Our study evaluated how the consumption of diets with low (LOW group - 0.4/1) or high (CON group - 13.6/1) omega-6/omega-3 ratio across generations (F1 and F2) can modulate liver fatty acid (FA) profile and blood biomarkers. Liver content of α-linolenic acid was higher in animals always fed with LOW diet than animals that changed from CON to LOW diet, which by your time was higher than animals always fed with CON diet. Liver saturated FA concentration decreased in both groups from F1 to F2. In conclusion, both diets were efficient in decreasing the saturated FA liver content across generations, the LOW ratio diet was more effective in reducing blood triglycerides and non-esterified fatty acids, and there was a multigenerational effect of the LOW ratio diet, improving the FA profile even when the offspring start receiving the CON diet.

O objetivo desse estudo foi comparar a atividade mioelétrica (EMG) do glúteo máximo e bíceps femoral entre o agachamento com passada à frente e com pés paralelos. Sete indivíduos realizaram oito repetições do agachamento paralelo (AP) e com passada à frente (AF) deslocando sobrecarga relativa a 50% da massa corporal. O EMG do glúteo máximo e bíceps femoral foi capturado, filtrado por um filtro passa-banda Butterworth de 4ª ordem (20-400 Hz) e obtido valores RMS. Utilizou-se o teste de Wilcoxon para comparar o EMG normalizado entre os dois exercícios. Tanto o bíceps femoral (p = 0,041) quanto o glúteo máximo (p = 0,0059) apresentaram maior ativação no AF (30,9% para glúteo; 42,6% para bíceps) em comparação ao AP (21,2% para glúteo; 24,7% para bíceps). Apesar da ativação moderada durante a execução dos exercícios, girando em torno de 25% a 45%, a resposta foi maior, para os participantes do estudo, no AF.

The purpose of this study was to compare the EMG of the gluteus maximus and biceps femoris between the lunge and the parallel squat. Seven subjects, with experience in strength training, performed eight repetitions of the parallel squat (PS) and the lunge (LU) with an overload corresponding to 50% of body mass. The EMG of the gluteus maximus and biceps femoris was captured, filtered by a forth order Butterworth filter (20-400 Hz) and calculated RMS values. The Wilcoxon Ranked test was used to compare the normalized EMG of each muscle between the two exercises. Both the biceps femoris (p = 0.041) and the gluteus maximus (p = 0.0059) showed increased activation in LU compared to the PS. Despite the moderate activation in both exercises, ranging from 25% to 45%, the myoelectric response of the analyzed muscles was higher, for the participants, in the lunge exercise.

El objetivo de este estudio fue comparar la actividad mioeléctrica (EMG) del músculo glúteo mayor y el bíceps femoral entre la sentadilla con los pies uno frente al otro y los pies paralelos. Siete sujetos realizaron ocho repeticiones de la sentadilla paralela (AP) y con los pies uno frente al otro (FA) desplazando una masa igual a 50% del peso corporal. El EMG del músculo glúteo mayor y el bíceps femoral fueron capturados, filtrada por un filtro de banda de 4 º orden Butterworth (20-400 Hz) y los valores RMS obtenidos. Se utilizó el test de Wilcoxon para comparar la EMG normalizada entre los dos ejercicios. Tanto el bíceps femoral (p = 0,041) y el glúteo mayor (p = 0,0059) mostraron una mayor activación en la FA (30,9% para lo glúteo y 52% para lo bíceps) en comparación con el AP (21,2% para lo glúteo y 24,7% para lo bíceps). Aunque la activación moderada durante la ejecución de los ejercicios fue moderado, situándose en torno a un 25% a 45%, la respuesta fue mayor en el FA para los participantes del estudio.

In this study, 100 clinical isolates of Streptococcus agalactiae recovered from genitourinary tract specimens of non-pregnant individuals living in Rio de Janeiro were submitted for antimicrobial susceptibility testing, detection of macrolide resistance genes and evaluation of the genetic diversity of erythromycin-resistant isolates. By agar diffusion method, all isolates were susceptible to ceftazidime, penicillin and vancomycin. Isolates were resistant to levofloxacin (1%), clindamycin (5%), erythromycin (11%) and tetracycline (83%) and were intermediated to erythromycin (4%) and tetracycline (6%). Erythromycin-resistant and intermediated isolates presented the following phenotypes: M (n = 3), constitutive macrolide-lincosamide-streptogramin B (MLS B, n = 5) and inductive MLS B (n = 7). Determinants of macrolide resistance genes, erm and mef, were detected in isolates presenting MLS B and M phenotypes, respectively. Randomly amplified polymorphic DNA profiles of erythromycin-resistant isolates were clustered into two major groups of similarity.

OBJETIVO: Avaliar o uso a longo prazo do flavonóide quercetina em ratos cirróticos por ligadura de ducto biliar comum (LDB). MÉTODOS: Foram utilizados 32 ratos machos Wistar, sendo submetidos à LDB ou simulação, e distribuídos em 4 grupos: 1) controle, 2) cirróticos, 3) cirróticos tratados com quercetina 50mg/kg, intraperitonealmente, desde o segundo dia após o procedimento cirúrgico; e 4) cirróticos tratados após o décimo quarto dia do procedimento cirúrgico. Analisou-se a função hepática por meio de testes bioquímicos (BT e BD) e atividade enzimática (ALT, AST, FA e GGT). Na análise anatomopatológica, utilizou-se a coloração de Hematoxilina & Eosina (H&E) e de Picrosírius para fibrose. A análise estatística para avaliação de sobrevivência foi realizada pelo teste Kaplan-Meier. RESULTADOS: Os resultados de sobrevivência dos oito animais de cada grupo foram: Grupo 1 = 200 dias de sobrevivência; Grupo 2 = 46 dias; Grupo 3 = 71 dias; e o Grupo 4 = 90 dias. Nos animais com ligadura de ducto biliar comum houve aumento das provas de função hepática e enzimáticas que se reduziu hipoteticamente com o tratamento com quercetina. Foram identificadas cirrose, congestão vascular porta e centrolobular na análise histopatológica por H&E e Picrosírius. CONCLUSÃO: O uso da quercetina diminuiu de maneira significante as alterações bioquímicas provocadas pela cirrose, aumentando o tempo de sobrevivência dos animais com cirrose biliar secundária à LDB, como verificado pelo teste de análise de sobrevivência.

PURPOSE: The long term use of quercetin flavonoid was evaluated in cirrhotic rats by common biliary duct bondage (LDB). METHODS: 32 male Wistar rats were submitted to LDB or simulation, and distributed in 4 groups: 1) control, 2) cirrhotic, 3) cirrhotic treated with quercetin the second day after the surgical procedure; and 4) cirrhotic treated with quercetin after the fourteenth day of the surgical procedure. The hepatic function was analyzed through biochemical tests (TB and DB) and enzymatic activity (ALT, AST, AP and GGT). In the anatomopatological analysis, Hematoxilin & Eosin (H&E) coloration, and Picrosirius (for fibrosis) were used.The statistical analysis for survival evaluation was accomplished by the Kaplan-Meier test. RESULTS: The results of survival of the eight animals of each group were: Group 1 = 200 days of survival; Group 2 = 46 days; Group 3 = 71 days; and the Group 4 = 90 days. In the animals with bondage of common biliary duct, there was an increase of the hepatic and anzymatic function tests, which was reduced with the treatment with quercetin. Cirrhosis, portal and centrolobular vascular congestion, were identified in the histopatological analysis with H&E and Picrosirius. CONCLUSION: The use of quercetin decreased significantly the biochemical alterations caused by cirrhosis, increasing the time of survival of the animals with secondary biliary cirrhosis due to LDB.