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au:Pulido, Sergio

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Immunogenic potential and neutralizing ability of a heterologous version of the most abundant three-finger toxin from the coral snake Micrurus mipartitus threefinger three finger
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Giraldo, Luz Elena Romero

; Pulido, Sergio

; Berrío, Mario Andrés Flórez, María Fernanda Rey-Suárez, Paola Núñez-Rangel, Vitelbina Córdoba, Mónica Saldarriaga Pereañez, Jaime Andrés

Journal of Venomous Animals and Toxins including Tropical Diseases

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2024, Volume 30 elocation e20230074

Resumo: En | Texto: En | PDF: En

Abstract Background: Micrurus mipartitus is a coral snake of public health concern in Colombia. Its venom is mainly composed of three-finger toxins (3FTxs), Mipartoxin-1 being the most abundant protein partially responsible for its lethal effect. In this work, we present the production of Mipartoxin-1 in a recombinant form and evaluate its immunogenic potential. Methods: A genetic construct HisrMipartoxin-1 was cloned into the pET28a vector and heterologous expression was obtained in E. coli BL21 (DE3). The recombinant HisrMipartoxin-1 protein was extracted from inclusion bodies, refolded in vitro, and isolated by affinity and RP-HPLC chromatography. The lethal effect of HisrMipartoxin-1 was tested, and antibodies against HisrMipartoxin-1 were produced by immunization in rabbits. The antibody titers were monitored by an ELISA test. The neutralizing ability of the antibodies, against the lethal effect of native toxins and M. mipartitus venom, was also assessed. Results: HisrMipartoxin-1 was detected on SDS-PAGE, with a molecular mass of around 11 kDa. The retention time was 16.0 minutes. HisrMipartoxin-1 did not exhibit lethality in mice; however, antibodies against HisrMipartoxin-1 recognized the native toxin, the whole venom of M. mipartitus, and a 3FTx from another species within the Micrurus genus. Furthermore, antibodies against HisrMipartoxin-1 completely neutralized the lethal effect of native Mipartoxin-1 in mice but not M. mipartitus whole venom. Conclusion: These findings indicate that HisrMipartoxin-1 might be used as an immunogen to develop anticoral antivenoms or complement them. This work is the first report of the heterologous expression of 3FTx from M. mipartitus. Background Colombia threefinger three finger 3FTxs, 3FTxs FTxs , (3FTxs) Mipartoxin1 Mipartoxin 1 Mipartoxin- potential Methods HisrMipartoxin1 HisrMipartoxin HisrMipartoxin- pETa pET E BL BL2 DE3. DE3 DE . (DE3) bodies vitro RPHPLC RP HPLC chromatography tested rabbits test M assessed Results SDSPAGE, SDSPAGE SDS PAGE, PAGE SDS-PAGE kDa 160 16 0 16. minutes however toxin FTx genus Furthermore Conclusion them (3FTxs (DE3 (DE

https://doi.org/10.1590/1678-9199-jvatitd-2023-0074

Peptides DLL37-1 and LL37-1, an alternative to inhibit biofilm formation in clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis
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ALBA, MARYI LORENA SEGURA

; DURÁN-RODRIGUEZ, ANDREA TATIANA

; PULIDO, LUZ MARY SALAZAR

; ESCOBAR-PÉREZ, JAVIER

; GUTIÉRREZ, SERGIO ALEJANDRO

; OSPINA, JEANNETTE NAVARRETE

; BERMÚDEZ, GLADYS PINILLA

; MOLINA, LILIANA CONSTANZA MUÑOZ

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 3 elocation e20210848

Resumo: En | Texto: En | PDF: En

Abstract Staphylococcus aureus and Staphylococcus epidermidis have microbial surface components recognizing adhesive matrix molecules (MSCRAMM) adhesion proteins that enhance their biofilm formation ability, as well as virulence factors that influence morbidity and mortality in hospital settings. In this work, four peptides analogous of the peptide LL-37 that were evaluated to inhibit biofilm formation and its action potential on the expression of MSCRAMM proteins in clinical isolates through different tests, such as crystal violet, PCR and qPCR. In total, 96.8% of S. aureus were strong in biofilm formation in contrast to 48.4% of S. epidermidis. sdrG and sdrF genes were present in 100% of S. epidermidis strains and in all isolates. In S. aureus, specific genes that code for MSCRAMM proteins were detected: clfA (89%), clFB, sdrC and fnBA (94%). The peptides did not show hemolytic or cytotoxic activity. In this study, it was evidenced that of the peptides DLL37-1 at a 5 µM concentration was an efficacious antimicrobial agent and depicted greater biofilm inhibition in both bacterial species. Exhibiting a significant inhibition rate in S. aureus, this peptide caused a negative regulation in the expression of the genes clfA and sdrC, showed greater biological activity.

https://doi.org/10.1590/0001-3765202220210848

Consenso Colombiano para la prevención, diagnóstico y manejo de condiciones trombóticas en pacientes adultos con COVID-19: aplicando el Marco de la Evidencia a la Decisión (EtD) GRADE
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Acevedo-Peña, Juan Yomayusa-González, Nancy Cantor-Cruz, Francy Pinzon-Florez, Carlos Barrero-Garzón, Liliana De-La-Hoz-Siegler, Ilich Low-Padilla, Eduardo Ramírez-Ceron, Carlos Combariza-Vallejo, Felipe Arias-Barrera, Carlos Moreno-Cortés, Javier Rozo-Vanstrahlen, José Correa-Pérez, Liliana Rojas-Gambasica, José González-González, Camilo La-Rotta-Caballero, Eduardo Ruíz-Talero, Paula Contreras-Páez, Rubén Lineros-Montañez, Alberto Ordoñez-Cardales, Jorge Escobar-Olaya, Mario Izaguirre-Ávila, Raúl Campos-Guerra, Joao Accini-Mendoza, José Pizarro-Gómez, Camilo Patiño-Pérez, Adulkarín Flores-Rodríguez, Janine Valencia-Moreno, Albert Londoño-Villegas, Alejandro Saavedra-Rodríguez, Alfredo Madera-Rojas, Ana Caballero-Arteaga, Andrés Díaz-Campos, Andrés Correa-Rivera, Felipe Mantilla-Reinaud, Andrés Becerra-Torres, Ángela Peña-Castellanos, Ángela Reina-Soler, Aura Escobar-Suarez, Bibiana Patiño-Escobar, Bonell Rodríguez-Cortés, Camilo Rebolledo-Maldonado, Carlos Ocampo-Botero, Carlos Rivera-Ordoñez, Carlos Saavedra-Trujillo, Carlos Figueroa-Restrepo, Catalina Agudelo-López, Claudia Jaramillo-Villegas, Claudia Villaquirán-Torres, Claudio Rodríguez-Ariza, Daniel Rincón-Valenzuela, David Lemus-Rojas, Melissa Pinto-Pinzón, Diego Garzón-Díaz, Diego Cubillos-Apolinar, Diego Beltrán-Linares, Edgar Kondo-Rodríguez, Emilio Yama-Mosquera, Erica Polania-Fierro, Ernesto Real-Urbina, Evalo Rosas-Romero, Andrés Mendoza-Beltrán, Fernán Guevara-Pulido, Fredy Celia-Márquez, Gina Ramos-Ramos, Gloria Prada-Martínez, Gonzalo León-Basantes, Guillermo Liévano-Sánchez, Guillermo Ortíz-Ruíz, Guillermo Barreto-García, Gustavo Ibagón-Nieto, Harold Idrobo-Quintero, Henry Martínez-Ramírez, Ingrid Solarte-Rodríguez, Ivan Quintero-Barrios, Jorge Arenas-Gamboa, Jaime Pérez-Cely, Jairo Castellanos-Parada, Jeffrey Garzón-Martínez, Fredy Luna-Ríos, Joaquín Lara-Terán, Joffre Vargas-Fodríguez, Johanna Dueñas-Villamil, Rubén Bohórquez-Feyes, Vicente Martínez-Acosta, Carlos Gómez-Mesa, Esteban Gaitán-Rozo, Julián Cortes-Colorado, Julián Coral-Casas, Juliana Horlandy-Gómez, Laura Bautista-Toloza, Leonardo Palacios Palacios, Leonardo Fajardo-Latorre, Lina Pino-Villarreal, Luis Rojas-Puentes, Leonardo Rodríguez-Sánchez, Patricia Herrera-Méndez, Mauricio Orozco-Levi, Mauricio Sosa-Briceño, Mónica Moreno-Ruíz, Nelson Sáenz-Morales, Oscar Amaya-González, Pablo Ramírez-García, Sergio Nieto-Estrada, Víctor Carballo-Zárate, Virgil Abello-Polo, Virginia

Acta Medica Colombiana

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Mar 2021, Volume 46 Nº 1 Páginas 51 - 72

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Recent studies have reported the occurrence of thrombotic phenomena or coagulopathy in patients with COVID-19. There are divergent positions regarding the prevention, diagnosis, and treatment of these phenomena, and current clinical practice is based solely on deductions by extension from retrospective studies, case series, observational studies, and international guidelines developed prior to the pandemic. In this context, the aim was to generate a group of recommendations on the prevention, diagnosis and management of thrombotic complications associated with COVID-19. Methods: A rapid guidance was carried out applying the GRADE Evidence to Decision (EtD) frameworks and an iterative participation system, with statistical and qualitative analysis. Results: 31 clinical recommendations were generated focused on: a) Coagulation tests in symptomatic adults with suspected infection or confirmed SARS CoV-2 infection; b) Thromboprophylaxis in adults diagnosed with COVID-19 (Risk scales, thromboprophylaxis for outpatient, in-hospital management, and duration of thromboprophylaxis after discharge from hospitalization), c) Diagnosis and treatment of thrombotic complications, and d) Management of people with previous indication of anticoagulant agents. Conclusions: Recommendations of this consensus guide clinical decision-making regarding the prevention, diagnosis, and treatment of thrombotic phenomena in patients with COVID-19, and represent an agreement that will help decrease the dispersion in clinical practices according to the challenge imposed by the pandemic.

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https://doi.org/10.36104/amc.2021.2078

Consenso colombiano para la prevención, el diagnóstico y el tratamiento de condiciones trombóticas en adultos con COVID-19: aplicando el Marco de la Evidencia a la Decisión (EtD) GRADE
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Acevedo-Peña, Juan Yomayusa-González, Nancy Cantor-Cruz, Francy Pinzón-Flórez, Carlos Barrero-Garzón, Liliana De-La-Hoz-Siegler, Ilich Low-Padilla, Eduardo Ramírez-Cerón, Carlos Combariza-Vallejo, Felipe Arias-Barrera, Carlos Moreno-Cortés, Javier Rozo-Vanstrahlen, José Correa-Pérez, Liliana Rojas-Gambasica, José González-González, Camilo La-Rotta-Caballero, Eduardo Ruíz-Talero, Paula Contreras-Páez, Rubén Lineros-Montañez, Alberto Ordoñez-Cardales, Jorge Escobar-Olaya, Mario Izaguirre-Ávila, Raúl Campos-Guerra, Joao Accini-Mendoza, José Pizarro-Gómez, Camilo Patiño-Pérez, Adulkarín Flores-Rodríguez, Janine Valencia-Moreno, Albert Londoño-Villegas, Alejandro Saavedra-Rodríguez, Alfredo Madera-Rojas, Ana Caballero-Arteagam, Andrés Díaz-Campos, Andrés Correa-Rivera, Felipe Mantilla-Reinaud, Andrés Becerra-Torres, Ángela Peña-Castellanos, Ángela Reina-Soler, Aura Escobar-Suarez, Bibiana Patiño-Escobar, Bonell Rodríguez-Cortés, Camilo Rebolledo-Maldonado, Carlos Ocampo-Botero, Carlos Rivera-Ordoñez, Carlos Saavedra-Trujillo, Carlos Figueroa-Restrepo, Catalina Agudelo-López, Claudia Jaramillo-Villegas, Claudia Villaquirán-Torres, Claudio Rodríguez-Ariza, Daniel Rincón-Valenzuela, David Lemus-Rojas, Melissa Pinto-Pinzón, Diego Garzón-Díaz, Diego Cubillos-Apolinar, Diego Beltrán-Linares, Edgar Kondo-Rodríguez, Emilio Yama-Mosquera, Erica Polania-Fierro, Ernesto Real-Urbina, Evalo Rosas-Romero, Andrés Mendoza-Beltrán, Fernán Guevara-Pulido, Fredy Celia-Márquez, Gina Ramos-Ramos, Gloria Prada-Martínez, Gonzalo León-Basantes, Guillermo Liévano-Sánchez, Guillermo Ortíz-Ruíz, Guillermo Barreto-García, Gustavo Ibagón-Nieto, Harold Idrobo-Quintero, Henry Martínez-Ramírez, Ingrid Solarte-Rodríguez, Ivan Quintero-Barrios, Jorge Arenas-Gamboa, Jaime Pérez-Cely, Jairo Castellanos-Parada, Jeffrey Garzón-Martínez, Fredy Luna-Ríos, Joaquín Lara-Terán, Joffre Vargas-Rodríguez, Johanna Dueñas-Villamil, Rubén Bohórquez-Reyes, Vicente Martínez-Acosta, Carlos Gómez-Mesa, Esteban Gaitán-Rozo, Julián Cortes-Colorado, Julián Coral-Casas, Juliana Horlandy-Gómez, Laura Bautista-Toloza, Leonardo Palacios, Leonardo Palacios Fajardo-Latorre, Lina Pino-Villarreal, Luis Rojas-Puentes, Leonardo Rodríguez-Sánchez, Patricia Herrera-Méndez, Mauricio Orozco-Levi, Mauricio Sosa-Briceño, Mónica Moreno-Ruíz, Nelson Sáenz-Morales, Oscar Amaya-González, Pablo Ramírez-García, Sergio Nieto-Estrada, Víctor Carballo-Zárate, Virgil Abello-Polo, Virginia

Revista Colombiana de Cardiología

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Out 2020, Volume 27 Nº 5 Páginas 446 - 460

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Introduction: recent studies have reported the occurrence of thrombotic phenomena or coagulopathy in patients with COVID-19. There are divergent positions regarding the prevention, diagnosis, and treatment of these phenomena, and current clinical practice is based solely on deductions by extension from retrospective studies, case series, observational studies, and international guidelines developed prior to the pandemic. Objective: to generate a group of recommendations on the prevention, diagnosis and management of thrombotic complications associated with COVID-19. Methods: a rapid guidance was carried out applying the GRADE Evidence to Decision (EtD) frameworks and an iterative participation system, with statistical and qualitative analysis. Results: 31 clinical recommendations were generated focused on: a) Coagulation tests in symptomatic adults with suspected infection or confirmed SARS CoV-2 infection; b) Thromboprophylaxis in adults diagnosed with COVID-19 (Risk scales, thromboprophylaxis for outpatient, in-hospital management, and duration of thromboprophylaxis after discharge from hospitalization), c) Diagnosis and treatment of thrombotic complications, and d) Management of people with previous indication of anticoagulant agents. Conclusions: recommendations of this consensus guide clinical decision-making regarding the prevention, diagnosis, and treatment of thrombotic phenomena in patients with COVID-19, and represent an agreement that will help decrease the dispersion in clinical practices according to the challenge imposed by the pandemic.

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https://doi.org/10.1016/j.rccar.2020.08.003

Identification and quantification of limonoid aglycones content of Citrus seeds
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Montoya, Carlos

; González, Luis

; Pulido, Sergio

; Atehortúa, Lucía

; Robledo, Sara M.

Revista Brasileira de Farmacognosia

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Dez 2019, Volume 29 Nº 6 Páginas 710 - 714

Resumo: En | Texto: En | PDF: En

ABSTRACT Citrus fruits are recognized as an important source of bioactive molecules such as limonin and nomilin. However, these molecules exhibit low bioavailability, therefore, obtaining these molecules using biotechnological techniques may be an alternative to harvesting them directly from fruits. The aim of this study was to quantify and identify limonoids in the dichloromethane extracts of Citrus seeds of Criolla orange, Oneco tangerine, Tangerine-lemon, Sour orange and Valencia orange from department of Antioquia-Colombia by high performance liquid chromatography with diode array detection, and high-resolution mass spectrometry. Although in all the samples total glycosidic free limonoids were present, Oneco tangerine seeds had the highest concentration, followed by Tangerine-lemon seeds, equivalent to 0.75% and 0.53% per total dry weight, respectively. These results suggest Oneco tangerine seeds may be used as an elite material for biotechnological processes looking for increased production of limonoids to support research and drug development.

https://doi.org/10.1016/j.bjp.2019.07.006 594 downloads

Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp.
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Verdugo-González, Lucía Acosta-Cárdenas, Alejandro Zapata-Zapata, Arley David Cadena-Chamorro, Edith M. Pulido-Muñoz, Sergio Andrés

DYNA

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Dez 2019, Volume 86 Nº 211 Páginas 192 - 198

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Microalgae are photosynthetic microorganisms widely used at the industrial level because of their high content of compounds with bioactive properties such as antimicrobial peptides. These compounds have a wide range of action mechanisms, do not produce the resistance of conventional antibiotics and could be effective against bacteria, fungi, viruses, parasites, and even tumor cells. In this work, by mechanical cell lysis treatment, a protein extract of dry biomass of Nannochloropsis sp was obtained, subsequently, it was hydrolyzed with an enzyme complex of proteases, papain, and pancreatin, for 6 hours, at 44 ° C and pH 7. Finally, the antimicrobial activity of the fraction of hydrolyzed extract with a size less than 3 kDa was evaluated against Staphylococcus aureus, Escherichia coli, and Candida albicans, obtaining a percentage of growth inhibition of 93.1%, 96.6%, and 14.4% respectively, which represents a peptic extract with bacteriostatic and fungistatic properties.

Resumen Las microalgas son microorganismos fotosintéticos ampliamente utilizados a nivel industrial gracias a su alto contenido de compuestos con propiedades bioactivas como lo son los péptidos antimicrobianos. Estos compuestos son eficaces contra bacterias, hongos, virus, parásitos e incluso células tumorales, poseen una amplia variedad de mecanismos de acción y no generan la resistencia de los antibióticos convencionales. En este trabajo, a través de lisis celular mecánica, se obtuvo un extracto de proteínas a partir de biomasa seca de Nannochloropsis sp., posteriormente fue hidrolizado usando un complejo enzimático de proteasas, papaína y pancreatina, durante 6 horas, a 44°C y pH 7. Finalmente, se evaluó la actividad antimicrobiana de la fracción de extracto hidrolizado que poseía un tamaño menor a 3 kDa contra Staphylococcus aureus, Escherichia coli y Candida albicans, obteniendo un porcentaje de inhibición de crecimiento de los microorganismos de 93,1%, 96,6%, y 14,4% respectivamente, lo cual representa un extracto peptídico con propiedades bacteriostáticas y fungistáticas.

https://doi.org/10.15446/dyna.v86n211.78865

Un modelo dinámico para reducir la contaminación en México
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Castro-Ramírez, Juan C. Gutiérrez-Andrade, Miguel A. Omaña-Pulido, Elsa P. De-los-Cobos-Silva, Sergio G.

Revista de Matemática Teoría y Aplicaciones

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Dez 2019, Volume 26 Nº 2 Páginas 339 - 369

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen El objetivo de este trabajo es evaluar la eficacia de una política ambiental que promueva la sustitución de energías fósiles por renovables, con la finalidad de reducir gradualmente las emisiones de dióxido de carbono a lo largo de un periodo. Se utiliza un modelo dinámico de equilibrio general computable para la economía mexicana y se simulan escenarios con dos elasticidades de sustitución, cuando se gravan de manera conjunta a dos de los bienes energéticos que usan combustibles fósiles, midiendo sus impactos sobre la producción agregada y el bienestar social. El análisis de los resultados muestra que la eficacia de la política depende de la elasticidad de sustitución. En el escenario rígido es parcialmente efectiva, ya que se reducen las demandas de todas las energías, fósiles y renovables. El escenario flexible resulta ser eficaz, al favorecer la sustitución entre las energías. La reducción es mayor en el escenario rígido que en el flexible. En cada escenario al incrementar el impuesto disminuyen el PIB y el bienestar social respecto al equilibrio de referencia.

Abstract The objective of this work is to evaluate the effectiveness of an environmental policy that promotes the substitution of fossil fuels for renewable alternatives, to reduce carbon dioxide emissions. A dynamic computable general equilibrium model is used for the Mexican economy simulating economic scenarios with two elasticities of substitution, when two of the energy goods that use fossil fuels are taxed together. The impacts on aggregate production and social well-being are quantified or measured. The analysis shows that the effectiveness of the policy depends on the elasticity of substitution. The rigid scenario is partially effective, since the demands of all the energies, fossils and renewable are reduced. The flexible scenario turns out to be effective, favoring the substitution between energies. The demand of all energies is further reduced in the rigid scenario than in the flexible. In each scenario, increasing the tax reduces GDP and social welfare in relation to the reference equilibrium.

https://doi.org/10.15517/rmta.v26i2.38323

Genotyping of Malassezia pachydermatis disclosed genetic variation in isolates from dogs in Colombia
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Celis-Ramírez, Adriana Marcela

; Guevara-Suarez, Marcela

; Galvis-Marín, Juan Camilo Rodríguez-Bocanegra, María Ximena Castañeda-Salazar, Rubiela Linares-Linares, Melva Yomary Triana-Sierra, Sergio Heli Pulido-Villamarín, Adriana del Pilar

Pesquisa Veterinária Brasileira

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Nov 2019, Volume 39 Nº 11 Páginas 915 - 922

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: Malassezia pachydermatis é uma levedura lipofílica e dependente de lipídios, principalmente da pele de animais. Sendo, por essa razão, considerada uma espécie zoofílica e causadora de otite externa em cães. Neste sentido, aspectos associados à sua epidemiologia e patogenicidade constituem um tema de interesse científico. O objetivo deste estudo foi realizar a caracterização molecular de 43 isolados de M. pachydermatis obtidos a partir de cães com otite externa. Para esta propósito, foram amplificadas, sequenciadas e analisadas com enzimas de restrição as regiões do gene 5.8S, do espaçador interno transcrito 2 (ITS2) e D1/D2 do 26S do rRNA pelo método RFLP, com as endonucleases AluI, CfOI e BstF5I. Análises filogenéticas revelaram que os isolados se agruparam com as sequências tipo I, IV e V de M. pachydermatis como já descrito anteriormente. De maneira interessante, se observou um novo RFLP polimórfico utilizando BstF5I. Os isolados que mostraram esse padrão foram associados com os padrões IV e V. No entanto, não foi observada associação entre padrões polimórficos de RFLP e atividade de fosfolipase ou dados da população canina. Estes resultados demonstram a diversidade genética de M. pachydermatis e fornecem novas perspectivas sobre a epidemiologia destas espécies na população analisada.

ABSTRACT: Malassezia pachydermatis is a lipophilic and lipid-dependent yeast mostly isolated from animals’ skin; hence, it is regarded as a zoophilic species causing otitis externa in dogs. Aspects associated with its epidemiology and pathogenicity is a matter of interest. This study aimed to conduct a molecular characterization of 43 isolates of M. pachydermatis obtained from dogs with otitis externa. For this purpose, the 5.8S internal transcribed spacer 2 (ITS2) and D1/D2 26S rRNA regions were amplified, sequenced and analyzed using restriction fragment length polymorphism (RFLP) with AluI, CfoI, and BstF5I endonucleases. Phylogenetic analyses revealed that these isolates grouped with the sequence types I, IV and V, previously proposed for M. pachydermatis. Interestingly, we found a new polymorphic RFLP pattern using BstF5I, these isolates were associated with the sequence types IV and V, nevertheless an association between polymorphic RFLP patterns, and fosfolipase activity or canine population data was not observed. These findings underline the genetic diversity of M. pachydermatis and provide new insights about the epidemiology of this species in the analyzed population.

https://doi.org/10.1590/1678-5150-pvb-6158 921 downloads

Treatment adherence in patients more than 65 years who experience early readmissions
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Vicente-Sánchez, Sergio Olmos-Jiménez, Raquel Ramírez-Roig, Cristina García-Sánchez, María José Valderrey-Pulido, Manuel de la Rubia-Nieto, Amelia

Farmacia Hospitalaria

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Ago 2018, Volume 42 Nº 4 Páginas 147 - 151

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Objective: To analyse the rate of therapeutic nonadherence in patients who experience early readmissions, and identify the factors associated with nonadherence. Methods: An observational descriptive 3-month study (March-May 2014), which included all patients more than 65 years who were readmitted between 3 to 30 days following the last hospital discharge. Exclusion criteria: programmed re-admissions and readmissions to the Intensive Care Unit. Variables included in the study: age, sex, medical service, major diagnostic category, polypharmacy, number of days since the last hospital discharge, and hypertension and diabetes. Therapeutic adherence and difficulty in taking medication were assessed using the Morisky-Green test and the Haynes-Sackett test, respectively. A descriptive analysis of the variables was conducted, showing they were associated with therapeutic adherence. Statistically significant variables were included in a multivariate logistic regression model. Results: In total, 57% of the patients were nonadherent to pharmacological treatment; 23% had difficulty taking their medication; 86% had comorbidities (hypertension and diabetes); 79% had a caregiver; and 86% were polymedicated (≥ 5 medications). There was an association between lack of adherence and difficulty in taking medications (P = 0.021), polypharmacy (P = 0.002), and diabetes mellitus (P = 0.018). Conclusions: Polymedication, diabetes mellitus, and difficulty in taking medication were shown to be prognostic factors of lack of adherence to treatment in patients more than 65 years.

Resumen Objetivo: Analizar la frecuencia del incumplimiento terapéutico en pacientes que sufren reingresos hospitalarios precoces e identificar los factores asociados al mismo. Método: Estudio observacional descriptivo de tres meses de duración (marzo-mayo de 2014). Se incluyeron todos los pacientes mayores de 65 años que reingresaron en los 3-30 días siguientes al alta hospitalaria. Fueron excluidos los reingresos programados y los reingresos en la Unidad de Cuidados Intensivos. Las variables recogidas fueron: edad, sexo, servicio médico, categoría diagnóstica mayor, polimedicación, número de días desde el alta, presencia de hipertensión y/o diabetes. Se evaluó el cumplimiento terapéutico y la dificultad en la administración de medicación mediante el test de Morisky-Green y el test de Haynes-Sackett, respectivamente. Se realizó un análisis descriptivo de las variables y se relacionaron estas con la adherencia terapéutica. Las variables con significación estadística se incluyeron en un modelo de regresión logística multivariante. Resultados: El 57% de los pacientes presentaron falta de adherencia al tratamiento farmacológico. El 23% presentaba dificultad en la administración de la medicación. Un 86% presentaba comorbilidades (hipertensión y/o diabetes) y el 79% tenía cuidador. El 86% de los pacientes estaban polimedicados (≥ 5 fármacos). Existe relación entre la falta de adherencia y la dificultad en la administración de los medicamentos (p = 0,021), la polimedicación (p = 0,002) y la presencia de diabetes mellitus (p=0,018). Conclusiones: La polimedicación, la presencia de diabetes mellitus y la existencia de dificultad en la administración de la medicación se evidencian como factores pronósticos de la falta de adherencia al tratamiento en pacientes mayores de 65 años.

https://doi.org/10.7399/fh.10907

10.

Concentraciones de 25-hidroxivitamina D en embarazadas con y sin diabetes gestacional
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Camarena-Pulido, Eva Elizabet Corona-Gutiérrez, América Aimé Avilés-Almanza, Cristóbal González-Moreno, Jorge Fajardo-Dueñas, Sergio López-Aceves, Luis Javier

Ginecología y obstetricia de México

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2018, Volume 86 Nº 10 Páginas 658 - 664

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Objective: To compare the levels of vitamin (25[OH]D) between patients with or without gestational diabetes. Materials and methods: Analytical cross-sectional study that consisted in taking blood samples to determine and compare the concentrations of (25[OH]D) vitamin D. The sample size was previously determined considering a prevalence of 35% for vitamin D deficiency, an alpha error of 0.05 and a beta error of 0.80 for which 784 pregnant women were recruited, 394 patients with gestational diabetes and 390 without gestational diabetes. Results: In the group of patients without gestational diabetes, 51% (n = 199) had sufficient levels, 33% (n = 129) insufficient levels and 16% (n = 62) deficient levels. In the group of patients with gestational diabetes, 12% (n = 47) had sufficient levels, 67% (n = 264) insufficient levels and 21% (n = 83) deficient levels. The results obtained show that 88.9% (n = 350) of women with gestational diabetes present with levels lower than 30 ng/mL vs 48.5% (n = 189) of the group without gestational diabetes [p <0.00001 OR 7.69 (IC95%: 5.3 - 11)]. Conclusions: Levels below 30 ng/mL of total vitamin D are more frequent in patients with gestational diabetes.

Resumen Objetivo: Comparar las concentraciones de 25-hidroxivitamina D (25[OH]D) en pacientes con y sin diabetes gestacional. Materiales y métodos: Estudio observacional, transversal y analítico consistente en la toma de muestras sanguíneas para determinar y comparar las concentraciones de 25-hidroxivitamina D. El tamaño de la muestra se determinó previamente con base en una prevalencia de 35% para la deficiencia de vitamina D, un error alfa de 0.05 y un error beta de 0.80. Resultados: Se reclutaron 784 embarazadas, 394 con diabetes gestacional y 390 sin este padecimiento. En este último grupo 51% (n = 199) tuvieron concentraciones suficientes, 33% (n = 129) concentraciones insuficientes y 16% (n = 62) concentraciones deficientes. En el grupo de mujeres con diabetes gestacional, 12% (n = 47) tuvieron concentraciones suficientes, 67% (n = 264) concentraciones insuficientes y 21% (n = 83) concentraciones deficientes. Los resultados obtenidos muestran que 88.9% (n = 350) de las mujeres con diabetes gestacional tenían concentraciones inferiores a 30 ng/mL vs 48.5% (n = 189) del grupo sin diabetes gestacional (p < 0.00001; RM: 7.69; IC95%: 5.3-11). Conclusiones: Las concentraciones inferiores a 30 ng/mL de vitamina D total son más frecuentes en pacientes con diabetes gestacional.

https://doi.org/10.24245/gom.v86i10.2262

11.

Indicaciones del factor VII recombinante activado en hemorragia obstétrica grave
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Corona-Gutiérrez, América Aime García-Ruan, Karen Camarena-Pulido, Eva Elizabet López-Aceves, Luis Javier González-Moreno, Jorge Fajardo-Dueñas, Sergio

Ginecología y obstetricia de México

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2018, Volume 86 Nº 12 Páginas 779 - 786

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract OBJECTIVE: To describe outcome of patients with severe obstetric hemorrhage (≥ 1000 mL) treated with rFVIIa as part of the management and to detect complications related to its use. MATERIALS AND METHODS: Retrospective, cross-sectional and descriptive study carried out in patients with severe obstetric hemorrhage treated at the Hospital Civil de Guadalajara Dr. Juan I Menchaca between 2001 and 2017 and treated with activated recombinant factor VII. We identified relevant antecedents, average dose and number of doses of rFVIIa, and hemostatic response. We determined if quantity of bleeding, administration of blood products and hematological parameters before and after using rFVIIa was significantly different. RESULTS: We identified ten patients with rFVIIa administration. The cause of severe obstetric hemorrhage was uterine atony in six cases. The average dose of rFVIIa was 91 mcg/kg; one dose was administered in eight patients and two doses in two patients. Hemostasis was achieved in all patients, bleeding decreased significantly after administration of rFVIIa (5075 mL vs 928 mL, p = 0.000) and the number of erythrocyte concentrates required 7vs 3, p = 0.006). One patient did not require a hysterectomy after rFVIIa administration; one patient presented pulmonary thromboembolism and recovered without complications, no patient died. CONCLUSION: rFVIIa administration as a hemostatic in severe obstetric hemorrhage had favorable results, preventing hysterectomy in one patient. Follow-up requires close monitoring of thrombosis.

Resumen OBJETIVO: Describir la situación final de salud de las pacientes con hemorragia obstétrica grave (≥ 1000 mL) en quienes se indicó factor VII recombinante activado como parte del tratamiento e identificar las complicaciones atribuibles a este medicamento. MATERIALES Y MÉTODOS: Estudio retrospectivo, transversal y descriptivo efectuado en pacientes con hemorragia obstétrica grave atendidas en el Hospital Civil de Guadalajara Dr. Juan I Menchaca entre 2001 y 2017 y tratadas con factor VII recombinante activado. Se identificaron los antecedentes de importancia y se calculó la dosis promedio y cantidad de dosis de factor VII recombinante activado; se valoró la respuesta hemostática y se determinó si la diferencia en cantidad de sangrado, administración de hemoderivados y parámetros hematológicos antes y después de utilizar factor VII recombinante activado fue significativa. RESULTADOS: Se identificaron 10 pacientes en quienes se aplicó factor VII recombinante activado. La causa de hemorragia obstétrica grave fue atonía uterina en seis casos. La dosis promedio de factor VII recombinante activado fue de 91 mcg/kg. En 8 pacientes se administró una dosis y 2 dosis en 2 pacientes. En todas las pacientes se logró la hemostasia; el sangrado disminuyó significativamente posterior a la administración del factor VII recombinante activado (5075 vs 928 mL; p = 0.000) lo mismo que la cantidad de concentrados eritrocitarios trasfundidos (7 vs 3; p = 0.006). Una paciente no requirió histerectomía, otra tuvo tromboembolia pulmonar, que se trató sin problemas y ninguna paciente falleció. CONCLUSIÓN: El factor VII recombinante activado como hemostático en hemorragia obstétrica grave mostró resultados favorables y evitó la histerectomía en una paciente. Requiere vigilancia estrecha de las complicaciones trombóticas.

https://doi.org/10.24245/gom.v86i12.2312

12.

EVAPORACIÓN OSMÓTICA: FUNDAMENTOS Y APLICACIONES EN LA CONCENTRACIÓN DE JUGOS DE FRUTA
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PULIDO-DÍAZ, ADRIANA PATRICIA FORERO-LONGAS, FREDDY CABRERA-NAVARRO, SERGIO ANDRÉS

Biotecnología en el Sector Agropecuario y Agroindustrial

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Dez 2016, Volume 14 Nº 2 Páginas 135 - 144

Resumo: Pt Es En | Texto: Pt Es En | PDF: Pt | PDF: Es | PDF: En

Evaporação osmótica é uma modificação de processos tradicionais que utilizam membranas; onde o efeito de uma diferença de pressão de vapor, produzido por uma solução altamente concentrada de extracção, a água é transferida a forma de vapor através de uma membrana que actua como um corpo hidrofóbico. Esta tecnologia de membranas é apresentado como uma boa alternativa para o processamento de frutos exóticos, produzindo os concentrados que podem ser utilizados na dieta diária, ser mais confortável de usar, reduzindo os custos de transporte e aumentando a vida de serviço. Este processo tem muitas vantagens quando comparado com as operaçõ:es tradicionais, permitindo operado à pressão atmosférica e temperatura baixa, ideal para produtos sensíveis ao calor.

La evaporación osmótica es una modificación de los procesos tradicionales que utilizan membranas; donde por efecto de un diferencial de presión de vapor, producido por una solución de extracción fuertemente concentrada, se transfiere agua solo en forma de vapor a través de una membrana que actúa como cuerpo hidrófobo. Esta tecnología de membranas se presenta como una gran alternativa para el procesamiento de frutas exóticas, produciendo concentrados que pueden ser usados en la dieta diaria, siendo más fáciles de consumir, reduciendo costos de transporte y aumentando la vida útil. Este proceso tiene múltiples ventajas comparado con las operaciones tradicionales de separación, pues permite trabajar a presión atmosférica y baja temperatura, siendo ideal para productos sensibles al calor.

Osmotic evaporation is a modification of traditional processes using membranes; where the effect of a vapor pressure differential, produced by a highly concentrated solution of extraction, water is transferred as vapor through a membrane that acts as a hydrophobic body. This membrane technology is presented as a great alternative for processing exotic fruits, producing concentrates that can be used in the daily diet, being more comfortable to use, reducing transportation costs and increasing the shelf life. This process has many advantages compared to traditional operations, allowing operated at atmospheric pressure and low temperature, ideal for heat-sensitive products.

https://doi.org/10.18684/BSAA(14)135-144

13.

Parasites of the green jack Caranx caballus (Pisces: Carangidae) in three locations from Pacific coasts of Mexico, and their utility as biological tags
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Violante-González, Juan Gallegos-Navarro, Yesenia Monks, Scott García-Ibáñez, Sergio Rojas-Herrera, Agustín A. Pulido-Flores, Griselda Villerías-Salinas, Salvador Larumbe-Morán, Edvino

Revista mexicana de biodiversidad

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Set 2016, Volume 87 Nº 3 Páginas 1015 - 1022

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract: Parasites are known to be useful as biological tags in distinguishing fish stocks, but the parasite fauna method has received limited use in the fisheries of Mexico. Parasite fauna composition and their infection levels were quantified for Caranx caballus populations at 3 locations on the south central Pacific coast of Mexico. Three hundred eighty-eight fish were collected and examined between December 2009 and February 2012. Twenty-four species of parasites were identified: 2 monogeneans, 6 digeneans, 1 cestode, 5 nematodes, 8 copepods, and 2 isopods. At the component community level, species richness of parasites varied significantly from 9 to 18 (in 2011 and 2012, respectively, for Acapulco Bay), and was similar to previous reports for other species of Carangidae. The component communities and infracommunities of C. caballus exhibited a similar pattern: low species numbers, low diversity, and dominance by a single species. Multivariate discriminant analysis used to distinguish between the C. caballus populations, indicated that each location can be considered as a different fish stock, and that C. caballus, therefore, does not migrate between the studied locations, even though they are close from each other.

Resumen: Se sabe que los parásitos son útiles como marcadores biológicos en la distinción de stocks de peces, aunque el método de la parasitofauna ha recibido un uso limitado en México. Se determinó la composición y los niveles de infección de la parasitofauna en poblaciones de Caranx caballus de 3 localidades del Pacífico mexicano. Se capturaron y examinaron 388 peces entre diciembre del 2009 y febrero del 2012, identificándose 24 especies de parásitos: 2 monogéneos, 6 digéneos, un cestodo, 5 nematodos, 8 copepodos y 2 isopodos. A nivel de comunidad componente, el número de especies de parásitos varió significativamente de 9 a 18 (en el 2011 y 2012, respectivamente, para la bahía de Acapulco), siendo similar a los registros previos obtenidos para otras especies de Carangidae. Las comunidades componentes, al igual que las infracomunidades de C. caballus presentaron un patrón similar: bajo número de especies, poca diversidad y dominancia de una sola especie. Los análisis multivariados utilizados para diferenciar poblaciones de C. caballus indicaron que cada localidad puede ser considerada como un stock de peces diferente y que por lo tanto C. caballus no realiza migraciones entre las localidades estudiadas, aunque sean cercanas una de otra.

https://doi.org/10.1016/j.rmb.2016.07.010

14.

Modelación y simulación computacional del proceso de evaporación osmótica
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Forero Longas, Freddy Pulido Díaz, Adriana Patricia Cabrera Navarro, Sergio Andrés

Tecnura

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Jul 2016, Volume 20 Nº 49 Páginas 29 - 44

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Contexto: Dentro de las tecnologías de procesamiento con membranas, la evaporación osmótica es una alternativa promisoria para la transformación de frutas exóticas, generando productos concentrados que pueden ser usados en la alimentación diaria, siendo más fáciles de consumir, disminuyendo gastos de transporte e incrementando la vida útil. Método: En este trabajo de investigación se estudió y desarrollo una estrategia integral para la modelación y simulación multifísica de los fenómenos de transferencia de masa y movimiento en el proceso de evaporación osmótica, por medio del software Comsol® y Matlab®, usando un enfoque de geometría axial en dos dimensiones como simplificación del módulo real y el método de elementos finitos para la solución numérica, las simulaciones fueron validadas experimentalmente en un sistema de evaporación osmótica a escala de laboratorio. Resultados: Los modelos utilizados y las simulaciones generadas fueron estadísticamente significativas (p<0,05) en predecir el comportamiento del flux, teniendo en cuenta el efecto de la velocidad y temperatura de alimentación, junto con la velocidad de la salmuera, obteniéndose correlaciones mayores al 96% entre los datos experimentales y los calculados. Conclusiones: Se encontró que para las condiciones estudiadas el modelo difusional Knudsen es el más adecuado en describir la transferencia del vapor de agua a través de la membrana hidrófoba; las simulaciones desarrolladas describen de forma adecuada el proceso de evaporación osmótica, convirtiéndose en una herramienta para el desarrollo más rápido y económico de esta tecnología.

Context: Within the processing technologies with membranes, osmotic evaporation is a promising alternative for the transformation of exotic fruits, generating concentrated products that can be used in the daily diet, being easier to consume, reducing transportation costs and increasing shelf life. Method: In this research, it was studied and developed a comprehensive strategy for multiphysics modeling and simulation of mass and momentum transfer phenomena in the process of osmotic evaporation through Comsol® and Matlab® software. It was used an axial geometry approach in two dimensions as simplifications of real module and the finite element method for the numerical solution. The simulations were validated experimentally in an osmotic evaporation system of laboratory scale. Results: The models used and the generated simulations were statistically significant (p <0,05) in predicting the flux behavior, taking into account the effect of flow and temperature feed together with the brine flow, being obtained correlations above 96% between experimental and calculated data. Conclusions: It was found that for the conditions studied the Knudsen diffusion model is most suitable to describe the transfer of water vapor through the hydrophobic membrane. Simulations developed adequately describe the process of osmotic evaporation, becoming a tool for faster economic development of this technology.

https://doi.org/10.14483/udistrital.jour.tecnura.2016.3.a02

15.

El estudiante como protagonista de su aprendizaje: la necesidad del uso del portafolio en enfermería dentro del contexto de educación superior
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García-Carpintero Blas, Eva Siles González, José Martínez Roche, María Emilia Martínez de Miguel, Esther González Cervantes, Sergio Pulido Mendoza, Rosa

Index de Enfermería

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Jun 2015, Volume 24 Nº 1-2 Páginas 93 - 97

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

The reforms promoted by the Bologna process have led to changes in the forms of teaching and learning within the framework of European Higher Education. This brings extensive remodeling on the one hand a structural change and on the other, a re-conceptualization of the learning process supported by constructivist theories, through methodological strategies developed knowledge construction by students. The aim of this paper is to present a theoretical reflection on using the portfolio as a teaching resource for teaching and learning in this context. It has been observed that the involvement of students in identifying problems through the portfolio, provides teachers with a greater understanding of the learning processes of students as well as an opportunity for them to make a more reflective learning, independent and participatory practices eminently important in disciplines such as Nursing.

Las reformas promovidas por el proceso Bolonia han implicado cambios en las formas de enseñar y aprender dentro del marco del Espacio Europeo de Educación Superior. Esta amplia remodelación trae consigo por un lado un cambio estructural y, por otro, una reconceptualización del proceso de aprendizaje apoyada en las teorías constructivistas, a través de estrategias metodológicas de construcción del conocimiento desarrolladas por los estudiantes. El objetivo del presente artículo es presentar una reflexión teórica acerca del uso del portafolio como recurso didáctico para la enseñanza y aprendizaje dentro de este contexto. Se ha observado que la implicación de los estudiantes en la identificación de los problemas a través del portafolio, facilita a los docentes una mayor comprensión de los procesos de aprendizaje de los estudiantes, así como una oportunidad para éstos de efectuar un aprendizaje más reflexivo, autónomo y participativo, tan importante en disciplinas eminentemente prácticas como la Enfermería.

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