Resumo Fundamento: A síndrome do PRKAG2 tipicamente se manifesta na adolescência e início da idade adulta, cursando com hipertrofia ventricular esquerda, arritmias e risco de morte súbita. O achado de marcadores ecocardiográficos antes da manifestação clínica nos filhos de pais acometidos pela doença pode facilitar a estratégia de prevenção e planejamento terapêutico para esse grupo de pacientes. Objetivo: Identificar a existência de achados ecocardiográficos que se manifestem precocemente nos filhos de pais acometidos por síndrome do PRKAG2, enquanto ainda assintomáticos. Métodos: Estudo observacional transversal em que sete participantes, filhos de pais com diagnóstico estabelecido de síndrome do PRKAG2, com idades entre 9 meses e 12 anos e diagnóstico genético comprovado, foram submetidos à ecocardiografia convencional e por técnicas avançadas, tendo seus achados comparados aos de grupo controle composto por sete voluntários pareados por sexo e idade, hígidos do ponto de vista cardiovascular. Um valor de p < 0,05 foi considerado significante. Resultados: A ecocardiografia convencional mostrou valores aumentados com significância estatística no grupo caso para átrio esquerdo, septo interventricular, parede posterior do ventrículo esquerdo, massa ventricular indexada e espessura relativa da parede (p < 0,05). O strain sistólico longitudinal global obtido pelo ecocardiograma bidimensional não mostrou diferença estatisticamente significativa entre os grupos caso e controle. Nenhum dos parâmetros ao ecocardiograma tridimensional apresentou significância estatística entre os grupos. Conclusão: Crianças diagnosticadas com PRKAG2 demonstraram achados ecocardiográficos indicativos de tendência à hipertrofia cardíaca. A ecocardiografia pode ser uma ferramenta útil na avaliação e seguimento desse grupo de pacientes, antes do início de manifestações clínicas. Fundamento PRKAG adulta esquerda súbita pacientes Objetivo assintomáticos Métodos participantes 1 comprovado avançadas cardiovascular 005 0 05 0,0 significante Resultados esquerdo interventricular 0,05. . 0,05) Conclusão cardíaca clínicas 00 0,

Abstract Background: PRKAG2 syndrome typically manifests in adolescence and early adulthood, progressing with left ventricular hypertrophy, arrhythmias, and risk of sudden death. Findings of echocardiographic markers before clinical manifestation in children of patients affected by the disease can facilitate prevention strategies and therapeutic planning for this patient group. Objective: To identify the existence of echocardiographic findings that manifest early in children of parents affected by PRKAG2 syndrome, while they are still asymptomatic. Methods: In this cross-sectional observational study, 7 participants who were children of parents with established diagnosis of PRKAG2 syndrome, between the ages of 9 months and 12 years, with proven genetic diagnosis, underwent conventional and advanced echocardiography. Their findings were compared to those of a control group composed of 7 age- and sex-matched volunteers who were healthy from a cardiovascular point of view. P values < 0.05 were considered significant. Results: Conventional echocardiography showed statistically significantly higher values in the case group for left atrium, interventricular septum, left ventricular posterior wall, indexed ventricular mass, and relative wall thickness (p < 0.05). Global longitudinal systolic strain on 2-dimensional echocardiography did not show statistical significance between the case and control groups. None of the parameters on 3-dimensional echocardiography showed statistical significance between groups. Conclusion: Children diagnosed with PRKAG2 showed echocardiographic findings indicative of a tendency toward cardiac hypertrophy. Echocardiography can be a useful tool in the evaluation and follow-up of this patient group before the onset of clinical manifestations. Background PRKAG adulthood hypertrophy arrhythmias death Objective asymptomatic Methods crosssectional cross sectional study 1 years age sexmatched sex matched view 005 0 05 0.0 significant Results atrium septum mass p 0.05. . 0.05) 2dimensional dimensional 2 groups 3dimensional 3 Conclusion followup follow up manifestations 00 0.

Abstract Objective To investigate the epidemiology of injury types among jiu-jitsu practitioners, as well as the incidence regarding different skill and experience levels, through the question: "What are the characteristics and prevalence of musculoskeletal injuries in Jiu-Jitsu practitioners?". Methods Since the beginning of the study, in August 2020, we conducted a search on the MEDLINE, LILACS, and SciELO electronic databases. We included cross-sectional studies published between 2018 and 2023 on the epidemiology of the types of injuries among jiu-jitsu practitioners that compared their incidence regarding different levels of ability and experience. Two independent researchers performed the data extraction and assessed the risk of bias. Results Seven studies were included. The common outcomes involved 2,847 jiu-jitsu practitioners. A high prevalence in the knee joint and chest and rib areas was reported. Considering the difference in experience level among the practitioners, we could observe that most of the individuals included were beginners. Among the age groups observed, male practitioners older than 30 years of age were the ones who presented the highest rate of musculoskeletal injury, especially during training sessions. Conclusion There is a high prevalence of musculoskeletal injuries among jiu-jitsu practitioners. The most affected anatomical segments are the knee joint, the chest, and the rib region, followed by the shoulder joint. The related factors change according to certain variables, being more common during training in male individuals over 30 years of age and beginners in the sport. jiujitsu jiu jitsu question What JiuJitsu Jiu Jitsu . practitioners?" study 2020 MEDLINE LILACS databases crosssectional cross sectional 201 202 bias 2847 2 847 2,84 reported observed 3 sessions region variables sport practitioners? 20 284 84 2,8 28 8 2,

Resumo Objetivo Investigar a epidemiologia dos tipos de lesões entre praticantes de jiu-jitsu e sua incidência em diferentes níveis de habilidade e experiência por meio da questão: "Quais as características e a prevalência das lesões musculoesqueléticas em praticantes de jiu-jitsu?" Métodos Desde o início do estudo, em agosto de 2020, foram pesquisados os bancos de dados MEDLINE, LILACS e SciELO. Foram incluídos estudos transversais, publicados entre 2018 e 2023, que investigaram a epidemiologia dos tipos de lesões ocorridas entre praticantes de jiu-jitsu e compararam sua incidência em diferentes níveis de habilidade e experiência. Para tanto, dois pesquisadores independentes realizaram a extração dos dados e avaliaram o risco de viés. Resultados Sete estudos foram incluídos. Os resultados comuns envolveram 2.847 praticantes de jiu-jitsu. Houve uma alta prevalência de lesão na articulação do joelho e nas áreas do tórax e das costelas. Considerando a diferença de nível de experiência entre os praticantes, pôde-se observar que a maioria dos indivíduos incluídos eram iniciantes. Entre as faixas etárias observadas, homens acima de 30 anos de idade foram os que mais apresentaram lesões musculoesqueléticas, principalmente durante os treinos. Conclusão Houve uma alta prevalência de lesões musculoesqueléticas entre os praticantes de jiu-jitsu. Os segmentos anatômicos mais acometidos foram a articulação do joelho, o tórax e a região das costelas, seguidos da articulação do ombro. Os fatores relacionados mudaram de acordo com algumas variáveis, sendo mais comuns durante o treinamento em indivíduos do sexo masculino com mais de 30 anos e iniciantes na modalidade. jiujitsu jiu jitsu questão Quais jiu-jitsu? estudo 2020 MEDLINE SciELO transversais 201 2023 tanto viés 2847 2 847 2.84 jiujitsu. jitsu. costelas pôdese pôde se observadas 3 treinos ombro variáveis modalidade 202 20 284 84 2.8 28 8 2.

Resumo Objetivou-se realizar uma análise espacial da taxa de mortalidade hospitalar (TMH) por síndrome respiratória aguda grave (SRAG) atribuída à COVID-19 em crianças e adolescentes no Brasil no período de 2020 a 2021. Utilizou-se o método de cluster para agrupar as unidades federativas (UFs) com base na TMH. Em 2020, clusters com altas TMHs foram formados por UFs Norte/Nordeste. Em 2021, houve redução na TMH. Os clusters com maiores taxas permaneceram na região N/NE. Diferenças regionais foram observadas nas TMHs. Os achados podem refletir as desigualdades sociais e o acesso à atenção hospitalar, principalmente na faixa etária de menores de 1 ano pela gravidade da doença neste grupo.

Abstract The objective was to perform a spatial analysis of the hospital mortality rate (HMR) due to severe acute respiratory syndrome (SARS) attributed to COVID-19 among children and adolescents in Brazil from 2020 to 2021. A cluster method was used to group federal units (FUs) based on HMR. In 2020, clusters with high HMRs were formed by north/northeast FUs. In 2021, there was a reduction in HMR. Clusters with higher rates remained in the N/NE region. Regional differences were observed in the HMR. The findings may reflect social inequalities and access to hospital care, especially in the under 1-year-old age group due to the severity of the disease in this group.

Abstract The objective was to perform a spatial analysis of the hospital mortality rate (HMR) due to severe acute respiratory syndrome (SARS) attributed to COVID-19 among children and adolescents in Brazil from 2020 to 2021. A cluster method was used to group federal units (FUs) based on HMR. In 2020, clusters with high HMRs were formed by north/northeast FUs. In 2021, there was a reduction in HMR. Clusters with higher rates remained in the N/NE region. Regional differences were observed in the HMR. The findings may reflect social inequalities and access to hospital care, especially in the under 1-year-old age group due to the severity of the disease in this group.

Resumo Objetivou-se realizar uma análise espacial da taxa de mortalidade hospitalar (TMH) por síndrome respiratória aguda grave (SRAG) atribuída à COVID-19 em crianças e adolescentes no Brasil no período de 2020 a 2021. Utilizou-se o método de cluster para agrupar as unidades federativas (UFs) com base na TMH. Em 2020, clusters com altas TMHs foram formados por UFs Norte/Nordeste. Em 2021, houve redução na TMH. Os clusters com maiores taxas permaneceram na região N/NE. Diferenças regionais foram observadas nas TMHs. Os achados podem refletir as desigualdades sociais e o acesso à atenção hospitalar, principalmente na faixa etária de menores de 1 ano pela gravidade da doença neste grupo.

ABSTRACT We present a case report detailing the successful phacoemulsification surgery with artificial iris implantation for two individuals with oculocutaneous albinism. These women suffered from cataracts, resulting in reduced visual acuity and heightened photophobia due to iris pigmentary epithelium deficiency. The patients underwent phacoemulsification along with prosthetic artificial iris implantation into the posterior chamber. This intervention resulted in improved visual acuity, reduced photophobia and glare, and an overall enhanced quality of life. Our report highlights two cases of successful phacoemulsification and artificial iris implantation in patients with oculocutaneous albinism and cataracts, leading to improved visual acuity, reduced photophobia, and enhanced quality of life. Notably, there are no prior records in South American literature of cataract surgery combined with artificial iris implantation for oculocutaneous albinism patients up to the time of this publication. cataracts deficiency chamber glare life Notably publication

Abstract Concrete presents complex behavior, being a difficult material to define the ideal proportion of its constituents. To overcome this challenge, many mix design methods have been developed over time. However, most of these methods do not consider durability parameters during the procedure. Thus, the objective of this article is to present a mix design method for concrete, with properties of workability (slump rating from 50 mm to 220 mm), axial compressive strength (class of 25MPa to 55MPa), and durability as response parameters. From the application of the proposed method, it was possible to create mix design and performance diagrams. It was noticed that all concretes fell into the pre-established consistency class and presented axial compressive strength results close to the predetermined values. Moreover, it was possible to obtain indications of the material's durability. behavior constituents challenge time However procedure Thus concrete slump 5 22 mm, , mm) MPa 55MPa, 55MPa 55MPa) diagrams preestablished pre established values Moreover materials s 2

Resumo O concreto apresenta um comportamento complexo, sendo uma atividade difícil definir a proporção ideal dos seus constituintes. A fim de superar esse desafio, muitos métodos de dosagem foram desenvolvidos, ao longo do tempo. Entretanto, a maioria desses métodos não considera parâmetros de durabilidade durante o procedimento. Assim, o objetivo deste artigo é apresentar um método para dosar concretos, tendo como parâmetros de resposta propriedades de trabalhabilidade (valores de abatimento de 50 mm a 220 mm), de resistência à compressão axial (classe de 25MPa a 55MPa) e de durabilidade. A partir da aplicação do método proposto, foi possível elaborar os diagramas de dosagem e de desempenho. Percebeu-se que todos os concretos se enquadraram na classe de consistência pré-estabelecida e apresentaram resultados de resistência à compressão axial próximos aos valores pré-determinados. Além disso, foi possível obter indicativos da durabilidade do material. complexo constituintes desafio desenvolvidos tempo Entretanto procedimento Assim 5 22 mm, , mm) MPa 55MPa proposto desempenho Percebeuse Percebeu préestabelecida pré estabelecida prédeterminados. prédeterminados determinados. determinados pré-determinados disso material 2

The industry's principal objective is to comprehend the optimal application of each metal alloy in terms of corrosion resistance. A key focus lies in exploring alternative approaches to address this corrosive process in a manner that is both sustainable and economically feasible. The major strategy to mitigate this contest involves the utilization of inhibitors. However, it is imperative to note that certain inhibitors pose environmental risks. This study explores the effect of the addition of protic ionic liquids (PILs) on the corrosion of carbon steel (A36) in a saline solution (3.5 wt% NaCl), considering variations in pH and temperature. It focuses on understanding the effects of pH and temperature on the PILs' ability to protect the steel surface. Notably, changes in pH do not affect the protective capacity of the inhibitors. Efficiency values exceeding 72% were achieved under both acidic conditions tested. The study reveals two scenarios: At 40 °C, the PILs effectively protect the steel, with an efficiency of approximately 74% at concentrations of 500 and 1000 ppm. However, at 60 °C, efficiency decreases notably, reaching a maximum of 51% at a concentration of 500 ppm. Afterward, to evaluate the protective effectiveness of these compounds, gravimetric and electrochemical impedance spectroscopy (EIS) serve as the main methods, accompanied by optical and atomic force microscopy (AFM) for evaluating the surface. In electrochemical tests, PILs 1 and 2 exhibit superior efficiency, with film formation, while PILs 3 to 6 demonstrate comparatively lower values. industrys industry s resistance feasible However risks (PILs A36 (A36 3.5 35 5 (3. wt NaCl, NaCl , NaCl) surface Notably 72 tested scenarios 4 C °C 74 50 100 ppm notably 51 Afterward compounds EIS (EIS methods AFM (AFM tests formation A3 (A3 3. (3 7 10 (A (

Abstract: The aim of this study was to estimate genetic parameters and select superior mango genotypes using the mixed-models approach. The 16 genotypes, varieties and hybrid selections, were evaluated regarding physical and physical-chemical traits of the fruit using the REML/BLUP methodology. Mango fruit weight and pulp weight can be selected indirectly based on fruit length and diameter, which are more easily evaluated. The hybrids CPAC 26394, Lita, and Rosa 46 stand out in regard to fruit size. The genotypes R12P09, CPAC 2293, Roxa, Omega, Alfa, and Lita have better quality fruit than the Tommy Atkins variety that is widely used in commercial orchards. The Roxa variety has pulp with little or no fiber, a trait that is of great importance to consumers and for industrial processing. The genotypes Alfa, CPAC 5895, Ômega, R10P08, R12P09, R13P10, Rosa 2, Rosa 36, and Rosa 46 have pulp free of internal breakdown. The varieties and hybrid selections of mango show variability regarding fruit traits, and the REML/BLUP methodology is efficient for selection of genotypes with desirable fruit traits in mango. Abstract mixedmodels mixed models approach 1 physicalchemical chemical REMLBLUP REML BLUP diameter 26394 4 size R12P09 RP R P 2293 Omega Alfa orchards fiber processing 5895 Ômega R10P08 R13P10 2 36 breakdown 2639 R12P0 229 589 R10P0 R13P1 3 263 R12P 22 58 R10P R13P 26 5

Resumo: Objetivou-se estimar parâmetros genéticos e selecionar genótipos superiores de mangueira, utilizando a abordagem de modelos mistos. Os 16 genótipos, variedades e seleções híbridas, foram avaliados quanto aos caracteres físicos e físico-químicos do fruto, utilizando a metodologia REML/BLUP. A massa do fruto e a massa da polpa de mangueira podem ser selecionadas indiretamente com base no comprimento e no diâmetro do fruto, que são mais facilmente avaliados. Os híbridos CPAC 26394, Lita e Rosa 46 destacam-se quanto ao tamanho do fruto. Os genótipos R12P09, CPAC 2293, Roxa, Omega, Alfa e Lita apresentam frutos de melhorqualidade do que a variedade Tommy Atkins, bastante utilizada em pomares comerciais. Avariedade Roxa apresenta polpa com pouca ou nenhuma fibra, característica muito requisitadapor consumidores e para processamento industrial. Já os genótipos Alfa, CPAC 5895, Ômega, R10P08, R12P09, R13P10, Rosa 2, Rosa 36 e Rosa 46 apresentam polpa livre de colapso interno. Resumo Objetivouse Objetivou se mistos 1 híbridas físicoquímicos físico químicos REMLBLUP REML BLUP REML/BLUP 26394 4 destacamse destacam R12P09 RP R P 2293 Omega Atkins comerciais fibra industrial 5895 Ômega R10P08 R13P10 2 3 interno 2639 R12P0 229 589 R10P0 R13P1 263 R12P 22 58 R10P R13P 26 5

Abstract The objective was to perform a spatial analysis of the hospital mortality rate (HMR) due to severe acute respiratory syndrome (SARS) attributed to COVID-19 among children and adolescents in Brazil from 2020 to 2021. A cluster method was used to group federal units (FUs) based on HMR. In 2020, clusters with high HMRs were formed by north/northeast FUs. In 2021, there was a reduction in HMR. Clusters with higher rates remained in the N/NE region. Regional differences were observed in the HMR. The findings may reflect social inequalities and access to hospital care, especially in the under 1-year-old age group due to the severity of the disease in this group. HMR (HMR SARS (SARS COVID19 COVID 19 COVID-1 202 2021 FUs (FUs northnortheast north northeast NNE N NE region care 1yearold yearold 1 year old COVID1 COVID- 20 2

Resumo Objetivou-se realizar uma análise espacial da taxa de mortalidade hospitalar (TMH) por síndrome respiratória aguda grave (SRAG) atribuída à COVID-19 em crianças e adolescentes no Brasil no período de 2020 a 2021. Utilizou-se o método de cluster para agrupar as unidades federativas (UFs) com base na TMH. Em 2020, clusters com altas TMHs foram formados por UFs Norte/Nordeste. Em 2021, houve redução na TMH. Os clusters com maiores taxas permaneceram na região N/NE. Diferenças regionais foram observadas nas TMHs. Os achados podem refletir as desigualdades sociais e o acesso à atenção hospitalar, principalmente na faixa etária de menores de 1 ano pela gravidade da doença neste grupo. Objetivouse Objetivou se TMH (TMH SRAG (SRAG COVID19 COVID 19 COVID-1 202 2021 Utilizouse Utilizou (UFs NorteNordeste Norte Nordeste Norte/Nordeste NNE N NE N/NE grupo COVID1 COVID- 20 2

Abstract Vegetative propagation methods of acerola cherry are important in standardizing orchards and fruit quality, and the cutting process has been investigated as a promising alternative. The present work aimed to propose the best combination of substrate and cutting type (herbaceous and semi-woody) to provide a greater root rate and seedling development. The trial was conducted in a greenhouse at the State University of Maranhão, in São Luís - MA under intermittent nebulization conditions. The cuttings with dimensions of 10 cm were treated with indole butyric acid (IBA) at a concentration of 2,000 mg L-1, and as substrate were used vermiculite (MV), Plantmax ® (PL) (commercial substrate based on decomposed pine bark), agricultural soil + tanned and sieved bovine manure (S + M) (50% + 50%, v / v); and vegetable soil (VS) composed of fresh soil with the remains of decomposed plants (leaves, stems, bark, and tree fern). The experiment was laid out in a randomized complete design in a 2 x 4 factorial arrangement (2 types of cuttings x 4 substrates) with eight replications with five cuttings per plot. At 60 days after the establishment of the trial, it was concluded that the best combination for rooting cuttings was vermiculite with herbaceous cuttings because they favor a higher rooting rate (95.0%). The alternative substrate composed of soil and tanned bovine manure provided promising results in the rate of rooting (72.5%), root formation, and vegetative development of the aerial part. quality semiwoody semi woody semi-woody Maranhão conditions 1 IBA (IBA 2000 000 2,00 L1, L1 L 1, L-1 MV, MV , (MV) PL (PL commercial bark bark) S M 50% 50 (50 v) VS (VS leaves, leaves (leaves stems fern. fern . fern) ( substrates plot 6 95.0%. 950 95.0% 95 0 (95.0%) 72.5%, 725 72.5% 72 5 (72.5%) formation part 200 00 2,0 L- (MV (5 95.0 9 (95.0% 72.5 7 (72.5% 20 2, 95. (95.0 72. (72.5 (95. (72. (95 (72 (9 (7

Abstract The objective was to perform a spatial analysis of the hospital mortality rate (HMR) due to severe acute respiratory syndrome (SARS) attributed to COVID-19 among children and adolescents in Brazil from 2020 to 2021. A cluster method was used to group federal units (FUs) based on HMR. In 2020, clusters with high HMRs were formed by north/northeast FUs. In 2021, there was a reduction in HMR. Clusters with higher rates remained in the N/NE region. Regional differences were observed in the HMR. The findings may reflect social inequalities and access to hospital care, especially in the under 1-year-old age group due to the severity of the disease in this group.

Resumo Objetivou-se realizar uma análise espacial da taxa de mortalidade hospitalar (TMH) por síndrome respiratória aguda grave (SRAG) atribuída à COVID-19 em crianças e adolescentes no Brasil no período de 2020 a 2021. Utilizou-se o método de cluster para agrupar as unidades federativas (UFs) com base na TMH. Em 2020, clusters com altas TMHs foram formados por UFs Norte/Nordeste. Em 2021, houve redução na TMH. Os clusters com maiores taxas permaneceram na região N/NE. Diferenças regionais foram observadas nas TMHs. Os achados podem refletir as desigualdades sociais e o acesso à atenção hospitalar, principalmente na faixa etária de menores de 1 ano pela gravidade da doença neste grupo.

RESUMO A síndrome corticobasal (SCB) é uma causa rara de demência e compreende combinações variadas de sinais subcorticais (parkinsonismo acinético-rígido, distonia ou mioclonias) com sinais corticais (apraxia, mão alienígena ou déficit sensorial cortical), geralmente assimétricos. Nosso objetivo foi relatar e comparar as apresentações clínica e de neuroimagem de dois pacientes com diagnóstico de SCB. Enquanto o caso 1 apresentava afasia grave não fluente associada a apraxia leve e rigidez de membros, o caso 2 exibia comprometimento cognitivo mais posterior, com padrão de linguagem distinto, erros visuoespaciais e heminegligência. O FDG PET teve papel significativo no diagnóstico, sugerindo, no primeiro caso, degeneração corticobasal e, no segundo, padrão Alzheimer. A SCB tem sido amplamente estudada com o advento de novos métodos in vivo, como o FDG PET cerebral. Estudos que aprofundem a heterogeneidade fenotípica e de biomarcadores da SCB serão de grande importância para melhor classificação, prognóstico e tratamento da doença. (SCB parkinsonismo acinéticorígido, acinéticorígido acinético rígido, rígido acinético-rígido mioclonias apraxia, (apraxia cortical, cortical , cortical) assimétricos membros posterior distinto heminegligência sugerindo segundo Alzheimer vivo cerebral classificação doença

ABSTRACT Corticobasal syndrome (CBS) is a rare cause of dementia and comprises varied combinations of subcortical signs (akinetic-rigid parkinsonism, dystonia, or myoclonus) with cortical signs (apraxia, alien hand or cortical sensory deficit), usually asymmetric. We aimed to report and compare the clinical and neuroimaging presentation of two patients diagnosed with CBS. While case 1 had severe non-fluent aphasia associated with mild apraxia and limb rigidity, case 2 had a more posterior cognitive impairment, with a different language pattern associated with marked visuospatial errors and hemineglect. FDG PET played a significant role in diagnosis, suggesting, in the first case, corticobasal degeneration and, in the second, Alzheimer's disease pattern. CBS has been widely studied with the advent of new in vivo methods such as brain FDG PET. Studies that deepen the phenotypic and biomarker heterogeneity of CBS will be of great importance for better classification, prognosis, and treatment of the condition. (CBS akineticrigid akinetic rigid parkinsonism dystonia myoclonus apraxia, (apraxia deficit, deficit , deficit) asymmetric nonfluent non fluent rigidity impairment hemineglect diagnosis suggesting second Alzheimers Alzheimer s classification prognosis condition

Abstract In low and middle-income countries such as Brazil, most maternal deaths are related to hypertensive complications. Preeclampsia is the leading cause of maternal mortality and morbidity. Significant proportion is associated with the following factors: lack of identification of high-risk women, lack of adequate prevention, difficulty in maintaining a high-risk prenatal follow-up, delayed diagnosis, insecurity and low use of magnesium sulphate, delayed pregnancy interruption and lack of postpartum follow-up of these high-risk cases. Four major actions are proposed to minimize this alarming clinical picture and reduce the mortality rates due to preeclampsia, called the "4 P Rule" (Adequate Prevention – Vigilant Prenatal Care – Timely Delivery (Parturition) – Safe Postpartum). From this simple "rule" we can open a range of important processes and reminders that may help in the guidance of preeclampsia management. middleincome middle income Brazil complications morbidity factors highrisk high risk women prevention followup, followup follow up, up diagnosis sulphate cases 4 " Rule Adequate Parturition (Parturition Postpartum. Postpartum . Postpartum) rule "rule management

Abstract In reproductive technologies, uncovering the molecular aspects of oocyte and embryo competence under different conditions is crucial for refining protocols and enhancing efficiency. RNA-seq generates high-throughput data and provides transcriptomes that can undergo additional computational analyses. This study presented the transcriptomic profiles of in vitro matured oocytes and blastocysts produced in vitro from buffalo crossbred (Bubalus bubalis), coupled with gene co-expression and module preservation analysis. Cumulus Oophorus Complexes, obtained from slaughterhouse-derived ovaries, were subjected to in vitro maturation to yield metaphase II oocytes (616) or followed in vitro fertilization and culture to yield blastocysts for sequencing (526). Oocyte maturation (72%, ±3.34 sd) and embryo development (21.3%, ±4.18 sd) rates were obtained from three in vitro embryo production routines following standard protocols. Sequencing of 410 metaphase II oocytes and 70 hatched blastocysts (grade 1 and 2) identified a total of 13,976 genes, with 62% being ubiquitously expressed (8,649). Among them, the differentially expressed genes (4,153) and the strongly variable genes with the higher expression (fold-change above 11) were highlighted in oocytes (BMP15, UCHL1, WEE1, NLRPs, KPNA7, ZP2, and ZP4) and blastocysts (APOA1, KRT18, ANXA2, S100A14, SLC34A2, PRSS8 and ANXA2) as representative indicators of molecular quality. Additionally, genes exclusively found in oocytes (224) and blastocysts (2,200) with specific biological functions were identified. Gene co-expression network and module preservation analysis revealed strong preservation of functional modules related to exosome components, steroid metabolism, cell proliferation, and morphogenesis. However, cell cycle and amino acid transport modules exhibited weak preservation, which may reflect differences in embryo development kinetics and the activation of cell signaling pathways between buffalo and bovine. This comprehensive transcriptomic profile serves as a valuable resource for assessing the molecular quality of buffalo oocytes and embryos in future in vitro embryo production assays. technologies efficiency RNAseq RNA seq highthroughput high throughput analyses Bubalus bubalis, bubalis , bubalis) coexpression co Complexes slaughterhousederived slaughterhouse derived ovaries 616 (616 526. 526 . (526) 72%, 72 (72% 334 3 34 ±3.3 sd 21.3%, 213 21 (21.3% 418 4 18 ±4.1 41 7 grade 2 13976 13 976 13,97 62 8,649. 8649 8,649 8 649 (8,649) them 4,153 4153 153 (4,153 foldchange fold change 11 BMP15, BMP15 BMP (BMP15 UCHL1 UCHL WEE1 WEE NLRPs KPNA7 KPNA ZP2 ZP ZP4 APOA1, APOA1 APOA (APOA1 KRT18 KRT ANXA2 ANXA S100A14 SA S A SLC34A2 SLCA SLC PRSS Additionally 224 (224 2,200 2200 200 (2,200 components metabolism proliferation morphogenesis However bovine assays 61 (61 52 (526 72% (72 33 ±3. 21.3% (21.3 ±4. 1397 97 13,9 6 864 8,64 64 (8,649 4,15 415 15 (4,15 BMP1 (BMP1 (APOA KRT1 S100A1 SLC34A 22 (22 2,20 220 20 (2,20 (6 5 (52 (7 ±3 21.3 (21. ±4 139 9 13, 86 8,6 (8,64 4,1 (4,1 (BMP S100A (2 2,2 (2,2 ( (5 ± 21. (21 8, (8,6 4, (4, 2, (2, (8, (4 (8

Abstract Food supplements commonly present nutritional quality, especially in plant-origin foods, contributing to the population's quality of life. The use of babassu mesocarp flour in the preparation of food supplements contributes to human nutrition as it is a nutritionally balanced food concerning carbohydrates, proteins, lipids, and minerals. Babassu fruits were collected in five municipalities in the state of Maranhão/Brazil, and the mesocarp was extracted to develop four food supplement formulations per municipality. Firstly, babassu mesocarp flour was prepared in a drying and sterilization process and, subsequently, the manufacturing process of the babassu coconut flour food supplement was added with natural soy extract. The samples were subjected to proximate composition analysis to determine the percentages of carbohydrates, lipids, proteins, moisture, lignin, and energy values (kcal/100 g). The study presented a product rich in carbohydrates (86%), protein values above 10% in the composition, and an average of 19% lipids, presenting a nutritional balance suitable for daily use by the population and even high-performance athletes. plantorigin plant origin foods populations s life proteins lipids minerals MaranhãoBrazil Maranhão Brazil Maranhão/Brazil municipality Firstly subsequently extract moisture lignin kcal/100 kcal100 kcal 100 (kcal/10 g. g . g) 86%, 86 86% , (86%) 10 19 highperformance high performance athletes kcal/10 kcal10 (kcal/1 8 (86% 1 kcal/1 kcal1 (kcal/ (86 kcal/ (kcal (8 (