Parthenolide (PTN) is a secondary metabolite of the plant Tanacetum parthenium (L.) Schulz Bip. and is considered the chemical marker of this species. This sesquiterpene lactone (germacrene skeleton) has been described as responsible for the biological activity of the leaf extract. In addition, several studies in the literature have demonstrated its antiparasitic and antineoplastic activity. However, there is a need for knowledge of other safety parameters, such as pharmacokinetic, pharmacodynamic and toxicity evaluations. Therefore, in this investigation, the in vitro metabolism of parthenolide was performed with rat liver microsomes and biomimetic metabolism (such as cytochrome P-450 system) using organometallic catalysts. The biomimetic procedure was validated since the major compound of biomimetic oxidative reaction with meta-chloroperbenzoic acid catalyzed by metalloporphyrin 5,10,15,20-tetrakis(pentafluorophenyl)-porphyrin iron(III) chloride was also observed with rat liver microsomes. Previous chemoenzymatic synthesis studies of PTN afforded the same epoxide formation, this major oxidative compound was isolated and fully characterized as (1 R,10R)-epoxyparthenolide based on experimental and theoretical calculations of infrared (IR) and vibrational circular dichroism (VCD) data at the B3PW91/6-311G. Furthermore, for the first time, this metabolite was evaluated for trypanocidal and leishmanicidal activity. The mean inhibitory concentration (IC50) values were lower for PTN than its metabolite and showed significant cytotoxic effects for both parasites. This finding holds promise for addressing neglected diseases. (PTN L. L (L. Bip species germacrene skeleton extract addition However parameters pharmacokinetic evaluations Therefore investigation P450 P 450 P-45 system catalysts metachloroperbenzoic meta chloroperbenzoic 5,10,15,20tetrakispentafluorophenylporphyrin 5101520tetrakispentafluorophenylporphyrin tetrakispentafluorophenylporphyrin 5,10,15,20 tetrakis pentafluorophenyl porphyrin 5 10 15 20 ironIII iron III iron(III formation 1 ( R,10Repoxyparthenolide R10Repoxyparthenolide RRepoxyparthenolide R,10R epoxyparthenolide R 10R IR (IR VCD (VCD B3PW91/6311G. B3PW916311G BPWG B3PW91/6 311G. B3PW91 6 311G B PW G B3PW91/6-311G Furthermore time IC50 IC (IC50 parasites diseases (L P45 45 P-4 20tetrakispentafluorophenylporphyrin 5101520 5,10,15,2 2 10Repoxyparthenolide Repoxyparthenolide R10R RR 6311G B3PW91/6311G B3PW916 BPW B3PW91/ B3PW9 IC5 (IC5 P4 4 P- 510152 5,10,15, B3PW (IC 51015 5,10,15 5101 5,10,1 510 5,10, 51 5,10 5,1 5,

The essential oil extracted from Anemia tomentosa (EOAT) has shown larvicidal potential against Aedes aegypti, based on methods in vitro and in silico. Chromatographic and spectrometric techniques (gas chromatography-mass spectrometry (CG-MS), gas chromatography-flame ionization detector (GC-FID)), as well as mono and bidimensional nuclear magnetic resonance spectroscopy (NMR) were used to assess 10 essential oil components. Chemical composition of essential oil comprised 87.51% sesquiterpenes, with emphasis on presilphiperfolan-1-ol (42.13%) and silphiperfol-5-ene (19.47%). Larvicidal assay conducted in vitro with EOAT has evidenced potential cytotoxic activity up to 48 h exposure to it; mortality rate observed for A. aegypti larvae exposed to essential oil reached 100%. Study conducted in silico with chemical compounds deriving from the herein investigated plant species has evidenced its potential to inhibit acetylcholinesterase in A. aegypti. Activity of triquinane sesquiterpenes ranging from -6.8 to -8.2 kcal mol-1 stood out in comparison to that of temephos (-7.5 kcal mol-1). Chemical compounds identified in the investigated essential oil presented low human and environmental toxicity, as observed in absorption, distribution, metabolism and excretion, and toxicity (ADMETox) predictions. (EOAT chromatographymass chromatography mass CGMS, CGMS CG MS , (CG-MS) chromatographyflame flame GCFID, GCFID GC FID (GC-FID)) NMR (NMR 1 components 8751 87 51 87.51 presilphiperfolan1ol presilphiperfolanol presilphiperfolan ol 42.13% 4213 42 13 (42.13% silphiperfol5ene silphiperfolene silphiperfol 5 ene 19.47%. 1947 19.47% . 19 47 (19.47%) 4 it A 100 100% 6.8 68 6 8 -6. 8.2 82 2 -8. mol1 mol mol- 7.5 75 7 (-7. mol1. mol-1) absorption distribution excretion ADMETox (ADMETox predictions (CG-MS (GC-FID) 875 87.5 42.13 421 (42.13 194 19.47 (19.47% 6. -6 8. -8 7. (-7 (GC-FID 87. 42.1 (42.1 19.4 (19.47 - (- 42. (42. 19. (19.4 ( (42 (19. (4 (19 (1

Species from Pavonia genus (Malvaceae) are traditionally used as antioxidant, n anti inflammatory and antimicrobial remedies. Previous studies have revealed their diversity of specialized metabolites widely used in the development of new medicines. This is the first study on Pavonia glazioviana Gurke, a Brazilian species known as “Malva da Chapada”. The research led to identify fifteen compounds: 132 (S) hydroxy pheophytin a; and 132(S) hydroxy 173 ethoxyphaeophorbide, n decanol, cycloart 23Z ene 3β,25 diol, cycloart 24S 25 ene 3β,24 diol, sitosterol 3 O β D glucopyranoside, stigmasterol 3 O β D glucopyranoside, quercetin, acacetin, kaempferol, tiliroside, 5 hydroxy 3,7,8,4’ tetramethoxyflavone, 5,7 dihydroxy 3,8,4’ trimethoxyflavone, 5,7 dihydroxy 4’ methoxyflavone, 5,7,4’ trihydroxy 3,8 dimethoxyflavone and 5,7,4’ trihydroxy 3 methoxyflavone. The major flavonoids were tested for their antimicrobial activity, and the compound 5,7 dihydroxy 3,8,4’ trimethoxyflavone showed strong activity against Escherichia coli (MIC = 512 μg mL1), Pseudomonas aeruginosa (MIC = 512 μg mL1), Candida albicans (MIC = 512 μg mL1), Candida tropicalis (MIC = 512 μg mL1), Candida parapsilosis (MIC = 512 μg mL1), Aspergillus flavus (MIC = 512 μg mL1) and A. fumigantus (MIC = 512 μg mL1). The total phenolic content, flavonoid content, and radical scavenging potential were determined showing its great production of phenolics and flavonoids, along with interesting radical scavenging potential (DPPH EC50 = 6.36 ± 0.029 mg mL 1). Our findings contributed to characterize P. glazioviana as a source of methoxylated flavonoids with antimicrobial potential. Malvaceae (Malvaceae antioxidant remedies medicines Gurke Malva Chapada. Chapada . Chapada” compounds 13 S (S 132S 132(S 17 ethoxyphaeophorbide decanol Z 3β25 3β 3β,2 diol 2 3β24 24 glucopyranoside quercetin acacetin kaempferol tiliroside 3,7,8,4 3784 7 8 4 tetramethoxyflavone 57 5, 3,8,4 384 methoxyflavone 5,7,4 574 38 3, MIC 51 mL1, mL1 , A mL1. content DPPH EC EC5 636 6 36 6.3 0029 0 029 0.02 1. 1 1) P 3β2 3β, 3,7,8, 378 3,8, 5,7, 63 6. 002 02 0.0 3,7,8 37 00 0. 3,7, 3,7

The history of the discovery, use, and development of dyes is closely linked to the history of humanity itself. Among the first records of human civilization are cave paintings made from, e.g., fruits, plants, minerals, and insects. Over the centuries, using different techniques, colors have been given to all types of materials and products, from clothing to food. The chemicals responsible for colors are dyes - a vast collection of molecules of the most varied compositions, but which have in common the absorption of light within the visible window of the electromagnetic spectrum. This article aims not only to review the properties of some classes of natural dyes, their origins, and their applications but also to describe the various computational chemistry methods involved in designing new bio-inspired molecules. discovery use itself eg e g e.g. fruits plants minerals insects centuries techniques products food compositions spectrum origins bioinspired bio inspired e.g

RESUMO Regiões com climas semiáridos apresentam condições ambientais que resultam em estresses abióticos às plantas. A maior área de produção de melão (Cucumis melo L.) do Brasil, o Estado do Rio Grande do Norte, possui essas características climáticas. O uso de bioestimulantes nesses sistemas de produção pode mitigar os efeitos dos estresses abióticos e aumentar a produtividade e a qualidade dos frutos. O objetivo neste estudo foi avaliar as características fisiológicas e de produção do meloeiro, sob aplicação de bioestimulantes. Os bioestimulantes Acadian®, Folicist® e Nov@® foram aplicados e comparados ao tratamento controle (sem bioestimulantes), em melões ‘Goldex’ e ‘McLaren’. Nov@® proporcionou maior assimilação de CO2, transpiração e condutância estomática, assim como ascorbato peroxidase em ‘Goldex’ e ‘McLaren’. Maior catalase foi obtida pela aplicação de Nov@®, enquanto que o uso de Folicist® resultou em maior teor de malondialdeído. A cultivar McLaren apresentou o melhor desempenho fisiológico e bioquímico e a ‘Goldex’ o melhor rendimento. Folicist® promove adaptações fisiológicas aos estresses sem prejudicar a atividade fotossintética. Os bioestimulantes aumentaram a produtividade e o número de frutos por planta e promoveram adaptações fisiológicas às condições semiáridas. plantas Cucumis L. L Brasil Norte climáticas meloeiro Acadian Acadian® Folicist Nov Nov@ bioestimulantes, , bioestimulantes) ‘Goldex Goldex ‘McLaren. ‘McLaren . ‘McLaren’ CO2 CO estomática malondialdeído rendimento fotossintética semiáridas

ABSTRACT Regions with semi-arid climates have environmental conditions that result in abiotic stress to plants. The largest melon (Cucumis melo L.) production area in Brazil, the state of Rio Grande do Norte, has these climatic characteristics. Use of biostimulants in these production systems can mitigate effects of abiotic stress and increase yield and fruit quality. The objective in this study was to evaluate the physiological and production characteristics of melon, under the application of biostimulants. The biostimulants Acadian®, Folicist® and Nov@® were applied and compared to the control treatment (no biostimulants), in ‘Goldex’ and ‘McLaren’ melons. Nov@® led to higher CO2 assimilation, transpiration and stomatal conductance, as well as ascorbate peroxidase, in ‘Goldex’ and ‘McLaren’. Greater catalase was obtained with the application of Nov@®, while the use of Folicist® resulted in higher malondialdehyde content. The cultivar ‘McLaren’ exhibited the best physiological and biochemical performance, and ‘Goldex’ the best yield. Folicist® promotes physiological adaptations to stress without impairing photosynthetic activity. The biostimulants increased yield and number of fruits per plant and promoted physiological adaptations to semi-arid conditions. semiarid semi arid plants Cucumis L. L Brazil Norte quality Acadian Acadian® Folicist Nov Nov@ no biostimulants, , biostimulants) ‘Goldex Goldex ‘McLaren McLaren melons CO assimilation conductance peroxidase ‘McLaren. . content performance activity

Methods used to determine pesticide residues in teas typically require several laborious steps for sample preparation before analysis. In this study, a simple method based on solid-liquid extraction with low-temperature partitioning (SLE/LTP) and analysis by gas chromatography coupled to mass spectrometry (GC-MS) was optimized and validated for the determination of pesticide residues (pirimiphos-methyl, flutriafol, cyproconazole, and bifenthrin) in dehydrated green tea leaves. After low-temperature partitioning (LTP), the extract in acetonitrile was subjected to a clean-up step using primary secondary amine (PSA) and octadecylsilane (C18) as sorbents for chlorophyll removal. The optimized SLE/LTP-GC-MS method was validated, and it proved to be effective and selective for extracting pesticides from green tea samples, presenting limit of detection (LOD) and limit of quantification (LOQ) of 0.015 and 0.050 mg kg-1, respectively. Recovery ranged between 81-111%, coefficients of variation were less than 16% and coefficients of determination (R2) were greater than 0.990. The optimized and validated method was applied to green tea and 13 other tea varieties sampled in South and Southeast regions of Brazil. The pesticides under study were detected in some of those samples at values higher than the maximum residue limits (MRLs) allowed by the European Union. solidliquid solid liquid lowtemperature low temperature SLE/LTP SLELTP SLE LTP (SLE/LTP GCMS GC MS (GC-MS pirimiphosmethyl, pirimiphosmethyl pirimiphos methyl, methyl (pirimiphos-methyl flutriafol cyproconazole bifenthrin leaves LTP, , (LTP) cleanup clean up PSA (PSA C18 C (C18 removal SLE/LTPGCMS SLELTPGCMS LOD (LOD LOQ (LOQ 0015 0 015 0.01 0050 050 0.05 kg1, kg1 kg 1, 1 kg-1 respectively 81111%, 81111 81 111%, 111 81-111% 16 R2 R (R2 0990 990 0.990 Brazil MRLs (MRLs Union (LTP C1 (C1 LTPGCMS 001 01 0.0 005 05 kg- 81111% 8111 8 111% 11 81-111 (R 099 99 0.99 (C 00 0. 811 81-11 09 9 0.9 81-1 81-

This study details the preparation, antileishmanial assay, and in silico analysis of twenty 2-hydroxy-3-(1,2,3-triazolyl)propyl vanillin derivatives. These compounds were synthesized in three steps and evaluated against Leishmania infantum, Leishmania amazonensis, and Leishmania braziliensis promastigotes. Compounds 3s and 3t were the most effective, showing good activity against all Leishmania species tested. Molecular docking indicated that all compounds bind favorably to the sterol 14α-demethylase (CYP51) enzyme from L. infantum. ADMET (absorption, distribution, metabolism, excretion and toxicity) analysis indicated good oral bioavailability, non blood-brain barrier penetration, and high gastrointestinal absorption. Posaconazole and compounds 3e, 3s, and 3t remained stable in the CYP51 binding region during 100 ns molecular dynamics (MD) simulations. Root mean square deviation (RMSD) and root mean square fluctuations (RMSF) analyses from the MD trajectory revealed significant conformational fluctuations of the CYP51 N-terminal, suggesting occasional expulsion of 3e, potentially explaining its higher IC50 (half-maximal inhibitory concentration) values. Pairwise decomposition analyses from molecular mechanics Poisson-Boltzmann surface area (MM/PBSA) calculations highlighted the importance of hydrophobic residues in interacting with the synthesized derivatives. preparation assay 2hydroxy31,2,3triazolylpropyl 2hydroxy3123triazolylpropyl hydroxytriazolylpropyl 2 hydroxy 3 1,2,3 triazolyl propyl 1 derivatives infantum amazonensis promastigotes s t effective tested 14αdemethylase αdemethylase 14α demethylase α CYP (CYP51 L absorption, absorption (absorption distribution metabolism toxicity bioavailability bloodbrain blood brain penetration 3e e CYP5 10 (MD simulations RMSD (RMSD RMSF (RMSF Nterminal, Nterminal N terminal, terminal N-terminal IC IC5 halfmaximal half maximal concentration values PoissonBoltzmann Poisson Boltzmann MM/PBSA MMPBSA MM PBSA (MM/PBSA 2hydroxy31 3triazolylpropyl triazolylpropyl 123 1,2, (CYP5 2hydroxy3 12 1,2 (CYP 2hydroxy 1,

RESUMO Objetivou-se estudar a fenologia reprodutiva e o requerimento térmico de duas espécies de pitaia nas condições climáticas do extremo Norte da Amazônia. O experimento foi realizado no município de Boa Vista, Roraima durante dois ciclos produtivos (2020/2021 e 2021/2022). Utilizou-se duas espécies de pitaia, a Selenicereus costaricensis, com frutos de casca vermelha e polpa roxa e formato cilíndrico e a Hylocereus polyrhizus, com frutos de casca e polpa vermelha e formato alongado. Selecionou-se 10 plantas de cada espécie, dividindo-as em quatro quadrantes, sendo selecionado 10 cladódios por quadrante e 2 gemas floral por cladódio, totalizando 800 flores por espécie, as quais foram acompanhadas da intumescência da gema até a colheita dos frutos. Avaliou-se os períodos fenológicos da poda até a colheita dos frutos, baseando-se na descrição dos estádios fenológicos da escala Biologische Bundesanstalt Bundessortenamt and Chemical Industry (BBCH). As condições climáticas influenciaram as espécies entre os anos, principalmente devido as variações de temperatura do ar no momento da que antecede as fenofases. As variações entre os anos ocasionaram um aumento no ciclo produtivo de 20 dias para S. costaricensis e 34 dias para H. polyrhizus entre o primeiro (2020/2021) e segundo (2021/2022) ano produtivo. As propriedades organolépticas das espécies apresentaram excelentes resultados, como sólidos solúveis, acidez titulável e relações sólidos solúveis/ acidez titulável, inerente as espécies. A maturidade fisiológica completa de frutos de H. costaricensis e H. polyrhizus ocorre aos 31 e 38 dias após a antese (DAA), quando os frutos apresentam coloração vermelho intenso da casca e da polpa. Objetivouse Objetivou se Amazônia Vista 2020/2021 20202021 2020 2021 (2020/202 2021/2022. 20212022 2021/2022 . 2022 2021/2022) Utilizouse Utilizou alongado Selecionouse Selecionou 1 espécie dividindoas dividindo quadrantes cladódio 80 Avaliouse Avaliou baseandose baseando BBCH. BBCH (BBCH) fenofases S 3 H (2021/2022 resultados solúveis DAA, DAA , (DAA) 2020/202 2020202 202 (2020/20 2021202 2021/202 8 (BBCH (2021/202 (DAA 2020/20 202020 (2020/2 202120 2021/20 (2021/20 2020/2 20202 (2020/ 20212 2021/2 (2021/2 2020/ (2020 2021/ (2021/ (202 (2021 (20 (2 (

ABSTRACT The objective of this work was to assess the reproductive phenology and thermal requirements of two dragon fruit species grown under the climate conditions of northern Amazon. The experiment was carried out in the municipality of Boa Vista, Roraima, Brazil, during two production cycles (2020-2021 and 20212022). Two dragon fruit species were used: Hylocereus costaricensis, which produces cylindrical fruits with a red skin and purple pulp; and Hylocereus polyrhizus, which produces elongated fruits with red skin and pulp. Ten plants of each species were selected and divided into four quadrants; ten cladodes were selected per quadrant and two flower buds were selected per cladode, totaling 800 flowers for each species. The plants were monitored from the beginning of flower bud intumescence until fruit harvest. Phenological periods were evaluated from production pruning to fruit harvest, based on the phenological stage descriptions of the Biologische Bundesanstalt Bundessortenamt and Chemical Industry (BBCH) scale. Climate conditions affected the species over the years, mainly due to variations in air temperature preceding phenophases. Variations between years increased the production cycle in 20 days for H. costaricensis and 34 days for H. polyrhizus from the first (2020-2021) to the second (2021-2022) production year. Organoleptic properties of species showed excellent results, including soluble solids (SS), titratable acidity (TA), and SS to TA ratio. Complete physiological maturity of H. costaricensis and H. polyrhizus fruits occurred at 31 and 38 days after anthesis, respectively, when the fruits presented an intense red skin and pulp. Amazon Vista Roraima Brazil 20202021 2020 2021 (2020-202 20212022. 20212022 . 20212022) used pulp quadrants cladode 80 harvest BBCH (BBCH scale phenophases 2 H 3 2022 (2021-2022 year results SS, , (SS) TA, (TA) ratio anthesis respectively 2020202 202 (2020-20 2021202 8 (2021-202 (SS (TA 202020 (2020-2 202120 (2021-20 20202 (2020- 20212 (2021-2 (2020 (2021- (202 (2021 (20 (2 (

RESUMO O uso de estimulante de crescimento é uma tecnologia promissora para a agricultura por proporcionar melhor condição de crescimento para as plantas, pois são oriundos da mistura de reguladores vegetais, minerais, aminoácidos e outras substâncias que têm sido testadas em diferentes plantas. Assim, objetivou-se com este estudo avaliar os efeitos do tratamento com micronutrientes e aminoácidos em sementes e adubação foliar em plantas de melancia (Citrullus lanatus), sobre o crescimento inicial das plantas. O delineamento experimental utilizado foi o de blocos casualizados, com quatro repetições e arranjo fatorial 6 x 2 composto por seis doses do tratamento de sementes (0; 1; 2; 3; 4 e 5 mL kg-1 sementes) e sem ou com adubação foliar utilizando o bioestimulante na dose de 300 mL ha-1. Foram avaliados: número de folhas por planta; comprimento da parte aérea, raiz e total das plantas (cm); massa seca da parte aérea, raiz e total das plantas. A dose de 4 mL kg-1 sementes de melancia, cultivar Crimson Sweet, no tratamento de sementes e a adubação foliar no vigésimo sexto dia após a emergência das plântulas na dose de 300 ml ha-1 proporcionam melhores condições de crescimento as plantas devido aos incrementos nas variáveis número de folhas, comprimento e massa seca da parte aérea e total. vegetais minerais Assim objetivouse objetivou se Citrullus lanatus, lanatus , lanatus) casualizados 0 (0 1 3 kg1 kg kg- 30 ha1. ha1 ha 1. avaliados planta cm (cm) Sweet ha- ( (cm

ABSTRACT Plant growth stimulants are a promising technology for agriculture to improve plant growth. These stimulants are often derived from a blend of plant regulators, minerals, amino acids, and other substances tested on various plants. The objective of this study was to evaluate the effects of a growth stimulant (micronutrients and amino acids) for seed treatment and foliar application on the initial growth of watermelon (Citrullus lanatus; cultivar Crimson Sweet). A randomized block experimental design with four replications was used, in a 6×2 factorial arrangement consisted of six growth stimulant rates (0, 1, 2, 3, 4, and 5 mL kg-1) for seed treatment, combined or not with foliar application of the same growth stimulant at 300 mL ha-1 26 days after sowing. The plant characteristics evaluated were: number of leaves per plant; shoot, root, and total plant lengths (cm); and shoot, root, and total dry weights. The use of the growth stimulant rate of 4 mL kg-1 for treating watermelon seeds, combined with foliar application of the growth stimulant (300 mL ha-1 26 days after sowing), provided the best growth conditions to the plants, which was shown by their higher number of leaves, shoot length, and shoot and total dry weights. regulators minerals acids plants micronutrients Citrullus lanatus Sweet. Sweet . Sweet) used 62 6 2 6× 0, 0 (0 1 3 kg1 kg 30 ha1 ha ha- sowing were root cm (cm) weights kg- seeds (30 sowing, , sowing) length ( (cm (3

RESUMO A aplicação de cinza vegetal na agricultura surge como técnica promissora para potencializar a produtividade agrícola. Sua composição química tem aptidão para aprimorar os atributos do solo, atuando como fertilizante, corretivo e auxiliando na retenção de água. Entretanto, estudos recomendam adoção controlada, para evitar impactos adversos. O objetivo da pesquisa foi avaliar o potencial da cinza vegetal como estratégia de adubação na cultura do amendoim, com ênfase na otimização da produtividade das plantas e na redução do déficit hídrico. O experimento foi conduzido em casa de vegetação em vasos de 5 dm3; o delineamento experimental aplicado foi em blocos casualizados, formando um esquema fatorial 5x5, correspondente a cinco doses de cinza vegetal (0; 8; 16; 24 e 32 g dm3) e cinco disponibilidades hídricas (25; 50; 75; 100 e 125% da capacidade de campo), com quatro repetições. A cultivar utilizada foi a IAC OL-3. Os dados foram submetidos ao teste de normalidade para verificar se seguiram a distribuição normal e, posteriormente, submetidos à análise de variância e, quando significativo, submetidos à análise de regressão, a 5% de probabilidade de erro. A cinza vegetal, rica em nutrientes demonstrou ser uma alternativa viável de fonte de nutrientes. Doses entre 18 e 23 g dm-3 de cinza vegetal apresentaram os resultados mais satisfatórios. A disponibilidade hídrica que melhor respondeu foi de 96 a 112%. A cinza vegetal, nas doses de 23 e 20 g dm-3, contribuiu no consumo da água e na eficiência hídrica do solo. agrícola solo fertilizante Entretanto controlada adversos amendoim hídrico dm3 dm casualizados 5x5 x 0 (0 8 16 2 3 25 (25 50 75 10 125 campo, campo , campo) repetições OL3. OL3 OL 3. OL-3 posteriormente significativo regressão erro 1 dm- satisfatórios 9 112 112% dm3, 3, 5x ( (2 7 12 OL- 11

ABSTRACT The application of wood ash in agriculture emerges as a promising technique to enhance agricultural productivity. Its chemical composition can improve soil attributes, acting as a fertilizer, corrective agent, and assisting in water retention. However, studies recommend controlled adoption to avoid adverse impacts. The objective of this article is to evaluate the potential of wood ash as a fertilization strategy in peanut cultivation, with an emphasis on optimizing plant productivity and reducing water deficit. The experiment was carried out in a greenhouse in 5 dm3 pots; the experimental design applied was randomized blocks, forming a 5x5 factorial scheme, corresponding to five doses of wood ash (0; 8; 16; 24 and 32 g dm-3) and five levels of water availability (25; 50; 75; 100 and 125% of field capacity), with four repetitions. The cultivar used was IAC OL-3. The data were subjected to the normality test to check whether they followed the normal distribution and, subsequently, subjected to analysis of variance and, when significant, subjected to regression analysis, at a 5% probability of error. Wood ash, rich in nutrients, proved to be a viable alternative source of nutrients. Doses between 18.4 and 22.8 g dm-3 of wood ash led to the most satisfactory results. The water availability that responded best ranged from 96 to 111.3%. Wood ash, at doses of 22.8 and 19.64 g dm-3, contributed to water consumption and soil water efficiency. attributes fertilizer agent retention However impacts cultivation deficit dm pots blocks x 5x scheme 0 (0 8 16 2 3 25 (25 50 75 10 125 capacity, capacity , capacity) repetitions OL3. OL3 OL 3. OL-3 subsequently significant error nutrients 184 18 4 18. 228 22 22. dm- results 9 1113 111 111.3% 1964 19 64 19.6 dm3, 3, efficiency ( 1 (2 7 12 OL- 11 111.3 196 6 19. 111.

RESUMO Compostos derivados de petróleo presentes no solo podem comprometer o crescimento das plantas e inibir o desenvolvimento da população microbiana. Esses contaminantes diminuem a porosidade do solo, dificultam a absorção de água e podem interromper processos metabólicos das plantas. Assim, nesta pesquisa buscou-se avaliar os efeitos da presença no solo de três hidrocarbonetos no solo sobre o crescimento e desenvolvimento de espécies forrageiras e nos indicadores microbiológicos do solo. O experimento foi realizado em casa de vegetação no delineamento experimental de blocos casualizados, com quatro repetições. Os tratamentos foram dispostos em fatorial de 5×4, sendo o primeiro fator constituído pelas espécies avaliadas (Pennisetum glaucum, Zea mays, Brachiaria ruziziensis, Panicum maximum e Sorghum bicolor), e o segundo fator correspondendo a presença dos contaminantes: benzeno, tolueno ou xileno e solo sem contaminante. As espécies foram cultivadas por 42 dias. A tolerância das espécies dependeu do tipo de contaminante presente no solo. A massa seca total da P. glaucum foi reduzida em 26%, 10% e 32% para o tolueno, benzeno e xileno, respectivamente. Z. mays teve o crescimento reduzido na presença do tolueno (13%) e do benzeno (21%). S. bicolor teve sua matéria seca aumentada em 58% com o xileno. B. ruziziensis e P. maximum foram tolerantes para todos os contaminantes. A magnitude dos efeitos dos contaminantes nos indicadores microbiológicos do solo dependeu da espécie cultivada. A tolerância das plantas e da comunidade microbiana do solo aos contaminantes depende da espécie forrageira utilizada e deve ser aspecto importante para a seleção de plantas para recuperação de áreas degradadas. Assim buscouse buscou se casualizados repetições 54 5 4 5×4 Pennisetum bicolor, , bicolor) dias P 26 26% 10 32 respectivamente Z 13% 13 (13% 21%. 21 21% . (21%) S 58 B cultivada degradadas 5× 2 1 3 (13 (21% (1 (21 ( (2

ABSTRACT Oil-derived compounds present in the soil can compromise plant growth and inhibit the development of the microbial population. These contaminants reduce soil porosity, hinder water absorption, and can disrupt plant metabolic processes. The aim of this research was to evaluate the effects of the presence of three hydrocarbons in the soil on the growth and development of forage species and on soil microbiological indicators. The experiment was carried out in a greenhouse using a randomized block design with four replications. Treatments were arranged in a 5×4 factorial, with the first factor consisting of the species evaluated (Pennisetum glaucum, Zea mays, Brachiaria ruziziensis, Panicum maximum and Sorghum bicolor) and the second factor corresponding to the presence of contaminants: benzene, toluene or xylene and soil without contaminants. The species were grown for 42 days. The tolerance of the species depended on the type of contaminant present in the soil. The total dry matter of P. glaucum was reduced by 26%, 10% and 32% for toluene, benzene and xylene, respectively. Z. mays had its growth reduced in the presence of toluene (13%) and benzene (21%). S. bicolor had its dry matter increased by 58% with xylene. B. ruziziensis and P. maximum were tolerant to all contaminants. The magnitude of the effects of contaminants on soil microbiological indicators depended on the species grown. The tolerance of plants and soil microbial community to contaminants depends on the forage species used and should be an important aspect when selecting plants to restore degraded areas. Oilderived Oil derived population porosity absorption processes replications 54 5 4 5× factorial Pennisetum days P 26 26% 10 32 respectively Z 13% 13 (13% 21%. 21 21% . (21%) S 58 B areas 2 1 3 (13 (21% (1 (21 ( (2

Abstract Introduction: Iron (Fe) and manganese (Mn) are bioessential micronutrients for plants but can impair the energetic metabolism when present at high levels. Objective: To assess the photosynthetic performance and oxidative damages in Rhizophora mangle L. leaf tissues at low and high concentrations of Fe (74 and 195 mg kg-1; Feleaf) and Mn (65 and 414 mg kg-1; Mnleaf). Methods: Photosynthetic pigments, chlorophyll a fluorescence, leaf CO2 assimilation and gas exchange and DPPH• radical scavenging capacity were sampled in R. mangle growing in estuarine forests in the North region of Espírito Santo State and the extreme South of Bahia State (Brazil) showing low and high Feleaf and Mnleaf. Results: Effects of high Fe and Mn were not identified on pigment levels. The increase in Feleaf and Mnleaf at the levels observed in this assessment had a positive effect on the number of reaction centers and on the efficiency of the oxygen-evolving complex, evaluated as K-band, while no changes were found in the parameters related to the excitation trapping efficiency at the active center of photosystem II. Distinct interference patterns of Fe and Mn on the functional processes of photosynthesis were identified, especially on CO2 assimilation and reactive oxygen species metabolism, with major effects on CO2 assimilation and carboxylation efficiency of Rubisco at high Mnleaf. Conclusion: These findings demonstrate the efficiency of R. mangle in positively regulating the electron transport chain in response to high Fe and Mn, at least in terms of the preservation of structure and functionality of the plant photosynthetic apparatus. Moreover, interference of high Mnleaf in R. mangle occurs at non-stomatal and biochemical levels. There is an antagonistic interference of these trace elements with the physiology of R. mangle, which is a dominant species in Brazilian mangroves.

Resumen Introducción: El hierro (Fe) y el manganeso (Mn) son micronutrientes bioesenciales para las plantas, pero pueden afectar el metabolismo energético cuando están presentes en niveles altos. Objetivo: Evaluar el desempeño fotosintético y los daños oxidativos en tejidos foliares de Rhizophora mangle L. a bajas y altas concentraciones de Fe (74 y 195 mg kg-1; Feleaf) y Mn (65 y 414 mg kg-1; Mnleaf). Métodos: Se muestrearon pigmentos fotosintéticos, fluorescencia de clorofila a, asimilación e intercambio de gases de CO2 foliar y capacidad de eliminación de radicales DPPH• en R. mangle que crece en bosques de estuarios en la región Norte del estado de Espírito Santo y el extremo Sur del Estado de Bahía (Brasil) mostrando Feleaf y Mnleaf bajos y altos. Resultados: No se identificaron efectos de niveles elevados de Fe y Mn en los niveles de pigmento. El aumento de Feleaf y Mnleaf en los niveles observados en esta evaluación tuvo un efecto positivo en el número de centros de reacción y en la eficiencia del complejo generador de oxígeno, evaluado como banda K, mientras que no se encontraron cambios en los parámetros relacionados con la eficiencia de atrapamiento de excitación en el centro activo del fotosistema II. Se identificaron distintos patrones de interferencia de Fe y Mn en los procesos funcionales de la fotosíntesis, especialmente en la asimilación de CO2 y el metabolismo de las especies reactivas de oxígeno, con efectos importantes en la asimilación de CO2 y la eficiencia de carboxilación de Rubisco a niveles altos de Mnleaf. Conclusión: Los hallazgos demuestran la eficiencia de R. mangle en la regulación positiva de la cadena de transporte de electrones en respuesta a los altos niveles de Fe y Mn, al menos en términos de preservación de la estructura y funcionalidad del aparato fotosintético de la planta. Además, la interferencia de Mnleaf alto en R. mangle se presenta a niveles no estomáticos y bioquímicos. Hay interferencia antagónica de estos oligoelementos con la fisiología de R. mangle, que es una especie dominante en los manglares brasileños.

Resumo Introdução: O exercício físico está sendo incorporado ao tratamento de pacientes em hemodiálise, porém pouco se sabe sobre as principais características dessas intervenções. Objetivo: Descrever os protocolos de exercício físico prescritos para pacientes em hemodiálise no Brasil. Métodos: Uma revisão de escopo foi conduzida de acordo com as diretrizes JBI e Prisma-ScR. Foram realizadas pesquisas na Medline, Embase e em outras três bases de dados até maio de 2024. Outras fontes (sites, livros e diretrizes) também foram pesquisadas. Foram incluídas evidências de pacientes em hemodiálise, descrevendo protocolos de exercício físico em todos os ambientes e desenhos no Brasil. Resultados: Encontradas 45 evidências, resultando em 54 protocolos de exercício físico de 16 estados brasileiros. O exercício de força (33,3%), seguido do exercício aeróbico (22,2%), foi o mais prescrito para ser realizado durante a diálise (85,2%). Os profissionais mais prevalentes na supervisão dos programas foram fisioterapeutas e profissionais de educação física (37,0% e 18,5%, respectivamente). Todos os protocolos adotaram os princípios de treinamento tipo e frequência, enquanto a progressão foi adotada em apenas 53,7%. A frequência mais prescrita foi três vezes por semana (88,9%). A intensidade do exercício foi determinada predominantemente por métodos subjetivos (33,3%). Conclusão: Os exercícios aeróbicos e de força durante a diálise foram as modalidades mais prescritas no Brasil, com a maioria dos programas sendo adequadamente supervisionada por profissionais qualificados. No entanto, os protocolos existentes não adotaram a progressão sistemática no decorrer da intervenção, o que seria adequado para proporcionar melhores respostas e adaptações fisiológicas. Introdução intervenções Objetivo Brasil Métodos PrismaScR. PrismaScR Prisma ScR. ScR Prisma-ScR Medline 2024 sites, sites (sites pesquisadas Resultados 4 5 1 brasileiros 33,3%, 333 33,3% , 33 3 (33,3%) 22,2%, 222 22,2% 22 2 (22,2%) 85,2%. 852 85,2% . 85 (85,2%) 37,0% 370 37 0 (37,0 185 18 18,5% respectivamente. respectivamente respectivamente) 537 53 7 53,7% 88,9%. 889 88,9% 88 9 (88,9%) 33,3%. Conclusão qualificados entanto intervenção fisiológicas 202 33,3 (33,3% 22,2 (22,2% 85,2 8 (85,2% 37,0 (37, 18,5 53,7 88,9 (88,9% 20 33, (33,3 22, (22,2 85, (85,2 37, (37 18, 53, 88, (88,9 (33, (22, (85, (3 (88, (33 (22 (85 ( (88 (2 (8

Abstract Introduction: Exercise is being incorporated into the treatment of patients on hemodialysis; however, little is known about the major characteristics of these interventions. Objective: To describe the exercise protocols prescribed for hemodialysis patients in Brazil. Methods: A scoping review was conducted following JBI and Prisma-ScR guidelines. Searches were carried out in Medline, Embase and three other databases until May 2024. Other sources (websites, books and guidelines) were also investigated. Evidence from patients on hemodialysis describing exercise protocols in all settings and designs in Brazil was included. Results: Forty-five pieces of evidence were found, resulting in 54 exercise protocols from 16 Brazilian states. Strength exercises (33.3%), followed by aerobic exercises (22.2%), were the most prescribed, mainly to be performed during dialysis (85.2%). The most prevalent professionals supervising the programs were physiotherapists and exercise physiologists (37.0% and 18.5%, respectively). All protocols implemented the principles of type and frequency training, while progression was adopted in only 53.7%. The main prescription was three times per week (88.9%). Exercise intensity was predominantly determined by subjective methods (33.3%). Conclusion: Aerobic and strength exercises during dialysis were the most commonly prescribed modalities in Brazil, with the majority of programs being properly supervised by qualified professionals. However, existing protocols have not employed systematic progression throughout the intervention, which would be appropriate for providing better physiological responses and adaptations. Introduction however interventions Objective Methods PrismaScR Prisma ScR guidelines Medline 2024 websites, websites (websites investigated included Results Fortyfive Forty five found 5 1 states 33.3%, 333 33.3% , 33 3 (33.3%) 22.2%, 222 22.2% 22 2 (22.2%) 85.2%. 852 85.2% . 85 (85.2%) 37.0% 370 37 0 (37.0 185 18 18.5% respectively. respectively respectively) training 537 53 7 53.7% 88.9%. 889 88.9% 88 9 (88.9%) 33.3%. Conclusion However intervention adaptations 202 33.3 (33.3% 22.2 (22.2% 85.2 8 (85.2% 37.0 (37. 18.5 53.7 88.9 (88.9% 20 33. (33.3 22. (22.2 85. (85.2 37. (37 18. 53. 88. (88.9 (33. (22. (85. (3 (88. (33 (22 (85 ( (88 (2 (8

RESUMO A contaminação do solo com metais tóxicos traz consigo graves problemas ambientais. Dentre esses metais, o cobre (Cu) é prejudicial ao desenvolvimento das plantas quando atinge elevados níveis de contaminação no solo. Assim, identificar espécies capazes de resistir a esse tipo de contaminação auxilia nos processos de revegetação e descontaminação desses locais. Portanto, o presente estudo teve como objetivo investigar a tolerância de plantas de Cedrela fissilis ao excesso de Cu através de variáveis morfofisiológicas e bioquímicas. Mudas de C. fissilis foram submetidas a cinco concentrações de Cu (0, 2, 4, 6 e 8 mg L-1). O delineamento experimental foi inteiramente casualizado, com cinco tratamentos e quatro repetições. Características morfofisiológicas (número de folhas, altura da parte aérea, comprimento da raiz, massa seca, variáveis morfológicas do sistema radicular e área foliar) e bioquímicas (enzimas antioxidantes, peroxidação lipídica, concentração de peróxido de hidrogênio e pigmentos fotossintéticos) e Cu acumulado nas raízes e folhas foram avaliados. Altas concentrações de Cu tiveram efeito negativo no peso seco da parte aérea e das raízes, na taxa fotossintética, na condutância estomática e na taxa transpiratória. No geral, o Cu aumentou a atividade das enzimas antioxidantes e a peroxidação lipídica. Portanto, a incidência de Cu na solução nutritiva influenciou negativamente as características bioquímicas e fisiológicas das mudas de C. fissilis em detrimento do seu crescimento. Assim, foi possível identificar o comportamento sensível da espécie investigada. Devido a essas características, mudas de C. fissilis podem ser utilizadas como bioindicadoras de áreas contaminadas por cobre. ambientais (Cu Assim locais Portanto C 0, 0 (0 2 4 L1. L1 L 1 . L-1) casualizado repetições número raiz seca foliar lipídica fotossintéticos avaliados fotossintética transpiratória geral crescimento investigada ( L-1 L-

ABSTRACT Soil contamination with toxic metals brings along severe environmental issues. Among these metals, copper (Cu) is harmful to plant development when it reaches high contamination levels in the soil. Thus, identifying species capable of resisting this contamination type helps these sites’ revegetation and decontamination processes. Therefore, the present study aimed to investigate the tolerance of Cedrela fissilis plants to excess Cu through morphophysiological and biochemical variables. C. fissilis seedlings were subjected to five concentrations of Cu (0, 2, 4, 6, and 8 mg L-1). The experimental design was completely randomized, with five treatments and four repetitions. Morphophysiological (number of leaves, shoot height, root length, dry weight, morphological variables of the root system, and leaf area) and biochemical (antioxidant enzymes, lipid peroxidation, hydrogen peroxide concentration, and photosynthetic pigments) traits and Cu accumulated in roots and shoot were evaluated. High concentrations of Cu had a negative effect on the shoot and root dry weight, photosynthetic rate, stomatal conductance, and transpiration rate. Overall, Cu increased the activity of the antioxidant enzymes and lipid peroxidation. Therefore, Cu incidence in the nutrient solution has negatively influenced the biochemical and physiological traits of C. fissilis seedlings to the detriment of their growth. Thus, it was possible to identify sensitive behavior by the investigated species. Because of these features, C. fissilis seedlings can be used as markers for copper-contaminated areas. issues (Cu soil Thus sites processes Therefore C 0, 0 (0 2 4 6 L1. L1 L 1 . L-1) randomized repetitions number leaves height length weight system area peroxidation concentration pigments evaluated rate conductance Overall growth features coppercontaminated contaminated areas ( L-1 L-

RESUMO O potássio é um nutriente com potencial para aumentar a tolerância das plantas ao estresse salino. O objetivo deste estudo foi avaliar a produção e a qualidade da couve submetida ao estresse salino e às concentrações de potássio em ambiente protegido. O delineamento experimental adotado foi em parcela subdivididas, sendo as parcelas compostas por cinco soluções nutritivas (tratamento controle, S1 - solução nutritiva padrão usando água de baixa salinidade, 0,5 dS m-1 (500 mg L-1 de KNO3), e quatro soluções nutritivas preparadas em águas salobras (3,5 dS m-1) contendo quatro concentrações de KNO3 (S2 - 500 mg L-1, S3 - 625 mg L-1, S4 - 750 mg L-1, S5 - 1.000 mg L-1). As subparcelas foram representadas por duas cultivares de couve folha (Manteiga e Manteiga da Geórgia). Foram avaliadas as variáveis de produção de folhas e qualidades físicas e químicas pós-colheita. A solução nutritiva salinizada com adição de NaCl (S2) reduziu a produção, mas não reduziu a qualidade pós-colheita da couve folha. A adição extra de KNO3 em 25% (S3) foi eficiente para reduzir o efeito deletério do estresse salino sobre as variáveis comprimento de folha, área foliar, produção e número de maços. A cv. Manteiga da Geórgia se mostrou mais tolerante ao estresse salino que a cv. Manteiga. protegido subdivididas tratamento controle S salinidade 05 0 5 0, m1 m 1 m- (50 L1 L L- KNO3, KNO , KNO3) 3,5 35 3 (3, S2 (S 50 L1, 1, 62 75 1000 000 1.00 L1. . L-1) Geórgia. Geórgia) póscolheita. póscolheita pós colheita. colheita 25 (S3 foliar maços cv (5 3, (3 6 7 100 00 1.0 2 ( 10 1.

ABSTRACT Potassium is a nutrient with the potential to increase plant tolerance to salt stress. The aim of this study was to evaluate the production and quality of kale subjected to salt stress and potassium concentrations in a protected environment. The experimental design was in split plots, with plots composed of five nutrient solutions (control treatment, S1 - standard nutrient solution prepared in low-salinity water, 0.5 dS m-1 (500 mg L-1 of KNO3), and four nutrient solutions prepared in brackish waters (3.5 dS m-1) containing four concentrations of KNO3 (S2 - 500 mg L-1, S3 - 625 mg L-1, S4 - 750 mg L-1, S5 - 1,000 mg L-1), and subplots represented by two leaf kale cultivars (Manteiga and Manteiga da Geórgia). Leaf production variables and post-harvest physical and chemical qualities were evaluated. The saline nutrient solution with the addition of NaCl (S2) reduced production but did not reduce the post-harvest quality of kale. The extra addition of KNO3 at 25% (S3) was efficient in reducing the deleterious effects of salt stress on the variables leaf length, leaf area, leaf production, and number of bunches. The cv. Manteiga da Geórgia was more tolerant to salt stress than the cv. Manteiga. environment control treatment S lowsalinity low salinity water 05 0 5 0. m1 m 1 m- (50 L1 L L- KNO3, KNO , KNO3) 3.5 35 3 (3. S2 (S 50 L1, 1, 62 75 1000 000 1,00 L-1) Geórgia. . Geórgia) postharvest post harvest evaluated 25 (S3 length area bunches cv (5 3. (3 6 7 100 00 1,0 2 ( 10