Abstract This study evaluated the median lethal concentration of silver nanoparticles and their effects in fish tambaqui Colossoma macropomum. Therefore, an acute toxicity assay was carried out in completely randomized design evaluating six different concentrations of silver nanoparticles on blood parameters of tambaqui. The silver nanoparticles were produced by chemical reduction with polyvinyl alcohol (AgNP-PVA). The lethal concentration 50% (LC50) was estimated using probit regression. The blood was collected, analyzed and the data were submitted to T-test (dying x surviving fish) and Tukey test (surviving fish). An increase in glucose, hematocrit, total plasma protein, hemoglobin, erythrocytes, leukocytes, monocytes, and neutrophils as well as reduced MCV (mean corpuscular volume) in dying fish compared to surviving fish were observed. Survived fish exposed to 187.5 µg/L showed an increase in hematocrit, MCV, and MCH and a reduction in erythrocytes, total numbers of leukocyte, thrombocyte, lymphocyte, and neutrophil. The fish exposed to concentrations below 125 µg/L, had returned the blood parameter to baselines compared to control. The estimated LC50 was 165.09 µg/L and was classified as highly toxic for the fish tambaqui. In higher concentrations, it causes an acute respiratory toxicity, but in concentrations below 125 µg/L, the fish can adapt to the stressing agent. macropomum Therefore AgNPPVA. AgNPPVA AgNP PVA . (AgNP-PVA) 50 LC (LC50 regression collected Ttest T fish. glucose hematocrit protein hemoglobin erythrocytes leukocytes monocytes mean volume observed 1875 187 5 187. µgL µg L leukocyte thrombocyte lymphocyte neutrophil 12 control LC5 16509 165 09 165.0 agent (AgNP-PVA (LC5 18 1 1650 16 0 165. (LC

Abstract The canned Amazon River prawn with sauce (tucupi and jambu) was thermally prepared (121 ºC) at three different times (T1: 15 T2: 20 and T3: 25 minutes) in glass bottles. The product obtained 20.86% crude protein, with good acceptance by consumers (global acceptance >7) measured through centesimal and sensory analysis of the hedonic scale. Increased exposure caused obvious changes in the sensorial characteristics of the product, which were not evidenced by consumers in the sensorial analysis, and for this reason, texture profile analysis (TPA) and color (CIELAB) were carried out, in addition to analyses of concentration of Total Volatile Basic Nitrogen (TVB-N), hydrogen potential (pH) and microbiological performed during 12 months, in addition to yield and weight loss by cooking. The sensory analysis did not show a significant difference between the treatments, however, the yield reduced with the application of heat, with the best yield being in treatment T1 (58.76 ± 0.49 a). The centesimal analysis showed excellent values of protein content of 20.86% and lipid content of 0.33%. The treatments followed the variation in color and texture according to the application of heat, mainly concerning the increase in the b* value (10.84 ± 0.70 to 13.50±0.24) and reduction in cohesiveness (0.56 ± 0.05 to 0.50 ± 0.04), gumminess (368.81± 109.23 to 262.09 ± 63.78 g) and chewiness (999.30 ± 356.92 to 633.71 ± 221.56 g). Despite this, the product proved to be safe during the analyzed period without the development of the remaining bacteria, even with an increase in pH (4.2 to 5.9) and TVB-N (20.22 to 28.46 mg/100 g), despite this, there was no development of the bacteria analyzed, proving to be a product suitable for industrial implementation. tucupi jambu 121 (12 ºC T (T1 1 T2 2 T3 minutes bottles 2086 86 20.86 global >7 7 scale reason TPA (TPA CIELAB (CIELAB out TVBN, TVBN TVB N , (TVB-N) (pH months cooking however heat 58.76 5876 58 76 (58.7 049 0 49 0.4 a. . a) 033 33 0.33% b 10.84 1084 10 84 (10.8 070 70 0.7 13.50±0.24 1350024 13 50 24 0.56 056 56 (0.5 005 05 0.0 050 0.5 0.04, 004 0.04 04 0.04) 368.81± 36881 368 81 (368.81 10923 109 23 109.2 26209 262 09 262.0 6378 63 78 63.7 g 999.30 99930 999 30 (999.3 35692 356 92 356.9 63371 633 71 633.7 22156 221 221.5 g. 4.2 42 4 (4. 5.9 59 5 9 20.22 2022 22 (20.2 2846 28 46 28.4 mg100 mg 100 mg/10 g, implementation (1 (T 208 8 20.8 > (TVB-N 58.7 587 (58. 0. 03 3 0.33 10.8 108 (10. 07 13.50±0.2 135002 (0. 00 368.81 3688 36 (368.8 1092 109. 2620 26 262. 637 6 63. 999.3 9993 99 (999. 3569 35 356. 6337 633. 2215 221. 4. (4 5. 20.2 202 (20. 284 28. mg10 mg/1 ( 20. 58. (58 0.3 10. (10 13.50±0. 13500 (0 368.8 (368. 999. (999 (20 mg1 mg/ (5 13.50±0 1350 368. (368 (99 (2 13.50± 135 (36 (9 13.50 (3 13.5 13.