the best model. Statistical significance of 5% was adopted. Results: The geometric mean of decision time was 0.30h (95% CI 0.23–0.39). The final model explained this time in 41%, showing an increase of 0.5 min for people with arterial hypertension; 10.8 min for those who waited for symptoms to improve; 1.4 min for those who were alone at the onset of symptoms; 3.9 min for those at home; 3.2 min for the ones at work; and 2.1 for those on the street/public space. Conclusion: The mean decision time for seeking a health service was high and influenced by clinical, environmental, cognitive, and emotional variables. The results guide nurses regarding health education. Objective sociodemographic clinical environmental cognitive Method Crosssectional Cross sectional 2019 30 patients hospital neurology records analyses 5 adopted Results 030h h 0 30h 95% 95 (95 0.23–0.39. 023039 0.23–0.39 . 23 39 0.23–0.39) 41 41% 05 0. hypertension 108 10 8 10. improve 14 1 4 1. 3 9 3. home 32 2 work 21 2. streetpublic street space Conclusion variables education 201 (9 02303 0.23–0.3 20 ( 0230 0.23–0. 023 0.23–0 02 0.23– 0.23 0.2

RESUMO Objetivo: Verificar a associação entre fatores sociodemográficos, clínicos, ambientais, cognitivos e emocionais e o tempo de decisão de pessoas com acidente vascular cerebral isquêmico para procurar um serviço de saúde após o início dos sintomas ou Wake up stroke Método: Estudo transversal realizado de março a outubro de 2019, com 304 pacientes, em hospital público, referência em neurologia. Dados obtidos por entrevista e no prontuário. O tempo de decisão foi analisado em média geométrica. Nas análises bivariadas e multivariada foi usada a regressão linear e o Critério de Informação de Akaike foi usado na seleção do melhor modelo. Adotou- se significância estatística de 5% Resultados: A média geométrica do tempo de decisão foi de 0,30h (IC 95% 0,23-0,39). O modelo final explicou em 41% esse tempo, mostrando aumento de 0,5 min para pessoas com hipertensão arterial; 10,8 min para quem esperou os sintomas melhorarem; 1,4 min para quem estava sozinho no início dos sintomas; 3,9 min para quem estava em casa; 3,2 min no trabalho e 2,1 na rua/via pública Conclusão: A média do tempo de decisão para procura de serviço de saúde foi alta e influenciada por variáveis clínicas, ambientais, cognitivas e emocionais. Os resultados orientam enfermeiros para a educação em saúde. Objetivo sociodemográficos clínicos ambientais Método 2019 30 pacientes público neurologia prontuário Adotou 5 Resultados 030h h 0 30h IC 95 0,230,39. 023039 0,23 0,39 . 23 39 0,23-0,39) 41 05 0, arterial 108 10 8 10, melhorarem 14 1 4 1, 3 9 3, casa 32 2 21 2, ruavia rua via Conclusão clínicas 201 230 0,230,39 02303 023 0,2 039 0,3 0,23-0,39 20 0,230,3 0230 02 03 0,23-0,3 0,230, 0,23-0, 0,230 0,23-0 0,23-

Abstract This study aimed to verify whether the inclusion of omega-3 polyunsaturated fatty acids (PUFAs-ô3) in the diet of Astyanax lacustris males improves seminal quality. Four hundred fish were arranged in 20 boxes of 180 L in the recirculation system. These were fed with isoprotein diets (32% CP), until apparent satiation, with four levels of marine fish oil rich in PUFAs-ô3 inclusion (In% = 0, 3, 6, and 9), for 105 days. After, males were hormonally induced to spermiation, and semen was collected after 226 ATUs. Parameters evaluated were osmolality, seminal volume and color, spermatic concentration, sperm morphology, membrane integrity and, sperm motility (total and progressive motility, velocity curvilinear, straight line and average path, linearity and rectilinearity coefficients, trajectory oscillation, head lateral displacement amplitude, and cross-beat frequency). Seminal volume was greater in the In-0 and In-3 groups. Inclusion of PUFAs-ô3 positively influenced the kinetic parameters, as treatment with 6% and 9% of inclusion fish oil resulted in higher values for most of these parameters and did not differ statistically from each other. Thus, it is concluded that the addition of PUFAs-ô3 to the feed of breeders significantly improved the seminal quality of A. lacustris males. omega3 omega 3 omega- PUFAsô3 PUFAsô PUFAs ô3 ô (PUFAs-ô3 2 18 system 32% 32 (32 CP, CP , CP) satiation PUFAs-ô In% In (In 0 6 9, 9 9) 10 days After spermiation 22 ATUs osmolality color concentration morphology total curvilinear path coefficients oscillation amplitude crossbeat cross beat frequency. frequency . frequency) In0 In- In3 groups other Thus A (PUFAs-ô 1 (3 (

Resumo Este estudo teve como objetivo verificar se a inclusão de ácidos graxos poliinsaturados ômega-3 (PUFAs-ô3) na dieta de machos de Astyanax lacustris melhora a qualidade seminal. Quatrocentos peixes foram dispostos em 20 caixas de 180 L no sistema de recirculação. Estes foram alimentados com dietas isoproteicas (32% PB) até aparente saciedade, com quatro níveis de óleo de peixe marinho rico em inclusão de PUFAs-ô3 (In% = 0, 3, 6 e 9), durante 105 dias. Após, os machos foram induzidos hormonalmente à espermiação e o sêmen foi coletado após 226 UTAs. Os parâmetros avaliados foram osmolalidade volume e cor seminal concentração espermática morfologia espermática integridade de membrana e motilidade espermática (motilidade total e progressiva velocidade curvilínea linha reta e trajeto médio coeficientes de linearidade e retilinearidade, oscilação da trajetória, amplitude de deslocamento lateral da cabeça e frequência de batida cruzada). O volume seminal foi maior nos grupos In-0 e In-3. A inclusão de PUFAs-ô3 influenciou positivamente os parâmetros cinéticos, pois o tratamento com 6% e 9% de inclusão de óleo de peixe resultou em valores mais elevados para a maioria desses parâmetros e não diferiram estatisticamente entre si. Assim, conclui-se que a adição de PUFAs-ô3 na ração dos criadores melhorou significativamente a qualidade seminal dos machos de A. lacustris. ômega3 ômega 3 ômega- PUFAsô3 PUFAsô PUFAs ô3 ô (PUFAs-ô3 2 18 recirculação 32% 32 (32 PB saciedade PUFAs-ô In% In (In 0 9, 9 , 9) 10 dias Após 22 UTAs retilinearidade trajetória cruzada. cruzada . cruzada) In0 In- In3. In3 3. In-3 cinéticos si Assim concluise conclui (PUFAs-ô 1 (3 (

Abstract Background Lacosamide (LCM) is a third-generation anti-seizure drug approved in Europe and the United States, either as a monotherapy or adjunctive therapy, to treat partial-onset seizures in adults, adolescents, and children. In Brazil, LCM is licensed for treatment only in patients older than 16 years of age. Objective To evaluate a cohort of children presenting with refractory epilepsy who received LCM as an add-on therapy and observe the response and tolerability to the LCM treatment. Methods A retrospective cohort study conducted in a tertiary health care facility, which included 26 children, aged up to 16 years, who presented with refractory epilepsy and received LCM as an add-on treatment. The follow-up visits were scheduled every 3 months until 9 months of treatment with LCM. Results After 3 months of LCM administration, in 73.1% of the children, there was a reduction of > 50% in the frequency of seizures, and this clinical improvement was maintained in most patients (73.9%) for the following 9 months. Mild (such as, somnolence and behavioral changes) or severe (seizure worsening) adverse effects were observed in two and three children respectively. Among responders to LCM, there was a higher prevalence of males, fewer concomitant anti-seizure drugs, and lower percentage of patients using sodium channel blockers. Conclusions Lacosamide should be considered as an early treatment option in pediatric patients with refractory epilepsy, mainly focal seizures. (LCM thirdgeneration third generation antiseizure anti seizure States partialonset partial onset adults adolescents Brazil 1 age addon add on facility 2 followup follow administration 731 73 73.1 50 73.9% 739 (73.9% such changes worsening respectively males drugs blockers 7 73. 5 73.9 (73.9 (73. (73 (7 (

Resumo Antecedentes Lacosamida (LCM) é um fármaco anticrise de terceira geração aprovado na Europa e nos Estados Unidos, utilizado como monoterapia ou terapia adjuvante para tratar crises epilépticas focais em adultos, adolescentes e crianças. No Brasil, a LCM só é aprovada para tratamento em pacientes com mais de 16 anos de idade. Objetivo Avaliar uma coorte de crianças com epilepsia refratária que receberam LCM como terapia adjuvante e observar a resposta e tolerabilidade ao tratamento. Métodos Um estudo de coorte retrospectivo conduzido em uma unidade terciária de saúde, que incluiu 26 crianças de até 16 anos de idade que apresentavam epilepsia refratária e receberam um tratamento complementar com LCM. As visitas de acompanhamento foram agendadas a cada 3 meses, até 9 meses de tratamento com LCM. Resultados Após 3 meses de administração de LCM, em 73,1% das crianças, a frequência das crises teve uma redução maior do que 50%, e essa melhora clínica foi mantida na maioria dos pacientes (73,9%) pelos 9 meses seguintes. Efeitos adversos leves (como, sonolência e alterações comportamentais) ou graves (agravamento das crises) foram observados em duas e três crianças, respectivamente. Entre as crianças que responderam ao tratamento com LCM, houve uma maior prevalência do sexo masculino, o uso de um menor número de medicações anticrise associadas e o uso de bloqueadores dos canais de sódio. Conclusões A LCM deve ser considerada uma opção de tratamento precoce em pacientes pediátricos com epilepsia refratária, principalmente aqueles que apresentam crises focais. (LCM Unidos adultos Brasil 1 saúde 2 731 73 73,1 50 50% 73,9% 739 (73,9% seguintes como, (como comportamentais agravamento respectivamente masculino sódio 7 73, 5 73,9 (73,9 (73, (73 (7 (

ABSTRACT Objective: To investigate the presence of chromosome mosaicism, especially for the presence of Y derived material in 45,X women with Turner syndrome (TS). Materials and methods: FISH and PCR were performed for the presence of chromosome mosaicism and Y-derived-material and genetic findings were correlated to clinical data. Results: Thirty-one participants were enrolled: 18 (58%) had chromosome mosaicisms (FISH), Y-derived material was found in 2. Yet, SRY primer was found with PCR in only one of them and DYZ3 was not found. The most frequent clinical findings were short or webbed neck (81,82%), high-arched palate (78%), breast hypertelorism, e cubitus valgus and genu valgus (57.6%, both), short fourth metacarpals (46.9%), epicanthic folds (43.8%), shield chest (43.8%), lymphedema (37.5%), and low set ears (34.4%). Both patients with Y-derived-material had primary amenorrhea, dyslipidemia and reached the height of 150 cm despite not treated with recombinant growth hormone (GHr). One of them showed 26% of leukocytes with Y-derived material and few clinical findings. Conclusions: FISH techniques proved efficient in detecting chromosome mosaicisms and Y-derived material and searching in different tissues such as mouth cells is critical due to the possibility of tissue-specific mosaicism. Phenotypical variance in TS may be a signal of chromosome mosaicisms, especially with the presence of Y-derived material.

ABSTRACT Introduction: The mandibular buccal bifurcation cyst is a rare inflammatory odontogenic cyst that typically develops at the buccal region of the first or second permanent mandibular molars of children aged 6 to 15 years. The tooth involved is vital and shows an increased probing depth on the buccal surface. Radiographically, it is characterized by a unilocular radiolucent area, well circumscribed on the buccal osseous cortical, that it is difficult to visualize on periapical radiography. The diagnosis is primarily clinical and radiographic, and the treatment is usually the cystic enucleation without extraction of the tooth. Objective: To report a case of spontaneous involution of a unilateral mandibular buccal bifurcation cyst. Case presentation: This report describes the clinical and radiographic characteristics of an unusual case of unilateral buccal bifurcation cyst in a 6-year-old patient, presenting as a slight expansion in the buccal region of the first permanent mandibular molar. The treatment of choice was the clinical and radiographic follow-up due to the spontaneous involution of the cyst. There were no recurrences during follow-up and the tooth remained vital and erupted normally. Conclusions: The possibility of spontaneous involution is a characteristic that must always be observed, in order to avoid unnecessary surgical procedures.

RESUMEN Introducción: El quiste de bifurcación bucal mandibular es un quiste odontogénico inflamatorio poco frecuente que, en general, se desarrolla en la región bucal de primeros y segundos molares mandibulares permanentes en niños de 6-15 años. El diente involucrado es vital y muestra una mayor profundidad de sondeo en la superficie bucal. Radiográficamente, se caracteriza por un área radiolúcida unilocular, bien circunscrita en la cortical ósea bucal, que es difícil de visualizar en la radiografía periapical. El diagnóstico es principalmente clínico y radiográfico y el tratamiento suele ser la enucleación quística sin extracción del diente. Objetivo: Informar un caso de involución espontánea de un quiste de bifurcación vestibular mandibular unilateral. Presentación del caso: Caso inusual de quiste de bifurcación bucal unilateral en un paciente de 6 años, que se presenta como una ligera expansión de la región vestibular del primer molar mandibular permanente. El tratamiento de elección fue el seguimiento clínico y radiográfico debido a la involución espontánea del quiste. No hubo recurrencias durante el seguimiento y el diente permaneció vital y brotó normalmente. Conclusiones: La posibilidad de involución espontánea es una característica que siempre se debe observar para evitar procedimientos quirúrgicos innecesarios.

RESUMO Objetivo: Analisar a associação entre sexualidade e qualidade de vida de idosos brasileiros residentes em comunidade. Métodos: Estudo transversal conduzido com 477 idosos brasileiros. Os dados foram coletados entre agosto e outubro de 2020. Utilizou-se a Escala de Vivências Afetivas e Sexuais do Idoso (EVASI) e o World Health Organization Quality of Life - Old (WHOQOL-OLD). A análise dos dados foi realizada com os testes Mann-Whitney, correlação de Spearman e Kruskal-Wallis, com aplicação do post-hoc de Bonferroni quando necessário, considerando um intervalo de confiança de 95%. Resultados: Houve associação estatística entre todas as dimensões da sexualidade e a qualidade de vida geral dos idosos (p<0,05). Conclusão: O estímulo à sexualidade pode se configurar como uma estratégia inovadora e holística com foco na promoção da saúde e do envelhecimento ativo, uma vez que este estudo constatou a associação entre a sexualidade e qualidade de vida geral de idosos.

ABSTRACT Objective: to analyze the association between sexuality and quality of life of Brazilian elderly residents in the community. Methods: a cross-sectional study conducted with 477 Brazilian elderly. The data were collected between August and October 2020. We used the EVASI and WHOQOL-OLD (World Health Organization Quality of Life). Data analysis was performed with Mann-Whitney, Spearman and Kruskal-Wallis correlation tests, with Bonferroni post-hoc application when necessary, considering a 95% confidence interval. Results: there was a statistical association between all dimensions of sexuality and the general quality of life of the elderly (p<0.05). Conclusion: the stimulation of sexuality can be configured as an innovative and holistic strategy focused on the promotion of health and active aging, since this study found the association between sexuality and the general quality of life of elderly people.

RESUMEN Objetivo: Analizar la relación entre sexualidad y calidad de vida de ancianos brasileños residentes en comunidad. Métodos: Estudio transversal conducido con 477 ancianos brasileños. Los datos recogidos entre agosto y octubre de 2020. Utilizó la Escala de Vivencias Afectivas y Sexuales del Anciano (EVASI) y el World Health Organization Quality of Life - Old (WHOQOL-OLD). El análisis de los datos fue realizado con los testes Mann-Whitney, correlación de Spearman y Kruskal-Wallis, con aplicación del post-hoc de Bonferroni cuando necesario, considerando un intervalo de confianza de 95%. Resultados: Hubo relación estadística entre todas las dimensiones de la sexualidad y la calidad de vida general de los ancianos (p<0,05). Conclusión: El estímulo a la sexualidad puede configurarse como una estrategia innovadora y holística con enfoque en la promoción de la salud y del envejecimiento activo, una vez que este estudio constató la relación entre la sexualidad y calidad de vida general de ancianos.

OBJECTIVE: Considering the rapid spread of COVID-19, the scientific community has been looking for ways to recognize factors that may interfere with the outcome of viral infection. Despite the lack of studies with the new coronavirus, it is known that adequate serum levels of micronutrients are essential for the organic response to infectious diseases. Thus, we aim to review the effects of vitamin A, D, iron, zinc, or folate deficiency on the prognosis of patients with respiratory infections with manifestations similar to COVID-19 and discuss about supplementation of the nutrients analyzed in this review. METHODS: The search was conducted in the databases PubMed, Lilacs, and SciELO, including observational studies published between 2010-2020, with results for individuals with respiratory tract infections with manifestations similar to COVID-19. RESULTS: Six articles met the inclusion criteria, all of which were related to deficiencies of vitamins A and D. In general, vitamin A deficiency was associated with cough, fever, and greater total respiratory resistance. Regarding vitamin D, the lack of this nutrient led to higher rates of ICU admission, the need for mechanical ventilation, and mortality. Evidence linking specific relationships between nutritional deficiencies and COVID-19 remain lacking due to the small number of studies and heterogeneities in population subgroups. CONCLUSION: In conclusion, deficiencies of vitamins A and D seem to negatively affect the prognosis of respiratory tract infections. Supplementation of these nutrients for prevention or treatment of patients diagnosed with COVID-19 should respect serum levels, nutritional status and housing conditions (e.g.,endemic location) of individuals.

Abstract INTRODUCTION: Although supervised doses are essential for reducing leprosy treatment failure, the impact of specific drug interactions has rarely been assessed. This study aimed to estimate the risk of leprosy treatment suspension in patients receiving polypharmacy. METHODS We performed this case-control study in which the primary outcome was defined as the need to discontinue multibacillary leprosy treatment for at least one supervised dose, and the main risk factor was the detection of polypharmacy. Multivariate analysis by logistic regression was used for calculating odds ratio (OR). RESULTS: This study included 103 patients, of whom 43 needed to discontinue leprosy treatment (hemolysis = 26, hepatitis = 2, hemolysis associated with hepatitis = 6, and suspected treatment resistance = 9) and the rest did not. The severity of drug interactions had no effect on treatment discontinuation. Patients who used five or more drugs in addition to leprosy treatment had almost a 4-fold greater risk of treatment suspension (OR, 3.88; 95% confidence interval: 1.79-9.12; p < 0.001). The number of drugs used also positively influenced the occurrence of hemolysis (p < 0.001). No patient presented evidence of molecular resistance to rifampicin, dapsone, or ofloxacin treatment, as evidenced by genetic sequencing detection of rpoB, folp1, and gyrA mutations. CONCLUSIONS: Polypharmacy has deleterious effects on the already difficult-to-adhere-to treatment of leprosy and polypharmacy induces hemolysis. Additional measures must be taken to avoid the undesirable effects of inadequate polypharmacy.

Resumo Objetivo: Comparar os efeitos do Pilates vs. vibração de corpo inteiro (VCI) e nenhuma intervenção sobre o equilíbrio postural e medo de quedas em mulheres na pós-menopausa. Métodos: Ensaio clínico Mono-cego, com 51 participantes randomizadas em três grupos (Pilates, VCI, ou Controle). Avaliações foram realizadas para o equilíbrio postural estático por intermédio da plataforma de força, equilíbrio postural dinâmico pelo teste Timed Up & Go e medo de quedas pela Falls Efficacy Scale-International (FES-I). Pilates e VCI foram administrados três vezes na semana, por seis meses. Resultados: Após a intervenção, nenhuma diferença (p> 0.05) foi observada para o equilíbrio postural estático, contudo, para a maior parte das variáveis, Pilates e VCI demonstraram um grande tamanho de efeito (d> 0.80) quando comparados ao grupo controle. Para o equilíbrio postural dinâmico, Pilates e VCI promoveram uma melhora significativa (p= 0.032) comparados ao grupo controle. Para o medo de quedas não houve diferença entre os grupos (p= 0.055) pós-intervenção. Conclusão: Tendo em vista a representatividade clínica evidenciada pelas medidas de tamanho de efeito, Pilates e VCI podem ser recomendados para melhora do equilíbrio postural estático e dinâmico em mulheres na pós-menopausa. Contudo, em relação ao medo de quedas, essas modalidades terapêuticas necessitam de melhor investigação.

Abstract To compare the effects of Pilates vs. whole body vibration (WBV) and no treatment controls on postural balance and fear of falling in postmenopausal women. Method: Single-blind randomized clinical trial, with 51 participants randomized into three groups (Pilates, WBV, or control). Evaluations were performed of static postural balance on a force platform, dynamic postural balance by the Timed Up & Go test, and fear of falling by the Falls Efficacy Scale-International (FES-I). Pilates and WBV were performed three times a week for six months. Results: After the intervention, no difference (p>0.05) was observed for static postural balance, however, for the majority of variables, Pilates and WBV demonstrated a large effect size (d>0.80) when compared to control. For dynamic postural balance and risk of falls, Pilates and WBV showed a significant (p=0.032) improvement compared to the control. Fear of falling did not change (p=0.055) between groups over time. Conclusion: In view of the clinical representativeness evidenced by the effect sizes, Pilates and WBV can be recommended to improve postural balance in postmenopausal women. However, in relation to fear of falling, these therapeutic modalities require further investigation.