ABSTRACT Digital health can effectively engage patients in the self-management of their asthma. Still, it will only reach its full potential when apps are aligned with patients’ needs and are integrated into clinical practice. We aim to test a framework for the involvement of patients/carers in all phases of developing and implementing an app for asthma. This protocol was co-designed with six asthma patients/carers and includes 5 tasks. Regular interactions among patients, developers and healthcare professionals will be a priority for user requirements definition (task 1) and iterative development and testing until the app’s initial version (tasks 2-3). Patients/carers and healthcare professionals will be fully engaged in the real-life feasibility study (task 4) and management and dissemination activities (task 5). The eligibility criteria to participate in tasks 2-4 are 1) diagnosis of asthma or carer of a person with asthma; 2) ≥18 years; 3) ability to use apps and access a mobile device with Internet. We anticipate applying questionnaires such as the User Experience Questionnaire and the System Usability Scale in all app testing tasks. We expect to have a final version of the asthma app that meets patients’/carers’ needs and experiences and healthcare professionals’ concerns. This will be an attractive app to support patients in self-management of their disease, keeping their asthma in control, having fewer asthma symptoms, and enjoying life. This study will be a framework for fully involving patients, carers and citizens in the iterative co-production development and implementation of an asthma app.

RESUMO A saúde digital pode envolver efetivamente os doentes no controlo da sua doença, mas só alcançará o seu potencial quando as aplicações móveis desenvolvidas estiverem alinhadas com as necessidades percebidas pelos doentes e integradas na prática clínica. O nosso objetivo é testar uma abordagem para o envolvimento de doentes/cuidadores em todas as fases de desenvolvimento e implementação de uma aplicação móvel para a autogestão da asma. Este protocolo foi codesenvolvido com seis pessoas com asma/cuidadores e contempla cinco tarefas. Interações regulares entre doentes, investigadores, programadores e profissionais de saúde serão uma prioridade para a definição dos requisitos do utilizador (tarefa 1) e para o desenvolvimento iterativo até a versão inicial da aplicação (tarefas 2-3). Doentes/cuidadores e profissionais de saúde estarão ativamente envolvidos no estudo observacional sobre o uso da app na vida real (tarefa 4) e nas atividades de gestão/disseminação (tarefa 5). Os critérios de elegibilidade para participar nas tarefas 2-4 são: diagnóstico médico de asma ou cuidador de pessoa com este diagnóstico; pelo menos 18 anos; capacidade de usar aplicações e acesso a um dispositivo móvel com Internet. Antecipamos o uso de questionários como o Questionário de Experiência do Utilizador e a Escala de Usabilidade do Sistema em todas as tarefas que envolvem teste da aplicação. No final do estudo esperamos ter uma versão final da aplicação para a asma que atenda às necessidades e experiência dos doentes/cuidadores e às preocupações dos profissionais de saúde. Esta será uma aplicação atrativa para apoiar os doentes na autogestão da sua doença, ajudando a manter a sua asma controlada, com menos sintomas e melhor qualidade de vida. Este estudo fornecerá uma abordagem para envolver os doentes, cuidadores e cidadãos no desenvolvimento iterativo e coprodução de uma aplicação móvel de saúde para pessoas com asma.

Abstract Background: The European Society of Cardiology guidelines recommend an LDL-cholesterol (LDL-C) < 55 mg/dL for patients with established cardiovascular disease. While the Friedewald equation to estimate LDL-C is still widely used, the newer Martin-Hopkins equation has shown greater accuracy. Objectives: We aimed to assess: A) the proportion of patients reaching LDL-C goal and the therapies used in a tertiary center; B) the impact of using the Martin-Hopkins method instead of Friedewald’s on the proportion of controlled patients. Methods: A single-center cross-sectional study including consecutive post-myocardial infarction patients followed by 20 cardiologists in a tertiary hospital. Data was collected retrospectively from clinical appointments that took place after April 2022. For each patient, the LDL-C levels and attainment of goals were estimated from an ambulatory lipid profile using both Friedewald and Martin-Hopkins equations. A two-tailed p-value of < 0.05 was considered statistically significant for all tests. Results: Overall, 400 patients were included (aged 67 ± 13 years, 77% male). Using Friedewald’s equation, the median LDL-C under therapy was 64 (50-81) mg/dL, and 31% had LDL-C within goals. High-intensity statins were used in 64% of patients, 37% were on ezetimibe, and 0.5% were under PCSK9 inhibitors. Combination therapy of high-intensity statin + ezetimibe was used in 102 patients (26%). Applying the Martin-Hopkins method would reclassify a total of 31 patients (7.8%). Among those deemed controlled by Friedewald’s equation, 27 (21.6%) would have a Martin-Hopkins’ LDL-C above goals. Conclusions: Less than one-third of post-myocardial infarction patients had LDL-C within the goal. Applying the Martin-Hopkins equation would reclassify one-fifth of presumably controlled patients into the non-controlled group. Background LDLcholesterol LDL cholesterol LDLC C (LDL-C 5 mgdL mg dL disease MartinHopkins Martin Hopkins accuracy Objectives assess center B Friedewalds s Methods singlecenter single crosssectional cross sectional postmyocardial post myocardial 2 hospital 2022 patient equations twotailed two tailed pvalue p value 005 0 05 0.0 tests Results Overall 40 aged 6 1 years 77 male. male . male) 5081 50 81 (50-81 Highintensity High intensity 37 0.5 PCSK inhibitors highintensity high 10 26%. 26 26% (26%) 3 7.8%. 78 7.8% 7 8 (7.8%) 21.6% 216 21 (21.6% Conclusions onethird one third onefifth fifth noncontrolled non group 202 00 0. 4 508 (50-8 (26% 7.8 (7.8% 21.6 (21.6 (50- (26 7. (7.8 21. (21. (50 (2 (7. (21 (5 ( (7

Resumo Fundamento: As diretrizes da Sociedade Europeia de Cardiologia recomendam um nível de colesterol LDL (LDL-C) < 55 mg/dL para pacientes com doença cardiovascular estabelecida. Embora a fórmula de Friedewald ainda seja amplamente utilizada para estimar o LDL-C, a fórmula mais recente de Martin-Hopkins mostrou maior precisão. Objetivos: Nosso objetivo foi avaliar: A) a proporção de pacientes que atingiram a meta de LDL-C e as terapias utilizadas em um centro terciário; B) o impacto da utilização do método de Martin-Hopkins em vez do método de Friedewald na proporção de pacientes controlados. Métodos: Estudo transversal monocêntrico, incluindo pacientes consecutivos pós-infarto do miocárdio, acompanhados por 20 cardiologistas, em um hospital terciário. Os dados foram coletados retrospectivamente de consultas clínicas realizadas após abril de 2022. Para cada paciente, os níveis de LDL-C e o atingimento das metas foram estimados a partir de um perfil lipídico ambulatorial, utilizando as fórmulas de Friedewald e Martin-Hopkins. Um valor-p bicaudal < 0,05 foi considerado estatisticamente significativo para todos os testes. Resultados: Foram incluídos 400 pacientes (com 67 ± 13 anos, 77% do sexo masculino). Utilizando a fórmula de Friedewald, a mediana de LDL-C sob terapia foi de 64 (50-81) mg/dL, e 31% tinham LDL-C dentro da meta. Estatinas de alta intensidade foram usadas em 64% dos pacientes, 37% estavam em uso de ezetimiba e 0,5% estavam em uso de inibidores de PCSK9. A terapia combinada de estatina de alta intensidade + ezetimiba foi utilizada em 102 pacientes (26%). A aplicação do método de Martin-Hopkins reclassificaria um total de 31 pacientes (7,8%). Entre aqueles considerados controlados pela fórmula de Friedewald, 27 (21,6%) teriam LDL-C estimado por Martin-Hopkins acima da meta. Conclusões: Menos de um terço dos pacientes pós-infarto do miocárdio apresentaram LDL-C dentro da meta. A aplicação da fórmula de Martin-Hopkins reclassificaria um quinto dos pacientes presumivelmente controlados no grupo de pacientes não controlados. Fundamento LDLC C (LDL-C 5 mgdL mg dL estabelecida LDLC, C, MartinHopkins Martin Hopkins precisão Objetivos avaliar terciário B Métodos monocêntrico pósinfarto pós infarto 2 cardiologistas 2022 paciente ambulatorial MartinHopkins. Hopkins. valorp valor p 005 0 05 0,0 testes Resultados 40 6 1 anos 77 masculino. masculino . masculino) 5081 50 81 (50-81 37 0,5 PCSK9 PCSK 10 26%. 26 26% (26%) 3 7,8%. 78 7,8% 7 8 (7,8%) 21,6% 216 21 (21,6% Conclusões 202 00 0, 4 508 (50-8 (26% 7,8 (7,8% 21,6 (21,6 (50- (26 7, (7,8 21, (21, (50 (2 (7, (21 (5 ( (7

Abstract Background: Real-world, primary data on the treatment of psoriasis are scarce, especially concerning the role of soluble biomarkers as outcome predictors. Objective: The authors evaluated the utility of Th1/Th17 serum cytokines along with clinical characteristics as predictors of drug survival in the treatment of psoriasis. Methods: The authors consecutively included participants with moderate to severe psoriasis who were followed up for 6 years. Baseline interferon-α, tumor necrosis factor-α, and inter-leukin (IL)-2, IL-4, IL-6, IL-10, and IL-17A were measured using a cytometric bead array; clinical data were assessed. The authors calculated hazard ratios (HRs) for drug survival using a Cox proportional hazards model. Results: The authors included 262 patients, most of whom used systemic immunosuppressants or biologics. In the multivariate model, poor quality of life measured by the Dermatology Life Quality Index (HR = 1.04; 95% CI 1.01–1.07; p = 0.012) and elevated baseline IL-6 (HR = 1.99; 95% CI 1.29–3.08; p = 0.002) were associated with treatment interruption. Study limitations: The main limitation of any cohort study is the presence of confounders that could not be detected in clinical evaluation. Conclusions: Poor quality of life and elevated baseline serum IL-6 level predicted treatment interruption in patients with moderate to severe psoriasis. Although IL-6 is not the most important mediator of the inflammatory pathway in the skin environment, it is an interesting biomarker candidate for predicting psoriasis treatment response. Background Realworld, Realworld Real world, world Real-world scarce Objective Th1Th17 ThTh Th1 Th17 Th Th1/Th1 Methods years interferonα, interferonα interferon α, α interferon-α factorα, factorα factor factor-α interleukin inter leukin IL2, IL2 IL 2, 2 (IL)-2 IL4, IL4 4, 4 IL-4 IL6, IL6 6, IL10, IL10 10, 10 IL-10 IL17A ILA 17A A array assessed HRs (HRs model Results 26 biologics HR 1.04 104 1 04 95 1.01–1.07 101107 01 07 0.012 0012 0 012 IL- 1.99 199 99 1.29–3.08 129308 29 3 08 0.002 0002 002 limitations evaluation Conclusions environment response Th1Th1 Th1/Th (IL)- IL1 IL-1 1.0 9 1.01–1.0 10110 0.01 001 1.9 19 1.29–3.0 12930 0.00 000 00 Th1Th (IL) 1. 1.01–1. 1011 0.0 1.29–3. 1293 (IL 1.01–1 101 0. 1.29–3 129 1.01– 1.29– 12 1.01 1.29 1.2

Abstract Deep purple is an open-pollinated variety, well adapted to tropical conditions, developed to induce maternal haploids in maize. The founders were W23, Stock6, and an experimental tropical maize population. The inducer population inherits the dominant the R1-nj marker, and the expected real haploid induction rate is 3%. openpollinated open pollinated variety conditions W23 W Stock6 Stock R1nj Rnj R1 nj R marker 3 3% W2

Evaluamos en conjunto con profesionales de los servicios, los desafíos para la implementación del modelo biosicosocial en los centros especializados en rehabilitación. Por medio de la articulación de la Evaluación de Cuarta Generación y Rueda de Conversación (Método Paideia), abordamos los modelos implementados de asistencia a la persona con discapacidad, partiendo de las siguientes cuestiones: trabajar en rehabilitación; historial médico y evaluación; atención compartida y calificación del alta. Del contenido de las ruedas surgieron tres categorías empíricas: el proceso de trabajo en rehabilitación; el proceso en red; el modelo de cuidado. La identificación de obstáculos y facilitadores podrá favorecer la plena implementación del modelo biosicosocial en la atención a las personas con discapacidad, generando subsidios para el avance hacia la integralidad del cuidado a las personas con discapacidad representado en este modelo. servicios rehabilitación Método Paideia, Paideia , Paideia) cuestiones evaluación alta empíricas red

We assessed challenges in implementing the biopsychosocial model in specialized rehabilitation centers together with the professionals working in these services. Using fourth generation assessment and conversation circles (the Paideia method), we explored models of care for people with disabilities focusing on the following issues: working in rehabilitation; health records and evaluation; shared appointments; and improving discharge. Three empirical categories emerged from the conversation circles: rehabilitation work processes; the network-based approach; and the care model. The identification of constraints and enabling factors can help promote the effective implementation of the biopsychosocial model, generating important insights to help drive progress towards the delivery of comprehensive care to people with disabilities represented in this model. services method, method , method) issues evaluation appointments discharge processes networkbased network based approach

Avaliamos, em conjunto com profissionais dos serviços, os desafios à implementação do modelo biopsicossocial nos Centros Especializados em Reabilitação. Por meio da articulação da Avaliação de Quarta Geração e Roda de Conversa (Método Paideia), abordamos os modelos implementados de assistência à pessoa com deficiência, partindo das seguintes questões: trabalhar em reabilitação; prontuário e avaliação; atendimento compartilhado e qualificação da alta. Do conteúdo das rodas emergiram três categorias empíricas: o processo de trabalho em reabilitação; o trabalho em rede; o modelo de cuidado. A identificação de entraves e facilitadores poderá favorecer a plena implementação do modelo biopsicossocial na atenção às pessoas com deficiência, gerando subsídios para o avanço rumo à integralidade do cuidado às pessoas com deficiência representado neste modelo. Avaliamos serviços Reabilitação Método Paideia, Paideia , Paideia) questões reabilitação avaliação alta empíricas rede

Abstract At low concentrations used for in-office bleaching gels, such as 6% HP, gingival barrier continues to be performed. If we take into account that, in the at-home bleaching technique, no barrier is indicated, it seems that the use of a gingival barrier fails to make much sense when bleaching gel in low concentration is used for in-office bleaching. Objective This double-blind, split-mouth, randomized clinical trial evaluated the gingival irritation (GI) of in-office bleaching using 6% hydrogen peroxide (HP) with and without a gingival barrier in adolescents, as well as color change and the impact of oral condition on quality of life. Methodology Overall, 60 participants were randomized into which side would or would not receive the gingival barrier. In-office bleaching was performed for 50 minutes with 6% HP in three sessions. The absolute risk and intensity of GI were assessed with a visual analogue scale. Color change was assessed using a digital spectrophotometer and color guides. The impact of oral condition on quality of life was assessed using the Brazilian version of the Oral Health Impact Profile (α=0.05). Results The proportion of patients who presented GI for the “with barrier” group was 31.6% and for the “without barrier” group, 30% (p=1.0). There is an equivalence for the evaluated groups regarding GI intensity (p<0.01). Color change was detected with no statistical differences (p>0.29). There was a significant impact of oral condition on quality of life after bleaching (p<0.001). Conclusions The use or not of the gingival barrier for in-office bleaching with 6% HP was equivalent for GI, as well as for bleaching efficacy, with improvement in the impact of oral condition on quality of life. inoffice office gels 6 athome at home technique indicated doubleblind, doubleblind double blind, blind double-blind splitmouth, splitmouth split mouth, mouth split-mouth (GI (HP adolescents Overall Inoffice In 5 sessions scale guides α=0.05. α005 α α=0.05 . 0 05 (α=0.05) 316 31 31.6 30 p=1.0. p10 p p=1.0 1 (p=1.0) p<0.01. p001 p<0.01 01 (p<0.01) p>0.29. p029 p>0.29 29 (p>0.29) p<0.001. p0001 p<0.001 001 (p<0.001) efficacy α00 α=0.0 (α=0.05 3 31. p1 p=1. (p=1.0 p00 p<0.0 (p<0.01 p02 p>0.2 2 (p>0.29 p000 p<0.00 00 (p<0.001 α0 α=0. (α=0.0 p=1 (p=1. p0 p<0. (p<0.0 p>0. (p>0.2 (p<0.00 α=0 (α=0. p= (p=1 p<0 (p<0. p>0 (p>0. α= (α=0 (p= p< (p<0 p> (p>0 (α= (p (p< (p> (α

ABSTRACT Our objective was to evaluate the genetic potential of two maize populations derived from commercial hybrids for beginning a reciprocal recurrent selection (RRS) program. For this, 280 interpopulation half-sib (HS) progenies were produced from two populations (140 HS from each population), which were derived from the selfing of the commercial hybrids AS1598 (Pop1) and RB9210 (Pop2). We then evaluated the 280 HS progenies, the two base populations, and the two hybrids for grain yield (GY) and ear and plant architecture traits in southwestern Goias during the 2020 winter season. Variance components and genetic values of each progeny were estimated using the REML/BLUP (Restricted Maximum Likelihood/Best Linear Unbiased Prediction) methodology. Genetic gains by selection of the top 20% of progenies were predicted using two strategies: i) selection for GY and ii) selection for GY and days to silking (DTS) simultaneously. We observed genetic variability in both populations for all tested traits; therefore, it is possible to obtain genetic gains from the interpopulation improvement of both Pop1 and Pop2. The genetic gain estimates with the selection based on GY were 9.03 and 3.45% for Pop1 and Pop2, respectively. Simultaneous selection for GY and DTS resulted in positive (>4%) genetic gains for GY in both populations and decreased the cycle in Pop1, but we did not observe any alteration of the cycle in Pop2. We concluded that both populations derived from commercial hybrids have genetic potential for interpopulation improvement using RRS. RRS (RRS program this 28 halfsib half sib (HS 140 (14 population, population , population) AS AS159 Pop (Pop1 RB RB921 Pop2 . (Pop2) (GY 202 season REMLBLUP REML BLUP Restricted LikelihoodBest Likelihood Best Prediction methodology 20 strategies i ii (DTS simultaneously therefore 903 9 03 9.0 345 3 45 3.45 respectively >4% 4 (>4% 2 14 (1 AS15 (Pop RB92 (Pop2 90 0 9. 34 3.4 >4 (>4 1 ( AS1 RB9 3. > (>

RESUMO O objetivo deste trabalho foi avaliar o potencial genético de duas populações de milho derivadas de híbridos comerciais para iniciar um programa de seleção recorrente recíproca (SRR). Para isso, 280 progênies de meios-irmãos (PMI) interpopulacionais foram obtidas de duas populações (140 PMI de cada população) derivadas da autofecundação dos híbridos comerciais AS1598 (Pop1) e RB9210 (Pop2). As 280 PMI, as duas populações e os dois híbridos foram avaliados para produtividade de grãos (PG) e seis caracteres de arquitetura de planta e de espiga em dois locais no Sudoeste de Goiás, na segunda safra de 2020. Os componentes de variância e os valores genéticos de cada progênie foram estimados pela metodologia REML/BLUP (Restricted Maximum Likelihood/Best Linear Unbiased Prediction). Os ganhos genéticos com a seleção de 20% das progênies superiores foram preditos usando duas estratégias: i) seleção truncada para PG e ii) seleção simultânea para PG e florescimento feminino (FF). Observou -se variabilidade genética nas duas populações para todos os caracteres avaliados. Consequentemente, é possível obter ganhos genéticos a partir do melhoramento interpopulacional das Pop1 e 2. As estimativas de ganhos com a seleção truncada para PG foram de 9,03% e 3,45% para as Pop1 e Pop2, respectivamente. A seleção simultânea para PG e FF resultou em ganhos positivos (>4%) para PG em ambas populações e redução no ciclo na Pop1, mas não houve alteração para ciclo na Pop2. Conclui-se que as duas populações derivadas de híbridos comerciais de milho têm potencial genético para o melhoramento interpopulacional com emprego de SRR. SRR . (SRR) isso 28 meiosirmãos meios irmãos (PMI 140 (14 população AS AS159 Pop (Pop1 RB RB921 Pop2 (Pop2) (PG Goiás 2020 REMLBLUP REML BLUP Restricted LikelihoodBest Likelihood Best Prediction. Prediction Prediction) 20 estratégias i ii FF. (FF) se Consequentemente 2 903 9 03 9,03 345 3 45 3,45 respectivamente >4% 4 (>4% Concluise Conclui (SRR 14 (1 AS15 (Pop RB92 (Pop2 202 (FF 90 0 9,0 34 3,4 >4 (>4 1 ( AS1 RB9 9, 3, > (>

ABSTRACT Endophthalmitis is a severe form of purulent inflammation caused by the infection of the intraocular tissues or fluids. This infection infrequently occurs through endogenous routes, which are often correlated with major risk factors. Escherichia coli, a gram-negative rod, can cause endophthalmitis through hematogenous spread. We here report a 59-year-old man who presented to our service with acute visual impairment in his left eye, preceded by floaters. He was taking sirolimus and azathioprine for a transplanted kidney, had undergone catheterization for bladder atresia, and had a history of recurrent E. coli urinary tract infections. On evaluation, the left eye exhibited visual acuity of hand motion, anterior chamber reaction (3+/4+), and intense vitritis (4+/4+) with white flake clusters, which prevented appropriate retinal evaluation. Pars plana vitrectomy was performed, and the culture yielded E. coli. The present case highlights the importance of identifying the signs and symptoms of infection early so that diagnosis and treatment of endophthalmitis can be promptly initiated. fluids routes factors gramnegative gram negative rod spread 59yearold yearold 59 year old floaters kidney atresia E infections evaluation motion 3+/4+, 34 3+/4+ , 3 4 (3+/4+) 4+/4+ 44 (4+/4+ clusters performed initiated 5 3+/4 (3+/4+ 4+/4 (4+/4 3+/ (3+/4 4+/ (4+/ 3+ (3+/ 4+ (4+ (3+ (4 (3 (

ABSTRACT In Brazil, the COVID-19 burden was substantial, and risk factors associated with higher in-hospital mortality rates have been extensively studied. However, information on short-term all-cause mortality and the factors associated with death in patients who survived the hospitalization period of acute SARS-CoV-2 infection is limited. We analyzed the six-month post-hospitalization mortality rate and possible risk factors of COVID-19 patients in a single center in Brazil. This is a retrospective cohort study focused on a six-month follow-up. The exclusion criteria were death during hospitalization, transference to another hospital, and age under 18. We collected data from the charts of all hospitalized patients from March 2020 to December 2020 with a positive RT-PCR test for SARS-CoV-2, resulting in a sample size of 106 patients. The main outcome was death after hospitalization, whereas comorbidities and demographics were evaluated as risk factors. The crude post-hospitalization death rate was 16%. The first 30 days of follow-up had the highest mortality rate. In a Cox regression model for post-hospitalization mortality, previous chronic kidney disease (HR, 4.06, 95%CI 1.46 – 11.30) and longer hospital stay (HR 1.01, 95%CI 1.00 – 1.02) were the only factors statistically associated with death. In conclusion, a high six-month all-cause mortality was observed. Within the six-month follow-up, a higher risk of death was observed for patients who had prior CKD and longer hospital stay. These findings highlight the importance of more intensive medical surveillance during this period. Brazil COVID19 COVID 19 COVID-1 substantial inhospital studied However shortterm short term allcause cause SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- limited sixmonth six month posthospitalization post followup. followup follow up. up 18 202 RTPCR RT PCR SARSCoV2, 2, 10 16 16% 3 HR, HR 406 4 06 4.06 95CI CI 95 146 1 46 1.4 11.30 1130 11 101 01 1.01 100 00 1.0 1.02 102 02 conclusion followup, up, COVID1 COVID- SARS-CoV 20 40 0 4.0 9 14 1. 11.3 113 4. 11.

Abstract Studies investigating the effects of different modalities of exercises on the immune system are scarce. Therefore, the aim of this study was to compare the effects of eight weeks of resistance and aerobic training on the proliferation of T and B lymphocytes from mice. Eighteen male C56BL6/J mice were divided into groups (n=6), sedentary, aerobic, and resistance training. After 8 weeks, animals were euthanized, and their splenocytes were labeled and cultured with and without stimulation. Lymphocyte proliferation (CD4+, CD8+ and CD21/CD35+) was evaluated by flow cytometry. The mice subjected to resistance exercise exhibited greater proliferation for total, CD8+ and B lymphocytes (p<0.05), but not CD4+ cells (p>0.05), compared with their sedentary counterparts. We found significant correlations between maximum load and total, CD8+ and B lymphocytes proliferation rates (p<0.05). In conclusion, our results showed that resistance training promoted an improvement in the immune response associated with CD8+ and B lymphocytes. scarce Therefore C56BL6J CBLJ C56BL6 J C BL n=6, n6 n n=6 , 6 (n=6) euthanized stimulation CD4+, CD4 CD (CD4+ CD8 CD21/CD35+ CD21CD35 CDCD CD21 CD35 cytometry total p<0.05, p005 p p<0.05 0 05 (p<0.05) p>0.05, p>0.05 (p>0.05) counterparts p<0.05. . conclusion CBL C56BL n= (n=6 (CD4 CD21/CD35 CD21CD3 CD2 CD3 p00 p<0.0 (p<0.05 p>0.0 (p>0.05 (n= (CD CD21/CD3 CD21CD p0 p<0. (p<0.0 p>0. (p>0.0 (n CD21/CD p<0 (p<0. p>0 (p>0. p< (p<0 p> (p>0 (p< (p> (p

ABSTRACT Objective: To analyze the temporal trend of tuberculosis cure indicators in Brazil. Methods: An ecological time-series study using administrative data of reported cases of the disease nationwide between 2001 and 2022. We estimated cure indicators for each federative unit (FU) considering individuals with pulmonary tuberculosis, tuberculosis-HIV coinfection, and those in tuberculosis retreatment. We used regression models using joinpoint regression for trend analysis, reporting the annual percentage change and the average annual percentage change. Results: For the three groups analyzed, we observed heterogeneity in the annual percentage change in the Brazilian FUs, with a predominance of significantly decreasing trends in the cure indicator in most FUs, especially at the end of the time series. When considering national indicators, an average annual percentage change of −0.97% (95% CI: −1.23 to −0.74) was identified for the cure of people with pulmonary tuberculosis, of −1.11% (95% CI: −1.42 to −0.85) for the cure of people with tuberculosis-HIV coinfection, and of −1.44% (95% CI: −1.62 to −1.31) for the cure of people in tuberculosis retreatment. Conclusions: The decreasing trends of cure indicators in Brazil are concerning and underscore a warning to public authorities, as it points to the possible occurrence of other treatment outcomes, such as treatment discontinuity and death. This finding contradicts current public health care policies and requires urgent strategies aiming to promote follow-up of patients during tuberculosis treatment in Brazil. Objective Methods timeseries series 200 2022 FU (FU tuberculosisHIV HIV coinfection retreatment analysis Results analyzed FUs 097 0 97 −0.97 95% 95 (95 CI 123 1 23 −1.2 −0.74 074 74 111 11 −1.11 142 42 −1.4 −0.85 085 85 144 44 −1.44 162 62 −1.6 −1.31 131 31 Conclusions authorities outcomes death followup follow up 20 202 09 9 −0.9 (9 12 2 −1. −0.7 07 7 −1.1 14 4 −0.8 08 8 16 6 −1.3 13 3 −0. ( −1 −0 −

ABSTRACT Few Brazilian rural landowners are aware of the technology available to survey the subsoil of terrains earmarked for aquaculture operations. This study evaluated subsoils of Monte Alegre (area 1) and Montenegro 3 (area 2) aquaculture zones in the geographic region of Bragança (Amazon region, northern Brazil), and the adequacy of these areas for aquaculture. Ground Penetrating Radar, electrical conductivity measurements, and sedimentological analyses were applied to evaluate the subsoil of fish farms. Apparent conductivity values recorded by the Electromagnetic Induction (EMI) in area 1 indicated possible presence of clayey soils. Excavation analysis and sedimentological samples (sand) from this site confirmed the inadequacy of the terrain. The EMI tool in area 2 indicated possible presence of clayey soils. Geophysical and sedimentological results from the site confirmed its suitability. These geophysical tools are recommended for evaluating prospective aquaculture sites, given their capacity to provide reliable data on the subsoil characteristics , which is essential to guarantee the success and sustainability of aquaculture operations. operations Amazon Brazil, Brazil Brazil) Radar measurements farms (EMI soils sand (sand terrain suitability sites

Abstract The Brazil-Malvinas Confluence (BMC) is a significant biological frontier where distinct currents meet, fostering optimal conditions for phytoplankton development. In this study we tested the hypothesis that eddys promote an increase in phytoplankton biomass at the Brazil-Malvinas Confluence (BMC), altering species diversity. Phytoplankton were collected with Niskin bottles and nutrient concentrations assessed at two depths (Surface and Deep Chlorophyll Maximum Layer – DCML) in areas outside and under the influence of Cold-Core (CCE) and Warm-Core (WCE) Eddies. Environmental variables were determined in situ using a CTD profiler. Four regions were separated based on environmental variables and phytoplankton species, namely, the Brazil Current (BC), Malvinas Current (MC), CCE, and WCE. Species diversity was higher in the eddies. The conditions of the WCE were different from those of the CCE, with low temperature and salinity and high cell density values in the latter. The phylum Bacillariophyta was predominant in terms of species richness in all regions and was responsible for the higher cell density in the MC, while dinoflagellates were dominant in the BC and eddies. Therefore, eddy activity alters the structure, diversity and biomass of the phytoplankton community in the BMC. BrazilMalvinas BMC (BMC meet development BMC, , Surface DCML ColdCore Cold Core CCE (CCE WarmCore Warm (WCE Eddies profiler namely BC, (BC) MC (MC) eddies latter Therefore structure (BC (MC