Various beneficial biological activities have been reported for passion fruit (Passiflora edulis), grown in tropical and subtropical regions. However, no study has yet demonstrated its action against the Zika virus (ZIKV) infection. The present study aimed to investigate the anti-ZIKV of the ethanolic passion fruit seed extract (PFSE), from which, liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis allowed to putatively annotate the occurrence of piceatannol, astringin, scirpusin A, scirpusin B, isookanin-7-O-glucoside and naringenin-7-O-glucoside, two quadranguloside derivatives, tyrosine, and phenylalanine. PFSE is well tolerated by cell lines (Vero E6 and HTR-8/SVneo) and tissue explants from the human placenta. With 1- and 24-h treatments, PFSE consistently reduced the viral load and NS1 protein expression of the two strains of ZIKV (MR766 and PE243) in placental explants and cell lines. Thus, PFSE has a promising potential for developing anti-ZIKV treatments, protecting the placenta against infection caused by ZIKV, along with relevant antioxidant potential, represented by ferric reducing antioxidant power (FRAP) of 390.5 Trolox eq per g dry extract and half maximal inhibitory concentration (IC50) for 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) of 12.1 µg mL-1, and more than 90% protection from lipid peroxidation. Although further studies need to be conducted, PFSE treatment was safe and effective in trophoblast cells and placental explants, thus representing a promising class of all-accessible products based on the reuse of industrial by-products.

Zonas costeiras são sistemas naturalmente dinâmicos e móveis, expostos a fatores naturais (fluxos de rios, ondas e tempestades) e intervenções humanas, que alteram continuamente a sua morfologia. Fenómenos de erosão relacionados com temporais e escassez de sedimentos são comuns. Eles ameaçam construções e infraestruturas, praias, ecossistemas e zonas húmidas, o que constitui um desafio para a segurança e defesa costeiras. Uma monitorização regular de áreas costeiras, com avaliação da sua morfodinâmica e identificação dos processos que influenciam o transporte de sedimentos, visando uma melhor compreensão das alterações e tendências evolutivas nos sistemas costeiros, torna-se portanto cada vez mais importante. Para tal necessita-se de uma abordagem multidisciplinar e investigadores especializados em processos costeiros e tecnologias de observação de ponta. O presente trabalho descreve e avalia métodos de monitorização de última geração para uma quantificação eficiente de alterações em ambientes costeiros e apresenta dois estudos de caso Portugueses. Os métodos de observação incluem: levantamentos topográficos terrestres em modo cinemático baseados em videogrametria; levantamentos topográficos aéreos baseados em fotogrametria; batimetria sub-tidal e imagens de fundo obtidas com sonar a partir de um veículo autónomo de superfície; e observações de campo com análise de sedimentos e caracterização de praias. O primeiro caso de estudo refere-se à análise de padrões de erosão e acreção na restinga do estuário do Douro, tendo em conta efeitos causados pelo molhe, o caudal do rio, ondas e vento. Antes da construção de um molhe destacado, a morfodinâmica da restinga estava relacionada com eventos extremos de caudal, agitação marítima e vento. Após a construção, a forma da restinga estabilizou e observou-se um aumento da sua área e do seu volume. No segundo caso de estudo, a costa de Vila Nova de Gaia foi amplamente estudada, incluindo shoreface, foreshore e dunas, com a caracterização dos principais atributos e uma análise de curto-período da dinâmica instalada. Os resultados obtidos a partir de dados de campo, dos levantamentos topográficos e de modelos numéricos de ondas foram combinados numa análise de risco com métodos especificamente desenvolvidos para a área de estudo. Ambos os programas de monitorização atingiram os seus objetivos e geraram informação relevante para as autoridades locais. A informação recolhida e processada constitui uma base de dados valiosa para o planeamento costeiro e a Gestão Integrada de Zonas Costeiras. Os estudos demonstram o potencial das diversas abordagens, apoiadas por tecnologias avançadas, para o estudo dos processos complexos de morfodinâmica costeira.

Coastal zones are naturally dynamic and mobile systems exposed to natural factors (river flows, waves and storms) as well as human interventions that continuously reshape their morphology. Erosion phenomena related to extreme weather events and sediment scarcity are common, threatening buildings and infrastructures, as well as beaches, ecosystems and valuable wetland; conditions that pose challenges to coastal security and defence. Regular monitoring of coastal areas, assessment of their morphodynamics and identification of the processes influencing sediment transport are thus increasingly important for a better understanding of changes and evolutionary trends in coastal systems. This demands a multi-disciplinary approach involving researchers with expertise in coastal processes and state- of-the-art observation technologies. In this paper state-of-the-art surveying methods for an efficient quantification of changes in coastal environments are described and evaluated, and two NW-Portuguese case studies are presented. Survey methods included: topographic surveys based on terrestrial videogrammetric mobile mapping and aerial photogrammetry; sub-tidal bathymetry with sonar imagery using an Autonomous Surface Vehicle (ASV); as well as field observations, with sediment sampling and beach characterisation. In the first case study, erosion/accretion patterns in the Douro estuary sand spit were analysed, considering its breakwater, river flow, wave and wind effects. Prior to the construction of a detached breakwater, the spit’s morphodynamics was related to extreme river flow events, wave and wind conditions; afterwards the spit stabilized its shape and increased its area and volume. In the second case study the coast of Vila Nova de Gaia was broadly analysed, including the shoreface, foreshore and dunes, the characterization of major features and a short-period analysis of installed dynamics. Results obtained from field data, topographical surveys and numerical wave models were combined for an erosion risk assessment, using a methodology specifically developed for the study area. Both monitoring programs achieved their proposed objectives and provided valuable information to the local authorities, as gathered and processed information constitutes a valuable database for coastal planning and for ICZM purposes. They demonstrate the potential of several approaches, supported by advanced technologies, for the study of complex coastal morphodynamic processes.

Alkaline phosphatase (ALP) is important in calcification and its expression seems to be associated with the inflammatory process. We investigated the in vitro acute effects of compounds used for the prevention or treatment of cardiovascular diseases on total ALP activity from male Wistar rat heart homogenate. ALP activity was determined by quantifying, at 410 nm, the p-nitrophenol released from p-nitrophenylphosphate (substrate in Tris buffer, pH 10.4). Using specific inhibitors of ALP activity and the reverse transcription-polymerase chain reaction, we showed that the rat heart had high ALP activity (31.73 ± 3.43 nmol p-nitrophenol·mg protein-1·min-1): mainly tissue-nonspecific ALP but also tissue-specific intestinal ALP type II. Both ALP isoenzymes presented myocardial localization (striated pattern) by immunofluorescence. ALP was inhibited a) strongly by 0.5 mM levamisole, 2 mM theophylline and 2 mM aspirin (91, 77 and 84%, respectively) and b) less strongly by 2 mM L-phenylalanine, 100 mL polyphenol-rich beverages and 0.5 mM progesterone (24, 21 to 29 and 11%, respectively). β-estradiol and caffeine (0.5 and 2 mM) had no effect; 0.5 mM simvastatin and 2 mM atenolol activated ALP (32 and 36%, respectively). Propranolol (2 mM) tended to activate ALP activity and corticosterone activated (18%) and inhibited (13%) (0.5 and 2 mM, respectively). We report, for the first time, that the rat heart expresses intestinal ALP type II and has high total ALP activity. ALP activity was inhibited by compounds used in the prevention of cardiovascular pathology. ALP manipulation in vivo may constitute an additional target for intervention in cardiovascular diseases.