ABSTRACT Agroindustrial potato (Solanum tuberosum L.) waste mainly includes peel and pulp; however, potatoes of low industrial quality are also discarded by industry. A viable alternative to give added value to these byproducts is the use of these as seeds, as well as the use of starch. The objective of this work was to evaluate the physiological quality (germination and vigor), starch content and relationship between these variables in discarded tubers from the industry through chemical-structural analyses to better understand their influence on starch composition. The physiological quality of the potatoes was evaluated through germination and vigor tests; likewise, starch was extracted, and the yield was determined. Physicochemical characterization of the starches was carried out through proximal and structural analysis using nuclear magnetic resonance (NMR). The results showed that the tubers had 100.00% germination and 98.66% vigor and were suitable for use as seeds. The starch yield did not significantly differ. However, characterization of the starch revealed significant differences (p ≤ 0.05) in moisture, ash, carbohydrate and amylose contents, which were influenced by the storage temperature (4 °C) of the tubers subjected to a vigor test, which interferes with enzymatic activity. During germination, the NMR results showed type B crystallinity, which is typical of tubers, indicating that the viability tests did not promote changes in the type of crystallinity. The results obtained can help us understand the effect of germination on the composition of starch and, accordingly, choose the most appropriate applications for this starch, whether food or nonfood.

ABSTRACT Background: Osteoarthritis is a frequent rheumatic disease. Some single-nucleotide polymorphisms of the gene associated with fat mass and obesity are associated with increased body mass index and knee osteoarthritis. Objective: The objective of this study was to analyze the association of single nucleotide polymorphism rs1477196 of the fat mass and obesity gene with primary knee osteoarthritis. Methods: This observational and cross-sectional study included 347 Mexican participants. We performed the genotypification analysis with TaqMan® probe C_2031262_10 for rs1477196 (Thermo Fisher Scientific). Multivariate analysis included covariables such as age, type 2 diabetes, obesity, and postmenopause. Results: Type 2 diabetes, obesity, and postmenopause were associated with primary knee osteoarthritis in female participants. We did not find an association between rs1477196 and obesity. In the codominant and dominant genetic models, rs1477196 was significantly associated with primary knee osteoarthritis only in the female group, including in the model adjusted by other covariables (odds ratio = 2.517; 1.035-6.123; p = 0.042 and odds ratio = 2.387; 1.054-5.407; p = 0.037, respectively). The interaction between rs1477196 and obesity was significantly associated with primary knee osteoarthritis in female participants (p = 0.039 and p = 0.043). Conclusions: Our findings suggest that the rs1477196 variant of the fat and obesity mass gene may be associated with the risk of primary knee osteoarthritis in women.

Summary: Fertilization is one of the most important and costly activities in forested plant production in container. The application of controlled release fertilizers (CRF) allows reducing costs since they are incorporated in the substrate in a single event. However, the optimum dosage should be defined based on the nutritional needs of each species. Thus, the objective of the present study is to determine the effect of three CRF doses on morphological growth and in nitrogen use efficiency (NUE) in Pinus cooperi C. E. Blanco plants in the nursery. Multicote 8® [18-6-12 of nitrogen (N), phosphorus (P), and potassium (K)] was used as fertilizer in three doses: 4, 6 and 8 g L-1 of substrate. At 49 weeks after sowing, the following evaluations were performed: stem base diameter, height, aerial and root dry weight and their relationship. A leaf analysis of N, P and K was performed to determine the nutritional status of the plants by a vector analysis and estimate the NUE. The treatments were analyzed under a completely randomized design with an analysis of variance (ANOVA) and Tukey’s means test. The individuals fertilized with the 6 and 8 g L-1 dosage had better morphological attributes. The shoot-to-root ratio maintained the desirable values in all the treatments. The N, P, and K contents were higher with the 6 and 8 g L-1 doses, although NUE increased as the CRF dose decreased. The vector nomograms indicated that a high dosage does not have the effect on growth, actually toxicity may exist due to excessive fertilization. In conclusion, the 6 g L-1 CRF dose favors P. cooperi growth and nutrition in the nursery.

Resumen: La fertilización es una de las actividades más importantes y costosas en la producción de plantas forestales en contenedor. La aplicación de fertilizantes de liberación controlada (FLC) permite disminuir costos, al ser incorporados en el sustrato en un sólo evento. Sin embargo, es necesario definir la dosis óptima con base a las necesidades nutricionales de cada especie. El objetivo de este estudio fue determinar el efecto de tres dosis de FLC en el crecimiento morfológico y en la eficiencia del uso de nitrógeno (EUN) en plantas de Pinus cooperi C. E. Blanco en vivero. Como fertilizante se utilizó Multicote 8® [18-6-12 de nitrógeno (N), fósforo (P) y potasio (K)] en tres dosis: 4, 6 y 8 g L-1 de sustrato. A las 49 semanas de cultivo se evaluó el diámetro en la base del tallo, la altura, el peso seco aéreo y de la raíz, y su relación. Se realizó un análisis foliar de N, P y K para determinar el estado nutricional de las plantas mediante análisis de vectores y EUN. Los tratamientos se analizaron bajo un diseño completamente al azar con análisis de varianzas (ANOVA) y pruebas de medias de Tukey. Los individuos fertilizados con dosis de 6 y 8 g L-1 tuvieron mejores atributos morfológicos. La relación de peso seco aéreo / peso seco radicular se mantuvo en los valores deseables en todos los tratamientos. El contenido de N, P y K fue superior con las dosis de 6 y 8 g L-1, aunque la EUN se incrementó a medida que la dosis de FLC fue menor. Los nomogramas de vectores indican que una dosis alta no tiene un efecto en el crecimiento, incluso puede existir toxicidad por fertilización excesiva. Se concluye que la dosis de 6 g L-1 de FLC favorece el crecimiento y nutrición de P. cooperi en vivero.

Abstract Introduction: Acute kidney injury (AKI) occurs frequently in COVID-19 patients and is associated with greater morbidity and mortality. Knowing the risks of AKI allows for identification, prevention, and timely treatment. This study aimed to identify the risk factors associated with AKI in hospitalized patients. Methods: A descriptive, retrospective, cross-sectional, and analytical component study of adult patients hospitalized with COVID-19 from March 1 to December 31, 2020 was carried out. AKI was defined by the creatinine criteria of the KDIGO-AKI guidelines. Information, regarding risk factors, was obtained from electronic medical records. Results: Out of the 934 patients, 42.93% developed AKI, 60.59% KDIGO-1, and 9.9% required renal replacement therapy. Patients with AKI had longer hospital stay, higher mortality, and required more intensive care unit (ICU) admission, mechanical ventilation, and vasopressor support. Multivariate analysis showed that age (OR 1.03; 95% CI 1.02–1.04), male sex (OR 2.13; 95% CI 1.49–3.04), diabetes mellitus (DM) (OR 1.55; 95% CI 1.04–2.32), chronic kidney disease (CKD) (OR 2.07; 95% CI 1.06–4.04), C-reactive protein (CRP) (OR 1.02; 95% CI 1.00–1.03), ICU admission (OR 1.81; 95% CI 1.04–3.16), and vasopressor support (OR 7.46; 95% CI 3.34–16.64) were risk factors for AKI, and that bicarbonate (OR 0.89; 95% CI 0.84–0.94) and partial pressure arterial oxygen/inspired oxygen fraction index (OR 0.99; 95% CI 0.98–0.99) could be protective factors. Conclusions: A high frequency of AKI was documented in COVID-19 patients, with several predictors: age, male sex, DM, CKD, CRP, ICU admission, and vasopressor support. AKI occurred more frequently in patients with higher disease severity and was associated with higher mortality and worse outcomes.

RESUMO Introdução: Lesão renal aguda (LRA) ocorre frequentemente em pacientes com COVID-19 e associa-se a maior morbidade e mortalidade. Conhecer riscos da LRA permite a identificação, prevenção e tratamento oportuno. Este estudo teve como objetivo identificar fatores de risco associados à LRA em pacientes hospitalizados. Métodos: Realizou-se estudo descritivo, retrospectivo, transversal e de componente analítico de pacientes adultos hospitalizados com COVID-19 de 1º de março a 31 de dezembro, 2020. Definiu-se a LRA pelos critérios de creatinina das diretrizes KDIGO-LRA. Informações sobre fatores de risco foram obtidas de prontuários eletrônicos. Resultados: Dos 934 pacientes, 42,93% desenvolveram LRA, 60,59% KDIGO-1 e 9,9% necessitaram de terapia renal substitutiva. Pacientes com LRA apresentaram maior tempo de internação, maior mortalidade e necessitaram de mais internações em UTIs, ventilação mecânica e suporte vasopressor. A análise multivariada mostrou que idade (OR 1,03; IC 95% 1,02–1,04), sexo masculino (OR 2,13; IC 95% 1,49–3,04), diabetes mellitus (DM) (OR 1,55; IC 95% 1,04–2,32), doença renal crônica (DRC) (OR 2,07; IC 95% 1,06–4,04), proteína C reativa (PCR) (OR 1,02; IC 95% 1,00–1,03), admissão em UTI (OR 1,81; IC 95% 1,04–3,16) e suporte vasopressor (OR 7,46; IC 95% 3,34–16,64) foram fatores de risco para LRA, e que bicarbonato (OR 0,89; IC 95% 0,84–0,94) e índice de pressão parcial de oxigênio arterial/fração inspirada de oxigênio (OR 0,99; IC 95% 0,98–0,99) poderiam ser fatores de proteção. Conclusões: Documentou-se alta frequência de LRA em pacientes com COVID-19, com diversos preditores: idade, sexo masculino, DM, DRC, PCR, admissão em UTI e suporte vasopressor. LRA ocorreu mais frequentemente em pacientes com maior gravidade da doença e associou-se a maior mortalidade e piores desfechos.

Resumen Introducción: el hepatocarcinoma (HCC) es el tumor hepático primario maligno más frecuente en el mundo: en 2018 ocupó la sexta posición y representó la cuarta causa de muerte por cáncer; la supervivencia global a 5 años es del 18 %. La mayoría de los casos de HCC se desarrolla en pacientes con cirrosis de cualquier etiología, especialmente por virus de la hepatitis B y C, alcohol y, recientemente, por la esteatohepatitis no alcohólica (NASH). Objetivo: analizar las características clínicas, métodos de diagnóstico, tratamientos, variables pronósticas y supervivencia. Metodología: estudio descriptivo retrospectivo de una cohorte de pacientes con diagnóstico de cirrosis atendidos entre enero de 2011 y diciembre de 2020 en un centro de atención médica de Bogotá, con diagnóstico de HCC confirmado radiológicamente o por biopsia. La información se analizó de forma descriptiva con medidas de frecuencia absoluta en el caso de las variables categóricas; para las variables continuas se resumió la información con medidas de tendencia central (media o medianas) y su respectiva medida de dispersión. Resultados: se incluyeron 152 pacientes diagnosticados con HCC, con edad promedio de 69,4 años, 51,3 % eran hombres. La principal causa de HCC fue el hígado graso no alcohólico (NAFLD), que representó casi una tercera parte de los casos (32 %); otras causas fueron el alcohol (15 %) y el virus de la hepatitis C (14 %). La mediana de presentación del tumor fue de 2 nódulos con un tamaño cercano a 4 cm. El 35 % de los pacientes tenía un estadio BCLC (Barcelona Clinic Liver Cancer) con opciones curativas y el 25 % de los pacientes recibió opciones curativas de tratamiento. La terapia sistémica de primera línea utilizada en esta cohorte fue el sorafenib®, que se utilizó en 35 pacientes (33,7 %). Las curvas de supervivencia mostraron que las mujeres, el estadio Child-Pugh A y el estadio BCLC 0 presentaron mayores medianas de supervivencia. El análisis multivariado evidenció un mayor riesgo de muerte al ser hombre (Hazard ratio [HR]: 2,16; intervalo de confianza [IC]: 1,24 a 3,76), estar en los estadios Child-Pugh B (HR: 2,14; IC: 1,16 a 3,95) y Child-Pugh C (HR: 7,52; IC: 2,88 a 19,57). Conclusiones: el NAFLD es la principal causa de HCC en la presente cohorte, una tercera parte de los pacientes se diagnostica en estadios BCLC tempranos con opción curativa de tratamiento, y un 25 % se trata con terapias curativas. El sorafenib fue la terapia de primera línea en HCC avanzado. La supervivencia global luego del diagnóstico de HCC sigue siendo baja, y es necesario aunar esfuerzos en el seguimiento de los pacientes con cirrosis para mejorar estos resultados.

Abstract Introduction: Hepatocellular carcinoma (HCC) is the most frequent malignant primary liver tumor globally. In 2018, it ranked sixth and represented the fourth cause of death from cancer; the five-year overall survival is 18 %. Most cases of HCC develop in patients with cirrhosis of any etiology, especially because of hepatitis B and C viruses, alcohol, and recently nonalcoholic steatohepatitis (NASH). Aim: To analyze the clinical characteristics, diagnostic methods, treatments, prognostic variables, and survival. Materials and methods: This retrospective descriptive study was conducted on a cohort of patients diagnosed with cirrhosis and treated between January 2011 and December 2020 at a health care center in Bogotá. The diagnosis of HCC was confirmed radiologically or by biopsy. We analyzed the information descriptively with absolute frequency measures in the case of categorical variables. For continuous variables, the information was summarized with measures of central tendency (mean or median) and their relevant measures of dispersion. Results: We included 152 patients diagnosed with HCC, with a mean age of 69.4 years; 51.3 % were men. The leading cause of HCC was nonalcoholic fatty liver disease (NAFLD), which accounted for almost a third of cases (32 %); other causes were alcohol (15 %) and hepatitis C virus (14 %). The median manifestation of the tumor was two nodules with a size close to 4 cm. Besides, 35 % of patients had a BCLC (Barcelona Clinic Liver Cancer) stage with curative options, and 25 % received curative treatment options. The first-line systemic therapy used in this cohort was sorafenib®, used in 35 patients (33.7 %). Survival curves showed that women, Child-Pugh class A, and BCLC stage 0 had higher median survival. Multivariate analysis showed a higher risk of death for males (hazard ratio [HR]: 2.16; confidence interval [CI]: 1.24-3.76), Child-Pugh class B (HR: 2.14; CI 1.16-3.95), and Child-Pugh class C (HR: 7.52; CI 2.88-19.57). Conclusions: NAFLD is the leading cause of HCC in this cohort. A third of patients are diagnosed in early BCLC stages with a curative treatment option, and 25 % are treated with curative therapies. Sorafenib was the first-line therapy in advanced HCC. Overall survival after diagnosis of HCC remains low, being necessary to join forces in the follow-up of patients with cirrhosis to improve these outcomes.

RESUMEN Introducción: La periodontitis apical (PA) como enfermedad prevalente conecta la salud oral al estado general del individuo. Se pretendió caracterizar bajo un contexto epidemiológico integral la presencia de PA en una población de una clínica de Odontosánitas en Bogotá. Materiales y métodos: Este estudio transversal incluyó registro retrospectivo documental de 3981 pacientes asistentes al servicio de endodoncia. La condición periapical; ausencia o presencia de PA, fue registrada clínica y radiográficamente. Como variables sociodemográficas e individuales: sexo, edad, procedencia, tipo de aseguramiento, tipo y localización del diente. La condición médica incluyó presencia o ausencia de enfermedades sistémicas, el consumo de medicamentos y hábito de fumar. Se estimó un cálculo de 345 pacientes, con un índice de prevalencia de 43,6%. Análisis uni/bivariados, registraron la prevalencia. Un modelo multivariado de correspondencias múltiples (ACM), identificó espacialmente, la asociación y la frecuencia de los factores analizados. Resultados: Un total de 378 asistentes con una prevalencia de PA del 51,6% [IC 95% (46,5 %, 56,6%)] y 48.98 años de edad promedio. A partir de la PA, se observó mayor prevalencia en mujeres (51,8%), molares (40%) y maxilar superior (63,3%). Discusión: El ACM, identificó que la PA se distribuyó equitativamente entre las variables, sin establecer asociación entre la PA y la condición sistémica.

ABSTRACT Periapical periodontitis (PP), as a prevalent disease, connects oral health to the individual health condition. It was intended to characterize the presence of PP in a population of an Odontosánitas clinic center-Bogotá under a comprehensive epidemiological context. A Cross-sectional study that included the retrospective documentary registry of 3981 patients attending the endodontic service. The periapical condition, absence or presence of PP, was recorded clinically and radiographically asexplanatory variable. As sociodemographic and individual variables: sex, age, local area, type of insurance, and tooth. Medical condition included the presence or absence of systemic diseases, association with the type of medicine, and smoking. Calculation of 345 patients was estimated, with a prevalence rate of 43,6%. Uni / bivariate analysis recorded the prevalence. A multivariate multiple correspondence analysis (MCA) spatially identified the association and frequency of the factors analyzed.A total of 378 attendees were included, with a prevalence of PP of 51,6% [95% CI (46,5%, 56.6%)] and a mean age of 48,98 years. From the PP, it was observed higher prevalence in women (51,8%), molars (40%), and maxilla (63,3%). The MCA identified that the PP was equally distributed among the variables, without establishing an association between the PP and the systemic condition.

Resumen Introducción: la infección por coronavirus del síndrome respiratorio agudo grave de tipo 2 (SARS-CoV-2) concentra la mayor atención en el momento. En el hígado es frecuente la elevación asintomática de transaminasas y la afectación hepática varía del 14 % al 78 %. La evaluación de las comorbilidades hepáticas es escasa, con prevalencias que oscilan entre el 2 % y el 11 %. Objetivo: describir el comportamiento de una cohorte de pacientes con enfermedades hepáticas que presentaron el coronavirus de 2019 (COVID-19). Materiales y métodos: estudio observacional retrospectivo que analizó el comportamiento de una cohorte de pacientes con hepatopatías que enfermaron por COVID-19. Resultados: 543 pacientes padecieron por COVID-19, de los cuales 300 fueron mujeres (55,3 %). La mediana de edad al diagnóstico de la enfermedad hepática fue de 52 años. Las principales causas de las hepatopatías fueron esteatohepatitis no alcohólica (49,5 %), enfermedad colestásica (7,7 %), virus de la hepatitis C y B (6,3 %). La alanina-aminotransferasa (ALT) presentó una mediana de 52 U/L (rango intercuartílico [RIC]: 30-98) y aspartato-aminotransferasa (AST) 32 U/L (RIC: 23-62). La mortalidad por la infección viral fue del 5,7 % con una tasa de incidencia de 2,9 (intervalo de confianza [IC] 95 %: 2-4,2). Conclusiones: es un estudio de carácter retrospectivo; sin embargo, hasta la elaboración del manuscrito es la primera cohorte en Colombia en describir el comportamiento de las enfermedades hepáticas en pacientes que enferman de COVID-19. No se encontraron diferencias estadísticamente significativas entre las causas de hepatopatía que confieran un mayor riesgo de mortalidad; sin embargo, tener una descompensación de cirrosis es la única condición que tiene una relación con la mortalidad.

Abstract Introduction: Severe acute respiratory syndrome type 2 coronavirus infection (SARS-CoV-2) is receiving the most attention now. The asymptomatic elevation of transaminases is typical in the liver, and liver involvement varies from 14 % to 78 %. The assessment of liver comorbidities is scarce, with prevalence ranging between 2 % and 11 %. Aim: To describe the behavior of a cohort of patients with liver diseases who fell ill with coronavirus disease 2019 (COVID-19). Materials and methods: This retrospective observational study analyzed the behavior of a cohort of patients with liver diseases who fell ill with COVID-19. Results: 543 patients became ill with COVID-19, of which 300 were women (55.3 %). The median age at diagnosis of liver disease was 52 years. The leading causes of liver disease were nonalcoholic steatohepatitis (49.5 %), cholestatic disease (7.7 %), and hepatitis C and B viruses (6.3 %). Alanine aminotransferase (ALT) had a median of 52 U/L (interquartile range [IQR]: 30-98) and aspartate aminotransferase (AST) 32 U/L (IQR: 23-62). Mortality due to viral infection was 5.7 %, with an incidence rate of 2.9 (95 % confidence interval [CI]: 2-4.2). Conclusions: It is a retrospective study but, until the preparation of the manuscript, it had been the first cohort in Colombia to describe the behavior of liver diseases in patients who become ill with COVID-19. No statistically significant differences were found between the causes of liver disease that confer a higher risk of mortality; however, having decompensated cirrhosis is the only condition related to mortality.

Resumen La adición de biosólidos a suelos agrícolas puede ayudar a mejorar la calidad de dichos suelos mediante un mayor almacenamiento de C y N. Se realizó un estudio para analizar el impacto a corto plazo de la adición de biosólidos en la liberación de nutrientes y la actividad microbiana en el suelo. Se evaluó el C de la biomasa microbiana (MB-C), la actividad ureasa (UA) y la mineralización de N y C a diferentes dosis de aplicación de biosólidos (0 mg, 100 mg y 200 mg de N-NH4 + kg-1). Además, se probó un tratamiento solo con biosólidos. Se observó un aumento en la mineralización de C y N, volatilización de NH3 y contenido de MB-C, conforme a la tasa de aplicación. La UA aumentó en promedio 100% en los primeros siete días de incubación para los tratamientos de biosólidos. Estos resultados sugieren una mejora en el contenido de nutrientes y una estimulación positiva de la actividad microbiana.

Abstract Adding biosolids to agricultural soils can improve its quality through increased storage of C and N. A study to analyze the short-term impact of the addition of biosolids on the release of nutrients and the microbial activity in agricultural soil was carried out. The microbial biomass C (MB-C), urease activity (UA), and mineralization of N and C at different application rates of biosolids were evaluated (0 mg, 100mg and 200 mg of N-NH4 + kg-1). In addition, a biosolid-only treatment was tested. It was observed an increase in C and N mineralization, NH3 volatilization, and MB-C content, according to the application rate of biosolids. In biosolid treatments, UA increased 100% on average in the first seven days of incubation. These results suggest that the nutrient content in the soil is improved and microbial activity is positively stimulated.

Resumo: o vírus da imunodeficiência humana tipo 1 (HIV-1) é o agente etiológico da síndrome da imunodeficiência adquirida (AUXILIA), urna pandemia com elevados custos económicos e sociais. A glicoproteína do envelope (ENV) do vírus media o processo infeccioso ligando-se e entrando na célula hospedeira, um dos principais componentes alvo dos estudos desde sua descoberta. Seu endo domínio ou cauda C-terminal (CTT) participa de processos do ciclo replicativo tardio, como tráfego intracelular, ativação e morte celular, que ocorre porque interage com múltiplos fatores celulares por meio de motivos ou sequências-sinal presentes em toda a sua estrutura. Embora essas interações não tenham sido totalmente compreendidas em níveis específicos, estudos ao longo de mais de três décadas não deixam dúvidas de que esse domínio desempenha um papel fundamental na biologia do vírus e provavelmente no desenvolvimento da doença. Esta revisão descreve os estudos realizados até o momento que demonstram a importância da CTT, com foco nos motivos responsáveis por suas interações e seus possíveis papéis na patogenicidade da infecção.

Abstract: human immunodeficiency virus type 1 (HIV-1) is the etiological agent of acquired immunodeficiency syndrome (AIDS), a pandemic with high economic and social costs. The envelope glycoprotein (ENV) of the virus mediates the infectious process by binding to and entering the host cell, one of the main target components of studies since its discovery. Its endodomain or C-terminal tail (CTT) participates in late replicative cycle processes, such as intracellular trafficking, activation, and cell death, which occurs because it interacts with multiple cellular factors through motifs or signal sequences present throughout its structure. Although these interactions have not been fully understood at specific levels, studies over more than three decades leave no doubtthatthis domain plays a fundamental role in the biology of the virus and probably the development of the disease. This review describes the studies carried out to date that demonstrate the importance of the CTT, focusing on the motifs responsible for its interactions and its possible roles in the pathogenicity of the infection.

Resumen: el virus de la inmunodeficiencia humana tipo 1 (VIH-1) es el agente etiológico del síndrome de inmunodeficiencia adguirida (SIDA), una pandemia con altos costos económicos y sociales. La glicoproteína de la envoltura (ENV) del virus media el proceso infeccioso al unirse a la célula huésped y entrar en ella, uno de los principales componentes objetivo de los estudios desde su descubrimiento. Su endodominio o cola C-terminal (CTT) participa en procesos tardíos del ciclo replicativo, como tráfico intracelular, activación y muerte celular, lo que ocurre porque interactúa con múltiples factores celulares a través de motivos o secuencias señal presentes en toda su estructura. Aunque estas interacciones no se han entendido completamente a niveles específicos, los estudios durante más de tres décadas no dejan dudas de que este campo juega un papel fundamental en la biología del virus y probablemente en el desarrollo de la enfermedad. Esta revisión describe los estudios realizados hasta la fecha que demuestran la importancia de la CTT, centrándose en los motivos responsables de sus interacciones y sus posibles roles en la patogenicidad de la infección.

Resumen Introducción: Las alteraciones de la bioquímica hepática son frecuentes en los pacientes con infección por VIH, la etiología es variada, la esteatosis hepática es frecuente con una prevalencia estimada del 60% Objetivos: Caracterizar las alteraciones hepáticas en una serie de pacientes con infección por VIH en un centro de investigación de Bogotá Colombia durante el periodo 2009 - 2019. Materiales y Métodos: Estudio descriptivo, retrospectivo, observacional de pacientes con infección por VIH que asistieron a un centro de investigación durante los años 2009-2019. Resultados: 94% fueron hombres y 6% mujeres con edad promedio de 44 años, 92,5% de los pacientes presentaba uso de terapia antiretroviral. Las principales hepatopatías fueron la coinfección VIH-Hepatitis C y el hígado graso en iguales porcentajes, 31,3%. El promedio del indice HOMA fue de 2,58. Discusión: Las enfermedades hepáticas son una causa importante de morbimortalidad en pacientes con infección por VIH, las coinfecciones virales y el hígado graso pueden ser muy frecuentes en nuestro medio a diferencia de otros estudios Conclusiones: Este es el primer estudio a nivel local en describir las alteraciones hepáticas en pacientes con VIH, las comorbilidades no SIDA, juegan un papel importante dentro de la enfermedad. La hepatitis C continúa siendo una coinfección frecuente en la población VIH.

Abstract Introduction: Alterations in liver biochemistry are frequent in patients with HIV infection, the etiology is varied and includes multiple causes, liver steatosis is one of the most frequent with an estimated prevalence of 60% after the appearance of antiretroviral treatment Objectives: To characterize liver disorders in a series of patients with HIV infection at a research center in Bogotá Colombia during the period 2009-2019. Materials and Methods: Descriptive, retrospective, observational study of patients with HIV infection who attended a disease research center during the years 2009-2019. Results: 67 clinical histories were reviewed, 94% were men and 6% women with an average age of 44 years, 92.5% of the patients had use of anti-retroviral therapy and the diagnosis of HIV was known 11.7 years ago on average. The main liver diseases were HIV-Hepatitis C coinfection and fatty liver in equal percentages, 31.3%. The average HOMA index was 2.58. Discussion: Liver diseases are an important cause of morbidity and mortality in patients with HIV infection. Viral coinfections and fatty liver can be very frequent in our setting, unlike other studies. Conclusions: This is the first study locally to describe the liver disorders in patients with HIV, non-AIDS comorbidities, including fatty liver, play an important role in the disease and could behave like the general population. Hepatitis C continues to be a frequent coinfection in the HIV population.

ABSTRACT: The glass ionomer cements (GICs) is a generic name given to a group of materials widely used in clinical dentistry which if used after the specified expiration date, material properties may be affected. to evaluate the Vick ers microhardness, surface morphology and the energy dispersive X-ray microanalysis (EDX), of GICs with different expiration dates that were stored at room temperature. specimens of highly viscous glass ionomer cement (HVGIC) (Ketac Cem and Ketac Molar) and resin-modified glass ionomer cement (RMGIC) (Vitrebond) with different expiration dates (current, close to their expiration and expired) were prepared for Vickers microhardness test and scanning electronic microscopy (SEM) with EDX, measuring 5 mm in diameter and 2 mm length, per the manufacturer’s instructions, in standard cylindrical teflon molds. For the comparison of obtained values, the ANOVA test was used, while Tukey test was used for the multiple comparisons. In all the GICs used, the microhardness decreased as the expiration date approached, finding a significant statistical differen ce (P<0.05) in Ketac Molar and Vitrebond. SEM sample analysis revealed similar cohesive cracks in all tested materials. The EDX analysis revealed the presence of the elements F, Al and Si in all GICs and Ca only in Ketac Molar and Ketac Cem. The elements were found in a higher atomic percentage in the GICs with an current date and in a lower percentage in those with an expired date. HVGIC and RMGIC with an expiration date finish and that were stored at room temperature, suffer significant physical and chemical changes, which could put doubts its clinical effectiveness.

RESUMEN: El cemento de ionómero de vidrio (CIV) es un nombre genérico que se le da a un grupo de materiales ampliamente utilizados en odontología clínica que si se usan después de la fecha de vencimiento especificada, las propiedades del material pueden verse afectadas. evaluar la microdureza Vickers, la morfología superficial y el microanálisis de energía dispersa de rayos X (EDX), de CIV con diferentes fechas de caducidad almacenados a temperatura ambiente. Muestras de cemento de ionómero de vidrio de alta viscosidad (CIVAV) (Ketac Cem y Ketac Molar) y cemento de ionómero de vidrio modificado con resina (CIVMR) (Vitrebond) con diferentes fechas de vencimiento (vigente, próximo a su vencimiento y vencido) de 5 x 2 mm, fueron preparadas para microscopía electrónica de barrido (MEB) con EDX y microdureza Vickers, según las instrucciones del fabricante, en moldes de teflón cilíndricos estándar. Para la comparación de los valores obtenidos se utilizó la prueba ANOVA, mientras que para las comparaciones múltiples se utilizó la prueba de Tukey. En todos los CIV utilizados, la microdureza disminuyó a medida que se acercaba la fecha de vencimiento, encontrándose una diferencia estadística significativa (P <0.05) en Ketac Molar y Vitrebond. El análisis de la muestra en MEB reveló grietas cohesivas similares en todos los materiales probados. El análisis EDX reveló la presencia de los elementos F, Al y Si en todos los GIC y Ca solo en Ketac Molar y Ketac Cem. Los elementos se encontraron en mayor porcentaje atómico en los GIC con fecha vigente y en menor porcentaje en aquellos con una fecha vencida. Los CIVAV y CIVMR con fecha de caducidad vencida y que fueron alma- cenados a temperatura ambiente, sufrieron cambios físicos y químicos importantes, lo que podría poner en duda su efectividad clínica.

EXTENDED ABSTRACT Fecal pellets and cysts of the brine shrimp Artemia (Crustacea, Branchiopoda, Anostraca) constitute an important component of the biogenic carbonate sedimentation in many saline and hypersaline lakes bodies. However, in Argentina there are not studies of its presence in the fossil record, being this paradoxical given the abundance of large and diverse endorheic salt basins existing in the country. In this study we present the morphological and petrographic characteristics of fecal pellets and cysts of Artemia sp. and its association with evaporitic minerals occurring in a sedimentary core covering the last ca.1300 years (1220 years cal. BP) from the Chasicó Lake (38 ° 37 ‘S; 63 ° 05’ W) (Fig. 1) discussing the potential of these indicators in paleoenvironmental studies. Taking into account the ecological requirements of this crustacean, the abundance of fecal pellets and cyst in the studied deposits is discussed in relation to the physical-chemical changes that occurred during that time. The sedimentary section was carried out considering the changes of texture, structure and color of the layers to select 18 sampling intervals (Fig. 2). The qualitative determination of the total sample was performed by X-ray powder diffraction analysis. The fecal pellets are white to dry light gray and are presented in elongated cylindrical sections (Fig. 3), with one end straight and another in blunt point. They have a maximum average length of 680 μm (Fig. 4; Table 1) with a rounded net edge. They consist of a carbonaceous sludge, with a darker core of organic matter and clay, an edge with an outer shell of authigenic carbonate in very small crystals (Fig. 5). In the impregnated sections, light and dark sheets were differentiated. In light sheets, pellets with ovoidal and elongated shapes are concentrated. In contrast, in the dark layers, the pellets are presented in a smaller proportion and are distributed in a weave of gray fibers of organic matter that would correspond to a microbial mat. The cysts are reddish with an average size of 232.36 ± 8.7 μm (Table 1, Fig. 3) and are entire, broken (open) and invaginated. A succession of siliciclastic mud (mostly formed by extrabasinal clastic components) and carbonaceous peloidal micritic-aragonitic mud with evaporites minerals (mostly intrabasinal chemical and biochemical components) was recognized. The sequence was divided into four units (Fig. 2): (1) Unit C: grayish green (5Y 3/1; 5 and 4/2, 5Y 5/4) laminated and disturbed micritic-aragonitic mud with halite and thenardite (76-143 cm); (2) Unit B: gray (10YR 6/1 and 10YR 4/2) disturbed massive micritic-aragonitic mud with thenardite (Na2SO4) (57-76 cm); (3) Unit A: gray (10YR 5/1) micritic-aragonitic laminates mud with halite and thenardite subordinate (20-57 cm); (4) Unit H: gray (10YR 5/1) siliciclastic and micritic massive mud with halite (0- 20 cm) (Fig. 2). Artemia sp. fecal pellets and cysts were registered between ca. 730 AD and 1978 AD and are absent after ca. 1978 AD in the lacustrine deposits of Chasicó Lake. The sedimentary section (Fig. 2) showed a predominance of biochemical and chemical deposition and very little terrigenous material between ca. 730 AD and 1411 AD (units B and C), suggesting that the accumulation would have occurred far from the coastal margins and at depths equal to or greater than 3 m (Last, 1994). The increase of terrigenous material in some samples and the presence of lamination in unit A (subunit A1) (between ca. 1411 AD and 1978 AD) would also support that the deposition of this unit occurred in the central areas of the lake and would indicate the absence of both bottom currents (Eardley and Gvosdetsky, 1960) and seasonality in the deposition, with intervals with greater extra-basinal contribution. From ca. 1978 AD (unit H) there is an increase in the extra-basin contribution coinciding with the greater contributions from the Arroyo Chasicó water basin. The differences in mineralogy of evaporitic species associated with the presence of fecal pellets and cysts of Artemia sp. in units A, B and C, and the absence of pellets and cysts in unit H allowed recognizing four different stages that suggest net changes in the chemical environment of the lake during the last 1300 years (Fig. 2). Between ca. 730 AD and 1233 AD (unit C) there was a greater proportion of fecal pellets and cysts associated with halite and thenardite, the later as a stable phase of the mirabilite. This mineralogical association would reflect periods with high salinities (extreme hypersaline environments) that allowed the formation of halite and intervals of lower salinity (hypersaline environments) with deposition of sodium sulfate. Between ca. 1233 AD and 1411 AD (unit B) the presence of free thenardite from other salts suggests the formation of mirabilite as primary sulfate, which occurred during cold periods and salinity below the saturation index of halite. Deposits with thenardite, as the only evaporitic mineral, suggest lower salinities than in unit C that would have occurred during or after its deposition, which prevented the precipitation of chlorides. Coincidentally, a lower representation of Artemia sp. cysts was observed. From ca. 1411 AD to 1978 AD (unit A) the sequence presents sectors with lamination and abundant of fecal pellets of Artemia sp. and very thin to thin stratification (subunit A1, Fig. 2). The presence of halite suggests a significant increase in salinity over the entire period that allowed the sulfate to be in solution, or that its deposition occurred only in the form of mirabilite in coastal areas by cooling during winters. Between ca. 1978 AD and 2010 AD (unit H) fecal pellets and cysts of Artemia sp. are absent and halite is the only evaporitic mineral. This apparent contradiction would be explained by the formation of interstitial brine and an intrasedimentary precipitation of chloride, under current salinity conditions and not the salinity that corresponds to the deposition of muds. The absence of Artemia sp. was related to a reduction in salinity and the entry of predators to the lake, such as the pejerrey (Odontesthes bonariensis) that created unfavorable conditions for the reproduction and proliferation of Artemia sp. The presence and/or absence of fecal pellets and cysts of Artemia sp. showed a positive correlation with the increase and/or decrease in salinity in the lake with respect to the estimated values from precipitated salts. Inferences for the 20th century are supported by the historical and instrumental data from the Chasicó Lake. Taking into account that the cyst size is a biometric characteristic of the Artemia sp. species, this measure was used to assign the fossil cysts to A. persimilis, although specific determinations are necessary to ensure it. The presence of this native species before ca. 1978 AD and its subsequent absence suggests ecological reasons related to the effects of climate change that occurred since the end of 1970s and raises questions about the current biogeographic distribution of Artemia sp. in Argentina in relation to these changes.

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SUMMARY The Military Institute of Preventive Medicine for the Defence (IMPDEF) has played an important role during Balmis Operation in preventive medicine and public health in the field of the Armed Forces. The aim has been to provide guidance for case surveillance of COVID-19 and the adoption of measures related with force health protection, maintenance of force operativity and provide information as part of medical education. This article aims to present the role of the IMPDEF during the Balmis Operation providing recommendations for public health policy and interventions in the units in Spain and for the deployed forces.

RESUMEN El Instituto de Medicina Preventiva de la Defensa (IMPDEF) ha jugado un papel importante durante la Operación Balmis en materia de medicina preventiva y salud pública en el ámbito de las Fuerzas Armadas. La finalidad ha sido proporcionar una guía para la vigilancia de casos COVID-19 y adopción de medidas de protección sanitaria de la fuerza, mantener su operatividad y proporcionar información como parte de promoción de la salud. El presente artículo pretende dar a conocer la gestión del COVID-19 realizada desde el IMPDEF en el entorno extrahospitalario de territorio nacional y de las operaciones militares en el exterior, y su evolución durante la Operación Balmis.

Resumen El propósito de este estudio fue describir las prácticas de liderazgo que percibe un grupo de 145 empleados respecto a su jefe inmediato y estimar si éstas guardan relación con su comportamiento innovador en el trabajo. El estudio se realizó en una institución de educación superior con funcionarios del área administrativa que pertenecían al mismo departamento. Para esto, se utilizó la Escala de Liderazgo para la Innovación (Innovational Leadership Scale [ils-15]) de Contreras, Espinosa y Dornberger (2019) y la Escala de Comportamiento Innovador en el Trabajo de Janssen (2000). Ambos cuestionarios mostraron altos niveles de confiabilidad (coeficientes alfa de Cronbach superiores a .90). De acuerdo con los resultados, se observa un nivel medio de desarrollo en las dos variables estudiadas. Así mismo, se observó que los empleados con mayor comportamiento innovador perciben un mayor liderazgo para la innovación en sus jefes. Estos hallazgos contribuyen a la formación de líderes que fomenten la innovación en las organizaciones y muestran la necesidad de continuar estudiando este estilo de liderazgo en las empresas colombianas en relación con el comportamiento innovador de sus trabajadores a fin de contribuir al desarrollo de organizaciones más adaptables a las circunstancias cambiantes del entorno.

Abstract The purpose of this study was to describe the leadership practices perceived by a group of 145 employees with regard to their immediate boss and to estimate whether these practices are related to their innovative behavior at work. The study was conducted in a higher education institution with employees from the administrative area who belonged to the same department. To this end, the Innovational Leadership Scale (ils-15]) of Contreras, Espinosa, and Dornberger (2019) and the Janssen Innovative Work Behaviour Scale (2000) were used. Both questionnaires showed high levels of reliability (Cronbach’s alpha coefficients greater than .90). According to the results, an average level of development may observed in the two variables that were studied. Likewise, it was observed that the employees with greater innovative behavior perceive greater leadership for innovation in their bosses. These findings contribute to the training of leaders that foster innovation in organizations and demonstrate the need to continue studying this style of leadership in Colombian companies with regard to innovative behavior among their staff, so as to contribute to the development of organizations more adaptable to the changing circumstances of the environment.

ABSTRACT Introduction: The tolerance test of mixed food is considered the gold standard for the measurement of endogenous insulin production in patients with diabetes type 1. Objective: To determine the usefulness of the tolerance test of mixed food with Nutrial I to assess the β-cells function in patients with diabetes type 1 of recent diagnosis and the relation of this function with some clinical and biochemical characteristics. Methods: There were studied biochemical variables as the blood glucose, glycosylated haemoglobin (HbA1c), C-peptide and lipid fractions. The tolerance test of mixed food with Nutrial I was applied to 18 individuals with diabetes type 1 of recent diagnosis and in 8 volunteers aged between 19 and 35 years old. The consumption of Nutrial I supplement was calculated according to the weight of the patient. Samples were obtained for blood glucose and C-peptide at -10, 0, 30, 60, 90 and 120 minutes. Results: There were observed high concentrations of glycemia and decreased amounts of C-peptide during the tolerance test of mixed food in recently diagnosed type 1 diabetics in comparison with the volunteers, as well as differences in areas under the curve of C-peptide (AUC-pc) (p= 0.001). In the recently diagnosed type 1 diabetics was evident a negative correlation between the AUC-pc with fasting plasma glucose levels (r= -0,747; p(0.0001) and HbA1c (r= -0,535; p= 0.022). On the contrary, it was found a positive correlation between the AUC-pc and fasting C-peptide (r = 0.722; p = 0.001). The AUC-pc after the tolerance test of mixed food was greater in subjects with fasting blood glucose < 7 mmol/L with respect to the subjects with fasting blood glucose ( 7 mmol/L (p= 0.012). Conclusions: The use of Nutrial I in the tolerance test of mixed food was useful in the assessment of the role of the β-cells in patients with recently diagnosed diabetes type 1. Low values of fasting blood glucose during this test are indirect markers of a residual function of (cells more preserved in type 1 diabetics of recent diagnosis.

RESUMEN Introducción: La prueba de tolerancia de comida mixta es considerada la prueba de oro para la medición de la producción de insulina endógena en pacientes con diabetes tipo 1. Objetivo: Determinar la utilidad de la prueba de tolerancia de comida mixta con Nutrial I para evaluar la función de las células β en diabéticos tipo 1 de diagnóstico reciente y la relación de esa función con algunas características clínicas y bioquímicas. Métodos: Se estudiaron variables bioquímicas como la glucemia, hemoglobina glucosilada (HbA1c), péptido C y fracciones lipídicas. La prueba de tolerancia de comida mixta con Nutrial I se aplicó a 18 sujetos con diabetes tipo 1 de diagnóstico reciente y a 8 voluntarios con edades comprendidas entre 19 y 35 años. El consumo del suplemento Nutrial I se calculó según el peso del paciente. Se obtuvieron muestras para glucemia y péptido C a los -10, 0, 30, 60, 90 y 120 minutos. Resultados: Se observaron concentraciones elevadas de glucemia y disminuidas de péptido C durante la prueba de tolerancia de comida mixta en los diabéticos tipo 1 de diagnóstico reciente, en comparación con los voluntarios, así como, diferencias en las áreas bajo la curva de péptido C (AUC-pc) (p= 0,001). En los diabéticos tipo 1 de diagnóstico reciente se evidenció una correlación negativa entre el AUC-pc con los niveles de glucemia en ayunas (r= -0,747; p ( 0,0001) y la HbA1c (r= -0,535; p= 0,022). Por el contrario, se encontró una correlación positiva entre el AUC-pc y el péptido C en ayunas (r= 0,722; p= 0,001). El AUC-pc después de la prueba de tolerancia de comida mixta es mayor en los sujetos con glucemia en ayunas si GA < 7 mmol/L con respecto a los sujetos con glucemia en ayunas ( 7 mmol/L (p= 0,012). Conclusiones: El empleo del Nutrial I en la prueba de tolerancia de comida mixta fue útil en la evaluación de la función de las células β en diabéticos tipo 1 de diagnóstico reciente. Los valores bajos de glucemia en ayunas durante esta prueba son marcadores indirectos de una función residual de células ( más conservada en los diabéticos tipo 1 de diagnóstico reciente.