Abstract The study aimed to characterize the digestive tract of Uranoscodon superciliosus and its associations to the diet and foraging behavior already described for the species. Five lizards were captured in forest areas near the city of Manaus, Amazonas, Brazil. Tongue, oesophagus, stomach, small and large intestines fragments were collected, fixed, and processed for light microscopy. Hyaline cartilage was present in the center of the tongue, and the papillae from the apex and glands from the radix showed positive reaction to Alcian blue. The oesophagus presented a folded mucosa, covered by an epithelium with mucous and goblet cells positive to PAS and Alcian blue. There was presence of gastric glands in the cardic and fundic stomach regions, plus all the regions reacted positively to PAS. Fold and villi variations in both small and large intestine were noted, as well as the number and arrangement of goblet cells. Mucous and goblet cells from the small intestine were positively stained in PAS, while only the goblet cells were Alcian blue positive. These findings indicate that the Amazonian Diving Lizard’s digestive tract organs, mainly the tongue and stomach, present morphologies associated to ambush-type foraging and a specific diet largely based on small invertebrates. species Manaus Amazonas Brazil Tongue collected fixed microscopy mucosa noted Lizards Lizard s organs ambushtype ambush type invertebrates

Abstract Based on the genetic, morphological, and morphometric data of blood gamonts, we identified Hepatozoon parasites in colubrid snakes sampled in the Eastern Amazon region. Hepatozoon trigeminum was detected in the mussurana snake Clelia clelia and exhibited wide and elongated gamonts (mean dimensions: 14.25±0.65 × 4.31±0.43 μm) with an evident parasitophorous vacuole. Hepatozoon odwyerae sp. nov. was described in the indigo snake Drymarchon corais, whose gamonts have elongated and thin bodies (mean dimensions: 13.41±0.79 × 3.72±0.35 μm) with one end more tapered than the other. Phylogenetic analyses, based on the amplification of a 441 bp fragment of the 18S rRNA gene, revealed that the novel sequences of Hepatozoon spp. from our study were closely related to hemogregarine lineages found in lizards and snakes from Brazil, forming a well-supported monophyletic clade with them. The present study provides the first species description of Hepatoozon in D. corais and a new record of a host species for C. clelia using the integrated taxonomic data. We also highlight the importance of further investigations into the diversity of Hepatozoon in snakes, a rich but underestimated group of parasites, especially in the Amazonian biome. genetic morphological region mean dimensions 1425065 14 25 0 65 14.25±0.6 431043 4 31 43 4.31±0.4 μm vacuole sp nov 1341079 13 41 79 13.41±0.7 372035 3 72 35 3.72±0.3 other analyses 44 S gene spp Brazil wellsupported well supported them D C biome 142506 1 2 6 14.25±0. 43104 4.31±0. 134107 7 13.41±0. 37203 3.72±0. 14250 14.25±0 4310 4.31±0 13410 13.41±0 3720 3.72±0 1425 14.25± 431 4.31± 1341 13.41± 372 3.72± 142 14.25 4.31 134 13.41 37 3.72 14.2 4.3 13.4 3.7 14. 4. 13. 3.

RESUMEN Objetivo: desarrollar una aplicación de atención primaria de enfermería para usuarios con diabetes mellitus y evaluar la usabilidad a la luz de la Teoría Unificada de Aceptación y Uso de la Tecnología. Materiales y métodos: estudio metodológico, que utilizó un enfoque cuantitativo y transversal, basado en el método de diseño instruccional sistemático. El producto fue validado para la usabilidad por 11 enfermeros especializadas, mediante el cuestionario de escala de usabilidad del sistema, basado en la Teoría Unificada de Aceptación y Uso de la Tecnología 2, de Venkatesh, Thong y Xu. Resultados: la aplicación se desarrolló por medio de frameworks abiertos y gratuitos, basados en el contenido actualizado del Protocolo de Enfermería en Atención Primaria sobre Diabetes Mellitus, más la Clasificación Internacional de Prácticas de Enfermería en Salud Pública. Al evaluar la usabilidad, la aplicación alcanzó un puntaje de 90 puntos, clasificándose en una escala de la mejor calidad posible, con dimensiones que sustentan las construcciones teóricas. Conclusiones: la tecnología llamada “Diabetes em foco” es prometedora para mejorar y avanzar en el conocimiento teórico y práctico, además del acceso rápido y fácil al protocolo, el apoyo oportuno para la toma de decisiones y el fortalecimiento de la sistematización de la atención de enfermería en atención primaria a la salud.

ABSTRACT Objective: To develop an application on primary nursing care for users with diabetes mellitus and to assess its usability in the light of the Unified Theory of Acceptance and Use of Technology. Materials and methods: A methodological study, which used a quantitative and cross-sectional approach, based on the Systematic Design of Instruction method. The product was validated for usability by 11 specialist nurses, using the System Usability Scale questionnaire, based on the Unified Theory of Acceptance and Use of Technology 2 by Venkatesh, Thong and Xu. Results: The application was developed through open and free frameworks, based on the updated content of the Nursing Protocol in Primary Care on Diabetes Mellitus, plus the International Classification of Nursing Practices in Public Health. In assessing usability, the application reached a score of 90 points, being classified on a scale of best achievable, with dimensions that ground the theoretical constructs. Conclusions: The technology named Diabetes em Foco (Diabetes in focus in English) is promising to improve and advance theoretical and practical knowledge, in addition to quick and easy access to the protocol, timely support for decision-making and strengthening the systematization of nursing care in primary health care.

RESUMO Objetivo: desenvolver um aplicativo sobre cuidados primários de enfermagem aos usuários com diabetes mellitus e avaliar sua usabilidade à luz da Teoria Unificada de Aceitação e Uso de Tecnologia. Materiais e métodos: trata-se de um estudo metodológico, que utilizou uma abordagem quantitativa e de delineamento transversal, a partir do método Design Instrucional Sistemático. O produto foi validado quanto à usabilidade por 11 enfermeiros especialistas, por meio do questionário System Usability Scale, fundamentado pela Teoria Unificada de Aceitação e Uso de Tecnologia 2 de Venkatesh, Thong e Xu. Resultados: o aplicativo foi desenvolvido por meio de frameworks abertos e sem custo, baseado no conteúdo atualizado do Protocolo de Enfermagem na Atenção Primária sobre Diabetes Mellitus, acrescido da Classificação Internacional das Práticas de Enfermagem na Saúde Coletiva. Na avaliação da usabilidade, o aplicativo alcançou um score de 90 pontos, sendo classificado numa escala de melhor alcançável, com dimensões que alicerçam os constructos teóricos. Conclusões: a tecnologia denominada “Diabetes em foco” apresenta-se promissora para melhorar e avançar o conhecimento teórico-prático, além do acesso rápido e facilitado ao protocolo, do apoio oportuno à tomada de decisões e do fortalecimento da sistematização da assistência de enfermagem na atenção primária à saúde.

Setenta e cinco tartarugas Podocnemis expansa foram examinadas para a presença de ectoparasitos e hemoparasitos na região amazônica brasileira. As coletas foram realizadas no Estado do Tocantins, Brasil. No estudo foram amostrados 25 indivíduos por local, um criatório comercial, um criatório de subsistência indígena e uma população silvestre do rio Javaés. Foram encontrados hemoparasitos pertencentes ao gênero Haemogregarina em 66% (50/75) dos animais, sendo que as infecções foram restritas ao criatório comercial e a população silvestre do rio Javaés. A parasitemia média foi de 54/2.000 eritrócitos (2%). Não houve relação entre o índice de condição corporal dos quelônios e os níveis de parasitemia e nem diferença quanto ao sexo dos animais. Não foram observadas sanguessugas durante os exames físicos, porém os animais do criatório comercial apresentaram condições físicas ruins com deformidades no casco e presença de uma quantidade relativamente alta de ulcerações na pele, provavelmente causadas por fungos ou bactérias. Este estudo registrou a primeira ocorrência de hemogregarinas em uma escala populacional de P. expansa e contribui para expandir o conhecimento de hemoparasitos em quelônios no Brasil.

Seventy-five turtles Podocnemis expansa in the Brazilian Amazon were examined for the presence of ectoparasites and hemoparasites. Samplings were performed in three study areas in the state of Tocantins, Brazil. Twenty-five specimens were sampled per study area (a commercial breeding facility, an indigenous subsistence breeding facility and a wild population of the Javaés River). Hemoparasites of the genus Haemogregarina were found in 66% (50/75) of the turtle specimens, and the infections were restricted to the commercial breeding facility and to the wild population of the Javaés River. The mean level of parasitemia was 54/2,000 erythrocytes (2%). There was no correlation between the body condition index of the chelonians and the level of parasitemia, with no significant difference between genders. No leeches were observed during the physical exams in any of the study areas, but the specimens from the commercial breeding facility were in poor physical condition with shell deformities and the presence of a relatively high amount of skin ulcerations, most likely caused by fungi and bacteria. This was the first study to record the occurrence of hemogregarines on a population scale in P. expansa and helps to increase knowledge about hemoparasites in chelonians in Brazil.