RESUMO O objetivo foi descrever os níveis séricos de progesterona (P4) de éguas tratadas com um ou dois dispositivos intravaginais de primeiro uso e com um dispositivo de segundo uso de P4, em um período de 10 dias e comparar a concentração de P4. Três éguas foram tratadas com implante intravaginal de P4 (Reprosync®; 2g de P4) por 10 dias (D0 a D10). A égua 1 recebeu dois implantes novos, a égua 2 recebeu um implante novo e a égua 3 recebeu um implante usado de P4. Coletas de sangue foram realizadas diariamente (a.m./p.m.) do D0 ao D10 e concentrações de P4 foram analisadas por quimioluminescência. A égua que recebeu dois dispositivos apresentou numericamente maior concentração sérica média de P4 (5,11±2,24ng/mL) do que a égua que recebeu um dispositivo novo (3,27±0,94) e do que a égua que recebeu um dispositivo usado (2,78±0,72). As éguas que receberam um dispositivo (novo ou usado) tiveram médias de P4 relativamente semelhantes numericamente. Concluiu-se que a concentração sérica de P4 de éguas recebendo diferentes quantidades de dispositivos intravaginais de P4 parece diferir de acordo com o número de implantes inseridos. Ainda assim, todos os tratamentos atingiram concentrações séricas de P4 capazes de bloquear a ovulação. P (P4 Reprosync® Reprosync (Reprosync® g D (D D10. . D10) novos a.m./p.m. ampm m p (a.m./p.m. D1 quimioluminescência 5,11±2,24ng/mL 511224ngmL ngmL 5 11 24ng mL ng (5,11±2,24ng/mL 3,27±0,94 327094 27 0 94 (3,27±0,94 2,78±0,72. 278072 2,78±0,72 78 72 (2,78±0,72) Concluiuse Concluiu se inseridos assim ovulação (P (Reprosync a.m./p.m (a.m./p.m 3,27±0,9 32709 9 (3,27±0,9 27807 2,78±0,7 7 (2,78±0,72 3,27±0, 3270 (3,27±0, 2780 2,78±0, (2,78±0,7 3,27±0 327 (3,27±0 278 2,78±0 (2,78±0, 3,27± 32 (3,27± 2,78± (2,78±0 3,27 (3,27 2,78 (2,78± 3,2 (3,2 2,7 (2,78 3, (3, 2, (2,7 (3 (2, ( (2

Resumo Esse estudo objetivou avaliar as injúrias dentárias traumáticas (IDTs) na dentição permanente entre os pacientes que compareceram ao ambulatório de uma faculdade de odontologia brasileira, durante os últimos 20 anos, e investigar fatores associados à severidade dessas lesões. Os registros clínicos dos pacientes que compareceram a um centro especializado de atendimento em traumatismo dentário no Brasil apresentando pelo menos uma IDT em dente permanente, entre os anos de 2000 e 2019, foram revisados. Os dados registrados foram sexo, idade, arco dental afetado, etiologia, número e tipo dos dentes afetados, e classificação e severidade das IDTs. O diagnóstico e a classificação das IDTs foram baseados nas diretrizes da Associação Internacional de Traumatologia Dentária (AITD). A gravidade das lesões de cada paciente foi definida como leve, moderada ou severa. Estatísticas descritivas, teste qui-quadrado e análises de regressão multinomial foram usadas para avaliar os resultados. O nível de significância foi fixado em 5%. Um total de 837 registros clínicos foi incluído, totalizando 2357 dentes. O sexo masculino foi mais prevalente que o feminino. A idade dos pacientes variou de 5 a 71 anos. Os traumas mais comuns foram avulsão (n=512) e fratura não-complicada do esmalte-dentina (n=488). As análises univariadas mostraram que houve associação estatisticamente significativa entre a faixa etária (p=0,004), etiologia (p=0,000) e número de dentes afetados (p=0,000) com a gravidade do traumatismo dentário. Em conclusão, as IDTs que ocorreram em Piracicaba e região são epidemiologicamente semelhantes aos encontrados em todo o mundo, e que lesões mais graves estão relacionadas à faixa etária, etiologia e número de dentes afetados. (IDTs brasileira 2 200 2019 revisados afetado AITD. AITD . (AITD) leve severa descritivas quiquadrado qui quadrado resultados 5% 83 incluído 235 feminino 7 n=512 n512 n 512 (n=512 nãocomplicada não complicada esmaltedentina esmalte dentina n=488. n488 n=488 488 (n=488) p=0,004, p0004 p p=0,004 , 0 004 (p=0,004) p=0,000 p0000 000 (p=0,000 conclusão mundo 201 (AITD 8 23 n=51 n51 51 (n=51 n48 n=48 48 (n=488 p000 p=0,00 00 (p=0,004 (p=0,00 n=5 n5 (n=5 n4 n=4 4 (n=48 p00 p=0,0 (p=0,0 n= (n= (n=4 p0 p=0, (p=0, (n p=0 (p=0 p= (p= (p

Abstract This study aimed to assess the traumatic dental injuries (TDIs) in permanent dentition among patients who attended at the outpatient clinic of a Brazilian dental school, during the last 20 years, and to investigate factors associated with the severity of these injuries. Clinical records of patients who attended a specialized center for dental trauma care in Brazil presenting at least one TDI in a permanent tooth, between the years 2000 and 2019, were reviewed. The data recorded were sex, age, affected arch, etiology, number, and type of the teeth affected, and classification and severity of the TDIs. The diagnosis and classification of the TDIs were based on the guidelines of the International Association of Dental Traumatology (IADT). The severity of each patient's injuries was defined as mild, moderate, or severe. Descriptive statistics, chi-square and multinomial regression analyses were used to evaluate the results. The significance level was set at 5%. A total of 837 clinical records were included, totaling 2357 teeth. Males were more prevalent than females. The patients' age ranged from 5 to 71 years. The most common traumas were avulsion (n=512) and uncomplicated enamel-dentin fracture (n=488). Univariate analyses showed that there was a statistically significant association between age group (p=0.004), etiology (p=0.000) and number of teeth affected (p=0.000) with severity of dental trauma. In conclusion, TDIs that occurred in Piracicaba and region are epidemiologically similar to those found worldwide, and that more severe injuries are related to age range, etiology and number of teeth affected. (TDIs school 2 tooth 200 2019 reviewed sex arch IADT. IADT . (IADT) patient s mild moderate statistics chisquare chi square results 5% 83 included 235 females 7 n=512 n512 n 512 (n=512 enameldentin enamel dentin n=488. n488 n=488 488 (n=488) p=0.004, p0004 p p=0.004 , 0 004 (p=0.004) p=0.000 p0000 000 (p=0.000 conclusion worldwide range 201 (IADT 8 23 n=51 n51 51 (n=51 n48 n=48 48 (n=488 p000 p=0.00 00 (p=0.004 (p=0.00 n=5 n5 (n=5 n4 n=4 4 (n=48 p00 p=0.0 (p=0.0 n= (n= (n=4 p0 p=0. (p=0. (n p=0 (p=0 p= (p= (p

Abstract There is an increasing interest in essential oils extracted from Verbenaceae plant species as potential sources of biologically active compounds that could provide a starting point for designing novel phyto-pharmaceuticals in aquaculture. The present study was aimed to investigate the chemical composition, antioxidant activity, acute toxicity and antimicrobial effects against Vibrio parahaemolyticus of essential oils extracted from Lippia alba and L. origanoides. Approximately 23 components were identified and quantified by gas chromatography-mass spectrometry and flame ionization detection in each species’ essential oil. The most predominant compounds were geranial (23.0%), limonene (17.0%) and neral (15.5%) in L. alba, and thymol (47.2%), p-cymene (16.0%) and E-caryophyllene (11.3%) in L. origanoides. The essential oils have antibacterial activity against Vibrio parahaemolyticus presenting Minimum Inhibitory Concentration (MIC) and Bactericidal Concentration (MBC) values between 156-625 µg mL-1. The essential oils also show antioxidant potential estimated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assays, presenting IC50 of 60.16 mg mL-1 and 0.22 mg mL-1 for L. alba and L. origanoides EO, respectively. Both oils were classified as toxic to Artemia salina nauplii. Therefore, these essential oils may be useful for controlling pathogenic bacteria important to the aquaculture industry. phytopharmaceuticals phyto pharmaceuticals composition L 2 chromatographymass chromatography mass oil 23.0%, 230 23.0% , 0 (23.0%) 17.0% 170 17 (17.0% 15.5% 155 15 5 (15.5% 47.2%, 472 47.2% 47 (47.2%) pcymene p cymene 16.0% 160 16 (16.0% Ecaryophyllene E caryophyllene 11.3% 113 11 3 (11.3% MIC (MIC MBC (MBC 156625 156 625 156-62 mL1. mL1 mL 1. 1 1,1diphenyl2picrylhydrazyl 11diphenyl2picrylhydrazyl diphenylpicrylhydrazyl 1,1 diphenyl picrylhydrazyl DPPH (DPPH assays IC IC5 6016 60 60.1 mL- 022 22 0.2 EO respectively nauplii Therefore industry 23.0 (23.0% 17.0 (17.0 15.5 (15.5 47.2 4 (47.2% 16.0 (16.0 11.3 (11.3 15662 62 156-6 1diphenyl2picrylhydrazyl 1, 601 6 60. 02 0. 23. (23.0 17. (17. 15. (15. 47. (47.2 16. (16. 11. (11. 1566 156- (23. (17 (15 (47. (16 (11 (23 (1 (47 (2 ( (4

RESUMO O estudo teve como objetivo avaliar características espermáticas in vitro e a taxa de concepção (TC) de sêmen convencional e sexado em um programa de IATF tradicional de vacas Nelore pós-parto. Todas a vacas (n=348) foram submetidas ao mesmo protocolo de IATF à base de estradiol e de progesterona. Após 48 horas da retirada do implante, foi determinada a expressão de estro dos animais e a IA foi realizada com sêmen convencional e sexado de dois touros Angus. As seguintes características espermáticas foram avaliadas: análise computadorizada do sêmen, teste hiposmótico, morfometria espermática e estrutura cromatínica por meio da coloração com azul de toluidina. A TC foi menor (P<0,001) para sêmen sexado comparado ao convencional, em vacas que expressaram estro (27 vs. 47%) e que não apresentaram estro (11 vs. 37%). A integridade da membrana plasmática (Touro 1: 30,3±9,6 vs. 52,3±12,4%, P=0,010; Touro 2: 24,5±3,0 vs. 48,7±1,6%, P=0,006) e a concentração espermática (Touro 1: 23,2±0,6 vs. 43,0±0,8x10⁶sperm/mL, P<0,001; Touro 2: 25,1±2,8 vs. 42,1±0,7x10⁶sperm/mL, P<0,001) foram menores no sêmen sexado comparado ao convencional, para ambos os touros. Motilidades total e progressiva foram menores no sêmen sexado comparado ao convencional para o Touro 1 (MT: 49,7±15,9 vs. 94,9±1,9%, P=0,007; MP: 16,7±3,4 vs. 44,1±13,2%, P=0,009), enquanto diferenças não foram detectadas no Touro 2 (MT: 45,0±17,5 vs. 68,2±19,1%, P=0,098; MP: 12,8±4,7 vs. 30,0±13,0%, P=0,065). Elipticidade espermática do sêmen sexado foi menor do que do sêmen convencional no Touro 2 (0,306±0,01 vs. 0,342±0,02, P=0,020), mas não houve diferença no Touro 1 (0,332±0,01 vs. 0,330±0,01, P=0,552). Reduzida fertilidade in vivo foi observada para o sêmen sexado em relação ao convencional, independentemente da expressão de cio das vacas. A qualidade seminal in vitro do sêmen sexado foi comprometida para ambos os touros, mas diferentemente afetada para cada reprodutor.

ABSTRACT The aim of this study was to assess in vitro sperm characteristics and pregnancies/AI (P/AI) of conventional and sex-sorted semen at timed-AI of suckled, multiparous Nelore cows. All cows (n=348) were submitted to a traditional estradiol/progesterone(P4)-based protocol. At 48h after P4-device removal, the estrous behavior was recorded, and AI was performed with conventional or sex-sorted semen from two bulls. The following sperm assessments were performed: CASA, Hyposmotic Test, sperm morphometry and chromatin structure by TB staining. P/AI were reduced (P<0.001) for sex-sorted compared to conventional semen in cows expressing estrus (27vs47%) or not (11vs.37%). Membrane integrity (Bull1: 30.3±9.6 vs. 52.3±12.4%, P=0.01; Bull2: 24.5±3.0 vs. 48.7±1.6%, P=0.006) and sperm concentration (Bull1: 23.2±0.6 vs. 43.0±0.8x10⁶sperm/mL, P<0.001; Bull2: 25.1±2.8 vs. 42.1±0.7x10⁶sperm/mL; P<0.001) were reduced in sex-sorted compared to conventional semen, for both bulls. Total and progressive motility were reduced in sex-sorted semen for Bull1 (TM: 49.7±15.9 vs. 94.9±1.9%, P=0.007; PM: 16.7±3.4 vs. 44.1±13.2%, P=0.009) and no differences were detected for Bull2 (TM: 45.0±17.5 vs. 68.2±19.1%, P=0.098; PM: 12.8±4.7 vs. 30.0±13.0%, P=0.065). Sperm ellipticity from sex-sorted was lower than conventional semen for Bull2 (0.306±0.01 vs. 0.342±0.02, P=0.02) and no difference was detected for Bull1 (0.332±0.01 vs. 0.330±0.01, P=0.55). Reduced in vivo fertility was observed for sex-sorted semen, regardless of estrous behavior. In vitro sperm quality of sex-sorted semen was compromised for both bulls, but differently affected for each sire.

RESUMO O objetivo deste trabalho foi avaliar a taxa de crescimento folicular e a taxa ovulatória em éguas tratadas com dispositivo intravaginal de progesterona (P4) durante o período de 10 dias, associado à utilização de benzoato de estradiol (BE). Os resultados foram comparados durante o período de transição (ET) da primavera com a época de reprodução no verão (ER). As variáveis foram submetidas à ANOVA (teste de Tukey), considerando-se P<0,05. Nenhuma ovulação ocorreu durante a permanência do dispositivo de P4 em ambos os períodos experimentais. A taxa ovulatória na ER foi de 100% (n = 8) e na ET, de 62,5% (n=5; P=0,0547). Diferença significativas (<0,001) foram observadas, em ambos os períodos experimentais, comparando as taxas de crescimento folicular durante a permanência da P4 (ER: 1,33 ± 0,89mm/d; ET: 1,00 ± 0,81mm/d) com o período sem P4 (ER: 3,63 ± 1,33mm/d; ET: 3,31 ± 1,66mm/d). O presente estudo demonstrou aplicabilidade e eficiência do protocolo hormonal utilizando dispositivo intravaginal de P4 e BE para controle folicular de éguas, tanto na ET quanto na ER.

ABSTRACT The objective of this study was to evaluate follicular growth and ovulatory rates in mares treated with an intravaginal progesterone device (P4) during the 10-day period, associated with the use of estradiol benzoate (EB). The results were compared during the transition period (ET) in the spring and the breeding season in the summer (ER). The variables were submitted to ANOVA (Tukey's test), considering P<0.05. No ovulation occurred during the permanence of the P4 implant in both experimental periods. The ovulatory rate in the ER was 100% (n = 8) and in the ET 62.5% (n = 5; P = 0.0547). Significant differences were observed (<0.001), in both periods, comparing follicular growth rates during the permanence of P4 device (ER: 1.33 ± 0.89mm/d; ET: 1.00 ± 0.81mm/d) to the period without P4 (ER: 3.63 ± 1.33 mm/d; ET: 3.31 ± 1.66 mm/d). The present study demonstrated applicability and efficiency of a hormonal protocol using P4 intravaginal device and EB for follicular control in mares, both during ET and ER.

RESUMO: O uso da soja transgênica resistente ao glyphosate pode causar impactos diretos ou indiretos nos componentes do agroecossistema. O impacto direto é provocado pela inserção do gene de resistência, e o indireto está relacionado às alterações nas práticas de manejo. Dessa forma, este trabalho objetivou avaliar o impacto da soja resistente ao glyphosate e seu manejo com esse herbicida sobre a comunidade de artrópodes do dossel das plantas. Os tratamentos foram: soja transgênica e não transgênica com capina mecânica das plantas daninhas; e soja transgênica com uma e três aplicações de glyphosate. As populações de artrópodes no dossel das plantas foram amostradas ao longo de dois cultivos. A incorporação do gene de resistência ao glyphosate não afetou a riqueza e a abundância de artrópodes no dossel das plantas. Já a aplicação do glyphosate reduziu a riqueza de predadores e de fitófagos mastigadores e sugadores nos tratamentos com três aplicações desse herbicida. No segundo ano de cultivo a densidade total de artrópodes foi menor na soja transgênica com três aplicações de glyphosate em relação aos demais tratamentos. O mesmo ocorreu com a densidade do fitófago mastigador Cerotoma arcuatus nos dois anos de cultivo. Já as densidades dos fitófagos succionadores Bemisia tabaci, Caliothrips brasiliensis e Tetranychus sp. foram maiores na soja transgênica com três aplicações de glyphosate do que nos demais tratamentos. A aplicação de glyphosate (uma ou três) reduziu densidades do predador Solenopsis sp. e do detritívoro Hypogastrurasp.

ABSTRACT: This study aims to evaluate the impact of glyphosate-resistant soybean and its management with glyphosate on the canopy arthropod community. We study the direct impact of the insertion of the resistance gene and the indirect impact of management practices, specifically herbicide application. To do this, we use the following treatments: non-GM and GM soybean with mechanical weed control and GM soybean with one and three applications of glyphosate. Arthropods in the crop canopy were sampled over time in all treatments. The incorporation of the glyphosate resistance gene did not affect the richness and abundance of arthropods in the plant canopy. Glyphosate application reduced the richness of predators and chewing and sucking phytophagous arthropods in treatments with three herbicide applications. In the second season, total arthropod density was the lowest in transgenic soybean with three glyphosate applications. The density of Cerotoma arcuatus, a chewing phytophagous arthropod, followed similar trends, while both glyphosate treatments (one or three applications) reduced the densities of the predator Solenopsis sp. and the detritivore Hypogastrura sp. Meanwhile, the densities of the sucking phytophagous arthropods Bemisia tabaci, Caliothrips brasiliensis, and Tetranychus sp. were the highest in transgenic soybean with three glyphosate applications.

The objective of this study was to evaluate the effects of various concentrations of sodium chloride (NaCl), magnesium chloride (MgCl2), and calcium chloride (CaCl2) on the growth and in vitro fermentation of cellulolytic, glycolytic, and amylolytic microorganisms from the rumen of a goat. Six concentrations of each salt were tested separately, namely 0 mg/dL, 100 mg/dL, 200 mg/dL, 400 mg/dL, 800 mg/dL, and 1600 mg/dL in the culture medium. The experiments were conducted in a completely randomized design, in a 6 x 3 factorial arrangement of salt concentration and substrate (starch, cellulose, and glucose) with three replications of each treatment combination. Concentrations of microbial protein, ammonia (NH3-N), and volatile fatty acids (acetate, propionate, and butyrate) were measured. A quadratic effect of CaCl2 concentration on the production of microbial protein was observed in the cellulose medium. The effect of MgCl2 on NH3-N production in the cellulose medium decreased linearly. Propionate concentration decreased linearly with increasing levels of NaCl and MgCl2 in the media containing starch. There was a decreasing linear effect of MgCl2 on the concentration of butyrate in the media containing glucose. In conclusion, concentrations of NaCl and CaCl2 up to 1,600 mg/dL did not affect the microbial activity of starch, cellulose, and glucose-fermenting organisms. However, the microbial activity of starch-fermenting microbes was inhibited at salt concentrations above 800 mg/dL. Thus, brackish water could be used by goats in semiarid regions, but its use should be managed carefully so that it does not have a negative impact on rumen microbial populations.

This study aimed to evaluate the microbial populations, fermentation profile, dry matter recovery and chemical composition of elephant grass silage with ground maize (GM) and the fermented juice of epiphytic lactic acid bacteria (FJLB). A factorial design was used with four levels of GM (0 g/kg, 50 g/kg, 100 g/kg and 200 g/kg), untreated or treated with FJLB, in a completely randomized design with five replicates. A pre-experiment was undertaken to determine the optimum level of sucrose to be added to fermented juice for the development of epiphytic microflora. In this pre-experiment, a completely randomized design with three replications was used. The treatments were represented by the levels of sucrose (0 g/kg, 5 g/kg, 10 g/kg, 20 g/kg, 40 g/kg, 60 g/kg and 80 g/kg, fresh matter basis). The microbial populations, dry matter recovery, and effluent losses were affected by the interaction between GM and FJLB. Dry matter (DM) and crude protein (CP) contents increased linearly with the inclusion of GM. The addition of GM enhanced the fermentation process via a reduction in losses, and improvements were identified in the nutritional value of elephant grass silages. The use of fermented juice increased dry matter recovery, and its effect was more pronounced when ground maize was added.

RESUMO Objetivou-se avaliar o desempenho bioeconômico de bezerros, nos primeiros 60 dias de vida, submetidos a três sistemas de aleitamento. Foram utilizados 24 bezerros (Holandês x Guzerá), sendo 12 machos e 12 fêmeas, com peso inicial de 32,25±4,8kg para as fêmeas e 36,92±6,8kg para os machos. Os animais foram distribuídos em delineamento inteiramente ao acaso, em esquema fatorial (3 x 2). Os bezerros receberam água à vontade e seis litros de sucedâneo lácteo por dia, durante 60 dias, em três estratégias diferentes, denominadas sistema de aleitamento (SA30: 3 litros de sucedâneo lácteo, duas vezes ao dia, até 30 dias de idade; SA45: 3 litros de sucedâneo lácteo, duas vezes ao dia, até 45 dias de idade; SA49: 3 litros de sucedâneo lácteo, duas vezes ao dia, até 49 dias de idade). Os sistemas de aleitamento estudados não apresentaram diferença estatística (P>0,05) para o consumo e a digestibilidade de nutrientes, com exceção para o consumo de matéria orgânica (MO) e extrato etéreo (EE). Verificou-se interação (P<0,05) entre o sistema de aleitamento e a classe sexual para os consumos de MO e EE, bem como para o ganho médio diário, em que os machos do SA 49 apresentaram maiores médias em relação ao SA 30. O desempenho bioeconômico de bezerros machos do sistema de aleitamento 49 foi superior e apresentou a melhor relação custo-benefício entre os sistemas estudados.

ABSTRACT The objective of this study was to evaluate the bioeconomic performance of calves in the first 60 days of life submitted to three feeding systems. Twenty-four calves (Dutch x Guzerá) were used, 12 males and 12 females, with initial weight of 32.25±4.8kg for females and 36.92±6.8kg for males. The animals were distributed in a completely randomized design, in a factorial scheme (3 x 2). The calves received water at will and six liters of milk replacer a day for 60 days in three different strategies, called the suckling system (SA-30: 3 liters of milk replacer, twice a day until 30 days of age; SA-45: 3 liters of milk replacer, twice a day until 45 days of age; SA-49: 3 liters of milk replacer, twice daily up to 49 days old). The lactation systems studied did not present statistical difference (P>0.05) for the consumption and digestibility of nutrients, except for organic matter (OM) and ethereal extract (EE). There was an interaction (P<0.05) between the suckling system and sexual class for the OM and EE intakes, as well as for the average daily gain, in which HS 49 males presented higher averages in relation to SA 30. The bioeconomic performance of male calves from the lactation system 49 was superior and presented the best cost-benefit ratio among the systems studied.

Pituitary adenomas account for 10–15% of primary intracranial tumors. Growth hormone (GH)-secreting adenomas account for 13% of all pituitary adenomas and cause acromegaly. These tumors can be aggressive, invade surrounding structures and are highly recurrent. The objective of this study was to evaluate E-cadherin, Slug and neural cell adhesion molecule (NCAM) expression in GH-secreting pituitary adenomas and its relationship to tumor invasiveness. A cross–sectional study of patients who underwent hypophysectomy due to GH-secreting pituitary adenoma from April 2007 to December 2014 was carried out. The medical records were reviewed to collect clinical data. Immediately after surgery, tumor samples were frozen in liquid nitrogen and stored in a biofreezer at –80°C for assessment of E-cadherin 1 (CDH1), SLUG (SNAI2), and NCAM (NCAM1) by real-time PCR. The samples were fixed in formalin and embedded in paraffin for immunohistochemical analysis of E-cadherin and NCAM. Thirty-five patients with acromegaly were included in the study. Of these, 65.7% had invasive tumors. Immunohistochemically, E-cadherin was expressed in 96.7% of patients, and NCAM in 80% of patients. There was no statistically significant relationship between tumor grade or invasiveness and immunohistochemical expression of these markers. Regarding gene expression, 50% of cases expressed CDH1, none expressed SNAI2, and 53.3% expressed NCAM1. There was no statistically significant relationship between tumor grade or invasiveness and gene expression of CDH1, SNAI2, and NCAM1. The absence of Slug overexpression and of E-cadherin and NCAM suppression suggests that expression of these markers is not associated with tumor invasiveness in GH-secreting pituitary adenomas.

RESUMO Objetivou-se avaliar a influência de dietas contendo silagem de diferentes cultivares de sorgo sobre as características físicas e químicas na carne de ovinos sem padrão racial definido, com idade entre cinco e sete meses e peso vivo médio de 17,7±3,7kg, alimentados com dietas contendo silagem de diferentes cultivares de sorgo. O experimento foi conduzido na Estação Experimental Pendência, pertencente à Empresa Estadual de Pesquisa Agropecuária - Emepa, tendo um período experimental de 42 dias. Utilizou-se um delineamento inteiramente ao acaso, com cinco tratamentos (silagem de sorgo BRS 610, BRS 655, BRS 800, BRS 810 e Ponta Negra) e sete repetições. Ao atingirem o peso de abate de 26,24kg, os animais foram encaminhados ao abate. Procedeu-se à análise do pH, da cor, da perda de peso por cocção, da força de cisalhamento, bem como à determinação de umidade, proteínas, cinzas e lipídeos totais no músculo Longissimus dorsi. As dietas não influenciaram os parâmetros de pH (5,64), cor (L*23,13, a* 18,00, b*15,78), perdas de peso por cocção (34,43%), umidade (72,53%), proteína (24,08%), cinzas (1,09%) e lipídeos (2,99%). A força de cisalhamento diferiu entre as dietas, com a silagem do cultivar BRS 655 propiciando uma carne mais macia (2,10kgf/cm2). As silagens de sorgo utilizadas na confecção das dietas de cordeiros em confinamento não interferem nas características de qualidade da carne de ovinos sem padrão racial definido. Dietas contendo silagem de sorgo granífero BRS 655 proporcionaram uma carne mais macia.

ABSTRACT This study aimed to evaluate the influence of diets containing silage of different sorghum cultivars on the physical and chemical characteristics in the meat of lambs. Thirty five animals of undefined breed with average age between 5 and 7 months and average body weight of 17. 7±3.7kg were used. The lambs were randomly assigned to five treatments (sorghum silage BRS 610, BRS 655, BRS 800, BRS 810 e Ponta Negra) with seven replicates. When they reach 26.24kg of weight, the animals were sent to be slaughtered. Proceeded to the analysis of pH, color, cooking losses, shear force, determination of humidity, protein, ash and total amount of lipids in the Longissimus dorsi. The diets did not influence the parameters of pH (5.64), color (L*23.13, a* 18.00, b*15.78), cooking losses (34.43%), humidity (72.53%), protein (24.08%), ash (1.09%) or lipids (2.99%). The shear force differed among the diets, with the silage of cultivar BRS 655 providing a tender meat (2.10kgf/cm2). The sorghum silages used in in the diets did not interfere in the meat quality characteristics of feedlot lambs. Diets containing sorghum grain silage BRS 655 provided a tender meat.

Although acute exercise is apparently pro-inflammatory and increases oxidative stress, it can promote the necessary stress stimulus to train chronic adaptations in patients with chronic heart failure (CHF). This study aimed to compare the effects of exercise intensity and duration on the inflammatory markers soluble tumor necrosis factor receptor (sTNFR1) and interleukin-6 (IL-6), and on oxidative stress [malondialdehyde (MDA) and antioxidant enzymes: catalase (CAT) and superoxide dismutase (SOD)] in individuals with CHF. Eighteen patients performed three exercise sessions: 30 min of moderate-intensity (M30) exercise, 30 min of low-intensity (L30) exercise, and 45 min of low-intensity (L45) exercise. Blood analysis was performed before exercise (baseline), immediately after each session (after), and 1 h after the end of each session (1h after). Thirty min of M30 exercise promoted a larger stressor stimulus, both pro-inflammatory and pro-oxidative, than that promoted by exercises L30 and L45. This was evidenced by increased sTNFR1 and MDA levels after exercise M30. In response to this stressor stimulus, 1 h after exercise, there was an increase in IL-6 and CAT levels, and a return of sTNFR1 to baseline levels. These findings suggest that compared with the duration of exercise, the exercise intensity was an important factor of physiologic adjustments.

No presente trabalho, objetivou-se avaliar a época de colheita e a qualidade fisiológica de sementes de coentro produzidas no Norte de Minas Gerais. A primeira época de colheita das sementes foi realizada aos 15 dias após o florescimento pleno, quando aproximadamente 50% das plantas apresentavam flores. As demais colheitas foram realizadas de 7 em 7 dias, até as sementes atingirem 14,0% de umidade, fato que ocorreu na 6ª colheita. Imediatamente após cada colheita, as sementes foram avaliadas quanto ao teor de água, à germinação e ao vigor (testes de primeira contagem, emergência de plântulas e índice de velocidade de emergência). A maturidade fisiológica das sementes de coentro, cultivar Verdão, ocorre entre 42 a 44 dias após o florescimento, quando as sementes apresentam umidade em torno de 28,0%, podendo a colheita ser realizada até 50 dias após o florescimento, quando as sementes apresentavam 14,0% de umidade.

The present study aimed to evaluate the harvest season and physiological quality of coriander seeds produced in the North of Minas Gerais. The first season of harvest of the seeds was performed 15 days after full bloom, when approximately 50% of plants had flowers. The other crops were carried out 7 in 7 days, until the seeds reach 14,0% moisture, a fact that occurred in the 6th harvest. Immediately after each harvest, the seeds were evaluated for water content, germination and vigor tests (first count, seedling emergence and emergence speed index). The physiological maturity of coriander seeds, cultivate Verdão, occurs between 42 to 44 days after flowering, when the seeds had humidity around 28,0% at physiological maturity, the harvest may be held until 50 days after flowering, when seeds had 14,0% humidity.

Várias plantas têm sido consideradas produtos terapêuticos, dentre elas destacam-se os chás verde e preto, popularmente utilizados para controle da hiperglicemia e obesidade. Objetivou-se neste trabalho avaliar o potencial inibitório sobre as enzimas α-amilase, α e β-glicosidases e o teor de compostos fenólicos do chá verde e do chá preto. O teor de compostos fenólicos encontrados foram de 80,8 ± 0,43 mg g-1 no chá preto e 32,0 ± 0,12 mg g-1 no chá verde. O chá verde e o chá preto, em condições de consumo, inibiram as enzimas em estudo, porém, após a simulação do fluido gástrico o inibidor presente no chá verde perdeu a ação. O chá preto deixou de inibir a α-amilase e apresentou inibição inalterada para α e β-glicosidases. Tais resultados sugerem que o chá preto pode ser auxiliar em dietas de restrição de carboidratos.

Several plants have been considered therapeutic products, including green and black tea, popularly used to control hyperglycemia and obesity. This study aimed to evaluate the inhibitory potential of the enzymes α-amylase, α and β-glycosidases, as well as the content of phenolic compounds in green tea and black tea. The concentrations of phenolic compounds found were 80.8 ± 0.43 mg g-1 in black tea and 32.0 ± 0.12 mg g-1 in green tea. Under the tested conditions of use, green and black tea inhibited the enzyme under study. However, after simulation of the gastric fluid, the inhibitor present in green tea lost its action. Black tea no longer inhibited a-amylase and showed unaltered inhibition for α and β-glycosidases. These results suggest that black tea can be helpful in diets restricting carbohydrates.

Invasive diseases caused by Corynebacterium diphtheriae have been described increasingly. Several reports indicate the destructive feature of endocarditis attributable to nontoxigenic strains. However, few reports have dealt with the pathogenicity of invasive strains. The present investigation demonstrates a phenotypic trait that may be used to identify potentially invasive strains. The study also draws attention to clinical and microbiological aspects observed in 5 cases of endocarditis due to C. diphtheriae that occurred outside Europe. Four cases occurred in female school-age children (7-14 years) treated at different hospitals in Rio de Janeiro, Brazil. All patients developed other complications including septicemia, renal failure and/or arthritis. Surgical treatment was performed on 2 patients for valve replacement. Lethality was observed in 40% of the cases. Microorganisms isolated from 5 blood samples and identified as C. diphtheriae subsp mitis (N = 4) and C. diphtheriae subsp gravis (N = 1) displayed an aggregative adherence pattern to HEp-2 cells and identical one-dimensional SDS-PAGE protein profiles. Aggregative-adhering invasive strains of C. diphtheriae showed 5 distinct RAPD profiles. Despite the clonal diversity, all 5 C. diphtheriae invasive isolates seemed to display special bacterial adhesive properties that may favor blood-barrier disruption and systemic dissemination of bacteria. In conclusion, blood isolates from patients with endocarditis exhibited a unique adhering pattern, suggesting a pathogenic role of aggregative-adhering C. diphtheriae of different clones in endocarditis. Accordingly, the aggregative-adherence pattern may be used as an indication of some invasive potential of C. diphtheriae strains.