Resumo O Brasil é o maior produtor de figos da América do Sul, porém o cultivo comercial brasileiro da figueira baseia-se na plantação de uma única cultivar, o Roxo-de-Valinhos, resultando em sérios problemas relativos a pragas e doenças. Uma vez que há variações epigenéticas na interação planta-patógeno, principalmente por meio da regulação gênica, o presente trabalho objetiva realizar a caracterização in vivo de acessos de figo, por meio da análise de incidência natural de nematoides formadores de galha e de incidência natural de ferrugem, correlacionada ao seu perfil epigenômico, a fim de subsidiar trabalhos de conservação, melhoramento genético e produção da cultura. A análise dos componentes principais dos dados da caracterização dos acessos foi realizada por meio da matriz de correlação residual obtida pela análise de variância conjunta utilizando o programa GENES. Pôde-se constatar que todas as plantas foram atacadas por este patógeno, evidenciando que são suscetíveis ao mesmo. Já a análise conjunta dos dados demonstrou que a metilação e a incidência de ferrugem em folhas, quando observadas na mesma fase fenológica da planta, se correlacionam, apresentando evidências iniciais de mesmas cargas fatoriais de pressão nos genótipos, com a premissa de comportamento semelhante nos mesmos, indicando que, além do fator genético, fatores abióticos também são responsáveis pelas alterações no fenótipo das plantas, evidenciando a plasticidade fenotípica das mesmas.

Abstract Commercial fig tree cultivation in Brazil involves a single cultivar, ‘Roxo-de-Valinhos’. The use of a single cultivar results in serious diseases and related problems. The aim of this study was to characterize fig accessions by analyzing the natural root-knot nematode and leaf rust incidence in relation to the epigenomic profile of the plant, since epigenetic variations affect plant–pathogen interactions. All plants were attacked by nematodes, indicating susceptibility; Meloidogyne incognita was the root-knot nematode species involved. Joint analysis of data showed that methylation and leaf rust incidence were correlated when observed in the same phenological phase, presenting initial evidence of the same factorial pressure loads in genotypes, suggesting similar behavior within these genotypes.

Abstract The influence of the sand proportion in mortars Type II was studied, according to the standard N-CMT-2-01-004/02, using three fine aggregates from the region of Morelia, in Michoacan, and determining their influence in the physico-mechanical properties, while considering the use of different cementitious materials (CM): Portland cement plus lime (B1), and Portland cement plus construction cement (B2). Mortar mixtures were designed for B1 and B2 with volume ratios of aggregates/CM, from 2.25 to 4, determining the fluency, the uniaxial compressive strength (UCS) and the wet electrical resistivity (WER). The relation between the fluency and the ratio water/CM were studied, concluding that the aggregate type and the proportions employed modify the fluency and the water demand in the fresh state of the mortar, and consequently impact the UCS and WER.

Resumo As proporções de areia nas argamassas de tipo II foram analisadas de acordo com a norma N-CMT-2-01-004/02. Foram utilizados três tipos de agregados da região de Morelia, Michoacán para determinar a sua influência nas suas propriedades físico-mecânicas. Considerando a utilização de diferentes materiais cimentícios (CM): cimento Portland mais cimento de alvenaria (B2) e cimento Portland mais cal (B1). Foram preparadas misturas para B1 e B2 com relações areia/MC de 2,25 para 4. Foram determinadas a fluidez, resistência uniaxial à compressão (UCS) e resistividade elétrica úmida (WER). Estudando a relação entre a fluidez e a quantidade de água/MC, concluindo que o tipo de agregado e as proporções modificam a fluidez e a demanda de água no estado fresco com impacto no UCS e no WER.

Resumen Se analizó la influencia de la proporción de arena en morteros Tipo II según norma N-CMT-2-01-004/02 empleando tres tipos de áridos de la región de Morelia, Michoacán determinando su influencia en sus propiedades físico-mecánicas, contemplando el uso de diferentes materiales cementantes (MC): el cemento Portland más cal (B1), cemento Portland más cemento de albañilería (B2). Se elaboraron mezclas para B1 y B2 con relaciones en volumen arena/MC, desde 2.25 hasta 4, determinando la fluidez, resistencia a compresión uniaxial (UCS) y resistividad eléctrica húmeda (WER). Se estudió la relación entre la fluidez y la cantidad de agua/MC, concluyendo que el tipo de árido y proporciones modifican la fluidez y demanda de agua en estado fresco impactando en UCS y WER.

ABSTRACT The almond (Prunus dulcis) is an economically important crop in Portugal and is in the process of expansion. The assessment of the diversity of arthropods existing in the almond tree canopy becomes essential to know the trophic relationships that are established within it. The aim of this work was to identify the main arthropods present in the almond tree canopy in different soil management systems and water conditions. The trial was carried out in two almond groves, in Corujas, Macedo de Cavaleiros: rainfed and irrigated. They were subdivided into two treatments: 1) mobilized soil and 2) spontaneous vegetation, each with 3 blocks and 5 replications. Modalities 1 and 2 were installed in May 2022. Samples were collected fortnightly, between July and September 2022, performing 6 taps/tree/modality, using the tapping technique, to assess the auxiliary fauna and 20 leaves/ tree to monitor Monosteira unicostata (Hemiptera: Tingidae), Panonychus ulmi (Acari: Tetranychidae) and Tetranychus urticae (Acari: Tetranychidae). Posteriorly, the individuals were counted and their identification was made at order and family level, for the auxiliary fauna and at species level for the pests. The most frequent orders were Hymenoptera (30%) and Araneae (18%). There were no significant differences in the number of arthropods between treatments in the two almond groves. With regard to pests, T. urticae and M. unicostata predominated in both orchards, with greater abundance in the irrigated grove.

RESUMO A amendoeira (Prunus dulcis) é uma cultura economicamente importante em Portugal e atualmente encontra-se em fase de expansão. A avaliação da diversidade de artrópodes existente na copa da amendoeira torna-se essencial para conhecer as relações tróficas que se estabelecem no seu interior. O objetivo deste trabalho foi identificar os principais artrópodes presentes na copa das amendoeiras em diferentes sistemas de gestão do solo e condições hídricas. O ensaio foi conduzido em dois amendoais, em Corujas, Macedo de Cavaleiros: sequeiro e regadio. Eles foram subdivididos em dois tratamentos: 1) solo mobilizado e 2) vegetação espontânea, cada um com 3 blocos e 5 repetições. As modalidades 1 e 2 foram instaladas em maio de 2022. As amostras foram recolhidas quinzenalmente, entre julho e setembro de 2022, realizando-se 6 batidas/árvore/modalidade, na técnica das pancadas, para avaliar a fauna auxiliar e colhidas 20 folhas/árvore para monitorizar Monosteira unicostata (Hemiptera: Tingidae), Panonychus ulmi (Acari: Tetranychidae) e Tetranychus urticae (Acari: Tetranychidae). Posteriormente, contaram-se os indivíduos e a sua identificação foi feita ao nível de ordem e família para a fauna auxiliar e de espécie para as pragas. As ordens mais frequentes foram Hymenoptera (30%) e Araneae (18%). Não se verificaram diferenças significativas no número de artrópodes entre os tratamentos, nos dois amendoais. Quanto às pragas, predominaram T. urticae e M. unicostata nos dois amendoais, verificando-se maior abundância no amendoal de regadio.

Abstract This work, subdivided into the first part and the second part, produced a technographic overview of the lithic material of middle Holocene sites, located in the central portion of South America, in the macro-region dominated by savannas in the lowlands of South America. This analysis was possible through reflection on available bibliographic information. The description of the modes of making lithic tools was based on the recognition of the three technical principles based on the techno-functional perspective: debitage, shaping and affordance; and on the considerations about relationship between the active and prehensive parts of the pieces (also called “techno-functional categories”). As a result, a synthesis of the technological peculiarities and variabilities was obtained in areas little known or documented with regard to the lithic materiality. In the first part of the article, this overview highlighted that there was, in the groupings of the sites of the Cratón Amazônico, a localized contribution to the relative technical cultures on the part of the principles of debitage and shaping, without being completely homogeneous. In reference to the management of raw materials, a local supply of lithic resources can be seen near these sites.

Resumen Este trabajo, subdividido em primera y segunda parte, presenta un panorama tecnográfico del material lítico proveniente de sitios del Holoceno medio, localizados en la región central de América del Sur, específicamente de la macro-región dominada por sabanas en las tierras bajas. Este análisis fue posible a través de una revisión y reflexión sobre la información bibliográfica disponible. En base a un enfoque tecno-funcional, la descripción de los modos de manufactura de útiles líticos se basó en el reconocimiento de tres principios técnicos desde el punto de vista tecno-funcional: débitage, façonnage y affordance, así como de la consideración de la relación entre las porciones activas y prehensivas de las piezas interpretadas como útiles (denominadas también “categorías tecno- funcionales”). Como resultado se obtuvo una síntesis de las particularidades y variabilidades tecnológicas en áreas poco conocidas o documentadas en lo que se refiere a la materialidad lítica. En la primera parte del artículo, esta síntesis puso en evidencia que en los agrupamientos de los sitios del Cratón Amazónico existió un conjunto de culturas tecnicas relativamente homogéneas concentradas alrededor de los principios de débitage y façonnage. En cuanto a la gestión de la materia prima, se observa en estos sitios un aprovisionamiento local de recursos líticos.

Abstract This article constitutes the second part of the lithic technographic analysis of Middle Holocene sites in a vast macro-region, the central portion of South America: it includes the groups of sites B, C and D. A common element in all the analyzed sets is the presence of the techno-functional category B tools. In reference to the production mode, it is observed that the principle of debitage predominates in the area of analysis. However, its application was diverse; there are assemblages in which there was a predetermination about the making of flakes, others in which the cores were reintroduced as blanks or presented edges and there are other places in which façonnage confection flakes were reintroduced as blanks for tools. Other production principles have also been recorded: the principles of affordance (both in the exclusive productional expression and in its productional and functional expression) and shaping are observed in sites in groups A, C and D. Recognizing the different production modes is the starting point for thinking about how groups with different technical traditions have occupied different spaces in the region.

Resumen Este artículo conforma la segunda parte del análisis de las tecnografías líticas de los sitios del Holoceno medio en una extensa macrorregión, el sector central de América del Sur: abarca los agrupamientos B, C y D de los sitios. Un elemento común en todos los conjuntos analizados en la presencia de herramientas de la categoría tecno-funcional B. En referencia a los modos de producción se observa que el principio de debitagem predomina en el área de análisis. No obstante su aplicación es diversa; hay conjuntos donde existió una predeterminación sobre la confección de lascas, otros donde los núcleos son reintroducidos como soportes o presentando filos y otros sitios en donde se reintrodujeron lascas de confección de façonage como herramientas de soporte. El resto de los principios de producción también fueron registrados: el de afordancia (tanto en la producción instrumental como en su expresión produccional) y el de façonage se observan en sitios de los agrupamientos A, C y D. El reconocimiento de los diferentes modos de producción, es el punto de inicio para pensar como grupos con tradiciones técnicas diferentes, ocuparon espacios diversos en la región.

The worldwide emergence of viral diseases such as Zika, Dengue, Chikungunya, West Nile and Yellow Fever urge the search for solutions to eliminate their common vector, the Aedes aegypti mosquito. This paper describes the quantitative structure-activity relationship (QSAR) and docking studies of a series of nine 3-(3-aryl-1,2,4-oxadiazol-5-yl)propionic acids (AOPA), 1-9, previously published by our group. Additionally, three new 1,2,4-oxadiazoles, 10-12, have also been synthesized, characterized and studied. The QSAR and docking studies of all compounds, 1-12, clearly indicate that larger hydrophobic substituents such as biphenyl groups attached on position 3 in 1,2,4-oxadiazoles improve the larvicidal activity. It is worthwhile to mention that nanocapsulation of compounds 10-12 were necessary to help their dissolution in water and these three new 1,2,4-oxadiazoles also exhibited approximately equal or higher larvicidal activities compared to the former prototypes at stage L4.

Resumen Se realizaron recolectas de mosquitos (Diptera: Culicidae) en el intradomicilio y peridomicilio a lo largo de la frontera Chiapas-Guatemala durante octubre-noviembre del 2018. Se recolectaron 4,439 especímenes pertenecientes a 2 subfamilias, 18 géneros y 41 especies, una de ellas Mansonia dyari (Belkin, Heinemann y Page, 1970) es un nuevo registro estatal para Chiapas. De las 41 especies recolectadas, 21 tienen importancia en salud pública, siendo algunas de ellas los principales vectores de malaria, dengue, fiebre amarilla, chikungunya, zika, mayaro, encefalitis equina venezolana, encefalitis equina del oeste y fiebre del valle del Rift. Culex quinquefasciatus y Aedes aegypti mostraron ser las más abundantes. En algunos casos, los municipios con mayor número de especies e individuos no fueron los más diversos y equitativos, con una alta disimilitud en la composición de sus especies. El intradomicilio obtuvo las mayores abundancias de mosquitos, sin embargo, su diversidad y equitatividad fue baja a diferencia del peridomicilio, presentándose una disimilitud ligeramente alta entre sus especies. Los resultados obtenidos tienen importancia para el establecimiento de programas de vigilancia y control en los municipios fronterizos en el estado de Chiapas, ya que brindan información base de la presencia y distribución de la fauna de mosquitos.

Abstract Mosquito collection was conducted intradomiciliary and peridomiciliary along the Chiapas-Guatemala international border during October-November 2018. We collected 4,439 specimens belonging to 2 subfamilies, 18 genera and 41 species, one of which Mansonia dyari (Belkin, and Page, 1970) is a new record for Chiapas, and 21 species with medical importance were found. Culex quinquefasciatus and Aedes aegypti were the most common species. Some municipalities with the highest number of species and individuals were not the most diverse and equitable, with a high dissimilarity in species composition. The intradomiciliary collections obtained the highest abundances of species, however the diversity and evenness were low in contrast with the peridomiciliary collections, where obtained slightly high dissimilarity among species. The results obtained have importance for the establishment of surveillance and mosquito control programs in the state of Chiapas, since they provide basic information on the occurrence and distribution of mosquito fauna.

Abstract A plethora of prolonged COVID-19 symptoms, or late manifestations has been reported after acute disease and labeled “post-COVID”. The aim of this study was to identify the prevalence of and risk factors for post-COVID up to 12 weeks after the onset of acute COVID-19. An electronic survey was conducted to evaluate post-COVID-19 symptoms, disease severity, demographics, and pre-existing diseases. The participants were recruited through 88,648 SMS messages, and post on social media. The associations between variables were explored through multivariate models. From 6,958 respondents with confirmed COVID-19, 753 (10.8%) required hospitalization, and 5,791 (83.2%) exhibited at least one post-COVID manifestation. Hair loss (49.4%), memory loss (40.7%), low attention (37.0%), fatigue (34.2%), anxiety (31.2%), and headache (29.6%) were the most reported post-COVID manifestations. Female sex, myalgia, anosmia, and severe disease were associated with most post-COVID manifestations. Pre-existing depression was associated with the development of neuropsychiatric manifestations. Post-COVID manifestations were identified in most patients following COVID-19 infection, placing a supplementary burden on the healthcare system. Hair loss, fatigue, and neuropsychiatric symptoms were the most prevalent post-COVID manifestations. Female sex, myalgia, anosmia, and more severe disease are risk factors for multiple post-COVID manifestations.

Ursolic acid (UA) is being investigated due to its anti-inflammatory potential, and structural modifications can enhance its biological activities. The aim of this study was to evaluate the immunomodulatory effect of the ursolic acid derivatives (UAD) in macrophages and in the carrageenan-induced paw edema model. RAW264.7 cells were cultured in the presence or absence of UA or UAD (1-18). Nitric oxide (NO), nuclear factor kappa B (NF-κB), tumor necrosis factor (TNF), and cellular viability were measured. 30 min before the carrageenan-induced paw edema, the UAD1 and UAD2 (200 mg kg-1) were administered intraperitoneally. The results showed that UAD2-4, UAD7, UAD9-11 had half maximal inhibitory concentration (IC50) greater than 90 µM and were able to reduce NO, NF-κB and TNF production. Moreover, UAD1 and UAD2 reduced paw edema and IL-6 production. In conclusion, the results obtained demonstrated a variation in the response between the derivatives due to the chemical modifications, showing potential to reduce the inflammatory mediators, deserving further investigations.

Abstract Purpose: The research analyzed the perception of entrepreneurs in the cities of São Paulo and São Luis regarding if the public policies implemented during the pandemic were sufficient to face the Covid-19 crisis and how they contributed to the survival of micro, small and medium-sized enterprises (MSMEs), in adverse situations. Originality/value: Faced with the advance of the Covid-19 contagion curve, governments worldwide have adopted measures to contain the disease. Such efforts negatively impacted organizations, especially MSMEs, which are more susceptible to the effects of the crisis. In these situations, the role of the State in promoting public policies to face the crisis is fundamental. It can represent the difference between survival and recovery versus the closure of business activities. Design/methodology/approach: A qualitative research with an exploratory, descriptive approach was developed through an empirical study carried out in two stages, combining the answers of a questionnaire in Google Forms and interviews with 48 entrepreneurs from MSMEs in the cities of São Paulo and São Luís. Content analysis was supported by Atlas.ti software. Findings: Five categories of public policies were created: 1. financial, 2. tributaries, 3. operational, 4. politicians, and 5. health. In adverse situations, government support is essential for the survival and recovery of MSMEs. It was concluded that although the support policies have been considered insufficient by surveyed entrepreneurs, those who managed to gain access reported that they were essential for maintaining the business.

Resumo Objetivo: A pesquisa analisou a percepção dos empreendedores das cidades de São Paulo e São Luís em relação às políticas públicas implementadas durante a pandemia para o enfrentamento da crise da Covid-19 e como elas contribuíram para a sobrevivência das micro, pequenas e médias empresas (MPME) em situações adversas. Originalidade/valor: Ante o avanço da curva de contágio da Covid-19, os governos de todo o mundo adotaram medidas de contenção da doença. Tais medidas trouxeram impactos negativos para as organizações, em especial para as MPME, que são mais suscetíveis aos efeitos da crise. Nessas situações, o papel do Estado na promoção de políticas públicas de enfrentamento da crise é fundamental e pode representar a diferença entre a sobrevivência e recuperação e o encerramento das atividades do negócio. Design/metodologia/abordagem: Uma pesquisa qualitativa com abordagem exploratória de caráter descritivo foi desenvolvida por meio de um estudo empírico realizado em duas etapas, combinando as respostas de um questionário no Google Forms e entrevistas com 48 empreendedores de MPME nas cidades de São Paulo e São Luís. A análise de conteúdo foi apoiada pelo software Atlas.ti. Resultados: Cinco categorias de políticas públicas foram criadas: 1. financeiras, 2. tributárias, 3. operacionais, 4. políticas e 5. de saúde. Em situações adversas, o apoio governamental é fundamental para a sobrevivência e recuperação das MPME. Concluiu-se que, embora as políticas de apoio tenham sido consideradas insuficientes pelos empreendedores pesquisados, aqueles que conseguiram ter acesso relataram que elas foram fundamentais para a manutenção do negócio.

Abstract The scale mealybug, Diaspis echinocacti (Bouché, 1833) (Hemiptera: Diaspididae), is one of the main pests of the cactus pear in Brazil. The objective was to study biological aspects of D. echinocacti at the constant temperatures of 25, 28, 30, 33 and 35 °C with relative humidity of 60 ± 10% and photoperiod of 12 hours in the laboratory on the cactus pear cultivar, “Orelha de Elefante Mexicana”, Opuntia stricta [Haw.] Haw. The development period (22 to 35 days) and survival in the egg (92 to 100%) and nymph (21.8 to 100%) stages and of the egg-adult cycle (20 to 100%), longevity (34.1 to 59.6 days) and fecundity (33 to 112 eggs) of D. echinocacti females with the different temperature and absence of males at the highest temperatures (> 30°C), indicated that the range between 25 °C and 30°C is the most favorable for this scale mealybug. This information may help to improve integrated management programs for D. echinocacti, in areas subject to seasonal temperature changes in the Brazilian regions where cactus pear is cultivated.

Resumo A cochonilha-escama, Diaspis echinocacti (Bouché, 1833) (Hemiptera: Diaspididae), é uma das principais pragas da palma forrageira no Brasil. O objetivo foi estudar aspectos biológicos de D. echinocacti nas temperaturas constantes de 25, 28, 30, 33 e 35 °C, umidade relativa de 60 ± 10% e fotoperíodo de 12 horas em laboratório na cultivar de palma forrageira, “Orelha de Elefante Mexicana”, Opuntia stricta [Haw.] Haw. O período de desenvolvimento (22 a 35 dias) e a sobrevivência nas fases de ovo (92 a 100%) e ninfa (21,8 a 100%) e o ciclo ovo-adulto (20 a 100%), longevidade (34,1 a 59,6 dias) e fecundidade (33 a 112 ovos) de fêmeas de D. echinocacti nas diferentes temperaturas e ausência de machos nas maiores (> 30°C) indicam ser a faixa entre 25°C e 30°C a mais favorável para esta cochonilha de escama. Essas informações podem auxiliar no aprimoramento de programas de manejo integrado de D. echinocacti em áreas sujeitas a variações sazonais de temperatura nas regiões brasileiras onde a palma forrageira é cultivada.

Abstract In this investigation thermochemical properties of 3-hydroxyphthalic anhydride were determined. Fusion enthalpy, fusion temperature and molar heat capacity in solid phase were obtained by differential scanning calorimetry. By the Knudsen effusion method, the molar enthalpy of sublimation at 298.15 K was obtained. By thermogravimetric data, it was possible to determine the molar enthalpy of sublimation and the molar enthalpy of vaporization. On the other hand, the molar enthalpy of formation in solid phase at 298.15 K was determined by combustion calorimetry. The molar enthalpy of formation in gas phase at 298.15 K was calculated from molar enthalpy of sublimation at 298.15 K and molar enthalpy of formation in solid phase at 298.15 K. This experimental value was compared both theoretical enthalpy of formation in gas phase by using Gaussian methods at the G3 and G4 levels and with an estimated value in relation to phthalic anhydride.

Abstract The present work aimed at evaluating the microstructures and mechanical properties of spark plasma sintered WC-6 wt% Co ceramic composites doped with boron (0.2 and 0.5 wt%). W-50 at% C (W-6.13 wt% C) powders produced under different milling times (20, 60, 180, and 600 min) were used as starting materials and subsequently mixed with Co (6 wt%) and B (0.2 or 0.5 wt%) powders for 5 min. The resultant WC-Co-B powder mixtures were consolidated by spark plasma sintering at 1450 °C for 10 min at 40 MPa under vacuum in order to obtain the samples with 10 mm diameter and 3 mm thickness. The starting powders and the sintered samples were characterized by laser diffraction particle size analysis, X-ray diffraction with Rietveld refinement, relative density, scanning electron microscopy, energy dispersive spectrometry, Vickers hardness test, and diametral compression test. Supersaturated W solid solution (WSS) with a crystallite size of <50 nm was formed in W-50C (at%) powder mixtures. The relative density of the sintered composites increased with the increasing milling time up to 180 min from 79.7%±0.6% to 86.7%±0.5% for WC-6Co-0.2B and from 86.7%±0.6% to 90.9%±0.4% for WC-6Co-0.5B. Furthermore, the WC phase appeared as the matrix in the sintered samples, and W2CoB2 precipitated homogeneously around WC grain boundaries, mainly to those containing 0.5% B. The Vickers hardness values of the sintered WC-6Co-0.2B and WC-6Co-0.5B ranged from 1790±39 to 2158±25 HV and 1858±31 to 2182±28 HV, respectively. The fracture strength (determined by diametral compression test) varied between 144-353 MPa due to the porosity and precipitates in thin-thickness cylinder samples.

The bone formation process is an example of how both time and nature acted harmonically to pave the path for life as we know. The unique association between calcium phosphate (CaP) and collagen in a single building block, gave rise to one of the most remarkable biomaterials that we know. Our aim in this revision is to provide a brief glance at the different organization levels of the bone tissue, by describing the main their main features. We start with a brief discussion regarding the thermodynamic conditions for mineral formation and how living organisms surpass them. Then we present collagen, the major organic component of bone, in the light of its hierarchical organization as well as its association to CaP crystals. The mechanisms that control both mineral formation and the association with the organic phase are further presented, in which cells and other components of the extracellular matrix, such as matrix vesicles and noncollagenous proteins, become agents of bone construction. Finally, we underline the difficulty of studying the complex process of bone mineralization and present liposomes, proteoliposomes, and Langmuir monolayers as biomimetic approaches to this end.