RESUMO No México, o milho crioulo apresenta problemas de podridão das sementes. Portanto, o objetivo desta pesquisa foi determinar a frequência de fungos associados a sementes de milho crioulo. De janeiro e fevereiro de 2019, 13 amostras de milho crioulo foram coletadas em localidades de Guerrero, México. As amostras foram processadas em meio de cultura BDA e a frequência de cada fungo foi estimada com base nas características culturais e chaves taxonômicas. Nas amostras sintomáticas de sementes de milho, Fusarium oxysporum foi registrado em oito amostras com 97,2%, seguido por F. graminearum isolado na raça de milho “Olotillo 1” com 94,4% e na raça “Cónico pepitilla” com 58,3%. Phomopsis sp., foi isolado 100% na raça “Olotillo 2”. Gibberella moniliformis, foran isolados de “Elotes Occidental 1” com 83,3% e o menos frequente (19,4%) em “Pepitilla” foi Sarocladium strictum registrado em três amostras com valores baixos de 11,1% e 2,8% na raça de milho “Arrocillo 2” e “Pepitilla”, Talaromyces variabilis em duas amostras com 11,1% e 2,8% em “Arrocillo 2” e “Cónico pepitilla”. Stenocarpella macrospora foi isolado em 72,2% da raça de milho “Pepitilla”. Aspergillus flavus em “Cónico Pepitilla” com 16,7%, Setosphaeria turcica e Emericella sp., foi isolado com menor frequência. Em sementes assintomáticas da raça “Cónico pepitilla” foi isolado F. oxysporum com 8,3%, Fusarium acuminatum 8,3%, Gibberella moniliformis em 41,7%, em sementes assintomáticas de “Olotillo 1” e “Olotillo 2” Phomopsis sp., foi isolado em 50% e Rhizoctonia solani com 50%. Neste estudo, os fungos F. oxysporum, Phomopsis sp., G. moniliformis e F. graminearum foram os mais frequentemente associados às sementes de milho crioulo. México Portanto 2019 1 Guerrero taxonômicas 972 97 2 97,2% F Olotillo 944 94 4 94,4 Cónico pepitilla 583 58 3 58,3% sp sp. 100 2. . Elotes 833 83 83,3 19,4% 194 19 (19,4% Pepitilla “Pepitilla 111 11 11,1 28 8 2,8 Arrocillo Pepitilla, , pepitilla. 722 72 72,2 Pepitilla. 167 16 7 16,7% 8,3% 417 41 41,7% 50 estudo G 201 9 97,2 94, 5 58,3 10 83, 19,4 (19,4 11, 2, 72, 16,7 8,3 41,7 20 97, 58, 19, (19, 16, 8, 41, (19 (1 (
ABSTRACT In Mexico, Creole corn has presented problems related to seed rot. Therefore, the objective of this study was to determine the frequency of fungi associated with Creole corn seeds. From January to February 2019, 13 Creole corn samples were collected from several localities in Guerrero, Mexico. The samples were processed in PDA culture medium and the frequency of each fungus was estimated based on cultural characteristics and taxonomic keys. Considering symptomatic corn seed samples, Fusarium oxysporum was recorded in eight samples at 97.2%, followed by F. graminearum, isolated from race “Olotillo 1” at 94.4% and from race “Cónico pepitilla” at 58.3%. Phomopsis sp. was isolated at 100% from race “Olotillo 2”. Gibberella moniliformis was isolated from “Elotes Occidental 1” at 83.3%, while the least frequent isolate (19.4%) from “Pepitilla” was Sarocladium strictum, which was recorded in three samples at the low values of 11.1% and 2.8% for races “Arrocillo 2” and “Pepitilla”, respectively; Talaromyces variabilis was found in two samples at 11.1% and 2.8% from “Arrocillo 2” and “Cónico pepitilla”, respectively; Stenocarpella macrospora was isolated at 72.2% from “Pepitilla”. Aspergillus flavus was obtained from “Cónico Pepitilla” at 16.7%, while Setosphaeria turcica and Emericella sp. were less frequently isolated. Considering asymptomatic seeds of race “Cónico pepitilla”, F. oxysporum was isolated at 8.3%, Fusarium acuminatum at 8.3% and Gibberella moniliformis at 41.7%; from asymptomatic seeds of “Olotillo 1” and “Olotillo 2”, Phomopsis sp. was isolated at 50% and Rhizoctonia solani at 50%, respectively. In the present study, the fungi F. oxysporum, Phomopsis sp., G. moniliformis and F. graminearum were most frequently associated with Creole corn seeds. Mexico rot Therefore 2019 1 Guerrero keys 972 97 2 97.2% F Olotillo 944 94 4 94.4 Cónico pepitilla 583 58 3 58.3% sp 100 2. . Elotes 833 83 83.3% 19.4% 194 19 (19.4% Pepitilla “Pepitilla strictum 111 11 11.1 28 8 2.8 Arrocillo Pepitilla, , respectively pepitilla, 722 72 72.2 Pepitilla. 167 16 7 16.7% 8.3 41.7% 417 41 2, 50 G 201 9 97.2 94. 5 58.3 10 83.3 19.4 (19.4 11. 72. 16.7 8. 41.7 20 97. 58. 83. 19. (19. 16. 41. (19 (1 (