Abstract Background: Real-world, primary data on the treatment of psoriasis are scarce, especially concerning the role of soluble biomarkers as outcome predictors. Objective: The authors evaluated the utility of Th1/Th17 serum cytokines along with clinical characteristics as predictors of drug survival in the treatment of psoriasis. Methods: The authors consecutively included participants with moderate to severe psoriasis who were followed up for 6 years. Baseline interferon-α, tumor necrosis factor-α, and inter-leukin (IL)-2, IL-4, IL-6, IL-10, and IL-17A were measured using a cytometric bead array; clinical data were assessed. The authors calculated hazard ratios (HRs) for drug survival using a Cox proportional hazards model. Results: The authors included 262 patients, most of whom used systemic immunosuppressants or biologics. In the multivariate model, poor quality of life measured by the Dermatology Life Quality Index (HR = 1.04; 95% CI 1.01–1.07; p = 0.012) and elevated baseline IL-6 (HR = 1.99; 95% CI 1.29–3.08; p = 0.002) were associated with treatment interruption. Study limitations: The main limitation of any cohort study is the presence of confounders that could not be detected in clinical evaluation. Conclusions: Poor quality of life and elevated baseline serum IL-6 level predicted treatment interruption in patients with moderate to severe psoriasis. Although IL-6 is not the most important mediator of the inflammatory pathway in the skin environment, it is an interesting biomarker candidate for predicting psoriasis treatment response. Background Realworld, Realworld Real world, world Real-world scarce Objective Th1Th17 ThTh Th1 Th17 Th Th1/Th1 Methods years interferonα, interferonα interferon α, α interferon-α factorα, factorα factor factor-α interleukin inter leukin IL2, IL2 IL 2, 2 (IL)-2 IL4, IL4 4, 4 IL-4 IL6, IL6 6, IL10, IL10 10, 10 IL-10 IL17A ILA 17A A array assessed HRs (HRs model Results 26 biologics HR 1.04 104 1 04 95 1.01–1.07 101107 01 07 0.012 0012 0 012 IL- 1.99 199 99 1.29–3.08 129308 29 3 08 0.002 0002 002 limitations evaluation Conclusions environment response Th1Th1 Th1/Th (IL)- IL1 IL-1 1.0 9 1.01–1.0 10110 0.01 001 1.9 19 1.29–3.0 12930 0.00 000 00 Th1Th (IL) 1. 1.01–1. 1011 0.0 1.29–3. 1293 (IL 1.01–1 101 0. 1.29–3 129 1.01– 1.29– 12 1.01 1.29 1.2

ABSTRACT BACKGROUND: Specific types of violence such as intimate partner sexual violence and intimate partner homicide occur more frequently in rural areas. OBJECTIVE: This study aimed to systematically review the literature on the knowledge and attitudes of rural healthcare providers regarding cases of domestic violence against women. DESIGN AND SETTING: Systematic review developed at Universidade Federal de Uberlândia. METHODS: We conducted an electronic search of six databases, which only included observational studies, regardless of the year, language, or country of publication, except for studies that used secondary data and were exclusively qualitative. Two reviewers performed the selection, data extraction, and risk of bias assessment using a specific Joanna Briggs Institute tool. RESULTS: Six studies met the inclusion criteria. All the studies had a low risk of bias. Approximately 38% of these professionals identified injuries caused by violence in patients. When asked about knowing the correct attitude to take in cases of confirmed violence, between 12% and 64% of rural healthcare providers answered positively; most of them would refer to specialized institutions and promote victim empowerment and counseling. The number of professionals with an educational background in the field ranged from 16% to 98%. CONCLUSIONS: The evident disparity across studies shows that some professionals have suboptimal knowledge and require training to adopt the correct attitude when identifying female victims of domestic violence in clinical practice. SYSTEMATIC REVIEW REGISTRATION: This systematic review was registered in the Open Science Framework Database under the registration http://doi.org/10.17605/OSF.IO/B7Q6S. BACKGROUND areas OBJECTIVE women SETTING Uberlândia METHODS databases year language publication qualitative selection extraction tool RESULTS criteria 38 patients 12 64 positively counseling 16 98 98% CONCLUSIONS practice REGISTRATION http//doi.org/10.17605/OSF.IO/B7Q6S. httpdoiorg1017605OSFIOB7Q6S httpdoiorgOSFIOBQS http //doi.org/10.17605/OSF.IO/B7Q6S. doi org 10 17605 OSF IO B7Q6S B Q S http://doi.org/10.17605/OSF.IO/B7Q6S 3 1 6 9 http//doi.org/10.17605/OSF.IO/B7Q6S httpdoiorg OSFIOB doiorg1017605OSFIOB7Q6S doiorgOSFIOBQS //doi.org/10.17605/OSF.IO/B7Q6S 1760 BQS doiorg 176 17

Resumen: El objetivo fue describir las acciones de vigilancia y seguridad de la vacuna contra mpox (Jynneos) en Brasil, del 2022 al 2023. Se realizó un estudio descriptivo de los casos de mpox y de los eventos supuestamente atribuibles a la vacunación o inmunización para la vacuna Jynneos, registrados en los sistemas de información. Se calcularon frecuencias absolutas y relativas y coeficientes de notificación de eventos supuestamente atribuibles a la vacunación o inmunización por 1.000 dosis administradas. Se registraron 9.596 casos de mpox y se distribuyeron 49.000 dosis de vacuna, de estas se administraron 14.395 (31%) dosis y se reportaron 65 eventos supuestamente atribuibles a la vacunación o inmunización (4,5 por 1.000 dosis administradas). Todos los eventos supuestamente atribuibles a la vacunación o inmunización no fueron graves y 22 (33,8%) casos tuvieron reacciones relacionadas con el producto. Los resultados son acordes a la frecuencia esperada. Sin embargo, puede que el número de dosis administradas no haya sido suficiente para detectar eventos raros o inusuales. Por lo tanto, es necesaria una vigilancia continua para garantizar la eficacia y la seguridad de la vacunación, especialmente entre los grupos con mayor riesgo de contraer la enfermedad.

Abstract: This study describes the surveillance and safety actions of the mpox (Jynneos) vaccine in Brazil, from 2022 to 2023. A descriptive study of mpox cases and events supposedly attributable to vaccination and/or immunization for Jynneos vaccine, recorded in the information systems, was conducted. Absolute and relative frequencies and reporting coefficients of events supposedly attributable to vaccination and/or immunization per 1,000 administered doses were calculated. A total of 9,596 cases of mpox were registered and 49,000 doses of vaccine were distributed, of which 14,395 (31%) were administered and 65 events supposedly attributable to vaccination and/or immunization (4.5 per 1,000 administered doses) were notified. All events supposedly attributable to vaccination and/or immunization were non-serious, and 22 (33.8%) cases had product-related reactions. Results are within the expected frequency; however, the number of administered doses may not have been sufficient to detect rare or unusual events. Continued surveillance is necessary to ensure vaccination effectiveness and safety, especially among groups at higher risk for the disease.

Resumo: O objetivo deste estudo é descrever as ações de vigilância e segurança da vacina contra a mpox (Jynneos) no Brasil, de 2022 a 2023. Realizou-se estudo descritivo dos casos de mpox e dos eventos supostamente atribuíveis à vacinação e/ou imunização para vacina Jynneos, registrados nos sistemas de informação. Foram calculadas frequências absolutas e relativas e coeficientes de notificação de eventos supostamente atribuíveis à vacinação e/ou imunização por mil doses administradas. Foram registrados 9.596 casos de mpox e distribuídas 49 mil doses de vacina; dessas, 14.395 (31%) doses foram administradas e 65 eventos supostamente atribuíveis à vacinação e/ou imunização (4,5 por mil doses administradas) foram notificados. Todos os eventos supostamente atribuíveis à vacinação e/ou imunização foram não graves e 22 (33,8%) casos apresentaram reações relacionadas ao produto. Os resultados estão dentro da frequência esperada. No entanto, o número de doses administradas pode não ter sido suficiente para detectar eventos raros ou inusitados. Portanto, a vigilância contínua é necessária para garantir a efetividade e a segurança da vacinação, especialmente entre os grupos de maior risco.

RESUMO Objetivo: Avaliar a acurácia de médicos generalistas em reconhecer a retinopatia diabética por meio da retinografia colorida, com um curso de capacitação com duração de 2 horas, comparando a capacidade de rastrear e classificar a retinopatia diabética em relação ao exame presencial com oftalmologista. Métodos: No primeiro braço do estudo, de 142 pacientes diabéticos incluídos, avaliaram-se 274 olhos, em que esses pacientes foram examinados com oftalmoscópio binocular indireto e classificados quanto ao grau da retinopatia diabética. No segundo braço do estudo, 14 médicos não especialistas em oftalmologia receberam um treinamento de 2 horas para o diagnóstico de retinopatia diabética com a retinografia colorida e se aferiu a acurácia desses profissionais em rastrear a retinopatia diabética antes e depois do curso de capacitação, utilizando as retinografias obtidas na primeira frente do estudo. Resultados: Verificou-se aumento significativo da sensibilidade (82% para 99%) e da especificidade (44% para 83%) na detecção da retinopatia diabética pelos médicos generalistas, com o curso de capacitação. Conclusão: O médico generalista capacitado pode avaliar a retinopatia diabética por meio da retinografia colorida, sendo o programa de rastreamento dessa complicação do diabetes uma proposta viável e benéfica ao país. Objetivo oftalmologista Métodos estudo incluídos avaliaramse avaliaram 27 olhos 1 Resultados Verificouse Verificou 82% 82 (82 99% 99 44% 44 (44 83% 83 Conclusão país 8 (8 9 4 (4 (

ABSTRACT Objective: To assess the accuracy of general practitioners in recognizing diabetic retinopathy through color retinal retinography, with a two-hour training course, by comparing the capacity of screening and classifying diabetic retinopathy. Methods: In the first arm of the study, of 142 diabetic patients included, 274 eyes were evaluated, and these patients were examined with an indirect binocular ophthalmoscope and classified according to the degree of diabetic retinopathy. In the second arm of the study, 14 physicians who are not specialists in ophthalmology received two-hour training to diagnose diabetic retinopathy with color retinography, and the accuracy of these professionals in screening for diabetic retinopathy before and after the training course was measured using the photographic images obtained on the first front of the study. Results: There was a significant increase in sensitivity (82% to 99%) and specificity (44% to 83%) in detecting diabetic retinopathy by general practitioners, after attending the training. Conclusion: Qualified general practitioners can assess diabetic retinopathy through color retinography, and the screening program for this diabetes complication is a viable and beneficial proposal for the country. Objective retinography twohour two hour Methods study included 27 evaluated 1 Results 82% 82 (82 99% 99 44% 44 (44 83% 83 Conclusion country 2 8 (8 9 4 (4 (

Silver(I) complexes of amantadine (atdH), memantine (mtnH) and rimantadine (rtdH), named Ag-atd, Ag-mtn and Ag-rtd, respectively, were recently synthesized and described in the literature as promising antibacterial agents. In the present study, the cytotoxicity of such complexes was evaluated against cultures of primary epidermal keratinocytes (HaCaT) and murine melanoma tumor cells (B16-F10), and mutagenicity was studied by the Ames test to investigate their abilities to induce gene mutations. The Ames test was performed using Salmonella Typhimurium strains (TA98, TA100, TA102 and TA97a) capable of detecting frameshift and base pair substitution gene mutations, in experiments with and without metabolic activation (microsomal fraction S9). This study revealed significant cytotoxic activity against tumor cells and selectivity of Ag-atd and Ag-rtd complexes when compared to non-tumor human keratinocyte cells. Moreover, the Ag(I) complexes did not induce a significant growth in the number of revertant colonies when comparing with the negative control, both in the experiments without and with metabolic activation, indicating absence of mutagenic activity. The results are encouraging and collaborate in the genotoxicological investigations necessary for understanding the interaction and ability of the silver complexes to induce mutations and contribute to ensure their uses as future antibacterial or antitumor agents. SilverI Silver I Silver(I atdH, atdH , (atdH) mtnH (mtnH rtdH, rtdH (rtdH) Agatd, Agatd Ag atd, atd Agmtn mtn Agrtd, Agrtd rtd, rtd respectively agents HaCaT (HaCaT B16F10, B16F10 BF B16 F10 B F (B16-F10) TA98, TA98 TA (TA98 TA100 TA10 TA97a TAa microsomal S9. S9 S . S9) nontumor non Moreover AgI Ag(I control (atdH (rtdH B16F1 B1 F1 (B16-F10 TA9 (TA9 TA1 B16F (B16-F1 (TA (B16-F

Resumo: O artigo aborda as relações entre subjetividade e trabalho escravo contemporâneo a partir da narrativa de trabalhadores(as) escravazados(as). Foi realizada uma pesquisa social qualitativa em uma perspectiva construcionista. Buscamos a interação com trabalhadores resgatados e realizamos a observação participante da rotina de trabalhadores atendidos em um projeto institucional, com diário de campo, e entrevistas individuais (semiestruturadas e abertas) com trabalhadores(as) e equipe do projeto institucional. O trabalho de campo durou um ano e meio, e a pesquisa foi realizada com o suporte da Análise de Conteúdo. Para a interpretação utilizamos aportes da teoria freudiana e do pensamento de Foucault, com os quais foi possível compreender aspectos das vivências dos trabalhadores, características da exploração, abandonos parentais e tensões em torno da autoclassificação como “escravo”. As narrativas apontaram uma realidade dramática manifesta na exploração do corpo, em abusos autoritários, na violência e negligência. Ao mesmo tempo, as narrativas evidenciaram formas de resistência dos trabalhadores que convocam mais investigações para adensar o conhecimento sobre as experiências subjetivas desses que estão num lugar de escravizado(a). Resumo trabalhadoresas trabalhadores(as escravazadosas. escravazadosas escravazados . escravazados(as) construcionista institucional semiestruturadas abertas meio Conteúdo Foucault escravo. “escravo” corpo autoritários negligência tempo escravizadoa. escravizadoa escravizado escravizado(a) escravazados(as “escravo escravizado(a

Abstract: This study addresses the relation between subjectivity and contemporary enslaved labor from the enslaved workers’ narratives in Brazil. A qualitative social research was carried out based on a constructionist perspective. We sought interaction with rescued workers and used (a) participant observation of workers’ routine in an institutional project that supports them and a field diary, (b) semi-structured and open individual interviews with workers and a member of the team project. The fieldwork lasted a year and a half and the analysis followed Content Analysis. Freudian theory and Foucault’s thought were used for interpretation, which managed to understand aspects of workers’ experiences, exploitation characteristics, parental abandonment, as well as the tensions in self-classification as enslaved. The narratives pointed to a dramatic reality manifested in body exploitation, authoritarian abuses, violence, and negligence. At the same time, these narratives showed forms of worker resistance that calls for further investigations to increase knowledge on the subjective experiences of those who were enslaved. Abstract Brazil perspective (a diary b (b semistructured semi structured Analysis Foucaults Foucault s interpretation characteristics abandonment selfclassification self classification abuses violence negligence time

Resumen: Este artículo aborda la relación entre la subjetividad y el trabajo esclavo contemporáneo desde la narrativa de trabajadores esclavizados. Se realizó una investigación social cualitativa desde una perspectiva construccionista. Buscamos la interacción con los trabajadores liberados y utilizamos la observación participante de la rutina de los trabajadores atendidos en un proyecto institucional, diario de campo y entrevistas individuales (semiestructuradas y abiertas) con trabajadores y miembros del equipo del proyecto institucional. El trabajo de campo duró un año y medio, y se utilizó como apoyo el análisis de contenido. Para la interpretación se utilizaron aportes de la teoría freudiana y el pensamiento de Foucault, con lo que fue posible comprender aspectos de las vivencias de los trabajadores, características del escenario de explotación, abandono parental y las tensiones relacionadas con la autoclasificación “esclavo”. Las narrativas apuntan a una realidad dramática manifestada en la explotación del cuerpo, abuso autoritario, violencia y abandono. Al mismo tiempo, evidenciaron formas de resistencia por parte de los trabajadores, que reclaman más investigaciones para profundizar en el conocimiento sobre las vivencias subjetivas de quienes se encuentran en esclavitud. Resumen esclavizados construccionista institucional semiestructuradas abiertas medio contenido Foucault esclavo. . “esclavo” cuerpo autoritario tiempo esclavitud “esclavo

Abstract This study assessed the features of the 100 most-cited papers on diabetes mellitus (DM) in dentistry using bibliometric measures. A search of the most cited papers on DM using journals included in the category “Dentistry, Oral Surgery and Medicine” in the Web of Science database up to January 2023 was performed. The complete bibliographic records of the selected papers were exported in plain text or Research Information Systems (RIS) file format. The following bibliometric indicators were collected: title, year, authors, number of citations, mean number of citations, institution, country, continent, study design, journal, impact factor, and keywords. Graphical bibliometric networks were created using the VOSviewer software. The number of citations for the 100 most-cited papers in DM research ranged from 111 to 566. Six papers each had more than 400 citations. Most were observational studies (n = 50) from the United States (USA) (n = 23) and were published in the Journal of Periodontology (30%; n=30). Robert Genco was the most cited author and contributed the most to the top 100 articles (3,653 citations; n = 13). The VOSviewer map of co-authorship showed the existence of clusters in research collaboration. The most prolific institutions were the Universities of Buffalo and Michigan (n = 6 each). “Diabetes mellitus” was the most frequent keyword, with 31 occurrences. In conclusion, the most cited studies that investigated the relationship between dentistry and DM were in periodontology. Observational studies, primarily from the USA, have been the most cited thus far. 10 mostcited (DM measures Dentistry, Dentistry “Dentistry Medicine 202 performed RIS (RIS format collected title year authors institution country continent design journal factor keywords software 11 566 40 50 USA (USA 23 30% 30 (30% n=30. n30 n=30 . n=30) 3,653 3653 3 653 (3,65 13. 13 13) coauthorship co authorship collaboration each. each) Diabetes keyword occurrences conclusion periodontology far 1 20 56 4 5 2 (30 n3 n=3 3,65 365 65 (3,6 (3 n= 3,6 36 (3, ( 3,

BACKGROUND Giant viruses have brought new insights into different aspects of virus-cell interactions. The resulting cytopathic effects from these interactions are one of the main aspects of infection assessment in a laboratory routine, mainly reflecting on the morphological features of an infected cell. OBJECTIVES In this work, we follow the entire kinetics of the cytopathic effect in cells infected by viruses of the Mimiviridae family, spatiotemporally quantifying typical features such as cell roundness, loss of motility, decrease in cell area and cell lysis. METHODS Infections by Acanthamoeba polyphaga mimivirus (APMV), Tupanvirus (TPV) and M4 were carried out at multiplicity of infection (MOI) 1 and MOI 10 in Acanthamoeba castellanii. Monitoring of infections was carried out using time lapse microscopy for up to 72 hours. The images were analyzed using ImageJ software. FINDINGS The data obtained indicate that APMV is the slowest virus in inducing the cytopathic effects of rounding, decrease in cell area, mobility and cell lysis. However, it is the only virus whose MOI increase accelerates the lysis process of infected cells. In turn, TPV and M4 rapidly induce morphological and behavioral changes. MAIN CONCLUSIONS Our results indicate that mimiviruses induce different temporal responses within the host cell and that it is possible to use these kinetic data to facilitate the understanding of infection by these viruses. viruscell routine work family roundness motility APMV, , (APMV) (TPV M (MOI castellanii 7 hours software rounding However turn changes (APMV

RESUMO Objetivos: Analisar as notificações de morte encefálica (ME) recebidas pela Central de Transplantes (CET) do estado de Roraima (RR) de 2017 a 2021. Métodos: Trata-se de estudo transversal, quantitativo e observacional no qual foram analisadas todas as notificações recebidas pela CET no período de 2017 a 2021, resultando em 120 pacientes, dos seguintes centros de saúde: Hospital Geral de Roraima (HGR), Hospital Confederação Nacional das Cooperativas Médicas (UNIMED), Hospital da Criança Santo Antônio (HCSA), Hospital das Clínicas (HC) e Hospital Lotty Íris (HLI). Os dados foram coletados do sistema de gerenciamento de dados da central e repassados para a ficha de coleta desenvolvida pela pesquisadora. Resultados: Este estudo analisou 119 notificações após a exclusão de uma devido a dados conflitantes. Houve predominância do sexo masculino (58%), com trauma cranioencefálico sendo a causa mais frequente de ME. Ao todo, foram realizadas seis captações de órgãos, todas no HGR. Este estudo não encontrou associações significativas entre a captação de órgãos e sexo, idade, local de internação e ano. Conclusão: Há grandes desafios em relação à doação de órgãos em RR, com uma taxa de captação baixa ao longo de 5 anos. Apenas o HGR obteve sucesso em realizar captações de órgãos, diferentemente de outros hospitais notificadores. São necessárias melhorias, destacando a importância de se realizarem mais estudos para uma análise mais aprofundada dos dados. Objetivos ME (ME (CET RR (RR 201 2021 Métodos Tratase Trata transversal 12 pacientes saúde HGR, , (HGR) UNIMED, UNIMED (UNIMED) HCSA, HCSA (HCSA) HC (HC HLI. HLI . (HLI) pesquisadora Resultados 11 conflitantes 58%, 58 58% (58%) todo idade ano Conclusão anos notificadores melhorias 20 202 1 (HGR (UNIMED (HCSA (HLI (58% 2 (58 (5 (

ABSTRACT Objectives: To analyze brain death (BD) notifications received by Central de Transplantes (CET) in the state of Roraima (RR) from 2017 to 2021. Methods: This is a cross-sectional, quantitative, and observational study, where all notifications received by CET from 2017 to 2021 were analyzed, resulting in 120 patients from health the following centers: Hospital Geral de Roraima (HGR), Hospital Confederação Nacional das Cooperativas Médicas (UNIMED), Hospital da Criança Santo Antônio (HCSA), Hospital das Clínicas (HC), and Hospital Lotty Íris (HLI). The data were collected from the center’s data management system and passed on to the collection form developed by the researcher. Results: This study analyzed 119 notifications after excluding one due to conflicting data. There was a predominance of males (58%), with traumatic brain injury being the most frequent cause of BD. Six organ harvestings were carried out, all at the HGR. This study found no significant associations between organ procurement and sex, age, place of hospitalization, and year. Conclusion: There are considerable challenges concerning organ donation in RR, with a low uptake rate over five years. Only the HGR successfully carried out organ harvesting, while other reporting hospitals were unsuccessful. Improvements are needed, highlighting the importance of more studies for a more in-depth data analysis. Objectives BD (BD (CET RR (RR 201 Methods crosssectional, crosssectional cross sectional, sectional cross-sectional quantitative 202 12 centers HGR, , (HGR) UNIMED, UNIMED (UNIMED) HCSA, HCSA (HCSA) HC, HC (HC) HLI. HLI . (HLI) center s researcher Results 11 58%, 58 58% (58%) sex age hospitalization year Conclusion years harvesting unsuccessful needed indepth depth analysis 20 1 (HGR (UNIMED (HCSA (HC (HLI 5 (58% 2 (58 (5 (

RESUMO Introdução: A tonometria gástrica é uma ferramenta útil para examinar a perfusão esplâncnica regional por permitir o fornecimento de uma avaliação indireta do estado do enxerto hepático. Isso ocorre devido à hipoperfusão esplâncnica ser um parâmetro crítico no contexto do transplante de fígado, estando associada ao desenvolvimento de problemas como insuficiência hepática aguda e falência de múltiplos órgãos. Objetivo: Analisar os efeitos da tonometria gastrointestinal no perioperatório dos pacientes submetidos ao transplante hepático. Metodologia: Trata-se de uma Revisão Integrativa realizada nas bases de dados PubMed e BVS. Foram utilizados os descritores: “Tonometry”, “Splanchnic circulation” e “Liver transplantation”, incluindo o operador booleano “AND”, e selecionados artigos de relevância para o tema. Foram selecionados inicialmente 24 artigos, todos publicados nos últimos 20 anos, em português e/ou inglês. Após análise, seis artigos corresponderam ao objetivo proposto. Resultado: Observou-se que a diferença entre a PraCO2 no final da cirurgia e na fase anepática foi maior em pacientes sem disfunção do enxerto hepático. Foi identificada uma correlação positiva entre ΔpraCO2 e o pico de ALT após o transplante de fígado. Em outro estudo, verificou-se que a presença de enzimas hepáticas elevadas e a piora da função hepática sintética, coagulopatia e encefalopatia estava relacionada à má função do enxerto. Também foi comprovado que o pH gástrico intramucoso pode predizer a funcionalidade precoce do enxerto. Em um grupo com disfunção hepática, os pacientes apresentaram pH gástrico intramucoso abaixo de 7,3 no período perioperatório, mantendo-se baixo até a 24ª hora pós-operatória, enquanto o grupo sem disfunção apresentou pH gástrico intramucoso acima de 7,3, exceto na fase anepática, quando ficou abaixo desse valor. Conclusão: Descreve-se a utilidade da tonometria gastrointestinal para monitorar a circulação esplâncnica e a função do enxerto hepático durante o transplante hepático. Embora alguns estudos ofereçam suporte a essa afirmação, esta revisão apresenta limitações devido à quantidade restrita de artigos disponíveis, o que a impede de abranger uma ampla gama de evidências científicas. Introdução fígado órgãos Objetivo Metodologia Tratase Trata se BVS descritores Tonometry, Tonometry , “Tonometry” Splanchnic circulation Liver transplantation, transplantation transplantation” AND, AND “AND” tema 2 anos eou ou inglês análise proposto Resultado Observouse Observou PraCO ΔpraCO estudo verificouse verificou sintética 73 7 3 7, mantendose mantendo ª pósoperatória, pósoperatória pós operatória, operatória pós-operatória valor Conclusão Descrevese Descreve afirmação disponíveis científicas “Tonometry “AND

ABSTRACT Introduction: Gastric tonometry is a useful tool for examining regional splanchnic perfusion as it provides an indirect assessment of the state of the liver graft. This is because splanchnic hypoperfusion is a critical parameter in the context of liver transplantation, being associated with the development of problems such as acute liver failure and multiple organ failure. Objective: To analyze the effects of gastrointestinal tonometry in the perioperative period of patients undergoing liver transplantation. Methodology This is an Integrative Review using the PubMed and VHL databases. The following descriptors were used: “Tonometry”, “Splanchnic circulation” and “Liver transplantation” with the Boolean operator “AND”, and articles of relevance to the topic were selected. Initially, 24 articles were selected, all published in the last 20 years, in Portuguese and/or English. After analysis, 6 articles matched the proposed objective. Results: It was observed that the difference between PraCO2 at the end of surgery and in the anepathic phase was greater in patients without liver graft dysfunction. A positive correlation was found between ΔpraCO2 and peak ALT after liver transplantation. In another study, it was found that the relationship between poor graft function was the presence of elevated liver enzymes, worsening synthetic liver function, coagulopathy and encephalopathy. It has also been shown that intramucosal gastric pH can predict early graft function. In a group with liver dysfunction, patients had an intramucosal gastric pH of less than 7.3 in the perioperative period, which remained low until the 24th hour postoperatively, while the group without dysfunction had an intramucosal gastric pH of more than 7.3, except in the anepathic phase when it was below this value. Conclusion: The usefulness of gastrointestinal tonometry to monitor splanchnic circulation and liver graft function during liver transplantation has been described and although support for this statement was found in some studies, this review had limitations due to the small number of articles available, which prevents it from covering a wide range of scientific evidence. Introduction Objective databases used Tonometry, Tonometry , “Tonometry” Splanchnic Liver AND, AND “AND” selected Initially 2 years andor or English analysis objective Results PraCO ΔpraCO study enzymes encephalopathy 73 7 3 7. th postoperatively value Conclusion studies available evidence “Tonometry “AND

RESUMO As aves silvestres têm grande destaque na transmissão de doenças ao homem, principalmente pela facilidade de acesso à população humana, levantando preocupações sobre o potencial impacto dessa proximidade no contexto da saúde pública. O presente estudo relata a ocorrência de ARV e PBV em aves silvestres de uma área de proteção ambiental no bioma Amazônico. Obtivemos 155 espécimes fecais de 47 espécies de aves silvestres, que foram submetidos a extração de RNA, detecção de ARV e PBV por técnicas moleculares, sequenciamento nucleotídico e análise filogenética. A prevalência de infecção por ARV foi 0,6% (1/155), sendo positivo em um indivíduo de Myrmotherula longipennis, e a prevalência de PBV foi 1,29% (2/155), afetando dois insdivíduos de Guira guira. A análise filogenética agrupou as cepas de PBV no genogrupo I, mostrando intensa relação filogenética com cepas isoladas de outras regiões geográficas. A cepa de ARV esteve mais relacionada filogeneticamente a cepas previamente circulantes na mesma região. Este é o primeiro estudo sobre detecção de ARV em aves silvestres no Brasil e o primeiro relato sobre a ocorrência de PBV em Guira guira. A circulação de ARV e PBV nesse ambiente demonstra que os ciclos desses agentes estão ocorrendo de forma natural no ecossistema silvestre, o que pode desencadear possíveis eventos de transmissão entre as espécies aviárias e humanos. Estudos adicionais são necessários para determinar a epidemiologia, origem, evolução e surgimento de novos vírus potencialmente patogênicos na Amazônia. homem humana pública Amazônico 15 4 RNA moleculares 06 0 6 0,6 1/155, 1155 1/155 , 1 (1/155) longipennis 129 29 1,29 2/155, 2155 2/155 2 (2/155) guira I geográficas região silvestre humanos epidemiologia origem Amazônia 0, 115 1/15 (1/155 12 1,2 215 2/15 (2/155 11 1/1 (1/15 1, 21 2/1 (2/15 1/ (1/1 2/ (2/1 (1/ (2/ (1 (2 (

ABSTRACT Wild birds have great prominence in the transmission of diseases to humans, mainly due to their ease of access to human populations, raising concerns about the potential impact of that proximity on public health. The present study reports ARV and PBV occurrence in wild birds from an environmental protection area in the Amazon biome, in Pará state, Brazil. We obtained 155 fecal specimens from 47 species of wild birds for RNA extraction, ARV and PBV detection utilizing molecular methods, nucleotide sequencing, and phylogenetic analysis. ARV prevalence was 0.6% (1/155), being positive in an individual of Myrmotherula longipennis, and PBV prevalence was 1.29% (2/155), affecting two individuals of Guira guira. The PBV strains were assigned to genogroup I based on phylogenetic analysis, and they shared a strong phylogenetic link with strains isolated from different geographic locations. The ARV strain was more closely related to strains that had previously circulated in the same region. The presence of ARV and PBV in this habitat suggests that infection cycles of these agents occur naturally in the wild ecosystem, potentially triggering transmission events between bird species and humans. This is the first study on ARV detection in wild birds in Brazil and the first report on the occurrence of PBV in wild Guira guira. Additional studies are required to determine the epidemiology, origin, evolution, and emergence of new potentially pathogenic viruses in the Amazon. humans populations health biome state 15 4 extraction methods sequencing analysis 06 0 6 0.6 1/155, 1155 1/155 , 1 (1/155) longipennis 129 29 1.29 2/155, 2155 2/155 2 (2/155) guira locations region ecosystem epidemiology origin evolution 0. 115 1/15 (1/155 12 1.2 215 2/15 (2/155 11 1/1 (1/15 1. 21 2/1 (2/15 1/ (1/1 2/ (2/1 (1/ (2/ (1 (2 (

Abstract The threatened orchid Cattleya milleri is a microendemic orchid of a Brazilian savanna hotspot. As endophytes and mycorrhizae may improve its propagation and conservation, we investigated its root fungal community. Cattleya milleri roots were sampled in five natural sites and at a greenhouse. Fungal root endophytes were isolated for characterization and molecular ITS (Internal Transcribed Spacer) identification. Total DNA was extracted from endorhiza and rhizosphere for ITS amplification and sequencing. Sixteen fungal isolates were clustered in 6 Operational Taxonomic Units (OTUs) and endorhizal and rhizospheric sequences were clustered in 327 OTUs. Endorhiza presented from 25 to 89 OTUs, and rhizosphere 56 OTUs. Cluster analysis showed high divergence between natural and greenhouse fungal communities, but similarity among natural samples. From the 94 genera, 24 were annotated as endophytes, two mycorrhizas, 33 pathotrophs, 40 saprotrophs, and 17 symbiotrophs based on the FunGuild database. Endophytes of the orders Capnodiales, Hypocreales, Pleosporales, and mycorrhizae of Sebacinales occurred in all sites. The mycorrhizae Tulasnella occurred in all natural samples. The interaction with only two mycorrhizal taxa may limit C. milleri distribution. However, the recruitment of many non-mycorrhizal endophytes is essential to natural development. Pleosporales, Tulasnella, and Sebacinalles may be considered for C. milleri propagation and conservation. hotspot conservation community Internal Spacer identification sequencing OTUs (OTUs 32 2 8 5 communities samples 9 genera mycorrhizas 3 pathotrophs 4 saprotrophs 1 database Capnodiales Hypocreales Pleosporales C distribution However nonmycorrhizal non development

RESUMO Ferimentos penetrantes crânio-orbitário são eventos pouco comuns e representam uma pequena porcentagem dos traumas craniencefálicos. A descrição da condição clínica e o manejo cirúrgico foram realizadas a partir da revisão do prontuário eletrônico do paciente. O presente relato é do caso de um paciente do sexo masculino, de 37 anos, que apresentou trauma penetrante em crânio pelo manete de freio de sua bicicleta, devido à queda acidental enquanto pedalava. Trazido pelo SAMU, o paciente apresentava escore de Glasgow 15, órbita esquerda preservada, enquanto na órbita direita, observou-se ausência de abertura ocular, amaurose, pupila midriática não fotorreagente, ao reflexo direto e indireto. Na tomografia de crânio, visualizou-se manete de freio metálico alojado no crânio à direita, promovendo fratura da face orbital do zigomático e asa maior do esfenoide, com extremidade na órbita. No centro cirúrgico, a extração cuidadosa do objeto foi realizada sem complicações. No primeiro dia de pós-operatório, foi solicitada ressonância magnética que discriminou, do lado direito, contusão hemorrágica do lobo temporal, ruptura do músculo reto lateral, conteúdo hemático e gasoso no interior da órbita e possível descolamento de retina. No sexto dia de pós-operatório, foi mantida amaurose à direita. O paciente recebeu alta e foi encaminhado para acompanhamento oftalmológico especializado. Traumas penetrantes crânio-orbitário apresentam desfechos potencialmente fatais se não diagnosticados e manejados precoce e adequadamente. crânioorbitário orbitário craniencefálicos masculino 3 anos bicicleta pedalava SAMU 15 preservada direita observouse observou ocular fotorreagente indireto visualizouse visualizou esfenoide complicações pósoperatório, pósoperatório pós operatório, operatório pós-operatório discriminou direito temporal lateral retina especializado adequadamente 1

ABSTRACT Penetrating cranio-orbital injuries are uncommon events and represent a small percentage of cranioencephalic traumas. The clinical condition and surgical management were described by reviewing the patient's electronic medical records. This case reports on a 37-year-old male patient who suffered penetrating trauma to the skull from the brake lever of his bicycle as a result of an accidental fall while cycling. Brought in by the SAMU, the patient had a Glasgow score of 15, a preserved left orbit, while the right orbit showed no eye opening, amaurosis, a non-photo-reagent mydriatic pupil, both to the direct and indirect reflex. A computed tomography scan of the skull showed a metal brake handle lodged in the skull on the right, causing a fracture of the orbital face of the zygomatic and greater wing of the sphenoid, with the end in the orbit. In the operating room, the object was carefully extracted without complications. On the first postoperative day, a magnetic resonance imaging scan was requested, which revealed a hemorrhagic contusion of the temporal lobe on the right side, rupture of the lateral rectus muscle, hematic and gaseous contents inside the orbit, as well as a possible retinal detachment. On the sixth post-operative day, with amaurosis maintained on the right, the patient was discharged and referred for specialized ophthalmological follow-up. Penetrating cranio-orbital trauma has potentially fatal outcomes if not diagnosed and managed early and properly. cranioorbital cranio traumas patients s records 37yearold yearold 37 year old cycling SAMU 15 opening nonphotoreagent non photo reagent pupil reflex sphenoid room complications day requested side muscle detachment post operative followup. followup follow up. up follow-up properly 3 1

RESUMO Encontrar caminhos para a agricultura e uso dos recursos mais sustantáveis ainda apresenta o desafio mais urgente para os países amazônicos. Esta revisão caracteriza o status quo e as mudanças recentes na estrutura e nos tipos de sistemas de produção rural, e identifica respostas para lidar com os desafios e oportunidades na promoção de economias extrativistas e agrícolas mais sustentáveis na Amazônia. Enquanto a agricultura regional e economia de recursos se baseiam em uma rica diversidade de produtores, conhecimentos, e sistemas de produção, a expansão do agronegócio chegou a dominar a distribuição de subsidios, o apoio institucional e a infraestrutura logística. Estas tendências estão associadas com a perda e a degradação das florestas, a poluição das águas, pressão e/ou deslocamento de comunidades indígenas e rurais, bem como o incremento nas emissões de gases com efeito de estufa, minando os serviços ecossistêmicos. Analisamos os impactos diversos e complexos das mudanças sócio-econômicas e hidroclimáticas sobre sistemas de produão, nos meio-ambientes e na biodiversidade nos países amazônicos, com um enfoque mais aprofundado sobre os sistema-chave de produção agrária na Amazônia brasileira usando dados dos censos agropecuários de 1995, 2006 e 2017. A análise quantitativa é complementada por uma discussão qualitativa e empiricamente fundadas sobre as mudanças e impactos de diferentes atividades, como estão interligadas, e como diferem entre os vários países amazônicos. Finalmente oferecemos recomendações para a promoção de sistemas de produção e gestão de pequenos agricultores adaptáveis, rentáveis e mais sustentáveis, que reduzam o desmatamento e apoiem as comunidades e economias locais no contexto da crescente urbanização e mudanças climáticas. amazônicos rural produtores conhecimentos subsidios logística florestas águas eou ou rurais estufa ecossistêmicos sócioeconômicas sócio econômicas produão meioambientes meio ambientes sistemachave sistema chave 1995 200 2017 atividades interligadas adaptáveis climáticas 199 20 201 19 2 1

ABSTRACT Finding pathways to more sustainable agriculture and resource use remains the most pressing challenge for Amazonian countries. Characterizing recent changes in the structure and types of agrarian production systems, this review identifies responses to deal with the challenges and opportunities to promote more sustainable production and extraction economies in the Amazon. While regional agriculture and resource economies rest on a rich diversity of producers, knowledge, and production systems, the expansion of agribusiness enterprises has come to dominate the distribution of subsidies, institutional support, and logistical infrastructure. These trends are associated with forest loss and degradation, pollution of waterways, pressures on and/or displacement of indigenous and rural communities, and increased greenhouse gas emissions, all of which undermine ecosystem services. We analyzed the diverse and complex impacts of socio-economic and hydro-climatic changes on livelihoods, environments and biodiversity in Amazonian countries, with a more in-depth focus on changes in key agrarian production systems in the Brazilian Amazon using agrarian census data from 1995, 2006, and 2017. The quantitative analysis is complemented by a qualitative and empirically grounded discussion that provides insights into the changes and impacts of different activities, how they are interlinked, and how they differ across Amazonian countries. Finally, we provide recommendations towards promoting adaptive, profitable, and more sustainable smallholder production and management systems that reduce deforestation and support local communities and economies in the context of increasing urbanization and climate change. countries producers knowledge subsidies infrastructure degradation waterways andor or emissions services socioeconomic socio economic hydroclimatic hydro climatic livelihoods indepth depth 1995 2006 2017 activities interlinked Finally adaptive profitable change 199 200 201 19 20 1 2

Resumo Objetivo Investigar a associação entre a Insegurança Alimentar (IA) e as Doenças Crônicas Não Transmissíveis (DCNT) em pessoas idosas da Estratégia Saúde da Família (ESF). Método Estudo transversal realizado com pessoas idosas de um município do Nordeste do Brasil. A presença de DCNT (Diabetes Mellitus (DM); Hipertensão Arterial Sistêmica (HAS); e dislipidemias) foi investigada por meio do diagnóstico médico autorrelatado (sim ou não). Já a IA foi avaliada pela Escala Brasileira de Insegurança Alimentar (EBIA). No processamento dos dados foi feita a análise descritiva, a análise bivariada com o teste qui-quadrado de Pearson e a regressão logística binomial. O nível de significância foi considerado para p<0,05. Resultados Foram avaliadas 316 pessoas idosas e observou-se uma prevalência de 63,3% para IA (37,7% de IA leve e 25,6% de IA moderada/grave). Em relação às DCNT investigadas, observou-se uma prevalência de 24,1% para DM, 69,9% para HAS e 30,7% para dislipidemia. Na análise de regressão, após ajustes, não houve associação entre IA e HAS ou DM. Houve associação somente entre IA leve e dislipidemia (OR 2,036; IC 95%:1,072 – 3,866). Não foi encontrada associação entre IA moderada/grave e dislipidemia (OR 1,779; IC 95%: 0,877 – 3,610). Conclusão O estudo aponta a IA como um problema social e de saúde pública em pessoas idosas atendidas pela ESF em um município do nordeste brasileiro, tanto pelas prevalências encontradas, quanto pela associação com doenças crônicas. A implementação de políticas públicas específicas para pessoas idosas pode melhorar o acesso a uma alimentação saudável e auxiliar na manutenção da saúde. (IA (DCNT ESF. . (ESF) Brasil Diabetes DM (DM) (HAS) dislipidemias sim não. não) EBIA. EBIA (EBIA) descritiva quiquadrado qui quadrado binomial p005 p 0 05 p<0,05 31 observouse observou se 633 63 3 63,3 37,7% 377 37 7 (37,7 256 25 6 25,6 moderada/grave. moderadagrave moderada grave moderada/grave) investigadas 241 24 1 24,1 699 69 9 69,9 307 30 30,7 ajustes OR 2,036 2036 2 036 95%1,072 951072 95% 1,072 95 072 95%:1,07 3,866. 3866 3,866 866 3,866) 1,779 1779 779 0877 877 0,87 3,610. 3610 3,610 610 3,610) brasileiro encontradas crônicas (ESF (DM (HAS (EBIA p00 p<0,0 63, 37,7 (37, 25, 24, 69, 30, 2,03 203 03 95%1,07 95107 1072 1,07 07 95%:1,0 386 3,86 86 1,77 177 77 087 87 0,8 361 3,61 61 p0 p<0, 37, (37 2,0 20 95%1,0 9510 107 1,0 95%:1, 38 3,8 8 1,7 17 08 0, 36 3,6 p<0 (3 2, 95%1, 951 10 1, 95%:1 3, p< ( 95%1

Abstract Objective To investigate the association between Food Insecurity (FI) and Chronic Non-Communicable Diseases (NCDs) in older adults in the Family Health Strategy (FHS). Method Cross-sectional study carried out with older adults from a city in the Northeast of Brazil. The presence of NCDs (Diabetes mellitus (DM); Systemic Arterial Hypertension (SAH); and dyslipidemia) was investigated through self-reported medical diagnosis (yes or no). FI was assessed using the Brazilian Food Insecurity Scale (EBIA). In data processing, descriptive analysis, bivariate analysis with Pearson's chi-square test and binomial logistic regression were carried out. The significance level was considered at p<0.05. Results 316 older adults were evaluated and a prevalence of 63.3% for FI was observed (37.7% for mild FI and 25.6% for moderate/severe FI). Regarding the NCDs investigated, a prevalence of 24.1% for DM, 69.9% for hypertension and 30.7% for dyslipidemia was observed. In the regression analysis, after adjustments, there was no association between FI and SAH or DM. There was only an association between mild FI and dyslipidemia (OR 2.036; 95% CI: 1.072 – 3.866). No association was found between moderate/severe FI and dyslipidemia (OR 1.779; 95% CI: 0.877 – 3.610). Conclusion The study points to FI as a social and public health problem in older adults cared for by the FHS in a municipality in northeastern Brazil, both due to the prevalence found and the association with chronic diseases. The implementation of specific public policies for older adults can improve access to healthy food and help maintain health. (FI NonCommunicable Non Communicable (NCDs FHS. . (FHS) Crosssectional Cross sectional Brazil Diabetes DM (DM) (SAH) selfreported self reported yes no. no) EBIA. EBIA (EBIA) processing Pearsons Pearson s chisquare chi square p005 p 0 05 p<0.05 31 633 63 3 63.3 37.7% 377 37 7 (37.7 256 25 6 25.6 moderatesevere moderate severe FI. FI) 241 24 1 24.1 699 69 9 69.9 307 30 30.7 adjustments OR 2.036 2036 2 036 95 CI 1072 072 1.07 3.866. 3866 3.866 866 3.866) 1.779 1779 779 0877 877 0.87 3.610. 3610 3.610 610 3.610) diseases (FHS (DM (SAH (EBIA p00 p<0.0 63. 37.7 (37. 25. 24. 69. 30. 2.03 203 03 107 07 1.0 386 3.86 86 1.77 177 77 087 87 0.8 361 3.61 61 p0 p<0. 37. (37 2.0 20 10 1. 38 3.8 8 1.7 17 08 0. 36 3.6 p<0 (3 2. 3. p< (