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au:QUEIROZ, ALINE C.
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1.
Factors associated with the completion of syphilis treatment among transgender women and travestis, in five Brazilian capitals, 2019-2021: a multicenter cross-sectional study
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Carreira, Luis Fernando Gomes
; Veras, Maria A. S.
; Benzaken, Adele Schwartz
; Queiroz, Rita Suely Bacuri de
; Silveira, Edilene Peres Real
; Oliveira, Elaine Lopes de
; Bassichetto, Katia Cristina
; Rocha, Aline Borges Moreira da
; Suprasert, Bow
; Wilson, Erin C.
; McFarland, Willi
.
RESUMO Objetivo Avaliar a história pregressa de sífilis em mulheres trans e travestis (MTTr) e identificar os fatores associados à incompletude do tratamento. Métodos Estudo transversal multicêntrico, entre 2019 e 2021, com participantes recrutadas por respondent-driven sampling, em cinco capitais brasileiras. Variável dependente: “tratamento referido de sífilis no último ano”, “não/incompleto” ou “completo”. Modelo multivariável-logístico foi utilizado para identificar fatores associados à completude. Resultados Das 1.317 participantes, 16,0% relataram sífilis pregressa. Dessas, 68,9% eram negras, 54,6% ganhavam até 1 salário mínimo e 61,1% completaram o tratamento. A completude de tratamento foi mais baixa em São Paulo (42,7%) e para quem sofreu agressão verbal (53,6%; ORa 0,46; IC95% 0,25;0,85). Conclusão Na amostra, foram elevadas: a prevalência de sífilis autorreferida e a proporção de participantes que refere não ter iniciado/completado o tratamento. É necessário identificar as barreiras enfrentadas por MTTr que impactam o acesso à saúde, e identificar suas necessidades, para assegurar diagnóstico e tratamento adequados.
ABSTRACT Objective To assess the previous history of syphilis in transgender women and travestis (TWTs) and identify factors associated with treatment incompleteness. Methods : This was a multicenter cross-sectional study conducted between 2019 and 2021, with participants recruited through respondent-driven sampling, in five Brazilian capitals. Dependent variable: “reported syphilis treatment in the last year”, “no/incomplete” or “complete”. A multivariate-logistic model was used to identify factors associated with completeness. Results : Of the 1,317 participants, 16.0% reported previous history of syphilis. Of these, 68.9% were Black, 54.6% earned up to 1 minimum wage and 61.1% completed the treatment. Treatment completion was lower in São Paulo (42.7%) and among those who experienced verbal abuse (53.6%; ORa 0.46; 95%CI 0.25;0.85). Conclusion In this sample, both the prevalence of self-reported syphilis and the proportion of participants who reported not having started/completed treatment were high. It is essential to identify the barriers faced by TWTs that hinder healthcare access, and identify their needs in order to ensure adequate diagnosis and treatment.
RESUMEN Objetivo Evaluar historia previa de sífilis en mujeres transgénero y travestis (MTT), identificar factores asociados al tratamiento incompleto. Métodos Estudio transversal multicéntrico, entre 2019-2021, con participantes reclutadas por Respondent-Driven Sampling, en cinco capitales brasileñas. Variable dependiente: “tratamiento de sífilis reportado el último año”: ‘no/incompleto’ o ‘completo’. Se utilizó un modelo logístico multivariable para identificar factores asociados a la incompletitud. Resultados 1.317 participantes, 16,0% refirieron sífilis previa. De estas 68,9% eran negras y 54,6% ganaban hasta 1 salario mínimo; 61,1% completaron tratamiento. La finalización del tratamiento fue menor en San Pablo (42,7%) y entre las que sufrieron agresión verbal (53,6%; ORa 0,46; IC95% 0,25;0,85). Conclusión Tanto prevalencia de sífilis autorreferida como proporción de participantes que informaron no haber iniciado/finalizado el tratamiento fueron elevadas. Es necesario identificar barreras enfrentadas por MTT que impactan su acceso a atención de salud, además, identificar sus necesidades específicas para garantizar un diagnóstico y tratamiento adecuados.
2.
In vitro evaluation against Leishmania amazonensis and Leishmania chagasi of medicinal plant species of interest to the Unified Health System
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ALBUQUERQUE, LILYANA WALESKA N.
; FERREIRA, SHAKIRA C.A.
; NUNES, IZABELLY CAROLLYNNY M.
; SANTOS, HILDA CAROLINE N.
; SANTOS, MARIANA S.
; VARJÃO, MÁRCIO THOMAZ S.
; SILVA, AMANDA EVELYN DA
; LEITE, ANDERSON B.
; DUARTE, ALYSSON W.F.
; ALEXANDRE-MOREIRA, MAGNA SUZANA
; QUEIROZ, ALINE C. DE
.
Anais da Academia Brasileira de Ciências
- Métricas do periódico
Abstract Leishmaniasis is a disease of public health relevance that demands new therapeutic alternatives due to the toxicity of conventional treatments. In this study, 27 plants of interest to the Unified Health System (SUS) were evaluated for cytotoxicity in macrophages, leishmanicidal activity and production of nitric oxide (NO). None of the species demonstrated cytotoxicity to macrophages (CC50 >100 μg/mL). Extracts from Chenopodium ambrosioides, Equisetum arvense, Maytenus ilicifolia showed greater efficacy in inducing the death of Leishmania amazonensis amastigotes with IC50 of 68.4, 82.3, 75.7 μg/mL, respectively. The species Cynara scolymus, Punica granatum and Passiflora alata were the most effective in inducing an increase in the indirect concentration of NO (41.31, 29.30 and 28.86 µM, respectively) in cultures of macrophages infected with L. amazonensis. Furthermore, Punica granatum was also the most effective species in inducing an increase in NO in macrophages infected by Leishmania chagasi (19.90 µM). The results obtained so far support the continuation of studies, with the possibility of developing safer and more effective treatments for leishmaniasis, using natural products. The identification of plants that stimulate the production of NO in macrophages infected by Leishmania opens doors for more detailed investigations of the mechanism of action of these natural products. study 2 SUS (SUS NO. . (NO) CC50 CC (CC5 100 >10 μg/mL. μgmL μg/mL μg mL μg/mL) ambrosioides arvense IC IC5 684 68 4 68.4 823 82 3 82.3 757 75 7 75. respectively scolymus 41.31, 4131 41 31 (41.31 2930 29 30 29.3 2886 28 86 28.8 µM L Furthermore 19.90 1990 19 90 (19.9 µM. µM) studies leishmaniasis products (NO CC5 (CC 10 >1 6 68. 8 82. 41.31 413 (41.3 293 29. 288 28. 19.9 199 1 9 (19. > 41.3 (41. 19. (19 41. (41 (1 (4 (
3.
Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil Brasil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
; Slipinski, Adam
; Linzmeier, Adelita M.
; Calor, Adolfo R.
; Garda, Adrian A.
; Kury, Adriano B.
; Fernandes, Agatha C.S.
; Agudo-Padrón, Aisur I.
; Akama, Alberto
; Silva Neto, Alberto M. da
; Burbano, Alejandro L.
; Menezes, Aleksandra
; Pereira-Colavite, Alessandre
; Anichtchenko, Alexander
; Lees, Alexander C.
; Bezerra, Alexandra M.R.
; Domahovski, Alexandre C.
; Pimenta, Alexandre D.
; Aleixo, Alexandre L.P.
; Marceniuk, Alexandre P.
; Paula, Alexandre S. de
; Somavilla, Alexandre
; Specht, Alexandre
; Camargo, Alexssandro
; Newton, Alfred F.
; Silva, Aline A.S. da
; Santos, Aline B. dos
; Tassi, Aline D.
; Aragão, Allan C.
; Santos, Allan P.M.
; Migotto, Alvaro E.
; Mendes, Amanda C.
; Cunha, Amanda
; Chagas Júnior, Amazonas
; Sousa, Ana A.T. de
; Pavan, Ana C.
; Almeida, Ana C.S.
; Peronti, Ana L.B.G.
; Henriques-Oliveira, Ana L.
; Prudente, Ana L.
; Tourinho, Ana L.
; Pes, Ana M.O.
; Carmignotto, Ana P.
; Wengrat, Ana P.G. da Silva
; Dornellas, Ana P.S.
; Molin, Anamaria Dal
; Puker, Anderson
; Morandini, André C.
; Ferreira, André da S.
; Martins, André L.
; Esteves, André M.
; Fernandes, André S.
; Roza, André S.
; Köhler, Andreas
; Paladini, Andressa
; Andrade, Andrey J. de
; Pinto, Ângelo P.
; Salles, Anna C. de A.
; Gondim, Anne I.
; Amaral, Antonia C.Z.
; Rondón, Antonio A.A.
; Brescovit, Antonio
; Lofego, Antônio C.
; Marques, Antonio C.
; Macedo, Antonio
; Andriolo, Artur
; Henriques, Augusto L.
; Ferreira Júnior, Augusto L.
; Lima, Aurino F. de
; Barros, Ávyla R. de A.
; Brito, Ayrton do R.
; Romera, Bárbara L.V.
; Vasconcelos, Beatriz M.C. de
; Frable, Benjamin W.
; Santos, Bernardo F.
; Ferraz, Bernardo R.
; Rosa, Brunno B.
; Sampaio, Brunno H.L.
; Bellini, Bruno C.
; Clarkson, Bruno
; Oliveira, Bruno G. de
; Corrêa, Caio C.D.
; Martins, Caleb C.
; Castro-Guedes, Camila F. de
; Souto, Camilla
; Bicho, Carla de L.
; Cunha, Carlo M.
; Barboza, Carlos A. de M.
; Lucena, Carlos A.S. de
; Barreto, Carlos
; Santana, Carlos D.C.M. de
; Agne, Carlos E.Q.
; Mielke, Carlos G.C.
; Caetano, Carlos H.S.
; Flechtmann, Carlos H.W.
; Lamas, Carlos J.E.
; Rocha, Carlos
; Mascarenhas, Carolina S.
; Margaría, Cecilia B.
; Waichert, Cecilia
; Digiani, Celina
; Haddad, Célio F.B.
; Azevedo, Celso O.
; Benetti, Cesar J.
; Santos, Charles M.D. dos
; Bartlett, Charles R.
; Bonvicino, Cibele
; Ribeiro-Costa, Cibele S.
; Santos, Cinthya S.G.
; Justino, Cíntia E.L.
; Canedo, Clarissa
; Bonecker, Claudia C.
; Santos, Cláudia P.
; Carvalho, Claudio J.B. de
; Gonçalves, Clayton C.
; Galvão, Cleber
; Costa, Cleide
; Oliveira, Cléo D.C. de
; Schwertner, Cristiano F.
; Andrade, Cristiano L.
; Pereira, Cristiano M.
; Sampaio, Cristiano
; Dias, Cristina de O.
; Lucena, Daercio A. de A.
; Manfio, Daiara
; Amorim, Dalton de S.
; Queiroz, Dalva L. de
; Queiroz, Dalva L. de
; Colpani, Daniara
; Abbate, Daniel
; Aquino, Daniel A.
; Burckhardt, Daniel
; Cavallari, Daniel C.
; Prado, Daniel de C. Schelesky
; Praciano, Daniel L.
; Basílio, Daniel S.
; Bená, Daniela de C.
; Toledo, Daniela G.P. de
; Takiya, Daniela M.
; Fernandes, Daniell R.R.
; Ament, Danilo C.
; Cordeiro, Danilo P.
; Silva, Darliane E.
; Pollock, Darren A.
; Muniz, David B.
; Gibson, David I.
; Nogueira, David S.
; Marques, Dayse W.A.
; Lucatelli, Débora
; Garcia, Deivys M.A.
; Baêta, Délio
; Ferreira, Denise N.M.
; Rueda-Ramírez, Diana
; Fachin, Diego A.
; Souza, Diego de S.
; Rodrigues, Diego F.
; Pádua, Diego G. de
; Barbosa, Diego N.
; Dolibaina, Diego R.
; Amaral, Diogo C.
; Chandler, Donald S.
; Maccagnan, Douglas H.B.
; Caron, Edilson
; Carvalho, Edrielly
; Adriano, Edson A.
; Abreu Júnior, Edson F. de
; Pereira, Edson H.L.
; Viegas, Eduarda F.G.
; Carneiro, Eduardo
; Colley, Eduardo
; Eizirik, Eduardo
; Santos, Eduardo F. dos
; Shimbori, Eduardo M.
; Suárez-Morales, Eduardo
; Arruda, Eliane P. de
; Chiquito, Elisandra A.
; Lima, Élison F.B.
; Castro, Elizeu B. de
; Orlandin, Elton
; Nascimento, Elynton A. do
; Razzolini, Emanuel
; Gama, Emanuel R.R.
; Araujo, Enilma M. de
; Nishiyama, Eric Y.
; Spiessberger, Erich L.
; Santos, Érika C.L. dos
; Contreras, Eugenia F.
; Galati, Eunice A.B.
; Oliveira Junior, Evaldo C. de
; Gallardo, Fabiana
; Hernandes, Fabio A.
; Lansac-Tôha, Fábio A.
; Pitombo, Fabio B.
; Dario, Fabio Di
; Santos, Fábio L. dos
; Mauro, Fabio
; Nascimento, Fabio O. do
; Olmos, Fabio
; Amaral, Fabio R.
; Schunck, Fabio
; Godoi, Fábio S. P. de
; Machado, Fabrizio M.
; Barbo, Fausto E.
; Agrain, Federico A.
; Ribeiro, Felipe B.
; Moreira, Felipe F.F.
; Barbosa, Felipe F.
; Silva, Fenanda S.
; Cavalcanti, Fernanda F.
; Straube, Fernando C.
; Carbayo, Fernando
; Carvalho Filho, Fernando
; Zanella, Fernando C.V.
; Jacinavicius, Fernando de C.
; Farache, Fernando H.A.
; Leivas, Fernando
; Dias, Fernando M.S.
; Mantellato, Fernando
; Vaz-de-Mello, Fernando Z.
; Gudin, Filipe M.
; Albuquerque, Flávio
; Molina, Flavio B.
; Passos, Flávio D.
; Shockley, Floyd W.
; Pinheiro, Francielly F.
; Mello, Francisco de A.G. de
; Nascimento, Francisco E. de L.
; Franco, Francisco L.
; Oliveira, Francisco L. de
; Melo, Francisco T. de V.
; Quijano, Freddy R.B.
; Salles, Frederico F.
; Biffi, Gabriel
; Queiroz, Gabriel C.
; Bizarro, Gabriel L.
; Hrycyna, Gabriela
; Leviski, Gabriela
; Powell, Gareth S.
; Santos, Geane B. dos
; Morse, Geoffrey E.
; Brown, George
; Mattox, George M.T.
; Zimbrão, Geraldo
; Carvalho, Gervásio S.
; Miranda, Gil F.G.
; Moraes, Gilberto J. de
; Lourido, Gilcélia M.
; Neves, Gilmar P.
; Moreira, Gilson R.P.
; Montingelli, Giovanna G.
; Maurício, Giovanni N.
; Marconato, Gláucia
; Lopez, Guilherme E.L.
; Silva, Guilherme L. da
; Muricy, Guilherme
; Brito, Guilherme R.R.
; Garbino, Guilherme S.T.
; Flores, Gustavo E.
; Graciolli, Gustavo
; Libardi, Gustavo S.
; Proctor, Heather C.
; Gil-Santana, Helcio R.
; Varella, Henrique R.
; Escalona, Hermes E.
; Schmitz, Hermes J.
; Rodrigues, Higor D.D.
; Galvão Filho, Hilton de C.
; Quintino, Hingrid Y.S.
; Pinto, Hudson A.
; Rainho, Hugo L.
; Miyahira, Igor C.
; Gonçalves, Igor de S.
; Martins, Inês X.
; Cardoso, Irene A.
; Oliveira, Ismael B. de
; Franz, Ismael
; Fernandes, Itanna O.
; Golfetti, Ivan F.
; S. Campos-Filho, Ivanklin
; Oliveira, Ivo de S.
; Delabie, Jacques H.C.
; Oliveira, Jader de
; Prando, Jadila S.
; Patton, James L.
; Bitencourt, Jamille de A.
; Silva, Janaina M.
; Santos, Jandir C.
; Arruda, Janine O.
; Valderrama, Jefferson S.
; Dalapicolla, Jeronymo
; Oliveira, Jéssica P.
; Hájek, Jiri
; Morselli, João P.
; Narita, João P.
; Martin, João P.I.
; Grazia, Jocélia
; McHugh, Joe
; Cherem, Jorge J.
; Farias Júnior, José A.S.
; Fernandes, Jose A.M.
; Pacheco, José F.
; Birindelli, José L.O.
; Rezende, José M.
; Avendaño, Jose M.
; Duarte, José M. Barbanti
; Ribeiro, José R. Inácio
; Mermudes, José R.M.
; Pujol-Luz, José R.
; Santos, Josenilson R. dos
; Câmara, Josenir T.
; Teixeira, Joyce A.
; Prado, Joyce R. do
; Botero, Juan P.
; Almeida, Julia C.
; Kohler, Julia
; Gonçalves, Julia P.
; Beneti, Julia S.
; Donahue, Julian P.
; Alvim, Juliana
; Almeida, Juliana C.
; Segadilha, Juliana L.
; Wingert, Juliana M.
; Barbosa, Julianna F.
; Ferrer, Juliano
; Santos, Juliano F. dos
; Kuabara, Kamila M.D.
; Nascimento, Karine B.
; Schoeninger, Karine
; Campião, Karla M.
; Soares, Karla
; Zilch, Kássia
; Barão, Kim R.
; Teixeira, Larissa
; Sousa, Laura D. do N.M. de
; Dumas, Leandro L.
; Vieira, Leandro M.
; Azevedo, Leonardo H.G.
; Carvalho, Leonardo S.
; Souza, Leonardo S. de
; Rocha, Leonardo S.G.
; Bernardi, Leopoldo F.O.
; Vieira, Letícia M.
; Johann, Liana
; Salvatierra, Lidianne
; Oliveira, Livia de M.
; Loureiro, Lourdes M.A. El-moor
; Barreto, Luana B.
; Barros, Luana M.
; Lecci, Lucas
; Camargos, Lucas M. de
; Lima, Lucas R.C.
; Almeida, Lucia M.
; Martins, Luciana R.
; Marinoni, Luciane
; Moura, Luciano de A.
; Lima, Luciano
; Naka, Luciano N.
; Miranda, Lucília S.
; Salik, Lucy M.
; Bezerra, Luis E.A.
; Silveira, Luis F.
; Campos, Luiz A.
; Castro, Luiz A.S. de
; Pinho, Luiz C.
; Silveira, Luiz F.L.
; Iniesta, Luiz F.M.
; Tencatt, Luiz F.C.
; Simone, Luiz R.L.
; Malabarba, Luiz R.
; Cruz, Luiza S. da
; Sekerka, Lukas
; Barros, Lurdiana D.
; Santos, Luziany Q.
; Skoracki, Maciej
; Correia, Maira A.
; Uchoa, Manoel A.
; Andrade, Manuella F.G.
; Hermes, Marcel G.
; Miranda, Marcel S.
; Araújo, Marcel S. de
; Monné, Marcela L.
; Labruna, Marcelo B.
; Santis, Marcelo D. de
; Duarte, Marcelo
; Knoff, Marcelo
; Nogueira, Marcelo
; Britto, Marcelo R. de
; Melo, Marcelo R.S. de
; Carvalho, Marcelo R. de
; Tavares, Marcelo T.
; Kitahara, Marcelo V.
; Justo, Marcia C.N.
; Botelho, Marcia J.C.
; Couri, Márcia S.
; Borges-Martins, Márcio
; Felix, Márcio
; Oliveira, Marcio L. de
; Bologna, Marco A.
; Gottschalk, Marco S.
; Tavares, Marcos D.S.
; Lhano, Marcos G.
; Bevilaqua, Marcus
; Santos, Marcus T.T.
; Domingues, Marcus V.
; Sallum, Maria A.M.
; Digiani, María C.
; Santarém, Maria C.A.
; Nascimento, Maria C. do
; Becerril, María de los A.M.
; Santos, Maria E.A. dos
; Passos, Maria I. da S. dos
; Felippe-Bauer, Maria L.
; Cherman, Mariana A.
; Terossi, Mariana
; Bartz, Marie L.C.
; Barbosa, Marina F. de C.
; Loeb, Marina V.
; Cohn-Haft, Mario
; Cupello, Mario
; Martins, Marlúcia B.
; Christofersen, Martin L.
; Bento, Matheus
; Rocha, Matheus dos S.
; Martins, Maurício L.
; Segura, Melissa O.
; Cardenas, Melissa Q.
; Duarte, Mércia E.
; Ivie, Michael A.
; Mincarone, Michael M.
; Borges, Michela
; Monné, Miguel A.
; Casagrande, Mirna M.
; Fernandez, Monica A.
; Piovesan, Mônica
; Menezes, Naércio A.
; Benaim, Natalia P.
; Reategui, Natália S.
; Pedro, Natan C.
; Pecly, Nathalia H.
; Ferreira Júnior, Nelson
; Silva Júnior, Nelson J. da
; Perioto, Nelson W.
; Hamada, Neusa
; Degallier, Nicolas
; Chao, Ning L.
; Ferla, Noeli J.
; Mielke, Olaf H.H.
; Evangelista, Olivia
; Shibatta, Oscar A.
; Oliveira, Otto M.P.
; Albornoz, Pablo C.L.
; Dellapé, Pablo M.
; Gonçalves, Pablo R.
; Shimabukuro, Paloma H.F.
; Grossi, Paschoal
; Rodrigues, Patrícia E. da S.
; Lima, Patricia O.V.
; Velazco, Paul
; Santos, Paula B. dos
; Araújo, Paula B.
; Silva, Paula K.R.
; Riccardi, Paula R.
; Garcia, Paulo C. de A.
; Passos, Paulo G.H.
; Corgosinho, Paulo H.C.
; Lucinda, Paulo
; Costa, Paulo M.S.
; Alves, Paulo P.
; Roth, Paulo R. de O.
; Coelho, Paulo R.S.
; Duarte, Paulo R.M.
; Carvalho, Pedro F. de
; Gnaspini, Pedro
; Souza-Dias, Pedro G.B.
; Linardi, Pedro M.
; Bartholomay, Pedro R.
; Demite, Peterson R.
; Bulirsch, Petr
; Boll, Piter K.
; Pereira, Rachel M.M.
; Silva, Rafael A.P.F.
; Moura, Rafael B. de
; Boldrini, Rafael
; Silva, Rafaela A. da
; Falaschi, Rafaela L.
; Cordeiro, Ralf T.S.
; Mello, Ramon J.C.L.
; Singer, Randal A.
; Querino, Ranyse B.
; Heleodoro, Raphael A.
; Castilho, Raphael de C.
; Constantino, Reginaldo
; Guedes, Reinaldo C.
; Carrenho, Renan
; Gomes, Renata S.
; Gregorin, Renato
; Machado, Renato J.P.
; Bérnils, Renato S.
; Capellari, Renato S.
; Silva, Ricardo B.
; Kawada, Ricardo
; Dias, Ricardo M.
; Siewert, Ricardo
; Brugnera, Ricaro
; Leschen, Richard A.B.
; Constantin, Robert
; Robbins, Robert
; Pinto, Roberta R.
; Reis, Roberto E. dos
; Ramos, Robson T. da C.
; Cavichioli, Rodney R.
; Barros, Rodolfo C. de
; Caires, Rodrigo A.
; Salvador, Rodrigo B.
; Marques, Rodrigo C.
; Araújo, Rodrigo C.
; Araujo, Rodrigo de O.
; Dios, Rodrigo de V.P.
; Johnsson, Rodrigo
; Feitosa, Rodrigo M.
; Hutchings, Roger W.
; Lara, Rogéria I.R.
; Rossi, Rogério V.
; Gerstmeier, Roland
; Ochoa, Ronald
; Hutchings, Rosa S.G.
; Ale-Rocha, Rosaly
; Rocha, Rosana M. da
; Tidon, Rosana
; Brito, Rosangela
; Pellens, Roseli
; Santos, Sabrina R. dos
; Santos, Sandra D. dos
; Paiva, Sandra V.
; Santos, Sandro
; Oliveira, Sarah S. de
; Costa, Sávio C.
; Gardner, Scott L.
; Leal, Sebastián A. Muñoz
; Aloquio, Sergio
; Bonecker, Sergio L.C.
; Bueno, Sergio L. de S.
; Almeida, Sérgio M. de
; Stampar, Sérgio N.
; Andena, Sérgio R.
; Posso, Sergio R.
; Lima, Sheila P.
; Gadelha, Sian de S.
; Thiengo, Silvana C.
; Cohen, Simone C.
; Brandão, Simone N.
; Rosa, Simone P.
; Ribeiro, Síria L.B.
; Letana, Sócrates D.
; Santos, Sonia B. dos
; Andrade, Sonia C.S.
; Dávila, Stephane
; Vaz, Stéphanie
; Peck, Stewart B.
; Christo, Susete W.
; Cunha, Suzan B.Z.
; Gomes, Suzete R.
; Duarte, Tácio
; Madeira-Ott, Taís
; Marques, Taísa
; Roell, Talita
; Lima, Tarcilla C. de
; Sepulveda, Tatiana A.
; Maria, Tatiana F.
; Ruschel, Tatiana P.
; Rodrigues, Thaiana
; Marinho, Thais A.
; Almeida, Thaís M. de
; Miranda, Thaís P.
; Freitas, Thales R.O.
; Pereira, Thalles P.L.
; Zacca, Thamara
; Pacheco, Thaynara L.
; Martins, Thiago F.
; Alvarenga, Thiago M.
; Carvalho, Thiago R. de
; Polizei, Thiago T.S.
; McElrath, Thomas C.
; Henry, Thomas
; Pikart, Tiago G.
; Porto, Tiago J.
; Krolow, Tiago K.
; Carvalho, Tiago P.
; Lotufo, Tito M. da C.
; Caramaschi, Ulisses
; Pinheiro, Ulisses dos S.
; Pardiñas, Ulyses F.J.
; Maia, Valéria C.
; Tavares, Valeria
; Costa, Valmir A.
; Amaral, Vanessa S. do
; Silva, Vera C.
; Wolff, Vera R. dos S.
; Slobodian, Verônica
; Silva, Vinícius B. da
; Espíndola, Vinicius C.
; Costa-Silva, Vinicius da
; Bertaco, Vinicius de A.
; Padula, Vinícius
; Ferreira, Vinicius S.
; Silva, Vitor C.P. da
; Piacentini, Vítor de Q.
; Sandoval-Gómez, Vivian E.
; Trevine, Vivian
; Sousa, Viviane R.
; Sant’Anna, Vivianne B. de
; Mathis, Wayne N.
; Souza, Wesley de O.
; Colombo, Wesley D.
; Tomaszewska, Wioletta
; Wosiacki, Wolmar B.
; Ovando, Ximena M.C.
; Leite, Yuri L.R.
.
ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others. publications problem uptodate up date classifications context exception (CTFB http//fauna.jbrj.gov.br/, httpfaunajbrjgovbr http //fauna.jbrj.gov.br/ , jbrj gov br (http://fauna.jbrj.gov.br/) 2015 Brazil 80 specialists 1 2024 133691 133 691 133,69 125138 125 138 125,13 82.3%, 823 82 3 (82.3% 102000 102 000 102,00 7.69%, 769 7 69 (7.69% 11000 11 11,00 . 3,567 3567 567 (3,56 2,292 2292 2 292 (2,29 1,833 1833 833 (1,83 1,447 1447 447 (1,44 1000 1,00 831 (83 628 (62 606 (60 520 (52 50 users science health biology law anthropology education others http//fauna.jbrj.gov.br/ faunajbrjgovbr //fauna.jbrj.gov.br (http://fauna.jbrj.gov.br/ 201 8 202 13369 13 133,6 12513 12 125,1 82.3% (82.3 10200 10 00 102,0 7.69% 76 6 (7.69 1100 11,0 3,56 356 56 (3,5 2,29 229 29 (2,2 1,83 183 83 (1,8 1,44 144 44 (1,4 100 1,0 (8 62 (6 60 52 (5 5 http//fauna.jbrj.gov.br (http://fauna.jbrj.gov.br 20 1336 133, 1251 125, 82.3 (82. 1020 0 102, 7.69 (7.6 110 11, 3,5 35 (3, 2,2 22 (2, 1,8 18 (1, 1,4 14 4 ( 82. (82 7.6 (7. 3, (3 2, (2 (1 7. (7
4.
Anti-inflammatory and Antinociceptive Activities of Aqueous and Ethanolic Extracts from Cereus jamacaru DC. (Cactaceae) Antiinflammatory Anti inflammatory DC Cactaceae (Cactaceae
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Lima, Alyne Almeida de
; Viana, Max Denisson Maurício
; Silva Neto, Geraldo José da
; Silva, Suellen Maria Albuquerque da
; Bezerra, Pedro Henrique Simões
; Queiroz, Aline Cavalcanti de
; Moreira, Magna Suzana Alexandre
; Lauria, Pedro Santana Sales
; Campesatto, Eliane Aparecida
.
Brazilian Archives of Biology and Technology
- Métricas do periódico
Abstract Cereus jamacaru DC. (Cactaceae) is used in folk medicine for treating pain and inflammation. However, the therapeutic potential of C. jamacaru extracts has not been assessed experimentally. This study aimed to characterize the effect of both aqueous (CAECJ) and ethanolic (CEECJ) extracts of C. jamacaru in mouse models of pain and inflammation. CAECJ and CEECJ were chemically characterized by qualitative methods. Toxicity of extracts was evaluated in vitro against J774.A1 macrophages and in vivo by monitoring mice for 14 days following a single oral treatment at 2000 mg/kg. The antinociceptive activity of the extracts was assessed in models of pain in mice: acetic acid-induced writhing test, formalin test, hot plate test, and glutamate-induced nociception assay. The anti-inflammatory activity of the extracts was assessed following zymosan-induced peritonitis and in the model of arthritis induced by Complete Freund’s Adjuvant (CFA). Phytochemical analyses revealed alkaloids, condensed tannins, flavonoids, and anthraquinones in both extracts; saponins were present in CEECJ only. Neither extract was cytotoxic in vitro or induced toxicity in mice. Both extracts reduced nociception in all models of nociception without impairing motor function. Both extracts reduced leucocyte migration during experimental peritonitis and reduced paw edema during experimental arthritis. The effects of the extracts can be both due to anti-inflammatory and centrally-mediated mechanisms. This paper corroborates the traditional use of C. jamacaru extracts and demonstrates their therapeutic potential in the development of new analgesic and anti-inflammatory drugs with a good safety profile. DC Cactaceae (Cactaceae inflammation However C experimentally (CAECJ (CEECJ methods J774A1 JA J774 A1 J A J774.A 1 200 mgkg mg kg mg/kg acidinduced acid test glutamateinduced glutamate assay antiinflammatory anti inflammatory zymosaninduced zymosan Freunds Freund s CFA. CFA . (CFA) alkaloids tannins flavonoids only function centrallymediated centrally mediated mechanisms profile J774A J77 20 (CFA J7 2
5.
Factors associated with the completion of syphilis treatment among transgender women and travestis, in five Brazilian capitals, 2019-2021: a multicenter cross-sectional study travestis capitals 20192021 2019 2021 2019-2021 crosssectional cross sectional 2019202 201 202 2019-202 201920 20 2019-20 20192 2 2019-2 2019-
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Carreira, Luis Fernando Gomes
; Veras, Maria A. S.
; Benzaken, Adele Schwartz
; Queiroz, Rita Suely Bacuri de
; Silveira, Edilene Peres Real
; Oliveira, Elaine Lopes de
; Bassichetto, Katia Cristina
; Rocha, Aline Borges Moreira da
; Suprasert, Bow
; Wilson, Erin C.
; McFarland, Willi
.
RESUMO Objetivo Avaliar a história pregressa de sífilis em mulheres trans e travestis (MTTr) e identificar os fatores associados à incompletude do tratamento. Métodos Estudo transversal multicêntrico, entre 2019 e 2021, com participantes recrutadas por respondent-driven sampling, em cinco capitais brasileiras. Variável dependente: “tratamento referido de sífilis no último ano”, “não/incompleto” ou “completo”. Modelo multivariável-logístico foi utilizado para identificar fatores associados à completude. Resultados Das 1.317 participantes, 16,0% relataram sífilis pregressa. Dessas, 68,9% eram negras, 54,6% ganhavam até 1 salário mínimo e 61,1% completaram o tratamento. A completude de tratamento foi mais baixa em São Paulo (42,7%) e para quem sofreu agressão verbal (53,6%; ORa 0,46; IC95% 0,25;0,85). Conclusão Na amostra, foram elevadas: a prevalência de sífilis autorreferida e a proporção de participantes que refere não ter iniciado/completado o tratamento. É necessário identificar as barreiras enfrentadas por MTTr que impactam o acesso à saúde, e identificar suas necessidades, para assegurar diagnóstico e tratamento adequados. (MTTr multicêntrico 201 2021 respondentdriven respondent driven sampling brasileiras dependente ano, ano , ano” não/incompleto nãoincompleto incompleto “não/incompleto completo. completo . “completo” multivariávellogístico multivariável logístico 1317 317 1.31 160 16 0 16,0 Dessas 689 68 9 68,9 negras 546 54 6 54,6 611 61 61,1 42,7% 427 42 7 (42,7% 53,6% 536 53 (53,6% 0,46 046 46 IC95 IC 0,250,85. 025085 0,25 0,85 25 85 0,25;0,85) amostra elevadas iniciadocompletado iniciado completado saúde necessidades adequados 20 202 “completo 131 31 1.3 16, 68, 5 54, 61, 42,7 4 (42,7 53,6 (53,6 0,4 04 IC9 250 0,250,85 02508 025 0,2 085 0,8 2 8 0,25;0,85 13 3 1. 42, (42, 53, (53, 0, 0,250,8 0250 02 08 0,25;0,8 (42 (53 0,250, 0,25;0, (4 (5 0,250 0,25;0 ( 0,25;
ABSTRACT Objective To assess the previous history of syphilis in transgender women and travestis (TWTs) and identify factors associated with treatment incompleteness. Methods : This was a multicenter cross-sectional study conducted between 2019 and 2021, with participants recruited through respondent-driven sampling, in five Brazilian capitals. Dependent variable: “reported syphilis treatment in the last year”, “no/incomplete” or “complete”. A multivariate-logistic model was used to identify factors associated with completeness. Results : Of the 1,317 participants, 16.0% reported previous history of syphilis. Of these, 68.9% were Black, 54.6% earned up to 1 minimum wage and 61.1% completed the treatment. Treatment completion was lower in São Paulo (42.7%) and among those who experienced verbal abuse (53.6%; ORa 0.46; 95%CI 0.25;0.85). Conclusion In this sample, both the prevalence of self-reported syphilis and the proportion of participants who reported not having started/completed treatment were high. It is essential to identify the barriers faced by TWTs that hinder healthcare access, and identify their needs in order to ensure adequate diagnosis and treatment. (TWTs incompleteness crosssectional cross sectional 201 2021 respondentdriven respondent driven sampling capitals variable year, year , year” no/incomplete noincomplete no incomplete “no/incomplete complete. complete . “complete” multivariatelogistic multivariate logistic completeness 1317 317 1,31 160 16 0 16.0 these 689 68 9 68.9 Black 546 54 6 54.6 611 61 61.1 42.7% 427 42 7 (42.7% 53.6% 536 53 (53.6% 0.46 046 46 95CI CI 95 0.250.85. 025085 0.25 0.85 25 85 0.25;0.85) sample selfreported self startedcompleted started high access 20 202 “complete 131 31 1,3 16. 68. 5 54. 61. 42.7 4 (42.7 53.6 (53.6 0.4 04 250 0.250.85 02508 025 0.2 085 0.8 2 8 0.25;0.85 13 3 1, 42. (42. 53. (53. 0. 0.250.8 0250 02 08 0.25;0.8 (42 (53 0.250. 0.25;0. (4 (5 0.250 0.25;0 ( 0.25;
RESUMEN Objetivo Evaluar historia previa de sífilis en mujeres transgénero y travestis (MTT), identificar factores asociados al tratamiento incompleto. Métodos Estudio transversal multicéntrico, entre 2019-2021, con participantes reclutadas por Respondent-Driven Sampling, en cinco capitales brasileñas. Variable dependiente: “tratamiento de sífilis reportado el último año”: ‘no/incompleto’ o ‘completo’. Se utilizó un modelo logístico multivariable para identificar factores asociados a la incompletitud. Resultados 1.317 participantes, 16,0% refirieron sífilis previa. De estas 68,9% eran negras y 54,6% ganaban hasta 1 salario mínimo; 61,1% completaron tratamiento. La finalización del tratamiento fue menor en San Pablo (42,7%) y entre las que sufrieron agresión verbal (53,6%; ORa 0,46; IC95% 0,25;0,85). Conclusión Tanto prevalencia de sífilis autorreferida como proporción de participantes que informaron no haber iniciado/finalizado el tratamiento fueron elevadas. Es necesario identificar barreras enfrentadas por MTT que impactan su acceso a atención de salud, además, identificar sus necesidades específicas para garantizar un diagnóstico y tratamiento adecuados. MTT, , (MTT) incompleto multicéntrico 20192021, 20192021 2019 2021, 2021 2019-2021 RespondentDriven Respondent Driven Sampling brasileñas dependiente año año” ‘no/incompleto noincompleto ‘completo. completo ‘completo . ‘completo’ incompletitud 1317 317 1.31 160 16 0 16,0 689 68 9 68,9 546 54 6 54,6 mínimo 611 61 61,1 42,7% 427 42 7 (42,7% 53,6% 536 53 (53,6% 0,46 046 46 IC95 IC 0,250,85. 025085 0,25 0,85 25 85 0,25;0,85) iniciadofinalizado iniciado finalizado elevadas salud además adecuados (MTT 2019202 201 202 2019-202 131 31 1.3 16, 68, 5 54, 61, 42,7 4 (42,7 53,6 (53,6 0,4 04 IC9 250 0,250,85 02508 025 0,2 085 0,8 2 8 0,25;0,85 201920 20 2019-20 13 3 1. 42, (42, 53, (53, 0, 0,250,8 0250 02 08 0,25;0,8 20192 2019-2 (42 (53 0,250, 0,25;0, 2019- (4 (5 0,250 0,25;0 ( 0,25;
6.
Differentials in death count records by databases in Brazil in 2010
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Diógenes, Victor Hugo Dias
; Pinto Júnior, Elzo Pereira
; Gonzaga, Marcos Roberto
; Queiroz, Bernardo Lanza
; Lima, Everton E. C.
; Costa, Lilia Carolina C. da
; Rocha, Aline S.
; Ferreira, Andrêa J. F.
; Teixeira, Camila S. S.
; Alves, Flávia Jôse O.
; Rameh, Leila
; Flores-Ortiz, Renzo
; Leyland, Alastair
; Dundas, Ruth
; Barreto, Maurício L.
; Ichihara, Maria Yury Travassos
.
ABSTRACT OBJECTIVE To compare the death counts from three sources of information on mortality available in Brazil in 2010, the Mortality Information System (SIM - Sistema de Informações sobre Mortalidade ), Civil Registration Statistic System (RC - Sistema de Estatísticas de Resgistro Civil ), and the 2010 Demographic Census at various geographical levels, and to confirm the association between municipal socioeconomic characteristics and the source which showed the highest death count. METHODS This is a descriptive and comparative study of raw data on deaths in the SIM, RC and 2010 Census databases, the latter held in Brazilian states and municipalities between August 2009 and July 2010. The percentage of municipalities was confirmed by the database showing the highest death count. The association between the source of the highest death count and socioeconomic indicators - the Índice de Privação Brasileiro (IBP – Brazilian Deprivation Index) and Índice de Desenvolvimento Humano Municipal (IHDM – Municipal Human Development Index) - was performed by bivariate choropleth and Moran Local Index of Spatial Association (LISA) cluster maps. RESULTS Confirmed that the SIM is the database with the highest number of deaths counted for all Brazilian macroregions, except the North, in which the highest coverage was from the 2010 Census. Based on the indicators proposed, in general, the Census showed a higher coverage of deaths than the SIM and the RC in the most deprived (highest IBP values) and less developed municipalities (lowest IDHM values) in the country. CONCLUSION The results highlight regional inequalities in how the databases chosen for this study cover death records, and the importance of maintaining the issue of mortality on the basic census questionnaire.
7.
Antarctic environments as a source of bacterial and fungal therapeutic enzymes
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LIMA, IGOR G.O.
; BISPO, JAMES R.S.
; AGOSTINHO, ADSON Y.H.
; QUEIROZ, ALINE C. DE
; MOREIRA, MAGNA SUZANA A.
; PASSARINI, MICHEL RODRIGO Z.
; OLIVEIRA, VALÉRIA M. DE
; SETTE, LARA D.
; ROSA, LUIZ HENRIQUE
; DUARTE, ALYSSON WAGNER F.
.
Anais da Academia Brasileira de Ciências
- Métricas do periódico
Abstract Microbial therapeutic enzymes are the protagonists in the pharmacological treatment of different human diseases. The intrinsic enzymatic characteristics, such as high affinity and specificity to the corresponding substrate, enable effective therapies, with minimal adverse effects and complete remission. However, immunogenicity, short half-life, low enzymatic yield, and low selectivity regarding available enzyme drugs are currently the main obstacles to their development and the broad adherence to therapeutic protocols. By harboring adapted and still unexplored microbial life, environments of extreme conditions, such as Antarctica, become especially important in the prospecting and development of new enzymatic compounds that present higher yields and the possibility of genetic improvement. Antarctic microorganisms have adaptation mechanisms, such as more fluid cell membranes, production of antifreeze proteins and enzymes with more malleable structures, more robust, stable, selective catalytic sites for their respective substrates, and high antioxidant capacity. In this context, this review aims to explore enzymes synthesized by bacteria and fungi from Antarctica as potential drug producers, capable of providing therapeutic efficacy, less adverse effects, and lower production costs with highlight to L-Asparaginase, collagenase, superoxide dismutase and ribonucleases. In addition, this review highlights the unique biotechnological profile of these Antarctic extremophile microorganisms.
8.
Extracellular hydrolytic enzymes produced by yeasts from Antarctic lichens
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DA SILVA, MAYANNE KARLA
; DA SILVA, AVERLANE V.
; FERNANDEZ, PAULA M.
; MONTONE, ROSALINDA C.
; ALVES, RODRIGO P.
; DE QUEIROZ, ALINE C.
; DE OLIVEIRA, VALÉRIA M.
; DOS SANTOS, VIVIANE P.
; PUTZKE, JAIR
; ROSA, LUIZ HENRIQUE
; DUARTE, ALYSSON W.F.
.
Anais da Academia Brasileira de Ciências
- Métricas do periódico
Abstract In the Antarctic environment, yeasts are versatile eukaryotes that have shown wide dispersion in different substrates, producing active enzymes in extreme conditions, but their relevance in biotechnological applications is largely unknown. The aim of this study was to evaluate the production of extracellular hydrolases by yeasts isolated from Antarctic lichens and molecularly identify these isolates. From a total of 144 isolates on the screening, 109 (76%) produced at least one of the hydrolases tested, with most activities for proteases 59 (41%), cellulases 58 (40%), esterases 57 (39%), lipases 29 (20%), amylases 23 (16%) and pectinases 20 (14%). Among these isolates, 76 were identified, most belonged to the phylum Basidiomycota (n=73) with the dominance of Vishniacozyma victoriae (n=27), Cystobasidium alpinum (n=3), Mrakia niccombsii (n=3), Cystobasidium laryngis (n=2), Bannozyma yamatoana (n=2), Holtermanniella nyarrowii (n=2), and Glaciozyma martinii (n=2). This study is the first one reporting extracellular enzyme production by yeasts isolated from thallus of the species of Antarctic lichens Lecania brialmontii, Polycauliona candelaria, Usnea capillacea, Cladonia metacorallifera, and Polycauliona regalis. With these data, it’s possible to confirm lichens as a source of hydrolase-producing yeasts, reinforcing the potential of these microorganisms in bioprospecting studies of catalytic molecules from polar regions that may be useful in promising biotechnological applications.
9.
Differentials in death count records by databases in Brazil in 2010
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Diógenes, Victor Hugo Dias
; Pinto Júnior, Elzo Pereira
; Gonzaga, Marcos Roberto
; Queiroz, Bernardo Lanza
; Lima, Everton E. C.
; Costa, Lilia Carolina C. da
; Rocha, Aline S.
; Ferreira, Andrêa J. F.
; Teixeira, Camila S. S.
; Alves, Flávia Jôse O.
; Rameh, Leila
; Flores-Ortiz, Renzo
; Leyland, Alastair
; Dundas, Ruth
; Barreto, Maurício L.
; Ichihara, Maria Yury Travassos
.
ABSTRACT OBJECTIVE To compare the death counts from three sources of information on mortality available in Brazil in 2010, the Mortality Information System (SIM - Sistema de Informações sobre Mortalidade ), Civil Registration Statistic System (RC - Sistema de Estatísticas de Resgistro Civil ), and the 2010 Demographic Census at various geographical levels, and to confirm the association between municipal socioeconomic characteristics and the source which showed the highest death count. METHODS This is a descriptive and comparative study of raw data on deaths in the SIM, RC and 2010 Census databases, the latter held in Brazilian states and municipalities between August 2009 and July 2010. The percentage of municipalities was confirmed by the database showing the highest death count. The association between the source of the highest death count and socioeconomic indicators - the Índice de Privação Brasileiro (IBP – Brazilian Deprivation Index) and Índice de Desenvolvimento Humano Municipal (IHDM – Municipal Human Development Index) - was performed by bivariate choropleth and Moran Local Index of Spatial Association (LISA) cluster maps. RESULTS Confirmed that the SIM is the database with the highest number of deaths counted for all Brazilian macroregions, except the North, in which the highest coverage was from the 2010 Census. Based on the indicators proposed, in general, the Census showed a higher coverage of deaths than the SIM and the RC in the most deprived (highest IBP values) and less developed municipalities (lowest IDHM values) in the country. CONCLUSION The results highlight regional inequalities in how the databases chosen for this study cover death records, and the importance of maintaining the issue of mortality on the basic census questionnaire.
10.
Antarctic organisms as a source of antimicrobial compounds: a patent review
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SILVA, MAURÍCIO B.
; FEITOSA, ALEXYA O.
; LIMA, IGOR G.O.
; BISPO, JAMES R.S.
; SANTOS, ANA CAROLINE M.
; MOREIRA, MAGNA S.A.
; CÂMARA, PAULO E.A.S.
; ROSA, LUIZ HENRIQUE
; OLIVEIRA, VALÉRIA M.
; DUARTE, ALYSSON W.F.
; QUEIROZ, ALINE C.
.
Anais da Academia Brasileira de Ciências
- Métricas do periódico
Abstract Currently, antimicrobial resistance has become a global public health problem, which has made the need for new antimicrobial compounds to deal with resistant infections an emergency. However, environments that once offered so many innovative molecules, now already exhaustively exploited, do not meet this need. In this context, a geographically isolated, under-explored and extreme environment, such as Antarctica, which holds organisms with unique physiological and biochemical characteristics, assumes great importance as a potential source of new compounds with antimicrobial activity. In this patent review, we investigate the state of technological development in the field of antimicrobial compounds obtained from Antarctic organisms, highlighting the main countries and researchers active in the field, the species utilized, the compounds obtained, and their possible therapeutic applications. As results, few patent documents were found, however they encompass a wide diversity of compounds and species, indicating a great antimicrobial potential present in Antarctic biota, including compounds active against the most important human pathogenic microorganisms, such as including methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus spp. and multi-resistant Mycobacterium tuberculosis. Furthermore, due to the increasing trend in patent applications, a significant rise in the number of patents in this area is expected in the coming years.
11.
Biological differences and unilateral cross-protection between biotypes of Cowpea aphid-borne mosaic virus
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Maia, Laianny Morais
; Lima, José Albersio de Araujo
; Nascimento, Aline Kelly Queiroz do
; Rabelo Filho, Francisco de Assis Câmara
.
RESUMO Maracujazeiro (Passiflora edulis) e feijoeiro caupi (Vigna unguiculata) são duas culturas economicamente importante para o Nordeste do Brasil. O endurecimento dos frutos causado por isolados de Cowpea aphid-borne mosaic virus biótipo P (CABMV-P) é a virose mais importante que ocorre no maracujazeiro no Brasil e o biótipo caupi (CABMV-C) é responsável pela virose mais comum e importante que afeta o feijoeiro caupi. O objetivo desta pesquisa foi determinar as relações biológicas entre isolados do biótipo CABMV-C obtido de feijoeiro caupi (CABMV-CFor e CABMV-CBv) e isolados do biótipo CABMV-P obtidos de maracujazeiro (CABMV-PFraco e CABMV-PSevera) em hospedeiros comuns. Em estudos de gama de plantas hospedeiros, envolvendo 25 espécies de plantas, somente Canavalia ensiformis e Macroptilium lathyroides mostraram ser hospedeiros sistêmicos comuns a todos os isolados virais. Estudos de proteção cruzada foram desenvolvidos entre CABMV-PFraco e CABMV-PSevero em maracujazeiro e CABMV-PSevero e CABMV-CFor em C. ensiformis. Proteção cruzada unilateral foi detectada entre CABMV-PSevero e CABMV-CFor em C. ensiformes, mas CABMV-PFraco não protegeu maracujazeiro contra o CABMV-PSevero.
ABSTRACT Passion fruit (Passiflora edulis) and cowpea (Vigna unguiculata) are two agricultural crops of considerable economic importance in Northeastern Brazil. Fruit woodiness caused by isolates of Cowpea aphid-borne mosaic virus biotype P (CABMV-P) is the most important virus disease that occurs on passion fruit in Brazil and the biotype cowpea (CABMV-C) is responsible for the most common and important virus disease of cowpea in Northeastern Brazil. The objective of the present research was to determine the biological relationships between isolates of biotype CABMV-C obtained from cowpea (CABMV-CFor and CABMV-CBv) and isolates of biotype CABMV-P obtained from passion fruit (CABMV-PMild and CABMV-PSevere) in common hosts. In host range studies involving 25 plant species only Canavalia ensiformis and Macroptilium lathyroides were shown to be common systemic hosts for all virus isolates. Cross protection studies were developed between CABMV-PMild and CABMV-PSevere in passion fruit and between CABMV-PSevere and CABMV-CFor in C. ensiformis. Unilateral cross protection was detected between CABMV-PSevere and CABMV-CFor in C. ensiformis, but CABMV-PMild did not cross protect passion fruit against CABMV-PSevere.
https://doi.org/10.5935/1806-6690.20170036
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12.
Influence of host preference, mating, and release density on the parasitism of Telenomus remus (Nixon) (Hymenoptera, Platygastridae)
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Queiroz, Ana Paula de
; Bueno, Adeney de Freitas
; Pomari-Fernandes, Aline
; Bortolotto, Orcial Ceolin
; Mikami, Adriana Yatiem
; Olive, Lopes
.
ABSTRACT We evaluated the influence of host preference, mating, and release density on Telenomus remus (Nixon, 1937) (Hymenoptera: Platygastridae) parasitizing eggs of Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae). First, we tested host preference of T. remus (free choice test) offered a choice between eggs of Corcyra cephalonica (Stainton, 1865) (Lepidoptera: Pyralidae) and S. frugiperda. Parasitism capacity and host preference (S. frugiperda) of T. remus reared on either of the two hosts did not differ. Secondly, we evaluated the influence of mating behavior of T. remus females on its parasitism. Only the offspring sex ratio differed between treatments, indicating that the species reproduces by parthenogenesis of the arrhenotoky type. Finally, we evaluated the influence of release density on T. remus parasitism. This was tested by releasing different numbers of the parasitoid per S. frugiperda egg using T. remus reared for different numbers of generations on C. cephalonica eggs. The regression analysis between percentage of parasitism and density of released T. remus females showed a quadratic effect for all tested parasitoid generations (F35, F40, and F45) with maximum parasitism from 65.07% to 71.69%. Our results allow the conclusion that (a) T. remus prefers S. frugiperda eggs, regardless of the host on which this parasitoid was reared, showing no preimaginal conditioning; (b) Mating does not affect the number of eggs parasitized by T. remus or the development of its offspring; and (c) The optimal T. remus release density when reared on C. cephalonica is between 0.133 and 0.150 females/S. frugiperda.
https://doi.org/10.1016/j.rbe.2016.12.004
1056 downloads
13.
Biometria corporal e parâmetros hematológicos de Trachemys scripta elegans e Trachemys dorbignyi (Testudines: Emydidae) criadas em cativeiro em Petrolina, Pernambuco
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Gradela, Adriana
; Souza, Viviane N.
; Queiroz, Manueli M. de
; Constantino, Aline da C.
; Bandeira, Claudio G.C.
; Faria, Marcelo D. de
; Milanelo, Liliane
; Franzo, Vanessa S.
; Corrêa, Fábio Mathias
.
RESUMO: Este estudo objetivou avaliar a biometria corporal e o perfil hematológico de Trachemys scripta elegans (N=28) e de Trachemys dorbignyi (N=22) criadas em cativeiro na região do submédio do Vale do São Francisco, semiárido nordestino brasileiro, visando estabelecer valores sanguíneos básicos de saúde e gerar dados úteis na fisiologia comparativa de Testudines. Após 120 dias de adaptação e jejum de 24 horas, 2,5 mL de sangue foram coletados do seio occipital dorsal e depositados em tubo com heparina sódica para a avaliação, na sequência, dos níveis hematologicos. A contagem total de eritrócitos (CTE) e global de leucócitos (CGL) foi realizada em câmara de Neubauer; a dosagem de hemoglobina (HGB) pelo método da método da cianometahemoglobina e o hematócrito (HCT) através da técnica do microhematócrito. A partir da CTE estabeleceram-se matematicamente os índices hematimétricos. A biometria corporal também foi avaliada: a) massa corporal (MC, g); b) dimensões máximas da carapaça [comprimento (CMC, cm) e largura (LMC, cm)];c) dimensões máximas do plastrão [comprimento (CMP, cm) e largura (LMP, cm)]; d) comprimento total da cauda (CTC, cm); e) comprimento linear da base da cauda ao orifício cloacal (CprC, cm); f) comprimento linear do orifício cloacal a extremidade da cauda(CpoC, cm). T. scripta elegans apresentaram valores maiores (P < 0,05) para a biometria corporal, enquanto que o CTC e CprC foram maiores (P<0,05) em T dorbignyi. Os níveis hematológicos não diferiram (P>0,05) entre as espécies. Os resultados demostram que a maior parte da variação observada entre T. scripta elegans e T. dorbignyi é explicada pelas variáveis biométricas e que algumas correlações hematológicas caracterizam diferenças interespecíficas. Conclui-se que os resultados lançam luz sobre valores de referência para estas espécies mantidas em cativeiro na região do semiárido e servem como um modelo para a fisiologia comparativa intra e interespécies.
ABSTRACT: This study aimed to evaluate the body biometry and hematological profile of Trachemys scripta elegans (N=28) and Trachemys dorbignyi (N=22) reared in captivity in the Brazilian submedium northeastern semi-arid region in the Valley of the São Francisco river. It aimed to establish basic health blood values and generate useful data on the comparative physiology of Testudines. After 120-day adaptation and 24-hour fasting, 2.5mL of blood were collected from the dorsal occipital sinus and deposited into a tube with sodium heparin for evaluation, following, of hematological levels. The red blood cell count (RBC) and GLC was conducted in a Neubauer chamber, the hemoglobin level (HGB) was supplied by the cyanmethemoglobin method and the hematocrit (HCT) was obtained by the microhematocrit technique. Based on the RBC, the hematimetric were mathematically established. Body biometry were also evaluated: a) body mass (BM, g); b) maximum dimensions of the carapace [length (MLC, cm) and width (MWC, cm)]; c) maximum dimensions of plastron [length (MLP, cm) and width (MWP, cm)]; d) total length of tail (TLT, cm); e) linear length from the base of the tail to the cloacal orifice (LPrC, cm); f) linear length from the cloacal orifice to the extremity of the tail (LPoC, cm). T. scripta elegans showed higher values (P<0.05) for biometrics, while TLT and LPrC were higher (P<0.05) in T. dorbignyi. The hematological values did not differ (P>0.05) among species. The results show that most of the variation found between T. scripta elegans and T. dorbignyi is explained by the biometric variables and that some hematologic correlations characterize interspecies differences. It was conclude that the results shed light on benchmarks for these species kept in captivity in the northeastern semi-arid region and serve as a model for intra and interspecies comparative physiology.
https://doi.org/10.1590/s0100-736x2017000100014
3025 downloads
14.
Caracterização biológica e sorológica de um isolado de Squash mosaic virus e efeitos da infecção mista com vírus do gênero Potyvirus (22)
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Silva, Fabiana Rodrigues da
; Lima, José Albersio de Araújo
; Nascimento, Aline Kelly Queiroz do
; Barbosa, Graziela Silva
.
RESUMO O Nordeste brasileiro possui condições edafoclimáticas favoráveis ao cultivo do meloeiro (Cucumis melo). No entanto, diferentes problemas fitossanitários afetam sua produtividade, destacando-se as doenças ocasionadas por vírus. A presente pesquisa objetivou efetuar a caracterização biológica e sorológica de um isolado de Squash mosaic virus (SqMV) obtido no Ceará. Em estudos de gama de hospedeiros o vírus infetou sistemicamente somente cinco espécies da família Cucurbitaceae e ocasionou lesões locais em Chenopodium amaranticolor e C. quinoa. Estudos da infecção mista do SqMV com vírus do gênero Potyvirus demonstraram efeito sinérgico entre os mesmos. A purificação química do SqMV foi possível a partir de plantas de meloeiro e a imunização em coelho com a preparação purificada possibilitou a produção de antissoro policlonal específico. O antissoro produzido apresentou títulos de 1:10.000 quando avaliado por plate trapped antigen enzyme linked immune absorbent assay (PTA-ELISA), de 1:160.000 em immune-precipitation ELISA (IP-PTA-ELISA) e de 1:1.024 em Dupla Difusão em Ágar. Estudos do relacionamento sorológico entre SqMV e Cowpea severe mosaic virus (CPSMV) do gênero Comovirus contra seus antissoros homólogos e heterólogos em Dupla Difusão em Ágar demonstraram relacionamento unilateral entre os mesmos.
ABSTRACT The conditions of soil and climate in the northeast of Brazil are favorable to cultivation of melon (Cucumis melo). However, there are various sanitary problems that affect productivity, especially diseases caused by viruses. This research had the objective of carrying out the biological and serological characterisation of an isolate of the Squash mosaic virus (SqMV), obtained in the State of Ceará, Brazil. In studies into host range, the virus infected systemically only five species of Cucurbitaceae family, and caused local lesions in Chenopodium amaranticolor and C. quinoa. Mixed-infection studies of SqMV with a virus specie from the genus Potyvirus demonstrated a synergistic effect between the viruses. Chemical purification of SqMV was possible using melon plants, and immunisation of rabbits with the purified preparation enabled the production of a specific polyclonal antiserum. The antiserum produced presented titers of 1:10,000 when evaluated by plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA), 1:160,000 by immunoprecipitation ELISA (IP-PTA-ELISA) and 1:1,024 by agar double diffusion. Studies by agar double diffusion of the serological relationship between SqMV and the Cowpea severe mosaic virus (CPSMV), of the genus Comovirus, demonstrated a unilateral relationship against their homologous and heterologous antisera.
https://doi.org/10.5935/1806-6690.20160023
2317 downloads
15.
Chemical constituents from Sidastrum paniculatum and evaluation of their leishmanicidal activity
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Teles, Yanna C.F.
; Chaves, Otemberg S.
; Agra, Maria de Fátima
; Batista, Leônia Maria
; Queiroz, Aline C. de
; Araújo, Morgana V. de
; Alexandre-Moreira, Magna Suzana
; Braz-Filho, Raimundo
; Souza, Maria de Fátima V. de
.
Revista Brasileira de Farmacognosia
- Métricas do periódico
AbstractSidastrum paniculatum (L.) Fryxell, Malvaceae, is popularly known in Brazil as “malva-roxa” or “malvavisco”. The species is found mainly in Northeast region where it is used by locals to treat spider bites and bee stings. Aiming to identify the chemical compounds from S. paniculatum secondary metabolism and to contribute to the chemotaxonomic knowledge of Malvaceae family, a phytochemical study of S. paniculatum was carried out. Besides that, the isolated compounds were evaluated for antileishmanial activity against promastigotes of Leishmania braziliensis. By using chromatographic techniques the study resulted the isolation of eight compounds: 3-oxo-21β-H-hop-22(29)-ene; sebiferic acid; sitosterol 3-O-β-d-glucopyranoside/stigmasterol 3-O-β-d-glucopyranoside; phaeophytin a; 132(S)-hydroxyphaeophytin a; 132(S)-hydroxy-(173)-ethoxyphaeophorbide a and 7,4′-di-O-methylisoescutellarein. The structure of all isolated compounds was elucidated by spectroscopic analysis, including two-dimensional NMR techniques. In addition, the isolated compounds phaeophytin a; 132(S)-hydroxyphaeophytin a; 132(S)-hydroxy-(173)-ethoxyphaeophorbide a and 7,4′-di-O-methylisoescutellarein exhibited antileishmanial activity against promastigotes of L. braziliensis.
https://doi.org/10.1016/j.bjp.2015.02.002
1554 downloads
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