Abstract Abstract: In Brazil, there are two species of Artocarpus that were introduced: Artocarpus altilis (Parkinson) Fosberg, known as fruta-pão, and Artocarpus heterophyllus Lam., known as jaca. Both are used as food and medicine. The objective of this work was to conduct a comparative anatomical and histochemical study between A. altilis and A. heterophyllus. Techniques of optical, polarized and scanning electron coupled to energy dispersive spectroscopy. The anatomical characterization showed the characters of general occurrence in the family Moraceae and of those that allow the differentiation of A. altilis and A. heterophyllus. The histochemistry revealed the sites of synthesis and/or storage of the metabolites. The chemical microanalyses brought new information about the chemical composition of crystals. The study provides pharmacobotanical data for the quality control of the species.

Manilkara zapota (L.) P. Royen, popularly known as sapoti or sapota (sapodilla), is a tree bearing an important fruit, in addition to different parts of the plant being widely used in folk medicine in the management of inflammation, pain, fevers, coughs, diarrhea, dysentery, among other ailments. This study aimed to conduct a pharmacobotany standardization study of M. zapota. Semi-permanent slides, containing transversal sections of stem, petiole, leaf blade and fruit; and paradermic sections of leaf blade were prepared, and analyzed by light microscopy. Histochemical tests were also performed in cross-sections of the leaf blade. Microscopic analysis allowed the identification of important elements in the diagnosis of the species; while the use of histochemical techniques on the leaf blade showed evidence of the presence of phenolic compounds, tannins, triterpenes and steroids, lipophilic compounds, starch, lignin and calcium oxalate crystals. The results presented contributed to characterization of the species.

ABSTRACT Solidago chilensis Meyen, belonging to the family Asteraceae, is a plant native to South America and the only representative of the genus in Brazil. This species is popularly known as “arnica” and is used to treat bruises, muscle pain and inflammation. The objective of this work was to contribute to the pharmacognostic standardization of S. chilensis. Cross-sections were obtained, by freehand, for microscopic analysis of root, stem and leaf; for these parts of the plant maceration was also performed according to the method of Jeffrey. For the leaf were still made paradermal sections, scanning electron microscopy analysis, phytochemical and histochemical tests. Thus, it was determined anatomical features useful for diagnosis of the species which, together with identification of the chemical compounds and its histolocalization, provides support to their quality control.

Abstract Inserted in one of the largest families among the Angiosperms, Genipa americana L., Rubiaceae, can be found in all Brazilian territory, presenting great medicinal importance, where several uses have been attributed. In view of this, this work has the purpose of analyzing the ultrastructural, biomineral, phytochemical and histochemical characteristics of the leaves of this native species from Brazil. For this, light microscopy, polarization and scanning electron microscopy techniques were used with X-ray scattering energy, associated to chromatographic and histochemical tests. The anatomical ultrastructural characteristics of the leaves detailed information about the type and arrangement of the cuticle, trichomes, surface and arrangement of the tissues that determine the botanical identity of this species. The phytochemical tests allowed determining their chromatographic pattern and histochemistry to determine the exact storage site of these substances in the leaf. It was observed that the characterization of the crystalline macro-pattern present in the analyzed species, as well as its exact elemental composition, can be considered an important differential diagnosis factor. The results characterize the leaves of this species in different aspects, being a native species and pharmacologically promising, with different popular uses and proven pharmacological activities, and more in depth studies is needed.

ABSTRACT Ageratum conyzoides L., belonging to the family Asteraceae, is a tropical plant found in some regions of Africa, Asia and South America. This species is popularly known as billy goat weed, “mentrasto” and “catinga-de-bode” and has a large variety of secondary metabolites and biological activities mentioned in the literature. The objective of this work was to contribute the pharmacobotanical standardization of A. conyzoides. Cross-sections were obtained, by hand, for microscopic characterization of root, stem, petiole and leaf blade; to the leaf blade were still made paradermal and longitudinal sections, scanning electron microscopy analysis and maceration. The analysis showed that secretory structures ducts are evidenced only in the petiole and the leaf blade. The root has parenchymatous medullar region; stem, petiole and leaf blade exhibit striated cuticle. Non-glandular trichomes are present in stem, petiole and leaf blade, while capitate glandular trichomes are present only in the leaf blade and are restricted to the abaxial face. These anatomical features are useful for diagnosis of the species and provide support to their quality control.

ABSTRACT Dysphania ambrosioides (L.) Mosyakin & Clemants (syn: Chenopodium ambrosoides L.), Amaranthaceae, popularly known as “mastruz”, is an herb widely used in Brazil as anthelmintic. To contribute to the knowledge about medicinal plants, a microscopic analysis was accomplished to describe the main anatomical characters of root, stem, petiole and leaf blade of D. ambrosioides and histochemical tests were performed on the leaf blade. Cross-sections were obtained, by hand, for microscopic analysis of root, stem, petiole and leaf blade; to the leaf blade were still made paradermal sections, scanning electron microscopy analysis, maceration and histochemical tests. The main characters useful in the identification of the plant were: anomalous secondary thickening in the root and stem; presence of idioblasts containing crystal sand in the root, stem, petiole and leaf blade; in these there are also idioblasts with druses; presence of non-glandular and glandular trichomes in the stem, petiole and leaf blade; stomata on the stem, petiole and leaf blade, identified in these as anomocytic and anisocytic; dorsiventral mesophyll and collateral vascular bundles. Maceration revealed that the vessel elements are helical type. Through the histochemical tests, it was evidenced the presence of lipophilic substances, essential oils, oleoresins, phenolic compounds, starch, lignin and calcium oxalate crystals. This work provides support to the quality control of the species.

The roots from Operculina macrocarpa (L.) Urb., Convolvulaceae, are widely used in Brazilian traditional medicine as a laxative and purgative. The biological properties of this drug material have been attributed to its polysaccharides content. Thus, the aim of this study was to evaluate the polysaccharide content in drug material from O. macrocarpa by spectrophotometric quantitative analysis. The root was used as plant material and the botanical identification was performed by macro and microscopic analysis. The plant material was used to validate the spectrophotometric procedures at 490 nm for the quantification of the reaction product from drug polysaccharides and phenol-sulfuric acid solution. The analytical procedure was evaluated in order to comply with the necessary legal requirements by the determination of the following parameters: specificity, linearity, selectivity, precision, accuracy and robustness. This study provides with a simple and valid analytical procedure (linear, precise, accurate and reproducible), which can be satisfactorily used for quality control and standardization of herbal drug from O. macrocarpa.

The Simaroubaceae family includes 32 genera and more than 170 species of trees and brushes of pantropical distribution. The main distribution hot spots are located at tropical areas of America, extending to Africa, Madagascar and regions of Australia bathed by the Pacific. This family is characterized by the presence of quassinoids, secondary metabolites responsible of a wide spectrum of biological activities such as antitumor, antimalarial, antiviral, insecticide, feeding deterrent, amebicide, antiparasitic and herbicidal. Although the chemical and pharmacological potential of Simaroubaceae family as well as its participation in official compendia; such as British, German, French and Brazilian pharmacopoeias, and patent registration, many of its species have not been studied yet. In order to direct further investigation to approach detailed botanical, chemical and pharmacological aspects of the Simaroubaceae, the present work reviews the information regarding the main genera of the family up to 2013.

Acanthospermum hispidum DC., Asteraceae, is widely used in folk medicine in Brazil to treat respiratory diseases; this biological property has been attributed to its phytosterol content. This study evaluated the spectrophotometric assay method to quantify the total phytosterol content in raw materials and extracts from roots of A. hispidum. The procedure was based on the quantification at 625 nm after the Liebermann-Burchard reaction. The method was evaluated for linearity, repeatability, intermediate precision, accuracy and robustness. The date indicated that the procedure is a valid analytical tool for materials and herbal derivatives from A. hispidum.

Erythrina velutina Willd., Fabaceae, known as "mulungu", is a tree of tropical regions, as northeastern Brazil. Its bark is used in folk medicine as tranquilizer, sedative and insomnia. This study aimed to characterize the stem and leaf anatomy and to provide subsidies to quality control of the plant drug due to its wide use in folk medicine as well as its differentiation from other species with the same popular name. Samples were collected at Cuité, in Paraíba State, Brazil, fixed in FAA50, semipermanent slides were made, following usual procedures in plant anatomy. The stem shows a cylindrical contour, covered by a uniseriate epidermis covered by a thickened cuticle. It shows claviform glandular and branched trichomes with uniseriate stalk. Secretory cavities are into the phloem. The leaf epidermis has branched and glandular trichomes and anisocytic and paracytic stomata, on both sides, with predominance of branched trichomes and stomata on abaxial surface. Secretory cavities in stem and leaf, types of trichomes and stomata, its location and distribution constitute diagnostic characters for this specie. The structural characterization of the stem and leaf allows its distinction from other ones of this genus, ensuring safety for commercial pharmacological uses, allowing certification of the authenticity of raw material.

Este estudo descreve a anatomia e analisa a histoquímica dos órgãos vegetativos de Priva lappulacea (L.) Pers. Secções transversais e paradérmicas, à mão livre, de material fresco e fixado foram utilizadas para as análises anatômicas e histoquímicas, usando microscopia óptica. Os caracteres anatômicos são comuns àqueles descritos para a família, podendo ser utilizados como diagnóstico na sua identificação. Destacam-se, nas folhas, os tricomas, glandular e não-glandular, estômatos anomocíticos, em ambas as faces da epiderme, e mesofilo dorsiventral. As expansões no pecíolo são acrescentadas à diagnose da espécie. Estudo fitoquímico realizado com as partes aéreas mostrou a presença de triterpeno (ácido ursólico) e esteróide (β-sitosterol), iridóides (ipolamida e catalpol), açúcar redutor (glucose), flavonóide (luteolina) e fenilpropanóide (verbascosídeo). Nas raízes foi encontrada apenas glucose e dois iridóides estiveram presentes no caule e folhas. Alcalóides, saponinas, cumarinas, derivados cinâmicos, proantocianidinas condensadas e leucoantocianidinas não foram constatados nas partes analisadas. A descrição anatômica e os testes histoquímicos são inéditos para P. lappulacea.

This study describes the anatomy and analyzes the histochemistry of the vegetative organs of Priva lappulacea (L.) Pers. Freehanded transversal and paradermal sections of fresh and fixed material were used for the anatomical and histochemical analyses, using optical microscopy. The anatomical characters are common to those described for the family, being able to be used to diagnosis in its identification. The leaves are highlighted by the presence of glandular and non-glandular trichomes, anomocytic stomata on both faces of the epidermis and dorsiventral mesophyll. The expansions in the petiole are added to the diagnosis of this species. The phytochemical study carried out with aerial organs showed the presence of triterpene (ursolic acid) and steroid (β-sitosterol), iridoids (ipolamide and catalpol), reducing sugar (glucose), flavonoid (luteolin) and fenilpropanoid (verbascosides). In the roots only glucose was found and two iridoids are present in stem and leaves. Alkaloids, saponins, cumarines, cinamic acid derivates, condensed proantocianidines and leucoantocianidines were not checked in the analyzed parts. The anatomical description and the histochemical tests are unpublished for P. lappulacea.

A planta "Espinho-de-cigano" (Acanthospermum hispidum DC) é amplamente usada no nordeste do Brasil como medicamento popular para a asma. Embora muito pouco seja conhecido atualmente sobre a eficácia e segurança deste extrato vegetal, é possível encontrar numerosos medicamentos preparados com ele nos serviços públicos ou em lojas que vendem produtos naturais. Este estudo visa proceder a uma revisão de literatura relativa à A. hispidum, no período entre 1926-2006, nas áreas de etnobotânica, fitoquímica e farmacologia. O objetivo foi contribuir para um melhor conhecimento desta espécie e seus usos, assim como auxiliar na melhora de seu desempenho como um medicamento natural. A espécie é facilmente identificável e cresce abundantemente durante a estação chuvosa no nordeste do Brasil; é possível cultivá-la sem perda de seu perfil fitoquímico e os estudos toxicológicos têm mostrado sua segurança como um medicamento (embora mais estudos sejam requeridos nestes aspectos). Assim sendo, a compilação do conhecimento acumulado relativo a esta espécie auxiliará na avaliação de seu valor farmacológico, garantindo o controle de qualidade do produto final, e no preparo das recomendações para usos e dosagens que ofereçam segurança e eficiência ao usuário.

The "Espinho-de-cigano" plant (Acanthospermum hispidum DC) is used throughout northeastern (NE) Brazil as a folk medicine for asthma. Although little is actually known about the effectiveness and safety of this plant extract product, it is possible to find numerous medicines prepared from it in public health services or in stores selling natural products. This study aimed to undertake a literature review relative to Acanthospermum hispidum covering the period between 1926-2006 within the areas of ethnobotany, phytochemistry and pharmacology. The objective was to contribute to a better knowledge of this species and its uses, as well as to aid in improving its performance as a natural medicine. The species is easily identifiable and grows abundantly during the rainy seasons in NE Brazil; is amenable to cultivation without lose of its phytochemical profile, and toxicological studies have showed its safety as a medicine (although more studies will be required in that direction). As such, the compilation of the accumulated knowledge concerning this species will aid in evaluating its pharmacological value, guaranteeing quality control of the final product, and in preparing recommendations for usages and dosages that offer both safety and efficiency to the user.

Própolis é uma mistura complexa, formada por material resinoso e balsâmico. Sua composição química é complexa e variada, estando relacionada com a flora de cada região visitada pelas abelhas e com o período de coleta da resina. Inclui flavonóides, ácidos aromáticos, terpenóides e fenilpropanóides, ácidos graxos e vários outros compostos. A própolis tem sido objeto de intensos estudos farmacológicos e químicos nos últimos 30 anos. Em várias partes do mundo é indicada para melhorar a saúde e prevenir doenças. Atualmente, é disponível em várias formas farmacêuticas como cápsulas, extratos, enxaguatório bucal, na forma de pó, entre outras. Ainda são necessários estudos correlacionando a composição química com a atividade biológica, definindo cada tipo de própolis com a sua aplicação terapêutica. É uma tarefa imprescindível para um mercado cada vez maior e exigente em todo o mundo.

Propolis (bee glue) is a complex mixture, formed by resinous and balsamic material. Its chemical composition is variable and complex, being related with the flora of each region visited by the bees and with the period of resins collection. Flavonoids, aromatic acids, terpenoids and phenylpropanoids, fatty acids, and other compounds are found in propolis. In the last 30 years, propolis has become subject of intense pharmacological and chemical studies. In different parts of the world it is indicated to improve health and prevent illnesses. Currently, it is available in some pharmaceutical forms as capsules, extracts, mouthrinses, powder form, among others. Indeed, studies correlating the chemical composition with the biological activity are necessary, defining each type of propolis with its therapeutic application. It is an essential task for a market ever bigger and demanding in the whole word.